CN105418787A - Extraction method for polysaccharides in moringa oleifera seeds - Google Patents
Extraction method for polysaccharides in moringa oleifera seeds Download PDFInfo
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- CN105418787A CN105418787A CN201511008885.0A CN201511008885A CN105418787A CN 105418787 A CN105418787 A CN 105418787A CN 201511008885 A CN201511008885 A CN 201511008885A CN 105418787 A CN105418787 A CN 105418787A
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- moringa seeds
- polysaccharides
- polysaccharide
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- moringa
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention relates to an extraction method for polysaccharides in moringa oleifera seeds. The extraction method is aimed to solve the problem of low yield of a moringa oleifera seed polysaccharide extract solution in present extraction methods for polysaccharides in moringa oleifera seeds. The extraction method is as follows: powdery moringa oleifera seed is placed in water and is subjected to extraction for certain time at a certain temperature, and a moringa oleifera seed polysaccharide extract solution is prepared. The yield of the polysaccharides in the moringa oleifera seed polysaccharide extract solution reaches above 14%. The method is advantageous in that the ratio of the moringa oleifera seed weight to the water volume and the extraction problem and time are controlled strictly, thus the yield of the polysaccharides in the moringa oleifera seed polysaccharide extract solution reaches above 14%, and water resource consumption is reduced. The method is mainly used for extracting polysaccharides in moringa oleifera seeds.
Description
Technical field
The present invention relates to extraction method of polysaccharides in a kind of moringa seeds.
Background technology
Moringa is a kind of tropical plants with abundant nutrition and unique economic value, is popular in the international market; According to safe Jerry Millner all College of Horticultures of agriculture university of India, Moringa root crust can not use because containing two kinds of alkaloids, in addition, and the equal edible of Moringa complete stool, micro-pungent, the tepor of its taste and nontoxic.Moringa is not only the desirable food of vegetarian, solves the natural nutrition storehouse of poor regional nutritive deficiency problem especially.There is anti-inflammatory, pain relieving, step-down, protect the effects such as liver, cardiac stimulant, diuresis, lactagogue, more than its effect, through its pharmacy conventional, with diseases such as prevention and therapy digestive system neoplasm, immunizing power underground, hypertension, vitamin C deficiency, anaemia, tetter, diabetes, obesity.
The extracting method of vegetable polysaccharides is generally boiling water extraction, and the cycle is long, and temperature is high, and liquid ratio is large, is unfavorable for suitability for industrialized production.In current existing moringa seeds, the extracting method of polysaccharide is: the liquid ratio of moringa seeds and water is: 30-80mL/g; Extracting temperature is 100 DEG C, and extraction time is 1-4 hour, and the productive rate obtaining polysaccharide in moringa seeds polysaccharide extraction liquid is only up to 9.83%.
Summary of the invention
There is the low problem of the productive rate of polysaccharide in moringa seeds polysaccharide extraction liquid in the extracting method that the object of the invention is to solve polysaccharide in existing moringa seeds; And extraction method of polysaccharides in a kind of moringa seeds is provided.
Extraction method of polysaccharides in a kind of moringa seeds, specifically completes according to the following steps: Powdered moringa seeds is put into water, extracts 1h ~ 1.10h, obtain moringa seeds polysaccharide extraction liquid at temperature is 78 ~ 79 DEG C; In described moringa seeds polysaccharide extraction liquid, the productive rate of polysaccharide reaches more than 14%; The quality of described Powdered moringa seeds and the volume ratio of water are 1g:(55mL ~ 56mL).
Advantage of the present invention: the volume of quality and water that moringa seeds is strictly controlled in the present invention when extracts problem and time, makes the productive rate of polysaccharide in moringa seeds polysaccharide extraction liquid reach more than 14%, and decreases the consumption of water resources.
Embodiment
Embodiment one: present embodiment is extraction method of polysaccharides in a kind of moringa seeds, specifically completes according to the following steps: Powdered moringa seeds is put into water, extracts 1h ~ 1.10h, obtains moringa seeds polysaccharide extraction liquid at temperature is 78 ~ 79 DEG C; In described moringa seeds polysaccharide extraction liquid, the productive rate of polysaccharide reaches more than 14%; The quality of described Powdered moringa seeds and the volume ratio of water are 1g:(55mL ~ 56mL).
The extracting method of vegetable polysaccharides is generally boiling water extraction, and therefore in existing moringa seeds, the Extracting temperature of the extracting method of polysaccharide is 100 DEG C, and general assert that the time of extracting, the longer the better, thus in existing moringa seeds the time of the extracting method of polysaccharide reach 4h most, present embodiment jumps out existing thinking, the quality of moringa seeds and the volume ratio (being called for short: liquid ratio) of water is related to extraction method of polysaccharides in moringa seeds, Extracting temperature and extraction time are as three changed factors, DesignExpert software is utilized to be optimized according to Box-Behnken principle of design, Box-Behnken (BBD) center combination design model is Responds Surface Methodology, with three changed factors, only by three levels, compared with Orthogonal Method, just can obtain a result by a small amount of experimental group, and the top condition obtained be not setting value but within the scope imposed a condition.By taking response phase method to analyze having ready conditions most of moringa seeds Polyose extraction, by being optimized extraction time, extraction temperature and the solid-liquid ratio influence factor to moringa seeds polysaccharide extract rate, obtain best optimal conditions.
Embodiment two: present embodiment and embodiment one difference are: described Powdered moringa seeds is prepared according to the following steps: moringa seeds is first through degreasing, deproteinated and depigmentation process, again successively through super-dry and grinding, namely obtain Powdered moringa seeds.Other are identical with embodiment one.
Embodiment three: one of present embodiment and embodiment one or two difference is: described Powdered moringa seeds water ratio≤8%.Other are identical with embodiment one or two.
Embodiment four: one of present embodiment and embodiment one to three difference is: extract 1h ~ 1.10h at temperature is 78.5 DEG C.Other are identical with embodiment one to three.
Embodiment five: one of present embodiment and embodiment one to four difference is: extract 1.08h at temperature is 78 ~ 79 DEG C.Other are identical with embodiment one to four.
Embodiment six: one of present embodiment and embodiment one to five difference is: the quality of described Powdered moringa seeds and the volume ratio of water are 1g:55.6mL.Other are identical with embodiment one to five.
Embodiment seven: one of present embodiment and embodiment one to six difference is: moringa seeds is dried to water ratio≤8%, then pulverize, cross 80 mesh sieves, obtain Powdered moringa seeds, Powdered moringa seeds is put into water, at temperature is 78.5 DEG C, extracts 1.08h, obtains moringa seeds polysaccharide extraction liquid; In described moringa seeds polysaccharide extraction liquid, the productive rate of polysaccharide is 14.31%; The quality of described moringa seeds and the volume ratio of water are 1g:55.6mL.Other are identical with embodiment one to six.
Content of the present invention is not limited only to the content of the respective embodiments described above, and the combination of one of them or several embodiment equally also can realize the object of inventing.
Adopt following verification experimental verification effect of the present invention
Experiment 1, a kind of moringa seeds extraction method of polysaccharides, comprise the steps:
Moringa seeds is dried to water ratio≤8%, then pulverizes, cross 80 mesh sieves, obtain Powdered moringa seeds, Powdered for 1g moringa seeds is put into 55.6mL deionized water, at temperature is 78.5 DEG C, extracts 1.08h, obtains moringa seeds polysaccharide extraction liquid.
Under temperature is 15 DEG C and rotating speed is 4000rpm, carry out centrifugation 20min to moringa seeds polysaccharide extraction liquid, supernatant liquor reclaims, and uses deionization constant volume, obtains determinand, with the content of polysaccharide in the phend-sulphuric acid quantitative assay determinand of improvement.Use the formulae discovery rate of output: productive rate (%)=[extract concentrations × constant volume/raw material dry weight] × 100%; Be 14.31% by the productive rate of polysaccharide in the moringa seeds polysaccharide extraction liquid that calculates known experiment 1 preparation.
The phend-sulphuric acid of improvement derives from open source literature " phend-sulphuric acid of improvement measures the research of polysaccharide and oligosaccharide content "; " Chinese Pharmaceutical Journal ", 1996 (9): 550-553.
Experiment 2, the impact of extraction time change on polysaccharide yield
Moringa seeds is dried to water ratio≤8%, then pulverizes, cross 80 mesh sieves, obtain Powdered moringa seeds, Powdered for 1g moringa seeds is put into 55.6mL deionized water, at temperature is 78.5 DEG C, extracts 2h, obtains moringa seeds polysaccharide extraction liquid.
Be 13.21% by the productive rate of polysaccharide in the moringa seeds polysaccharide extraction liquid that calculates known experiment 2 preparation.
Experiment 3, the impact of Extracting temperature change on polysaccharide yield:
Moringa seeds is dried to water ratio≤8%, then pulverizes, cross 80 mesh sieves, obtain Powdered moringa seeds, Powdered for 1g moringa seeds is put into 55.6mL deionized water, at temperature is 70 DEG C, extracts 1.08h, obtains moringa seeds polysaccharide extraction liquid.
Be 13.89% by the productive rate of polysaccharide in the moringa seeds polysaccharide extraction liquid that calculates known experiment 3 preparation.
Experiment 4, the impact of extraction liquid ratio change on polysaccharide yield
Moringa seeds is dried to water ratio≤8%, then pulverizes, cross 80 mesh sieves, obtain Powdered moringa seeds, Powdered for 1g moringa seeds is put into 40mL deionized water, at temperature is 78.5 DEG C, extracts 1.08h, obtains moringa seeds polysaccharide extraction liquid.
Be 10.24% by the productive rate of polysaccharide in the moringa seeds polysaccharide extraction liquid that calculates known experiment 4 preparation.
Claims (7)
1. an extraction method of polysaccharides in moringa seeds, is characterized in that it completes according to the following steps: Powdered moringa seeds is put into water, extracts 1h ~ 1.10h, obtain moringa seeds polysaccharide extraction liquid at temperature is 78 ~ 79 DEG C; In described moringa seeds polysaccharide extraction liquid, the productive rate of polysaccharide reaches more than 14%; The quality of described Powdered moringa seeds and the volume ratio of water are 1g:(55mL ~ 56mL).
2. extraction method of polysaccharides in a kind of moringa seeds according to claim 1, it is characterized in that described Powdered moringa seeds is prepared according to the following steps: moringa seeds is first through degreasing, deproteinated and depigmentation process, again successively through super-dry and grinding, namely obtain Powdered moringa seeds.
3. extraction method of polysaccharides in a kind of moringa seeds according to claim 2, is characterized in that described Powdered moringa seeds water ratio≤8%.
4. extraction method of polysaccharides in a kind of moringa seeds according to claim 3, is characterized in that extracting 1h ~ 1.10h at temperature is 78.5 DEG C.
5. extraction method of polysaccharides in a kind of moringa seeds according to claim 3, it is characterized in that extracting 1.08h at temperature is 78 ~ 79 DEG C.
6. extraction method of polysaccharides in a kind of moringa seeds according to claim 3, is characterized in that the quality of described Powdered moringa seeds and the volume ratio of water are 1g:55.6mL.
7. extraction method of polysaccharides in a kind of moringa seeds according to claim 2, moringa seeds is it is characterized in that to be dried to water ratio≤8%, then pulverize, cross 80 mesh sieves, obtain Powdered moringa seeds, Powdered moringa seeds is put into water, at temperature is 78.5 DEG C, extracts 1.08h, obtains moringa seeds polysaccharide extraction liquid; In described moringa seeds polysaccharide extraction liquid, the productive rate of polysaccharide is 14.31%; The quality of described moringa seeds and the volume ratio of water are 1g:55.6mL.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108373512A (en) * | 2018-05-08 | 2018-08-07 | 桂林理工大学 | A method of the depth congruent melting solvent extraction Moringa seed polysaccharide made of choline chloride and urea |
| CN108676102A (en) * | 2018-05-08 | 2018-10-19 | 桂林理工大学 | A method of the depth congruent melting solvent extraction Moringa seed polysaccharide made of choline chloride and glycerine |
| CN108752489A (en) * | 2018-05-08 | 2018-11-06 | 桂林理工大学 | A method of the depth congruent melting solvent extraction Moringa seed polysaccharide made of choline chloride and ethylene glycol |
| CN108771074A (en) * | 2018-05-17 | 2018-11-09 | 广西柳州市桑辰科技有限公司 | A kind of production method of moringa seeds solid beverage |
| CN110003352A (en) * | 2019-04-12 | 2019-07-12 | 北部湾大学 | A kind of extracting method that moringa seeds selenium active constituent is effectively kept |
| CN110721134A (en) * | 2019-11-29 | 2020-01-24 | 广州环亚化妆品科技有限公司 | Method for extracting tyrosinase inhibitor from moringa seeds, tyrosinase inhibitor prepared by same and application thereof |
| CN113024679B (en) * | 2021-02-25 | 2022-11-08 | 广西壮族自治区农业科学院 | Method for extracting selenium polysaccharide and polyphenol from selenium-rich moringa seeds |
| CN115521384A (en) * | 2022-09-28 | 2022-12-27 | 无锡绿色分离应用技术研究所有限公司 | High-efficiency extraction method of moringa oleifera polysaccharide |
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| CN102617747A (en) * | 2012-03-26 | 2012-08-01 | 浙江大学 | Extracting process of dendrobium candidum polysaccharides and method for optimizing process by response surface method |
| CN104829743A (en) * | 2015-05-25 | 2015-08-12 | 萧丽雅 | Preparation method and use of moringa oleifera leaf polysaccharides |
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| CN102617747A (en) * | 2012-03-26 | 2012-08-01 | 浙江大学 | Extracting process of dendrobium candidum polysaccharides and method for optimizing process by response surface method |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108373512A (en) * | 2018-05-08 | 2018-08-07 | 桂林理工大学 | A method of the depth congruent melting solvent extraction Moringa seed polysaccharide made of choline chloride and urea |
| CN108676102A (en) * | 2018-05-08 | 2018-10-19 | 桂林理工大学 | A method of the depth congruent melting solvent extraction Moringa seed polysaccharide made of choline chloride and glycerine |
| CN108752489A (en) * | 2018-05-08 | 2018-11-06 | 桂林理工大学 | A method of the depth congruent melting solvent extraction Moringa seed polysaccharide made of choline chloride and ethylene glycol |
| CN108771074A (en) * | 2018-05-17 | 2018-11-09 | 广西柳州市桑辰科技有限公司 | A kind of production method of moringa seeds solid beverage |
| CN108771074B (en) * | 2018-05-17 | 2021-08-31 | 广西贺州市桑辰科技有限公司 | Preparation method of moringa seed solid beverage |
| CN110003352A (en) * | 2019-04-12 | 2019-07-12 | 北部湾大学 | A kind of extracting method that moringa seeds selenium active constituent is effectively kept |
| CN110721134A (en) * | 2019-11-29 | 2020-01-24 | 广州环亚化妆品科技有限公司 | Method for extracting tyrosinase inhibitor from moringa seeds, tyrosinase inhibitor prepared by same and application thereof |
| CN113024679B (en) * | 2021-02-25 | 2022-11-08 | 广西壮族自治区农业科学院 | Method for extracting selenium polysaccharide and polyphenol from selenium-rich moringa seeds |
| CN115521384A (en) * | 2022-09-28 | 2022-12-27 | 无锡绿色分离应用技术研究所有限公司 | High-efficiency extraction method of moringa oleifera polysaccharide |
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