CN115521384A - High-efficiency extraction method of moringa oleifera polysaccharide - Google Patents
High-efficiency extraction method of moringa oleifera polysaccharide Download PDFInfo
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- 235000011347 Moringa oleifera Nutrition 0.000 title claims abstract description 200
- 238000000605 extraction Methods 0.000 title claims abstract description 142
- 244000179886 Moringa oleifera Species 0.000 title claims abstract description 108
- 150000004676 glycans Chemical class 0.000 title claims abstract description 97
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 97
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 97
- 241000220215 Moringa Species 0.000 claims abstract description 92
- 239000002994 raw material Substances 0.000 claims abstract description 7
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 54
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 36
- 238000000034 method Methods 0.000 claims description 31
- 230000004044 response Effects 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 18
- 235000006408 oxalic acid Nutrition 0.000 claims description 18
- 238000002791 soaking Methods 0.000 claims description 12
- 238000002137 ultrasound extraction Methods 0.000 claims description 12
- 238000004140 cleaning Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000007781 pre-processing Methods 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- 238000007873 sieving Methods 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 12
- 238000005520 cutting process Methods 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003225 biodiesel Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Chemical Kinetics & Catalysis (AREA)
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a high-efficiency extraction method of moringa oleifera polysaccharide. In the invention, the raw material is firstly extracted and preprocessed, the moringa is divided into moringa leaves, moringa roots and moringa seeds, and the divided moringa leaves, moringa roots and moringa seeds are cleaned, dried, crushed and sieved for later use; the extraction of the moringa oleifera polysaccharides in the moringa oleifera leaves is carried out, the moringa oleifera polysaccharides in the moringa oleifera leaves are extracted by adopting ultrasonic waves, different extraction modes are adopted for different parts of the moringa oleifera, so that the extraction efficiency of each part is improved, the extraction rate of the whole moringa oleifera polysaccharides is improved, different extraction modes are adopted simultaneously, the production and extraction cost is reduced as much as possible, the economy of the extraction mode is improved, the extraction steps are simple to operate, the operation difficulty is reduced for the production process, and the convenience of the extraction method in operation is improved.
Description
Technical Field
The invention belongs to the technical field of moringa polysaccharide, and particularly relates to an efficient extraction method of moringa polysaccharide.
Background
In recent years, the development and utilization of moringa oleifera are mainly focused on aspects of functional food, wastewater treatment, plant protein feed, biodiesel, cosmetics and the like. The moringa oleifera resource is rich in China, and a material basis is provided for the development and application of moringa oleifera polysaccharide. Under the condition that the state greatly supports the development of health industry in Bay of hongkong, hong Kong and Australia, the moringa polysaccharide can be used as functional food or health-care product additive in the future because of the various physiological activities of reducing blood sugar and blood fat, resisting inflammation, resisting tumor and the like. Meanwhile, the antioxidant has good antioxidant effect and can be used as an antioxidant. Besides, it can also be used as feed additive. Therefore, in view of the potential market development prospect of moringa oleifera, the moringa oleifera industry should be vigorously developed in various fields.
During extraction of common moringa polysaccharide, different parts adopt the same extraction method, so that the extraction cost is higher, and the extraction efficiency is not high enough.
Disclosure of Invention
The invention aims to: in order to solve the problems, a high-efficiency extraction method of moringa oleifera polysaccharide is provided.
The technical scheme adopted by the invention is as follows: a high-efficiency extraction method of moringa oleifera polysaccharide comprises the following steps:
s1, extracting raw materials, preprocessing, cutting the moringa oleifera into moringa leaves, moringa roots and moringa seeds, cleaning, drying, crushing and screening the cut moringa leaves, moringa roots and moringa seeds for later use;
s2, extracting moringa oleifera polysaccharide in moringa oleifera leaves, extracting the moringa oleifera polysaccharide in the moringa oleifera leaves by adopting ultrasonic waves, weighing 10-30 parts of the moringa oleifera leaves and 150-400 parts of hot water, adding 30-50 parts of 0.03g/mL metaphosphoric acid-acetic acid solution, 150-400 parts of 10% hydrochloric acid solution and 150-400 parts of 2% oxalic acid solution, and adjusting the pH value to 3;
s3, extracting moringa oleifera leaf polysaccharide by using an ultrasonic extraction device,
s4, extracting moringa polysaccharide from moringa roots, preparing an extracting solution, measuring 150-400 parts of hot water, adding 20-40 parts of metaphosphoric acid-acetic acid solution of 0.03g/mL, 200-300 parts of 10% hydrochloric acid solution and 160-300 parts of 2% oxalic acid solution, and adjusting the pH value to 3;
s5, placing the moringa roots in the extracting solution prepared in the step S4, raising the temperature of the extracting solution, beginning to soak and extract the moringa roots,
s6, extracting moringa oleifera polysaccharides in moringa oleifera seeds, optimizing the extraction of the moringa oleifera seeds polysaccharides by adopting a three-factor three-level response surface method, adjusting the pH value to 3,
s7, collecting and storing the moringa polysaccharides extracted in the steps to complete the preparation of the whole moringa polysaccharides.
In a preferred embodiment, in the step S1, the number of the sieved meshes is 200 to 300 meshes.
In a preferred embodiment, in the step S2, the temperature of the hot water for ultrasonic extraction of moringa polysaccharide is controlled to be 50 ℃.
In a preferred embodiment, in the step S3, the extraction frequency is controlled to be 2 times, and the extraction rate of the moringa oleifera leaf polysaccharide is controlled to be 13.30% when the single extraction time is controlled to be 30 min.
In a preferred embodiment, in step S5, the temperature of the extracting solution is controlled to be 75 ℃, the time of a single soaking extraction is controlled to be 180min, and the number of times of soaking extraction is controlled to be 4.
In a preferred embodiment, in the step S6, the temperature is controlled to be 80 degrees celsius when the three-factor three-level response surface method is adopted for extraction.
In a preferred embodiment, in the step S6, the formula of the extracting solution when the three-factor three-level response surface method is adopted for extraction is as follows: 150 to 400 portions of hot water, 100 to 200 portions of metaphosphoric acid-acetic acid solution of 0.03g/mL, 300 to 500 portions of 10 percent hydrochloric acid solution and 200 to 400 portions of 2 percent oxalic acid solution are added.
In a preferred embodiment, in the step S6, when the three-factor three-level response surface method is used for extraction, the extraction time is 1h, and the extraction rate of the moringa polysaccharides is 15.20% ± 0.30%.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
according to the invention, different extraction modes are adopted for different parts of moringa oleifera, so that the extraction efficiency of each part is improved, the extraction rate of the whole moringa oleifera polysaccharide is improved, meanwhile, the production and extraction costs are reduced as much as possible by adopting different extraction modes, so that the economy of the extraction mode is improved, the extraction steps are simple to operate, the operation difficulty is reduced for the production process, and the convenience of the extraction method in operation is improved.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
a high-efficiency extraction method of moringa polysaccharide comprises the following steps:
s1, extracting raw materials, preprocessing, cutting the moringa oleifera into moringa leaves, moringa roots and moringa seeds, cleaning, drying, crushing and screening the cut moringa leaves, moringa roots and moringa seeds for later use;
s2, extracting moringa oleifera polysaccharides in moringa oleifera leaves, extracting the moringa oleifera polysaccharides in the moringa oleifera leaves by adopting ultrasonic waves, weighing 10 parts of the moringa oleifera leaves and 150 parts of hot water, adding 30 parts of a metaphosphoric acid-acetic acid solution of 0.03g/mL, 150 parts of a 10% hydrochloric acid solution and 150 parts of a 2% oxalic acid solution, and adjusting the pH value to be 3;
s3, extracting moringa oleifera leaf polysaccharide by using an ultrasonic extraction device,
s4, extracting moringa polysaccharide from moringa roots, preparing an extracting solution, measuring 150-400 parts of hot water, adding 20 parts of 0.03g/mL metaphosphoric acid-acetic acid solution, 200 parts of 10% hydrochloric acid solution and 160 parts of 2% oxalic acid solution, and adjusting the pH value to 3;
s5, placing the moringa roots in the extracting solution prepared in the step S4, raising the temperature of the extracting solution, beginning to soak and extract the moringa roots,
s6, extracting moringa oleifera polysaccharides in moringa oleifera seeds, optimizing the extraction of the moringa oleifera seeds polysaccharides by adopting a three-factor three-level response surface method, adjusting the pH value to 3,
s7, collecting and storing the moringa polysaccharide extracted in the steps to finish the preparation of the whole moringa polysaccharide.
In the step S1, the sieving mesh number is 200-300 meshes.
In the step S2, the hot water temperature of the moringa oleifera polysaccharide subjected to ultrasonic extraction is controlled to be 50 ℃.
In the step S3, the extraction frequency is controlled to be 2 times, and the extraction rate of the moringa oleifera leaf polysaccharide is controlled to be 13.30% when the single extraction time is controlled to be 30 min.
In the step S5, the temperature of the extracting solution is controlled to be 75 ℃, the time of single soaking extraction is controlled to be 180min, and the times of soaking extraction are controlled to be 4 times.
In the step S6, the temperature is controlled to be 80 ℃ when the three-factor three-level response surface method is adopted for extraction.
In the step S6, the formula of the extracting solution when the three-factor three-level response surface method is adopted for extraction is as follows: 150 parts of hot water, 100 parts of metaphosphoric acid-acetic acid solution of 0.03g/mL, 300 parts of 10% hydrochloric acid solution and 200 parts of 2% oxalic acid solution.
In the step S6, when a three-factor three-level response surface method is adopted for extraction, the extraction time is 1 hour, and the extraction rate of the moringa oleifera polysaccharide is 15.20 +/-0.30%.
According to the invention, different extraction modes are adopted for different parts of moringa oleifera, so that the extraction efficiency of each part is improved, the extraction rate of the whole moringa oleifera polysaccharide is improved, meanwhile, the production and extraction costs are reduced as much as possible by adopting different extraction modes, so that the economy of the extraction mode is improved, the extraction steps are simple to operate, the operation difficulty is reduced for the production process, and the convenience of the extraction method in operation is improved.
Example two:
a high-efficiency extraction method of moringa oleifera polysaccharide comprises the following steps:
s1, firstly, preprocessing raw material extraction, dividing the moringa oleifera into moringa leaves, moringa roots and moringa seeds, cleaning, drying, crushing and screening the divided moringa leaves, moringa roots and moringa seeds for later use;
s2, extracting moringa oleifera polysaccharide in moringa oleifera leaves, extracting the moringa oleifera polysaccharide in the moringa oleifera leaves by adopting ultrasonic waves, weighing 20 parts of the moringa oleifera leaves and 250 parts of hot water, adding 40 parts of 0.03g/mL metaphosphoric acid-acetic acid solution, 250 parts of 10% hydrochloric acid solution and 150 parts of 2% oxalic acid solution, and adjusting the pH value to 3;
s3, extracting moringa oleifera leaf polysaccharide by using an ultrasonic extraction device,
s4, extracting moringa polysaccharide from moringa roots, preparing an extracting solution, measuring 150-400 parts of hot water, adding 20 parts of 0.03g/mL metaphosphoric acid-acetic acid solution, 200 parts of 10% hydrochloric acid solution and 160 parts of 2% oxalic acid solution, and adjusting the pH value to 3;
s5, placing the moringa roots in the extracting solution prepared in the step S4, raising the temperature of the extracting solution, beginning to soak and extract the moringa roots,
s6, extracting moringa oleifera polysaccharides in moringa oleifera seeds, optimizing the extraction of the moringa oleifera seeds polysaccharides by adopting a three-factor three-level response surface method, adjusting the pH value to 3,
s7, collecting and storing the moringa polysaccharides extracted in the steps to complete the preparation of the whole moringa polysaccharides.
In the step S1, the sieving mesh number is 200-300 meshes.
In the step S2, the hot water temperature of the moringa oleifera polysaccharide subjected to ultrasonic extraction is controlled to be 50 ℃.
In the step S3, the extraction times are controlled to be 2 times, and the extraction rate of the moringa oleifera leaf polysaccharide reaches 13.30% after the single extraction time is controlled to be 30 min.
In the step S5, the temperature of the extracting solution is controlled to be 75 ℃, the time of single soaking extraction is controlled to be 180min, and the times of soaking extraction are controlled to be 4 times.
In the step S6, the temperature is controlled to be 80 ℃ when the three-factor three-level response surface method is adopted for extraction.
In the step S6, the formula of the extracting solution when the three-factor three-level response surface method is adopted for extraction is as follows: 150 parts of hot water, 100 parts of 0.03g/mL metaphosphoric acid-acetic acid solution, 300 parts of 10% hydrochloric acid solution and 200 parts of 2% oxalic acid solution.
In the step S6, when a three-factor three-level response surface method is adopted for extraction, the extraction time is 1 hour, and the extraction rate of the moringa oleifera polysaccharides is 15.20 +/-0.30%.
According to the invention, different extraction modes are adopted for different parts of moringa oleifera, so that the extraction efficiency of each part is improved, the extraction rate of the whole moringa oleifera polysaccharide is improved, meanwhile, the production and extraction costs are reduced as much as possible by adopting different extraction modes, so that the economy of the extraction mode is improved, the extraction steps are simple to operate, the operation difficulty is reduced for the production process, and the convenience of the extraction method in operation is improved.
Example three:
a high-efficiency extraction method of moringa polysaccharide comprises the following steps:
s1, extracting raw materials, preprocessing, cutting the moringa oleifera into moringa leaves, moringa roots and moringa seeds, cleaning, drying, crushing and screening the cut moringa leaves, moringa roots and moringa seeds for later use;
s2, extracting moringa oleifera polysaccharide in moringa oleifera leaves, extracting the moringa oleifera polysaccharide in the moringa oleifera leaves by adopting ultrasonic waves, weighing 30 parts of the moringa oleifera leaves and 250 parts of hot water, adding 50 parts of 0.03g/mL metaphosphoric acid-acetic acid solution, 400 parts of 10% hydrochloric acid solution and 250 parts of 2% oxalic acid solution, and adjusting the pH value to 3;
s3, extracting moringa oleifera leaf polysaccharide by using an ultrasonic extraction device,
s4, extracting moringa polysaccharide from moringa roots, preparing an extracting solution, measuring 150-400 parts of hot water, adding 20 parts of 0.03g/mL metaphosphoric acid-acetic acid solution, 200 parts of 10% hydrochloric acid solution and 160 parts of 2% oxalic acid solution, and adjusting the pH value to 3;
s5, placing the moringa roots in the extracting solution prepared in the step S4, raising the temperature of the extracting solution, starting to soak and extract the moringa roots,
s6, extracting moringa oleifera polysaccharides in moringa oleifera seeds, optimizing the extraction of the moringa oleifera seeds polysaccharides by adopting a three-factor three-level response surface method, adjusting the pH value to 3,
s7, collecting and storing the moringa polysaccharides extracted in the steps to complete the preparation of the whole moringa polysaccharides.
In the step S1, the sieving mesh number is 200-300 meshes.
In the step S2, the temperature of hot water for ultrasonic extraction of moringa polysaccharide is controlled to be 50 ℃.
In the step S3, the extraction frequency is controlled to be 2 times, and the extraction rate of the moringa oleifera leaf polysaccharide is controlled to be 13.30% when the single extraction time is controlled to be 30 min.
In the step S5, the temperature of the extracting solution is controlled to be 75 ℃, the time of single soaking extraction is controlled to be 180min, and the times of soaking extraction are controlled to be 4 times.
In the step S6, the temperature is controlled to be 80 ℃ when the three-factor three-level response surface method is adopted for extraction.
In the step S6, the formula of the extracting solution when the three-factor three-level response surface method is adopted for extraction is as follows: 150 parts of hot water, 100 parts of 0.03g/mL metaphosphoric acid-acetic acid solution, 300 parts of 10% hydrochloric acid solution and 200 parts of 2% oxalic acid solution.
In the step S6, when a three-factor three-level response surface method is adopted for extraction, the extraction time is 1 hour, and the extraction rate of the moringa oleifera polysaccharides is 15.20 +/-0.30%.
According to the invention, different extraction modes are adopted for different parts of moringa oleifera, so that the extraction efficiency of each part is improved, the extraction rate of the whole moringa oleifera polysaccharide is improved, meanwhile, the production and extraction costs are reduced as much as possible by adopting different extraction modes, so that the economy of the extraction mode is improved, the extraction steps are simple to operate, the operation difficulty is reduced for the production process, and the convenience of the extraction method in operation is improved.
Example four:
a high-efficiency extraction method of moringa oleifera polysaccharide comprises the following steps:
s1, extracting raw materials, preprocessing, cutting the moringa oleifera into moringa leaves, moringa roots and moringa seeds, cleaning, drying, crushing and screening the cut moringa leaves, moringa roots and moringa seeds for later use;
s2, extracting moringa oleifera polysaccharide in moringa oleifera leaves, extracting the moringa oleifera polysaccharide in the moringa oleifera leaves by adopting ultrasonic waves, weighing 20 parts of the moringa oleifera leaves and 250 parts of hot water, adding 40 parts of 0.03g/mL metaphosphoric acid-acetic acid solution, 250 parts of 10% hydrochloric acid solution and 150 parts of 2% oxalic acid solution, and adjusting the pH value to 3;
s3, extracting moringa oleifera leaf polysaccharide by using an ultrasonic extraction device,
s4, extracting moringa polysaccharide from moringa roots, preparing an extracting solution, measuring 150-400 parts of hot water, adding 50 parts of 0.03g/mL metaphosphoric acid-acetic acid solution, 300 parts of 10% hydrochloric acid solution and 260 parts of 2% oxalic acid solution, and adjusting the pH value to 3;
s5, placing the moringa roots in the extracting solution prepared in the step S4, raising the temperature of the extracting solution, beginning to soak and extract the moringa roots,
s6, extracting moringa oleifera polysaccharides in moringa oleifera seeds, optimizing the extraction of the moringa oleifera seeds polysaccharides by adopting a three-factor three-level response surface method, adjusting the pH value to 3,
s7, collecting and storing the moringa polysaccharides extracted in the steps to complete the preparation of the whole moringa polysaccharides.
In the step S1, the sieving mesh number is 200-300 meshes.
In the step S2, the hot water temperature of the moringa oleifera polysaccharide subjected to ultrasonic extraction is controlled to be 50 ℃.
In the step S3, the extraction frequency is controlled to be 2 times, and the extraction rate of the moringa oleifera leaf polysaccharide is controlled to be 13.30% when the single extraction time is controlled to be 30 min.
In the step S5, the temperature of the extracting solution is controlled to be 75 ℃, the time of single soaking extraction is controlled to be 180min, and the times of soaking extraction are controlled to be 4 times.
In the step S6, the temperature is controlled to be 80 ℃ when the three-factor three-level response surface method is adopted for extraction.
In the step S6, the formula of the extracting solution when the three-factor three-level response surface method is adopted for extraction is as follows: 150 parts of hot water, 100 parts of 0.03g/mL metaphosphoric acid-acetic acid solution, 300 parts of 10% hydrochloric acid solution and 200 parts of 2% oxalic acid solution.
In the step S6, when a three-factor three-level response surface method is adopted for extraction, the extraction time is 1 hour, and the extraction rate of the moringa oleifera polysaccharides is 15.20 +/-0.30%.
According to the invention, different extraction modes are adopted for different parts of moringa oleifera, so that the extraction efficiency of each part is improved, the extraction rate of the whole moringa oleifera polysaccharide is improved, meanwhile, different extraction modes are adopted, the production extraction cost is reduced as much as possible, so that the economy of the extraction mode is improved, the extraction steps are simple to operate, the operation difficulty is reduced for the production process, and the convenience of the extraction method in operation is improved.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrases "comprising one of 8230; \8230;" 8230; "does not exclude the presence of additional like elements in a process, method, article, or apparatus that comprises the element.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (8)
1. A method for efficiently extracting moringa oleifera polysaccharide is characterized by comprising the following steps: the efficient extraction method of the moringa oleifera polysaccharide comprises the following steps:
s1, firstly, preprocessing raw material extraction, dividing the moringa oleifera into moringa leaves, moringa roots and moringa seeds, cleaning, drying, crushing and screening the divided moringa leaves, moringa roots and moringa seeds for later use;
s2, extracting moringa oleifera polysaccharide in moringa oleifera leaves, extracting the moringa oleifera polysaccharide in the moringa oleifera leaves by adopting ultrasonic waves, weighing 10-30 parts of the moringa oleifera leaves and 150-400 parts of hot water, adding 30-50 parts of 0.03g/mL metaphosphoric acid-acetic acid solution, 150-400 parts of 10% hydrochloric acid solution and 150-400 parts of 2% oxalic acid solution, and adjusting the pH value to 3;
s3, extracting moringa oleifera leaf polysaccharide by using an ultrasonic extraction device,
s4, extracting moringa polysaccharide from moringa roots, preparing an extracting solution, measuring 150-400 parts of hot water, adding 20-40 parts of metaphosphoric acid-acetic acid solution of 0.03g/mL, 200-300 parts of 10% hydrochloric acid solution and 160-300 parts of 2% oxalic acid solution, and adjusting the pH value to 3;
s5, placing the moringa roots in the extracting solution prepared in the step S4, raising the temperature of the extracting solution, beginning to soak and extract the moringa roots,
s6, extracting moringa oleifera polysaccharides in moringa oleifera seeds, optimizing the extraction of the moringa oleifera seeds polysaccharides by adopting a three-factor three-level response surface method, adjusting the pH value to 3,
s7, collecting and storing the moringa polysaccharide extracted in the steps to finish the preparation of the whole moringa polysaccharide.
2. The efficient extraction method of moringa polysaccharide as claimed in claim 1, wherein the extraction method comprises the following steps: in the step S1, the sieving mesh number is 200-300 meshes.
3. The efficient extraction method of moringa oleifera polysaccharide as claimed in claim 1, which is characterized in that: in the step S2, the temperature of hot water for ultrasonic extraction of moringa polysaccharide is controlled to be 50 ℃.
4. The efficient extraction method of moringa oleifera polysaccharide as claimed in claim 1, which is characterized in that: in the step S3, the extraction times are controlled to be 2 times, and the extraction rate of the moringa oleifera leaf polysaccharide reaches 13.30% after the single extraction time is controlled to be 30 min.
5. The efficient extraction method of moringa oleifera polysaccharide as claimed in claim 1, which is characterized in that: in the step S5, the temperature of the extracting solution is controlled to be 75 ℃, the time of single soaking extraction is controlled to be 180min, and the times of soaking extraction are controlled to be 4 times.
6. The efficient extraction method of moringa oleifera polysaccharide as claimed in claim 1, which is characterized in that: in the step S6, the temperature is controlled to be 80 ℃ when the three-factor three-level response surface method is adopted for extraction.
7. The efficient extraction method of moringa polysaccharide as claimed in claim 1, wherein the extraction method comprises the following steps: in the step S6, the formula of the extracting solution when the three-factor three-level response surface method is adopted for extraction is as follows: 150 to 400 portions of hot water, 100 to 200 portions of metaphosphoric acid-acetic acid solution of 0.03g/mL, 300 to 500 portions of 10 percent hydrochloric acid solution and 200 to 400 portions of 2 percent oxalic acid solution are added.
8. The efficient extraction method of moringa polysaccharide as claimed in claim 1, wherein the extraction method comprises the following steps: in the step S6, when a three-factor three-level response surface method is adopted for extraction, the extraction time is 1 hour, and the extraction rate of the moringa oleifera polysaccharide is 15.20 +/-0.30%.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105418787A (en) * | 2015-12-28 | 2016-03-23 | 哈尔滨工业大学 | Extraction method for polysaccharides in moringa oleifera seeds |
| CN107880142A (en) * | 2016-09-30 | 2018-04-06 | 华南农业大学 | A kind of preparation method and applications of moringa root Thick many candies |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105418787A (en) * | 2015-12-28 | 2016-03-23 | 哈尔滨工业大学 | Extraction method for polysaccharides in moringa oleifera seeds |
| CN107880142A (en) * | 2016-09-30 | 2018-04-06 | 华南农业大学 | A kind of preparation method and applications of moringa root Thick many candies |
Non-Patent Citations (1)
| Title |
|---|
| 彭凌;: "辣木多糖超声波辅助提取工艺条件优化研究" * |
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Application publication date: 20221227 |