CN105410280A - Preparation method of composite tea composed of extracts of dendrobe and gynostemma pentaphyllum - Google Patents
Preparation method of composite tea composed of extracts of dendrobe and gynostemma pentaphyllum Download PDFInfo
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Abstract
The invention discloses a preparation method of composite tea composed of extracts of dendrobe and gynostemma pentaphyllum. The composite tea is composed of, by mass, 50% of a dendrobe extract and 50% of a gynostemma pentaphyllum extract. The preparation method includes the following four steps: pre-treatment, extraction by means of ball milling and alcohol and alkali solution, supersonic-microwave extraction, and mixing and packaging. According to the method, the mechanical chemical-alcohol and alkali extraction technology and the supersonic-microwave extraction technology are employed, so that the preparation method not only overcomes the defects in single extraction process but also greatly reduces extraction time and extraction temperature. The preparation method is used for preparing the extract composite tea and has excellent development prospect.
Description
Technical field
The present invention relates to a kind of preparation method of stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea, belong to tea technical field.
Background technology
Tea is not only the diuresis of a kind of tool cardiac stimulant, the health beverages of the effect such as arousing brain, raising spirit, especially indispensable bavin in people's life, rice, oil, salt, sauce, vinegar, the one in tea.Though tea has a long history, but develop by leaps and bounds along with soda, fruit drink and mineral water, the status of tea little by little reduces.However, along with development and the people's living standard raising of society, more and more people focuses on health, and tea is a kind of health beverages meeting modern.This be due to modern tea be utilize pure natural plant to be deducted a percentage by scientific technology after add to inside drink tea, the existing good health value of tea is had again and certain drinks effect.
The effects such as the stem of noble dendrobium is a kind of medicinal plant, and its property slightly sweet flavor is micro-salty, flat, enters stomach, lung, kidney channel, has the beneficial stomach that promotes the production of body fluid, cleaning heat and expelling wind, the strong kidney of yin-nourishing, beneficial gas heat extraction, the long-pending essence of kidney tonifying, Appetizing spleen-tonifying, activating collaterals and eliminating stagnation, promoting blood circulation and removing blood stasis.Main pharmacodynamics composition in Dendrobium Sw is polysaccharide, alkaloid, glucosides, luxuriant and rich with fragrance class, bibenzyl and amino acid etc.The content of polysaccharide in dendrobium candidum is high, and has the effects such as good antitumor, anti-oxidant and develop immunitypty.In order to widen the range of application of the stem of noble dendrobium, people have carried out many-sided research to it, as Chinese patent CN1943450B discloses one " manufacture method of dendrobe compound tea ", its by percentage by weight be stem of noble dendrobium 15%-30%, the forming of glossy ganoderma 30%-40%, hawthorn 40%-50%, and through decocting, mix rock sugar syrup, filtration, sterilization, sterilization, bottling, can obtain having anti-ageing, eliminating pathogen to support vital qi function and the good dendrobe compound tea of mouthfeel.
Gynostemma pentaphylla, also known as seven leaf courages, is cucurbitaceae genus gynostemma.Its bitter cold in nature, return lung, spleen, kidney channel, have and replenish qi to invigorate the spleen, calm the nerves, hypotensive, clearing heat and detoxicating, the effects such as cough-relieving apophlegmatic.This is owing to containing the number of chemical compositions such as saponin, polysaccharide, flavone compound, organic acid and trace element in Herb Gynostemmae Pentaphylli extract, wherein more than 50 planting saponin, the same with panaxoside, belongs to tetracyclic triterpenes agate saponin.Chinese patent CN104721414A discloses one " a kind of gynostemma pentaphylla fat decreasing tea ", this tea is made up of gynostemma pentaphylla, hawthorn, ginkgo biloba p.e, rhizoma alismatis, cassia seed, lotus leaf, Radix Puerariae, prepared fleece flower root, the fruit of Chinese wolfberry, it mainly make use of the saponin of gynostemma pentaphylla, makes it have the health care of blood-fat-decreasing.
In order to give full play to effect of the stem of noble dendrobium and gynostemma pentaphylla, people have done further research to them, as CN101695328B discloses one " a kind of dendrobium tea ", this tea is by the stem of noble dendrobium 3 ~ 7 parts, gynostemma pentaphylla 2 ~ 3 parts, Mao Xu Cao 3 ~ 7 parts, tealeaves 3 ~ 7 parts, 3 ~ 5 parts, sugar is made, and it mainly overcomes the defect of the single effect of the stem of noble dendrobium.Although it can make each component play synergy, make it to have more various health-care to human body, and can body immunity be improved, but long-time high temperature water extraction has a negative impact to constituent structure and physiologically active in its preparation process, thus affect composition effect in tea, and then affect the effect of tea.Therefore, develop that a kind of extraction time is short and the compound tea preparation method that temperature is low is very important.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, the invention provides the short and preparation method of the stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea that temperature is low of a kind of extraction time.
The present invention for the taked technical scheme that solves the problem is:
A kind of stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea, it is made up of Herba Dendrobii extract and Herb Gynostemmae Pentaphylli extract, and described Herba Dendrobii extract and the mass percent of Herb Gynostemmae Pentaphylli extract are 50%:50%; Described Herba Dendrobii extract and Herb Gynostemmae Pentaphylli extract adopt composite algorithm to extract; Described composite algorithm extracts the extraction method extraction comprising mechanochemical reaction, alcohol alkaline process, ultrasonic method and microwave method and combine.
The preparation method of described stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea comprises following technique: pretreatment, the extraction of ball milling-ol alkali, ultrasonic-Microwave Extraction and hybrid packed four steps:
(1) pretreatment: a) stem of noble dendrobium and gynostemma pentaphylla to be cleaned respectively, sterilization, is ground into powder, and crosses 100 ~ 120 mesh sieves, and at 60 DEG C dry 10 ~ 20 minutes, for subsequent use; B) 5% sodium acid carbonate ethanolic solution of secure ph=12, for subsequent use;
(2) ball milling-ol alkali extracts: a) under normal temperature, first by dendrobe powder: 5% sodium acid carbonate ethanolic solution: zirconia ball=1:1:2 weight ratio adds ball grinder, soak 15 ~ 20 minutes, then ball grinder to be fixed on rotating speed be ball milling 20 ~ 30 minutes on the ball mill of 200 ~ 300 turns; B) take out the rear mixture of grinding, add distilled water and dissolve twice, first time adds dendrobe powder quality 8 times of distilled water, stirs 20 ~ 30 minutes, and second time adds dendrobe powder quality 4 times of distilled water, stirs 10 ~ 15 minutes, filters, merging filtrate; C) on centrifuge filtrate being placed in 6000 ~ 8000 turns centrifugal 10 ~ 20 minutes, supernatant is got, with watery hydrochloric acid adjust ph to 4 ~ 5, concentrated, dry, grind, obtain Herba Dendrobii extract powder; D) use the same method and prepare Herb Gynostemmae Pentaphylli extract;
(3) ultrasonic-Microwave Extraction: a) by Herba Dendrobii extract powder 100 weight portion in selecting step (2), add the distilled water of 2 ~ 6 times of weight, be 40 ~ 80kHz, microwave frequency 2400 ~ 2500MHz in supersonic frequency, extract twice at 40 ~ 60 DEG C, 10 ~ 15 minutes first times, second time 5 ~ 10 minutes, filter, merging filtrate, on the centrifuge again filtrate being placed in 6000 ~ 8000 turns centrifugal 10 ~ 15 minutes, again filter, concentrated, dry, obtain Herba Dendrobii extract powder, for subsequent use; B) use the same method and prepare Herb Gynostemmae Pentaphylli extract;
(4) hybrid packed:
The Herba Dendrobii extract powder first step (3) obtained and Herb Gynostemmae Pentaphylli extract powder respectively spray-dried, pulverized 400 ~ 500 mesh sieves; Then be that Herba Dendrobii extract and the Herb Gynostemmae Pentaphylli extract powder of 50%:50% fully mixes by mass percent, stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea can be obtained.
Present invention utilizes mechanochemistry-ol alkali extractive technique to extract, not only overcome the defect of single extracting method, and reduce extraction time, reduction Extracting temperature widely and improve harvest rate, thus reduce temperature to the stem of noble dendrobium and the impact of the effective-part of Gynostemma pentaphyllum structure and activity, and then their effects can be given full play in compound tea, increase utilization rate and the absorptivity of compound tea.The thinking that mechanochemistry-ol alkali extracts is: by the stem of noble dendrobium, gynostemma pentaphylla and the mixing of 5% sodium acid carbonate ethanolic solution ball milling, under the effect of ball milling, them are made each other chemical reaction to occur, form some easily water-soluble or compounds of alcohol, then through step Isolation and purification such as dissolving, filtrations, thus the active ingredient of the stem of noble dendrobium and gynostemma pentaphylla is obtained.In addition, the stem of noble dendrobium after ball milling and gynostemma pentaphylla, particle diameter diminishes, surface becomes large, increased activity, and active ingredient can discharge very soon, and the time decreased of extraction, harvest rate are improved.
In order to reduce extraction time further, improve harvest rate and purity, the present invention additionally uses ultrasonic-Microwave Extraction technology and extracts.This is because this extracting method is under the state of low-temperature atmosphere-pressure, makes full use of the high energy effect of ultrasonic cavitation and microwave, not only overcomes the deficiency of high temperature extraction and single extracting method, and saves extraction time.
The invention has the beneficial effects as follows:
1, adopt mechanochemistry-ol alkali extractive technique to extract, not only overcome the defect of single extracting method, and reduce extraction time, reduction Extracting temperature widely and improve harvest rate., also extract in conjunction with ultrasonic-Microwave Extraction technology meanwhile, reduce extraction time further, improve harvest rate and purity;
2, get with traditional water extraction, alcohol extracting follows the example of compared with extraction, not only reduce Extracting temperature and reduce extraction time, and reducing the consumption of organic solvent, and then reducing the residual quantity of organic solvent in extract, also reducing organic solvent pollution on the environment simultaneously;
3, Herba Dendrobii extract and Herb Gynostemmae Pentaphylli extract are as the raw material of compound tea, fully the stem of noble dendrobium promote the production of body fluid the replenishing qi to invigorate the spleen of effect of beneficial stomach, cleaning heat and expelling wind, the strong kidney of yin-nourishing etc. and gynostemma pentaphylla, calm the nerves, the effect such as hypotensive, clearing heat and detoxicating, cough-relieving apophlegmatic combines, so both overcame the defect of single tea, increase active ingredient dissolution rate again, improve the absorptivity of compound tea.
4, the stem of noble dendrobium after ball milling and gynostemma pentaphylla, particle diameter diminishes, surface becomes large, increased activity, and active ingredient can discharge very soon, and the time decreased of extraction, harvest rate are improved.
Detailed description of the invention
Embodiment 1
A preparation method for stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea, comprises the following steps:
(1) pretreatment: a) stem of noble dendrobium and gynostemma pentaphylla to be cleaned respectively, sterilization, is ground into powder, and crosses 115 mesh sieves, and at 60 DEG C dry 15 minutes, for subsequent use; B) 5% sodium acid carbonate ethanolic solution of secure ph=12, for subsequent use;
(2) ball milling-ol alkali extracts: a) under normal temperature, first by dendrobe powder: 5% sodium acid carbonate ethanolic solution: zirconia ball=1:1:2 weight ratio adds ball grinder, soak 18 minutes, then ball grinder to be fixed on rotating speed be ball milling 25 minutes on the ball mill of 250 turns; B) take out the rear mixture of grinding, add distilled water and dissolve twice, first time adds dendrobe powder quality 8 times of distilled water, stirs 25 minutes, and second time adds dendrobe powder quality 4 times of distilled water, stirs 12 minutes, filters, merging filtrate; C) on centrifuge filtrate being placed in 7000 turns centrifugal 15 minutes, supernatant is got, by watery hydrochloric acid adjust ph to 4.5, concentrated, dry, grind, obtain Herba Dendrobii extract powder; D) use the same method and prepare Herb Gynostemmae Pentaphylli extract;
(3) ultrasonic-Microwave Extraction: a) by Herba Dendrobii extract powder 100 weight portion in selecting step (2), add the distilled water of 4 times of weight, is 60kHz, microwave frequency 2450MHz in supersonic frequency, extracts twice at 50 DEG C, 12 minutes first times, second time 8 minutes, filters, merging filtrate, on the centrifuge again filtrate being placed in 7000 turns centrifugal 12 minutes, again filter, concentrated, dry, obtain Herba Dendrobii extract powder, for subsequent use; B) use the same method and prepare Herb Gynostemmae Pentaphylli extract;
(4) hybrid packed:
The Herba Dendrobii extract powder first step (3) obtained and Herb Gynostemmae Pentaphylli extract powder respectively spray-dried, pulverized 500 mesh sieves; Then be that Herba Dendrobii extract and the Herb Gynostemmae Pentaphylli extract powder of 50%:50% fully mixes by mass percent, stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea can be obtained.
Embodiment 2
A preparation method for stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea, comprises the following steps:
(1) pretreatment: a) stem of noble dendrobium and gynostemma pentaphylla to be cleaned respectively, sterilization, is ground into powder, and crosses 100 mesh sieves, and at 60 DEG C dry 10 minutes, for subsequent use; B) 5% sodium acid carbonate ethanolic solution of secure ph=12, for subsequent use;
(2) ball milling-ol alkali extracts: a) under normal temperature, first by dendrobe powder: 5% sodium acid carbonate ethanolic solution: zirconia ball=1:1:2 weight ratio adds ball grinder, soak 15 minutes, then ball grinder to be fixed on rotating speed be ball milling 20 minutes on the ball mill of 200 turns; B) take out the rear mixture of grinding, add distilled water and dissolve twice, first time adds dendrobe powder quality 8 times of distilled water, stirs 20 minutes, and second time adds dendrobe powder quality 4 times of distilled water, stirs 10 minutes, filters, merging filtrate; C) on centrifuge filtrate being placed in 6000 turns centrifugal 10 minutes, supernatant is got, by watery hydrochloric acid adjust ph to 4, concentrated, dry, grind, obtain Herba Dendrobii extract powder; D) use the same method and prepare Herb Gynostemmae Pentaphylli extract;
(3) ultrasonic-Microwave Extraction: a) by Herba Dendrobii extract powder 100 weight portion in selecting step (2), add the distilled water of 2 times of weight, is 40kHz, microwave frequency 2400MHz in supersonic frequency, extracts twice at 40 DEG C, 10 minutes first times, second time 5 minutes, filters, merging filtrate, on the centrifuge again filtrate being placed in 6000 turns centrifugal 10 minutes, again filter, concentrated, dry, obtain Herba Dendrobii extract powder, for subsequent use; B) use the same method and prepare Herb Gynostemmae Pentaphylli extract;
(4) hybrid packed:
The Herba Dendrobii extract powder first step (3) obtained and Herb Gynostemmae Pentaphylli extract powder respectively spray-dried, pulverized 400 mesh sieves; Then be that Herba Dendrobii extract and the Herb Gynostemmae Pentaphylli extract powder of 50%:50% fully mixes by mass percent, stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea can be obtained.
Embodiment 3
A preparation method for stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea, comprises the following steps:
(1) pretreatment: a) stem of noble dendrobium and gynostemma pentaphylla to be cleaned respectively, sterilization, is ground into powder, and crosses 120 mesh sieves, and at 60 DEG C dry 20 minutes, for subsequent use; B) 5% sodium acid carbonate ethanolic solution of secure ph=12, for subsequent use;
(2) ball milling-ol alkali extracts: a) under normal temperature, first by dendrobe powder: 5% sodium acid carbonate ethanolic solution: zirconia ball=1:1:2 weight ratio adds ball grinder, soak 20 minutes, then ball grinder to be fixed on rotating speed be ball milling 30 minutes on the ball mill of 300 turns; B) take out the rear mixture of grinding, add distilled water and dissolve twice, first time adds dendrobe powder quality 8 times of distilled water, stirs 30 minutes, and second time adds dendrobe powder quality 4 times of distilled water, stirs 15 minutes, filters, merging filtrate; C) on centrifuge filtrate being placed in 8000 turns centrifugal 20 minutes, supernatant is got, by watery hydrochloric acid adjust ph to 5, concentrated, dry, grind, obtain Herba Dendrobii extract powder; D) use the same method and prepare Herb Gynostemmae Pentaphylli extract;
(3) ultrasonic-Microwave Extraction: a) by Herba Dendrobii extract powder 100 weight portion in selecting step (2), add the distilled water of 6 times of weight, is 80kHz, microwave frequency 2500MHz in supersonic frequency, extracts twice at 60 DEG C, 15 minutes first times, second time 10 minutes, filters, merging filtrate, on the centrifuge again filtrate being placed in 8000 turns centrifugal 15 minutes, again filter, concentrated, dry, obtain Herba Dendrobii extract powder, for subsequent use; B) use the same method and prepare Herb Gynostemmae Pentaphylli extract;
(4) hybrid packed:
The Herba Dendrobii extract powder first step (3) obtained and Herb Gynostemmae Pentaphylli extract powder respectively spray-dried, pulverized 500 mesh sieves; Then be that Herba Dendrobii extract and the Herb Gynostemmae Pentaphylli extract powder of 50%:50% fully mixes by mass percent, stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea can be obtained.
Table 1 prior art and extraction time of the present invention and temperature comparisons
| Total preparation time (h) | High temperature total time (h) | Extracting temperature (DEG C) | |
| Prior art | 11.5～21 | 3.5～9 | Water seethes with excitement |
| Embodiment 1 | 4.48 | 0.67 | 50 |
| Embodiment 2 | 3.50 | 0.50 | 40 |
| Embodiment 3 | 5.50 | 0.83 | 60 |
As seen from the above table, Extracting temperature of the present invention, extraction time and production cycle are all low than prior art, this result describes the present invention and effectively can reduce extraction time and reduce Extracting temperature, simultaneously, extraction time is short, and Extracting temperature is low, can not destroy the stem of noble dendrobium and the effective-part of Gynostemma pentaphyllum, thus improve the harvest rate extracted, and then improve effect of compound tea.
The applicant is to the compound tea research experiment of the embodiment of the present invention 1 ~ 3, and concrete test method is as follows:
1, by body weight 15 ~ 20g and clinical symptoms are the mouse 60 of cold cough, heating (body temperature 38 ~ 39 DEG C), be divided into A group, B group and C group at random, often organize 20;
2, A group is taken compound tea, B group prepared by embodiment 1 and is taken compound tea prepared by embodiment 2 and C group and take compound tea prepared by embodiment 3;
3, instructions of taking: a day three times, each 10mg, takes 7 days;
4, temperature taking is carried out to the mouse taken 7 days, and record data.
Find after tested, mouse temperature has 13 lower than 38 DEG C: A group; B group has 11; C group has 12.If with mouse temperature lower than 38 DEG C for standard, then efficient 55%, the C group efficient 60% of efficient 65%, the B group of A group.As can be seen here, compound tea of the present invention is adopted to have heat-clearing effect.
Certainly, just the preferred embodiments of the disclosure is described in detail above, not limits practical range of the present invention with this, and all equivalence changes done according to principle of the present invention, structure and structure, all should be covered by protection scope of the present invention.
Claims (2)
1. a preparation method for stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea, is characterized in that: it comprises following technique: pretreatment, the extraction of ball milling-ol alkali, ultrasonic-Microwave Extraction and hybrid packed four steps:
(1) pretreatment: a) stem of noble dendrobium and gynostemma pentaphylla to be cleaned respectively, sterilization, is ground into powder, and crosses 100 ~ 120 mesh sieves, and at 60 DEG C dry 10 ~ 20 minutes, for subsequent use; B) 5% sodium acid carbonate ethanolic solution of secure ph=12, for subsequent use;
(2) ball milling-ol alkali extracts: a) under normal temperature, first by dendrobe powder: 5% sodium acid carbonate ethanolic solution: zirconia ball=1:1:2 weight ratio adds ball grinder, soak 15 ~ 20 minutes, then ball grinder to be fixed on rotating speed be ball milling 20 ~ 30 minutes on the ball mill of 200 ~ 300 turns; B) take out the rear mixture of grinding, add distilled water and dissolve twice, first time adds dendrobe powder quality 8 times of distilled water, stirs 20 ~ 30 minutes, and second time adds dendrobe powder quality 4 times of distilled water, stirs 10 ~ 15 minutes, filters, merging filtrate; C) on centrifuge filtrate being placed in 6000 ~ 8000 turns centrifugal 10 ~ 20 minutes, supernatant is got, with watery hydrochloric acid adjust ph to 4 ~ 5, concentrated, dry, grind, obtain Herba Dendrobii extract powder; D) use the same method and prepare Herb Gynostemmae Pentaphylli extract;
(3) ultrasonic-Microwave Extraction: a) by Herba Dendrobii extract powder 100 weight portion in selecting step (2), add the distilled water of 2 ~ 6 times of weight, be 40 ~ 80kHz, microwave frequency 2400 ~ 2500MHz in supersonic frequency, extract twice at 40 ~ 60 DEG C, 10 ~ 15 minutes first times, second time 5 ~ 10 minutes, filter, merging filtrate, on the centrifuge again filtrate being placed in 6000 ~ 8000 turns centrifugal 10 ~ 15 minutes, again filter, concentrated, dry, obtain Herba Dendrobii extract powder, for subsequent use; B) use the same method and prepare Herb Gynostemmae Pentaphylli extract;
(4) hybrid packed:
The Herba Dendrobii extract powder first step (3) obtained and Herb Gynostemmae Pentaphylli extract powder respectively spray-dried, pulverized 400 ~ 500 mesh sieves; Then be that Herba Dendrobii extract and the Herb Gynostemmae Pentaphylli extract powder of 50%:50% fully mixes by mass percent, stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea can be obtained.
2. the preparation method of stem of noble dendrobium Herb Gynostemmae Pentaphylli extract compound tea according to claim 1, is characterized in that: described ball milling rotating speed be 250 turns, Ball-milling Time is carry out under 25 minutes.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695328A (en) * | 2009-10-27 | 2010-04-21 | 白汉玉 | Dendrobium tea |
| CN102961412A (en) * | 2012-10-10 | 2013-03-13 | 中国科学院过程工程研究所 | Ball-milling coupling extraction integrated process for strengthening natural plant active ingredient extraction |
-
2015
- 2015-12-22 CN CN201510975631.XA patent/CN105410280A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695328A (en) * | 2009-10-27 | 2010-04-21 | 白汉玉 | Dendrobium tea |
| CN102961412A (en) * | 2012-10-10 | 2013-03-13 | 中国科学院过程工程研究所 | Ball-milling coupling extraction integrated process for strengthening natural plant active ingredient extraction |
Non-Patent Citations (1)
| Title |
|---|
| 郭力等主编: "《中药化学实验》", 31 July 2015, 中国医药科技出版社 * |
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Application publication date: 20160323 |