CN105393108A

CN105393108A - Quantum-dot immunochromatographic test strip detection system and use thereof

Info

Publication number: CN105393108A
Application number: CN201380076286.5A
Authority: CN
Inventors: 马义才; 顾敏; 马灵
Original assignee: Chengdu Lingyu Biotechnology Co ltd
Current assignee: Chengdu Lingyu Biotechnology Co ltd
Priority date: 2013-04-30
Filing date: 2013-04-30
Publication date: 2016-03-09
Also published as: WO2014176753A1

Abstract

A quantum-dot immunochromatographic detection system and a use thereof. The detection system comprises a test strip card (1) and a detection device (2), wherein the test strip card (1) comprises a card box (16) and a quantum-dot immunochromatographic test strip (15), and the card box (16) has an electronic tag (20), in which parameters, such as a standard operation curve of a detected object and the like, are stored; the detection device (2) comprises an optical system (3), a photoelectric detector (4), an analogue/digital converter (5), a data processing device (6), an electronic tag reading and writing module (10), a voice module (18), a power source (7) and an output display device (8); the detection system further comprises a wireless communication module (12) and a wireless network system (13) associated with the wireless communication module (12). The data processing device (6) calculates a characteristic frequency optical signal transmitted by a detection band(27) and a quality control band (28) of the test strip (15) in combination with a parameter sent by the electronic tag (20) to obtain a sample detection result, the detection result is displayed by the output display device (8), information about the detection result is prompted by the voice module (18), and the information about the detection result is sent to a remote server and/or a mobile terminal device (14) of a wireless network system (13) by the wireless communication module (12) for data management and information consultation feedback. The system has the characteristics of being easy and fast, being highly sensitive, having objective results, and having flexibility of use and so on when detecting a sample.

Description

Quantum dot immune chromatographs strip detecting system and its application

Technical field

The present invention relates to biomedical devices field, and in particular to a kind of quantum dot immune chromatography strip detecting system and its application.

Background technology

Sample multicomponent analysis has very important effect to medical diagnosis on disease, environmental monitoring, food security, Pharmaceutical Analysis, Micro biological Tests etc..In clinical tumor diagnosis, it need to often be realized by the related several tumor markerses of joint-detection to certain tumour Accurate Diagnosis.In environmental monitoring, often need to monitor the concentration index of a variety of insecticides and herbicide in natural water simultaneously with overall merit Environmental Degradation.Sample various ingredients content in mixed system is traditionally determined, one of which constituent content is only determined using parallel one pack system analytic approach, i.e. each analysis flow more, the multiple parallel execution flow finally obtains all required constituent contents.Long the time required to analysis, consumption reagent is more, and analysis throughput is low, and the amount of labour is big.Realize the simultaneously and rapidly quantitative analysis of sample various ingredients, need not be always particularly that people thirst for solving and unsolved problem for a long time by additional instruments equipment and reagent and independent of the live simultaneously and rapidly quantitative analysis of sample multicomponent that professional and technical personnel can be carried out.

The nano-quantum point of developed recently（quantum dots, QDs）Fluorescence radiation efficiency high, exciting line scope is wide, can " unitary is excited, polynary transmitting ", spectral line of emission narrow range and symmetrically, photobleaching speed is slow, fluorescence lifetime is long, and particle diameter is close with biomolecule, energy multifunction after surface modification, the characteristic wavelength fluorescence spectrum that different-grain diameter and the quantum azeotropic mixture of species are produced is not overlapped, and is highly suitable for sample multicomponent analysis.

Immunochromatography technique is simple, quick, and a few minutes are with regard to that can obtain testing result.But marked carry out sample detection using collaurum more than existing immunochromatography technique.Colloid gold test strip detection sample can only be qualitative, it is impossible to quantitative, can not realize sample multi-analyte immunoassay.

Unique advantage and immunochromatography technique of the invention according to QDs in terms of sample multicomponent analysis is simple, quick advantage, with reference to advanced manufacturing technology, a kind of quantum dot immune chromatography strip detecting system and its application are disclosed, with solve prior art still it is indeterminable " sample multicomponent scene Quantitative detection, remote quantitative detection and need not be by additional instruments equipment and reagent; ordinary person is with regard to that can carry out operation detection " the problem of, biomedical detection technique is set to come into community, move towards street, come into family, benefit the society, bring benefit to the people.

Present invention

First purpose of the present invention is to disclose a kind of quantum dot immune chromatography strip detecting system.

It is another object of the present invention to disclose application of the quantum dot immune chromatography strip detecting system in field of biological medicine.

Quantum dot immune chromatography strip detecting system of the present invention is achieved through the following technical solutions：

The quantum dot immune chromatographs strip detecting system, including test strip card 1 and detection means 2.Test strip card 1 includes cartridge 16 and the quantum dot immune that can be inserted into good time in cartridge 16 chromatography strip 15.The detection means 2 includes optical system 3, photoelectric detector 4, A/D converter 5, data processing equipment 6, electronic label read-write module 10, output display device 8, voice module 18, power supply 7, the button 9 positioned at the surface of detection means 2 and the standby multiple communication interfaces 31 for being available for connecting outside interim grafting stayed in detection means 2.The imaging signal end of optical system 3 is connected with photoelectric detector 4.The signal output part of photoelectric detector 4 is connected after amplifying through signal with the signal input part of A/D converter 5.The signal output part of A/D converter 5 is connected with the signal input part of data processing equipment 6.Data processing equipment 6 is also respectively with output display device 8, voice module 18, electronic label read-write module 10, power supply 7, the button 9 on the surface of detection means 2 and standby staying each communication interface 31 in detection means 2 to be connected.Power supply 7 is powered by data processing equipment 6 to optical system 3, photoelectric detector 4, A/D converter 5, output display device 8, voice module 18 and electronic label read-write module 10.Detection means 2 has a test strip card jack 33.Test strip card 1 is the separated type plug structure of detection means 2.Strip 15 is the separated type plug structure of test strip card 1.Test strip card 1 engages slot mark 19 at the test strip card jack 33 corresponding to detection means 2 provided with strip.The sample in strip is detected in the test strip card jack 33 for inserting detection means 2 after the insertion test strip card 1 of strip 15 in the lump together with test strip card 1.

One end of test strip card cartridge 16 forms strip slot mouthful 17 together with the upper box face opening at the cartridge end and inserts strip 15 to facilitate.Strip 15 in cartridge 16 includes sequentially overlapping the sample suction pad 21 being fixed on end liner 26, label pad 22, analyzing film 23, adsorptive pads 24.Analyzing film 23 has detection band 27 and quality control band 28.Detection band 27 is one or more.The upper box face of cartridge 16 is provided with detection window 29 at corresponding to the position of strip analyzing film 23.When strip 15 is inserted into test strip card cartridge 16, the detection correspondence of window 29 of cartridge 16 is located at the photoirradiated surface of the optical system 3 of detection means 2.Any place of cartridge 16 is provided with the electronic tag 20 of the detection information of strip 15 that is stored with.

The label pad 22 of strip 15 is coated with one or more by the quantum dot-labeled correlation molecule that target is detected analyte detection that is used in cartridge 16, and the quantum dot includes ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag₂S、CdS/PbS、CdS/Cd(0H)₂、CdS/HgS、CdS/HgS/CdS、ZnS/CdS、ZnS/CdS/ZnS、ZnS/HgS/ZnS/CdS、CdSe/CdS、CdSe/ZnS、CdSe/ZnSe、CdSe/CuSe、CdSe/HgTe、CdSe/HgSe、CdSe/HgSe/CdSe、CdTe/HgS、CdTe/HgTe、InAs/InP、InAs/CdSe、InAs/ZnSe、MgS、MgSe、MgTe、CaS、CaSe、CaTe、SrS、SrSe、SeTe、BaS、BaSe、BaTe、CdS:Mn、ZnS:Mn、CdS:Cu、ZnS:Cu、CdS:Tb、ZnS:The combination of any one or any several nano-particles in Tb, and by any one above-mentioned quantum dot is core, silica is shell core-shell type nano-complex particle.

The detection band 27 of strip 15 is coated with one or more another molecules related to the tested analyte detection of target in cartridge 16.

The quality control band 28 of strip 15 is coated with the Quality Control thing including secondary antibody in cartridge 16.

Electronic tag 20 in cartridge 16 includes but is not limited to RFID tag, the IC card chip of non contact angle measurement.Electronic tag 20 includes film adhesive type, encapsulation implanted, cassette, nail type or button-shaped etc. in the mounting means of cartridge 16.The strip detection that electronic tag 20 is stored includes the standard working curve or figure parameters, strip quality control band optical density reference monitor value with the quantitative sample checking matter concentration of batch quantum dot immunity-chromatography test strip 15 with information（OD_{Quality control band '}）, strip title, strip lot number, the strip effect phase, electronic tag password, clinical indices reference value, strip production firm information etc., and the information such as measurand identity information, tester's information, sample ID, sample number into spectrum, detection date, testing result can be read in.The checking matter standard working curve that electronic tag 20 is stored has diversified forms available, and it includes but is not limited to one of following curves：

（1）Checking matter standard items series concentration and OD_{Detection band}/OD_{Quality control band}Between corresponding relation curve；Or,

（2）Checking matter standard items series concentration and OD_{Detection band}/（OD_{Detection band}+OD_{Quality control band}）Between corresponding relation curve；Or,

（3）Checking matter standard items series concentration and OD_{Detection band}Between corresponding relation curve.

The OD_{Detection band}It is defined as the detection band OD value that checking matter standard items series concentration is measured, OD_{Quality control band}It is defined as the quality control band OD value that checking matter standard items series concentration is measured.

The structure composition of optical system 3 has kinds of schemes available, and it includes but is not limited to：

（1）It includes an illuminator and imaging system.Illuminator includes an excitation source 311.Fibre bundle 312, collimated illumination lens 313, dichronic mirror 314, front lens group 315 are followed successively by the output light path of the light source 311, until test strip card 1.Excitation source 311 includes LED or laser diode.Imaging system includes front lens group 315, dichronic mirror 314, optical filter 316, the rear microscope group 317 of common optical axis.The reflecting surface of dichronic mirror 314 and optical axis included angle are into 45o angles.Front lens group 315 and rear microscope group 317 use isolating construction.Illuminator light path is perpendicular in the forward part of dichronic mirror 314 and the optical axis of imaging system, the part after dichronic mirror 314 and imaging system common optical axis.Photoelectric detector 4 is located in the rear image planes of microscope group 317.The light that light source 311 is sent is divided into the at regular intervals and intensity identical laser beam of tool by fibre bundle 312, the laser beam is collimated as parallel beam incident to the surface of dichronic mirror 314 by collimated illumination lens 313, bleeding is excited to send characteristic frequency reflected fluorescent light to the quantum dot reaction signal thing of detection band 27 and quality control band 28 in the detection band 27 and quality control band 28 of the strip 15 of the object plane test strip card 1 of front lens group 315 by synchronous be irradiated to of front lens group 315 after being reflected through dichronic mirror 314, detection band 27 and the reflected fluorescent light of quality control band 28 are through same front lens group 315, through dichronic mirror 314, go out rear microscope group 317 after the elimination veiling glare of optical filter 316 and be converted to electric signal into detected in photoelectric detector 4.Or：

（2）It includes an excitation source 321, and incident photo-coupler 322, incident optical 323, fibre-optical probe 324, the output optical fiber 325, outgoing photo-coupler 326 are followed successively by the output light path of the excitation source 321, until photoelectric detector 4.Excitation source 321 includes LED or laser diode.The light that light source 321 is sent enters incident optical 323 through the incident beam splitting of photo-coupler 322, incident optical 323 is respectively radiated on the detection band 27 of the strip 15 of test strip card 1 and quality control band 28 through strip detection band fibre-optical probe and strip quality control band fibre-optical probe to excite the quantum dot reaction signal thing of strip detection band 27 and quality control band 28 to send characteristic frequency reflected fluorescent light, and reflected fluorescent light enters to be detected in photoelectric detector 4 after band fibre-optical probe and quality control band fibre-optical probe, corresponding the output optical fiber 325, outgoing photo-coupler 326 after testing respectively is converted to electric signal.Fibre-optical probe 324 is incident optical 323 using beam sharing structure, its center is closed, and periphery is the output optical fiber 325.

Photoelectric detector 4 has a variety of available objects, and it includes CCD, CMOS, photomultiplier, photodiode, phototriode.

Data processing equipment 6 is microprocessor, single-chip microcomputer or PC with corresponding data processing and control software.

The automatic information of read/write electronic tag 20 in a non contact fashion of electronic label read-write module 10.

The standby multiple communication interfaces 31 stayed of detection means 2 are USB interface or RS-232 interface, and it is used for tester and connects printer, external memory medium, host computer, keyboard or other instruments equipment according to outside the detection interim grafting of needs.

Output display device 8 is alphanumeric LCD liquid crystal displays, LED, touch display screen, audio unit or host computer etc..

Power supply 7 is external power supply or disconnectable battery.The battery includes lithium battery.

During sample detection, its one end interference of sample suction pad 21 of the strip 15 being inserted into test strip card cartridge 16 stretches out the openend of test strip card cartridge 16 to impregnate pipette samples.The light that optical system 3 is sent excites the quantum dot reaction signal thing of strip detection band 27 and quality control band 28 to send characteristic frequency reflected fluorescent light, the reflected fluorescence signal is received and converted into electric signal by photoelectric detector 4, electric signal after conversion is transferred to A/D converter 5 and is converted to data signal and is transferred to data processing equipment 6 after amplifying through signal, the characteristic frequency fluorescence signal that 6 pairs of transmission of data processing equipment come carries out automatic identification, and combine the checking matter standard working curve for being sent in test strip card electronic tag 20 or figure parameters progress checking matter concentration calculating and technical Analysis that the transmission of electronic label read-write module 10 comes, result is transferred to output display device 8, the voice message testing result information of voice module 18.

Quantum dot immune chromatography strip detecting system of the present invention may also include a wireless communication module 12 being connected with the data processing equipment 6 of the system and a Radio Network System 13,14 being communicated with the signal transmitting terminal of wireless communication module 12, including remote server 14 or/and other mobile terminal devices 14.Communication modes between the wireless communication module 12 and the Radio Network System 13,14 include but is not limited to Wifi, bluetooth, short message, wechat, mobile phone etc..Power supply 7 is also powered to wireless communication module 12.Data processing equipment 6 transmits the characteristic frequency fluorescence signal come to detection band 27 and quality control band 28 and carries out automatic identification, and combine the checking matter standard working curve for being sent in test strip card electronic tag 20 or figure parameters progress checking matter concentration calculating and technical Analysis that the transmission of electronic label read-write module 10 comes, result is transferred to output display device 8, the voice message testing result information of voice module 18, testing result and its relevant information are sent to Radio Network System 13 by wireless communication module 12, 14 remote server 14 or/and mobile terminal device 14 carries out data management and fed back with information consultation.

The data processing equipment 6 of quantum dot immune chromatography strip detecting system of the present invention can also integrate the data processing equipment 6 described in composition with electronic label read-write module 10, and the described data processing equipment 6 transmits the characteristic frequency fluorescence signal come to detection band 27 and quality control band 28 and carries out automatic identification and combine the checking matter standard working curve sent from test strip card electronic tag 20 or figure parameters progress checking matter concentration calculating and technical Analysis.

The electronic tag 20 of quantum dot immune chromatography strip detecting system of the present invention can also be arranged on any place of strip 15 in cartridge 16.

Can also be overlapped with one piece between the sample suction pad 21 and label pad 22 of strip 15 in the cartridge 16 of quantum dot immune chromatography strip detecting system of the present invention is used for the filter sample pad 11 of sample separating treatment.The filter sample pad 11 includes hemofiltration film.

The tail end of the adsorptive pads 24 of strip 15 can also be overlapped with a strip reaction end indicating label 25 in the cartridge 16 of quantum dot immune chromatography strip detecting system of the present invention.The strip reaction end indicating label 25 includes but is not limited to the accurate pH test paper that color change interval is 5.0-9.0.The test strip card cartridge 16 is provided with strip reaction end observation window 30 at the position corresponding to strip reaction end indicating label 25.

The detection means 2 of quantum dot immune of the present invention chromatography strip detecting system be on the whole portable instrument, in/large-scale instrument or be and wireless telecommunications product with corresponding transmitting and receive information function combination including and mobile phone, tablet personal computer, personal digital assistant, mobile terminal device or set of computers in the combination for selecting product.

Strip 15 in the test strip card 1 of quantum dot immune chromatography strip detecting system of the present invention is disposable strip；It is the use product matched with batch strip for the electronic tag 20 installed in the cartridge 16 and cartridge 16 of the test strip card 1 that inserts strip 15 or in strip 15；The detection means 2 is to continue universal product.

The preparation method of strip of the present invention comprises the following steps：

（1）It is prepared by strip component

1. sample suction pad 21: Make sample suction pad material from cellulose membrane, be cut into the film block with certain specification, the film block is put into sample suction pad treatment fluid（PH=7.2,0.03mol/L phosphate buffer+5%BSA+0.1%Tween 20）Middle immersion, takes out, and drying makes its abundant drying for standby.

2. sample pad 11 is filtered：Sample filtering film is selected by filter sizes, certain specification film block, drying for standby is cut into.

3. label pad 22：Marked cushion material from glass fibre membrane, be cut into the film block with certain specification, be detected the conjugate solution of analyte detection correlation molecule with target plus quantum dot on the film block, desciccator diaphragm block is standby.

4. analyzing film 23： Performed an analysis membrane material from cellulose membrane, it is cut into the film block with certain specification, the related another kind special molecular of analyte detection is detected as detection band 27 and specking from upper specking target respectively separated by a distance being included the Quality Control thing of secondary antibody as quality control band 28, desciccator diaphragm block is standby from film block base by lower.

5. adsorptive pads 24：From the cellulose membrane with strong water sorption and it is cut into the film block with certain specification, abundant drying for standby.

6. strip reaction end indicating label 25： From the accurate pH test paper that color change interval is 5.0-9.0, the film block with certain specification, abundant drying for standby are cut into.

（2）Strip is assembled

Preferably, by the above-mentioned strip component prepared, by sample suction pad 21, label pad 22, analyzing film 23, adsorptive pads 24, sequentially mutually overlap joint is pasted on plastics end liner 26, and cut into the strip of certain specification, drying for standby.

It is another preferably, by the above-mentioned strip component prepared, by sample suction pad 21, filter sample pad 11, label pad 22, analyzing film 23, adsorptive pads 24, sequentially mutually overlap joint is pasted on plastics end liner 26, and cut into the strip of certain specification, drying for standby.

More preferably, by the above-mentioned strip component prepared, by sample suction pad 21, label pad 22, analyzing film 23, adsorptive pads 24, strip reaction end indicating label 25, sequentially mutually overlap joint is pasted on plastics end liner 26, and cut into the strip of certain specification, drying for standby.

Further preferably, by the above-mentioned strip component prepared, by sample suction pad 21, filter sample pad 11, label pad 22, analyzing film 23, adsorptive pads 24, strip reaction end indicating label 25, sequentially mutually overlap joint is pasted on plastics end liner 26, and cut into the strip of certain specification, drying for standby.

Strip chromatography reaction is a dynamic process.The tested analyte detection strips of difference and different batches strip, the difference such as its preparation technology, material selection, detection environment, its detection sensitivity, stability, specificity etc. there may be difference, so as to influence whether the accurate measure of checking matter concentration（For example, make that cellulose membrane source used in strip is different, membrane filtration hole is of different sizes, strip thickness is not equal there is a possibility that strip reactant will not be moved ahead with identical siphon bleeding speed, reactant is it could even be possible in strip bleeding way before being trapped in strip detection band 27, quality control band 28；Field condition detects and indoor detection, the difference such as temperature, humidity, it is also possible to has influence on bleeding of the strip reactant in strip and moves ahead speed, make its incomplete same, etc.）.The present invention can solve the above problems very well, improve accuracy and the flexibility of sample detection：The present invention uses electronic tag 20 to store same batch strip checking matter respective standard working curve or figure parameters with quantitative with batch strip checking matter concentration, standard working curve or figure parameters on electronic tag 20 can before the product export of test strip card 1 according between strip batch and batch in difference calibrated, this meets national strip " batch criticize examine " specification；Electronic tag 20 of the present invention is also stored with strip optical density reference monitor value（OD_{Quality control band '}）, when sample is actually detected, it can point out whether strip course of reaction is set up, and judge whether testing result is authentic and valid（If strip reaction failure, the OD obtained in detection_{Quality control band}Meeting and the corresponding strip optical density reference monitor value read from electronic tag 20（OD_{Quality control band '}）Produce very big statistical errors）；The tail end of strip 15 of the present invention is settable strip reaction end indicating label 25, can point out whether siphon bleeding chromatography of the strip reactant in strip be abundant, whether reaction reaches home；Strip 15 of the present invention is provided with quality control band 28, and the quality control band 28 plays detection control.

The preparation method for the checking matter standard working curve that electronic tag 20 of the present invention is stored comprises the following steps：（1）Prepare checking matter standard items series concentration.（2）Detection obtains the corresponding OD of checking matter standard items series concentration_{Detection band}And OD_{Quality control band}, and calculate acquisition OD_{Detection band}/OD_{Quality control band}Ratio or OD_{Detection band}/（OD_{Detection band}+OD_{Quality control band}）Ratio.（3）X-axis is made with standard items series concentration, test result is with OD_{Detection band}/OD_{Quality control band}Ratio makees Y-axis, draws standard working curve；Or X-axis is made with standard items series concentration, test result is with OD_{Detection band}/（OD_{Detection band}+OD_{Quality control band}）Ratio makees Y-axis, draws standard working curve；Or X-axis is made with standard items series concentration, test result is with OD_{Detection band}Value makees Y-axis, draws standard working curve.（4）Standard of compiling working curve software.（5）The strip quality control band OD value obtained when standard items are tested is used as strip quality control band optical density reference monitor value（That is OD_{Quality control band '}）；By standard working curve software and OD_{Quality control band '}It is stored in the lump on electronic tag 20.

The spectral signal for the quantum dot reaction signal thing that present invention detection sample chromatographs strip detection band 27 based on quantum dot immune is proportionate with testing sample concentration.Checking matter concentration is bigger in sample, and the characteristic frequency reflected fluorescence signal that the quantum dot reaction signal thing of bleeding to strip detection band 27 is sent is stronger.Conversely, checking matter concentration is smaller in sample, the characteristic frequency reflected fluorescence signal that the quantum dot reaction signal thing in strip detection band 27 is sent is weaker.According to this relation, the present invention will insert the interference external part of strip in test strip card 1（That is the end of sample suction pad 21）Impregnated sample, the fluid sample of the checking matter containing target is under the pulling positioned at the adsorptive pads 24 of the strip other end, using the capillary siphoning effect of strip miillpore filter slowly to strip rear end bleeding, when there is target checking matter molecule in fluid sample（Such as antigen or antibody）When, they just be coated in the glass fibre membrane label pad 22 in the preceding stage casing of strip it is corresponding by quantum dot-labeled good immune molecule combine and together with to strip rear end bleeding to the detection band 27 of strip analyzing film 23, another particular target checking matter correlated response molecule with being coated on detection band 27（Such as another specific antibody or antigen of respective objects checking matter molecule）Combine to form detection band quantum dot reaction signal thing and dam and be enriched in detection band 27.Remaining a part of quantum dot immune mark molecule continues to move ahead bleeding to strip quality control band 28, the Quality Control thing with being coated on quality control band 28（Such as secondary antibody）Quality control band quantum dot reaction signal thing is combined to form, and dams and is enriched on quality control band 28.The excitation source 311 of optical system 3,321 light sent send characteristic frequency fluorescence signal after exciting the quantum dot reaction signal thing of strip detection band 27 and quality control band 28, fluorescence signal is received by photoelectric detector 4 and is converted into respective strengths electric signal, and the electric signal after conversion is transferred to A/D converter 5 and is converted to data signal and is transferred to data processing equipment 6 after amplifying through signal is stored and data processing.Data processing equipment 6 transmits the characteristic frequency fluorescence signal come to detection band 27 and quality control band 28 and carries out automatic identification, obtains the OD value of checking matter detection band 27（OD_{Detection band}）With the OD value of quality control band 28（OD_{Quality control band}）And calculating obtains OD_{Detection band}/OD_{Quality control band}Ratio or OD_{Detection band}/（OD_{Detection band}+OD_{Quality control band}）Ratio, the checking matter standard working curve or figure parameters stored simultaneously by the automatic electronic tag 20 of reading test strips card 1 of electronic label read-write module 10, calculated with reference to checking matter standard working curve or figure parameters and obtain checking matter concentration, output display device 8 shows testing result and information, the voice message testing result information of voice module 18, the remote server 14 or/and other mobile terminal devices 14 that testing result and its relevant information are sent to Radio Network System 13,14 by wireless communication module 12 carry out data management and fed back with information consultation.

Sample of the present invention is included from clinical or blood, body fluid, urine, saliva, genital secretion or other liquid samples of non-clinical or thick sample, wherein, sample of the clinical sample including infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease, sample of the non-clinical sample including food inspection, environment pollution detection, pesticide residue determination, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection.The present invention is quantitatively detected simultaneously to the target checking matter one-component or multicomponent in above-mentioned sample.

The detection application method of quantum dot immune chromatography strip detecting system of the present invention is realized by following step：

（1）Start switches on power；

（2）Strip 15 is inserted in test strip card 1；The test strip card 1 for inserting strip is inserted into the test strip card jack 33 of the detection means 2 of the strip detecting system again；

（3）Strip sample suction end impregnated sample；

（4）Pen-down detection key, the quantum dot immune chromatography strip detecting system starts sample detection.After detection terminates, output display device 8 shows strip testing result information, the voice message testing result information of voice module 18；

（5）Send key is pressed, the remote server 14 or/and mobile terminal device 14 that testing result and information are sent to Radio Network System 13,14 carry out data management and information consultation feedback.

Beneficial effect of the present invention：

Compared with prior art, beneficial effect of the present invention mainly has：

（1）Once inhale sample energy simultaneous quantitative joint inspection sample multicomponent.Quantum dot fluorescence luminous efficiency is high, and exciting line scope is wide, and spectral line of emission narrow range, fluorescence lifetime is long, and Stokes displacements are big, and particle diameter is close with biomolecule, can multifunction after surface modification.The quantum dot of different-grain diameter, species and structure can produce different characteristic wavelength and continue fluorescence peak spectrum, and the characteristic wavelength fluorescence peak spectrum that quantum azeotropic mixture is produced is not overlapped.Checking matter respective reaction molecule is marked respectively using different quantum dots, is mixed thing and is coated in immune layer analysis strip and reacted with testing sample, by determining strip specific fluorescent signal, a few minutes interior energy quick and precisely measures sample multicomponent concentration.The strip of system of the present invention not only single detection band can synchronously quantify joint inspection sample multicomponent, when strip set a plurality of detection band when, the system then can bigger flux realize sample component synchronously quantify joint inspection.

（2）Joint inspection sample is quick, with regard to that can obtain testing result in a few minutes.

（3）Amount of samples is few, and several microlitres to tens microlitres can just meet detection needs.

（4）Sample need not be pre-processed.Can be set between the sample suction pad 21 and label pad 22 of the quantum dot immune chromatography strip of the system has the filter sample pad 11 that can be automatically separated processing sample.For example, measuring samples are blood sample, filter sample pad 11 may filter that haemocyte and only allow serum to ooze filtering sample pad 11, can just be detected with whole blood, the deficiency that could be detected after serum must be separated with instrument and equipments such as centrifuges by overcoming prior art detection blood sample.

（5）The final product form of strip detecting system of the present invention can be big-and-middle-sized detecting instrument, be used for social each professional institution's detection（Finished product its data processing equipment 6 under such a situation can use PC）；Can also be small portable detector, for by family, supermarket, street, sick bed and people's daily life scene, detection is used at once（Finished product its data processing equipment 6 under such a situation can use microprocessor）.

（6）Simple to operate, Quality Control is convenient, need not be in addition by instrument and equipment and reagent, and ordinary person can be achieved with sample multicomponent Quantitative detection, and overcoming prior art by professional and technical personnel and must need the conditions such as laboratory to carry out the deficiency operated.During present invention detection sample, strip 15 need to only be inserted test strip card 1 by tester, and the test strip card 1 for inserting strip is inserted into the test strip card jack 33 of detection means 2 again, and the interference external part of strip is impregnated into inspection product, and a few minutes just can accurately obtain testing result.

（7）Testing result information is standby to be provided in 3 kinds of modes：1. output display device 8 shows testing result information；2. the voice message testing result information of voice module 18（To facilitate the poor operator of eyesight such as the elderly's energy ear to listen testing result）；3. testing result information can carry out remote management and feedback of the information by mobile Internet（Validity and its meaning of testing result etc. can be seeked advice from etc. in good time including operator）.

（8）Strip 15 in test strip card 1 is disposable strip；The electronic tag 20 installed in test strip card cartridge 16 and cartridge 16 for inserting disposable test strips 15 or in strip 15 is the use product matched with batch strip；The detection means 2 is to continue universal product.During different sample detections, it is only necessary to change corresponding strip and sample Quantitative detection can be achieved in corresponding supporting test strip card cartridge 16.Detection means 2 of the present invention is had（For lasting universal product）Tester user, it is only necessary to purchase needed for strip 15（For disposable product）With the test strip card cartridge 16 matched with strip（The electronic tag 20 matched with same batch strip is installed thereon, electronic tag 20 is stored with the standard working curve or figure parameters of the quantitative sample checking matter concentration of same batch strip, is same batch strip matching product）, can be achieved with detecting needs.Strip 15 and test strip card cartridge 16 are cheap, and common tester with can bear per family.

（9）Testing result information can carry out remote management and advisory feedback by mobile Internet.Strip detecting system of the present invention can built-in wireless communication module 12, the wireless communication module 12 is connected with data processing equipment 6, and its signal transmitting terminal and Radio Network System 13,14 communicate.The remote server 14 or/and the grade of other mobile terminal devices 14 that the testing result and its relevant information that the analysis of data processing equipment 6 is obtained can be transmitted to Radio Network System 13,14 by wireless communication module 12 carry out corresponding data management and information consultation feedback.

（10）Dynamic monitoring can be carried out to strip chromatography course of reaction.Electronic tag 20 of the present invention is stored with strip optical density reference monitor value（OD_{Quality control band '}）, during strip reaction failure, the OD obtained in detection_{Quality control band}The corresponding strip optical density reference monitor value come is stored and read to electronic tag 20（OD_{Quality control band '}）Very big statistical errors can be produced；The tail end of strip 15 of the present invention is settable strip reaction end indicating label 25, can supervise and show whether siphon bleeding chromatography of the strip reactant in strip be abundant, and whether reaction reaches home.

（11）Detect that quality is controlled using many special quality control measure synchronous precisions：1. quality control band 28 is set in strip 15, and its optical density intensity plays detection control.2. electronic tag 20 stores corresponding checking matter standard working curve or figure parameters, for the corresponding checking matter concentration of accurate quantification.3. electronic tag 20 stores same batch strip quality control band optical density reference monitor value（OD_{Quality control band '}）, for monitoring whether strip reaction in sample detection sets up, whether testing result is effective.4. strip reaction end indicating label 25 is set in strip 15, and whether to point out siphon bleeding chromatography of the strip reactant in strip abundant, whether reaction reaches home.

（12）Sample detection embodies personalized.The detection means 2 of the strip detecting system is standby to leave multiple USB interfaces or RS-232 interface for being available for connecting printer, external memory medium, host computer, keyboard or other instruments equipment outside interim grafting, is taken with facilitating tester user to access, print or move testing result information in good time.

（13）It is extensive that the strip detecting system is applicable sample.The strip is disposable strip；The electronic tag 20 installed in test strip card cartridge 16 and cartridge 16 for inserting strip 15 or in strip 15 is the use product matched with batch strip；Detection means 2 is to continue universal product.Tester can be according to different detection needs, sample polycomponent quantitative analysis needed for selecting the quantum dot strip for changing respective classes just can quickly realize.Measuring samples can be the blood from clinical or non-clinical（Including whole blood, serum, blood plasma）, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample.Clinical sample includes the samples such as infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease；Non-clinical sample includes the samples such as food inspection, environment pollution detection, residues of pesticides analyte detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection.

Brief description of the drawings

Scheme l：Detection means of the present invention is not required to carry out the surface structure block diagram of the strip detecting system of remote data management and feedback of the information using mobile phone pattern, testing result.

Fig. 2：Detection means of the present invention is not required to carry out the internal structure block diagram of the strip detecting system of remote data management and feedback of the information using mobile phone pattern, testing result.Wherein, it is one to show strip detection band.

Fig. 3：Detection means of the present invention is not required to carry out the internal structure block diagram of the strip detecting system of remote data management and feedback of the information using mobile phone pattern, testing result.Wherein, it is two to show strip detection band.

Fig. 4：Detection means of the present invention is not required to carry out the internal structure block diagram of the strip detecting system of remote data management and feedback of the information using mobile phone pattern, testing result.Wherein show：Strip detection band is one；The integrated electronic label model 10 of data processing equipment 6.

Fig. 5：Detection means of the present invention can carry out the surface structure block diagram of the strip detecting system of remote data management and information consultation feedback using mobile phone pattern, testing result.

Fig. 6：Detection means of the present invention can carry out the internal structure block diagram of the detection means of the strip detecting system of remote data management and feedback of the information using mobile phone pattern, testing result.Wherein, it is one to show strip detection band.

Fig. 7：Detection means of the present invention can carry out the internal structure block diagram of the detection means of the strip detecting system of remote data management and feedback of the information using mobile phone pattern, testing result.Wherein, it is two to show strip detection band.

Fig. 8：Detection means of the present invention can carry out the internal structure block diagram of the detection means of the strip detecting system of remote data management and feedback of the information using mobile phone pattern, testing result.Wherein show：Strip detection band is one；The integrated electronic label model 10 of data processing equipment 6.

Fig. 9：The structured flowchart of the optical system preferred version one of strip detecting system of the present invention, wherein, it is one to show strip detection band.

Figure 10：The structured flowchart of the optical system preferred version two of strip detecting system of the present invention, wherein, it is one to show strip detection band.

Figure 11：Strip side block diagram.Wherein show：Strip component includes sample suction pad 21, label pad 22, analyzing film 23, adsorptive pads 24；Detection band 27 is one.

Figure 12：Strip side block diagram.Wherein show：Strip component includes sample suction pad 21, label pad 22, analyzing film 23, adsorptive pads 24；Detection band 27 is a plurality of.

Figure 13：Test strip card overlooking structure figure.Wherein, it is one to show strip detection band.

Figure 14：Test strip card overlooking structure figure.Wherein, it is a plurality of to show strip detection band.

Figure 15：Strip side block diagram.Wherein show：Strip component includes sample suction pad 21, filter sample pad 11, label pad 22, analyzing film 23, adsorptive pads 24；Detection band 27 is one.

Figure 16：Strip side block diagram.Wherein show：Strip component includes sample suction pad 21, label pad 22, analyzing film 23, adsorptive pads 24, strip reaction end indicating label 25；Detection band 27 is one.

Figure 17：Test strip card overlooking structure figure.Wherein, show that test strip card cartridge 16 is provided with strip reaction end observation window 30 at the position corresponding to strip reaction end indicating label 25.

Figure 18：Strip side block diagram.Wherein show：Strip component includes sample suction pad 21, filter sample pad 11, label pad 22, analyzing film 23, adsorptive pads 24, strip reaction end indicating label 25；Detection band 27 is one.

Figure 19：Tumor markers AFP, CEA, PSA examination criteria working curve.

Figure 20：Quantum dot-labeled AFP, CEA, PSA fluorescent spectrum curve.

Figure 21：Hepatitis B virus surface antigen（HBsAg）Examination criteria working curve.

Figure 22：The quantum dot-labeled HBsAg curves of spectrum.

Figure 23：The plan structure block diagram of test strip card another application scheme.

Sequence number is expressed as follows：1 test strip card, 2 detection means, 3 optical systems, 4 photoelectric detectors, 5 A/D converter, 6 data processing equipments, 7 power supplys, 8 output display devices, 9 buttons, 10 electronic label read-write modules, 11 filter sample pads, 12 wireless communication modules, 13 Radio Network Systems, the remote server or mobile terminal device of 14 Radio Network Systems, 15 strips, 16 test strip card cartridges, 17 strip slots mouthful, 18 voice modules, 19 strips engage slot mark, 20 electronic tags, 21 sample suction pads, 22 label pads, 23 analyzing films, 24 adsorptive pads, 25 strip reaction end indicating labels, 26 end liners, 27 detection bands, 28 quality control bands, 29 detection windows, 30 strip reaction end observation windows, the 31 standby multiple communication interfaces stayed, 32 wells, 33 test strip card jacks, 311 excitation sources, 312 fibre bundles, 313 collimated illumination lens, 314 dichronic mirrors, 315 front lens groups, 316 optical filters, microscope group after 317, 321 excitation sources, 322 incident photo-couplers, 323 incident opticals, 324 fibre-optical probes, 325 the output optical fibers, 326 outgoing photo-couplers

Embodiment

Embodiment 1

The present embodiment is that its detection means 2 of quantum dot strip detecting system of the present invention need not carry out a kind of preferred technique scheme of remote data management and feedback of the information using mobile phone pattern, testing result.1-4,9-18 illustrate technical scheme described in the present embodiment below in conjunction with the accompanying drawings.

Fig. 1-4 is the structured flowchart of quantum dot strip detecting system technical scheme described in the present embodiment.Wherein, Fig. 1 is the system appearance structured flowchart.Fig. 2-4 is the internal system structured flowchart.

In Fig. 1 combinations Fig. 2-4, the quantum dot immune chromatographs strip detecting system, including test strip card 1 and detection means 2.Test strip card 1 includes cartridge 16 and the quantum dot immune that can be inserted into good time in cartridge 16 chromatography strip 15.Detection means 2 includes optical system 3, photoelectric detector 4, A/D converter 5, data processing equipment 6, electronic label read-write module 10, output display device 8, voice module 18, power supply 7, the button 9 positioned at the surface of detection means 2 and the standby multiple communication interfaces 31 for being available for connecting outside interim grafting stayed in detection means 2.The imaging signal end of optical system 3 is connected with photoelectric detector 4.The signal output part of photoelectric detector 4 is connected after amplifying through signal with the signal input part of A/D converter 5.The signal output part of A/D converter 5 is connected with the signal input part of data processing equipment 6.Data processing equipment 6 is also respectively with output display device 8, voice module 18, electronic label read-write module 10, power supply 7, the button 9 on the surface of detection means 2 and standby staying each communication interface 31 in detection means 2 to be connected.Power supply 7 is powered by data processing equipment 6 to optical system 3, photoelectric detector 4, A/D converter 5, output display device 8, voice module 18 and electronic label read-write module 10.There is a test strip card jack 33 front end of detection means 2.Test strip card 1 is the separated type plug structure of detection means 2.Strip 15 is the separated type plug structure of test strip card 1.Test strip card 1 engages slot mark 19 at the test strip card jack 33 corresponding to detection means 2 provided with strip.The sample in strip is detected in the test strip card jack 33 for inserting detection means 2 after the insertion test strip card 1 of strip 15 in the lump together with test strip card 1.

One end of test strip card cartridge 16 forms strip slot mouthful 17 together with the upper box face opening at the cartridge end and inserts strip 15 to facilitate.The side view structure of the strip 15 of cartridge 16 shown in Figure 11,12,15,16,18.Wherein, strip 15 shown in Figure 11-12 includes sequentially overlapping the sample suction pad 21 being fixed on end liner 26, label pad 22, analyzing film 23, adsorptive pads 24.Strip 15 shown in Figure 15 includes sequentially overlapping the sample suction pad 21 being fixed on end liner 26, filter sample pad 11, label pad 22, analyzing film 23, adsorptive pads 24.Strip 15 shown in Figure 16 includes sequentially overlapping the sample suction pad 21 being fixed on end liner 26, label pad 22, analyzing film 23, adsorptive pads 24, strip reaction end indicating label 25.Strip 15 shown in Figure 18 includes sequentially overlapping the sample suction pad 21 being fixed on end liner 26, filter sample pad 11, label pad 22, analyzing film 23, adsorptive pads 24, strip reaction end indicating label 25.The analyzing film 23 of the strip 15 has detection band 27 and quality control band 28, and detection band 27 is one or more（Fig. 2,4,9-11,13, the detection band 27 of the analyzing film 23 of strip 15 shown in 15-18 are one；The detection band 27 of the analyzing film 23 of strip 15 shown in Fig. 3,12,14 is a plurality of）.The plan structure of test strip card 1 shown in Figure 13,14,17.The upper box face of test strip card cartridge 16 is provided with detection window 29 at corresponding to the position of strip analyzing film 23.Cartridge 16 is provided with strip reaction end observation window 30 at the position corresponding to strip reaction end indicating label 25（Shown in Figure 17）.Any place of cartridge 16 is provided with the electronic tag 20 of the detection information of strip 15 that is stored with.When strip 15 is inserted into test strip card cartridge 16, the detection correspondence of window 29 of cartridge 16 is located at the photoirradiated surface of the optical system 3 of detection means 2.

The structure composition of optical system 3 has kinds of schemes available, and it includes but is not limited to scheme described in scheme described in Fig. 9 or Figure 10.

In Fig. 9 schemes, the optical system 3 includes illuminator and imaging system.Wherein illuminator includes an excitation source 311.Fibre bundle 312, collimated illumination lens 313, dichronic mirror 314, front lens group 315 are followed successively by the output light path of the light source 311, until test strip card 1.Excitation source 311 includes LED or laser diode.Wherein imaging system includes front lens group 315, dichronic mirror 314, optical filter 316, the rear microscope group 317 of common optical axis.The reflecting surface of dichronic mirror 314 and optical axis included angle are into 45o angles.Front lens group 315 and rear microscope group 317 use isolating construction.Illuminator light path is perpendicular in the forward part of dichronic mirror 314 and the optical axis of imaging system, the part after dichronic mirror 314 and imaging system common optical axis.Photoelectric detector 4 is located in the rear image planes of microscope group 317.The light that light source 311 is sent is divided into the at regular intervals and intensity identical laser beam of tool by fibre bundle 312, the laser beam is collimated as parallel beam incident to the surface of dichronic mirror 314 by collimated illumination lens 313, bleeding is excited to send characteristic frequency reflected fluorescent light to the quantum dot reaction signal thing of detection band 27 and quality control band 28 in the detection band 27 and quality control band 28 of the strip 15 of the object plane test strip card 1 of front lens group 315 by synchronous be irradiated to of front lens group 315 after being reflected through dichronic mirror 314, detection band 27 and the reflected fluorescent light of quality control band 28 are through same front lens group 315, through dichronic mirror 314, go out rear microscope group 317 after the elimination veiling glare of optical filter 316 and be converted to electric signal into detected in photoelectric detector 4.

In Figure 10 schemes, the optical system 3 includes an excitation source 321, incident photo-coupler 322, incident optical 323, fibre-optical probe 324, the output optical fiber 325, outgoing photo-coupler 326 are followed successively by the output light path of the excitation source 321, until photoelectric detector 4.Excitation source 321 includes LED or laser diode.The light that light source 321 is sent enters incident optical 323 through the incident beam splitting of photo-coupler 322, incident optical 323 is respectively radiated on the detection band 27 of the strip 15 of test strip card 1 and quality control band 28 through strip detection band fibre-optical probe and strip quality control band fibre-optical probe to excite the quantum dot reaction signal thing of strip detection band 27 and quality control band 28 to send characteristic frequency reflected fluorescent light, and reflected fluorescent light enters to be detected in photoelectric detector 4 after band fibre-optical probe and quality control band fibre-optical probe, corresponding the output optical fiber 325, outgoing photo-coupler 326 after testing respectively is converted to electric signal.Fibre-optical probe 324 is incident optical 323 using beam sharing structure, its center is closed, and periphery is the output optical fiber 325.

The photoelectric detector 4 has a variety of available objects, and it includes CCD, CMOS, photomultiplier, photodiode, phototriode.

The data processing equipment 6 is microprocessor, single-chip microcomputer or PC with corresponding data processing and control software.

The electronic label read-write module 10 automatic information of read/write electronic tag 20 in a non contact fashion.

The standby multiple communication interfaces 31 stayed of the detection means 2 are USB interface or RS-232 interface, and it is used for tester and connects printer, external memory medium, host computer, keyboard or other instruments equipment according to outside the detection interim grafting of needs.

The output display device 8 is alphanumeric LCD liquid crystal displays, LED, touch display screen, audio unit or host computer etc..

The power supply 7 is external power supply or disconnectable battery.The battery includes lithium battery.

The detection means 2 of the strip detecting system be on the whole portable instrument, in/large-scale instrument or be and with it is corresponding transmitting and receive information function wireless telecommunications product combination including and mobile phone, tablet personal computer, personal digital assistant, mobile terminal device or set of computers in the combination for selecting product.

Strip 15 in the test strip card 1 is disposable strip；It is the use product matched with batch strip for the electronic tag 20 installed in the cartridge 16 and cartridge 16 of the test strip card 1 that inserts strip 15；The detection means 2 is to continue universal product.

During sample detection, strip 15 is inserted in test strip card 1, the test strip card 1 for inserting strip is inserted into the test strip card jack 33 of detection means 2 again, the interference external part of strip 15（That is the end of sample suction pad 21）It is impregnated in fluid sample.Checking matter fluid sample is acted on slowly to the rear end bleeding of strip 15 under the pulling positioned at the adsorptive pads 24 of the strip other end using the capillary siphoning of miillpore filter.When there is target checking matter molecule in fluid sample（Such as antigen or antibody）When, they just be coated in the label pad 22 in the preceding stage casing of strip it is corresponding by quantum dot-labeled good immune molecule combine and together with to strip rear end bleeding to the detection band 27 of strip analyzing film 23, another particular target checking matter correlated response molecule with being coated on detection band 27（Such as another specific antibody or antigen of respective objects checking matter molecule）Combine to form detection band quantum dot reaction signal thing and dam and be enriched in detection band 27.Remaining a part of quantum dot immune mark molecule continues to move ahead bleeding to strip quality control band 28, the Quality Control thing with being coated on quality control band 28（Such as secondary antibody）Quality control band quantum dot reaction signal thing is combined to form, and dams and is enriched on quality control band 28.The quantum dot reaction signal thing of strip detection band 27 sends characteristic frequency reflected fluorescent light after being excited by the light that optical system 3 is sent, the quantum dot fluorescence signal that strip detection band 27 reflects is proportionate with testing sample concentration.Checking matter concentration is bigger in sample, and the characteristic frequency fluorescence signal that strip detection band 27 is sent is stronger.Conversely, checking matter concentration is smaller in sample, the characteristic frequency fluorescence signal that strip detection band 27 is sent is weaker, thus can quantify checking matter concentration in sample.The light that optical system 3 is sent excites the quantum dot reaction signal thing of strip detection band 27 and quality control band 28 to send reflected fluorescent light, the reflected fluorescence signal is received by photoelectric detector 4 and is converted into electric signal, electric signal after conversion is transferred to A/D converter 5 and is converted to data signal and is transferred to data processing equipment 6 after amplifying through signal, the characteristic frequency fluorescence signal that 6 pairs of transmission of data processing equipment come carries out automatic identification, and the checking matter standard working curve for being sent in test strip card electronic tag 20 come or figure parameters progress checking matter concentration calculating and technical Analysis are transmitted with reference to electronic label read-write module 10, result is transferred to output display device 8, the voice message testing result information of voice module 18.

Checking matter standard working curve preparation method comprises the following steps：（1）Prepare checking matter standard items series concentration.（2）Detection obtains the corresponding OD of checking matter standard items series concentration_{Detection band}And OD_{Quality control band}, and calculate acquisition OD_{Detection band}/OD_{Quality control band}Ratio or OD_{Detection band}/（OD_{Detection band}+OD_{Quality control band}）Ratio.（3）X-axis is made with standard items series concentration, test result is with OD_{Detection band}/OD_{Quality control band}Ratio makees Y-axis, draws standard working curve；Or X-axis is made with standard items series concentration, test result is with OD_{Detection band}/（OD_{Detection band}+OD_{Quality control band}）Ratio makees Y-axis, draws standard working curve；Or X-axis is made with standard items series concentration, test result is with OD_{Detection band}Value makees Y-axis, draws standard working curve.（4）Standard of compiling working curve software.（5）The strip quality control band OD value obtained when standard items are tested is used as strip quality control band optical density reference monitor value（That is OD_{Quality control band '}）；By standard working curve software and OD_{Quality control band '}It is stored in the lump on electronic tag 20.

The detection application method of the strip detecting system is realized by following step：

（1）Start switches on power；

（3）Strip sample suction end impregnated sample；

（4）Pen-down detection key, the quantum dot strip detecting system starts sample detection.After detection terminates, output display device 8 shows strip testing result information, the voice message testing result information of voice module 18.

Embodiment 2

The present embodiment is that its detection means 2 of quantum dot strip detecting system of the present invention can carry out a kind of preferred technique scheme of remote data management and feedback of the information using mobile phone pattern, testing result.5-18 illustrates technical scheme described in the present embodiment below in conjunction with the accompanying drawings.

Fig. 5-8 is the structured flowchart of quantum dot strip detecting system technical scheme described in the present embodiment.Wherein, Fig. 5 is the system appearance structured flowchart.Fig. 6-8 is the internal structure block diagram of the detection means 2 of the system.

In Fig. 5 combinations Fig. 6-8, the quantum dot immune chromatographs strip detecting system, including test strip card 1, detection means 2 and one Radio Network System 13,14 including remote server 14 or/and other mobile terminal devices 14.Wherein, the test strip card 1 includes cartridge 16 and the quantum dot immune that can be inserted into good time in cartridge 16 chromatography strip 15.The detection means 2 includes optical system 3, photoelectric detector 4, A/D converter 5, data processing equipment 6, electronic label read-write module 10, output display device 8, voice module 18, wireless communication module 12, power supply 7, the button 9 positioned at the surface of detection means 2 and the standby multiple communication interfaces 31 for being available for connecting outside interim grafting stayed in detection means 2.The imaging signal end of optical system 3 is connected with photoelectric detector 4.The signal output part of photoelectric detector 4 is connected after amplifying through signal with the signal input part of A/D converter 5.The signal output part of A/D converter 5 is connected with the signal input part of data processing equipment 6.Data processing equipment 6 is also respectively with wireless communication module 12, output display device 8, voice module 18, electronic label read-write module 10, power supply 7, the button 9 on the surface of detection means 2 and standby staying each communication interface 31 in detection means 2 to be connected.The signal transmitting terminal of wireless communication module 12 communicates with the Radio Network System 13,14 including remote server 14 or/and other mobile terminal devices 14, and its communication modes includes but is not limited to Wifi, bluetooth, short message, wechat, mobile phone etc..Power supply 7 is powered by data processing equipment 6 to optical system 3, photoelectric detector 4, A/D converter 5, output display device 8, voice module 18 and electronic label read-write module 10.Power supply 7 is also powered to wireless communication module 12.There is a test strip card jack 33 front end of detection means 2.Test strip card 1 is the separated type plug structure of detection means 2.Strip 15 is the separated type plug structure of test strip card 1.Test strip card 1 engages slot mark 19 at the test strip card jack 33 corresponding to detection means 2 provided with strip.The sample in strip is detected in the test strip card jack 33 for inserting detection means 2 after the insertion test strip card 1 of strip 15 in the lump together with test strip card 1.

One end of test strip card cartridge 16 forms strip slot mouthful 17 together with the upper box face opening at the cartridge end and inserts strip 15 to facilitate.The side view structure of the strip 15 of cartridge 16 shown in Figure 11,12,15,16,18.Wherein, the strip 15 in cartridge 16 shown in Figure 11-12 includes sequentially overlapping the sample suction pad 21 being fixed on end liner 26, label pad 22, analyzing film 23, adsorptive pads 24.Strip 15 shown in Figure 15 includes sequentially overlapping the sample suction pad 21 being fixed on end liner 26, filter sample pad 11, label pad 22, analyzing film 23, adsorptive pads 24.Strip 15 shown in Figure 16 includes sequentially overlapping the sample suction pad 21 being fixed on end liner 26, label pad 22, analyzing film 23, adsorptive pads 24, strip reaction end indicating label 25.Strip 15 shown in Figure 18 includes sequentially overlapping the sample suction pad 21 being fixed on end liner 26, filter sample pad 11, label pad 22, analyzing film 23, adsorptive pads 24, strip reaction end indicating label 25.The analyzing film 23 of the strip 15 has detection band 27 and quality control band 28, and detection band 27 is one or more（Fig. 6,8-11,13, the detection band 27 of the analyzing film 23 of strip 15 shown in 15-18 are one；The detection band 27 of the analyzing film 23 of strip 15 shown in Fig. 7,12,14 is a plurality of）.The plan structure of test strip card 1 shown in Figure 13,14,17.The upper box face of test strip card cartridge 16 is provided with detection window 29 at corresponding to the position of strip analyzing film 23.Cartridge 16 is provided with strip reaction end observation window 30 at the position corresponding to strip reaction end indicating label 25（Shown in Figure 17）.Any place of cartridge 16 is provided with the electronic tag 20 of the detection information of strip 15 that is stored with.When strip 15 is inserted into test strip card cartridge 16, the detection correspondence of window 29 of cartridge 16 is located at the photoirradiated surface of the optical system 3 of detection means 2.

During sample detection, its one end interference of sample suction pad 21 of the strip 15 being inserted into test strip card cartridge 16 stretches out the openend of test strip card cartridge 16 to impregnate pipette samples.The light that optical system 3 is sent excites the quantum dot reaction signal thing of strip detection band 27 and quality control band 28 to send reflected fluorescent light, the reflected fluorescence signal is received by photoelectric detector 4 and is converted into electric signal, electric signal after conversion is transferred to A/D converter 5 and is converted to data signal and is transferred to data processing equipment 6 after amplifying through signal, the characteristic frequency fluorescence signal that 6 pairs of transmission of data processing equipment come carries out automatic identification, and the checking matter standard working curve for being sent in test strip card electronic tag 20 come or figure parameters progress checking matter concentration calculating and technical Analysis are transmitted with reference to electronic label read-write module 10, result is transferred to output display device 8, the voice message testing result information of voice module 18, testing result and its relevant information are sent to Radio Network System 13 by wireless communication module 12, 14 remote server 14 or/and mobile terminal device 14 carries out data management and fed back with information consultation.

（1）Start switches on power；

（3）Strip sample suction end impregnated sample；

（4）Pen-down detection key, the quantum dot strip detecting system starts sample detection.After detection terminates, output display device 8 shows strip testing result information, the voice message testing result information of voice module 18；

Embodiment 3

Quantum dot immune described in the present embodiment chromatographs strip detecting system such as embodiment 1 or 2, differs only in, wherein together with the data processing equipment 6 is integrated in described electronic label read-write module 10.During sample detection, the data processing equipment 6 for being integrated with electronic label read-write module 10 transmits the characteristic frequency fluorescence signal come to detection band 27 and quality control band 28 and carries out automatic identification and combine the checking matter standard working curve sent from test strip card electronic tag 20 or figure parameters progress checking matter concentration calculating and technical Analysis.As shown in Figure 4 and Figure 8.

Embodiment 4

Quantum dot immune described in the present embodiment chromatographs strip detecting system such as embodiment 1,2 or 3, differ only in, the filter sample pad 11 for sample separating treatment is also overlapped between the sample suction pad 21 and label pad 22 of quantum dot strip 15 in wherein described test strip card cartridge 16, as shown in Figure 15 and Figure 18.The filter sample pad 11 includes hemofiltration film.For example, when the strip detecting system is used to detect blood sample, the serum that the filter sample pad 11 can prevent haemocyte and only allow in blood sample oozes filtering sample pad 11, blood sample detection just thus can be carried out with whole blood, the deficiency that could carry out detecting after serum must be separated with instrument and equipments such as centrifuges by overcoming prior art detection blood sample.

Embodiment 5

Quantum dot immune described in the present embodiment chromatographs strip detecting system such as embodiment 1,2,3 or 4, differs only in：In wherein described test strip card cartridge 16 a strip reaction end indicating label 25 is also overlapped with after the adsorptive pads 24 of strip 15.Test strip card cartridge 16 is provided with strip reaction end observation window 30 at the position corresponding to the strip reaction end indicating label 25, as shown in figs. 16-18.The strip reaction end indicating label 25 includes but is not limited to the accurate pH test paper that color change interval is 5.0-9.0.During sample detection, whether the strip reaction end indicating label 25 is to point out siphon bleeding chromatography of the strip reactant in strip abundant, and whether reaction reaches home.

Embodiment 6

Quantum dot immune described in the present embodiment chromatographs strip detecting system such as embodiment 1,2,3,4 or 5, differs only in, wherein, the electronic tag 20 of the detection information of the strip that is stored with 15 is arranged on any place of strip 15 in cartridge 16（Figure of description is not shown）.

Embodiment 7

The present embodiment combination Figure 19-20 illustrates that quantum dot immune chromatography strip detecting system of the present invention is used to simultaneously and rapidly quantitatively detect blood serum tumor markers alpha-fetoprotein（AFP）, carcinomebryonic antigen（CEA）And prostate specific antigen（PSA）（One inspection is multinomial）.

1st, multinomial strip is examined for AFP, CEA and PSA quantum dot one synchronously detected to make

The quantum dot immune chromatography strip 15 is sequentially provided with the sample suction pad 21 that mutually overlap joint is pasted, glass fibre membrane label pad 22, the analyzing film 23 with detection band 27 and quality control band 28, adsorptive pads 24.Glass fibre membrane label pad 22 is coated with the mixture of quantum dot-labeled AFP monoclonal antibodies, quantum dot-labeled CEA monoclonal antibodies and quantum dot-labeled PSA monoclonal antibodies；Detection band 27 is coated with the mixture of AFP antibody, CEA antibody and PSA antibody；Quality control band 28 is coated with secondary antibody Quality Control thing such as sheep anti mouse IgM antibody or sheep anti-mouse igg antibody or Rabbit anti-IgM antibody or rabbit anti-mouse igg antibody.

（1）It is prepared by strip component

1. sample suction pad 21: From cellulose membrane, 297 × 15mm film blocks are cut into, are put in elongate slots, plus sample suction pad treatment fluid（PH=7.2,0.03mol/L phosphate buffer+5%BSA+0.1%Tween 20）Soak at room temperature 30min.Take out film block, 37 DEG C of drying for standby.

2. label pad 22：From glass fibre membrane, 297 × 10mm film blocks are cut into, are put in elongate slots, plus preprepared quantum dot-labeled thing solution（Contain quantum dot-labeled AFP monoclonal antibodies, quantum dot-labeled CEA monoclonal antibodies, the mixture solution of quantum dot-labeled PSA monoclonal antibodies）Thereon, film block, 37 DEG C of drying for standby are taken out.

3. analyzing film 23：From nitrocellulose filter, 297 × 25mm film blocks are cut into, are put in elongate slots, by lower from upper specking AFP antibody separated by a distance from film block base（0.5-5mg/ml）, CEA antibody（0.5-5mg/ml）With PSA antibody（0.5-5mg/ml）Mixture make detection band 27, specking 0.5-5mg/ml sheep anti mouses IgM antibody or sheep anti-mouse igg antibody or Rabbit anti-IgM antibody or rabbit anti-mouse igg antibody make quality control band 28, afterwards by be film-made block in 37 DEG C of drying for standby.

4. adsorptive pads 24：From the cellulose membrane with strong water sorption, 297 × 30mm film blocks, abundant drying for standby are cut into.

（2）Strip is assembled

Each strip component prepared is sequentially mutually overlapped by sample suction pad 21, label pad 22, analyzing film 23, adsorptive pads 24 and is pasted on plastics end liner 26, and cuts into the quantum dot one of certain specification and examines multinomial strip, kept dry is standby.

2nd, standard working curve is drawn and storage

Figure 19 is AFP, CEA, PSA examination criteria working curve.Its preparation method is as follows：

（1）Standard items series concentration is prepared

With 1:The normal human serum of 10 dilutions is (with pH=7.2,0.03mol/L PB buffer solutions dilute) make dilution, AFP, CEA and PSA standard items are made into each 20 parts of series concentration by 0pg/ml, 100pg/ml, 200pg/m1,300pg/ml, 400pg/ml, 500pg/ml, 600pg/ml, 700pg/ml, 800pg/ml, 900pg/ml, 1000pg/ml, 1100pg/ml, 1200pg/ml, 1300pg/ml, 1400pg/ml, 1500pg/ml, 1600pg/ml, 1700pg/ml, 1800pg/ml, 1900pg/m1 respectively.

（2）Draw tumor markers examination criteria working curve

Every part of tumor markers standard items series concentration is examined multinomial strip with 10 quantum dot-labeled tumor markerses one respectively and detected 10 times under the conditions of identical systems setting, and its detection band OD value is read to obtain respectively（OD_{Detection band}）With quality control band OD value（OD_{Quality control band}）, obtain average value and its OD_{Detection band}/OD_{Quality control band}Ratio.X-axis is made with each series concentration of AFP, CEA, PSA standard items respectively, the OD tried to achieve with each series concentration correspondence of AFP, CEA, PSA standard items_{Detection band}/OD_{Quality control band}Ratio makees Y-axis and draws standard working curve.As a result Figure 19 is seen.

（3）Write tumor markers examination criteria working curve software, the OD obtained when standard working curve software data is tested together with standard items used_{Quality control band}Value（When sample is actually detected, the OD_{Quality control band}Value is used as strip quality control band optical density reference monitor value OD_{Quality control band '}）And strip detection information（Including strip lot number, strip title, strip effect phase, RFID tag password, clinical criteria reference value, strip production firm etc.）It is stored in the lump on RFID tag 20 etc. data.

（4）The RFID tag 20 of be stored with AFP, CEA, PSA standard working curve and strip detection information is attached and is installed in the cartridge 16 of test strip card.

3rd, Serum of Cancer Patients sample tumor marker detection

（1）Sample source：Blood serum sample is provided by certain tumour hospital laboratory.Blood serum sample pH=7.2,0.03mol/L before detection PB buffer solutions make 10 times of dilutions.

（2）Sample detection：

1. start switches on power；

2. prepared quantum dot-labeled AFP, CEA, PSA mono- is examined into multinomial strip 15 to insert in test strip card 1；The test strip card 1 for inserting strip is inserted into the test strip card jack 33 of the detection means 2 of the strip detecting system in the lump again；

3. strip sample suction end impregnated sample；

4. Pen-down detection key, the strip detecting system starts sample detection.After detection terminates, output display device 8 shows strip testing result information, the voice message testing result information of voice module 18；

5. send key is pressed, the remote server 14 or/and mobile terminal device 14 that testing result and information are sent to Radio Network System 13,14 carry out data management and information consultation feedback.

（3）Serum of Cancer Patients sample AFP, CEA, PSA ultimate density are calculated：

Formula is：Blood serum sample tumor markers ultimate density (pg/m1)=strip detecting system measures concentration × serum diluting multiple

For example：Blood serum sample detects that its AFP concentration is that 1753.4526pg/m1, CEA concentration are that 878.3892pg/m1, PSA concentration are 983.4257pg/m1 through the strip detecting system, then：

Blood serum sample AFP ultimate densities (pg/m1)=1753.4526pg/m1 × 10=17534.526pg/m1

Blood serum sample CEA ultimate densities (pg/m1)=878.3892pg/m1 × 10=8783.892pg/m1

Blood serum sample PSA ultimate densities (pg/m1)=983.4257pg/m1 × 10=9834.257pg/m1

Figure 20 is quantum dot-labeled AFP, CEA, PSA fluorescent spectrum curve.Wherein, I is the AFP spectrum peaks in detection band, and II is the CEA spectrum peaks in detection band, and III is the PSA spectrum peaks in detection band, and IV is the Quality Control object light spectral peak in quality control band.

Embodiment 8

The present embodiment combination Figure 21-22 illustrates that the quantum dot immune chromatography strip detecting system is used for Quantitative detection hepatitis B surface antigen HBsAg（Individual event is detected）.

1st, made for the quantum dot immune chromatography strip that HBsAg is detected

The quantum dot immune chromatography strip 15 is sequentially provided with the sample suction pad 21 that mutually overlap joint is pasted, filter sample pad 11, glass fibre membrane label pad 22, the analyzing film 23 with detection band 27 and quality control band 28, adsorptive pads 24, strip reaction end indicating label 25.Glass fibre membrane label pad 22 is coated with quantum dot-labeled HBsAg monoclonal antibodies, and detection band 27 is coated with HBsAg antibody.Quality control band 28 is coated with secondary antibody Quality Control thing such as sheep anti mouse IgM antibody or sheep anti-mouse igg antibody or Rabbit anti-IgM antibody or rabbit anti-mouse igg antibody.

（1）It is prepared by strip component

1. sample suction pad 21: From cellulose membrane, 297 × 15mm film blocks are cut into, are put in elongate slots, plus confining liquid（PH=7.2,0.03mol/L phosphate buffer+5%BSA ,+0.1%Tween 20）Soak at room temperature 30min.Take out film block, 37 DEG C of drying for standby.

2. sample pad 11 is filtered：Hemofiltration film is selected by filter sizes, certain specification film block is cut into, kept dry is standby.

3. label pad 22：From glass fibre membrane, 297 × 10mm film blocks are cut into, are put in elongate slots, plus preprepared quantum dot-HBsAg monoclonal antibody conjugates solution is thereon, takes out film block, 37 DEG C of drying for standby.

4. analyzing film 23：From nitrocellulose filter, 297 × 25mm film blocks are cut into, are put in elongate slots, by lower from upper specking 0.5-5mg/ml separated by a distance from film block base HBsAg monoclonal antibodies make detection band 27, and specking 0.5-5mg/ml sheep anti mouses IgM antibody or sheep anti-mouse igg antibody or Rabbit anti-IgM antibody or rabbit anti-mouse igg antibody make quality control band 28, afterwards by be film-made block in 37 DEG C of drying for standby.

5. adsorptive pads 24：From the cellulose membrane with strong water sorption, 297 × 30mm film blocks, abundant drying for standby are cut into.

6. strip reaction end indicating label 25： From the accurate pH test paper that color change interval is 5.0-9.0, the film block with 297 × 5mm, abundant drying for standby are cut into.

（2）Strip is assembled

By each strip component prepared, by sample suction pad 21, filter sample pad 11, label pad 22, analyzing film 23, adsorptive pads 24, strip reaction end indicating label 25, sequentially mutually overlap joint is pasted on plastics end liner 26, and the quantum dot immune chromatography strip of certain specification is cut into, kept dry is standby.

2nd, standard working curve is drawn and storage

Figure 21 is HBsAg examination criteria working curves.Its preparation method is as follows：

（1）HBsAg standard items series concentration is prepared

With 1:The normal human serum of 10 dilutions is (with pH=7.2,0.03mol/L PB buffer solutions dilute) make dilution, HBsAg standard items are made into 20 parts of series concentration：0pg/ml、100pg/ml、200pg/m1、300pg/ml、400pg/ml、500pg/ml、600pg/ml、700pg/ml、800pg/ml、900pg/ml、1000pg/ml、1100pg/ml、1200pg/ml、1300pg/ml、1400pg/ml、1500pg/ml、1600pg/ml、1700pg/ml、1800pg/ml、1900pg/m1.

（2）Draw HBsAg examination criteria working curves

Above-mentioned every HBsAg standard items series concentration is detected 10 times with 10 quantum dot-labeled HBsAg strips under the conditions of identical systems setting respectively, and its detection band OD value is read to obtain respectively（OD_{Detection band}）With quality control band OD value（OD_{Quality control band}）, calculate average value and its OD_{Detection band}/（OD_{Detection band}+OD_{Quality control band}）Ratio.X-axis is made with HBsAg standard items series concentrations, the OD tried to achieve with every HBsAg standard items series concentration correspondence_{Detection band}/（OD_{Detection band}+OD_{Quality control band}）Ratio makees Y-axis and draws standard working curve, as a result sees Figure 21.

（3）Write HBsAg examination criteria working curve softwares, the OD obtained when standard working curve software data is tested together with standard items used_{Quality control band}Value（When sample is actually detected, the OD_{Quality control band}Value is used as strip quality control band optical density reference monitor value OD_{Quality control band '}）And strip detection information（Including strip lot number, strip title, strip effect phase, IC card chip password, clinical criteria reference value, strip production firm etc.）It is stored in the lump in IC card chip 20 etc. data.

（4）The IC card chip 20 of be stored with HBsAg examination criterias working curve and strip detection information is attached to the cartridge for being installed on test strip card（16）On.

3rd, Serum of Patients with Hepatitis B sample HBsAg is detected

（1）Sample source：Serum of Patients with Hepatitis B sample is provided by certain healthcare hospital for women ＆ children, blood serum sample pH=7.2,0.03mol/L before detection PB buffer solutions make 10 times of dilutions.

（2）Sample detection：

1. start switches on power；

2. the prepared quantum dot immune chromatography strip 15 for being used for HBsAg detections is inserted in test strip card 1；The test strip card 1 for inserting strip is inserted into the test strip card jack 33 of the detection means 2 of the strip detecting system in the lump again；

3. strip sample suction end impregnated sample；

（3）Serum of Patients with Hepatitis B sample HBsAg ultimate densities are calculated：

HBsAg concentration × serum diluting multiple that HBsAg ultimate densities (pg/m1)=strip detecting system is measured in blood serum sample

For example：Blood serum sample detects that its HBsAg concentration is 205.9563 pg/m1 through the strip detecting system.Thus：

Blood serum sample HBsAg ultimate densities (pg/m1)=205.9563pg/m1 × 10=2059.563pg/m1

Figure 22 is quantum dot-labeled HBsAg fluorescent spectrum curves.Wherein, I is the HBsAg spectrum peaks in detection band, and II is the Quality Control object light spectral peak in quality control band.

Above example and its accompanying drawing are merely to further illustrate the present invention, and those skilled in the art should not be limited the scope of the invention with this.In particular, quantum dot immune chromatography strip detecting system of the present invention can also have other improvement, detected as strip can be also arranged in strip cartridge by the left end of test strip card 1 using sealing mode, test strip card 1 in such cases typically sets well 32 to be loaded in the corresponding upper surface of cartridge 16 of strip sample pad 21（Such as Figure 23）；The strip 15 being inserted into cartridge also can be replaced biochip（Including antigen-antibody chip, protein chip, DNA chip, micro-fluidic chip）Etc..Therefore, every other technical schemes formed to strip detecting system of the present invention using any equivalent substitution or equivalent transformation, are all fallen within the protection domain of the claims in the present invention.

Claims

A kind of quantum dot immune chromatographs strip detecting system, including quantum dot immune chromatography strip（15）And detection means（2）, quantum dot immune chromatography strip（15）Including sequentially mutually overlap joint is fixed on end liner（26）On sample suction pad（21）, label pad（22）, analyzing film（23）, adsorptive pads（24）, analyzing film（23）With detection band（27）And quality control band（28）, label pad（22）It is coated with one or more by the quantum dot-labeled correlation molecule that target is detected analyte detection that is used for, detection band（27）It is coated with one or more another molecules related to the tested analyte detection of target, quality control band（28）It is coated with the Quality Control thing including secondary antibody；Detection means（2）Including optical system（3）, photoelectric detector（4）, A/D converter（5）, data processing equipment（6）, output display device（8）, optical system（3）Imaging signal end and photoelectric detector（4）It is connected, photoelectric detector（4）Signal output part amplify through signal after with A/D converter（5）Signal input part be connected, A/D converter（5）Signal output part and data processing equipment（6）Signal input part be connected, data processing equipment（6）With output display device（8）It is connected, it is characterised in that：

The quantum dot immune chromatography strip of the system（15）With the strip cartridge being included in outside it（16）Constitute test strip card（1）, strip（15）For test strip card（1）Separated type plug structure, test strip card（1）For detection means（2）Separated type plug structure；

The strip（15）Detection band（27）For one or more；

The strip cartridge（16）Any place be provided with one and be stored with strip（15）The electronic tag of detection information（20）, cartridge（16）One end together with the cartridge end upper box face opening formation strip slot mouthful（17）, cartridge（16）Upper box face corresponding to strip analyzing film（23）Position at be provided with detection window（29）；

The detection means（2）Also include electronic label read-write module（10）, voice module（18）, power supply（7）With positioned at detection means（2）The button on surface（9）And standby stay in detection means（2）On multiple be available for the interim outer communication interfaces connected（31）, its respectively with data processing equipment（6）It is connected, power supply（7）Pass through data processing equipment（6）To optical system（3）, photoelectric detector（4）, A/D converter（5）, output display device（8）, voice module（18）And electronic label read-write module（10）Power supply, detection means（2）On have a test strip card jack（33）；

The test strip card（1）Corresponding to detection means（2）Test strip card jack（33）Place engages slot mark provided with strip（19）；

Strip（15）Insert test strip card（1）Afterwards together with test strip card（1）Detection means is inserted in the lump（2）Test strip card jack（33）In the sample in strip is detected, be inserted into cartridge（16）Interior strip（15）Its sample suction pad（21）Strip cartridge is stretched out in one end interference（16）Openend to impregnate pipette samples, be inserted into strip cartridge（16）Interior strip（15）Its analyzing film（23）Corresponding detection window（27）Correspondence is located at detection means（2）Optical system（3）Photoirradiated surface, optical system（3）The light sent excites the detection band of strip（27）And quality control band（28）Quantum dot reaction signal thing send reflected fluorescent light, reflected fluorescence signal is by photoelectric detector（4）Receive and be converted into electric signal, the electric signal after conversion is transferred to A/D converter after amplifying through signal（5）Be converted to data signal and be transferred to data processing equipment（6）, data processing equipment（6）The characteristic frequency fluorescence signal come to transmitting carries out automatic identification and combines electronic label read-write module（10）What transmission came is sent in test strip card electronic tag（20）Checking matter standard working curve or figure parameters carry out the calculating of checking matter concentration and technical Analysis, result is transferred to output display device（8）, voice module（18）Voice message testing result information.
Strip detecting system according to claim 1, it is characterised in that：The test strip card（1）Electronic tag（20）IC card chip including RFID tag, non contact angle measurement.
Strip detecting system according to claim 1, it is characterised in that：The detection means（2）Optical system（3）Light source be LED or laser diode.
Strip detecting system according to claim 1, it is characterised in that：The detection means（2）Photoelectric detector（4）For CCD, CMOS, photomultiplier, photodiode or phototriode.
Strip detecting system according to claim 1, it is characterised in that：The detection means（2）Data processing equipment（6）For microprocessor, single-chip microcomputer or PC with corresponding data processing and control software.
System according to claim 1, it is characterised in that：The detection means（2）Output display device（8）For alphanumeric LCD liquid crystal displays, LED, audio unit, touch display screen or host computer.
Strip detecting system according to claim 1, it is characterised in that：The detection means（2）The upper standby multiple communication interfaces for being available for connecting outside interim stayed（31）Including USB interface or RS-232 interface.
Strip detecting system according to claim 1, it is characterised in that：The electronic tag（20）The strip detection of storage is included with batch quantum dot immunity-chromatography test strip with information（15）The standard working curve or figure parameters, strip quality control band optical density reference monitor value of quantitative sample checking matter concentration（OD_{Quality control band '}）, strip title, strip lot number, strip effect the phase, electronic tag password, clinical indices reference value, strip production firm information, and can read in measurand identity information, tester's information, sample ID, sample number into spectrum, detection the date, testing result information.
According to the strip detecting system described in claim 8, it is characterised in that：The electronic tag（20）The checking matter standard working curve of storage has diversified forms available, and it includes one of following curves：

Checking matter standard items series concentration and OD_{Detection band}/OD_{Quality control band}Between corresponding relation curve；Or,

Checking matter standard items series concentration and OD_{Detection band}/（OD_{Detection band} +OD_{Quality control band}）Between corresponding relation curve；Or,

Checking matter standard items series concentration and OD_{Detection band}Between corresponding relation curve；

The OD_{Detection band}It is defined as the detection band OD value that checking matter standard items series concentration is measured, OD_{Quality control band} It is defined as the quality control band OD value that checking matter standard items series concentration is measured.
Strip detecting system according to claim 1, it is characterised in that：It is inserted into the test strip card（1）Interior strip（15）For disposable strip；For inserting strip（15）Test strip card（1）Cartridge（16）And cartridge（16）The electronic tag of upper installation（20）The use product matched for same batch strip；The detection means（2）For lasting universal product.
Strip detecting system according to claim 1, it is characterised in that：Wherein described detection means（2）Be on the whole portable instrument, in/large-scale instrument or be and with it is corresponding transmitting and receive information function wireless telecommunications product combination including and mobile phone, tablet personal computer, personal digital assistant, mobile terminal device or set of computers in the combination for selecting product.
Strip detecting system according to claim 1, it is characterised in that：Wherein, strip label pad（22）The described quantum dot for being used for the tested analyte detection related molecular marker of target includes ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag₂S、CdS/PbS、CdS/Cd(0H)₂、CdS/HgS、CdS/HgS/CdS、ZnS/CdS、ZnS/CdS/ZnS、ZnS/HgS/ZnS/CdS、CdSe/CdS、CdSe/ZnS、CdSe/ZnSe、CdSe/CuSe、CdSe/HgTe、CdSe/HgSe、CdSe/HgSe/CdSe、CdTe/HgS、CdTe/HgTe、InAs/InP、InAs/CdSe、InAs/ZnSe、MgS、MgSe、MgTe、CaS、CaSe、CaTe、SrS、SrSe、SeTe、BaS、BaSe、BaTe、CdS:Mn、ZnS:Mn、CdS:Cu、ZnS:Cu、CdS:Tb、ZnS:The combination of any one or any several nano-particles in Tb, and by any one above-mentioned quantum dot is core, silica is shell core-shell type nano-complex particle.
According to the system described in claim 1, it is characterised in that：Wherein described optical system（3）Structure composition have kinds of schemes available, it includes：

a) The optical system（3）Including an illuminator and imaging system, illuminator includes an excitation source（311）, in the light source（311）Output light path on be followed successively by fibre bundle（312）, collimated illumination lens（313）, dichronic mirror（314）, front lens group（315）, until test strip card（1）；Imaging system includes the front lens group of common optical axis（315）, dichronic mirror（314）, optical filter（316）, rear microscope group（317）, dichronic mirror（314）Reflecting surface and optical axis included angle are into 45o angles, front lens group（315）With rear microscope group（317）Isolating construction is used, illuminator light path is in dichronic mirror（314）Forward part and imaging system optical axis it is perpendicular, illuminator light path is in dichronic mirror（314）Part afterwards and imaging system common optical axis；Photoelectric detector（4）Positioned at rear microscope group（317）In image planes；Fibre bundle（312）By light source（311）The light sent is divided into the at regular intervals and intensity identical laser beam of tool, and the laser beam is by collimated illumination lens（313）Collimate as parallel beam incident to dichronic mirror（314）Surface, through dichronic mirror（314）Pass through front lens group after reflection（315）Synchronously it is irradiated to positioned at front lens group（315）Object plane test strip card（1）Strip（15）Detection band（27）And quality control band（28）On excite bleeding to detection band（27）And quality control band（28）Quantum dot reaction signal thing send characteristic frequency reflected fluorescent light, detection band（27）And quality control band（28）Reflected fluorescent light is through same front lens group（315）, through dichronic mirror（314）, optical filter（316）Go out rear microscope group after filtering off veiling glare（317）Into photoelectric detector（4）In be detected be converted to electric signal；Or,

b) The optical system（3）Including an excitation source（321）, in the excitation source（321）Output light path on be followed successively by incident photo-coupler（322）, incident optical（323）, fibre-optical probe（324）, the output optical fiber（325）, outgoing photo-coupler（326）, until photoelectric detector（4）；Light source（321）The light sent is through incident photo-coupler（322）Beam splitting enters incident optical（323）, incident optical（323）Test strip card is respectively radiated to through strip detection band fibre-optical probe and strip quality control band fibre-optical probe（1）Strip（15）Detection band（27）And quality control band（28）On to excite strip detection band（27）And quality control band（28）Quantum dot reaction signal thing send characteristic frequency reflected fluorescent light, reflected fluorescent light band fibre-optical probe and quality control band fibre-optical probe, corresponding the output optical fiber after testing respectively（325）, outgoing photo-coupler（326）Enter photoelectric detector afterwards（4）In be detected be converted to electric signal；Fibre-optical probe（324）Using beam sharing structure is closed, its center is incident optical（323）, periphery is the output optical fiber（325）.
Strip detecting system according to claim 1, it is characterised in that：The power supply is external power supply or is disconnectable battery.
A kind of strip detecting system as claimed in claim 1, its feature in：Wherein described data processing equipment（6）And electronic label read-write module（10）Integrate the data processing equipment described in constituting（6）, the described data processing equipment（6）To detection band（27）And quality control band（28）The characteristic frequency fluorescence signal that transmission comes carries out automatic identification and combined from test strip card electronic tag（20）The checking matter standard working curve or figure parameters sent carries out the calculating of checking matter concentration and technical Analysis, and result is transferred into output display device（8）, voice module（18）Voice message testing result information.
Strip detecting system according to claim 15, it is characterised in that：The test strip card（1）Electronic tag（20）IC card chip including RFID tag, non contact angle measurement.
Strip detecting system according to claim 15, it is characterised in that：The detection means（2）Optical system（3）Light source be LED or laser diode.
Strip detecting system according to claim 15, it is characterised in that：The detection means（2）Photoelectric detector（4）For CCD, CMOS, photomultiplier, photodiode or phototriode.
Strip detecting system according to claim 15, it is characterised in that：The detection means（2）Data processing equipment（6）For microprocessor, single-chip microcomputer or PC with corresponding data processing and control software.
Strip detecting system according to claim 15, it is characterised in that：The detection means（2）Output display device（8）For alphanumeric LCD liquid crystal displays, LED, audio unit, touch display screen or host computer.
According to the strip detecting system described in claim 15, it is characterised in that：The detection means（2）The upper standby multiple communication interfaces for being available for connecting outside interim stayed（31）Including USB interface or RS-232 interface.
Strip detecting system according to claim 15, it is characterised in that：The electronic tag（20）The strip detection of storage is included with batch quantum dot immunity-chromatography test strip with information（15）The standard working curve or figure parameters, strip quality control band optical density reference monitor value of quantitative sample checking matter concentration（OD_{Quality control band '}）, strip title, strip lot number, strip effect the phase, electronic tag password, clinical indices reference value, strip production firm information, and can read in measurand identity information, tester's information, sample ID, sample number into spectrum, detection the date, testing result information.
According to the strip detecting system described in claim 22, it is characterised in that：The electronic tag（20）The checking matter standard working curve of storage has diversified forms available, and it includes one of following curves：

Checking matter standard items series concentration and OD_{Detection band}/OD_{Quality control band}Between corresponding relation curve；Or,

Checking matter standard items series concentration and OD_{Detection band}/（OD_{Detection band}+OD_{Quality control band}）Between corresponding relation curve；Or,

Checking matter standard items series concentration and OD_{Detection band}Between corresponding relation curve；

The OD_{Detection band}It is defined as the detection band OD value that checking matter standard items series concentration is measured, OD_{Quality control band}It is defined as the quality control band OD value that checking matter standard items series concentration is measured.
According to the strip detecting system described in claim 15, it is characterised in that：It is inserted into the test strip card（1）Interior strip（15）For disposable strip；For inserting strip（15）Test strip card（1）Cartridge（16）And cartridge（16）The electronic tag of upper installation（20）The use product matched for same batch strip；The detection means（2）For lasting universal product.
Strip detecting system according to claim 15, it is characterised in that：Wherein described detection means（2）Be on the whole portable instrument, in/large-scale instrument or be and with it is corresponding transmitting and receive information function wireless telecommunications product combination including and mobile phone, tablet personal computer, personal digital assistant, mobile terminal device or set of computers in the combination for selecting product.
Strip detecting system according to claim 15, it is characterised in that：Wherein, strip label pad（22）The described quantum dot for being used for the tested analyte detection related molecular marker of target includes ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag₂S、CdS/PbS、CdS/Cd(0H)₂、CdS/HgS、CdS/HgS/CdS、ZnS/CdS、ZnS/CdS/ZnS、ZnS/HgS/ZnS/CdS、CdSe/CdS、CdSe/ZnS、CdSe/ZnSe、CdSe/CuSe、CdSe/HgTe、CdSe/HgSe、CdSe/HgSe/CdSe、CdTe/HgS、CdTe/HgTe、InAs/InP、InAs/CdSe、InAs/ZnSe、MgS、MgSe、MgTe、CaS、CaSe、CaTe、SrS、SrSe、SeTe、BaS、BaSe、BaTe、CdS:Mn、ZnS:Mn、CdS:Cu、ZnS:Cu、CdS:Tb、ZnS:The combination of any one or any several nano-particles in Tb, and by any one above-mentioned quantum dot is core, silica is shell core-shell type nano-complex particle.
According to the strip detecting system described in claim 15, it is characterised in that：Wherein described optical system（3）Structure composition have kinds of schemes available, it includes：

a) The optical system（3）Including an illuminator and imaging system, illuminator includes an excitation source（311）, in the light source（311）Output light path on be followed successively by fibre bundle（312）, collimated illumination lens（313）, dichronic mirror（314）, front lens group（315）, until test strip card（1）；Imaging system includes the front lens group of common optical axis（315）, dichronic mirror（314）, optical filter（316）, rear microscope group（317）, dichronic mirror（314）Reflecting surface and optical axis included angle are into 45o angles, front lens group（315）With rear microscope group（317）Isolating construction is used, illuminator light path is in dichronic mirror（314）Forward part and imaging system optical axis it is perpendicular, illuminator light path is in dichronic mirror（314）Part afterwards and imaging system common optical axis；Photoelectric detector（4）Positioned at rear microscope group（317）In image planes；Fibre bundle（312）By light source（311）The light sent is divided into the at regular intervals and intensity identical laser beam of tool, and the laser beam is by collimated illumination lens（313）Collimate as parallel beam incident to dichronic mirror（314）Surface, through dichronic mirror（314）Pass through front lens group after reflection（315）Synchronously it is irradiated to positioned at front lens group（315）Object plane test strip card（1）Strip（15）Detection band（27）And quality control band（28）On excite bleeding to detection band（27）And quality control band（28）Quantum dot reaction signal thing send characteristic frequency reflected fluorescent light, detection band（27）And quality control band（28）Reflected fluorescent light is through same front lens group（315）, through dichronic mirror（314）, optical filter（316）Go out rear microscope group after filtering off veiling glare（317）Into photoelectric detector（4）In be detected be converted to electric signal；Or,

b) The optical system（3）Including an excitation source（321）, in the excitation source（321）Output light path on be followed successively by incident photo-coupler（322）, incident optical（323）, fibre-optical probe（324）, the output optical fiber（325）, outgoing photo-coupler（326）, until photoelectric detector（4）；Light source（321）The light sent is through incident photo-coupler（322）Beam splitting enters incident optical（323）, incident optical（323）Test strip card is respectively radiated to through strip detection band fibre-optical probe and strip quality control band fibre-optical probe（1）Strip（15）Detection band（27）And quality control band（28）On to excite strip detection band（27）And quality control band（28）Quantum dot reaction signal thing send characteristic frequency reflected fluorescent light, reflected fluorescent light band fibre-optical probe and quality control band fibre-optical probe, corresponding the output optical fiber after testing respectively（325）, outgoing photo-coupler（326）Enter photoelectric detector afterwards（4）In be detected be converted to electric signal；Fibre-optical probe（324）Using beam sharing structure is closed, its center is incident optical（323）, periphery is the output optical fiber（325）.
Strip detecting system according to claim 15, it is characterised in that：The power supply is external power supply or is disconnectable battery.
A kind of strip detecting system as described in claim 1 or 15, its feature in：The system also includes the data processing equipment of one and the system（6）Connected wireless communication module（12）With one and the wireless communication module（12）Signal transmitting terminal communicates including remote server（14）Or/and mobile terminal device（14）Radio Network System inside（13,14）, power supply（7）Also to wireless communication module（12）Power supply, data processing equipment（6）To detection band（27）And quality control band（28）The characteristic frequency fluorescence signal that transmission comes carries out automatic identification and combined from test strip card electronic tag（20）The checking matter standard working curve or figure parameters sent carries out the calculating of checking matter concentration and technical Analysis, and result is transferred into output display device（8）, voice module（18）Voice message testing result information, testing result and its relevant information pass through wireless communication module（12）It is sent to Radio Network System（13,14）Remote server（14）Or/and mobile terminal device（14）Data management is carried out to feed back with information consultation.
Strip detecting system according to claim 29, it is characterised in that：The test strip card（1）Electronic tag（20）IC card chip including RFID tag, non contact angle measurement.
Strip detecting system according to claim 29, it is characterised in that：The detection means（2）Optical system（3）Light source be LED or laser diode.
Strip detecting system according to claim 29, it is characterised in that：The detection means（2）Photoelectric detector（4）For CCD, CMOS, photomultiplier, photodiode or phototriode.
Strip detecting system according to claim 29, it is characterised in that：The detection means（2）Data processing equipment（6）For microprocessor, single-chip microcomputer or PC with corresponding data processing and control software.
Strip detecting system according to claim 29, it is characterised in that：The detection means（2）Output display device（8）For alphanumeric LCD liquid crystal displays, LED, audio unit, touch display screen or host computer.
Strip detecting system according to claim 29, it is characterised in that：The detection means（2）The upper standby multiple communication interfaces for being available for connecting outside interim stayed（31）Including USB interface or RS-232 interface.
Strip detecting system according to claim 29, it is characterised in that：The electronic tag（20）The strip detection of storage is included with batch quantum dot immunity-chromatography test strip with information（15）The standard working curve or figure parameters, strip quality control band optical density reference monitor value of quantitative sample checking matter concentration（OD_{Quality control band '}）, strip title, strip lot number, strip effect the phase, electronic tag password, clinical indices reference value, strip production firm information, and can read in measurand identity information, tester's information, sample ID, sample number into spectrum, detection the date, testing result information.
According to the strip detecting system described in claim 36, it is characterised in that：The electronic tag（20）The checking matter standard working curve of storage has diversified forms available, and it includes one of following curves：

Checking matter standard items series concentration and OD_{Detection band}/OD_{Quality control band}Between corresponding relation curve；Or,

Checking matter standard items series concentration and OD_{Detection band}/（OD_{Detection band}+OD_{Quality control band}）Between corresponding relation curve；Or,

Checking matter standard items series concentration and OD_{Detection band}Between corresponding relation curve；

The OD_{Detection band}It is defined as the detection band OD value that checking matter standard items series concentration is measured, OD_{Quality control band}It is defined as the quality control band OD value that checking matter standard items series concentration is measured.
Strip detecting system according to claim 29, it is characterised in that：It is inserted into the test strip card（1）Interior strip（15）For disposable strip；For inserting strip（15）Test strip card（1）Cartridge（16）And cartridge（16）The electronic tag of upper installation（20）The use product matched for same batch strip；The detection means（2）For lasting universal product.
Strip detecting system according to claim 29, it is characterised in that：Wherein described detection means（2）Be on the whole portable instrument, in/large-scale instrument or be and with it is corresponding transmitting and receive information function wireless telecommunications product combination including and mobile phone, tablet personal computer, personal digital assistant, mobile terminal device or set of computers in the combination for selecting product.
Strip detecting system according to claim 29, it is characterised in that：Wherein, strip label pad（22）The described quantum dot for being used for the tested analyte detection related molecular marker of target includes ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag₂S、CdS/PbS、CdS/Cd(0H)₂、CdS/HgS、CdS/HgS/CdS、ZnS/CdS、ZnS/CdS/ZnS、ZnS/HgS/ZnS/CdS、CdSe/CdS、CdSe/ZnS、CdSe/ZnSe、CdSe/CuSe、CdSe/HgTe、CdSe/HgSe、CdSe/HgSe/CdSe、CdTe/HgS、CdTe/HgTe、InAs/InP、InAs/CdSe、InAs/ZnSe、MgS、MgSe、MgTe、CaS、CaSe、CaTe、SrS、SrSe、SeTe、BaS、BaSe、BaTe、CdS:Mn、ZnS:Mn、CdS:Cu、ZnS:Cu、CdS:Tb、ZnS:The combination of any one or any several nano-particles in Tb, and by any one above-mentioned quantum dot is core, silica is shell core-shell type nano-complex particle.
According to the strip detecting system described in claim 29, it is characterised in that：Wherein described optical system（3）Structure composition have kinds of schemes available, it includes：

a) The optical system（3）Including an illuminator and imaging system, illuminator includes an excitation source（311）, in the light source（311）Output light path on be followed successively by fibre bundle（312）, collimated illumination lens（313）, dichronic mirror（314）, front lens group（315）, until test strip card（1）；Imaging system includes the front lens group of common optical axis（315）, dichronic mirror（314）, optical filter（316）, rear microscope group（317）, dichronic mirror（314）Reflecting surface and optical axis included angle are into 45o angles, front lens group（315）With rear microscope group（317）Isolating construction is used, illuminator light path is in dichronic mirror（314）Forward part and imaging system optical axis it is perpendicular, illuminator light path is in dichronic mirror（314）Part afterwards and imaging system common optical axis；Photoelectric detector（4）Positioned at rear microscope group（317）In image planes；Fibre bundle（312）By light source（311）The light sent is divided into the at regular intervals and intensity identical laser beam of tool, and the laser beam is by collimated illumination lens（313）Collimate as parallel beam incident to dichronic mirror（314）Surface, through dichronic mirror（314）Pass through front lens group after reflection（315）Synchronously it is irradiated to positioned at front lens group（315）Object plane test strip card（1）Strip（15）Detection band（27）And quality control band（28）On excite bleeding to detection band（27）And quality control band（28）Quantum dot reaction signal thing send characteristic frequency reflected fluorescent light, detection band（27）And quality control band（28）Reflected fluorescent light is through same front lens group（315）, through dichronic mirror（314）, optical filter（316）Go out rear microscope group after filtering off veiling glare（317）Into photoelectric detector（4）In be detected be converted to electric signal；Or,

b) The optical system（3）Including an excitation source（321）, in the excitation source（321）Output light path on be followed successively by incident photo-coupler（322）, incident optical（323）, fibre-optical probe（324）, the output optical fiber（325）, outgoing photo-coupler（326）, until photoelectric detector（4）；Light source（321）The light sent is through incident photo-coupler（322）Beam splitting enters incident optical（323）, incident optical（323）Test strip card is respectively radiated to through strip detection band fibre-optical probe and strip quality control band fibre-optical probe（1）Strip（15）Detection band（27）And quality control band（28）On to excite strip detection band（27）And quality control band（28）Quantum dot reaction signal thing send characteristic frequency reflected fluorescent light, reflected fluorescent light band fibre-optical probe and quality control band fibre-optical probe, corresponding the output optical fiber after testing respectively（325）, outgoing photo-coupler（326）Enter photoelectric detector afterwards（4）In be detected be converted to electric signal；Fibre-optical probe（324）Using beam sharing structure is closed, its center is incident optical（323）, periphery is the output optical fiber（325）.
Strip detecting system according to claim 29, it is characterised in that：The power supply is external power supply or is disconnectable battery.
Strip detecting system according to claim 29, it is characterised in that：The wireless communication module（12）With Radio Network System（13,14）Between communication include Wifi, bluetooth, short message, wechat or mobile phone.
A kind of strip detecting system as described in claim 1,15 or 29, its feature in：Wherein, the quantum dot immune chromatography strip（15）Sample suction pad（21）With label pad（22）Between be also overlapped with filter sample pad for sample separating treatment（11）.
Strip detecting system according to claim 44, it is characterised in that：The quantum dot immune chromatographs strip（15）Filter sample pad（11）Including hemofiltration film.
Strip detecting system according to claim 44 or 45, it is characterised in that：The test strip card（1）Electronic tag（20）IC card chip including RFID tag, non contact angle measurement.
Strip detecting system according to claim 44 or 45, it is characterised in that：The detection means（2）Optical system（3）Light source be LED or laser diode.
Strip detecting system according to claim 44 or 45, it is characterised in that：The detection means（2）Photoelectric detector（4）For CCD, CMOS, photomultiplier, photodiode or phototriode.
Strip detecting system according to claim 44 or 45, it is characterised in that：The detection means（2）Data processing equipment（6）For microprocessor, single-chip microcomputer or PC with corresponding data processing and control software.
Strip detecting system according to claim 44 or 45, it is characterised in that：The detection means（2）Output display device（8）For alphanumeric LCD liquid crystal displays, LED, audio unit, touch display screen or host computer.
Strip detecting system according to claim 44 or 45, it is characterised in that：The detection means（2）The upper standby multiple communication interfaces for being available for connecting outside interim stayed（31）Including USB interface or RS-232 interface.
Strip detecting system according to claim 44 or 45, it is characterised in that：The electronic tag（20）The strip detection of storage is included with batch quantum dot immunity-chromatography test strip with information（15）The standard working curve or figure parameters, strip quality control band optical density reference monitor value of quantitative sample checking matter concentration（OD_{Quality control band '}）, strip title, strip lot number, strip effect the phase, electronic tag password, clinical indices reference value, strip production firm information, and can read in measurand identity information, tester's information, sample ID, sample number into spectrum, detection the date, testing result information.
According to the strip detecting system described in claim 52, it is characterised in that：The electronic tag（20）The checking matter standard working curve of storage has diversified forms available, and it includes one of following curves：

Checking matter standard items series concentration and OD_{Detection band}/OD_{Quality control band}Between corresponding relation curve；Or,

Checking matter standard items series concentration and OD_{Detection band}/（OD_{Detection band}+OD_{Quality control band}）Between corresponding relation curve；Or,

Checking matter standard items series concentration and OD_{Detection band}Between corresponding relation curve；

The OD_{Detection band}It is defined as the detection band OD value that checking matter standard items series concentration is measured, OD_{Quality control band}It is defined as the quality control band OD value that checking matter standard items series concentration is measured.
Strip detecting system according to claim 44 or 45, it is characterised in that：It is inserted into the test strip card（1）Interior strip（15）For disposable strip；For inserting strip（15）Test strip card（1）Cartridge（16）And cartridge（16）The electronic tag of upper installation（20）The use product matched for same batch strip；The detection means（2）For lasting universal product.
Strip detecting system according to claim 44 or 45, it is characterised in that：Wherein described detection means（2）Be on the whole portable instrument, in/large-scale instrument or be and with it is corresponding transmitting and receive information function wireless telecommunications product combination including and mobile phone, tablet personal computer, personal digital assistant, mobile terminal device or set of computers in the combination for selecting product.
Strip detecting system according to claim 44 or 45, it is characterised in that：Wherein, strip label pad（22）The described quantum dot for being used for the tested analyte detection related molecular marker of target includes ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag₂S、CdS/PbS、CdS/Cd(0H)₂、CdS/HgS、CdS/HgS/CdS、ZnS/CdS、ZnS/CdS/ZnS、ZnS/HgS/ZnS/CdS、CdSe/CdS、CdSe/ZnS、CdSe/ZnSe、CdSe/CuSe、CdSe/HgTe、CdSe/HgSe、CdSe/HgSe/CdSe、CdTe/HgS、CdTe/HgTe、InAs/InP、InAs/CdSe、InAs/ZnSe、MgS、MgSe、MgTe、CaS、CaSe、CaTe、SrS、SrSe、SeTe、BaS、BaSe、BaTe、CdS:Mn、ZnS:Mn、CdS:Cu、ZnS:Cu、CdS:Tb、ZnS:The combination of any one or any several nano-particles in Tb, and by any one above-mentioned quantum dot is core, silica is shell core-shell type nano-complex particle.
According to the strip detecting system described in claim 44 or 45, it is characterised in that：Wherein described optical system（3）Structure composition have kinds of schemes available, it includes：

a) The optical system（3）Including an illuminator and imaging system, illuminator includes an excitation source（311）, in the light source（311）Output light path on be followed successively by fibre bundle（312）, collimated illumination lens（313）, dichronic mirror（314）, front lens group（315）, until test strip card（1）；Imaging system includes the front lens group of common optical axis（315）, dichronic mirror（314）, optical filter（316）, rear microscope group（317）, dichronic mirror（314）Reflecting surface and optical axis included angle are into 45o angles, front lens group（315）With rear microscope group（317）Isolating construction is used, illuminator light path is in dichronic mirror（314）Forward part and imaging system optical axis it is perpendicular, illuminator light path is in dichronic mirror（314）Part afterwards and imaging system common optical axis；Photoelectric detector（4）Positioned at rear microscope group（317）In image planes；Fibre bundle（312）By light source（311）The light sent is divided into the at regular intervals and intensity identical laser beam of tool, and the laser beam is by collimated illumination lens（313）Collimate as parallel beam incident to dichronic mirror（314）Surface, through dichronic mirror（314）Pass through front lens group after reflection（315）Synchronously it is irradiated to positioned at front lens group（315）Object plane test strip card（1）Strip（15）Detection band（27）And quality control band（28）On excite bleeding to detection band（27）And quality control band（28）Quantum dot reaction signal thing send characteristic frequency reflected fluorescent light, detection band（27）And quality control band（28）Reflected fluorescent light is through same front lens group（315）, through dichronic mirror（314）, optical filter（316）Go out rear microscope group after filtering off veiling glare（317）Into photoelectric detector（4）In be detected be converted to electric signal；Or,

b) The optical system（3）Including an excitation source（321）, in the excitation source（321）Output light path on be followed successively by incident photo-coupler（322）, incident optical（323）, fibre-optical probe（324）, the output optical fiber（325）, outgoing photo-coupler（326）, until photoelectric detector（4）；Light source（321）The light sent is through incident photo-coupler（322）Beam splitting enters incident optical（323）, incident optical（323）Test strip card is respectively radiated to through strip detection band fibre-optical probe and strip quality control band fibre-optical probe（1）Strip（15）Detection band（27）And quality control band（28）On to excite strip detection band（27）And quality control band（28）Quantum dot reaction signal thing send characteristic frequency reflected fluorescent light, reflected fluorescent light band fibre-optical probe and quality control band fibre-optical probe, corresponding the output optical fiber after testing respectively（325）, outgoing photo-coupler（326）Enter photoelectric detector afterwards（4）In be detected be converted to electric signal；Fibre-optical probe（324）Using beam sharing structure is closed, its center is incident optical（323）, periphery is the output optical fiber（325）.
Strip detecting system according to claim 44 or 45, it is characterised in that：The power supply is external power supply or is disconnectable battery.
A kind of strip detecting system as described in claim 1,15,29,44 or 45, its feature in：Wherein, the strip（15）Adsorptive pads（24）Tail end be also overlapped with a strip reaction end indicating label（25）, test strip card cartridge（16）Corresponding to strip reaction end indicating label（25）Position at be provided with strip reaction end observation window（30）.
Strip detecting system according to claim 59, it is characterised in that：The strip reaction end indicating label（25）For pH test paper.
Strip detecting system according to claim 59 or 60, it is characterised in that：The test strip card（1）Electronic tag（20）IC card chip including RFID tag, non contact angle measurement.
Strip detecting system according to claim 59 or 60, it is characterised in that：The detection means（2）Optical system（3）Light source be LED or laser diode.
Strip detecting system according to claim 59 or 60, it is characterised in that：The detection means（2）Photoelectric detector（4）For CCD, CMOS, photomultiplier, photodiode or phototriode.
Strip detecting system according to claim 59 or 60, it is characterised in that：The detection means（2）Data processing equipment（6）For microprocessor, single-chip microcomputer or PC with corresponding data processing and control software.
Strip detecting system according to claim 59 or 60, it is characterised in that：The detection means（2）Output display device（8）For alphanumeric LCD liquid crystal displays, LED, audio unit, touch display screen or host computer.
Strip detecting system according to claim 59 or 60, it is characterised in that：The detection means（2）The upper standby multiple communication interfaces for being available for connecting outside interim stayed（31）Including USB interface or RS-232 interface.
Strip detecting system according to claim 59 or 60, it is characterised in that：The electronic tag（20）The strip detection of storage is included with batch quantum dot immunity-chromatography test strip with information（15）The standard working curve or figure parameters, strip quality control band optical density reference monitor value of quantitative sample checking matter concentration（OD_{Quality control band '}）, strip title, strip lot number, strip effect the phase, electronic tag password, clinical indices reference value, strip production firm information, and can read in measurand identity information, tester's information, sample ID, sample number into spectrum, detection the date, testing result information.
According to the strip detecting system described in claim 67, it is characterised in that：The electronic tag（20）The checking matter standard working curve of storage has diversified forms available, and it includes one of following curves：

Checking matter standard items series concentration and OD_{Detection band}/OD_{Quality control band}Between corresponding relation curve；Or,

Checking matter standard items series concentration and OD_{Detection band}/（OD_{Detection band}+OD_{Quality control band}）Between corresponding relation curve；Or,

Checking matter standard items series concentration and OD_{Detection band}Between corresponding relation curve；

The OD_{Detection band}It is defined as the detection band OD value that checking matter standard items series concentration is measured, OD_{Quality control band}It is defined as the quality control band OD value that checking matter standard items series concentration is measured.
Strip detecting system according to claim 59 or 60, it is characterised in that：It is inserted into the test strip card（1）Interior strip（15）For disposable strip；For inserting strip（15）Test strip card（1）Cartridge（16）And cartridge（16）The electronic tag of upper installation（20）The use product matched for same batch strip；The detection means（2）For lasting universal product.
Strip detecting system according to claim 59 or 60, it is characterised in that：Wherein described detection means（2）Be on the whole portable instrument, in/large-scale instrument or be and with it is corresponding transmitting and receive information function wireless telecommunications product combination including and mobile phone, tablet personal computer, personal digital assistant, mobile terminal device or set of computers in the combination for selecting product.
Strip detecting system according to claim 59 or 60, it is characterised in that：Wherein, strip label pad（22）The described quantum dot for being used for the tested analyte detection related molecular marker of target includes ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag₂S、CdS/PbS、CdS/Cd(0H)₂、CdS/HgS、CdS/HgS/CdS、ZnS/CdS、ZnS/CdS/ZnS、ZnS/HgS/ZnS/CdS、CdSe/CdS、CdSe/ZnS、CdSe/ZnSe、CdSe/CuSe、CdSe/HgTe、CdSe/HgSe、CdSe/HgSe/CdSe、CdTe/HgS、CdTe/HgTe、InAs/InP、InAs/CdSe、InAs/ZnSe、MgS、MgSe、MgTe、CaS、CaSe、CaTe、SrS、SrSe、SeTe、BaS、BaSe、BaTe、CdS:Mn、ZnS:Mn、CdS:Cu、ZnS:Cu、CdS:Tb、ZnS:The combination of any one or any several nano-particles in Tb, and by any one above-mentioned quantum dot is core, silica is shell core-shell type nano-complex particle.
According to the strip detecting system described in claim 59 or 60, it is characterised in that：Wherein described optical system（3）Structure composition have kinds of schemes available, it includes：

a) The optical system（3）Including an illuminator and imaging system, illuminator includes an excitation source（311）, in the light source（311）Output light path on be followed successively by fibre bundle（312）, collimated illumination lens（313）, dichronic mirror（314）, front lens group（315）, until test strip card（1）；Imaging system includes the front lens group of common optical axis（315）, dichronic mirror（314）, optical filter（316）, rear microscope group（317）, dichronic mirror（314）Reflecting surface and optical axis included angle are into 45o angles, front lens group（315）With rear microscope group（317）Isolating construction is used, illuminator light path is in dichronic mirror（314）Forward part and imaging system optical axis it is perpendicular, illuminator light path is in dichronic mirror（314）Part afterwards and imaging system common optical axis；Photoelectric detector（4）Positioned at rear microscope group（317）In image planes；Fibre bundle（312）By light source（311）The light sent is divided into the at regular intervals and intensity identical laser beam of tool, and the laser beam is by collimated illumination lens（313）Collimate as parallel beam incident to dichronic mirror（314）Surface, through dichronic mirror（314）Pass through front lens group after reflection（315）Synchronously it is irradiated to positioned at front lens group（315）Object plane test strip card（1）Strip（15）Detection band（27）And quality control band（28）On excite bleeding to detection band（27）And quality control band（28）Quantum dot reaction signal thing send characteristic frequency reflected fluorescent light, detection band（27）And quality control band（28）Reflected fluorescent light is through same front lens group（315）, through dichronic mirror（314）, optical filter（316）Go out rear microscope group after filtering off veiling glare（317）Into photoelectric detector（4）In be detected be converted to electric signal；Or,

b) The optical system（3）Including an excitation source（321）, in the excitation source（321）Output light path on be followed successively by incident photo-coupler（322）, incident optical（323）, fibre-optical probe（324）, the output optical fiber（325）, outgoing photo-coupler（326）, until photoelectric detector（4）；Light source（321）The light sent is through incident photo-coupler（322）Beam splitting enters incident optical（323）, incident optical（323）Test strip card is respectively radiated to through strip detection band fibre-optical probe and strip quality control band fibre-optical probe（1）Strip（15）Detection band（27）And quality control band（28）On to excite strip detection band（27）And quality control band（28）Quantum dot reaction signal thing send characteristic frequency reflected fluorescent light, reflected fluorescent light band fibre-optical probe and quality control band fibre-optical probe, corresponding the output optical fiber after testing respectively（325）, outgoing photo-coupler（326）Enter photoelectric detector afterwards（4）In be detected be converted to electric signal；Fibre-optical probe（324）Using beam sharing structure is closed, its center is incident optical（323）, periphery is the output optical fiber（325）.
Strip detecting system according to claim 59 or 60, it is characterised in that：The power supply is external power supply or is disconnectable battery.
A kind of strip detecting system as described in claim 1,15,29,44,45,59 or 60, its feature in：Wherein, be stored with strip（15）The electronic tag of detection information（20）Installed in strip cartridge（16）Interior strip（15）Any place.
Strip detecting system according to claim 74, it is characterised in that：The test strip card（1）Electronic tag（20）IC card chip including RFID tag, non contact angle measurement.
Strip detecting system according to claim 74, it is characterised in that：The detection means（2）Optical system（3）Light source be LED or laser diode.
Strip detecting system according to claim 74, it is characterised in that：The detection means（2）Photoelectric detector（4）For CCD, CMOS, photomultiplier, photodiode or phototriode.
Strip detecting system according to claim 74, it is characterised in that：The detection means（2）Data processing equipment（6）For microprocessor, single-chip microcomputer or PC with corresponding data processing and control software.
Strip detecting system according to claim 74, it is characterised in that：The detection means（2）Output display device（8）For alphanumeric LCD liquid crystal displays, LED, audio unit, touch display screen or host computer.
Strip detecting system according to claim 74, it is characterised in that：The detection means（2）The upper standby multiple communication interfaces for being available for connecting outside interim stayed（31）Including USB interface or RS-232 interface.
Strip detecting system according to claim 74, it is characterised in that：The electronic tag（20）The strip detection of storage is included with batch quantum dot immunity-chromatography test strip with information（15）The standard working curve or figure parameters, strip quality control band optical density reference monitor value of quantitative sample checking matter concentration（OD_{Quality control band '}）, strip title, strip lot number, strip effect the phase, electronic tag password, clinical indices reference value, strip production firm information, and can read in measurand identity information, tester's information, sample ID, sample number into spectrum, detection the date, testing result information.
According to the strip detecting system described in claim 81, it is characterised in that：The electronic tag（20）The checking matter standard working curve of storage has diversified forms available, and it includes one of following curves：

Checking matter standard items series concentration and OD_{Detection band}/OD_{Quality control band}Between corresponding relation curve；Or,

Checking matter standard items series concentration and OD_{Detection band}/（OD_{Detection band}+OD_{Quality control band}）Between corresponding relation curve；Or,

Checking matter standard items series concentration and OD_{Detection band}Between corresponding relation curve；

The OD_{Detection band}It is defined as the detection band OD value that checking matter standard items series concentration is measured, OD_{Quality control band}It is defined as the quality control band OD value that checking matter standard items series concentration is measured.
Strip detecting system according to claim 74, it is characterised in that：It is inserted into the test strip card（1）Interior strip（15）For disposable strip；For inserting strip（15）Test strip card（1）Cartridge（16）And detection means（2）For lasting universal product.
Strip detecting system according to claim 74, it is characterised in that：Wherein described detection means（2）Be on the whole portable instrument, in/large-scale instrument or be and with it is corresponding transmitting and receive information function wireless telecommunications product combination including and mobile phone, tablet personal computer, personal digital assistant, mobile terminal device or set of computers in the combination for selecting product.
Strip detecting system according to claim 74, it is characterised in that：Wherein, strip label pad（22）The described quantum dot for being used for the tested analyte detection related molecular marker of target includes ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag₂S、CdS/PbS、CdS/Cd(0H)₂、CdS/HgS、CdS/HgS/CdS、ZnS/CdS、ZnS/CdS/ZnS、ZnS/HgS/ZnS/CdS、CdSe/CdS、CdSe/ZnS、CdSe/ZnSe、CdSe/CuSe、CdSe/HgTe、CdSe/HgSe、CdSe/HgSe/CdSe、CdTe/HgS、CdTe/HgTe、InAs/InP、InAs/CdSe、InAs/ZnSe、MgS、MgSe、MgTe、CaS、CaSe、CaTe、SrS、SrSe、SeTe、BaS、BaSe、BaTe、CdS:Mn、ZnS:Mn、CdS:Cu、ZnS:Cu、CdS:Tb、ZnS:The combination of any one or any several nano-particles in Tb, and by any one above-mentioned quantum dot is core, silica is shell core-shell type nano-complex particle.
According to the strip detecting system described in claim 74, it is characterised in that：Wherein described optical system（3）Structure composition have kinds of schemes available, it includes：

a) The optical system（3）Including an illuminator and imaging system, illuminator includes an excitation source（311）, in the light source（311）Output light path on be followed successively by fibre bundle（312）, collimated illumination lens（313）, dichronic mirror（314）, front lens group（315）, until test strip card（1）；Imaging system includes the front lens group of common optical axis（315）, dichronic mirror（314）, optical filter（316）, rear microscope group（317）, dichronic mirror（314）Reflecting surface and optical axis included angle are into 45o angles, front lens group（315）With rear microscope group（317）Isolating construction is used, illuminator light path is in dichronic mirror（314）Forward part and imaging system optical axis it is perpendicular, illuminator light path is in dichronic mirror（314）Part afterwards and imaging system common optical axis；Photoelectric detector（4）Positioned at rear microscope group（317）In image planes；Fibre bundle（312）By light source（311）The light sent is divided into the at regular intervals and intensity identical laser beam of tool, and the laser beam is by collimated illumination lens（313）Collimate as parallel beam incident to dichronic mirror（314）Surface, through dichronic mirror（314）Pass through front lens group after reflection（315）Synchronously it is irradiated to positioned at front lens group（315）Object plane test strip card（1）Strip（15）Detection band（27）And quality control band（28）On excite bleeding to detection band（27）And quality control band（28）Quantum dot reaction signal thing send characteristic frequency reflected fluorescent light, detection band（27）And quality control band（28）Reflected fluorescent light is through same front lens group（315）, through dichronic mirror（314）, optical filter（316）Go out rear microscope group after filtering off veiling glare（317）Into photoelectric detector（4）In be detected be converted to electric signal；Or,

b) The optical system（3）Including an excitation source（321）, in the excitation source（321）Output light path on be followed successively by incident photo-coupler（322）, incident optical（323）, fibre-optical probe（324）, the output optical fiber（325）, outgoing photo-coupler（326）, until photoelectric detector（4）；Light source（321）The light sent is through incident photo-coupler（322）Beam splitting enters incident optical（323）, incident optical（323）Test strip card is respectively radiated to through strip detection band fibre-optical probe and strip quality control band fibre-optical probe（1）Strip（15）Detection band（27）And quality control band（28）On to excite strip detection band（27）And quality control band（28）Quantum dot reaction signal thing send characteristic frequency reflected fluorescent light, reflected fluorescent light band fibre-optical probe and quality control band fibre-optical probe, corresponding the output optical fiber after testing respectively（325）, outgoing photo-coupler（326）Enter photoelectric detector afterwards（4）In be detected be converted to electric signal；Fibre-optical probe（324）Using beam sharing structure is closed, its center is incident optical（323）, periphery is the output optical fiber（325）.
Strip detecting system according to claim 74, it is characterised in that：The power supply is external power supply or is disconnectable battery.
A kind of strip detecting system as described in claim any one of 1-87 is applied in field of biological medicine.