CN207866710U - Time-resolved fluoroimmunoassay chromatographs detection device and fluorescence immunity analyzer - Google Patents
Time-resolved fluoroimmunoassay chromatographs detection device and fluorescence immunity analyzer Download PDFInfo
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- CN207866710U CN207866710U CN201820154446.3U CN201820154446U CN207866710U CN 207866710 U CN207866710 U CN 207866710U CN 201820154446 U CN201820154446 U CN 201820154446U CN 207866710 U CN207866710 U CN 207866710U
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Abstract
The utility model discloses a kind of time-resolved fluoroimmunoassay chromatography detection device and fluorescence immunity analyzers.The time-resolved fluoroimmunoassay chromatographs detection device and fluorescence immunity analyzer compared with traditional reflection-type detection device, by the both sides that excitation light source and fluorescence detector are set to detection zone, so as to which the fluorescence sent out from the immunochromatography detection sensor of detection zone is received and is detected, relative to traditional reflection-type detection method, the fluorescence signal on immunochromatography detection sensor surface can not only be detected, it can also detect the fluorescence signal inside immunochromatography detection sensor, and then the signal strength of detection can be improved, be conducive to improve detection sensitivity.By test, detection device and fluorescence immunity analyzer are chromatographed using above-mentioned time-resolved fluoroimmunoassay to detect the fluorescence signal of immuno-chromatographic test paper strip, the signal-to-noise ratio of the fluorescence signal received compared with traditional bounce technique effectively improves 10%~20%.
Description
Technical field
The utility model is related to immunochromatography detection fields, chromatograph and detect more particularly, to a kind of time-resolved fluoroimmunoassay
Device and fluorescence immunity analyzer.
Background technology
Immunochromatographic method is a kind of quick diagnosis technology risen both at home and abroad in recent years, and principle is by the anti-of specificity
Body is first fixed on a certain zone of nitrocellulose membrane, when sample (such as urine or blood are immersed in nitrocellulose one end of the drying
It is clear etc.) after, due to capillarity, sample will be moved forward along the film, when being moved to the region for being fixed with antibody, sample
In corresponding antigen specifically bound with the antibody, if the region can be made to show with immune colloid gold or Immunoperoxidase Staining
Certain color, to provide the qualitative or quantitative detection to sample, to realize the immunodiagnosis of specificity.With routine diagnosis side
Method is compared, and immunochromatographic method analyze speed is fast, and whole detection process only need 5-30 minutes；It is easy to operate, it does not need other any
Instrument is not necessarily to professional, and condition is provided for real-time on-site detection；It is more to detect sample type, can be used for medical treatment, health, food
The micro solution of the preparations such as blood, saliva, food, water quality, soil in product safety, environment measuring.
Immuno-chromatographic test paper strip is the atopic and chromatographic technique in conjunction with immune marker and corresponding antigen (antibody)
And it is manufactured.Immuno-chromatographic test paper strip is usually made of sample application zone, reaction zone and suction zones three parts.Contain immune mark in sample application zone
Remember composition granule, usually by glass fibre by immune colloid gold granular absorption in the area；Reaction zone then sprays two response lines, one
For detection line, one is nature controlling line.Detection line is detecting the coating of antibody (antigen) substance and immune labeled composition granule herein
The reactivity of antigen (antigen that antibody or antibody carry)；Nature controlling line is then detecting the coating protein on immune labeled composition granule
The activity and degree of matter, after immune marker is discharged by sample application zone, reacted area partly proceeds to adsorption zone, completes chromatography, from
And achieve the purpose that quickly to detect.
The immunofluorescence chromatographic apparatus used at present is all made of the fluorescence signal on bounce technique detection perforated membrane, fluorescence analysis
What instrument captured is the specific antibody of porous film surface fluorescent dye modification, and is difficult to detect the fluorescence inside perforated membrane and believes
Number, cause detection sensitivity to decline.
Utility model content
Based on this, it is necessary to provide a kind of signal strength that can improve detection, and then improve the time of detection sensitivity
Resolved fluorometric immuno-chromatography detection device and fluorescence immunity analyzer.
A kind of time-resolved fluoroimmunoassay chromatographs detection device, including pulse excitation light source, sensor mounting block and glimmering
Photodetector；The sensor mounting block has the sensor installation position for installing immunochromatography detection sensor, and has
There is detection zone；The pulse excitation light source is located at the both sides of the detection zone, the pulse excitation light with the fluorescence detector
Source is used to send out pulse excitation light to the immunochromatography detection sensor positioned at the detection zone, and the fluorescence detector is for connecing
Receive and detect the fluorescence sent out from the immunochromatography detection sensor of the detection zone.
The pulse excitation light source can send out the pulse that wavelength is 350nm~490nm and swash in one of the embodiments,
It shines.
The fluorescence detector is used for the fluorescence of a length of 550nm~650nm of received wave in one of the embodiments,.
The time-resolved fluoroimmunoassay chromatography detection device further includes filter element in one of the embodiments, institute
State the side that filter element is located at the detection zone, the fluorescence filter via the filter element after by the fluorescence detector
Detection.
The fluorescence detector includes that photomultiplier and the conversion of photoelectricity strength figures calculate in one of the embodiments,
Component, for the photomultiplier for receiving the fluorescence and being converted to electric signal, the photoelectricity strength figures convert calculating group
Part connect to receive the electric signal and be digitized processing to the electric signal with the photomultiplier.
A kind of fluorescence immunity analyzer, including described in input unit, display device, controller and any of the above-described embodiment
Time-resolved fluoroimmunoassay chromatographs detection device；The controller swashs with the input unit, the display device, the pulse
Light emitting source and fluorescence detector electrical connection；The input unit is used for the wavelength of input pulse exciting light；The controller
The pulse excitation light of preset wavelength is sent out for controlling the pulse excitation light source, and it is glimmering to control the fluorescence detector detection
Light；The display device is used to show the input results of the input unit and the testing result of the fluorescence detector.
The fluorescence immunity analyzer further includes immunochromatography detection sensor in one of the embodiments, described to exempt from
It is immunochromatographydetecting detecting test strip that epidemic disease, which chromatographs detection sensor, and the corresponding detection zone is provided with detection line and nature controlling line
Microporous membrane, the fluorescence detector are at least used to detect the fluorescence sent out from the detection line and the nature controlling line.
The microporous membrane is nitrocellulose filter in one of the embodiments, and the microporous membrane is coated with fluorescence
The monoclonal of microballoon label detects antibody.
Ranging from 10 μm~100 μm of the inside aperture of the microporous membrane in one of the embodiments,.
The possibility of 1000 photon energy excitation fluorescence only has 1, remaining passes through or disperses to come out.Above-mentioned time resolution
Fluorescence immune chromatography detection device is combined with time-resolved fluorescence technology, using the decay principle of different wave length, works as pulse excitation
When light passes through immunochromatography detection sensor to reach detector, excite the Photon Decay speed of fluorescence and other photons inconsistent,
Other photons decay soon, but the speed of fluorescence is slower, are divergence expressions, therefore fluorescence detector is according to this Characteristics Detection
To fluorescence.
The time-resolved fluoroimmunoassay chromatographs detection device and fluorescence immunity analyzer and detects dress compared with traditional reflection-type
It sets, transmission beam method is combined with time-resolved fluorescence, the advantages of time-resolved fluorescence is that background signal is low, and common fluorescent is not up to
It is required to this, or even lower than traditional bounce technique effect.The time-resolved fluoroimmunoassay chromatographs detection device by by arteries and veins
Impulse light emitting source and fluorescence detector are set to the both sides of detection zone, so as to the immunochromatography detection sensor from detection zone
The fluorescence sent out is received and is detected, and relative to traditional reflection-type detection method, can not only detect immunochromatography detection
The fluorescence signal of sensor surface can also detect the fluorescence signal inside immunochromatography detection sensor, and then can carry
The signal strength of high detection is conducive to improve detection sensitivity.By test, is chromatographed and examined using above-mentioned time-resolved fluoroimmunoassay
Device and fluorescence immunity analyzer are surveyed to detect the fluorescence signal of immuno-chromatographic test paper strip, is received compared with traditional bounce technique
The signal-to-noise ratio of fluorescence signal effectively improves 10%~20%.
Description of the drawings
Fig. 1 is that the time-resolved fluoroimmunoassay of an embodiment chromatographs the structural schematic diagram of detection device；
Fig. 2 is the modular structure schematic diagram of the fluorescence immunity analyzer of an embodiment.
Specific implementation mode
The utility model is more fully retouched below with reference to relevant drawings for the ease of understanding the utility model,
It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can in many different forms come in fact
It is existing, however it is not limited to embodiment described herein.Make public affairs to the utility model on the contrary, purpose of providing these embodiments is
The understanding for opening content is more thorough and comprehensive.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.
Unless otherwise defined, all of technologies and scientific terms used here by the article is led with the technology for belonging to the utility model
The normally understood meaning of technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein "and/or" includes
Any and all combinations of one or more relevant Listed Items.
As shown in Figure 1, the time-resolved fluoroimmunoassay chromatography detection device 100 of an embodiment includes pulse excitation light source
110, sensor mounting block 120 and fluorescence detector 130.Sensor mounting block 120 has for installing immunochromatography inspection
The sensor installation position of sensor 200 is surveyed, and there is detection zone 122.Pulse excitation light source 110 is located at fluorescence detector 130
The both sides of detection zone 122.Pulse excitation light source 110 to the immunochromatography detection sensor 200 positioned at detection zone 122 for sending out
Pulse excitation light 111.Fluorescence detector 130 for receive sent out from the immunochromatography detection sensor 200 of detection zone 122 it is glimmering
Light 112.
In the present embodiment, the immunochromatography detection sensor 200 can be but not limited to immunochromatography detection examination
Paper slip.The immunochromatographydetecting detecting test strip is used to correspond to the microporous membrane for being provided with detection line and nature controlling line of detection zone 122, such as
Nitrocellulose filter (NC films) etc..The micropore that the fine structure of NC films has many apertures different, and different NC fenestras are directly gone to
Toward all differences, but these micropores, the light wave of certain wavelength can penetrate over, the easier transmission of the bigger light wave of special wavelength.
Fluorescence detector 130 is at least used to detect the fluorescence 112 sent out from detection line and nature controlling line.
The pulse excitation light source 110 of present embodiment is preferably LED light source, can also use laser light source.Pulse excitation
The wavelength for the exciting light that light source 110 is sent out is preferably 350nm~490nm, such as 365nm or 420nm.Fluorescence detector 130 is used
In the fluorescence 112 of a length of 550nm~650nm of received wave, such as the fluorescence 112 of a length of 615nm of received wave.
Time-resolved fluoroimmunoassay chromatography detection device 100 combines transmission beam method with time-resolved fluorescence technology,
The advantages of time-resolved fluorescence is that background signal is low, this requirement is not achieved in common fluorescent, or even than traditional bounce technique effect
It is lower.The time-resolved fluoroimmunoassay chromatographs detection device 100 and passes through the light wave by excitation light source for 350nm~490nm wavelength
Across the microporous membranes such as NC films, ranging from 10 μm~100 μm of the inside aperture of microporous membrane.Detection line and Quality Control on microporous membrane
The monoclonal detection antibody of the coating fluorescent microsphere label of line capture can become light wave 550nm~650nm, fluorescence detector
The light wave of 130 reception lit transmissives calculates the content of target protein.Using the time-resolved fluoroimmunoassay layer of present embodiment
The detection that detection device 100 carries out fluorescence signal is analysed, the signal-to-noise ratio for detecting the fluorescence signal on microporous membrane can be more anti-than traditional
It penetrates method and improves 10%~20%.
As shown in Figure 1, the time-resolved fluoroimmunoassay chromatography detection device 100 of present embodiment further includes being located at detection zone
The filter element 140 of 122 sides.Fluorescence 112 enters fluorescence detector 130 after filter element 140.
Further, time-resolved fluoroimmunoassay chromatography detection device 100 further includes photomultiplier 150 and photoelectricity intensity
Number conversion computation module 160.For receiving fluorescence 112 and being converted to electric signal, photoelectricity strength figures turn photomultiplier 150
Computation module 160 is changed to be connect with photomultiplier 150 for receiving electric signal and being digitized processing to electric signal.Specifically
Ground, photoelectricity strength figures conversion computation module 160 can include but is not limited to signal amplifier, analog-digital converter etc..
The time-resolved fluoroimmunoassay chromatography detection device 100 of present embodiment can not only detect immunochromatography detection and pass
The fluorescence signal on the surface of sensor 200, can also further by detect immunochromatography detection sensor 200 fluorescence 112 come
The Internal Fluorescent signal for detecting immunochromatography detection sensor 200, the fluorescence signal received so as to fluorescence detector 130 are strong
Degree.
Incorporated by reference to Fig. 1 and Fig. 2, present embodiment additionally provides a kind of fluorescence immunity analyzer 10 comprising input unit
300, the time-resolved fluoroimmunoassay of display device 400, controller 500 and any of the above-described embodiment chromatographs detection device 100.Control
Device 500 processed is electrically connected with input unit 300, display device 400, pulse excitation light source 110 and fluorescence detector 130.Input dress
Set 300 wavelength for inputting exciting light.Controller 500 is for controlling the excitation that pulse excitation light source 110 sends out preset wavelength
Light, and control fluorescence detector 130 and detect fluorescence 112.Display device 400 for display input device 300 input results and
The testing result of fluorescence detector 130.
Traditional immunofluorescence chromatography method is the sample that a certain amount of sample is added drop-wise to immunochromatographydetecting detecting test strip
On pad, moved forward by slab effect, the labelled reagent being dissolved on bonding pad is reacted with sample, when conjugate be moved to it is anti-
When original detection band, determinand occurs reacting for specificity with conjugate and is trapped, and conjugate is enriched in detection line, and conjugate contains
Amount is directly proportional to the determinand content in sample.The conjugate content in fluorescence intensity and test strips generated under excitation light source
It is directly proportional, when light source is irradiated to the detection line and nature controlling line of test strips, the fluorescent material of attachment, emission light gathering is excited simultaneously to turn
Electric signal is turned to, the power of electric signal is related to fluorescent molecular quantity, and determinand contains in fluorescence immunity analyzer calculating sample
Amount.Above-mentioned time-resolved fluoroimmunoassay chromatography detection device 100 and fluorescence immunity analyzer 10 are detected compared with traditional reflection-type
Device, by the way that pulse excitation light source 110 and fluorescence detector 130 to be set to the both sides of detection zone 122, so as to from detection
The fluorescence 112 that the immunochromatography detection sensor 200 in area 122 passes through is received and is detected, and is examined relative to traditional reflection-type
Survey method can not only detect the fluorescence signal on 200 surface of immunochromatography detection sensor, can also detect that immunochromatography is examined
The fluorescence signal inside sensor 200 is surveyed, and then the signal strength of detection can be improved, is conducive to improve detection sensitivity.Separately
Outside, it is that time-resolved fluoroimmunoassay of the invention chromatographs 100 energy of detection device with traditional different benefit of detector location
Allowing fluorescence detector 130 to send out place with fluorescence 112 can be closer, more to receive light.
Each technical characteristic of embodiment described above can be combined arbitrarily, to keep description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But therefore it can not be interpreted as the limitation to utility model patent range.It should be pointed out that for the common skill of this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The scope of protection of the utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (9)
1. a kind of time-resolved fluoroimmunoassay chromatographs detection device, which is characterized in that installed including pulse excitation light source, sensor
Component and fluorescence detector；The sensor mounting block has to be installed for installing the sensor of immunochromatography detection sensor
Position, and there is detection zone；The pulse excitation light source is located at the both sides of the detection zone, the pulse with the fluorescence detector
Excitation light source is used to send out pulse excitation light, the fluorescence detector to the immunochromatography detection sensor positioned at the detection zone
The fluorescence that immunochromatography detection sensor for receiving and detecting from the detection zone is sent out.
2. time-resolved fluoroimmunoassay as described in claim 1 chromatographs detection device, which is characterized in that the pulse excitation light
Source can send out the pulse excitation light that wavelength is 350nm~490nm.
3. time-resolved fluoroimmunoassay as described in claim 1 chromatographs detection device, which is characterized in that the fluorescence detector
Fluorescence for a length of 550nm~650nm of received wave.
4. time-resolved fluoroimmunoassay according to any one of claims 1 to 3 chromatographs detection device, which is characterized in that also
Including filter element, the filter element is located at the side of the detection zone, after the fluorescence filters via the filter element
It is detected by the fluorescence detector.
5. time-resolved fluoroimmunoassay according to any one of claims 1 to 3 chromatographs detection device, which is characterized in that institute
It includes photomultiplier and photoelectricity strength figures conversion computation module to state fluorescence detector, and the photomultiplier is for receiving institute
It states fluorescence and is converted to electric signal, the photoelectricity strength figures conversion computation module is connect with the photomultiplier for connecing
It receives the electric signal and processing is digitized to the electric signal.
6. a kind of fluorescence immunity analyzer, which is characterized in that including input unit, display device, controller and such as claim 1
Time-resolved fluoroimmunoassay described in any one of~5 chromatographs detection device；It is the controller and the input unit, described aobvious
Showing device, the pulse excitation light source and fluorescence detector electrical connection；The input unit is used for input pulse exciting light
Wavelength；The controller is used to control the pulse excitation light that the pulse excitation light source sends out preset wavelength, and described in control
Fluorescence detector detects fluorescence；The display device is for showing the input results of the input unit and the fluorescence detector
Testing result.
7. fluorescence immunity analyzer as claimed in claim 6, which is characterized in that further include immunochromatography detection sensor, institute
To state immunochromatography detection sensor be immunochromatographydetecting detecting test strip, and the corresponding detection zone is provided with detection line and Quality Control
The microporous membrane of line, the fluorescence detector are at least used to detect the fluorescence sent out from the detection line and the nature controlling line.
8. fluorescence immunity analyzer as claimed in claim 7, which is characterized in that the microporous membrane is nitrocellulose filter,
The microporous membrane is coated with the monoclonal detection antibody of fluorescent microsphere label.
9. fluorescence immunity analyzer as claimed in claim 8, which is characterized in that the inside aperture of the microporous membrane is ranging from
10 μm~100 μm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109596838A (en) * | 2018-12-17 | 2019-04-09 | 中国科学院电子学研究所 | The unicellular interior multiple protein simultaneous quantitative detection system of one kind and method |
| CN113075175A (en) * | 2021-03-15 | 2021-07-06 | 中国科学院福建物质结构研究所 | Broadband time-resolved fluorescence immunoassay device and analysis method |
-
2018
- 2018-01-29 CN CN201820154446.3U patent/CN207866710U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109596838A (en) * | 2018-12-17 | 2019-04-09 | 中国科学院电子学研究所 | The unicellular interior multiple protein simultaneous quantitative detection system of one kind and method |
| CN113075175A (en) * | 2021-03-15 | 2021-07-06 | 中国科学院福建物质结构研究所 | Broadband time-resolved fluorescence immunoassay device and analysis method |
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Address after: No.79, Ruihe Road, Science City, Guangzhou hi tech Industrial Development Zone, Guangdong 510000 Patentee after: Reboo (Guangzhou) Biotechnology Co.,Ltd. Address before: 510530 No. 79 Ruihe Road, Luogang District, Guangzhou City, Guangdong Province Patentee before: RAYBIOTECH, Inc. |