CN208568797U - A kind of detection capsaicine time-resolved fluoroimmunoassay quantifies POCT kit - Google Patents
A kind of detection capsaicine time-resolved fluoroimmunoassay quantifies POCT kit Download PDFInfo
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- CN208568797U CN208568797U CN201820966815.9U CN201820966815U CN208568797U CN 208568797 U CN208568797 U CN 208568797U CN 201820966815 U CN201820966815 U CN 201820966815U CN 208568797 U CN208568797 U CN 208568797U
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Abstract
The utility model discloses a kind of detection capsaicine time-resolved fluoroimmunoassay to quantify POCT kit, including box cover, cassette bottom, inner box plate, mounting bracket, several extracting solution bottles, several dilution bottles, several reagent strips, ID card and bar code, box cover is hinged by hinged shaft with cassette bottom back edge, inner box plate is hinged with the side of box cover and cassette bottom respectively by the first strut and the second strut, it is fixed sandwich structure in inner box plate, inner box edges of boards are along with extraction aperture, reagent strip arrangement is inserted into inner box plate, mounting bracket is mounted on inside cassette bottom, multiple liquid bottle mounting holes are offered thereon, extracting solution bottle and dilution bottle are inserted into liquid bottle mounting hole, ID sticker is attached in box cover, bar code is attached on the outside of box cover.The utility model using double-layer structure by the space of kit rationally using and conveniently take reagent strip, while reagent strip being effectively prevent to pollute, high sensitivity, high specificity, the range of linearity are wide, easy to operate.
Description
Technical field
The utility model relates to time-resolved fluoroimmunoassay quantitative measurement technology fields, and in particular to a kind of detection capsaicine
Time-resolved fluoroimmunoassay quantifies POCT kit.
Background technique
Capsaicine chemical name is called trans- 8- methy-N-vanillyl -6- nonenamide, is the active constituent of capsicum, and
Make capsicum that there is the main compound of pungent stimulation.Capsaicine also has and disappears in addition to assigning food acid and being used as flavouring
Inflammation analgesic, antibacterial, antitumor and gastric acid secretion inhibiting have the medical values such as protective effect to mucosal lesion.It is led in pesticide
Domain, capsaicine substance are considered as a kind of ideal public nuisance-free agricultural chemicals, for the anti-of the crop disease and insects such as water fruits and vegetables, cereal
It controls.It can be used for high-grade special antifouling paint in light industry field, or prevent mouse from biting in cable material.Therefore, it examines
The content for surveying capsaicine is significant to Capsaicinoids Normalization rule and safety evaluation.
It is mainly at present instrumental method, high performance liquid chromatography or LC-MS, instrument for the detection method of capsaicine
Device analytic approach needs large-scale instrument and equipment, and pre-treatment is cumbersome time-consuming, and testing cost is high, it is difficult to meet quick, on-line checking need
It asks.Therefore, it is proposed that it is a kind of both easy to be quick, it is inexpensive, and the time-resolved fluorescence of the detection capsaicine of energy accurate quantitative analysis
It is i.e. quickly square can to meet scene to detect the content of capsaicine in various samples for immune quantitative POCT detection kit and method
Just the requirement of accurate quantitative analysis detection again.
Meanwhile the open placement in kit of test agents useful for same item is detected, it is easy to pollution or causes inside kit
Confusion, another way are that reagent strip is held by aluminium foil bag, need to tear aluminium foil bag when needing, and high-volume, multisample are examined
When survey, unpacks waste time one by one.Existing detection capsaicine equipment price is expensive, complicated for operation, detection time is long, Wu Faxian
Field detecting and accuracy and less reproducible, cannot quantify, be easy to cause the difficulty of false positive or false negative.
Utility model content
The purpose of the utility model is to provide it is a kind of can it is easy quickly, capsaicin content in the test sample of accurate quantitative analysis
Capsaicine time-resolved fluoroimmunoassay quantify POCT kit.
The utility model in order to achieve the above objectives, is achieved especially by following technical scheme:
A kind of detection capsaicine time-resolved fluoroimmunoassay quantifies POCT kit, including box cover, cassette bottom, inner box plate, solid
Determine bracket, several extracting solution bottles, several dilution bottles, several reagent strips, ID card and bar code, box cover to pass through with cassette bottom back edge
Hinged shaft is hinged, and inner box plate is hinged with the side of box cover and cassette bottom respectively by the first strut and the second strut, is solid in inner box plate
Determine sandwich, inner box edges of boards are along having extraction aperture, and reagent strip arrangement is inserted into inner box plate, and mounting bracket is mounted in cassette bottom
Portion offers multiple liquid bottle mounting holes thereon, and extracting solution bottle and dilution bottle are inserted into liquid bottle mounting hole, and ID sticker is attached to box
In lid, bar code is attached on the outside of box cover.
Further, reagent strip includes that plastics get stuck, plastics get stuck intraformational bed overlap joint be placed with sample pad, bonding pad, nitre
Acid cellulose film and absorption pad are adsorbed with the capsaicine antibody and rabbit igg marked with time-resolved fluorescence microballoon on bonding pad
Antibody successively has detection T line, Quality Control C line on nitrocellulose filter, detects and is embedded with capsaicine antigen, Quality Control C line on T line
On be embedded with goat-anti rabbit secondary antibody.
Further, reagent strip is provided with anti-skid structure close to one end upper surface of extraction aperture.
Further, the lock and lock tongue to match is respectively arranged on the outside of box cover and cassette bottom.
Further, first strut one end is hinged on the side rear portion of inner box plate, and it is separate that the other end is hinged on box cover side
The position of hinged shaft.
Further, second strut one end is hinged on the side front of inner box plate, and it is close that the other end is hinged on cassette bottom side
The position of hinged shaft.
Further, handle is provided on the outside of box cover.
Compared with prior art, the utility model using double-layer structure by the space of kit rationally using and conveniently take
Reagent strip, while reagent strip being effectively prevent to pollute；By the high degree of specificity of immune response and unique fluorescent microsphere as anti-
Body trace labelling object, and combined with highly sensitive time-resolved fluoroimmunoassay chromatographic technique and POCT theory, propose one
Kind of on-radiation, high sensitivity, high specificity, the range of linearity be wide, simple and quick new detection method.
Detailed description of the invention
Fig. 1 is the overall structure diagram of the utility model；
Fig. 2 is the diagrammatic cross-section of reagent strip in the utility model；
Fig. 3 is the overall structure diagram of reagent strip in the utility model.
In figure, 1, box cover；11, it latches；2, cassette bottom；21, lock tongue；3, inner box plate；31, the first strut；32, the second strut；
33, extraction aperture；4, mounting bracket；41, liquid bottle mounting hole；5, extracting solution bottle；6, dilution bottle；7, reagent strip；71, plastic clip
Shell；72, sample pad；73, bonding pad；74, nitrocellulose filter；741, T line is detected；742, Quality Control C line；75, absorption pad；76,
Anti-skid structure；8, ID card；9, bar code；10, hinged shaft.
Specific embodiment
The utility model is described in further detail with reference to the accompanying drawings and embodiments.
As shown in Figure 1 to Figure 3, a kind of detection capsaicine time-resolved fluoroimmunoassay of the utility model quantifies POCT reagent
Box, box cover 1, cassette bottom 2, inner box plate 3, mounting bracket 4, several extracting solution bottles 5, several dilution bottles 6, several reagent strips 7, ID card
8 and bar code 9, box cover 1 and 2 back edge of cassette bottom are hinged by hinged shaft 10, and inner box plate 3 passes through the first strut 31 and the second strut 32
It is hinged with box cover 1 and the side of cassette bottom 2 respectively, it is preferable that 31 one end of the first strut is hinged on the side rear portion of inner box plate 3, separately
One end is hinged on position of 1 side of box cover far from hinged shaft 10；Second strut, 32 one end is hinged on the side front of inner box plate 3, separately
One end is hinged on 2 side of cassette bottom close to the position of hinged shaft 10.Box cover 1 and the movable fastening of cassette bottom 2, box cover 1 form examination after fastening
Inner box plate 3 is accommodated in the inner space of kit by the inner space of agent box, opens the lid 1, and inner box plate 3 passes through the first support
The activity of the connecting rod of bar 31 and the second strut 32, inner box plate 3 and cassette bottom 2 are separated, and expose the inner space of cassette bottom 2.Preferably, box
It is respectively arranged with the lock and lock tongue to match on the outside of lid 1 and cassette bottom 2, box cover 1 and cassette bottom 2 are fastened, prevent from pouring down or forth.Box
Handle is provided on the outside of lid 1.
Inner box plate 3 is fixed sandwich structure, and 3 edge of inner box plate has extraction aperture 33, and the arrangement of reagent strip 7 is inserted into inner box plate
In 3.To avoid reagent strip 7 from directly contacting with the external world, prevents from polluting, and can take when detecting test conveniently, save test
Operation, by all reagent strips 7 arrangement inserting in middle level the inner box plate 3 of inner box plate 3, inner box plate 3 can be by of different size up and down
Two Boards walls are formed, the two clamping plate self-assembling formation extraction apertures 33 stacked.Different reagent strips 7 can take out from extraction aperture 33.It is preferred that
Ground, reagent strip 7 are provided with anti-skid structure 76 close to one end upper surface of extraction aperture 33, are convenient for manual withdrawal.
Mounting bracket 4 is mounted on inside cassette bottom 2, offers multiple liquid bottle mounting holes 41, extracting solution bottle 5 and dilution thereon
Bottle 6 is inserted into liquid bottle mounting hole 41.The institute that the quantity and amount of reagent of extracting solution bottle 5 and dilution bottle 6 detect for single test
It needs reagent proportion to determine, test of many times detection amount used can be stored in a kit.The detection capsaicine time of the utility model
The extracting solution of extracting solution bottle is methanol solution in resolved fluorometric immune quantitative POCT kit.The dilution of dilution bottle be containing
0.5% (m/v) bovine serum albumin(BSA), the phosphate buffer solution of 0.01mol/L, pH7.4 of 1% (v/v) tween-20.
ID card 8 is attached in box cover 1, and bar code 9 is attached to 1 outside of box cover.ID card 8 and bar code 9 are for scanning display
The identity informations such as the batch and performance of kit, and with standard curve and detection project information, be used to automatic identification and detect
Project and the standard curve information for extracting this batch kit.Specification is accompanied by kit, specification is testing principle, behaviour
Make the detailed description of step, points for attention.ID card and bar code are used to automatic identification with standard curve and detection project information
Detection project and the standard curve information for extracting this batch kit.
Preferably, reagent strip 7 includes that plastics get stuck 71, plastics get stuck 71 intraformational beds be placed with sample pad 72, bonding pad 73,
Nitrocellulose filter 74 and absorption pad 75 are adsorbed with the capsaicine antibody marked with time-resolved fluorescence microballoon on bonding pad 73
With rabbit igg antibody, successively there is on nitrocellulose filter 74 detection T line 741, Quality Control C line 742, detect and be embedded on T line 741
Capsaicine antigen, the goat-anti rabbit secondary antibody embedded on Quality Control C line 742.
The assembling of capsaicine fluorescence immunoassay quantitative detecting reagent item, prepares capsaicine time-resolved fluoroimmunoassay quantitative detection
Reagent strip is to obtain capsaicine artificial antigen and the monoclonal antibody that animal obtains is immunized using artificial antigen first, real herein
Capsaicine artificial antigen and corresponding monoclonal antibody used in new patent are all obtained by commercialization.When its capsaicine
Between the assembly program of resolved fluorometric immune quantitative detection reagent item be first to mark capsaicine with time resolution europium (Eu) fluorescent microsphere
Then antibody is dried and is fixed on bonding pad, in combination be also sprayed on pad 72 time resolution Eu fluorescent microsphere label
Rabbit igg antibody, then capsaicine antigen is fixed on nitrocellulose filter and is made into detection T line 741, while in nitrocellulose filter
It is coated with goat-anti rabbit secondary antibody on 74 and is made into 742,37 DEG C of Quality Control C line drying, finally by sample pad 72, bonding pad 73, nitrocellulose
Film 74 and absorption pad 75 are successively overlapped in PVC board, then are cut into the reagent strip of 4mm wide.It is transferred to PVC plastic to get stuck in 71, plastics
71 are got stuck equipped with the well matched with sample pad 72, and matched with reaction film detection T line 741 and Quality Control C line 742
Fluorescence reading window.
Capsaicine time-resolved fluoroimmunoassay quantitative detecting reagent test philosophy: it is added dropwise when by sample to be tested in sample application zone
When, the capsaicine in sample is in conjunction with the capsaicine antibody that fluorescent microsphere in bonding pad 73 marks and through capillary action to front layer
Analysis after reaching detection zone, detects capsaicine antigen fixed on T line 741 and remaining unbonded fluorescent microsphere marks capsaicine
Antibody combines.Detection T line 741 on combine fluorescent microsphere label capsaicine antibody concentration and sample in capsaicine concentration at
Inverse ratio, the fluorescent marker concentration that Quality Control C line 742 combines are unrelated with the concentration of capsaicine in sample.After chromatography, when use
Between resolved fluorometric immune quantitative POCT analyzer read detection T line 741 fluorescence intensity and Quality Control C line 742 relative fluorescence it is strong
T/C value is spent and calculates, by, with the ID card and bar code of standard curve and detection project information, using time resolution in kit
Fluorescence immunoassay quantifies the concentration that the analysis software built in POCT analyzer calculates capsaicine in sample automatically.
The utility model quantifies POCT detection reagent item by constructing capsaicine time-resolved fluoroimmunoassay, and utilizes the time
Resolved fluorometric immune quantitative POCT detection kit, using the principle of competitive immunoreaction, only with unification platform time-resolved fluorescence
Immune quantitative POCT analyzer, it can be achieved that in convenient and efficient, inexpensive, highly sensitive quantitative detection sample capsaicine content.
Fluorescent marker is to wrap up the fluorescent microsphere being process through special using lanthanide series Eu in reagent strip 7, and fluorescence intensity ratio is general
Logical marker wants high 100 times or more, according to its luminous characteristics and spectral characteristic, measures transmitting fluorescence with TIME RESOLVED TECHNIQUE, leads to
Time delay and wavelength resolution are crossed, the interference of non-specific fluorescence is effectively excluded, greatly improves sensitivity for analysis.With this reality
Sensitivity with novel kit method detection capsaicine is 0.05 μ g/kg, and the range of linearity is 0.2-10.00 μ g/kg, accurately
Degree TIANZHU XINGNAO Capsul is 80-120%, and 5 minutes accurate quantitative analysis of sample solution determine the content of capsaicine.
The fluorescence immunoassay that reagent solution is all contained in the utility model quantifies in POCT kit, without in addition preparing examination
Agent.
Instrument and equipment: XT8201A type time-resolved fluoroimmunoassay quantifies POCT analyzer, and (Shanghai ambitious plan biotechnology has
Limit company provides), XT8202A type constant-temperature incubation device, eddy blending machine, low speed centrifuge, electronic balance, pulverizer and homogeneous
Machine.
Test method:
1. the pre-treatment of sample
By about 40 mesh of sample comminution, after mixing, 2.0 ± 0.02g sample is accurately weighed in 15ml centrifuge tube, and 10ml is added
Sample extracting solution, with vortex oscillator mechanical shaking extraction 5min；After extraction, 4000rpm is centrifuged 2min, takes 100 μ l supernatants, adds
Enter to 200 μ l sample diluting liquids, to sample detection after mixing.
2. detection process and result
1) constant-temperature incubation device is switched on, is arranged 37 DEG C of temperature, rising to 37 DEG C of constant rears to temperature can be used；2) fluorescence is exempted from
After epidemic disease quantifies POCT analyzer preheating 5min, it is inserted into ID card corresponding to this batch products, and scan corresponding to this batch products
Bar code；3) reagent strip 7 is extracted from inner box plate 3, is horizontally placed on constant-temperature incubation device；4) 100 μ l are drawn with pipettor
Sample to be tested by pre-treatment is added in the well of reagent strip 7, covers couveuse lid, starts timing and is incubated for 5min；5)
The insertion fluorescence immunoassay of reagent strip 7 is quantified in POCT analyzer reagent slot after 5min, is read by read key or touch screen point
, reading value is the content of capsaicine in sample.If testing result exceeds the upper limit of the range of linearity, diluted with sample
Liquid detects again after diluting 5 times, and testing result is multiplied by 5, the as ultimate density of sample.
The specific embodiment of the utility model is only the explanation to the utility model, is not to the utility model
Limitation, those skilled in the art can according to need after reading this specification makes without creative tribute the present embodiment
The modification offered, but as long as all by the protection of Patent Law in the scope of the claims of the utility model.
Claims (6)
1. a kind of detection capsaicine time-resolved fluoroimmunoassay quantifies POCT kit, which is characterized in that including box cover (1), box
Bottom (2), inner box plate (3), mounting bracket (4), several extracting solution bottles (5), several dilution bottles (6), several reagent strips (7), ID
Block (8) and bar code (9), hingedly by hinged shaft (10), inner box plate passes through the first strut (31) and for box cover and cassette bottom back edge
Two struts (32) are hinged with the side of box cover and cassette bottom respectively, and inner box plate is fixed sandwich, and inner box edges of boards are along with extraction aperture
(33), reagent strip arrangement is inserted into inner box plate, and mounting bracket is mounted on inside cassette bottom, offers multiple liquid bottle mounting holes thereon
(41), extracting solution bottle and dilution bottle are inserted into liquid bottle mounting hole, and ID sticker is attached in box cover, and bar code is attached to outside box cover
Side；Reagent strip includes that plastics get stuck (71), plastics get stuck intraformational bed overlap joint be placed with sample pad (72), bonding pad (73), nitric acid
Cellulose membrane (74) and absorption pad (75), be adsorbed on bonding pad the capsaicine antibody marked with time-resolved fluorescence microballoon and
Rabbit igg antibody successively has detection T line (741), Quality Control C line (742) on nitrocellulose filter (74), detects and be embedded on T line
Capsaicine antigen is embedded with goat-anti rabbit secondary antibody on Quality Control C line.
2. detection capsaicine time-resolved fluoroimmunoassay according to claim 1 quantifies POCT kit, which is characterized in that
Reagent strip is provided with anti-skid structure (76) close to one end upper surface of extraction aperture.
3. detection capsaicine time-resolved fluoroimmunoassay according to claim 1 quantifies POCT kit, which is characterized in that
The lock (11) and lock tongue (21) to match is respectively arranged on the outside of box cover and cassette bottom.
4. detection capsaicine time-resolved fluoroimmunoassay according to claim 1 quantifies POCT kit, which is characterized in that
First strut (31) one end is hinged on the side rear portion of inner box plate, and the other end is hinged on position of the box cover side far from hinged shaft.
5. detection capsaicine time-resolved fluoroimmunoassay according to claim 1 quantifies POCT kit, which is characterized in that
Second strut (32) one end is hinged on the side front of inner box plate, and the other end is hinged on cassette bottom side close to the position of hinged shaft.
6. detection capsaicine time-resolved fluoroimmunoassay according to claim 1 quantifies POCT kit, which is characterized in that
Handle is provided on the outside of box cover.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820966815.9U CN208568797U (en) | 2018-06-22 | 2018-06-22 | A kind of detection capsaicine time-resolved fluoroimmunoassay quantifies POCT kit |
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| CN201820966815.9U CN208568797U (en) | 2018-06-22 | 2018-06-22 | A kind of detection capsaicine time-resolved fluoroimmunoassay quantifies POCT kit |
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| CN208568797U true CN208568797U (en) | 2019-03-01 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115078713A (en) * | 2022-05-16 | 2022-09-20 | 远大医药（中国）有限公司 | Kit, credible detection result acquisition method and anti-counterfeiting test paper |
-
2018
- 2018-06-22 CN CN201820966815.9U patent/CN208568797U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115078713A (en) * | 2022-05-16 | 2022-09-20 | 远大医药（中国）有限公司 | Kit, credible detection result acquisition method and anti-counterfeiting test paper |
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