CN1053837C - Micro-molecule glucoprotein biologics and its preparation - Google Patents
Micro-molecule glucoprotein biologics and its preparation Download PDFInfo
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- CN1053837C CN1053837C CN92114257A CN92114257A CN1053837C CN 1053837 C CN1053837 C CN 1053837C CN 92114257 A CN92114257 A CN 92114257A CN 92114257 A CN92114257 A CN 92114257A CN 1053837 C CN1053837 C CN 1053837C
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Abstract
The present invention relates to a medicinal preparation containing a peptide material, and a preparation method for the medicinal preparation, particularly to a micromolecule glucoprotein biological preparation and a preparation method thereof, which is characterized in that the medicinal preparation is prepared by that health human early embryo tissue after being processed through artificial abortion is adopted as a raw material and processed through the procedures of mashing, cracking by freeze thawing, dialysis, etc. Compared with the prior art, the present invention has the following advantages of high bioactivity, wide treatment range, no toxic or side effect, no pyrogenic reaction or allergic reaction, and low manufacturing cost. The medicinal preparation has obvious function of promoting antibody forming cells, has a predominant therapeutic effect on rheumatoid arthritis, bronchial asthma and anaphylactic diseases, and a total effective rate reaches up to higher than 90%.
Description
The present invention relates to a kind of medicinal preparation that contains peptide class material and preparation method thereof, particularly a kind of small molecule glycoprotein biological agent and preparation method thereof.
In the prior art to the medicine of treatment rheumatoid arthrosis class toxic side effect all, Radix Tripterygii Wilfordii etc. for example, the patient can not adhere to life-time service, the patient who has even be forced to therapy discontinued.
Small molecule glycoprotein biological agent of the prior art is that to adopt blood of human body be raw material, for example Chang Yong transfer factor [N-TF], and it is isolated from blood of human body exactly from cell, behind 4～5 freezing-thawing and crackings, gives small-molecular peptides.This prior art has following several weak point: biological activity is not high; It is not obvious that antagonist forms the cell facilitation; Therapeutic domain is narrow, and is not good to some immune diseases and anaphylaxis eqpidemic disease therapeutic effect, for example, and rheumatoid arthrosis class, bronchial asthma etc.; The cost of material height, so the preparation cost is more expensive, the scale that has restricted preparation from economic angle is used.
The objective of the invention is to overcome weak point of the prior art, and a kind of biological activity height is provided, it is obvious that antagonist forms the cell facilitation, therapeutic domain is wide, have no side effect, raw material sources are extensive, the small molecule glycoprotein biological agent that cost of manufacture is low and make each method.
The objective of the invention is to realize by following approach.
Small molecule glycoprotein biological agent, is made after freezing-thawing and cracking and the dialysis through separating by biological tissue of human body, and said biological tissue of human body is a kind of healthy human body body early embryo tissue of obtaining after artificial abortion.
Maximum molecular weight Mw20000 in the said biological preparation, OD
260/ OD
280＞2, OD260 represents the absorbance of said biological preparation under wavelength 260nm, OD in the formula
280Represent the absorbance of said biological preparation under wavelength 280nm.
Said biological preparation molecular weight index is preferably: best OD in the total said biological preparation of weight average molecular weight Mw6337.47 of maximum molecular weight Mw12551.70 minimum molecular weight Mw1031.82
360/ OD
380=2.7 ± 0.1.
Experiment showed, in the biological preparation molecular weight at the part ubiquity below 12000 to antibacterial, the immunity of virus; Molecular weight has nonspecific immunological enhancement and immune induction effect less than 3500 part.
Said biological preparation is every milliliter of nitrogen content 10 ± 1mg preferably.
The small molecule glycoprotein biological agent preparation method, getting biological tissue of human body is raw material, dialyses behind freezing-thawing and cracking again, said raw material is the healthy human body body early embryo tissue of obtaining artificial post-abortion, its preparation process is as follows, removes impurity, smashs to pieces and makes tissue homogenate, said embryonal tissue is put into tissue mashing machine, smash rotating speed to pieces to low 8000 rev/mins, smash number of times to pieces at least 8 times, smash 50 seconds～100 seconds time at every turn to pieces, freeze thawing at least 3 times was dialysed 36～48 hours.
Smash rotating speed to pieces and be preferably 12000 rev/mins, smash number of times to pieces and be preferably 12 times, the time of smashing to pieces is preferably 60 seconds at every turn.
Said freeze thawing, cryogenic temperature are preferably-15 ℃, and melt temperature is preferably 37 ℃, and number of freezing and thawing is preferably 5 times.
In sum, the present invention has following advantage compared to existing technology: contain 18 seed amino acids, wherein cystine is up to 2.98mg/100ml, and modern medicine shows that cystine has very strong capability of resistance to radiation; Be rich in the trace element that the multiple human body utmost point needs, wherein ferrum Fe, copper Cu, manganese Mn are respectively 0.14 mcg/ml, 0.018 mcg/ml, 0.02 mcg/ml, and ferrum can promote the human body hemoposieis; The biological activity height, it is obvious that antagonist forms the cell facilitation.Following table is represented the present invention and transfer factor [N-TF], and the micromolecule thymic factor relatively uses, the antibody forming cell's parameter that is manifested, and reagent dosage all by the amount conversion course of treatment, calculate by 10 times of amounts that the mice consumption is behaved,
Therapeutic domain is wide, and clinical trial shows that the present invention is to treatment bronchial asthma, and rheumatoid arthrosis class, skin allergic disease etc. have significant curative effect, total effective rate to reach more than 90%; Have no side effect, appearance colorless of the present invention is transparent, and the apyrogeneity reaction does not have irritated reaction; Raw material sources are extensive, and are the embryonal tissues that makes full use of after the artificial abortion dilatation and curettage, cost of material need not take place, and because processing technology is simple, utilize hospital's test chamber existing equipment to prepare, and so the product cost is low, are convenient to popularization.
| The preparation title | Mus number (only) | Antibody forming cell's (spleen) |
| Small molecule glycoprotein biological agent | 6 | 125264±25599 |
| Transfer factor | 6 | 58385±15023 |
| The micromolecule thymic factor | 6 | 51783±7786 |
Below we carry out more detailed description by embodiment to the present invention.Embodiment:
Get the healthy human body body early embryo tissue of obtaining after the artificial abortion, clean, after removing impurity, put into temperature control multi-purpose high speed tissue mashing machine, under 12000 rev/mins of rotating speeds, smash to pieces 12 times, the time of smashing to pieces is 60 seconds at every turn, like this, after making tissue homogenate, put into cryogenic refrigerator, in-15 ℃ freezing thoroughly after, take out, be placed in the electric-heated thermostatic water bath, under+37 ℃ of temperature, melt thoroughly, send in the cryogenic refrigerator freezing again, 5 times so repeatedly, the gained tissue homogenate with after the plastic sheeting wrapping, is soaked in the beaker that distilled water is housed, beaker is placed on+4 ℃ of refrigerators in, so, the small-molecule substance of dialysis in distilled water is small molecule glycoprotein biological agent of the present invention.Its every leading indicator is as follows: maximum molecular weight Mw12551.7, and minimum molecular weight Mw1031.82, total weight average molecular weight Mw6337.07, ultraviolet spectra: maximum absorption band is 236nm,
The minimal absorption peak is 320nm, OD
260/ OD
280=2.7 ± 0.1, every milliliter of nitrogen content 10 ± 1mg, contain 18 seed amino acids, wherein cystine is 2.96 milligrams/100 milliliters, and it is as follows to contain the trace element index: (unit mcg/ml) Na K Ca Mg Zn Cu Fe Mn47.6 64.7 4.01 3.50 0.33 0.018 0.14 0.02.
Claims (3)
1. small molecule glycoprotein biological agent preparation method, getting biological tissue of human body is raw material, dialyses behind freezing-thawing and cracking again, it is characterized in that,
(1) said raw material is the healthy human body body early embryo tissue of obtaining artificial post-abortion,
(2) its preparation process is as follows,
(2.1) remove impurity,
(2.2) smash to pieces and make tissue homogenate,
(2.2.1) said embryonal tissue is put into tissue mashing machine,
(2.2.2) smash rotating speed to pieces to low 8000 rev/mins,
(2.2.3) smash number of times to pieces at least 8 times,
(2.2.4) smash to pieces 50 seconds-100 seconds time at every turn,
(2.3) freeze thawing is at least 3 times,
(2.4) dialysis is 36-48 hour.
2. small molecule glycoprotein biological agent preparation method according to claim 1 is characterized in that,
(1) smashing rotating speed to pieces is 12000 rev/mins
(2) smashing number of times to pieces is 12 times,
(3) time of smashing to pieces is 60 seconds at every turn.
3. small molecule glycoprotein biological agent preparation method according to claim 1 is characterized in that, said freeze thawing,
(1) cryogenic temperature is-15 ℃,
(2) melt temperature is 37 ℃,
(3) number of freezing and thawing is 5 times
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92114257A CN1053837C (en) | 1992-12-04 | 1992-12-04 | Micro-molecule glucoprotein biologics and its preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92114257A CN1053837C (en) | 1992-12-04 | 1992-12-04 | Micro-molecule glucoprotein biologics and its preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1074133A CN1074133A (en) | 1993-07-14 |
| CN1053837C true CN1053837C (en) | 2000-06-28 |
Family
ID=4946861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN92114257A Expired - Fee Related CN1053837C (en) | 1992-12-04 | 1992-12-04 | Micro-molecule glucoprotein biologics and its preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1053837C (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4888172A (en) * | 1982-09-23 | 1989-12-19 | Alfaceu Corporation | Pharmaceutical for treating tumors and methods for making it |
| US4908206A (en) * | 1986-09-16 | 1990-03-13 | Amics Ag | Extracts of embryonic organs, process for their preparation and pharmaceutical preparation containing them |
-
1992
- 1992-12-04 CN CN92114257A patent/CN1053837C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4888172A (en) * | 1982-09-23 | 1989-12-19 | Alfaceu Corporation | Pharmaceutical for treating tumors and methods for making it |
| US4908206A (en) * | 1986-09-16 | 1990-03-13 | Amics Ag | Extracts of embryonic organs, process for their preparation and pharmaceutical preparation containing them |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1074133A (en) | 1993-07-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C15 | Extension of patent right duration from 15 to 20 years for appl. with date before 31.12.1992 and still valid on 11.12.2001 (patent law change 1993) | ||
| OR01 | Other related matters | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20000628 Termination date: 20100104 |