CN105368700A - Microbe in-situ cultivation device and method used in pit - Google Patents
Microbe in-situ cultivation device and method used in pit Download PDFInfo
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- CN105368700A CN105368700A CN201510931179.7A CN201510931179A CN105368700A CN 105368700 A CN105368700 A CN 105368700A CN 201510931179 A CN201510931179 A CN 201510931179A CN 105368700 A CN105368700 A CN 105368700A
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- situ
- microorganism
- pond
- cellar
- groove
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
- C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Abstract
The invention discloses a microbe in-situ cultivation device and a microbe in-situ cultivation method used in a pit. The microbe in-situ cultivation device comprises a tank and a pressing plate, wherein at least one groove is arranged on the tank, the pressing plate is detachably mounted on the tank on the top of the groove, the tank and/or the pressing plate are/is provided with at least one through hole, and the through hole communicates the groove with the outside. A culture dish is placed in the groove and fixed through the pressing plate, the microbe in-situ cultivation device is directly placed in the pit, and the through hole communicates fermented grains and the culture dish, so that the microbe in-situ cultivation test is carried out in the pit. In order to facilitate the operation, at least two tanks are connected to form a long cultivation device, and two ends of the microbe in-situ cultivation device are respectively provided with a plug and a handle. The microbe in-situ cultivation device and the microbe in-situ cultivation method are suitable for the microbe in-situ cultivation test in the fermentation pit of the Luzhou-flavor liquor, microbes are cultivated in the natural fermentation process in the pit, the cultivation time and position can be determined independently, and the microbe in-situ cultivation device is simple in structure and convenient to operate.
Description
Technical field
The present invention relates to a kind of Culture in situ device and method being applicable to aromatic Chinese spirit fermentation pit microorganism.
Background technology
As everyone knows, brewageing of aromatic Chinese spirit belongs to open-sky technique, composite bacteria fermentation.In brewed spirit process, in environment, a large amount of microorganism enters fermentation system, carries out complicated substance metabolism, energy transformation and event information transmission at solid, liquid, gas phase interface.Through long-term domestication, microorganism has adapted to the extreme yeasting such as anoxic, high osmotic pressure, low pH, and define specific white wine microbial population, white wine microorganism interknits, interacts in fermentation system, collaborative ferment altogether, antagonism competition, decide the quality product of white wine.The research of white wine microorganism has very important meaning to understanding brewed spirit mechanism, lifting white wine quality.
At present, scientific research personnel generally adopts microorganism pure culture technigne, usually cultivate under microorganism being limited to the condition of the parameter constant such as specific nutraceutical matrix, temperature, pH, this artificial culture pattern often to cause between microorganism that the Natural Survival mode of collaborate is originally collapsed, the interruption of the extreme nutrition change in habitat, active substance and communication and ecological niche great change etc., causes microbial drug tolerance low.The limitation of pure culture technigne becomes the bottleneck of researching white spirit microbial diversity, and its result of study can not reflect the present situation of white wine microbial diversity comprehensively, and the Microbial resources in a large number with very big using value are buried.
In recent years, researchist recognizes the more educable factors of restriction micro-organisms, and has explored the novel method of some culturing micro-organisms.2002, Kaeberlein etc. devise diffusion chamber culture method, the film of diffusion chamber can make environment composition exchange in box and between environment, and the colony number that can turn out, higher than control group about 300 times, is also separated to two kinds and thought not educable microorganism in the past.This method goes up simulating natural environment largely, because the somatomedin affecting microorganism growth can pass freely through film, ensure that effect between microflora and exchanges, thus improve the Culturability of microorganism.2005, in microorganism journal 05 phase in 2005 " near-nature forest pure culture method is to the preliminary study of a microbial culture " literary composition, Ye Jiangyu etc. devise a kind of porose culture dish, are coated with not allow bacterium to pass through but nutritive substance can free-pouring millipore filtration in culture dish.During cultivation, this culture dish is put into habitat needed for separated microorganism, for being provided the possibility that same primitive environment is linked up by culturing bacterium, make the part bacterium of this ecological relationship of strong depend-ence likely be cultivated out, overcome the defect that traditional pure culture is difficult to provide external source activity material.
At present, there is no device pond, liquor fermentation cellar for storing things microorganism being carried out to Culture in situ.
Summary of the invention
Technical problem to be solved by this invention is to provide pond, a kind of cellar for storing things microorganism in situ culture apparatus.
The technical solution adopted for the present invention to solve the technical problems is: pond, cellar for storing things microorganism in situ culture apparatus, comprise cell body and pressing plate, described cell body is at least arranged a groove, the top of groove arranges described pressing plate, described pressing plate is removably installed on the cell body at the top of groove, described cell body and/or pressing plate at least arrange a through hole, and groove is in communication with the outside by described through hole.
Be positioned in groove by culture dish, then fixed by pressing plate, be directly positioned over by pond, cellar for storing things microorganism in situ culture apparatus in pond, cellar for storing things, the through hole on cell body or pressing plate makes poor unstrained spirits be communicated with culture dish, to carry out pond, cellar for storing things microorganism in situ culture experiment.
Particularly, described pressing plate is fixedly installed on the cell body of groove top by screw.
Further, the two ends of described cell body arrange suitable syndeton respectively.In order to different depths carries out Culture in situ test in pond, cellar for storing things, above-mentioned at least two pond, joint cellar for storing things microorganism in situ culture apparatuses are connected by syndeton, form longer pond, cellar for storing things microorganism in situ culture apparatus.
Further, described through hole is only arranged on pressing plate, cell body is arranged at least two grooves, and the independent of one another and length direction along cell body of each groove is arranged.
Further, pond, described cellar for storing things microorganism in situ culture apparatus also comprises plug, and one end of described plug is circular cone or pyramid-shaped, and the other end of plug arranges the syndeton suitable with any one end of described cell body.Plug is connected to one end of cell body, can more easily pond, cellar for storing things microorganism in situ culture apparatus be inserted in the poor unstrained spirits in pond, cellar for storing things by plug.
Further, pond, described cellar for storing things microorganism in situ culture apparatus also comprises handle, and the middle part of handle arranges the syndeton suitable with any one end of described cell body.By handle, be convenient to pond, cellar for storing things microorganism in situ culture apparatus to insert in the poor unstrained spirits in pond, cellar for storing things, but also be convenient to after cultivation terminates, effortlessly pond, cellar for storing things microorganism in situ culture apparatus is extracted from the poor unstrained spirits in pond, cellar for storing things.
Particularly, described syndeton is suitable female adapter and male connection.
Further, pond, described cellar for storing things microorganism in situ culture apparatus is made up of stainless steel.
The present invention also provides a kind of and carries out storing by any one cellar for storing things pond microorganism in situ culture apparatus above-mentioned the method that pond microorganism in situ cultivates: be first fixedly installed in groove by culture dish by pressing plate, then pond, cellar for storing things microorganism in situ culture apparatus is put into Jiao Chi carries out Culture in situ.
Particularly, the height of described culture dish is consistent with the degree of depth of groove, and the sidewall of groove and bottom are all without through hole, and the bottom of culture dish arranges at least one hole and lays millipore filtration in the bottom of culture dish; Culture dish is positioned in groove, and the bottom of culture dish is positioned at pressing plate side, then is fixedly installed in groove by culture dish by pressing plate.
The invention has the beneficial effects as follows: cellar for storing things pond microorganism in situ culture apparatus and method can under the in situ environment of Jiao Chi culturing micro-organisms, independently can determine incubation time and cultivate position, structure is simple, easy to operate.Be conducive to further investigation white wine microorganism, comprehensively the diversity of pond, reflection cellar for storing things microorganism, and set up Microbial resources storehouse.
Accompanying drawing explanation
Fig. 1 is the structural representation that the present invention stores pond microorganism in situ culture apparatus;
Fig. 2 is the side-view of pond, cellar for storing things shown in Fig. 1 microorganism in situ culture apparatus;
Fig. 3 is the structural representation that the present invention stores another embodiment of pond microorganism in situ culture apparatus.
Component, position and numbering in figure: cell body 1, groove 2, pressing plate 3, screw 4, plug 5, handle 6.
Embodiment
Below in conjunction with accompanying drawing, the invention will be further described.
As depicted in figs. 1 and 2, the present invention stores pond microorganism in situ culture apparatus, comprises cell body 1 and pressing plate 3, described cell body 1 is at least arranged a groove 2, as diagram arranges three grooves 2; The top of groove 2 arranges pressing plate 3, and pressing plate 3 is removably installed on the cell body 1 at the top of groove 2, and particularly, pressing plate 3 is fixedly installed on the cell body 1 at groove 2 top by screw 4; Culture dish is positioned in groove 2, then pressing plate 3 is fixedly mounted by screw 4, culture dish is fixed in groove 2.In order to make culture dish keep being communicated with the external world, cell body 1 and/or pressing plate 3 at least arrange a through hole, and groove 2 is in communication with the outside by through hole.Arrange through hole as illustrated on pressing plate 3, the sidewall of groove 2 and bottom are all without through hole.Through hole on pressing plate 3 does not affect culture dish and is fixed in groove 2, and retain pressing plate 3 through hole has larger diameter simultaneously, is convenient to culture dish inside and fully carries out material, information interchange with outside atmosphere.
Cell body 1 is arranged three cylindrical grooves, 2, three grooves 2 and be arranged at the same side of cell body 1 respectively and the length direction along cell body 1 is arranged, the size dimension of three grooves 2 is consistent.Arrange pressing plate 3 above groove 2, pressing plate 3 can be as a whole, also can be an a groove 2 corresponding pressing plate 3 respectively.Pressing plate 3 arranges through hole, and through hole is circular hole, and the diameter of circular hole is less than the diameter of groove 2 cross section.In order to ensure that pressing plate 3 can culture dish in fastening grooves 2, be convenient to take off separately each culture dish, each groove 2 arranges separately pressing plate 3 simultaneously, and the surrounding of pressing plate 3 evenly arranges four screws 4.For avoiding pressing plate 3 to hinder culture dish inside and being communicated with between outside, pressing plate 3 is arranged a circular hole.
Pond, above-mentioned cellar for storing things microorganism in situ culture apparatus is directly positioned in the poor unstrained spirits in pond, cellar for storing things, can Culture in situ be carried out.
Cell body 1 two ends are shaft-like, and middle part is flat-shaped, and wherein the middle part of cell body 1 is for arranging the region of groove 2.For the ease of carrying out Culture in situ test at the different depths of Jiao Chi, different positions, the two ends of cell body 1 arrange suitable syndeton respectively.At least two joint cell bodies 1 are joined end to end, forms longer pond, cellar for storing things microorganism in situ culture apparatus.Particularly, the syndeton at cell body 1 two ends is respectively suitable female adapter and male connection.
As shown in Figure 3, for the ease of cell body 1 being inserted in the poor unstrained spirits in pond, cellar for storing things, pond, cellar for storing things microorganism in situ culture apparatus also comprises plug 5, and one end of plug 5 is circular cone or pyramid-shaped, and the other end of plug 5 is the female adapter suitable with cell body 1 termination or male connection.Pond, cellar for storing things microorganism in situ culture apparatus also comprises handle 6, and the middle part of handle 6 arranges the female adapter suitable with the termination of cell body 1 or male connection, and the joint of handle 6 is not identical with the joint of plug 5.By handle 6, be convenient to pond, cellar for storing things microorganism in situ culture apparatus to insert in the poor unstrained spirits in pond, cellar for storing things, but also be convenient to after cultivation terminates, effortlessly pond, cellar for storing things microorganism in situ culture apparatus is extracted from the poor unstrained spirits in pond, cellar for storing things.Three sections of cell bodies 1 are joined end to end respectively, reconnect plug 5 and handle 6, just can carry out the Culture in situ operation of microorganism in arbitrary levels, arbitrarily the fermentation period of pond, cellar for storing things upper, middle and lower-ranking grain unstrained spirits easily, likely greatly improve the Culturability of microorganism.
Pond, whole cellar for storing things microorganism in situ culture apparatus is made by stainless steel, and namely cell body 1, pressing plate 3, screw 4, plug 5, handle 6 are made by stainless steel.
The present invention also provides a kind of method of carrying out storing pond microorganism in situ cultivation by pond, above-mentioned cellar for storing things microorganism in situ culture apparatus, first by pressing plate 3, culture dish is fixedly installed in groove 2, more poor unstrained spirits pond, cellar for storing things microorganism in situ culture apparatus being put into Jiao Chi carries out Culture in situ.Wherein, the height of culture dish is consistent with the degree of depth of groove 2, and the total height namely after culture dish covers adaptive culture dish lid is consistent with the degree of depth of groove 2, to ensure effectively to fix by pressing plate 3 pairs of culture dish; The sidewall of groove 2 and bottom are all without through hole, and the bottom of culture dish arranges at least one hole, such as, arrange 5 to 6 circular holes at the bottom even of culture dish, and lay millipore filtration in the bottom of culture dish; Culture dish is positioned in groove 2, and the bottom of culture dish is positioned at the side at pressing plate 3 place, then is fixedly installed in groove 2 by culture dish by pressing plate 3.
Cellar for storing things pond microorganism in situ culture apparatus and method are suitable for the Culture in situ test of aromatic Chinese spirit fermentation pit microorganism, culturing micro-organisms in pond spontaneous fermentation process can stored, and independently can determine incubation time and cultivate position, structure is simple, easy to operate.
Claims (10)
1. store pond microorganism in situ culture apparatus, it is characterized in that: comprise cell body (1) and pressing plate (3), described cell body (1) is at least arranged a groove (2), the top of groove (2) arranges described pressing plate (3), described pressing plate (3) is removably installed on the cell body (1) at the top of groove (2), described cell body (1) and/or pressing plate (3) at least arrange a through hole, and groove (2) is in communication with the outside by described through hole.
2. pond, cellar for storing things as claimed in claim 1 microorganism in situ culture apparatus, is characterized in that: described pressing plate (3) is fixedly installed on the cell body (1) at groove (2) top by screw (4).
3. pond, cellar for storing things as claimed in claim 1 microorganism in situ culture apparatus, is characterized in that: the two ends of described cell body (1) arrange suitable syndeton respectively.
4. pond, cellar for storing things as claimed in claim 1 microorganism in situ culture apparatus, it is characterized in that: described through hole is only arranged on pressing plate (3), cell body (1) is upper arranges at least two grooves (2), and the independent of one another and length direction along cell body (1) of each groove (2) is arranged.
5. pond, cellar for storing things as claimed in claim 1 microorganism in situ culture apparatus, it is characterized in that: pond, described cellar for storing things microorganism in situ culture apparatus also comprises plug (5), one end of described plug (5) is circular cone or pyramid-shaped, and the other end of plug (5) is arranged and described cell body (1) syndeton that one end is suitable arbitrarily.
6. pond, cellar for storing things as claimed in claim 1 microorganism in situ culture apparatus, it is characterized in that: pond, described cellar for storing things microorganism in situ culture apparatus also comprises handle (6), the middle part of handle (6) is arranged and described cell body (1) syndeton that one end is suitable arbitrarily.
7. pond, the cellar for storing things microorganism in situ culture apparatus as described in the arbitrary claim of claim 3 or 5 or 6, is characterized in that: described syndeton is suitable female adapter and male connection.
8. pond, cellar for storing things as claimed in claim 1 microorganism in situ culture apparatus, is characterized in that: pond, described cellar for storing things microorganism in situ culture apparatus is made up of stainless steel.
9. store the method that pond microorganism in situ is cultivated, it is characterized in that: carry out pond, cellar for storing things microorganism in situ by pond, the cellar for storing things microorganism in situ culture apparatus described in the arbitrary claim of the claims 1 to 8 and cultivate: first by pressing plate (3), culture dish is fixedly installed in groove (2), then put into Jiao Chi carry out Culture in situ by storing pond microorganism in situ culture apparatus.
10. the method for pond, cellar for storing things as claimed in claim 9 microorganism in situ cultivation, it is characterized in that: the height of described culture dish is consistent with the degree of depth of groove (2), the sidewall of groove (2) and bottom are all without through hole, and the bottom of culture dish arranges at least one hole and lays millipore filtration in the bottom of culture dish; Culture dish is positioned in groove (2), and the bottom of culture dish is positioned at pressing plate side, then is fixedly installed in groove (2) by culture dish by pressing plate (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510931179.7A CN105368700B (en) | 2015-12-15 | 2015-12-15 | Pit microorganism in situ culture apparatus and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510931179.7A CN105368700B (en) | 2015-12-15 | 2015-12-15 | Pit microorganism in situ culture apparatus and method |
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| Publication Number | Publication Date |
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| CN105368700A true CN105368700A (en) | 2016-03-02 |
| CN105368700B CN105368700B (en) | 2017-12-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510931179.7A Active CN105368700B (en) | 2015-12-15 | 2015-12-15 | Pit microorganism in situ culture apparatus and method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5417576A (en) * | 1993-11-12 | 1995-05-23 | Iowa Methodist Medical Center | Means and method for microbiological growth and in situ observation with microscopes |
| CN104789449A (en) * | 2015-04-27 | 2015-07-22 | 国家海洋局第一海洋研究所 | In-situ culturing device for deep-sea microbes |
| CN104807672A (en) * | 2015-05-08 | 2015-07-29 | 泸州品创科技有限公司 | Multi-point vinasse sampler and sampling method |
-
2015
- 2015-12-15 CN CN201510931179.7A patent/CN105368700B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5417576A (en) * | 1993-11-12 | 1995-05-23 | Iowa Methodist Medical Center | Means and method for microbiological growth and in situ observation with microscopes |
| CN104789449A (en) * | 2015-04-27 | 2015-07-22 | 国家海洋局第一海洋研究所 | In-situ culturing device for deep-sea microbes |
| CN104807672A (en) * | 2015-05-08 | 2015-07-29 | 泸州品创科技有限公司 | Multi-point vinasse sampler and sampling method |
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| CN105368700B (en) | 2017-12-08 |
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