CN210163444U

CN210163444U - Siphon pipe feed supplement shaking table

Info

Publication number: CN210163444U
Application number: CN201822174039.1U
Authority: CN
Inventors: 刘志强; 沈水兴; 沈昊; 刘政
Original assignee: SHANGHAI ZHICHENG ANAIYTICAL INSTRUMENT MANUFACTURING Co Ltd
Current assignee: SHANGHAI ZHICHENG ANAIYTICAL INSTRUMENT MANUFACTURING Co Ltd
Priority date: 2018-12-24
Filing date: 2018-12-24
Publication date: 2020-03-20
Anticipated expiration: 2028-12-24

Abstract

A siphon tube material-supplementing shaking table is characterized by that it uses material-supplementing shaking table as basis, uses functional siphon tube solid culture connector to make several shaking bottles be series-connected, and uses the first culture bottle to add target strain, and utilizes the siphon tube to make solid-liquid alternative fermentation until the last shaking bottle is finished so as to obtain the invented quick multiple culture. The utility model provides a new technical means for utilizing the feeding table to carry out micro-biological strain domestication, separation and purification, which is suitable for being widely applied to the fields of food, medicine, feed, biological products and the like.

Description

Siphon pipe feed supplement shaking table

Technical Field

The utility model relates to a technical method and shaking table equipment manufacturing field of biological research especially relate to a siphon pipe feed supplement shaking table.

Background

The domestication, separation and purification of the strains are important technical means for the research of microbial culture, and the three are independent and mutually related. Domestication is the first one, and is based on Darwinian biological evolution theory 'environmental selection and fittest survival', and is divided into three different ecological niche adjustment processes, wherein the first ecological niche adjustment is to restore strains by liquid culture in a room (bacterial sludge) under the original nutrition state, then the strains can be identified by naked eyes through solid culture, so that the adapted strains under the new environment are restored, and the unsuitable strains are eliminated. The second ecological niche adjustment is to change the culture medium environment according to needs, adapt to survival and separate and eliminate more strains; the third niche adjustment is the selection of individual strains by changing the culture environment again and the stimulation of their working capacity for production. Each process is that solid culture and liquid shaking table fermentation are alternately carried out and repeated for many times.

Therefore, the shaking table is an important tool for researching microorganisms by utilizing the shaking fermentation of the triangular shaking bottle and has a long history and wide application. The feeding table is used for timely feeding nutrition into the triangular shake flask and adjusting the pH of fermentation liquor in the flask to improve the fermentation environment and prolong the fermentation time and increase fermentation products; the feeding endows the traditional shaking table with new functions.

SUMMERY OF THE UTILITY MODEL

The utility model provides a siphon pipe feed table is add functional siphon pipe solid culture connector on feed table basis, sets up the solid medium in the pipe, makes present solid-liquid cultivate respectively in turn and fuses once accomplishing on the shaking table, and the siphon pipe is the major technological improvement to feed table application.

The utility model discloses a following scheme realizes:

a siphon pipe feeding shaking table comprises a feeding shaking table and a plurality of functional siphon pipe solid culture connectors, wherein the feeding shaking table comprises a plurality of shaking bottles and feeding bottles, a pH meter detection electrode and a feeding needle head are inserted into each shaking bottle, the feeding needle heads are connected to the feeding bottles through pipelines and precise micro pumps, each functional siphon pipe solid culture connector comprises a siphon pipe, needle heads are arranged at two ends of each siphon pipe, are respectively inserted into two adjacent shaking bottles and are not in contact with the liquid level in the shaking bottles, an adsorption carrier and a solid culture medium are arranged in each siphon pipe, and all the shaking bottles are sequentially connected in series through the functional siphon pipe solid culture connectors.

In the above technical scheme, the siphon catheter can be a silicone tube, a medical plastic tube, a stainless steel tube or a glass tube.

The inner diameter of the siphon guide pipe is usually 0.25-5.0 mm, and the length of the siphon guide pipe is usually 30-300 mm.

The two ends of the siphon catheter are needles of 8-20 gauge, and the length of the needle handle is 25-150 mm.

The adsorption carrier material in the siphon conduit comprises cotton thread, absorbent cotton, nano artificial fiber, absorbent gel and agar.

The feeding table preferably adopts a ZWYB-292 feeding table manufactured by Shanghai Zhicheng analytical instrument manufacturing company Limited.

The utility model discloses an useful part lies in:

1) the strain domestication operation process is simplified, and the prior strain domestication is still finished by liquid culture after a target strain is obtained by plate solid culture and selection and is repeatedly and alternately finished for multiple times. If a certain strain of bacteria needs 24 hours for acclimatization and fermentation each time, the acclimatization and culture are carried out for 6 times, the strain is transferred to solid culture after liquid fermentation and culture, then the strain is selected to enter the next liquid culture, and the repeated and complete completion time needs at least two weeks or even longer; the siphon catheter connector technology is adopted, firstly, the type of the catheter material is selected, the diameter and the length of the catheter with the adsorption carrier material in the catheter and the type of a catheter needle are determined, each section of catheter is placed into the set solid culture medium, domestication and culture are carried out for 6 times, only 1 time is needed for strain transfer, and only 3-5 days are needed for apparent acclimation.

2) The method can be used for standardizing operation and saving the experiment cost, preparing the solid culture medium in the siphon guide pipe, strictly operating at one time, producing in batches to prepare the standard biochemical kit, sealing and storing, repeatedly operating, reducing the artificial experiment error and saving a large amount of experimental equipment.

3) A functional siphon conduit material supplementing shaking table is characterized in that a solid culture medium is arranged in a conduit, so that the prior solid-liquid alternative culture and fusion are respectively completed on the shaking table at one time, and a siphon conduit is a great technical improvement on the application of the material supplementing shaking table, brings a new market development space for the development and application of products of the shaking table, and is suitable for being widely applied to the fields of food, medicine, feed, biological products and the like.

To sum up, the utility model discloses an innovation part lies in:

1) through a siphon conduit solid culture connector, strains are domesticated, separated and purified alternately and respectively cultured, so that the strains are simply integrated on a shaking table, and are inoculated once and continuously fermented for multiple times until a high-quality product is obtained;

2) the Darwinian biological evolution theory is used as a guide basis to 'survive by selecting a suitable person in the environment', a solid culture medium in a siphon guide pipe is strictly arranged and used as a relevant key for the selection and adaptation of the guide pipe length, the guide pipe material, the needle diameter size, the in-pipe culture medium carrier preparation and the like of a connector to microorganisms such as domesticated and cultured yeasts, bacteria, yeasts, actinomycetes, viruses and the like, and the application field is very wide.

3) The functional conduit connector is used as a new strain breeding technology, is made into a standardized preparation box which can store massive biotechnological information and is convenient for universal exchange of strain resources, so that good strain domestication, separation and purification not only depend on strains, but also which feeding table siphon conduit connector is adopted, the application effect of the analysis table is greatly improved, and the technical blank that the analysis table is used as a product development application table for a long time is filled.

Drawings

FIG. 1 is a schematic view of the structure of a feeder-shaker;

FIG. 2 is a schematic view of the siphon catheter feeding table of the present invention;

FIG. 3 is a schematic diagram of the connection of siphon pipes between the flasks;

FIG. 4 is a schematic structural diagram of a functional siphon tube solid culture connector, wherein, a 16-1 needle, a 16-2 siphon tube carrier, a 16-3 silicone tube or a transparent plastic tube, a 16-4 stainless steel tube and a 16-5 glass tube.

Detailed Description

The present invention will be further explained with reference to the drawings and examples.

In the example, on the basis of ZWYB-292 type feeding table of Shanghai Zhicheng analytical instrument manufacturing Limited company, a functional siphon pipe solid culture connector is additionally arranged to ensure that a plurality of shake flasks are connected in series for fermentation, a target strain is added from a first culture flask, the siphon pipe is communicated to form solid-liquid alternate fermentation until the last shake flask is finished, and purposeful rapid multiple culture is further obtained.

The ZWYB-292 type feeding table is a limit 6-bottle fermentation, double-display large-screen touch control panel and closed-loop micro-pulse accurate feeding table, and keeps the biological culture environment in an optimal control state; PH control, nutrition supplement, temperature and rotating speed are continuously adjustable; wherein the PH adjustment and the nutrition supplement are respectively controlled by a micro electromagnetic pump, and the precision reaches micro-millilitre level; the adopted double-display large-screen touch controller realizes man-machine operation, is visual and convenient, displays six PH values and growth corresponding curves and trace acid regulating process curves of 6 shake flask reactors in real time, and has advanced functions of online observation, recording, storage, comparative analysis, remote data output and the like in the fermentation process (figure 1). When in use, the bottom plate 1 is taken out, a fermentation bottle (namely a shake flask) 2 is assembled, the triangular flask is numbered clockwise as 2-I-2-VI, a proper amount of prepared nutrient solution is pre-added in the flask, a cotton plug is covered on the flask opening 2-1, a rubber cap is covered on the flask opening 2-2, a PH meter detection electrode 4 and an alkaline solution replenishing pipe needle 5 are inserted after being tightly sealed, the other end of the alkaline solution replenishing pipe is connected with the output end 4-I-4-VI of the alkaline solution replenishing precision micro-pump 8, and one corresponding input end pipeline (1 for 6) is connected with an alkaline solution replenishing flask 7; the bottle mouth 2-3 is covered with a rubber cap, the needle heads 3 of the nutrient solution supplementing pipes are respectively inserted into the rubber cap, the other end of each nutrient solution supplementing pipe is connected with the output ends 3-I-3-VI of the nutrient solution supplementing precise micro pumps 9, and one corresponding input end pipeline (1 supply 6) is connected with the nutrient solution supplementing bottle 6; after the connection is completed, the whole bottom plate 1 is sterilized, after the whole bottom plate is cooled, strains are respectively added into shake flasks 2-I-2-VI under the aseptic condition, then the bottom plate 1 is arranged on a shaking table, and the shake flasks 2-I-2-VI are simultaneously subjected to shaking culture.

The siphon pipe feeding shaking table is characterized in that siphon pipe solid culture connectors (shown in figure 2) to (15) are added on the basis of a ZWYB-292 type feeding shaking table (shown in figure 1), the connection method is that one end of a siphon pipe needle is inserted into a rubber cap at the bottle mouth 2-3 of a shake flask 2-I, the other end of the siphon pipe needle is inserted into a rubber cap at the bottle mouth 2-3 of a shake flask 2-II to form a siphon pipe I, and the siphon pipe I and the siphon pipe V are sequentially connected to form siphon pipes I to V (shown in figure 3). The siphon guide pipe is made of the following materials: silicone tube, medical plastic tube or special stainless steel tube, glass tube, etc.; the inner diameter is 0.25-5.0 mm, the length is 30-300 mm, and the inner diameter can be set according to requirements; the needle heads used at the two ends of the catheter are 8-20 gauge needle heads, and the length of the needle handle is 25-150 mm; the carrier material in the siphon catheter comprises cotton thread, absorbent cotton, nano artificial fiber, or absorbent gel, agar gel and the like; the nutrient solution absorbed by the carrier material in the siphon catheter is consistent with the nutrition in the culture bottle 2 or is prepared according to the requirement; when in use, only strains are added into the shake flask 2-I, every two needles of the siphon pipe solid culture communicating vessel are inserted into the fermentation flask openings 2-3-I-2-3-VI to be connected in series, so that the alternation of shake flask liquid fermentation and siphon pipe solid fermentation is formed, and finally, the product is obtained in the shake flask 2-VI.

Claims

1. The siphon catheter material-supplementing shaking table is characterized by comprising a material-supplementing shaking table and a plurality of functional siphon catheter solid culture connectors, wherein the material-supplementing shaking table comprises a plurality of shaking bottles and material-supplementing bottles, a pH meter detection electrode and a material-supplementing needle head are inserted into each shaking bottle, the material-supplementing needle heads are connected to the material-supplementing bottles through precise micro pumps through pipelines, the functional siphon catheter solid culture connectors comprise siphon catheters, needle heads are arranged at two ends of each siphon catheter, are respectively inserted into two adjacent shaking bottles and are not in contact with the liquid level in the shaking bottles, adsorption carriers and solid culture mediums are arranged in the siphon catheters, and all the shaking bottles are sequentially connected in series through the functional siphon catheter solid culture connectors.

2. The siphon catheter feeding and shaking table according to claim 1, wherein the siphon catheter is silicone tube, medical plastic tube, stainless steel tube or glass tube.

3. The siphon tube feeder according to claim 1, wherein the siphon tube has an inner diameter of 0.25 to 5.0mm and a length of 30 to 300 mm.

4. The siphon tube material feeding table according to claim 1, wherein the needles at both ends of the siphon tube are 8-20 gauge needles, and the length of the needle handle is 25-150 mm.

5. The siphon catheter feeding shaker of claim 1, wherein the feeding shaker is a ZWYB-292 feeding shaker from Shanghai Zhicheng analytical instruments manufacturing Limited.