CN105362591A - Pharmaceutical composition and preparation method, preparation and application thereof - Google Patents
Pharmaceutical composition and preparation method, preparation and application thereof Download PDFInfo
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- CN105362591A CN105362591A CN201510912778.4A CN201510912778A CN105362591A CN 105362591 A CN105362591 A CN 105362591A CN 201510912778 A CN201510912778 A CN 201510912778A CN 105362591 A CN105362591 A CN 105362591A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/894—Dioscoreaceae (Yam family)
- A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1664—Compounds of unknown constitution, e.g. material from plants or animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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Abstract
The invention discloses a pharmaceutical composition and a preparation method, a preparation and application thereof. The pharmaceutical composition comprises radix angelicae, rhizoma dioscoreae and poria cocos in a weight ratio of 1-3:1-3:1-3. The preparation method includes the steps of pretreatment, coarse pulverization, superfine pulverization and blending. The preparation is prepared into powder, tablets, granules, capsules, dripping pills, honeyed pills or external application by adding pharmaceutically acceptable minor ingredients into the pharmaceutical composition. The pharmaceutical composition is applied to preparation of whitening and beautifying drugs or cosmetics. The pharmaceutical composition is prepared by blending active pharmaceutical ingredients according to a precise composition, achieves whitening and beautifying effects by performing compatibility according to physical and chemical properties and active constituents of the active pharmaceutical ingredients, and has the advantages of high bioavailability, high active constituent dissolution speed, good pharmaceutical absorption effect and little side effect.
Description
Technical field
The invention belongs to Chinese drug preparation technique field, be specifically related to a kind of pharmaceutical composition and preparation method thereof, preparation and application.
Background technology
Skin refers to that body surface wraps in the tissue outside muscle, is the maximum organ of human body, mainly carries protection health, perspire, feels cold and hot and the function such as pressure.Skin covers whole body, and it to make in body various tissue and organ from the invasion and attack of physical property, mechanicalness, chemical and pathogenic microorganism.The skin of people and higher mammal is made up of epidermis, corium (mesoderm), subcutaneous tissue three layers.5% ~ 15% of skin (Skin) gross weight percentage of liveweight, the gross area is 1.5 ~ 2 square metres, and thickness is different because of people or position, is 0.5 millimeter ~ 4 millimeters.Skin covers whole body, and it to make in body various tissue and organ from the invasion and attack of physical property, mechanicalness, chemical and pathogenic microorganism.Skin has the barrier action of two aspects: prevent moisture content in body, electrolyte, other materials loss on the one hand; Stop the intrusion of extraneous harmful substance on the other hand.Skin remains the stable of human internal environment, and skin also participates in the metabolic process of human body simultaneously.Skin has several color (white, yellow, red, brown, black etc.), mainly different because ethnic group, age and position are different.Skin is made up of epidermis, corium and subcutaneous tissue, and containing accessory organ's (sweat gland, sebaceous gland, fingernail, toenail) and blood vessel, lymphatic vessel, N&M etc.Skin is the maximum organ of people's bulk area.The skin developed area of an adult is at 2 square meter, and weight is about 1/20 of human body weight.The thickest skin is in vola portion, and thickness reaches 4 millimeters, and the skin on eyelid is the thinnest, only less than 1 millimeter.Face is people's shop front, and divide to the primary impression of people, face account for important proportion, so facial skin maintenance is the key in key.Along with the development of society, people are also more and more higher for external requirement, and the associated class product of existing market can not meet the demand of people gradually, therefore, develop a kind of product that can solve the problem and are very important.
Summary of the invention
The first object of the present invention is to provide a kind of pharmaceutical composition; Second object is the preparation method providing described pharmaceutical composition; 3rd object is the preparation providing described pharmaceutical composition; 4th object is the application providing described pharmaceutical composition.
The first object of the present invention is achieved in that described pharmaceutical composition comprises the Radix Angelicae Dahuricae, Rhizoma Dioscoreae and Poria, and weight ratio is 1 ~ 3:1 ~ 3:1 ~ 3.
The second object of the present invention is achieved in that and comprises pretreatment, coarse pulverization, micronizing and mixture operation, specifically comprises:
A, pretreatment: the foreign material in each raw material are rejected, through selected, cleaning, dry in the sun, to be dried to moisture content≤3% for subsequent use;
B, coarse pulverization: the raw material Radix Angelicae Dahuricae, Rhizoma Dioscoreae, Poria and Radix Salviae Miltiorrhizae are pulverized with Roughpulverizer respectively, crosses 80 ~ 140 mesh sieves for subsequent use;
C, micronizing: the micropowders each raw material after coarse pulverization being obtained respectively each raw material through micronizing;
D, mixture: each raw material micropowders mixture is evenly obtained object by formulation ratio.
The third object of the present invention is achieved in that in described pharmaceutical composition that adding pharmaceutically acceptable adjuvant makes powder, tablet, granule, capsule, drop pill, honey pill agent or externally dressing.
The fourth object of the present invention is achieved in that described pharmaceutical composition is preparing the application in beauty face-whitening-nourishing medicine or cosmetics.
When pharmaceutical composition of the present invention takes orally and uses, in prescription, Poria has significant competitive inhibition to tryrosinase, and tryrosinase is the key enzyme that melanin is produced, and reduces melanin produce by restraint of tyrosinase activity; The Radix Angelicae Dahuricae has sterilization, antiinflammatory action, can either detumescence and apocenosis, eliminates facial acne, can also local blood circulation be improved, eliminate pigment excess accumulation in the tissue, promote skin cell metabolism, treatment acne, acne rosacea, freckle and chloasma faciei, thus reach the effect of beauty treatment; Rhizoma Dioscoreae can be promoted the production of body fluid and be moisturized, nourishes rough skin, and skin is moistened; Radix Salviae Miltiorrhizae has promoting blood flow to regulate menstruation, antiinflammatory action.
When pharmaceutical composition external application of the present invention uses, in prescription, Poria has significant competitive inhibition to tryrosinase, and tryrosinase is the key enzyme that melanin is produced, and reduces melanin produce by restraint of tyrosinase activity; The Radix Angelicae Dahuricae has sterilization, antiinflammatory action, can either detumescence and apocenosis, eliminates facial acne, can also local blood circulation be improved, eliminate pigment excess accumulation in the tissue, promote skin cell metabolism, treatment acne, acne rosacea, freckle and chloasma faciei, thus reach the effect of beauty treatment; Rhizoma Dioscoreae can be promoted the production of body fluid and be moisturized, nourishes rough skin, and skin is moistened.
Pharmaceutical composition of the present invention is made according to the prescription mixture of accurately setting, carry out according to former medicine reason, chemical property and contained active component the efficacy effect that compatibility reaches looks improving and the skin nourishing, and have that bioavailability is high, active component stripping is fast, drug absorption is effective and the feature that side effect is little.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated, but limited the present invention never in any form, and any conversion done based on training centre of the present invention or replacement, all belong to protection scope of the present invention.
Pharmaceutical composition of the present invention comprises the Radix Angelicae Dahuricae, Rhizoma Dioscoreae and Poria, and weight ratio is 1 ~ 3:1 ~ 3:1 ~ 3.
Described pharmaceutical composition also comprises Radix Salviae Miltiorrhizae, and the weight ratio of the Radix Angelicae Dahuricae in described pharmaceutical composition, Rhizoma Dioscoreae, Poria and Radix Salviae Miltiorrhizae is 1 ~ 3:1 ~ 3:1 ~ 3:1 ~ 3.
The weight ratio of the Radix Angelicae Dahuricae in described pharmaceutical composition, Rhizoma Dioscoreae, Poria and Radix Salviae Miltiorrhizae is 2:2:2:2.
The preparation method of pharmaceutical composition of the present invention, comprises pretreatment, coarse pulverization, micronizing and mixture operation, specifically comprises:
A, pretreatment: the foreign material in each raw material are rejected, through selected, cleaning, dry in the sun, to be dried to moisture content≤3% for subsequent use;
B, coarse pulverization: the raw material Radix Angelicae Dahuricae, Rhizoma Dioscoreae, Poria and Radix Salviae Miltiorrhizae are pulverized with Roughpulverizer respectively, crosses 80 ~ 140 mesh sieves for subsequent use;
C, micronizing: the micropowders each raw material after coarse pulverization being obtained respectively each raw material through micronizing;
D, mixture: each raw material micropowders mixture is evenly obtained object by formulation ratio.
The particle diameter of described micropowders is 6 ~ 12 μm.
The preparation of pharmaceutical composition of the present invention is add pharmaceutically acceptable adjuvant in described pharmaceutical composition to make powder, tablet, granule, capsule, drop pill, honey pill agent or externally dressing.
The described pharmaceutical composition that is applied as of pharmaceutical composition of the present invention is preparing the application in beauty face-whitening-nourishing medicine or cosmetics.
Described pharmaceutical composition instructions of taking is for take with boiled water or Mel, and three times on the one, taking dose is 2g/ time.
The Chinese liquor external application 15 ~ 25min of Mel, milk or 40 ~ 60 degree is added in described pharmaceutical composition.
Pharmaceutical composition of the present invention is for oral administration+external application time effect more remarkable.
With specific embodiment, the present invention will be further described below:
Embodiment 1
---the preparation method of described pharmaceutical composition
A, pretreatment: get Radix Angelicae Dahuricae 50kg, Radix Salviae Miltiorrhizae 50kg, Rhizoma Dioscoreae 50kg, be placed in the silt etc. that SUS304 rustless steel XSG-600 type circulating water washer utilizes the rolling friction removing medical material of Water spray and the cleaning of general water and medical material respectively, drum speed is 12rpm, after medical material foreign material are wherein rejected, choose, cut into slices again, the Radix Angelicae Dahuricae (sheet) after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 65 DEG C, Radix Salviae Miltiorrhizae after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 70 DEG C, Rhizoma Dioscoreae after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 75 DEG C, then by the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae is placed in RXH type hot air circular drying machine respectively, be dried to moisture content≤3% for subsequent use.
Get Poria 50kg, carry out artificial rinsing with flowing water, the Poria through rinsing cleaning cleans, and is rejected by foreign material wherein, after Poria is placed in steam stove and steams to the saturating heart, peel, dice, the Poria after dicing is placed in baking oven sterilizing, sterilization time is set as 9min, sterilising temp is 70 DEG C, then Poria is placed in RXH type hot air circular drying machine, is dried to moisture content≤3% for subsequent use.
B, coarse pulverization: the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Poria, Radix Salviae Miltiorrhizae Circoplex classified grinding machine are pulverized, it is for subsequent use that Radix Angelicae Dahuricae coarse powder crosses 80 mesh sieves, it is for subsequent use that Radix Salviae Miltiorrhizae coarse powder crosses 80 mesh sieves, it is for subsequent use that coarse yam powder crosses 80 mesh sieves, it is for subsequent use that Poria coarse powder crosses 80 mesh sieves, microorganism detection is carried out in the Radix Angelicae Dahuricae, Rhizoma Dioscoreae, Poria, the sampling of Radix Salviae Miltiorrhizae coarse powder, and the Radix Angelicae Dahuricae after qualified, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae, Poria coarse powder carry out micronizing.
C, micronizing: the Radix Angelicae Dahuricae after Micro biological Tests is qualified, Radix Salviae Miltiorrhizae coarse powder WFM100 type ultra micro vibromill are pulverized, working condition is set as: grinding media is bar-shaped, grinding media diameter is 0.3mm, grinding media pack completeness is 50%, Radix Angelicae Dahuricae inventory is 10kg, grinding time is 30min, Radix Salviae Miltiorrhizae inventory is 9kg, grinding time is 30min, Poria inventory is 10kg, and grinding time is 6h, and Rhizoma Dioscoreae dosage is 12kg, grinding time is 30min, and in the Radix Angelicae Dahuricae after pulverizing, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae, Poria micropowders, particle diameter is the fine powder ratio of 6 ~ 12um is 92%.After the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae, Poria micropowders are crossed 200 mesh sieves, the micropowders having crossed sieve is placed in rustless steel CBS series drawer type permanent-magnetic iron expeller and removes individual metal class material.
D, mixture: by Radix Angelicae Dahuricae micropowders 50 parts by weight, Rhizoma Dioscoreae micropowders 50 part, part Poria micropowders 50 parts, Radix Salviae Miltiorrhizae micropowders 50 parts mixing, be placed in EYH-1000A type two-dimensional motion mixer mix and blend, rotation number is 8rpm, shake number is 6rpm, mixing time is 30min, after mixture is even, in pharmaceutical composition, particle diameter is the fine powder ratio of 4 ~ 14um is 92%.
Embodiment 2
---the preparation method of described pharmaceutical composition
A, pretreatment: get Radix Angelicae Dahuricae 50kg, Radix Salviae Miltiorrhizae 150kg, Rhizoma Dioscoreae 100kg, be placed in the silt etc. that SUS304 rustless steel XSG-600 type circulating water washer utilizes the rolling friction removing medical material of Water spray and the cleaning of general water and medical material respectively, drum speed is 12rpm, after medical material foreign material are wherein rejected, choose, cut into slices again, the Radix Angelicae Dahuricae (sheet) after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 65 DEG C, Radix Salviae Miltiorrhizae after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 70 DEG C, Rhizoma Dioscoreae after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 75 DEG C, then by the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae is placed in RXH type hot air circular drying machine respectively, be dried to moisture content≤3% for subsequent use.
Get Poria 100kg, carry out artificial rinsing with flowing water, the Poria through rinsing cleaning cleans, and is rejected by foreign material wherein, after Poria is placed in steam stove and steams to the saturating heart, peel, dice, the Poria after dicing is placed in baking oven sterilizing, sterilization time is set as 9min, sterilising temp is 70 DEG C, then Poria is placed in RXH type hot air circular drying machine, is dried to moisture content≤3% for subsequent use.
B, coarse pulverization: the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Poria, Radix Salviae Miltiorrhizae Circoplex classified grinding machine are pulverized, it is for subsequent use that Radix Angelicae Dahuricae coarse powder crosses 140 mesh sieves, it is for subsequent use that Radix Salviae Miltiorrhizae coarse powder crosses 140 mesh sieves, it is for subsequent use that coarse yam powder crosses 140 mesh sieves, it is for subsequent use that Poria coarse powder crosses 140 mesh sieves, microorganism detection is carried out in the Radix Angelicae Dahuricae, Rhizoma Dioscoreae, Poria, the sampling of Radix Salviae Miltiorrhizae coarse powder, and the Radix Angelicae Dahuricae after qualified, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae, Poria coarse powder carry out micronizing.
C, micronizing: the Radix Angelicae Dahuricae after Micro biological Tests is qualified, Radix Salviae Miltiorrhizae coarse powder WFM100 type ultra micro vibromill are pulverized, working condition is set as: grinding media is bar-shaped, grinding media diameter is 0.3mm, grinding media pack completeness is 50%, Radix Angelicae Dahuricae inventory is 10kg, grinding time is 30min, Radix Salviae Miltiorrhizae inventory is 9kg, grinding time is 30min, Poria inventory is 10kg, and grinding time is 6h, and Rhizoma Dioscoreae dosage is 10kg, grinding time is 30min, and in the Radix Angelicae Dahuricae after pulverizing, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae, Poria micropowders, particle diameter is the fine powder ratio of 6 ~ 12um is 92%.After the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae, Poria micropowders are crossed 200 mesh sieves, the micropowders having crossed sieve is placed in rustless steel CBS series drawer type permanent-magnetic iron expeller and removes individual metal class material.
D, mixture: by Radix Angelicae Dahuricae micropowders 50 parts by weight, Rhizoma Dioscoreae micropowders 50 part, part Poria micropowders 50 parts, Radix Salviae Miltiorrhizae micropowders 50 parts mixing, be placed in EYH-1000A type two-dimensional motion mixer mix and blend, rotation number is 8rpm, shake number is 6rpm, mixing time is 30min, after mixture is even, in pharmaceutical composition, particle diameter is the fine powder ratio of 4 ~ 14um is 93%.
Embodiment 3
---the preparation method of described pharmaceutical composition
A, pretreatment: get Radix Angelicae Dahuricae 100kg, Rhizoma Dioscoreae 50kg, be placed in the silt etc. that SUS304 rustless steel XSG-600 type circulating water washer utilizes the rolling friction removing medical material of Water spray and the cleaning of general water and medical material respectively, drum speed is 12rpm, after medical material foreign material are wherein rejected, choose, cut into slices again, the Radix Angelicae Dahuricae (sheet) after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 65 DEG C, Rhizoma Dioscoreae after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 75 DEG C, then by the Radix Angelicae Dahuricae, Rhizoma Dioscoreae is placed in RXH type hot air circular drying machine respectively, be dried to moisture content≤3% for subsequent use.
Get Poria 50kg, carry out artificial rinsing with flowing water, the Poria through rinsing cleaning cleans, and is rejected by foreign material wherein, after Poria is placed in steam stove and steams to the saturating heart, peel, dice, the Poria after dicing is placed in baking oven sterilizing, sterilization time is set as 9min, sterilising temp is 70 DEG C, then Poria is placed in RXH type hot air circular drying machine, is dried to moisture content≤3% for subsequent use.
B, coarse pulverization: the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Poria Circoplex classified grinding machine are pulverized, it is for subsequent use that Radix Angelicae Dahuricae coarse powder crosses 80 mesh sieves, it is for subsequent use that Radix Salviae Miltiorrhizae coarse powder crosses 80 mesh sieves, it is for subsequent use that coarse yam powder crosses 80 mesh sieves, it is for subsequent use that Poria coarse powder crosses 80 mesh sieves, microorganism detection is carried out in the Radix Angelicae Dahuricae, Rhizoma Dioscoreae, the sampling of Poria coarse powder, and the Radix Angelicae Dahuricae after qualified, Rhizoma Dioscoreae, Poria coarse powder carry out micronizing.
C, micronizing: the Radix Angelicae Dahuricae coarse powder WFM100 type ultra micro vibromill after Micro biological Tests is qualified is pulverized, working condition is set as: grinding media is bar-shaped, grinding media diameter is 0.3mm, and grinding media pack completeness is 50%, and Radix Angelicae Dahuricae inventory is 10kg, grinding time is 30min, Poria inventory is 10kg, and grinding time is 6h, and Rhizoma Dioscoreae dosage is 12kg, grinding time is 30min, and in the Radix Angelicae Dahuricae after pulverizing, Rhizoma Dioscoreae, Poria micropowders, particle diameter is the fine powder ratio of 6 ~ 12um is 92%.After the Radix Angelicae Dahuricae, Rhizoma Dioscoreae, Poria micropowders are crossed 200 mesh sieves, the micropowders having crossed sieve is placed in rustless steel CBS series drawer type permanent-magnetic iron expeller and removes individual metal class material.
D, mixture: by Radix Angelicae Dahuricae micropowders 150 parts by weight, the 50 parts of mixing of Rhizoma Dioscoreae micropowders 50 part, part Poria micropowders, be placed in EYH-1000A type two-dimensional motion mixer mix and blend, rotation number is 8rpm, shake number is 6rpm, mixing time is 30min, after mixture is even, in pharmaceutical composition, particle diameter is the fine powder ratio of 4 ~ 14um is 94%.
Embodiment 4
---the preparation method of described pharmaceutical composition
A, pretreatment: get Radix Angelicae Dahuricae 100kg, Rhizoma Dioscoreae 50kg, be placed in the silt etc. that SUS304 rustless steel XSG-600 type circulating water washer utilizes the rolling friction removing medical material of Water spray and the cleaning of general water and medical material respectively, drum speed is 12rpm, after medical material foreign material are wherein rejected, choose, cut into slices again, the Radix Angelicae Dahuricae (sheet) after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 65 DEG C, Rhizoma Dioscoreae after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 75 DEG C, then by the Radix Angelicae Dahuricae, Rhizoma Dioscoreae is placed in RXH type hot air circular drying machine respectively, be dried to moisture content≤3% for subsequent use.
Get Poria 50kg, carry out artificial rinsing with flowing water, the Poria through rinsing cleaning cleans, and is rejected by foreign material wherein, after Poria is placed in steam stove and steams to the saturating heart, peel, dice, the Poria after dicing is placed in baking oven sterilizing, sterilization time is set as 9min, sterilising temp is 70 DEG C, then Poria is placed in RXH type hot air circular drying machine, is dried to moisture content≤3% for subsequent use.
B, coarse pulverization: the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Poria Circoplex classified grinding machine are pulverized, it is for subsequent use that Radix Angelicae Dahuricae coarse powder crosses 80 mesh sieves, it is for subsequent use that Radix Salviae Miltiorrhizae coarse powder crosses 80 mesh sieves, it is for subsequent use that coarse yam powder crosses 80 mesh sieves, it is for subsequent use that Poria coarse powder crosses 80 mesh sieves, microorganism detection is carried out in the Radix Angelicae Dahuricae, Rhizoma Dioscoreae, the sampling of Poria coarse powder, and the Radix Angelicae Dahuricae after qualified, Rhizoma Dioscoreae, Poria coarse powder carry out micronizing.
C, micronizing: the Radix Angelicae Dahuricae coarse powder WFM100 type ultra micro vibromill after Micro biological Tests is qualified is pulverized, working condition is set as: grinding media is bar-shaped, grinding media diameter is 0.3mm, and grinding media pack completeness is 50%, and Radix Angelicae Dahuricae inventory is 10kg, grinding time is 30min, Poria inventory is 10kg, and grinding time is 6h, and Rhizoma Dioscoreae dosage is 12kg, grinding time is 30min, and in the Radix Angelicae Dahuricae after pulverizing, Rhizoma Dioscoreae, Poria micropowders, particle diameter is the fine powder ratio of 6 ~ 12um is 92%.After the Radix Angelicae Dahuricae, Rhizoma Dioscoreae, Poria micropowders are crossed 200 mesh sieves, the micropowders having crossed sieve is placed in rustless steel CBS series drawer type permanent-magnetic iron expeller and removes individual metal class material.
D, mixture: by Radix Angelicae Dahuricae micropowders 50 parts by weight, the 150 parts of mixing of Rhizoma Dioscoreae micropowders 150 part, part Poria micropowders, be placed in EYH-1000A type two-dimensional motion mixer mix and blend, rotation number is 8rpm, shake number is 6rpm, mixing time is 30min, after mixture is even, in pharmaceutical composition, particle diameter is the fine powder ratio of 4 ~ 14um is 93.5%.
Embodiment 5
---the preparation method of described pharmaceutical composition
A, pretreatment: get Radix Angelicae Dahuricae 50kg, Radix Salviae Miltiorrhizae 50kg, Rhizoma Dioscoreae 50kg, be placed in the silt etc. that SUS304 rustless steel XSG-600 type circulating water washer utilizes the rolling friction removing medical material of Water spray and the cleaning of general water and medical material respectively, drum speed is 12rpm, after medical material foreign material are wherein rejected, choose, cut into slices again, the Radix Angelicae Dahuricae (sheet) after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 65 DEG C, Radix Salviae Miltiorrhizae after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 70 DEG C, Rhizoma Dioscoreae after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 75 DEG C, then by the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae is placed in RXH type hot air circular drying machine respectively, be dried to moisture content≤3% for subsequent use.
Get Poria 50kg, carry out artificial rinsing with flowing water, the Poria through rinsing cleaning cleans, and is rejected by foreign material wherein, after Poria is placed in steam stove and steams to the saturating heart, peel, dice, the Poria after dicing is placed in baking oven sterilizing, sterilization time is set as 9min, sterilising temp is 70 DEG C, then Poria is placed in RXH type hot air circular drying machine, is dried to moisture content≤3% for subsequent use.
B, coarse pulverization: the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Poria, Radix Salviae Miltiorrhizae Circoplex classified grinding machine are pulverized, it is for subsequent use that Radix Angelicae Dahuricae coarse powder crosses 80 mesh sieves, it is for subsequent use that Radix Salviae Miltiorrhizae coarse powder crosses 80 mesh sieves, it is for subsequent use that coarse yam powder crosses 80 mesh sieves, it is for subsequent use that Poria coarse powder crosses 80 mesh sieves, microorganism detection is carried out in the Radix Angelicae Dahuricae, Rhizoma Dioscoreae, Poria, the sampling of Radix Salviae Miltiorrhizae coarse powder, and the Radix Angelicae Dahuricae after qualified, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae, Poria coarse powder carry out micronizing.
C, micronizing: the Radix Angelicae Dahuricae after Micro biological Tests is qualified, Radix Salviae Miltiorrhizae coarse powder WFM100 type ultra micro vibromill are pulverized, working condition is set as: grinding media is bar-shaped, grinding media diameter is 0.3mm, grinding media pack completeness is 50%, Radix Angelicae Dahuricae inventory is 10kg, grinding time is 30min, Radix Salviae Miltiorrhizae inventory is 9kg, grinding time is 30min, Poria inventory is 10kg, and grinding time is 6h, and Rhizoma Dioscoreae dosage is 12kg, grinding time is 30min, and in the Radix Angelicae Dahuricae after pulverizing, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae, Poria micropowders, particle diameter is the fine powder ratio of 6 ~ 12um is 92%.After the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae, Poria micropowders are crossed 200 mesh sieves, the micropowders having crossed sieve is placed in rustless steel CBS series drawer type permanent-magnetic iron expeller and removes individual metal class material.
D, mixture: by Radix Angelicae Dahuricae micropowders 150 parts by weight, Rhizoma Dioscoreae micropowders 100 part, part Poria micropowders 100 parts, Radix Salviae Miltiorrhizae micropowders 50 parts mixing, be placed in EYH-1000A type two-dimensional motion mixer mix and blend, rotation number is 8rpm, shake number is 6rpm, mixing time is 30min, after mixture is even, in pharmaceutical composition, particle diameter is the fine powder ratio of 4 ~ 14um is 91%.
Embodiment 6
---the preparation method of described pharmaceutical composition
A, pretreatment: get Radix Angelicae Dahuricae 50kg, Radix Salviae Miltiorrhizae 50kg, Rhizoma Dioscoreae 50kg, be placed in the silt etc. that SUS304 rustless steel XSG-600 type circulating water washer utilizes the rolling friction removing medical material of Water spray and the cleaning of general water and medical material respectively, drum speed is 12rpm, after medical material foreign material are wherein rejected, choose, cut into slices again, the Radix Angelicae Dahuricae (sheet) after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 65 DEG C, Radix Salviae Miltiorrhizae after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 70 DEG C, Rhizoma Dioscoreae after section is placed in baking oven sterilizing, sterilization time is set as 10min, sterilising temp is 75 DEG C, then by the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae is placed in RXH type hot air circular drying machine respectively, be dried to moisture content≤3% for subsequent use.
Get Poria 50kg, carry out artificial rinsing with flowing water, the Poria through rinsing cleaning cleans, and is rejected by foreign material wherein, after Poria is placed in steam stove and steams to the saturating heart, peel, dice, the Poria after dicing is placed in baking oven sterilizing, sterilization time is set as 9min, sterilising temp is 70 DEG C, then Poria is placed in RXH type hot air circular drying machine, is dried to moisture content≤3% for subsequent use.
B, coarse pulverization: the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Poria, Radix Salviae Miltiorrhizae Circoplex classified grinding machine are pulverized, it is for subsequent use that Radix Angelicae Dahuricae coarse powder crosses 80 mesh sieves, it is for subsequent use that Radix Salviae Miltiorrhizae coarse powder crosses 80 mesh sieves, it is for subsequent use that coarse yam powder crosses 80 mesh sieves, it is for subsequent use that Poria coarse powder crosses 80 mesh sieves, microorganism detection is carried out in the Radix Angelicae Dahuricae, Rhizoma Dioscoreae, Poria, the sampling of Radix Salviae Miltiorrhizae coarse powder, and the Radix Angelicae Dahuricae after qualified, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae, Poria coarse powder carry out micronizing.
C, micronizing: the Radix Angelicae Dahuricae after Micro biological Tests is qualified, Radix Salviae Miltiorrhizae coarse powder WFM100 type ultra micro vibromill are pulverized, working condition is set as: grinding media is bar-shaped, grinding media diameter is 0.3mm, grinding media pack completeness is 50%, Radix Angelicae Dahuricae inventory is 10kg, grinding time is 30min, Radix Salviae Miltiorrhizae inventory is 9kg, grinding time is 30min, Poria inventory is 10kg, and grinding time is 6h, and Rhizoma Dioscoreae dosage is 12kg, grinding time is 30min, and in the Radix Angelicae Dahuricae after pulverizing, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae, Poria micropowders, particle diameter is the fine powder ratio of 6 ~ 12um is 92%.After the Radix Angelicae Dahuricae, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae, Poria micropowders are crossed 200 mesh sieves, the micropowders having crossed sieve is placed in rustless steel CBS series drawer type permanent-magnetic iron expeller and removes individual metal class material.
D, mixture: by Radix Angelicae Dahuricae micropowders 100 parts by weight, Rhizoma Dioscoreae micropowders 50 part, part Poria micropowders 150 parts, Radix Salviae Miltiorrhizae micropowders 100 parts mixing, be placed in EYH-1000A type two-dimensional motion mixer mix and blend, rotation number is 8rpm, shake number is 6rpm, mixing time is 30min, after mixture is even, in pharmaceutical composition, particle diameter is the fine powder ratio of 4 ~ 14um is 91.5%.
Embodiment 7
Pharmaceutical composition pack prepared by Example 1 becomes powder.
Embodiment 8
Pharmaceutical composition prepared by Example 2 adds pharmaceutically acceptable adjuvant and makes tablet.
Embodiment 9
Pharmaceutical composition prepared by Example 3 adds pharmaceutically acceptable adjuvant and makes capsule.
Embodiment 10
Pharmaceutical composition prepared by Example 4 adds pharmaceutically acceptable adjuvant and makes granule.
Embodiment 11
Pharmaceutical composition prepared by Example 5 adds pharmaceutically acceptable adjuvant and makes drop pill.
Embodiment 12
Pharmaceutical composition prepared by Example 5 adds pharmaceutically acceptable adjuvant and makes honey pill agent.
Embodiment 13
Prepared by Example 6 wants compositions to add acceptable adjuvant to make externally dressing.
Embodiment 14
---beautifying whitening effect pharmacological evaluation
Experiment material:
(1) experimental drug and reagent: pharmaceutical composition of the present invention prepared by embodiment 1, Radix Angelicae Dahuricae powder, Rhizoma Dioscoreae powder, Poria powder, Radix Salviae Miltiorrhizae powder, (medicine 1 group is called by weight for the Radix Angelicae Dahuricae powder of 2:1:1:1,1:2:1:1,1:1:2:1,1:1:1:2,1:1:1:1 mixture, Rhizoma Dioscoreae powder, Poria powder, Radix Salviae Miltiorrhizae powder pharmaceutical composition, medicine 2 groups, medicine 3 groups, medicine 4 groups, medicine 5 groups), VitC sheet: 100mg/ sheet, becomes medicinal liquid with distilled water by Pharmaceutical formulations.Progesterone injection: often prop up 10mg/ml (lot number 060314, Zhejiang Province XianJu Pharmacy stock Co., Ltd produces), superoxide dismutase (SOD) measures test kit (Changchun Hui Li Bioisystech Co., Ltd), malonaldehyde (MDA) measures test kit (Changchun Hui Li Bioisystech Co., Ltd)
(2) apparatus is tested
GTZA type precision balance (Kunming networking weighing apparatus corporation) electronic balance (Kunming networking weighing apparatus corporation), constant water bath box (Shanghai is than bright instrument manufacturing company limited).
2, experimental technique
(1) laboratory animal is Kunming mouse 80, adaptability is fed one week, according to completely random principle, mice is divided into 8 groups, be respectively blank group (n=10), vitC group (n=10), model group (n=10), pharmaceutical composition different proportion group (n=10) of the present invention.
(2) after mice normally feeds one week, the estrogenic method of injection in body is adopted to copy mice chloasma model, except blank group, other groups are all injected in mice hind leg muscle with progesterone injection, dosage is 20mg/kg, each treated animal all replaces intramuscular injection in two back legs, once a day, continuous 30 days, after 30 days, skin of back pathological examination is carried out to mice, using human skin tissue HMB45 labelling as positive control, have chloasma sample to change through graphical analysis mouse back skin, and have significant difference with blank group, namely point out modeling success.
(3) after modeling success, while each group of continuation progesterone injection carries out attacking, start to carry out administration to each group of mice, blank group and model group are fed and are given equivalent distilled water, and vitC group is equivalent to the dose,equivalent of people's clinical application by 90.1mg/kg(every day) body weight gavages vitC sheet, and model group is by 8.71g/kg administration every day, once a day, continuous gavage, in the above-mentioned time, the free diet water inlet of each treated animal.
(4) sacrifice of animal sloughs mice dorsal body setae with 8% sodium sulfide solution in first 3 days, and expose one piece of skin, area is 2 × 3cm about.After execution; get each one piece of skin of back, liver organization; use pre-cooling normal saline flushing; Ex-all impurity; filter paper is wiped dry; cut skin, each 0.5g of liver organization, put into respectively and be equipped with 9 times to the small beaker of the pre-cold saline of piece of tissue weight, shred the pulverizing of rear XHF-1 type high speed disperser, homogenate.The centrifugal 15min of homogenate, gets supernatant, carries out index of correlation mensuration.
SOD measures
Measuring principle: generate oxygen molecule by xanthine and xanthine oxidase response system, the latter is oxidized azanol and forms nitrite, aubergine is presented under the effect of developer, its absorbance is surveyed with visible spectrophotometer, when sample is containing SOD, then there is narrow spectrum inhibitory action to ultra-oxygen anion free radical, the nitrite of formation is reduced, measure the absorbance of pipe during colorimetric lower than the absorbance of control tube, the SOD vigor in sample can be obtained by formulae discovery.
The composition of reagent and preparation:
Reagent one: storing solution: 10ml × 1 bottle, adding distil water is diluted to 100ml.
Reagent two: liquid: 10ml × 1 bottle.
Reagent three: liquid: 10ml × 1 bottle.
Reagent four: liquid: 350ul × 2, No. 4 diluents: 10ml × 1 bottle.The two presses 1:14 dilution.
Reagent five: powder pin × 1, dissolves with 70-80 DEG C of hot DDW 75ml.
Reagent six: powder pin × 1, adding distil water 75ml dissolves.
The preparation of developer: according to reagent five: reagent six: the volume ratio preparation of glacial acetic acid=3:3:2.
Operating procedure: (1) is respectively at mensuration pipe and control tube reagent adding one 1.0ml; (2) liver homogenate centrifugal liquid 10ul, Skin Homogenate centrifugal liquid 30ul is added, respectively at control tube adding distil water 10ul, 30ul at mensuration pipe respectively; (3) respectively at mensuration pipe and control tube reagent adding two, reagent three, each 1.0ml of reagent four; (4) fully mix with eddy blending machine, put 37 DEG C of water bath with thermostatic control 40min; (5) add developer 2ml at mensuration pipe and control tube respectively, mixing, room temperature places 10min, and in wavelength 550nm place, 1cm optical path is than colour circle, and distilled water returns to zero, colorimetric.
Computing formula:
The content of albumen in SOD vigor in tissue homogenate=[(control tube absorbance-mensuration pipe absorbance)/control tube absorbance] × 2 × (reactant liquor cumulative volume/sampling amount)/tissue
MDA measures
Measuring principle: the MDA in LPO catabolite can with thiobarbituricacidα-(TBA) condensation, formed red product, have maximum absorption band at 532nm place.
The composition of reagent and preparation:
Reagent one: liquid: 20ml × 1 bottle, adding distil water is diluted to 100ml;
Reagent two: liquid: 12ml × 1 bottle, adds the mixing of 340ml DDW;
Reagent three: powder: 1, adds in the DDW 60ml of 90-100 DEG C by powder, complement to 60ml add glacial acetic acid 60ml again after fully dissolving with DDW, mixing, lucifuge;
Standard substance: 10nmol/ml tetraethoxypropane 5ml × 1 bottle.
Operating procedure:
(1) add 10nmol/ml standard substance 1.0ml at mensuration pipe, add dehydrated alcohol 1.0ml at standard blank tube;
(2) liver homogenate centrifugal liquid 10ul, Skin Homogenate centrifugal liquid 30ul is added at mensuration pipe respectively;
(3) liver homogenate centrifugal liquid 10ul, Skin Homogenate centrifugal liquid 30ul is added at mensuration blank tube respectively;
(4) reagent one 1.0ml is added, mixing at mensuration pipe, standard blank tube, mensuration pipe, mensuration blank tube respectively;
(5) reagent one 3ml is added at mensuration pipe, standard blank tube, mensuration pipe, mensuration blank tube respectively;
(6) add reagent three 1ml at mensuration pipe, standard blank tube, mensuration pipe respectively, add 50% glacial acetic acid 1ml at mensuration blank tube;
(7) eddy blending machine mixing, test tube mouth preservative film is tightened, and stings an aperture with syringe needle, 95 DEG C of water-bath 40min, flowing water cooling after taking out, then 3500 revs/min, centrifugal 10min, gets supernatant, 532nm place, 1cm optical path cuvette, distilled water returns to zero, and surveys each pipe absorbance.
(8) computing formula: protein content in MDA content=(measuring pipe absorbance-mensuration blank tube absorbance)/(standard pipe absorbance-standard blank tube absorbance) × standard concentration/tissue in tissue
3, experimental result and analysis
Table one mouse liver SOD, MDA content (x ± s)
Note: compare with blank group,
△p<0.05,
△ △p<0.01; Compare with model group,
*p<0.05
*p<0.01.
From table one: compare with blank group, model group mouse liver SOD activity reduces, and MDA content raises, and wherein MDA has significant difference (P<0.01).Pharmaceutical composition (1:2:1:1), pharmaceutical composition (1:1:1:2) are organized SOD activity and are raised, and compare have significant difference with model group, and pharmaceutical composition (1:1:1:1) organizes the active obviously rising of SOD, remarkable with the poor heteropole of model group.For MDA content, compare with model group, pharmaceutical composition (1:1:1:1), pharmaceutical composition (1:1:1:2) organizes MDA activity pole significant difference, in sum, mouse liver SOD content all increases pharmaceutical composition of the present invention upon administration, MDA content all reduces, and wherein to organize effect the most remarkable for pharmaceutical composition (1:1:1:1).
Table two mouse skin SOD, MDA content (x ± s)
Note: compare with blank group,
△p<0.05,
△ △p<0.01; Compare with model group,
*p<0.05
*p<0.01.
From table two: for SOD content, compare with model group, pharmaceutical composition (1:1:1:1), pharmaceutical composition (2:1:1:1), pharmaceutical composition (1:2:1:1), pharmaceutical composition (1:1:1:2) group increases all to some extent, and pharmaceutical composition (1:1:1:1) has pole significant difference, compare with blank group, combinations thereof SOD content increases all to some extent, and pharmaceutical composition (1:1:1:1) has pole significant difference, and for MDA content, pharmaceutical composition (1:1:1:1), pharmaceutical composition (2:1:1:1), pharmaceutical composition (1:2:1:1), pharmaceutical composition (1:1:2:1) declines all to some extent, wherein to organize effect the most obvious for pharmaceutical composition of the present invention (1:1:1:1).In sum, pharmaceutical composition of the present invention (1:1:1:1) group is the most remarkable on the impact of mouse skin SOD, MDA content.
Toxicity test
Utilize rabbit to carry out acute irritation experiment, by the eyes of sample aqueous solution white rabbit side, contralateral eye instillation distilled water compares, and observing respectively at 6h, 24h, 48h after a medicine, is 0 after average eye irritation 48h.Pharmaceutical composition avirulence of the present invention and untoward reaction are described, use safety.
Embodiment 15
The pharmaceutical composition prepared with embodiment 2, embodiment 3, embodiment 4, embodiment 5 and embodiment 6 is respectively tested, test method leads to embodiment 14, and result all shows pharmaceutical composition that the present invention prepares on the impact of mouse skin SOD, MDA content significantly.
Claims (9)
1. a pharmaceutical composition, is characterized in that described pharmaceutical composition comprises the Radix Angelicae Dahuricae, Rhizoma Dioscoreae and Poria, and weight ratio is 1 ~ 3:1 ~ 3:1 ~ 3.
2. pharmaceutical composition according to claim 1, is characterized in that described pharmaceutical composition also comprises Radix Salviae Miltiorrhizae, and the weight ratio of the Radix Angelicae Dahuricae in described pharmaceutical composition, Rhizoma Dioscoreae, Poria and Radix Salviae Miltiorrhizae is 1 ~ 3:1 ~ 3:1 ~ 3:1 ~ 3.
3. pharmaceutical composition according to claim 2, it is characterized in that the Radix Angelicae Dahuricae in described pharmaceutical composition, Rhizoma Dioscoreae, Poria and Radix Salviae Miltiorrhizae weight ratio be 2:2:2:2.
4. a preparation method for the arbitrary described pharmaceutical composition of claim 1 ~ 3, is characterized in that comprising pretreatment, coarse pulverization, micronizing and mixture operation, specifically comprises:
A, pretreatment: the foreign material in each raw material are rejected, through selected, cleaning, dry in the sun, to be dried to moisture content≤3% for subsequent use;
B, coarse pulverization: the raw material Radix Angelicae Dahuricae, Rhizoma Dioscoreae, Poria and Radix Salviae Miltiorrhizae are pulverized with Roughpulverizer respectively, crosses 80 ~ 140 mesh sieves for subsequent use;
C, micronizing: the micropowders each raw material after coarse pulverization being obtained respectively each raw material through micronizing;
D, mixture: each raw material micropowders mixture is evenly obtained object by formulation ratio.
5. the preparation method of pharmaceutical composition according to claim 4, is characterized in that the particle diameter of described micropowders is 6 ~ 12 μm.
6. a preparation for the arbitrary described pharmaceutical composition of claim 1 ~ 3, is characterized in that adding pharmaceutically acceptable adjuvant in described pharmaceutical composition makes powder, tablet, granule, capsule, drop pill, honey pill agent or externally dressing.
7. an application for the arbitrary described pharmaceutical composition of claim 1 ~ 3, is characterized in that described pharmaceutical composition is preparing the application in beauty face-whitening-nourishing medicine or cosmetics.
8. the application of pharmaceutical composition according to claim 7, it is characterized in that described pharmaceutical composition instructions of taking is for take with boiled water or Mel, three times on the one, taking dose is 2g/ time.
9. the application of pharmaceutical composition according to claim 7, is characterized in that adding in described pharmaceutical composition the Chinese liquor external application 15 ~ 25min of Mel, milk or 40 ~ 60 degree.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106860700A (en) * | 2017-02-27 | 2017-06-20 | 普洱淞茂滇草六味制药股份有限公司 | A kind of dispelling speckles and whitening face pharmaceutical composition and preparation method thereof, preparation and application |
| CN106860764A (en) * | 2017-02-27 | 2017-06-20 | 普洱淞茂滇草六味制药股份有限公司 | A kind of whitening repair medicine composition and preparation method thereof, preparation and application |
| CN110215497A (en) * | 2019-06-28 | 2019-09-10 | 甘肃中医药大学 | A kind of skin repair cream and its preparation process |
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| CN103655391A (en) * | 2012-09-25 | 2014-03-26 | 徐巧勤 | Preparation method of face application agent |
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| CN103655391A (en) * | 2012-09-25 | 2014-03-26 | 徐巧勤 | Preparation method of face application agent |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106860700A (en) * | 2017-02-27 | 2017-06-20 | 普洱淞茂滇草六味制药股份有限公司 | A kind of dispelling speckles and whitening face pharmaceutical composition and preparation method thereof, preparation and application |
| CN106860764A (en) * | 2017-02-27 | 2017-06-20 | 普洱淞茂滇草六味制药股份有限公司 | A kind of whitening repair medicine composition and preparation method thereof, preparation and application |
| CN110215497A (en) * | 2019-06-28 | 2019-09-10 | 甘肃中医药大学 | A kind of skin repair cream and its preparation process |
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