CN105348406A - Extraction and purification method for fistular onion stalk polysaccharide - Google Patents
Extraction and purification method for fistular onion stalk polysaccharide Download PDFInfo
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- CN105348406A CN105348406A CN201510826476.5A CN201510826476A CN105348406A CN 105348406 A CN105348406 A CN 105348406A CN 201510826476 A CN201510826476 A CN 201510826476A CN 105348406 A CN105348406 A CN 105348406A
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Abstract
The invention discloses an extraction and purification method for fistular onion stalk polysaccharide. The method comprises the following steps: crushing fistular onion stalk; adding sterile water, mixing the sterile water with the crushed fistular onion stalk and then boiling the obtained mixture; carrying out ultrasonic extraction so as to obtain polysaccharide extract; alcoholizing the polysaccharide extract with edible alcohol with a temperature of 4 to 25 DEG C, carrying out precipitation so as to obtain a polysaccharide solution, then adding the edible alcohol with a temperature of 4 to 25 DEG C again, and carrying out precipitation a plurality of times so as to obtain crude polysaccharide; subjecting the crude polysaccharide to concentration and extraction and adding a Sevag reagent with a volume of 1/3 for deproteinization so as to obtain a deproteinized polysaccharide solution; continuing concentration and alcoholization of the deproteinized polysaccharide solution and carrying out centrifugation so as to obtain polysaccharide; adding distilled water again, mixing the distilled water with the obtained polysaccharide and carrying out filtering with an ultrafilter membrane so as to obtain a polysaccharide component with a molecular weight of 10, 000 Daltons; carrying out column chromatography at a temperature below 25 DEG C so as to obtain purified polysaccharide; and subjecting the purified polysaccharide to freeze drying so as to obtain the fistular onion bulb polysaccharide. The extraction and purification method adopts the advantages of both a water-extraction alcohol-precipitation method and membrane separation technology, discards the disadvantage of difficulty in fusion of high quality and low cost of a single method and is capable of maintaining small structural destroy, high purity and high extraction yield of the polysaccharide, achieving multistage separation effect and reducing energy consumption and processing cost.
Description
Technical field
The present invention relates to biological technical field, particularly relate to a kind of method of extraction purification polysaccharide from Bulbus Allii Fistulosi.
Background technology
Green onion is a kind of very general seasonings and the vegetables of China, and belonging to Amaryllidaceae allium, is perennial root herbaceous plant.Green onion resource is the large cash crop resource of China, green onion industry is the strong industry of China, there is good development prospect in International Vegetable Trade, the current main exit of Cong Shi China is earned foreign exchange vegetables, green onion, ginger and garlic export volume accounts for 1/4 of China's Vegetables Exportation total amount, accounts for world's green onion, ginger and garlic export trade amount more than 70%.China, in green onion industry development, occupies the unique advantage such as industrial scale, manpower, is not available for other countries.Green onion produces and belongs to work and technology-intensive industries.In recent years, most developed countries Vegetable produce weakens, and import increases.Therefore, global vegetables import and export volume will constantly increase, and China is traditional green onion export State, and human resources are sufficient, and the production advantage is obvious, for the development of China's green onion industry provides good opportunity.
Bulbus Allii Fistulosi is the bulb of plant green onion, wherein containing polysaccharose substance, but not yet develops Bulbus Allii Fistulosi polysaccharide up to now both at home and abroad.Traditionally Bulbus Allii Fistulosi utilize by Chinese materia medica, wherein containing volatile oil, VITAMIN and polyose, in dry Bulbus Allii Fistulosi, polysaccharide content is 10% ~ 20%, and therefore the exploitation of Bulbus Allii Fistulosi polysaccharide have feasibility.And the physiological function of vegetable polysaccharides is comparatively extensive, according to document announcement polysaccharide, there is immunity moderation, hypoglycemic, reducing blood-fat, the anti-ageing effect of waiting for a long time.Along with the raising of socioeconomic development and living standards of the people and the reinforcement of individual health care consciousness, functional health care food is more and more paid close attention to by human consumer.
Vegetable polysaccharides product is a lot of in the market, but there are no the product of Bulbus Allii Fistulosi polysaccharide, does not more have the special extracting and purifying method for Bulbus Allii Fistulosi polysaccharide.
Summary of the invention
The invention provides a kind of Bulbus Allii Fistulosi Polyose extraction purification process, it takes the strong point of water extraction and alcohol precipitation method and membrane separation technique, abandon the shortcoming that the high-quality of single method and low cost are difficult to merge, this technology can keep polysaccharide structures destroy less, purity is high, extraction yield is high, reach the effect of stage trapping, energy consumption can be reduced again and cut down finished cost.
The present invention adopts following technical scheme: a kind of Bulbus Allii Fistulosi Polyose extraction purification process, comprises the steps:
(1) the chive Bulbus Allii Fistulosi of drying is carried out pulverizing and sieving, obtain chive Bulbus Allii Fistulosi powder;
(2) get chive Bulbus Allii Fistulosi powder, mixed by chive Bulbus Allii Fistulosi powder, heated and boiled 2-3 hour, and be aided with ultrasonic-assisted extraction with sterilized water with 1:5-1:8 ratio, at least centrifugal 10-15 minute, gets supernatant liquor; Residue after centrifugal extracts at least 3-4 time as stated above again, and supernatant liquor merges, and obtains polysaccharide extraction liquid;
(3) adopt Rotary Evaporators, at 60-80 DEG C, polysaccharide extraction liquid is concentrated into 1/4 ~ 1/7 of original volume, obtain polysaccharide concentrated solution;
(4) with 4-25 DEG C of edible ethanol precipitate polysaccharides concentrated solution, make ethanol content reach cumulative volume 65-80%, polysaccharide is separated out with precipitation forms, water, partial impurities and pigmentolysis alcohol mutually in, at least centrifugal 10-15 minute, gets precipitated phase, dissolve with alcohol again, repeat alcohol precipitation at least 2-3 time;
(5) be fully dissolved in distilled water by alcohol precipitation Crude polysaccharides, the pH value adjusting this Crude polysaccharides solution by Na2CO3 dilute solution is 4.5-7.6, adds trypsinase, volume of toluene 1-2%, 35-37 DEG C of constant temperature hydrolysis certain hour, and rotary evaporation concentrates;
(6) concentrated solution after proteolysis is added the Sevag reagent accounting for volume 1/3, extracting 1-3 hour, centrifugal 10-15 minute, gets supernatant liquor; Supernatant liquor continues to carry out deproteinated with the Sevag reagent of 1/3 volume, repeats at least 2-3 time;
(7) deproteinated polysaccharide liquid is concentrated into original volume 1/3-1/4,4-25 DEG C of more than alcohol precipitation 24h, at least centrifugal 10 15min, Qi Shang Cheongju liquid is dry ethanol and room temperature is volatilized, and obtains polysaccharide;
(8) dissolve with polysaccharide quality 5-15 distilled water doubly, utilize ultra-filtration membrane that Bulbus Allii Fistulosi polysaccharide extraction liquid is carried out ultrafiltration, collect the component of more than molecular weight 10000 dalton, carry out dextrane gel SephadexG-100 again to carry out column chromatography in less than 25 degree and obtain purified polysaccharide, collect polysaccharide fraction, lyophilize, obtains Bulbus Allii Fistulosi polysaccharide.
Further improvement of the present invention is, the speed of step (2), step (4) and the described centrifugal extraction of step (6) is 8,000 10000 revs/min.
Further improvement of the present invention is, sieving described in step (1) was 80-120 mesh sieve.
Further improvement of the present invention is, the mixed solution that the chloroform of the described Sevag reagent of step (6) to be volume ratio be 4:1 and propyl carbinol are mixed with.
Further improvement of the present invention is, the pH value of the described Crude polysaccharides solution of step (5) is adjusted to 7.5, and described tryptic activity is 8000-10000U/g.
Further improvement of the present invention is, the ethanol of step (7) described ethanol to be concentration be volume percent 90-98%.Preferably, the ethanol of step (7) described ethanol to be concentration be volume percent 95%.
The present invention compared with prior art has following advantage and beneficial effect:
(1) extracting method product recovery rate of the present invention is high, purity good, and Bulbus Allii Fistulosi polysaccharide yield is greater than 2.0%, and the purity of polysaccharide is greater than 90.0%.
(2) present invention process is simple, and agents useful for same is easy to get, and production cost is low, is easy to realize industrialization.
Accompanying drawing explanation
The process flow sheet of an embodiment of a kind of Bulbus Allii Fistulosi Polyose extraction of Fig. 1 purification process.
Embodiment
In order to deepen the understanding of the present invention, below in conjunction with drawings and Examples, the present invention is further described, and this embodiment, only for explaining the present invention, not forming protection scope of the present invention and limiting.
As shown in Figure 1, a kind of Bulbus Allii Fistulosi Polyose extraction purification process, comprises the steps:
(1) the chive Bulbus Allii Fistulosi of drying is carried out pulverizing and sieving, obtain chive Bulbus Allii Fistulosi powder, see 101 in Fig. 1;
(2) get chive Bulbus Allii Fistulosi powder, mixed by chive Bulbus Allii Fistulosi powder, heated and boiled 2-3 hour, and be aided with ultrasonic-assisted extraction with sterilized water with 1:5-1:8 ratio, at least centrifugal 10-15 minute, gets supernatant liquor; Residue after centrifugal extracts at least 3-4 time as stated above again, and supernatant liquor merges, and obtains polysaccharide extraction liquid, see 102 in Fig. 1;
(3) adopt Rotary Evaporators, at 60-80 DEG C, polysaccharide extraction liquid is concentrated into 1/4 ~ 1/7 of original volume, obtain polysaccharide concentrated solution, see 103 in Fig. 1;
(4) with 4-25 DEG C of edible ethanol precipitate polysaccharides concentrated solution, ethanol content is made to reach cumulative volume 65-80%, polysaccharide is separated out with precipitation forms, water, partial impurities and pigmentolysis alcohol mutually in, at least centrifugal 10-15 minute, gets precipitated phase, then dissolves with alcohol, repeat alcohol precipitation at least 2-3 time, see 104 in Fig. 1;
(5) be fully dissolved in distilled water by alcohol precipitation Crude polysaccharides, the pH value adjusting this Crude polysaccharides solution by Na2CO3 dilute solution is 4.5-7.6, adds trypsinase, volume of toluene 1-2%, 35-37 DEG C of constant temperature hydrolysis certain hour, and rotary evaporation concentrates, see 105 in Fig. 1;
(6) concentrated solution after proteolysis is added the Sevag reagent accounting for volume 1/3, extracting 1-3 hour, centrifugal 10-15 minute, gets supernatant liquor; Supernatant liquor continues to carry out deproteinated with the Sevag reagent of 1/3 volume, repeats at least 2-3 time, see 106 in Fig. 1;
(7) deproteinated polysaccharide liquid is concentrated into original volume 1/3-1/4,4-25 DEG C of more than alcohol precipitation 24h, at least centrifugal 10 15min, Qi Shang Cheongju liquid is dry ethanol and room temperature is volatilized, and obtains polysaccharide, see 107 in Fig. 1;
(8) dissolve with polysaccharide quality 5-15 distilled water doubly, utilize ultra-filtration membrane that Bulbus Allii Fistulosi polysaccharide extraction liquid is carried out ultrafiltration, collect the component of more than molecular weight 10000 dalton, carry out dextrane gel SephadexG-100 again to carry out column chromatography in less than 25 degree and obtain purified polysaccharide, collect polysaccharide fraction, lyophilize, obtains Bulbus Allii Fistulosi polysaccharide, see 108 in Fig. 1.
In the above-described embodiments, the speed of step (2), step (4) and step (6) centrifugal extraction is 8000
?10000 revs/min.It was 80-120 mesh sieve that step (1) is sieved.The mixed solution that the chloroform of step (6) Sevag reagent to be volume ratio be 4:1 and propyl carbinol are mixed with.The pH value of step (5) Crude polysaccharides solution is adjusted to 7.5, and tryptic activity is 8000-10000U/g.The preferred alcohol concn of ethanol of step (7) ethanol to be concentration be volume percent 90-98% is volume percent 95%.
embodiment 1
(1) the chive Bulbus Allii Fistulosi of drying is carried out pulverizing 80 orders to sieve, obtain chive Bulbus Allii Fistulosi powder;
(2) get chive Bulbus Allii Fistulosi powder, chive Bulbus Allii Fistulosi powder is mixed with 1:5 ratio with sterilized water, heated and boiled 2 hours, and be aided with ultrasonic-assisted extraction, centrifugal 10 minutes, get supernatant liquor; Residue after centrifugal extracts 3 times as stated above again, and supernatant liquor merges, and obtains polysaccharide extraction liquid;
(3) adopt Rotary Evaporators, at 60 DEG C, polysaccharide extraction liquid is concentrated into 1/4 of original volume, obtain polysaccharide concentrated solution;
(4) with 4 DEG C of edible ethanol precipitate polysaccharides concentrated solutions, make ethanol content reach cumulative volume 65%, polysaccharide is separated out with precipitation forms, water, partial impurities and pigmentolysis alcohol mutually in, centrifugal 10 minutes, get precipitated phase, then dissolve with alcohol, repeat alcohol precipitation 2 times;
(5) be fully dissolved in distilled water by alcohol precipitation Crude polysaccharides, be 7.5, add trypsinase with Na2CO3 dilute solution adjust pH, volume 1% toluene, 37 DEG C of constant temperature hydrolysis certain hours, rotary evaporation concentrates;
(6) concentrated solution after proteolysis is added the Sevag reagent accounting for volume 1/3, extracting 1 hour, centrifugal 10 minutes, get supernatant liquor; Supernatant liquor continues to carry out deproteinated with the Sevag reagent of 1/3 volume, repeats 2 times;
(7) deproteinated polysaccharide liquid is concentrated into original volume 1/3,4 DEG C of alcohol precipitation 24h, centrifugal 10min, Qi Shang Cheongju liquid is dry ethanol and room temperature is volatilized, and obtains polysaccharide;
(8) dissolve with the distilled water of polysaccharide quality 5 times, utilize ultra-filtration membrane that Bulbus Allii Fistulosi polysaccharide extraction liquid is carried out ultrafiltration, collect the component of more than molecular weight 10000 dalton, carry out SephadexG-100 again at 25 DEG C, to carry out column chromatography obtain purified polysaccharide, collect polysaccharide fraction, lyophilize, obtains Bulbus Allii Fistulosi polysaccharide.
Bulbus Allii Fistulosi polysaccharide prepared by the present embodiment is filbert, utilizes the conventional drying methods such as baking oven cannot be dry, and utilize lyophilize just can obtain powder, easy moisture absorption, soluble in water, mass content is 90.8%.Polysaccharide content detects: accurately take Bulbus Allii Fistulosi polysaccharide 10mg in 100mL beaker, fully dissolve, and draws polysaccharide soln 0.5mL, add 5% phenol 1.0mL and vitriol oil 3.0mL, static 10min, shakes up, room temperature places 15min, take distilled water as blank, in 490nm place side draught light value.
embodiment 2
(1) the chive Bulbus Allii Fistulosi of drying is carried out pulverizing 100 orders to sieve, obtain chive Bulbus Allii Fistulosi powder;
(2) get chive Bulbus Allii Fistulosi powder, chive Bulbus Allii Fistulosi powder is mixed with 1:6 ratio with sterilized water, heated and boiled 2.5 hours, and be aided with ultrasonic-assisted extraction, centrifugal 10 minutes, get supernatant liquor; Residue after centrifugal extracts 3 times as stated above again, and supernatant liquor merges, and obtains polysaccharide extraction liquid;
(3) adopt Rotary Evaporators, at 70 DEG C, polysaccharide extraction liquid is concentrated into 15 of original volume, obtain polysaccharide concentrated solution;
(4) with 4 DEG C of edible ethanol precipitate polysaccharides concentrated solutions, make ethanol content reach cumulative volume 70%, polysaccharide is separated out with precipitation forms, water, partial impurities and pigmentolysis alcohol mutually in, centrifugal 10 minutes, get precipitated phase, then dissolve with alcohol, repeat alcohol precipitation 2 times;
(5) be fully dissolved in distilled water by alcohol precipitation Crude polysaccharides, be 7.5, add trypsinase with Na2CO3 dilute solution adjust pH, volume 1.5% toluene, 37 DEG C of constant temperature hydrolysis certain hours, rotary evaporation concentrates;
(6) concentrated solution after proteolysis is added the Sevag reagent accounting for volume 1/3, extracting 2 hours, centrifugal 10 minutes, get supernatant liquor; Supernatant liquor continues to carry out deproteinated with the Sevag reagent of 1/3 volume, repeats 2 times;
(7) deproteinated polysaccharide liquid is concentrated into original volume 1/4,25 DEG C of alcohol precipitation 30h, centrifugal 10min, Qi Shang Cheongju liquid is dry ethanol and room temperature is volatilized, and obtains polysaccharide;
(8) dissolve with the distilled water of polysaccharide quality 10 times, utilize ultra-filtration membrane that Bulbus Allii Fistulosi polysaccharide extraction liquid is carried out ultrafiltration, collect the component of more than molecular weight 10000 dalton, carry out SephadexG-100 again at 25 DEG C, to carry out column chromatography obtain purified polysaccharide, collect polysaccharide fraction, lyophilize, obtains Bulbus Allii Fistulosi polysaccharide.
Bulbus Allii Fistulosi polysaccharide prepared by the present embodiment is filbert, utilizes the conventional drying methods such as baking oven cannot be dry, and utilize lyophilize just can obtain powder, easy moisture absorption, soluble in water, mass content is 90.2%.Polysaccharide content detects: accurately take Bulbus Allii Fistulosi polysaccharide 10mg in 100mL beaker, fully dissolve, and draws polysaccharide soln 0.5mL, add 5% phenol 1.0mL and vitriol oil 3.0mL, static 10min, shakes up, room temperature places 15min, take distilled water as blank, in 490nm place side draught light value.
embodiment 3
(1) the chive Bulbus Allii Fistulosi of drying is carried out pulverizing 120 orders to sieve, obtain chive Bulbus Allii Fistulosi powder;
(2) get chive Bulbus Allii Fistulosi powder, chive Bulbus Allii Fistulosi powder is mixed with 1:8 ratio with sterilized water, heated and boiled 3 hours, and be aided with ultrasonic-assisted extraction, centrifugal 15 minutes, get supernatant liquor; Residue after centrifugal extracts 4 times as stated above again, and supernatant liquor merges, and obtains polysaccharide extraction liquid;
(3) adopt Rotary Evaporators, at 80 DEG C, polysaccharide extraction liquid is concentrated into 1/7 of original volume, obtain polysaccharide concentrated solution;
(4) with 25 DEG C of edible ethanol precipitate polysaccharides concentrated solutions, make ethanol content reach cumulative volume 80%, polysaccharide is separated out with precipitation forms, water, partial impurities and pigmentolysis alcohol mutually in, centrifugal 15 minutes, get precipitated phase, then dissolve with alcohol, repeat alcohol precipitation 3 times;
(5) be fully dissolved in distilled water by alcohol precipitation Crude polysaccharides, be 7.5, add trypsinase with Na2CO3 dilute solution adjust pH, volume 2% toluene, 37 DEG C of constant temperature hydrolysis certain hours, rotary evaporation concentrates;
(6) concentrated solution after proteolysis is added the Sevag reagent accounting for volume 1/3, extracting 3 hours, centrifugal 15 minutes, get supernatant liquor; Supernatant liquor continues to carry out deproteinated with the Sevag reagent of 1/3 volume, repeats 3 times;
(7) deproteinated polysaccharide liquid is concentrated into original volume 1/4,25 DEG C of alcohol precipitation 36h, centrifugal 15min, Qi Shang Cheongju liquid is dry ethanol and room temperature is volatilized, and obtains polysaccharide;
(8) dissolve with the distilled water of polysaccharide quality 15 times, utilize ultra-filtration membrane that Bulbus Allii Fistulosi polysaccharide extraction liquid is carried out ultrafiltration, collect the component of more than molecular weight 10000 dalton, carry out SephadexG-100 again at 25 DEG C, to carry out column chromatography obtain purified polysaccharide, collect polysaccharide fraction, lyophilize, obtains Bulbus Allii Fistulosi polysaccharide.
Bulbus Allii Fistulosi polysaccharide prepared by the present embodiment is filbert, utilizes the conventional drying methods such as baking oven cannot be dry, and utilize lyophilize just can obtain powder, easy moisture absorption, soluble in water, mass content is 92.7%.Polysaccharide content detects: accurately take Bulbus Allii Fistulosi polysaccharide 10mg in 100mL beaker, fully dissolve, and draws polysaccharide soln 0.5mL, add 5% phenol 1.0mL and vitriol oil 3.0mL, static 10min, shakes up, room temperature places 15min, take distilled water as blank, in 490nm place side draught light value.
What embodiments of the invention were announced is preferred embodiment, but is not limited thereto, those of ordinary skill in the art; very easily according to above-described embodiment, understand spirit of the present invention, and make different amplifications and change; but only otherwise depart from spirit of the present invention, all in protection scope of the present invention.
Claims (7)
1. a Bulbus Allii Fistulosi Polyose extraction purification process, is characterized in that, comprises the steps:
(1) the chive Bulbus Allii Fistulosi of drying is carried out pulverizing and sieving, obtain chive Bulbus Allii Fistulosi powder;
(2) get chive Bulbus Allii Fistulosi powder, mixed by chive Bulbus Allii Fistulosi powder, heated and boiled 2-3 hour, and be aided with ultrasonic-assisted extraction with sterilized water with 1:5-1:8 ratio, at least centrifugal 10-15 minute, gets supernatant liquor; Residue after centrifugal extracts at least 3-4 time as stated above again, and supernatant liquor merges, and obtains polysaccharide extraction liquid;
(3) adopt Rotary Evaporators, at 60-80 DEG C, polysaccharide extraction liquid is concentrated into 1/4 ~ 1/7 of original volume, obtain polysaccharide concentrated solution;
(4) with 4-25 DEG C of edible ethanol precipitate polysaccharides concentrated solution, make ethanol content reach cumulative volume 65-80%, polysaccharide is separated out with precipitation forms, water, partial impurities and pigmentolysis alcohol mutually in, at least centrifugal 10-15 minute, gets precipitated phase, dissolve with alcohol again, repeat alcohol precipitation at least 2-3 time;
(5) be fully dissolved in distilled water by alcohol precipitation Crude polysaccharides, the pH value adjusting this Crude polysaccharides solution by Na2CO3 dilute solution is 4.5-7.6, adds trypsinase, volume of toluene 1-2%, 35-37 DEG C of constant temperature hydrolysis certain hour, and rotary evaporation concentrates;
(6) concentrated solution after proteolysis is added the Sevag reagent accounting for volume 1/3, extracting 1-3 hour, centrifugal 10-15 minute, gets supernatant liquor; Supernatant liquor continues to carry out deproteinated with the Sevag reagent of 1/3 volume, repeats at least 2-3 time;
(7) deproteinated polysaccharide liquid is concentrated into original volume 1/3-1/4,4-25 DEG C of more than alcohol precipitation 24h, at least centrifugal 10 15min, Qi Shang Cheongju liquid is dry ethanol and room temperature is volatilized, and obtains polysaccharide;
(8) dissolve with polysaccharide quality 5-15 distilled water doubly, utilize ultra-filtration membrane that Bulbus Allii Fistulosi polysaccharide extraction liquid is carried out ultrafiltration, collect the component of more than molecular weight 10000 dalton, carry out dextrane gel SephadexG-100 again to carry out column chromatography in less than 25 degree and obtain purified polysaccharide, collect polysaccharide fraction, lyophilize, obtains Bulbus Allii Fistulosi polysaccharide.
2. extracting and purifying method according to claim 1, is characterized in that: the speed of step (2), step (4) and the described centrifugal extraction of step (6) is 8,000 10000 revs/min.
3. extracting and purifying method according to claim 1, is characterized in that: sieving described in step (1) was 80-120 mesh sieve.
4. extracting and purifying method according to claim 1 or 2, is characterized in that: the mixed solution that the chloroform of the described Sevag reagent of step (6) to be volume ratio be 4:1 and propyl carbinol are mixed with.
5. extracting and purifying method according to claim 1, is characterized in that: the pH value of the described Crude polysaccharides solution of step (5) is adjusted to 7.5, and described tryptic activity is 8000-10000U/g.
6. extracting and purifying method according to claim 1, is characterized in that: the ethanol of step (7) described ethanol to be concentration be volume percent 90-98%.
7. extracting and purifying method according to claim 6, is characterized in that: the ethanol of step (7) described ethanol to be concentration be volume percent 95%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106491387A (en) * | 2016-12-06 | 2017-03-15 | 广州聚禅现代农业研究院有限公司 | A kind of skin Caring facial milk cleanser containing chive polysaccharide |
| CN108329397A (en) * | 2018-03-08 | 2018-07-27 | 西北农林科技大学 | A method of addition inorganic salts improve Hubei Sorbus alnifloria leaf polyose alcohol precipitation efficiency |
| CN111978425A (en) * | 2020-09-03 | 2020-11-24 | 中国农业大学 | Extraction method of food-grade industrialized fistular onion stalk polysaccharide |
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| JPH10158306A (en) * | 1996-11-28 | 1998-06-16 | Fukui Pref Gov | Production of fructan as water-soluble dietary fiber |
| CN104277135A (en) * | 2014-09-23 | 2015-01-14 | 华南农业大学 | Pleurotus tuber-regium polysaccharide and extraction method thereof |
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| JPH10158306A (en) * | 1996-11-28 | 1998-06-16 | Fukui Pref Gov | Production of fructan as water-soluble dietary fiber |
| CN104277135A (en) * | 2014-09-23 | 2015-01-14 | 华南农业大学 | Pleurotus tuber-regium polysaccharide and extraction method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106491387A (en) * | 2016-12-06 | 2017-03-15 | 广州聚禅现代农业研究院有限公司 | A kind of skin Caring facial milk cleanser containing chive polysaccharide |
| CN108329397A (en) * | 2018-03-08 | 2018-07-27 | 西北农林科技大学 | A method of addition inorganic salts improve Hubei Sorbus alnifloria leaf polyose alcohol precipitation efficiency |
| CN111978425A (en) * | 2020-09-03 | 2020-11-24 | 中国农业大学 | Extraction method of food-grade industrialized fistular onion stalk polysaccharide |
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