CN105343125A - Microbial ecological agent for preventing and treating piglet diarrhea and preparation method of microbial ecological agent - Google Patents
Microbial ecological agent for preventing and treating piglet diarrhea and preparation method of microbial ecological agent Download PDFInfo
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- CN105343125A CN105343125A CN201510740876.4A CN201510740876A CN105343125A CN 105343125 A CN105343125 A CN 105343125A CN 201510740876 A CN201510740876 A CN 201510740876A CN 105343125 A CN105343125 A CN 105343125A
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- microbial ecological
- ecological agent
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- bacterium liquid
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Abstract
The invention discloses a microbial ecological agent for preventing and treating piglet diarrhea and a preparation method of the microbial ecological agent. The microbial ecological agent is prepared from pig manure, is used for preventing and treating piglet diarrhea, and has obvious effects, and thus the use amount of an antibiotic for the pig is reduced in the prevention and treatment of the piglet diarrhea. The preparation method comprises the following steps: firstly collecting the manure of the healthy fragrant pig from the Congjiang County of Guizhou Province, adding the manure into stroke-physiological saline solution to prepare a suspension, filtering the suspension with a stainless steel tea hopper and a cell sieve (the hole diameter is 0.224mm) sequentially, thus obtaining the manure bacterial liquid of the pig, adding a strain protection agent to the manure bacterial liquid of the pig, and uniformly mixing, and thus obtaining the microbial ecological agent, wherein 10<3>-10<10> viable organisms exist in each milliliter of the microbial ecological agent. During use, the microbial ecological agent is drenched to the stomach of the piglet, wherein the drenching dosage is 1-10 ml each time, once per day. The preparation process of the microbial ecological agent is simple, the production cost is relatively low, the microbial ecological agent has the significant effect for preventing and treating piglet diarrhea, and the growth performance of the piglet is not influenced.
Description
Technical field
The present invention relates to the biological preparation of control piglet diarrhea, refer to a kind of microbial ecological agent preventing and treating piglet diarrhea and preparation method thereof particularly.
Background technology
Piglet, due to developing immune system imperfection, is easily suffered from diarrhoea when being subject to environmental stimuli.Especially the early weaning measure of piglet can stimulate piglet from aspects such as nutrition, psychology and environment, causes piglet generation Early weaned stress, causes piglet that diarrhoea occurs even dead time serious.Widely use antibiosis in production practices and usually alleviate piglet diarrhea, but becoming increasingly conspicuous along with problems such as antibiotic drug resistance and food safetys, forbidding antibiotic has become a kind of inexorable trend.
The conventional microbial ecological agent be made up of probiotic bacteria carrys out the antibiotic use of Some substitute pig at present.But there is production routine complexity in this kind of microbial ecological agent, production cost in unfavorable factors such as transport and storage stage easy in inactivation, thus limits the propagation and employment of this type of microbial ecological agent higher than antibiotic, bacterial strain to a certain extent.
Summary of the invention
The object of this invention is to provide a kind of microbial ecological agent preventing and treating piglet diarrhea and preparation method thereof.This microbial ecological agent is that raw material is prepared from swine excrement, for preventing and treating piglet diarrhea, has significant effect, thus can reduce the antibiotic use amount of pig in control piglet diarrhea process.
For achieving the above object, the invention provides a kind of microbial ecological agent preventing and treating piglet diarrhea, described microbial ecological agent is made up of the feces bacterium liquid of pig and bacterial strain protective agent.Wherein, in every milliliter of microbial ecological agent, number of viable is 10
3~ 10
10individual.
Further, described pig is the fragrant pig in the Congjiang, Guizhou (a kind of few place of china product boar that diarrhoea occurs) of healthy growth.
Again further, the feces bacterium solution preparation method of described pig is: the fragrant swine excrement in the Congjiang, Guizhou collecting healthy growth, join in physiological saline solution and make suspension, filter suspension successively with the leakage of rustless steel tea and cell sieve (aperture is 0.224 millimeter), obtain the feces bacterium liquid of pig.Adopt viable bacteria counting method to the count plate in swine excrement bacterium liquid, and according to count results, the viable bacteria content in swine excrement bacterium liquid is adjusted to 10
3~ 10
10individual/milliliter.
Again further, the antibacterial at least containing one or more classes following in the feces bacterium liquid of described pig and fungus.These antibacterial classes comprise Bacteroidetes, Firmicutes, spirillum door, Proteobacteria, actinomycetes door, syntrophism bacterium door, wart germ door, wide ancient bacterium door, Fusobacterium door, cellulomonas door, Tenericutes, chlamydia door, deferrization bacillus door, fan's track bacterium door; Fungus class comprises Ascomycota, Basidiomycota, Zygomycota.
Further qualification finds, the antibacterial at least containing one or more genus classes following in the feces bacterium liquid of described pig and fungus.These antibacterials belong to that class comprises Bacteroides, Campylobacter, fusobacterium, Desulfovibrio, Lactobacillus, quiver bacillus gram genus, general Bordetella, Ruminococcus, Streptococcus, Succinivibrio, treponema; Fungi class comprises Alternaria, Eurotium, mycocandida, Cladosporium, Debaryomyces, Hansenula, Hypoxylon genus, Penicillium, Peniophora genus, Phoma, paecilomyces, Talaromyces, spore torulopsis, trichoderma, Trichosporon.
Again further, described bacterial strain protective agent to be mass fraction be 0.5% ~ 5% sodium bicarbonate solution.
Present invention also offers a kind of preparation method of preventing and treating the microbial ecological agent of piglet diarrhea, comprise the following steps:
1) collect the fragrant swine excrement in the Congjiang, Guizhou of healthy growth, join in physiological saline solution and make suspension, filter suspension successively with the leakage of rustless steel tea and cell sieve (aperture is 0.224 millimeter), obtain the feces bacterium liquid of pig.Adopt viable bacteria counting method to the count plate in swine excrement bacterium liquid, and according to count results, the viable bacteria content in swine excrement bacterium liquid is adjusted to 10
3~ 10
10individual/milliliter.
2) by step 1) in obtained swine excrement bacterium liquid and bacterial strain protective agent by volume (1:1 ~ 5:1) mix homogeneously, obtain microbial ecological agent.Wherein, in every milliliter of microbial ecological agent, number of viable is 10
3~ 10
10individual.
As transported for long-distance or long-term storage, in order to keep the activity of bacterium in swine excrement and swine excrement bacterium liquid, following two kinds of methods can be used:
1) collect the fragrant swine excrement in the Congjiang, Guizhou of healthy growth, be placed in liquid nitrogen cryopreservation.By 37 DEG C of water-bath quick-thawing swine excrements before using, then for the preparation of microbial ecological agent.
2) collect the fragrant swine excrement in the Congjiang, Guizhou of healthy growth, join in physiological saline solution and make suspension, filter suspension successively with the leakage of rustless steel tea and cell sieve (aperture is 0.224 millimeter), obtain the feces bacterium liquid of pig.Adopt viable bacteria counting method to the count plate in swine excrement bacterium liquid, and according to count results, the viable bacteria content in swine excrement bacterium liquid is adjusted to 10
4~ 10
11individual/milliliter.Then in swine excrement bacterium liquid, add glycerol obtain swine excrement bacterium liquid cryopreserving liquid (volume fraction of glycerol is 10%), be placed in liquid nitrogen cryopreservation.By the bacterium in 37 DEG C of water-bath rapid fluid resuscitation swine excrement bacterium liquid before using, then being diluted by the viable bacteria content in the swine excrement bacterium liquid after recovery is 10
3~ 10
10individual/milliliter, finally adds bacterial strain protective agent, mix homogeneously, makes microbial ecological agent.
During use, gavaged by microbial ecological agent in piglet stomach, gavaging dosage is 1 ~ 10 milliliter/time, and day takes once.
Microbial ecological agent of the present invention, compared with existing pig microbial ecological agent, has following advantage:
1) microbial ecological agent manufacturing process of the present invention is simple, and production cost is lower, has significant control piglet diarrhea effect, and does not affect the growth performance of piglet.
2) microbial ecological agent of the present invention contributes to the cycling and reutilization of swine excrement, realizes turning waste into wealth, and reduces swine excrement to the pollution of environment.
Detailed description of the invention
In order to explain the present invention better, illustrate main contents of the present invention further below in conjunction with specific embodiment, but content of the present invention is not only confined to following examples.
Embodiment 1
The preparation method of microbial ecological agent is:
1) collect healthy growth and the fragrant porkling swine excrement in the Congjiang, Guizhou of the 8th day after wean, be placed in liquid nitrogen cryopreservation.When preparing swine excrement bacterium liquid, by 37 DEG C of water-bath quick-thawing swine excrements, then join in physiological saline solution and make suspension.Filter suspension successively with the leakage of rustless steel tea and cell sieve (aperture is 0.224 millimeter), obtain the feces bacterium liquid of pig.Adopt viable bacteria counting method to the count plate in swine excrement bacterium liquid, and according to count results, the viable bacteria content in swine excrement bacterium liquid is adjusted to 10
4~ 10
11individual/milliliter, then adds glycerol and obtains swine excrement bacterium liquid cryopreserving liquid (volume fraction of glycerol is 10%), be placed in liquid nitrogen cryopreservation in swine excrement bacterium liquid.
Wherein, in the feces bacterium liquid of described pig at least containing following one or more belong to antibacterial and the fungus of classes.These antibacterials belong to that class comprises Bacteroides, Campylobacter, fusobacterium, Desulfovibrio, Lactobacillus, quiver bacillus gram genus, general Bordetella, Ruminococcus, Streptococcus, Succinivibrio, treponema; Fungi class comprises Alternaria, Eurotium, mycocandida, Cladosporium, Debaryomyces, Hansenula, Hypoxylon genus, Penicillium, Peniophora genus, Phoma, paecilomyces, Talaromyces, spore torulopsis, trichoderma, Trichosporon.
2) by the bacterium in 37 DEG C of water-bath rapid fluid resuscitation swine excrement bacterium liquid before using, then being diluted by the viable bacteria content in the swine excrement bacterium liquid after recovery is 10
3~ 10
10individual/milliliter.By swine excrement bacterium liquid and the bacterial strain protective agent after dilution by volume (1:1 ~ 5:1) mix homogeneously, obtain microbial ecological agent.Wherein, in every milliliter of microbial ecological agent, viable bacteria content is 10
3~ 10
10individual.
Piglet age in days the 10th, 12,14,16,18 days, gavaged by the microbial ecological agent of preparation in length × large two-way cross piglet stomach, gavaging dosage was 1 ~ 10 milliliter/time, and day takes once.With do not implement compared with length × large two-way cross piglet group that this microbial ecological agent gavages, this microbial ecological agent gavage group wean (21 age in days) afterwards the diarrhea rate in 1st ~ 11 day stage significantly reduce 60%, growth performance is without significant change.
Embodiment 2
The preparation method of microbial ecological agent is:
1) collect healthy growth and the fragrant porkling swine excrement in the Congjiang, Guizhou of the 8th day after wean, be placed in liquid nitrogen cryopreservation.When preparing swine excrement bacterium liquid, by 37 DEG C of water-bath quick-thawing swine excrements, then join in physiological saline solution and make suspension.Filter suspension successively with the leakage of rustless steel tea and cell sieve (aperture is 0.224 millimeter), obtain the feces bacterium liquid of pig.Adopt viable bacteria counting method to the count plate in swine excrement bacterium liquid, and according to count results, the viable bacteria content in swine excrement bacterium liquid is adjusted to 10
4~ 10
11individual/milliliter, then adds glycerol and obtains swine excrement bacterium liquid cryopreserving liquid (volume fraction of glycerol is 10%), be placed in liquid nitrogen cryopreservation in swine excrement bacterium liquid.
Wherein, in the feces bacterium liquid of described pig at least containing following one or more belong to antibacterial and the fungus of classes.These antibacterials belong to that class comprises Bacteroides, Campylobacter, fusobacterium, Desulfovibrio, Lactobacillus, quiver bacillus gram genus, general Bordetella, Ruminococcus, Streptococcus, Succinivibrio, treponema; Fungi class comprises Alternaria, Eurotium, mycocandida, Cladosporium, Debaryomyces, Hansenula, Hypoxylon genus, Penicillium, Peniophora genus, Phoma, paecilomyces, Talaromyces, spore torulopsis, trichoderma, Trichosporon.
2) by the bacterium in 37 DEG C of water-bath rapid fluid resuscitation swine excrement bacterium liquid before using, then being diluted by the viable bacteria content in the swine excrement bacterium liquid after recovery is 10
3~ 10
10individual/milliliter.By swine excrement bacterium liquid and the bacterial strain protective agent after dilution by volume (1:1 ~ 5:1) mix homogeneously, obtain microbial ecological agent.Wherein, in every milliliter of microbial ecological agent, viable bacteria content is 10
3~ 10
10individual.
Gavaged respectively by the microbial ecological agent of preparation and occur in cacatory piglet stomach to 15 wean, gavaging dosage is 1 ~ 10 milliliter/time, and day takes once.Next day, the diarrhea rate of piglet reduces 50%.3rd, the symptom of diarrhea of all diarrhoea piglets disappeared substantially.
Other unspecified part is prior art.Although above-described embodiment is to invention has been detailed description; but it is only the present invention's part embodiment; instead of whole embodiment, people can also obtain other embodiments according to the present embodiment under without creative prerequisite, and these embodiments all belong to scope.
Claims (5)
1. prevent and treat a microbial ecological agent for piglet diarrhea, it is characterized in that: described microbial ecological agent is made up of the feces bacterium liquid of pig and bacterial strain protective agent; Wherein, in every milliliter of microbial ecological agent, viable bacteria content is 10
3~ 10
10individual.
2. prevent and treat the microbial ecological agent of piglet diarrhea according to claim 1, it is characterized in that: described pig is the fragrant pig in the Congjiang, Guizhou of healthy growth.
3. according to claim 1 or 2, prevent and treat the microbial ecological agent of piglet diarrhea, it is characterized in that: the feces bacterium solution preparation method of described pig is: the fragrant swine excrement in the Congjiang, Guizhou collecting healthy growth, join in physiological saline solution and make suspension, suspension is filtered successively with the leakage of rustless steel tea and cell sieve, obtain the feces bacterium liquid of pig, to the count plate in swine excrement bacterium liquid, and according to count results, the viable bacteria content in swine excrement bacterium liquid is adjusted to 10
3~ 10
10individual/milliliter.
4. according to claim 1 or 2, prevent and treat the microbial ecological agent of piglet diarrhea, it is characterized in that: described bacterial strain protective agent to be mass fraction be 0.5% ~ 5% sodium bicarbonate solution.
5. prevent and treat a microbial ecological agent preparation method for piglet diarrhea described in claim 1, it is characterized in that: comprise the following steps:
1) the fragrant swine excrement in the Congjiang, Guizhou of healthy growth is collected, join in physiological saline solution and make suspension, suspension is filtered successively with the leakage of rustless steel tea and cell sieve, obtain the feces bacterium liquid of pig, to the count plate in swine excrement bacterium liquid, and according to count results, the viable bacteria content in swine excrement bacterium liquid is adjusted to 10
3~ 10
10individual/milliliter;
2) by step 1) in obtained swine excrement bacterium liquid and bacterial strain protective agent by volume 1 ~ 5 ︰ 1 mix homogeneously, obtain microbial ecological agent; Wherein, in every milliliter of microbial ecological agent, viable bacteria content is 10
3~ 10
10individual.
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| CN201510740876.4A CN105343125A (en) | 2015-11-03 | 2015-11-03 | Microbial ecological agent for preventing and treating piglet diarrhea and preparation method of microbial ecological agent |
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| CN105343125A true CN105343125A (en) | 2016-02-24 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106511380A (en) * | 2016-10-25 | 2017-03-22 | 重庆市畜牧科学院 | Coprophilous fungus composition and preparation method and purpose thereof |
| CN107050064A (en) * | 2017-03-01 | 2017-08-18 | 华中农业大学 | Prevent and treat probiotics preparation of grice diarrhoea and its preparation method and application |
| WO2021134740A1 (en) * | 2020-01-03 | 2021-07-08 | 四川农业大学 | Tibetan pig fecal microbiota capsule for relieving diarrhea in weaned piglet, and preparation method therefor |
Citations (1)
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|---|---|---|---|---|
| CN104688780A (en) * | 2015-03-31 | 2015-06-10 | 徐高骁 | Method for preventing and treating piglet diarrhea based on caprophyl implantation technique |
-
2015
- 2015-11-03 CN CN201510740876.4A patent/CN105343125A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104688780A (en) * | 2015-03-31 | 2015-06-10 | 徐高骁 | Method for preventing and treating piglet diarrhea based on caprophyl implantation technique |
Non-Patent Citations (1)
| Title |
|---|
| 王晓波等: "仔猪腹泻的原因及防治", 《河南畜牧兽医》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106511380A (en) * | 2016-10-25 | 2017-03-22 | 重庆市畜牧科学院 | Coprophilous fungus composition and preparation method and purpose thereof |
| CN107050064A (en) * | 2017-03-01 | 2017-08-18 | 华中农业大学 | Prevent and treat probiotics preparation of grice diarrhoea and its preparation method and application |
| WO2021134740A1 (en) * | 2020-01-03 | 2021-07-08 | 四川农业大学 | Tibetan pig fecal microbiota capsule for relieving diarrhea in weaned piglet, and preparation method therefor |
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Application publication date: 20160224 |