CN105309307A - Butterfly orchid culture medium and culture method - Google Patents
Butterfly orchid culture medium and culture method Download PDFInfo
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- CN105309307A CN105309307A CN201510662201.2A CN201510662201A CN105309307A CN 105309307 A CN105309307 A CN 105309307A CN 201510662201 A CN201510662201 A CN 201510662201A CN 105309307 A CN105309307 A CN 105309307A
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- culture medium
- moth orchid
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- butterfly orchid
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Abstract
The invention discloses a butterfly orchid culture medium and culture method. An MS culture medium serves as a basic culture medium, and the culture medium is prepared from 15-20 g/L of cane sugar, 5-8 g/L of agar powder, 30-50 mg/L of gibberellin GA, 50-80 ml/L of silk reeling waste water and 20-30 g/L of edible fungi waste residues. The culture method comprises the steps of disinfecting pedicel axillary buds of butterfly orchid, then incising the pedicel axillary buds, then inoculating the pedicel axillary buds into the butterfly orchid culture medium, controlling a culture room at the constant temperature, controlling the temperature within the range from 20 DEG C to 25 DEG C and the illumination intensity within the range from 1500 Lux to 2000 Lux, conducting culture for 20-30 days, and obtaining butterfly orchid seedlings; inoculating the butterfly orchid seedlings into another culture medium, controlling a culture room at the constant temperature, controlling the temperature within the range from 25 DEG C to 28 DEG C and the illumination intensity within the range from 1500 Lux to 2000 Lux, and conducting culture for 30-40 days; leaving the butterfly orchid seedlings in the natural environment for 5-7 days, explanting the butterfly orchid seedlings out of the culture medium, and conducting potted planting. By means of the culture medium, the survival rate of the butterfly orchid pedicel can be effectively raised, harm to explants caused by bactericide is reduced, germination of the axillary buds is promoted, and germination time is shortened.
Description
Technical field
The present invention relates to field of plant tissue culture technique, particularly relate to a kind of moth orchid culture medium and use the method for this medium culture Moth orchid.
Background technology
Moth orchid (Phalaenopsis) is the orchid family Phalaenopsis perennial herb flowers, originate in subtropical and tropical zones, its flower likeness in form butterfly, pattern is gorgeous, florescence is lasting, having high ornamental value and economic worth, is one of potted flower the most fast-selling on the outer flowers market of Now Domestic.
Because Moth orchid is single stem aerial orchid, plant seldom grows side shoot, is difficult to adopt conventional plant division mode to carry out vegetative propagation.Its seed is not also containing endosperm or its hetero-organization, and germination rate is extremely low under field conditions (factors), and seeding method therefore cannot be utilized to carry out sexual propagation.And adopt method for tissue culture to carry out unique channel that sapling multiplication is current Moth orchid large-scale production.The approach of Plant Tissue Culture of Phalaenopsis hybrid mainly utilizes aseptic seeding and utilizes various types of explant induction protocorms, and then induced meristem seedling realizes numerous soon, directly need not break up Multiple Buds by evoked callus.
Summary of the invention
The object of the present invention is to provide a kind of moth orchid culture medium, this medium uses Moth orchid pedicel axillary buds to cultivate, this medium can improve Moth orchid bennet survival rate, silk reeling wastewater and edible mushroom waste residue etc. is used to not only reduce production cost and recycle waste material, environmental protection and energy saving.The technical scheme used for realizing the object of the invention is:
A kind of moth orchid culture medium, medium based on MS medium, also comprises following raw material: sucrose 15-20g/L, agar powder 5-8g/L, gibberellin GA30-50mg/L, silk reeling wastewater 50-80ml/L and edible mushroom waste residue 20-30g/L.
First edible mushroom waste residue is fermented under higher temperature conditions, with the simple organic matter recovery by moldy metamorphism easy in material, ensure the stable chemical performance of matrix, concrete operations are as follows: amassed by waste edible fungus slag muck and cover Polypropylence Sheet, make stack temperature rise to 45 ~ 65 DEG C, then carry out oxygen supply by turning or ventilation, carry out moisturizing to guarantee the temperature be suitable in good time, be rich in nitrogenous fertilizer and phosphate fertilizer in edible mushroom waste residue, after a period of time, temperature drops to normal temperature.
Be rich in a large amount of ammonia nitrogens, organic nitrogen and pupa albumen in silk reeling wastewater, directly discharge in silk processing factory, river course eutrophication not only can be caused to produce and pollute, and be the significant wastage to resource.
Described medium also comprises tea waste residue 5-10g/L.
Described sucrose 18-20g/L, agar powder 5-7g/L, gibberellin GA40-50mg/L, silk reeling wastewater 60-80ml/L and edible mushroom waste residue 25-30g/L.
Described tea waste residue be black tea, green tea, white tea, yellow tea or Oolong tea brewed after, residual tealeaves.
Described tea waste residue is pulverized in advance, is ground into the particle that particle diameter is 1-2mm.
A cultural method for Moth orchid, concrete cultural method is
(1) after the pedicel axillary buds of Moth orchid being carried out disinfection, to pedicel axillary buds otch, inoculate in moth orchid culture medium according to claim 1, thermostatic control culturing room, temperature controls at 20-25 DEG C, and intensity of illumination is 1500-2000Lux, cultivate 20-30 days, obtain Moth orchid seedling;
(2) by step 1) the Moth orchid seedling that obtains is inoculated in medium according to claim 2, thermostatic control culturing room, temperature control 25-28 DEG C, intensity of illumination is 1500-2000Lux, cultivates 30-40 days;
(3) the Moth orchid seedling that step (2) obtains to be placed under natural environment after 5-7 days, to shift out from medium, above potted plantly to plant.
Useful object of the present invention for: make full use of the nitrogen, P elements and the albumen that are rich in silk reeling wastewater, use the nitrogenous fertilizer of edible mushroom waste residue and phosphate fertilizer etc., not only reduce production cost and waste material is recycled, environmental protection and energy saving.Adopt sucrose as carbon source in inducing culture, under the prerequisite that bennet survival rate and germination rate are not affected, can effectively reduce costs.In medium of the present invention, gibberellin GA soaks bennet, effectively can improve the survival rate of Moth orchid bennet, reduces bactericide to the injury of explant, promotes axillary bud sprouting, shorten sprout time.
Embodiment
Embodiment 1
Medium based on MS medium, containing sucrose 15-20g, agar powder 5-8g, gibberellin GA30-50mg, silk reeling wastewater 50-80ml and edible mushroom waste residue 20-30g in often liter of medium, concrete preparation method is:
1) first edible mushroom waste residue is fermented, waste edible fungus slag muck is amassed and covers Polypropylence Sheet, make stack temperature rise to 45 ~ 65 DEG C, then carry out oxygen supply by turning or ventilation, carry out moisturizing to guarantee the temperature be suitable in good time.
2) weigh agar powder by above-mentioned raw materials proportioning to add in the pot filling 1 liter of pure water and boil, weigh enough sucrose to add in pot and to make it melt, get 0.1 liter of 10 times of MS mother liquor and gibberellin GA again, the edible mushroom waste residue of silk reeling wastewater and fermentation adds in pot, constant volume, to 1 liter, obtains moth orchid culture medium.
Embodiment 2
Medium based on MS medium, containing sucrose 18-20g, agar powder 5-7g, gibberellin GA40-50mg, silk reeling wastewater 60-80ml and edible mushroom waste residue 25-30g in often liter of medium, concrete preparation method is:
1) first edible mushroom waste residue is fermented, waste edible fungus slag muck is amassed and covers Polypropylence Sheet, make stack temperature rise to 45 ~ 65 DEG C, then carry out oxygen supply by turning or ventilation, carry out moisturizing to guarantee the temperature be suitable in good time.
2) weigh agar powder by above-mentioned raw materials proportioning to add in the pot filling 1 liter of pure water and boil, weigh enough sucrose to add in pot and to make it melt, get 0.1 liter of 10 times of MS mother liquor and gibberellin GA again, the edible mushroom waste residue of silk reeling wastewater and fermentation adds in pot, constant volume, to 1 liter, obtains moth orchid culture medium.
Embodiment 3
Medium based on MS medium, containing sucrose 15-18g, agar powder 6-8g, gibberellin GA35-45mg, silk reeling wastewater 55-75ml, tea waste residue 5-10g/L and edible mushroom waste residue 22-28g in often liter of medium, concrete preparation method is:
1) first edible mushroom waste residue is fermented, waste edible fungus slag muck is amassed and covers Polypropylence Sheet, make stack temperature rise to 45 ~ 65 DEG C, then carry out oxygen supply by turning or ventilation, carry out moisturizing to guarantee the temperature be suitable in good time; Tea waste residue is ground into the particle that particle diameter is 1-2mm.
2) weigh agar powder by above-mentioned raw materials proportioning to add in the pot filling 1 liter of pure water and boil, weigh enough sucrose to add in pot and to make it melt, get 0.1 liter of 10 times of MS mother liquor and gibberellin GA, silk reeling wastewater, tea waste residue and fermentation edible mushroom waste residue add in pot, constant volume, to 1 liter, obtains moth orchid culture medium.
Embodiment 4
(1) after the pedicel axillary buds of Moth orchid being carried out disinfection, to pedicel axillary buds otch, inoculate in the moth orchid culture medium described in embodiment 1, thermostatic control culturing room, temperature controls at 20-25 DEG C, and intensity of illumination is 1500-2000Lux, cultivate 20-30 days, obtain Moth orchid seedling;
(2) by step 1) the Moth orchid seedling that obtains is inoculated in the medium described in embodiment 3, thermostatic control culturing room, temperature control 25-28 DEG C, intensity of illumination is 1500-2000Lux, cultivates 30-40 days;
(3) the Moth orchid seedling that step (2) obtains to be placed under natural environment after 5-7 days, to shift out from medium, above potted plantly to plant.
Claims (6)
1. a moth orchid culture medium, medium based on MS medium, is characterized in that, also comprises following raw material: sucrose 15-20g/L, agar powder 5-8g/L, gibberellin GA30-50mg/L, silk reeling wastewater 50-80ml/L and edible mushroom waste residue 20-30g/L.
2. moth orchid culture medium according to claim 1, is characterized in that, described medium also comprises tea waste residue 5-10g/L.
3. moth orchid culture medium according to claim 1, is characterized in that, described sucrose 18-20g/L, agar powder 5-7g/L, gibberellin GA40-50mg/L, silk reeling wastewater 60-80ml/L and edible mushroom waste residue 25-30g/L.
4. moth orchid culture medium according to claim 2, is characterized in that, described tea waste residue be black tea, green tea, white tea, yellow tea or Oolong tea brewed after, residual tealeaves.
5. moth orchid culture medium according to claim 2, is characterized in that, described tea waste residue is pulverized in advance, is ground into the particle that particle diameter is 1-2mm.
6. the cultural method of Moth orchid according to claim 1, is characterized in that, concrete cultural method is
(1) after the pedicel axillary buds of Moth orchid being carried out disinfection, to pedicel axillary buds otch, inoculate in moth orchid culture medium according to claim 1, thermostatic control culturing room, temperature controls at 20-25 DEG C, and intensity of illumination is 1500-2000Lux, cultivate 20-30 days, obtain Moth orchid seedling;
(2) by step 1) the Moth orchid seedling that obtains is inoculated in medium according to claim 2, thermostatic control culturing room, temperature control 25-28 DEG C, intensity of illumination is 1500-2000Lux, cultivates 30-40 days;
(3) the Moth orchid seedling that step (2) obtains to be placed under natural environment after 5-7 days, to shift out from medium, above potted plantly to plant.
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| CN201510662201.2A CN105309307A (en) | 2015-10-15 | 2015-10-15 | Butterfly orchid culture medium and culture method |
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| CN201510662201.2A CN105309307A (en) | 2015-10-15 | 2015-10-15 | Butterfly orchid culture medium and culture method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109662010A (en) * | 2019-02-14 | 2019-04-23 | 芜湖东源新农村开发股份有限公司 | It is a kind of to prevent iris rotten culture substrate and preparation method thereof |
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