CN105284450A - Method for quickly inducing rice blast via box type seedling nursing - Google Patents
Method for quickly inducing rice blast via box type seedling nursing Download PDFInfo
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- CN105284450A CN105284450A CN201510603420.3A CN201510603420A CN105284450A CN 105284450 A CN105284450 A CN 105284450A CN 201510603420 A CN201510603420 A CN 201510603420A CN 105284450 A CN105284450 A CN 105284450A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses a method for quickly inducing rice blast via box type seedling nursing. Paddy rice seedlings are cultured in sealed box type small environment; rice blast bacteria is inoculated during a fine rice-grain sprout stage and an inoculation morbidity result can be achieved in short period. The method comprises the following steps of (1) preparing matching nutrition solution, (2) filling seedling nursing soil, (3) broadcasting, (4) nursing paddy rice in the box type small environment, (5) preparing inoculant spore liquid, (6) inoculating bacteria and (7) managing nursing after inoculation. The method for quickly inducing rice blast via box type seedling nursing is advantageous in that the whole method requires less time and only eight days are required from broadcasting to typical morbidity; conditions having a close relationship with rice blast morbidity such as temperature, humidity and water and fertilizer are highly uniformed and can be easily controlled; the method can be simply operated, has a simple technology on the whole and can achieve technology standardization.
Description
Technical field
The present invention relates to plant pathology to learn a skill and microbiological technique.Specifically the method for rice blast is fast brought out in a kind of boxlike nursery.
Background technology
Rice blast is the great disease of China's Rice Production, and year of being very popular once caused rice grain to lose 15,000,000,000 kilograms to China.Although quite deep to the understanding of rice blast at present, the harm continuing to control anti-rice blast safely and efficiently realized, still need large quantifier elimination to drop into.And many researchs of rice blast all be unable to do without this basic means of manual-induced disease, even the research in molecule field, as germ pathogenic related gene problem, or host's disease-resistant related gene problem, often all need the result of manual-induced disease to answer.So far, about the technology of the manual-induced disease of rice blast is quite ripe, these bring out disease technology and sum up, and mainly contain paddy rice Adult plant inoculation morbidity and the large class of Seedling Inoculation morbidity method two.Usual Adult plant brings out disease can obtain the result comparatively suiting and produce reality, thus applies more in the comparation and assessment of Rice Resistance characteristic of disease and some production test, but brings out in the method system of disease at Adult plant, there is time and effort consuming; Quite take up space place; Environmental condition easily affects the outstanding disadvantages such as result of the test; Thus its application in many experiment works is limited.Usually, bring out the method for disease seedling stage, relatively little than Adult plant of the rice seedling of use, compared with bringing out disease, there is change test holding time and aspect, space greatly with Adult plant.But, bringing out disease method the seedling stage widely applied at present is all 3 ~ 5 leaf after dates at rice seedling, the seedling leaves launched completely is utilized to inoculate, in this method system, more than 2 weeks consuming time of nursery, inoculation is observed more than 1 week consuming time, test period will cross over the duration more than 3 weeks usually, this requires to some the research work obtaining result in a short time, as molecule plants the pathogenic mutation body qualification of disease research, disease-resistant gene is expressed, and the germ-host of infection processs does information transmission etc. mutually, still has long suspicion consuming time; And, for obtaining healthy and strong rice seedling, need to provide sufficient sunlight and extra-nutrition, the place, space that whole method system still needs to use greenhouse etc. larger; The local condition such as humidity, temperature, rich water of duration of test controls, and also there is larger technical difficulty, or quite by the restriction etc. of equipment condition.
Summary of the invention
The object of this invention is to provide a kind of method that rice blast is fast brought out in boxlike nursery.
The technical scheme that the present invention solves the problems of the technologies described above is as follows:
A method for rice blast is fast brought out in boxlike nursery, is to cultivate rice seedling under the boxlike subenvironment closed, and obtain morbidity result fast at needle-like young shoot stage inoculation Pyricularia oryzae, implementation and operation step is as follows:
1. prepare supporting nutrient solution: prepare supporting nutrient solution in proportion, this nutrient solution prescription is: potassium nitrate 1.0g, potassium dihydrogen phosphate 1.0g, water 1000.0ml.
2. load seedling soil: take out clean seedling culture basin, primary side coil, large square plate and moisture preserving cover, seedling culture basin is put into primary side coil, then gets the smooth all basin caves of filling seedling culture basin respectively of the mold after pulverizing; The nutrient solution getting step 1 outfit injects in primary side coil, after whole soil of nutrient solution permeable water-raised seedling basin, keeps there is thin layer nutrient solution in primary side coil.
3. sow: condition is by rice paddy seed seed soaking and vernalization routinely, obtains the germination seed that shows money or valuables one carries unintentionally, the rice paddy seed of the sprouting that showed money or valuables one carries unintentionally with pincet gripping, broadcasts in the moistening soil of step 2 seedling culture basin, every basin bunch planting 1 seed respectively.
4. in boxlike microhabitat, cultivate paddy rice: after treating that step 3 is sowed, primary side coil and the material that contains thereof are placed in large square plate together; get moisture preserving cover and primary side coil is covered in large square plate; in large square plate, inject thin layer clear water, make to be in closed state in moisture preserving cover, form the seedling microhabitat of a boxlike; then this complete set of seedling implement is forwarded on the desktop of the indoor nearly window of routine experimentation; under room temperature is 28 DEG C of conditions, cultivate paddy rice, until the first complete leaf growth is exposed, form needle-like young shoot.
5. prepare inoculum spore liquid: cultivate the conidium of preparing Pyricularia oryzae according to a conventional method, suspended by spore wash-out with clear water, being made into spore concentration is 1 × 10
6the spore liquid of individual/ml, as inoculum spore liquid.
6. inoculate germ: inoculum spore liquid step 5 prepared loads sprayer, take out the needle-like paddy rice young shoot material that step 4 prepares, open the moisture preserving cover covered on outside young shoot, inoculum spore liquid facing to young shoot even spraying, till needle-like young shoot surface is covered with one deck droplet, then again cover back moisture preserving cover.
7. cultivating and managing after inoculation: by postvaccinal for step 6 young shoot, again go back to step 4 nursery part, keeps the temperature condition of original nursery to continue to cultivate; Inoculate after 5 days, immature rice seedling occurs the downright bad scab of rice blast clearly.
Advantage of the present invention is:
1) overall procedure all times is short, and from being seeded into, typical case's morbidity is general only needs 8 days.
2) close with the onset relation of rice blast condition, as temperature, humidity, liquid manure etc. all can be highly consistent, and easily controls.
3) implementation and operation is simple and easy, and general technical is simple, the standardization of easy actualizing technology method.
Accompanying drawing explanation
Fig. 1 is the present invention's utensil schematic diagram used.
In figure, seedling culture basin is placed in primary side coil, and primary side coil is placed in large square plate, and material cover in primary side coil and dish thereof is enclosed in large square plate by transparent lucite moisture preserving cover, forms the little space of a boxlike in cover.
Fig. 2 is the needle-like young shoot that the present invention sows that after 3 days, rice paddy seed growth is formed.
Fig. 3 is the downright bad scab that paddy rice young shoot of the present invention inoculation Pyricularia oryzae occurs for 5 days afterwards.
Embodiment
Below in conjunction with accompanying drawing and embodiment, the invention will be further described.
A method for rice blast is fast brought out in boxlike nursery, is to cultivate rice seedling under the boxlike subenvironment closed, and obtain morbidity result fast at needle-like young shoot stage inoculation Pyricularia oryzae, implementation and operation step is as follows:
1. prepare supporting nutrient solution: prepare supporting nutrient solution in proportion, this nutrient solution prescription is: potassium nitrate 1.0g, potassium dihydrogen phosphate 1.0g, water 1000.0ml.
2. load seedling soil: take out clean seedling culture basin, primary side coil, large square plate and moisture preserving cover, seedling culture basin is put into primary side coil, then gets the smooth all basin caves of filling seedling culture basin respectively of the mold after pulverizing; The nutrient solution getting step 1 outfit injects in primary side coil, after whole soil of nutrient solution permeable water-raised seedling basin, keeps there is thin layer nutrient solution in primary side coil.
3. sow: condition is by rice paddy seed seed soaking and vernalization routinely, obtains the germination seed that shows money or valuables one carries unintentionally, the rice paddy seed of the sprouting that showed money or valuables one carries unintentionally with pincet gripping, broadcasts in the moistening soil of step 2 seedling culture basin, every basin bunch planting 1 seed respectively.
4. in boxlike microhabitat, cultivate paddy rice: after step 3 is sowed, primary side coil and the material that contains thereof are placed in large square plate together; get moisture preserving cover and primary side coil is covered in large square plate; in large square plate, inject thin layer clear water, make to be in closed state in moisture preserving cover, form the seedling microhabitat of a boxlike; then this complete set of seedling implement is forwarded on the desktop of the indoor nearly window of routine experimentation; under room temperature is 28 DEG C of conditions, cultivate paddy rice, until the first complete leaf growth is exposed, form needle-like young shoot.
5. prepare inoculum spore liquid: cultivate the conidium of preparing Pyricularia oryzae according to a conventional method, suspended by spore wash-out with clear water, being made into spore concentration is 1 × 10
6the spore liquid of individual/ml, as inoculum spore liquid.
6. inoculate germ: inoculum spore liquid step 5 prepared loads sprayer, take out the needle-like paddy rice young shoot material that step 4 prepares, open the moisture preserving cover covered on outside young shoot, inoculum spore liquid facing to young shoot even spraying, till needle-like young shoot surface is covered with one deck droplet, then again cover back moisture preserving cover.
7. cultivating and managing after inoculation: by postvaccinal for step 6 young shoot, again go back to step 4 nursery part, keeps the temperature condition of original nursery to continue to cultivate; Inoculate after 5 days, immature rice seedling occurs the downright bad scab of rice blast clearly.
The present invention adopts following technology to solve conventional technology disadvantage of bringing out rice blast seedling stage: 1) at the needle-like young shoot stage inoculation Pyricularia oryzae of rice seedling, thus can significantly shorten the test period of bringing out rice blast; 2) by seedling cultivation of rice and inoculation morbidity technical system, design in a relatively independent and closed little space of boxlike, thus can simplify technical equipment and condition to large extent.
The primary side coil that the present invention uses and the true square plate appliances tested room routine and use of large square plate, the common seedling culture basin that seedling culture basin adopts Rice Production to use, being cut into by seedling culture basin with cutting knife to be applicable to inserting in common primary side coil at once, and moisture preserving cover is organic moisture cover, as shown in Figure 1.Seedling medium adopts common mold, dries for subsequent use after being pulverized by earth materials; The object of supporting nutrient solution improves the effect improving and bring out rice blast.
Before sowing, in the usual way rice paddy seed is soaked seed at temperature is 30 DEG C 1 day and vernalization 1 day, obtain the seed showing money or valuables one carries unintentionally and sprout; When preparing sowing, first seedling culture basin to be placed in primary side coil and smoothly to fill soil, then supporting nutrient solution is injected in primary side coil, nutrient solution infiltrates the soil in basin by the aperture bottom seedling culture basin, filling nutrient solution until the whole soil of seedling culture basin is fully moistening, and keeps thin layer nutrient solution in primary side coil; During sowing, the rice paddy seed sprouted with pincet gripping, is pressed in the soil of seedling culture basin gently, 1 basin bunch planting, 1 seed.
After sowing, material in primary side coil and dish is forwarded in a large square plate, injects thin layer clear water toward in large square plate, then get moisture preserving cover material in primary side coil and dish thereof is all covered in large square plate, form rice paddy seed and be in a closed boxlike microhabitat; Then complete appliance and rice material contained above thereof are placed on the experiment desktop near the indoor window of routine experimentation and cultivate, indoor temperature is adjusted to 28 DEG C, and illumination condition maintains daily scattered light, without the need to increasing artificial light.
This technical system, usual rice growing grew coleoptile after 1 day, can grow imperfect leaf after 2 days, can grow the first complete leaf after 3 days.The period that the present invention designs artificial access germ be the first complete leaf stretch out but blade also do not launch before the needle-like young shoot phase, as shown in Figure 2, obviously, sow and can carry out germ inoculation after 3 days, thus nursery required time can significantly shorten than conventional seedbed system required time.
Only moisture preserving cover need be opened during inoculation, by spore liquid facing to the spraying of paddy rice young shoot, until young shoot surface is covered with spore droplet, inoculates and complete moisture preserving cover is in statu quo covered back material, and continue under complete appliance and material being relayed back the condition of original nursery to cultivate, until obtain morbidity result.
Observe from being seeded into result, except inoculation action need is opened except moisture preserving cover, midway is without the need to opening moisture preserving cover ventilation and keeping the skin wet, and after the growth of young shoot and inoculation, young shoot and germ are caused a disease Interaction, is all in a relatively independent and closed boxlike microhabitat.Visible, the condition close with rice blast inducible relationship, as soil nutrient, cultivation temperature, subenvironment humidity etc. all can keep highly consistent, and be very easy to control, thus comparatively easily realizes standardization in technical controlling.
The present invention's design bring out rice blast technical system, usually inoculation is after 3 days, and inoculation position starts to manifest scab point, 5 days visible larger downright bad scabs afterwards, as shown in Figure 3.
Embodiment 1
A method for rice blast is fast brought out in boxlike nursery, fragrant excellent 7633 rice seedlings should bring out rice blast, operate as follows with Pyricularia oryzae bacterial strain Mg2013-1 at rice varieties:
1. prepare supporting nutrient solution
Prepare supporting nutrient solution in proportion, this nutrient solution prescription is: potassium nitrate 1.0g, potassium dihydrogen phosphate 1.0g, water 1000.0ml.
2. load seedling soil
Take out clean seedling culture basin, primary side coil, large square plate and moisture preserving cover, seedling culture basin is put into primary side coil, then gets the smooth all basin caves of filling seedling culture basin respectively of the mold after pulverizing; The nutrient solution getting step 1 outfit injects in primary side coil, after whole soil of nutrient solution permeable water-raised seedling basin, keeps there is thin layer nutrient solution in primary side coil.
3. sow
First should fragrant excellent 7633 seed soaking 1 day and vernalization 1 day by rice varieties under 30 DEG C of temperature condition, obtain the germination seed that shows money or valuables one carries unintentionally.To show money or valuables one carries unintentionally the rice paddy seed sprouted with pincet gripping, broadcast and be embedded in step 2 and load in the moistening soil got ready, every basin bunch planting 1 seed.
4. in boxlike microhabitat, cultivate paddy rice
After step 3 is sowed, primary side coil and the material that contains thereof are placed in large square plate together; get moisture preserving cover and primary side coil is covered in large square plate; in large square plate, inject thin layer clear water, make to be in closed state in moisture preserving cover, form the seedling microhabitat of a boxlike; then this complete set of seedling implement is forwarded on the desktop of the indoor nearly window of routine experimentation; under room temperature is 28 DEG C of conditions, cultivate paddy rice, until the first complete leaf growth is exposed, form needle-like young shoot.
5. prepare inoculum spore liquid
Cultivate the conidium of preparing bacterial strain Mg2013-1 according to a conventional method with oat medium (oat medium component ratio: oat 167g, agar 20g, water 1000ml), being made into spore concentration is 1 × 10
6the spore liquid of individual/ml, as inoculum spore liquid.
6. inoculate germ
Inoculum spore liquid step 5 prepared loads sprayer, take out the needle-like paddy rice young shoot material that step 4 prepares, open the moisture preserving cover covered on outside young shoot, inoculum spore liquid facing to young shoot even spraying, till needle-like young shoot surface is covered with one deck droplet, then again cover back moisture preserving cover.
7. cultivating and managing after inoculation
By postvaccinal for step 6 young shoot, again go back to original nursery part, keep the temperature condition of step 4 nursery to continue to cultivate; Inoculate after 5 days, with shown in Fig. 3, immature rice seedling occurs the downright bad scab of rice blast clearly.
Embodiment 2
The method of rice blast is fast brought out in a kind of boxlike nursery, on special excellent 838 rice seedlings of rice varieties, rice blast is brought out with Pyricularia oryzae bacterial strain Mg2013-1, operate to step 7 by the step 1 of embodiment 1, the rice varieties that just step 3 is used is special excellent 838, result is in inoculation after 5 days, also with shown in Fig. 3, excellent 838 rice seedlings of spy occur the downright bad scab of rice blast clearly.
Claims (1)
1. a method for rice blast is fast brought out in boxlike nursery, it is characterized in that, under the boxlike subenvironment closed, cultivate rice seedling, and obtain morbidity result fast at needle-like young shoot stage inoculation Pyricularia oryzae, implementation and operation step is as follows:
1) prepare supporting nutrient solution: prepare supporting nutrient solution in proportion, this nutrient solution prescription is: potassium nitrate 1.0g, potassium dihydrogen phosphate 1.0g, water 1000.0ml;
2) seedling soil is loaded: take out clean seedling culture basin, primary side coil, large square plate and moisture preserving cover, seedling culture basin is put into primary side coil, then gets the smooth all basin caves of filling seedling culture basin respectively of the mold after pulverizing; Get step 1) nutrient solution that is equipped with injects in primary side coil, after whole soil of nutrient solution permeable water-raised seedling basin, keeps there is thin layer nutrient solution in primary side coil;
3) sow: condition is by rice paddy seed seed soaking and vernalization routinely, obtains and to show money or valuables one carries unintentionally germination seed, to have showed money or valuables one carries unintentionally the rice paddy seed sprouted with pincet gripping, broadcast respectively in step 2) in the moistening soil of seedling culture basin, every basin bunch planting 1 seed;
4) in boxlike microhabitat, cultivate paddy rice: treat step 3) sowing after, primary side coil and the material that contains thereof are placed in large square plate together; get moisture preserving cover and primary side coil is covered in large square plate; in large square plate, inject thin layer clear water; make to be in closed state in moisture preserving cover; form the seedling microhabitat of a boxlike; then this complete set of seedling implement is forwarded on the desktop of the indoor nearly window of routine experimentation; under room temperature is 28 DEG C of conditions, cultivate paddy rice; until the first complete leaf growth is exposed, form needle-like young shoot;
5) prepare inoculum spore liquid: cultivate the conidium of preparing Pyricularia oryzae according to a conventional method, suspended by spore wash-out with clear water, being made into spore concentration is 1 × 10
6the spore liquid of individual/ml, as inoculum spore liquid;
6) germ is inoculated: by step 5) the inoculum spore liquid for preparing loads sprayer, take out step 4) the needle-like paddy rice young shoot material for preparing, open the moisture preserving cover covered on outside young shoot, inoculum spore liquid facing to young shoot even spraying, till needle-like young shoot surface is covered with one deck droplet, then again cover back moisture preserving cover;
7) cultivating and managing after inoculation: by step 6) postvaccinal young shoot, again go back to step 4) nursery part, keep the temperature condition of original nursery to continue to cultivate; Inoculate after 5 days, immature rice seedling occurs the downright bad scab of rice blast clearly.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105917989A (en) * | 2016-05-24 | 2016-09-07 | 广西大学 | Simple method for determining control effect of rice blast fungicide |
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| CN105145303A (en) * | 2015-09-10 | 2015-12-16 | 广西大学 | Method for rapidly inducing rice blast of pure sand seedlings |
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| CN1824791A (en) * | 2005-12-27 | 2006-08-30 | 云南农业大学 | Fast identification process of plant pathogenic bacterium pathogenicity |
| CN101348825A (en) * | 2008-09-08 | 2009-01-21 | 江苏省农业科学院 | Wheat sharp eyespot resistance seedling identification method |
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