CN103229674A - Method for simply and rapidly identifying resistance of pyricularia grisea - Google Patents
Method for simply and rapidly identifying resistance of pyricularia grisea Download PDFInfo
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- CN103229674A CN103229674A CN2013101891314A CN201310189131A CN103229674A CN 103229674 A CN103229674 A CN 103229674A CN 2013101891314 A CN2013101891314 A CN 2013101891314A CN 201310189131 A CN201310189131 A CN 201310189131A CN 103229674 A CN103229674 A CN 103229674A
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Abstract
The invention relates to a method for simply and rapidly identifying the resistance of the pyricularia grisea. The method comprises the steps of: extracting a heading rice plant from the rice field, respectively taking rice internode stems with lengths of not less than 5cm above and below an internode by using the internode of a spike neck for later use; placing degreasing cotton in a moisturizing plastic box, irrigating 8-10ml of spore suspension solution each milliliter of which contains 0.6 millions pyricularia grisea; and placing the rice internode stems to be detected on the degreasing cotton, moisturizing and cultivating for 14 days at a temperature of 25-26 DEG C so that scabs which can be fully identified can be formed, wherein sizes and invasion degrees of the scabs are divided into five levels, the rice internode stems with 0-level or 1-level morphological characteristics are of a disease-resistant type R, the rice internode stems with 4-level morphological characteristics are of a disease-susceptible type S, and the rice internode stems with 2-level or 3-level morphological characteristics are of a middle type M. Identification can be carried out indoors by using a stem node inoculating method, thus errors caused by identification under the natural condition in the field are avoided. The method does not need equipment, and is simple and easy to operate and strong in practicability.
Description
Technical field
The present invention relates to a kind of agriculture production technology, particularly a kind of method of simple and easy Rapid identification rice blast resistance.
Background technology
Rice blast is one of main disease of paddy rice.The whole world is annual because of about 6,000,000,000 dollars of rice blast harm loss paddy.In the popular time of China's rice blast, paddy rice is often wanted the underproduction 10-30%, sometimes even more, seriously influences rice high yield, stable yields.Facts have proved: selecting the disease-resistant paddy rice breeding of high yield for use is the cost-effective measure of control rice blast.
In recent years, the rice breeding plan of many countries is all classified disease-resistant, pest-resistant, breeding for stress tolerance as important goal.Yet want breeding resistant variety, to carry out fast and large-scale the evaluation variety resources of rice at first exactly, screen inhomogeneous anti-source hybrid strain is provided, the material that makes some have specific resistance is in time used, thereby cultivate the new varieties that some sick worm etc. had resistance, apply producing.
For the evaluation of rice blast, mostly adopt seedling stage and artificial infection at heading stage to identify at present.Owing to be the spike of rice seasonal febrile diseases producing what cause the paddy rice heavy losses, the material and the later stage fringe pest resistance degree of correlation that have resistance seedling stage are not high, thereby after identifying seedling stage, the fringe phase still need identify the fringe pest.Adopt in the form ears method of manual injection's inoculation in booting stage of paddy rice the evaluation of fringe pest.Because of rice varieties difference breeding time, weather conditions were also different when inoculated booting stage, and different cultivars fringe type is in different size in addition, caused earing soon, differing slowly, and the inoculation of fringe phase also often causes erroneous judgement.
Summary of the invention
The technical problem to be solved in the present invention is at the deficiencies in the prior art, has proposed a kind of method of simple, practical simple and easy Rapid identification rice blast resistance.
The technical problem to be solved in the present invention is achieved through the following technical solutions, and a kind of method of simple and easy Rapid identification rice blast resistance is characterized in:
Get the rice strain of heading from the field, centered by the internode of panicle neck, on the joint, that joint is down respectively got the paddy rice internode stem that is not less than 5 centimeter length is standby; In the plastic casing of preserving moisture, put absorbent cotton, pour into 8-10 milliliters of magnaporthe grisea spore suspension; Above-mentioned paddy rice internode stem to be measured is placed on the absorbent cotton, under 25-26 ℃ of conditions, preserve moisture and cultivate after 14 days, can form the scab that fully to differentiate;
The preparation of magnaporthe grisea spore suspension: separate the representative pathogenic bacterial strains of cultivating with the locality, after the spore generation is cultivated in expansion, combined inoculation, spore concentration reaches about 600,000 every milliliter at test under microscope;
The judgement of scab degree on the joint: scab size and occurring degree are divided into 5 grades,
0 grade: do not have scab on the internode;
1 grade: produce the brown point of minority on the internode;
2 grades: more brown point is arranged on the internode;
3 grades: more, big foxiness is arranged on the internode;
4 grades: save entirely that blackening, mycelia cover full joint, the stem stalk crumbles,
Paddy rice internode stem with 0 grade or 1 grade morphological feature is disease-resistant type R; Paddy rice internode stem with 4 grades of morphological features is susceptible type S; Paddy rice internode stem with 2 grades or 3 grades morphological features is osculant M.
The present invention compared with prior art utilizes the stipes inoculation method, identifies indoor, has avoided identifying under the natural conditions of field the error of bringing.This method need not equipment, and is simple, quite similar with last scab enlarged degree of time and field kind fringe pest occurring degree because the reaction of internode scab is very consistent with leaf pest reaction in seedling stage, so practicality is very strong.
Embodiment
A kind of method of simple and easy Rapid identification rice blast resistance,
Get the rice strain of heading from the field, centered by the internode of panicle neck, on the joint, that joint is down respectively got the paddy rice internode stem (always being about 10 centimetres) that is not less than 5 centimeter length is standby; In the plastic casing of preserving moisture, put absorbent cotton, pour into 8-10 milliliters of magnaporthe grisea spore suspension; Above-mentioned paddy rice internode stem to be measured is placed on the absorbent cotton, under 25-26 ℃ of conditions, preserve moisture and cultivate after 14 days, can form the scab that fully to differentiate;
The preparation of magnaporthe grisea spore suspension: separate the representative pathogenic bacterial strains of cultivating with the locality, after the spore generation is cultivated in expansion, combined inoculation, spore concentration reaches about 600,000 every milliliter at test under microscope;
The judgement of scab degree on the joint: scab size and occurring degree are divided into 5 grades,
0 grade: do not have scab on the internode;
1 grade: produce the brown point of minority on the internode;
2 grades: more brown point is arranged on the internode;
3 grades: more, big foxiness is arranged on the internode;
4 grades: save entirely that blackening, mycelia cover full joint, the stem stalk crumbles,
Paddy rice internode stem with 0 grade or 1 grade morphological feature is disease-resistant type R; Paddy rice internode stem with 4 grades of morphological features is susceptible type S; Paddy rice internode stem with 2 grades or 3 grades morphological features is osculant M.
Claims (1)
1. the method for a simple and easy Rapid identification rice blast resistance is characterized in that:
Get the rice strain of heading from the field, centered by the internode of panicle neck, on the joint, that joint is down respectively got the paddy rice internode stem that is not less than 5 centimeter length is standby; In the plastic casing of preserving moisture, put absorbent cotton, pour into 8-10 milliliters of magnaporthe grisea spore suspension; Above-mentioned paddy rice internode stem to be measured is placed on the absorbent cotton, under 25-26 ℃ of conditions, preserve moisture and cultivate after 14 days, can form the scab that fully to differentiate;
The preparation of magnaporthe grisea spore suspension: separate the representative pathogenic bacterial strains of cultivating with the locality, after the spore generation is cultivated in expansion, combined inoculation, spore concentration reaches about 600,000 every milliliter at test under microscope;
The judgement of scab degree on the joint: scab size and occurring degree are divided into 5 grades,
0 grade: do not have scab on the internode;
1 grade: produce the brown point of minority on the internode;
2 grades: more brown point is arranged on the internode;
3 grades: more, big foxiness is arranged on the internode;
4 grades: save entirely that blackening, mycelia cover full joint, the stem stalk crumbles,
Paddy rice internode stem with 0 grade or 1 grade morphological feature is disease-resistant type R; Paddy rice internode stem with 4 grades of morphological features is susceptible type S; Paddy rice internode stem with 2 grades or 3 grades morphological features is osculant M.
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| CN2013101891314A CN103229674A (en) | 2013-05-21 | 2013-05-21 | Method for simply and rapidly identifying resistance of pyricularia grisea |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521592A (en) * | 2015-01-04 | 2015-04-22 | 连云港市农业科学院 | Large-scale quantitative and strengthened inoculation authenticating method for paddy black-streaked dwarf disease resistance |
| CN105104147A (en) * | 2015-09-10 | 2015-12-02 | 广西大学 | Method for rapidly inducing rice blast for agar substrate seedling nursery |
| CN105191680A (en) * | 2015-11-02 | 2015-12-30 | 湖北省农业科学院植保土肥研究所 | Indoor rice variety Magnaporthe oryzae resistance identification method |
| CN105284450A (en) * | 2015-09-21 | 2016-02-03 | 广西大学 | Method for quickly inducing rice blast via box type seedling nursing |
| CN105613072A (en) * | 2016-01-20 | 2016-06-01 | 安徽理想种业有限公司 | Identification method of resistance to rice blast |
| CN109988815A (en) * | 2019-04-18 | 2019-07-09 | 扬州大学 | A method of sharp eyespot resistance is identified using the in vitro stalk of rice |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1173273A (en) * | 1996-08-12 | 1998-02-18 | 郑大宽 | Method for integrated control of rice blast |
| CN1326671A (en) * | 2000-06-05 | 2001-12-19 | 华中农业大学 | Breeding method for anti-rice blast rice breed |
| CN101240346A (en) * | 2008-03-13 | 2008-08-13 | 云南农业大学 | Method for analyzing rice blast resisting gene |
| CN102487742A (en) * | 2011-12-05 | 2012-06-13 | 江西省农业科学院植物保护研究所 | Method for identifying rice blast resistance of rice |
-
2013
- 2013-05-21 CN CN2013101891314A patent/CN103229674A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1173273A (en) * | 1996-08-12 | 1998-02-18 | 郑大宽 | Method for integrated control of rice blast |
| CN1326671A (en) * | 2000-06-05 | 2001-12-19 | 华中农业大学 | Breeding method for anti-rice blast rice breed |
| CN101240346A (en) * | 2008-03-13 | 2008-08-13 | 云南农业大学 | Method for analyzing rice blast resisting gene |
| CN102487742A (en) * | 2011-12-05 | 2012-06-13 | 江西省农业科学院植物保护研究所 | Method for identifying rice blast resistance of rice |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521592A (en) * | 2015-01-04 | 2015-04-22 | 连云港市农业科学院 | Large-scale quantitative and strengthened inoculation authenticating method for paddy black-streaked dwarf disease resistance |
| CN104521592B (en) * | 2015-01-04 | 2016-09-07 | 连云港市农业科学院 | Inoculation identification method is quantitatively strengthened in the scale of a kind of black streaked dwarf virus of rice resistance |
| CN105104147A (en) * | 2015-09-10 | 2015-12-02 | 广西大学 | Method for rapidly inducing rice blast for agar substrate seedling nursery |
| CN105284450A (en) * | 2015-09-21 | 2016-02-03 | 广西大学 | Method for quickly inducing rice blast via box type seedling nursing |
| CN105191680A (en) * | 2015-11-02 | 2015-12-30 | 湖北省农业科学院植保土肥研究所 | Indoor rice variety Magnaporthe oryzae resistance identification method |
| CN105191680B (en) * | 2015-11-02 | 2019-04-05 | 湖北省农业科学院植保土肥研究所 | A kind of interior rice varieties blast resistance identification method |
| CN105613072A (en) * | 2016-01-20 | 2016-06-01 | 安徽理想种业有限公司 | Identification method of resistance to rice blast |
| CN109988815A (en) * | 2019-04-18 | 2019-07-09 | 扬州大学 | A method of sharp eyespot resistance is identified using the in vitro stalk of rice |
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Application publication date: 20130807 |