CN105613072A - Identification method of resistance to rice blast - Google Patents

Abstract

The invention discloses an identification method of the resistance to rice blast. The identification method comprises the following steps: (a) after soaking a plurality of rice plants infected with the rice blast in water for 12 hours, putting the rice plants into a constant-temperature box; keeping the temperature at 25 DEG C to 30 DEG C for 24 hours, wherein liquid obtained by washing the rice plants, taken out when pathogenic bacterium conidiums are examined by a microscope, in the water, is rice blast liquid; (b) selecting a white transparent thin film and folding and ironing the thin film into a plurality of plastic bags with the length of 1m and width of 0.5m; (c) injecting the rice blast liquid into spikes of a plurality of healthy rice plants by using a medical injection syringe, and sleeving the rice plants with the plastic bags, wherein each plastic bag sleeves four rice plants and is fixed by using a rope at the lower parts of the sleeved rice plants; (d) after one month, opening the plastic bags, and identifying the morbidity degree of the rice plants. According to the identification method disclosed by the invention, the special transparent plastic bags sleeve the rice plants so that temperature and humidity are increased, a high-temperature and high-humidity environment is created and pathogenic bacteria are effectively induced to breed in a large quantity, and furthermore, the identification effect is realized.

Description

A kind of Rice Resistance To Rice Blast authentication method

Technical field

The present invention relates to field of agricultural production technologies, particularly relate to a kind of Rice Resistance To Rice Blast authentication method.

Background technology

Rice blast is one of Major Diseases of Oryza sativa L.. The whole world is annual because of rice blast damage losses Oryza glutinosa about 6,000,000,000 dollars. In China's rice blast popular time, Oryza sativa L. often wants the underproduction 10 30%, sometimes even more, severely impacts rice high yield, stable yields. Facts have proved that the selection disease-resistant Oryza sativa L. breeding of high yield is the preventing and treating cost-effective measure of rice blast. In recent years, the rice breeding plan of many countries is all classified as disease-resistant, pest-resistant, breeding for stress tolerance as important goal. But want breeding resistant variety, first seek to carry out quickly and on a large scale identifying to variety resources of rice, screen inhomogeneous anti-source and hybrid strain is provided, some materials with specific resistance are made to be applied in time, thus cultivating the new varieties that some disease pest etc. is had resistance, popularization and application on producing.

At present, rice blast authentication method generally has artificial infection idenfication and spontaneous induction to identify. Artificial infection idenfication is to spray rice blast pathogen at rice ear sprouting period, allows its infection morbidity, carries out classification identification according to spike of rice cervical region occurring degree. But this method is affected greatly by humiture, if spraying rice blast pathogen after humiture low, pathogenic bacteria cannot survival and reproduction, affect identification result.

Summary of the invention

Invention problem to solve is that: providing a kind of Rice Resistance To Rice Blast authentication method, it has qualification result is not affected by humiture, and qualification result is feature more accurately.

This invention address that technical problem adopts the following technical scheme that

A kind of Rice Resistance To Rice Blast authentication method, comprises the steps:

A, configuration rice blast bacterium solution: some strain sense rice blast rice strains are placed in water and soak after 12 hours put into calorstat, keep 25-30 DEG C, time is 24 hours, after seeing pathogenic bacteria conidium with sediments microscope inspection, take out some strain sense rice blast rice strains be placed on water rinses after the liquid that obtains be rice blast bacterium solution;

B, make specialty transparent plastic bag: select white clear thin film being folded press into 1 meter long 0.5 meter of wide plastic bag several;

C, use injector for medical purpose inject some strain health rice ear parts rice blast bacterium solution, plastic bag cover in described rice strain, and the strain of each plastic bag cover 4, at described rice plant bottom rope, plastic bag is fixed after entangling, it is prevented that plastic bag comes off;

D, after one month, open plastic bag, identify described rice strain occurring degree.

The invention has the beneficial effects as follows: the present invention is directed to the deficiencies in the prior art, adopt dedicated transparent plastic bag, entangle rice plant, increase humiture, creating hot and humid environment, effectively bringing out pathogenic bacteria amount reproduction, thus reaching identification result.

Detailed description of the invention

Below in conjunction with specific embodiment, the present invention is described in further details:

Embodiment 1

A, configuration rice blast bacterium solution: some strain sense rice blast rice strains are placed in water and soak after 12 hours put into calorstat, keep 25-30 DEG C, time is 24 hours, after seeing pathogenic bacteria conidium with sediments microscope inspection, take out some strain sense rice blast rice strains be placed on water rinses after the liquid that obtains be rice blast bacterium solution;

B, make specialty transparent plastic bag: select white clear thin film being folded press into 1 meter long 0.5 meter of wide plastic bag several;

C, use injector for medical purpose inject some strain health rice ear parts rice blast bacterium solution, plastic bag cover in described rice strain, and the strain of each plastic bag cover 4, at described rice plant bottom rope, plastic bag is fixed after entangling, it is prevented that plastic bag comes off;

D, after one month, open plastic bag, identify described rice strain occurring degree.

The present invention proposes a kind of Rice Resistance To Rice Blast authentication method, and it adopts dedicated transparent plastic bag, entangles rice plant, increases humiture, creating hot and humid environment, effectively bringing out pathogenic bacteria amount reproduction, thus reaching identification result. The present invention has simple to operate, and cost of manufacture is low, brings out rice blast effective, is a simple efficient blast resistance identification method.

The above; it is only the present invention preferably detailed description of the invention; but protection scope of the present invention is not limited thereto; any those familiar with the art is in the technical scope that the invention discloses; it is equal to replacement according to technical scheme and inventive concept thereof or is changed, all should be encompassed within protection scope of the present invention.

Claims (1)

1. a Rice Resistance To Rice Blast authentication method, it is characterised in that comprise the steps:

A, configuration rice blast bacterium solution: some strain sense rice blast rice strains are placed in water and soak after 12 hours put into calorstat, keep 25-30 DEG C, time is 24 hours, after seeing pathogenic bacteria conidium with sediments microscope inspection, take out some strain sense rice blast rice strains be placed on water rinses after the liquid that obtains be rice blast bacterium solution;

B, make specialty transparent plastic bag: select white clear thin film being folded press into 1 meter long 0.5 meter of wide plastic bag several;

C, use injector for medical purpose inject some strain health rice ear parts rice blast bacterium solution, plastic bag cover in described rice strain, and the strain of each plastic bag cover 4, at described rice plant bottom rope, plastic bag is fixed after entangling, it is prevented that plastic bag comes off;

D, after one month, open plastic bag, identify described rice strain occurring degree.