CN1173273A - Method for integrated control of rice blast - Google Patents
Method for integrated control of rice blast Download PDFInfo
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- CN1173273A CN1173273A CN96106468A CN96106468A CN1173273A CN 1173273 A CN1173273 A CN 1173273A CN 96106468 A CN96106468 A CN 96106468A CN 96106468 A CN96106468 A CN 96106468A CN 1173273 A CN1173273 A CN 1173273A
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Abstract
A comprehensive method for preventing and eliminating rice blast features use of a set of distinguishing varieties for rice blast bacteria and a set of analysis, calculation, preventing and eliminating means, and the combination of bacteria monitor, resistance discrimination and forecast, resulting in high level to prevent and eliminate rice blast.
Description
The present invention relates to a kind of method for integrated control of rice disease.
At present, the sick garden that prevents and treats rice blast both at home and abroad is Dan Pudan usefulness, and the method for employing is simpler and cruder, is mainly used in prediction rice blast then, function singleness.The kind that adopt in sick garden is few, and plantation distributes also corresponding simpler, generally only adopt local susceptible variety and mainly plant one, so the accuracy of the scope of prediction rice blast, degree, the population of causing a disease is poor slightly.The employing kind is few, can't carry out varietal resistance and identify that investigation method is loaded down with trivial details, and systematicness is relatively poor, can not well provide reliable basis for integrated control.
The objective of the invention is provides multiduty rice blast garden in order to overcome above-mentioned defective, is used to monitor the Pyricularia oryzae population, the method for the integrated control rice blast of prediction rice blast and identification of species resistance.
The object of the present invention is achieved like this:
At local rice blast Chang Faqu, select a suitable field piece as sick garden, adopt conventional measure to manage the disease garden, bring out each the kind morbidity of disease garden.Kind that adopts and cultivation distribute as follows:
(1) adopts a cover Chinese rice blast fungus biological strain differential variety, be that Te Tebo, precious dragon 13, four are rich 43, east farming 363, No. 51, the Northeast, No. 18, Hejiang, Lijiang xintuanheigu, with local main breed, the kind of succeeding, introduced variety several, but be not less than 6,1 of disease indicator variety, 1 of susceptible variety.
(2) in the rice seedling bed around the sick garden, each kind is broadcast a district, and systematic arrangement moves into the disease garden in good time.
(3) sub-district, sick garden is arranged as: gross area 530-667 square metre, tested variety is respectively established a sub-district, every sub-district plants 4 row, every row is more than 10 clumps, wherein main breed, the kind of succeeding, introduced variety, the every kind of differential variety are only planted 2 row, plant susceptible variety again on one side, another side is planted 1 row indicator variety.Plant 13 * 20 centimetres of specifications, every minizone is every 0.3～0.5 meter, repeats twice, systematic arrangement for the first time, and inverted order is arranged for the second time, and two stay 0.5～0.6 meter work row between repeating, and plant the above susceptible variety of 6 row around the field and make guard row.
Sick garden of the present invention except than the stronger function of conventional sick prediction of assisting, also has the effect that disease garden flora monitoring and varietal resistance are identified concurrently when using.The three is tight inseparable organic whole.
Sick garden flora monitoring and varietal resistance authentication method and prediction methods are as follows:
(1) resistance standard of perfection: the anti-perception of progression code name (A) seedling, leaf pest reaction (B) fringe pest response type
High anti-brown some fringe pest of the anosis secondary R disease-resistant spot syringe needle shape rate of type grade scale grade scale one-level HR is lower than anti-little and oval downright bad fringe pest rate 1.0%～5.0% among 1.0% 3 grade of MR
Gray corrosion, wide pact
1～2 millimeter, the edge
The typical scab spindle-type of sense among the brown level Four MS, fringe pest rate 5.0%～10.0%
Long 1～2 centimetre, office
Be limited to that Pyatyi S feels typical scab between two veins, sick class fringe pest rate 10.0%～25.0%
Size surpasses two veins
Between, sick class causes harm
Leaf area is no more than the typical scab of the high sense of 50% 6 grade of IIS, and scab fringe pest rate is more than 25.0%
The leaf area that causes harm 50% with
On, most blades are withered
(2) control time and method:
The leaf pest are in morbidity phase beginning, and peak period and basicly stable phase respectively look into once, and the fringe pest reach in the watery stage respectively to be looked into once stage of yellow ripeness.Each 10 clumps of top, sub-district threeleaf loosestrife herb sheet or whole spike of rice are investigated in the sampling of " it " font at every turn, and record data;
(3) germ grouping method:
Carrying out the germ microspecies according to differential variety resistance response type hives off, rich 43, the east farming 363 of corresponding Te Tebo, precious dragon 13, four respectively of A, B, C, D, E, F, G kind, the Northeast 51, Hejiang 18, Lijiang xintuanheigu, the differential variety that the A kind is planted for a ZA group of mean people, the genus ZA group of all susceptible reactions of performance on the A kind; The B kind is the differential variety that a ZB group of mean people plants, allly show disease resistance response on the A kind, and the susceptible reaction of performance on the B kind belongs to ZB group; The C kind is the differential variety that a ZC group of mean people plants, all genus ZC groups who A, B kind performance disease resistance response is showed susceptible reaction to the C kind; By that analogy, respectively adhere to ZD, ZE, ZF, ZG group separately, when being disease resistance response, belong to ZH group as if 7 differential varieties.
Table 1 Chinese rice blast fungus biological strain flora ideograph
| Differential variety kind code name response type flora code name | Te Tebo | Precious dragon 13 | Four rich 43 | East farming 363 | The Northeast 51 | Hejiang 18 | Lijiang xintuanheigu |
| ????A | ????B | ????C | ????D | ????E | ????F | ????G | |
| ????ZA ????ZB ????ZC ????ZD ????ZE ????ZF ????ZG ????ZH | ????S ????R ????R ????R ????R ????R ????R ????R | ????S ????R ????R ????R ????R ????R ????R | ????S ????R ????R ????R ????R ????R | ????S ????R ????R ????R ????R | ????S ????R ????R ????R | ????S ????R ????R | ????S ????R |
(4) computational methods:
The local morbidity gross area=∑ (all types of fields onset area percentage * all types of fields area)
(5) popular degree criteria for classifying of prediction disease:
The light generation: should prevent that area accounts for the gross area below 5%
In send out partially gently: should prevent that area accounts for the gross area 5%～10%
The middle generation: should prevent that area accounts for the gross area 10%～20%
In send out and to lay particular stress on: should prevent that area accounts for the gross area 20%～30%
Retransmit and give birth to: should prevent that area accounts for the gross area more than 30%
(6) the land for growing field crops range estimation generaI investigation occurring degree criteria for classifying:
0 is anosis: range estimation field piece is not seen morbidity
+ light generation the: the visible fragmentary morbidity of range estimation field piece, the morbidity field rarely seen fragmentary sick leaf of piece (fringe)
++ middle generation: range estimation field piece has the field piece morbidity about 10%, the morbidity field sick leaf of piece (fringe) show and
Easily see
+++retransmit to give birth to: range estimation field piece has the visible tangible infection center of the field piece morbidity 20% or more or
Sick class of many acutes or more dead ears.
The present invention has adopted a cover Chinese rice blast fungus differential variety, the kind that is aided with other multiple classes again, and generally investigate in conjunction with the land for growing field crops, with prediction and sick garden flora monitoring, varietal resistance is identified and is organically combined, change the method for the single usefulness in the past single garden, saved a large amount of human and material resources, improved the technical merit of integrated control rice blast.
Describe the present invention in detail below in conjunction with embodiment:
Embodiment: 1, at local rice blast Chang Faqu, select to go up in the fertility, irrigation and drainage 667 square metres of sick gardens of conduct of field piece easily.Tested variety is planted into the rice seedling bed, and each kind is broadcast a district, and systematic arrangement moves in the sick garden more in good time.Whole process is taked in good time early sowing, and seed is not sterilized, and suitably enriches nitrogenous fertilizer, does not execute the agricultural chemicals that rice blast is had preventive effect, and other are by local Routine Management.Tested variety is local main breed, each 2 of the kinds of succeeding, introduced variety, 1 of disease indicator variety, height 1 of susceptible variety, differential variety one cover, wherein differential variety one cover, east farming 363 rich 43, the Northeast 51, No. 18, Hejiang, Lijiang xintuanheigu for Te Tebo, precious dragon 13, four.Tested variety is respectively established 1 sub-district, and 4 row, 10 clumps of every row are planted in every sub-district; wherein main breed, the kind of succeeding, introduced variety and the every kind of differential variety are only planted 2 row; plant 1 row susceptible variety again on one side, another side is planted 1 row indicator variety, plants 13 * 20 centimetres of specifications; every minizone is every 0.4 meter; repeat twice, systematic arrangement for the first time, inverted order is arranged for the second time; stay 0.6 meter work row between two repetitions, the susceptible variety of planting around the field more than 6 row is made guard row.
Adopt relevant sick garden flora monitoring of the present invention and varietal resistance authentication method and prediction methods continuous monitoring 5 years, monitoring result and application result are as follows:
Monoid that biological strain occurs and frequency be early rice except that ZA group does not occur, other 6 floras all occur, and ZG group, frequency reaches 100%; Next ZC group, ZF group, the frequency of appearance is respectively 90% and 80%; ZD group 50%; ZE group and ZB group respectively are 30% and 20%.The frequency sequence of its flora can be arranged as: ZG＞ZC＞ZF＞ZD＞ZE＞ZB.6 outer floras of ZA group have also appearred in late rice, and ZD group and ZG group's frequency of occurrences all are 100%, and ZF group 75%, and ZB, ZC are 50%.See table 1 for details
Dominant microflora and the dominant population that is changed to early rice leaf pest thereof are ZC, ZG group and have relative stability over 5 years.And early the dominant population of rice blast fringe changes greatly, and except that ZG group, 1 year and 1 year is ZB group, and the 3rd year and the 4th year is ZC group, and the 5th year is ZF group.
The dominant population of late rice leaf pest is except that ZG group, and the 4th year is ZD, and the 5th year is ZB; The dominant population of fringe pest is except that ZG group, and the 4th year is ZD, and the 5th year is ZB.Illustrate that ZB group is rising.
The result that varietal resistance is identified show the early rice resistance have preferably Hunan early 1 day red morning of No. 1, Xian, Lu, 78-130, M1460, prestige kind such as excellent 64, association excellent 64.All performance is more anti-at second and third, in four the sick garden in 3 years for prestige excellent 64, sick leaf rate 16～19%, and disease index 3.2-4, three the fringe pest do not take place every year.Hunan is sick leaf rate 27% of Xian early, and the fringe pest did not take place disease index in 5.4, three years yet.Resistance descends the capital 63-1 of section, and the 3rd year is IIR, and the 4th year is R, the 5th year MS; Shan was IIR in excellent 64, the four years, and the 5th year is MS.The early rice susceptible variety has Er Jiufeng, red prominent 31, and the capital is wide by 75, and 73-07, Zhejiang spoke 802, M79007, M831113, prestige are excellent 17, prestige is excellent 48, prestige excellent 49.
The late rice resistance has preferably that Shanyou 63, association are excellent 63, prestige is excellent 64, corn rice, and susceptible variety has that prestige is excellent 49, prestige is excellent 48, prestige is excellent 17, Shan excellent 64.(seeing table 3 for details)
Table 1 early, situation appears in late rice Pyricularia oryzae population
| Rice season is other | Time | The morbidity stage | Microspecies flora code name | ||||||
| ZA | ZB | ZC | ZD | ZE | ZF | ZG | |||
| Early rice | 1 year | Leaf pest fringe pest | △ | △ △ | △ | △ △ | △ △ | ||
| 1 year | Leaf pest fringe pest | △ | △ △ | △ | △ | △ | △ △ | ||
| The 3rd year | Leaf pest fringe pest | △ △ | △ | △ | △ △ | ||||
| The 4th year | Leaf pest fringe pest | △ △ | △ | △ | △ △ | △ △ | |||
| The 5th year | Leaf pest fringe pest | △ | △ | △ | △ △ | △ △ | |||
| Microspecies class group frequency microspecies class group frequency | 0 0 | 2 20 | 9 90 | 5 50 | 3 30 | 8 80 | 10 100 | ||
| Late rice | The 4th year | Leaf pest fringe pest | △ △ | △ | △ | △ △ | |||
| The 5th year | Leaf pest fringe pest | △ △ | △ △ | △ △ | △ | △ △ | △ | ||
| Microspecies monoid frequency microspecies class group frequency | 0 0 | 2 20 | 2 50 | 4 100 | 2 50 | 3 75 | 4 100 | ||
| Two seasons added up to | Microspecies monoid frequency microspecies class group frequency | 0 0 | 4 28.57 | 11 78,57 | 9 64.29 | 5 35.71 | 10 71.42 | 14 100 | |
This group of " △ " symbology differential variety response type is more than " MS " level in the table.
Table 2 Pyricularia oryzae dominant microflora situation of change
| Rice season is other | The morbidity stage | Each year, situation appearred in dominant microflora | ||||
| 1 year | 1 year | The 3rd year | The 4th year | The 5th year | ||
| Early rice | Leaf pest fringe pest | ??ZG????ZC ??ZG????ZB | ??ZG????ZC ??ZG????ZB | ??ZG????ZC ??ZG????ZC | ??ZG????ZC ??ZG????ZC | ??ZG????ZC ??ZG????ZF |
| Late rice | Leaf pest fringe pest | ??ZG????ZD ??ZG????ZB | ??ZG????ZD ??ZG????ZB | |||
Table 3 rice varieties field resists the classification of (sense) pest
| Time | ????HR | ????R | ??MR | ????MS | ??S | ?HS |
| 1 year | ??M831459 ??M831456 | ????M79007 ????M831113 | The excellent wheat of F883008 early 61266113 | 74-1068 7004,6,001 1511, No. 14 Shans of Xian morning are spent 369 pioneers No. one | ||
| 1 year | 78-130 prestige excellent 64 | The capital 63-1 of section | Red prominent 31 | Two or nine is rich | Gradually spoke 802 78-1000 pioneers No. one | |
| The 3rd year | No. one, red prominent 31 Hunan Xian morning of the excellent 64 capital 63-1 of section of prestige | Two or nine is rich | Pioneer No. one | |||
| The 4th year | Excellent 64 associations excellent 49 of excellent 64 associations of the excellent 64 Shanyou 63 Shans of prestige | The excellent excellent 63 capital 63-1 of section of 49 associations of excellent 48 prestige of prestige | The excellent 17 Zhejiang spokes of prestige 802 Lus fourth four one day red morning, 73-07 | Capital wide 75 29 is rich | Pioneer No. one | |
| The 4th year (late rice) | Corn rice | Excellent 64 Shanyou 63s of prestige | Excellent 49 associations excellent 64 of excellent 63 associations of excellent 49 associations of prestige | Excellent 17 prestige excellent 48 of prestige | ||
| The 5th year | Excellent 64 prestige excellent 19 of excellent 64 Shans of prestige are gargled Hunan 142 78-130 | Assist excellent 49 Shanyou 63s to assist excellent 63 1 day red morning of M1450 MY82166 Shanghai | The capital 63-1 73-07 of section | Pioneer No. one | ||
| The 5th year (late rice) | Prestige excellent 49 | Excellent 49 associations excellent 63 of excellent 64 associations of M1460 MY82166 Shanyou 63 prestige | Shan excellent 64 | Gargle Hunan 142 |
Claims (1)
1, a kind of method for integrated control of rice blast is characterized in that:
(1) adopts a cover Chinese rice blast fungus biological strain differential variety, be that Te Tebo, precious dragon 13, four are rich 43, east farming 363, No. 51, the Northeast, No. 18, Hejiang, Lijiang xintuanheigu, with local main breed, the kind of succeeding, introduced variety several, but be not less than 1,1 of disease indicator variety, 1 of susceptible variety.
(2) in the rice seedling bed around the sick garden, each kind is broadcast a district, and systematic arrangement moves into the disease garden in good time.
(3) sub-district, sick garden is arranged as: gross area 530-667 square metre, tested variety is respectively established a sub-district, every sub-district plants 4 row, every row is more than 10 clumps, wherein main breed, the kind of succeeding, introduced variety, the every kind of differential variety are only planted 2 row, plant susceptible variety again on one side, another side is planted 1 row indicator variety.Plant 13 * 20 centimetres of specifications, every minizone is every 0.3～0.5 meter, repeats twice, systematic arrangement for the first time, and inverted order is arranged for the second time, and two stay 0.5～0.6 meter work row between repeating, and plant the above susceptible variety of 6 row around the field and make guard row.
(4) sick garden flora monitoring and varietal resistance authentication method and prediction methods are as follows:
Resistance standard of perfection: the anti-perception of progression code name (A) seedling, leaf pest reaction (B) fringe pest response type
High anti-brown some fringe pest of the anosis secondary R disease-resistant spot syringe needle shape rate of type grade scale grade scale one-level IIR is lower than anti-little and oval downright bad fringe pest rate 1.0%～5.0% among 1.0% 3 grade of MR
Gray corrosion, wide pact
1～2 millimeter, the edge
The typical scab spindle-type of sense among the brown level Four MS, fringe pest rate 5.0%～10.0%
Long 1～2 centimetre, office
Be limited to that Pyatyi S feels typical scab between two veins, sick class fringe pest rate 10.0%～25.0%
Size surpasses two veins
Between, sick class causes harm
Leaf area is no more than the typical scab of the high sense of 50% 6 grade of HS, and scab fringe pest rate is more than 25.0%
The leaf area that causes harm 50% with
On, most blades are withered
Control time and method:
The leaf pest are in morbidity phase beginning, and peak period and basicly stable phase respectively look into once, and the fringe pest reach in the watery stage respectively to be looked into once stage of yellow ripeness.Each 10 clumps of top, sub-district threeleaf loosestrife herb sheet or whole spike of rice are investigated in the sampling of " it " font at every turn, and record data;
The germ grouping method:
Carrying out the germ microspecies according to differential variety resistance response type hives off, rich 43, the east farming 363 of corresponding Te Tebo, precious dragon 13, four respectively of A, B, C, D, E, F, G kind, the Northeast 51, Hejiang 18, Lijiang xintuanheigu, the differential variety that the A kind is planted for a ZA group of mean people, the genus ZA group of all susceptible reactions of performance on the A kind; The B kind is the differential variety that a ZB group of mean people plants, allly show disease resistance response on the A kind, and the susceptible reaction of performance on the B kind belongs to ZB group; The C kind is the differential variety that a ZC group of mean people plants, all genus ZC groups who A, B kind performance disease resistance response is showed susceptible reaction to the C kind; By that analogy, respectively adhere to ZD, ZE, ZF, ZG group separately, when being disease resistance response, belong to ZH group as if 7 differential varieties.
Computational methods:
The local morbidity gross area=∑ (all types of fields onset area percentage * all types of four sides are long-pending)
Prediction disease popular degree criteria for classifying: the light generation: it is partially light with lower to prevent that area accounts for the gross area 5%: should prevent that area accounts in the gross area 5%～10% occurs: should prevent that area accounts for to send out in the gross area 10%～20% and lay particular stress on: should prevent that area accounts for the gross area 20%～30% and retransmits and give birth to: should prevent that area accounts for the gross area 30% above land for growing field crops range estimation generaI investigation occurring degree criteria for classifying: 0 is anosis: the range estimation field has no morbidity+light generation: as seen the range estimation field sporadicly falls ill; The morbidity rarely seen fragmentary sick leaf of field (fringe) ++ middle generation: the range estimation field has the field morbidity about 10%, the morbidity sick leaf of field (fringe) aobvious and
Easily see +++retransmit to give birth to: range estimation field piece has the visible tangible infection center of the field piece morbidity 20% or more or
Sick class of many acutes or more dead ears.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96106468A CN1173273A (en) | 1996-08-12 | 1996-08-12 | Method for integrated control of rice blast |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96106468A CN1173273A (en) | 1996-08-12 | 1996-08-12 | Method for integrated control of rice blast |
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|---|---|
| CN1173273A true CN1173273A (en) | 1998-02-18 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103229674A (en) * | 2013-05-21 | 2013-08-07 | 江苏胜田农业科技发展有限公司 | Method for simply and rapidly identifying resistance of pyricularia grisea |
| CN104381125A (en) * | 2014-11-19 | 2015-03-04 | 长江大学 | Authentication method of rice blast disease resistant breeding material |
| CN106508336A (en) * | 2016-09-23 | 2017-03-22 | 湖北省农业科学院经济作物研究所 | Identification method for clubroot disease resistance of high mountain radishes in field |
| CN111727784A (en) * | 2020-07-27 | 2020-10-02 | 贵州省烟草科学研究院 | Method for forecasting tobacco black shank initial period |
-
1996
- 1996-08-12 CN CN96106468A patent/CN1173273A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103229674A (en) * | 2013-05-21 | 2013-08-07 | 江苏胜田农业科技发展有限公司 | Method for simply and rapidly identifying resistance of pyricularia grisea |
| CN104381125A (en) * | 2014-11-19 | 2015-03-04 | 长江大学 | Authentication method of rice blast disease resistant breeding material |
| CN106508336A (en) * | 2016-09-23 | 2017-03-22 | 湖北省农业科学院经济作物研究所 | Identification method for clubroot disease resistance of high mountain radishes in field |
| CN111727784A (en) * | 2020-07-27 | 2020-10-02 | 贵州省烟草科学研究院 | Method for forecasting tobacco black shank initial period |
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