CN105273992B - A kind of erecting device of DNA sequencer reaction chip - Google Patents
A kind of erecting device of DNA sequencer reaction chip Download PDFInfo
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- CN105273992B CN105273992B CN201510716204.XA CN201510716204A CN105273992B CN 105273992 B CN105273992 B CN 105273992B CN 201510716204 A CN201510716204 A CN 201510716204A CN 105273992 B CN105273992 B CN 105273992B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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Abstract
The present invention relates to the erecting device of a kind of DNA sequencer reaction chip, reaction chip is arranged in reaction warehouse assembly, described reaction warehouse assembly includes turning warehouse, in the inner side of the centre of described turning warehouse, reaction chip assembly is installed, accordingly, the centre at turning warehouse is provided with the first cavity accommodating reaction chip assembly；Be provided with the first boss in the bottom of the first cavity, the first boss is in order to carry described reaction chip assembly；Be provided with the second boss in the upper end of the first described boss, the second boss is in order to position described support member；The second described boss upper end is provided with locating surface, locating surface is provided with locking screw nail.The erecting device of the DNA sequencing reaction chip of the present invention has the robust structure of many recanalization, is provided with the mounting structure of reaction chip assembly, is equiped with reaction chip in reaction chip assembly in turning warehouse；Between each structure easy for installation, compact conformation.
Description
Technical field
Invention relates to DNA sequencing field, particularly relates to the erecting device of a kind of DNA sequencer reaction chip.
Background technology
DNA sequencing operating process of the prior art is described as follows: after DNA sample is by crushing, application is built storehouse reagent and is carried out adding joint, strand captures, it is bound to microsphere, microemulsion PCR expands, breakdown of emulsion liquid, obtain the DNA library set up on microsphere, application adds model by laies such as the enzymes of library and sequencing reaction needs to the sequence testing chip with micro reaction pool, sequence testing chip and sequencing reagent are installed to main frame, order-checking program is started according to module number and position by controlling computer, automatization carries out sequencing reaction, the data produced are transmitted to Data Analysis Computer, after completing order-checking, computation analysis software carries out image procossing, sequence reads, quality analysis, the work such as sequence assembly, finally give the sequence information of DNA sample.It is carved with the sequence testing chip of micro reaction pool to be carried out machine glazing core layer etching by the fibre faceplate of core diameter 25 μ m thick 2mm and obtain, etching depth 40 μm, chip amounts to about 3,000,000 micro reaction pools, wherein about 1,200,000 micro reaction pools of imaging moiety.Micro reaction pool sequence testing chip is the carrier of sequencing reaction, and the DNABeads and the various sequencing reaction enzyme that are loaded with sequencing template are respectively positioned in the sequence testing chip being carved with micro reaction pool.
In sequencing procedure, sequence testing chip occurs chemical reaction, produces visible ray, by optical signal produced by CCD (ChargeCoupleDevice) cameras capture sequencing reaction, i.e. available required order-checking information.In measuring technology, reaction warehouse, as the main carriers of test reaction, should have enough stability and operability, it is possible to according to actual reaction condition, convenient adjustment.
In view of drawbacks described above, creator of the present invention obtains this creation finally through research for a long time and practice.
Summary of the invention
It is an object of the invention to provide the erecting device of a kind of DNA sequencer reaction chip, in order to overcome above-mentioned technological deficiency.
For achieving the above object, the present invention provides the erecting device of a kind of DNA sequencer reaction chip, reaction chip is arranged in reaction warehouse assembly, described reaction warehouse assembly includes turning warehouse, in the inner side of the centre of described turning warehouse, reaction chip assembly is installed, accordingly, the centre at turning warehouse is provided with the first cavity accommodating reaction chip assembly；
Be provided with the first boss in the bottom of the first cavity, the first boss is in order to carry described reaction chip assembly；Be provided with the second boss in the upper end of the first described boss, the second boss is in order to positioning supporting part；The second described boss upper end is provided with locating surface, locating surface is provided with locking screw nail；
Described reaction chip assembly includes reaction chip mounting seat, reaction warehouse base assembly and the shading positioning component that reaction warehouse base assembly and reaction chip mounting seat are locked, opened；Mid portion in reaction chip mounting seat is provided with reaction chip, is provided with cover plate on reaction chip；In the both sides of reaction chip, chute pressing plate is installed；
In the medial surface of reaction warehouse, it is provided with reaction cell body, reaction tank is set at reaction cell body mid portion；Mid portion at the lateral surface of reaction warehouse base is provided with temperature control assembly, and the mid portion at reaction warehouse base is provided with temperature sensor mounting groove；
It is respectively arranged with Reagent Tube at the two ends of reaction tank and screwed hole is installed, in order to be connected with Reagent Tube；The end installing screwed hole at Reagent Tube is provided with inlet and liquid outlet, and liquid inlet and outlet communicates with the two ends of reaction tank；
Avris in the interior grooves of reaction tank is provided with sealing ring, is provided with reaction chip in the centre of reaction chip mounting seat, and reagent flows through reaction chip and reacts.
Further, the side portion in described reaction chip mounting seat is provided with mounting seat and props up support component, and it is in order to taking out or during chip placement, the reaction chip mounting seat described in support；
Described mounting seat is propped up support component and is included torsional-spring supported portion, is arranged in mounting seat, and described torsional-spring supported portion is one with the recess of protruding end, and when the reaction chip mounting seat described in rotating, protruding end contacts with reaction warehouse base assembly at first.
Further, described mounting seat is propped up support component and is also included that torsion spring is connected and is arranged on the tension axle at torsion spring two ends with torsion spring, and described tension axle is arranged in lobe, when starting reaction chip mounting seat, described torsional-spring supported mounting seat, and finally rely on the location of protruding end；
It is additionally provided with the first rotary shaft hole, in order to be connected with reaction warehouse base in the end of described lobe；Sidepiece in reaction chip mounting seat is additionally provided with locating slot.
Further, one end of described reaction warehouse base is provided with raised line, in order to install after cover plate location；
The other end is provided with the torsional-spring supported structure corresponding with reaction chip mounting seat, and it includes symmetrically arranged two protruding ends, is provided with the second rotary shaft hole thereon, and it is coaxial with the first rotary shaft hole, and rotary shaft sequentially passes through holes；It is additionally provided with the region movable for torsion spring and region, location in the end of the reaction warehouse base other end, is provided with in this region in order to the screwed hole by installing reaction warehouse cover plate.
Further, the upper surface in the first receiving chamber of described turning warehouse is provided with Reagent Tube installing hole and electric wire installing hole, in Reagent Tube installing hole, pipe clamp is installed.
Compared with prior art the invention have the benefit that the erecting device of the DNA sequencing reaction chip of the present invention has the robust structure of many recanalization, turning warehouse is provided with the mounting structure of reaction chip assembly, in reaction chip assembly, is equiped with reaction chip；
Between each structure easy for installation, compact conformation, stability is high.Reaction warehouse assembly can install, fixing and reaction chip described in adjusting, it is ensured that reaction chip reacts in stable environment, and, reaction chip can conveniently take out and lock.
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of the DNA sequencer reacting record subsystem of the present invention；
Fig. 2 is the first perspective view of the reaction warehouse assembly of the present invention；
Fig. 3 is the second perspective view of the reaction warehouse assembly of the present invention；
Fig. 4 be the reaction warehouse assembly of the present invention face structural representation；
Fig. 5 a is the perspective view of the turning reaction warehouse assembly of the present invention；
Fig. 5 b is the first perspective view of the turning warehouse of the present invention；
Fig. 5 c is the second perspective view of the turning warehouse of the present invention；
Fig. 5 d is the first perspective view of the locating piece of the present invention；
Fig. 5 e is the second perspective view of the locating piece of the present invention；
Fig. 6 a is the structural representation of the electronic lock of the present invention；
Fig. 6 b is the structural representation of the electronics lock of the present invention；
Fig. 6 c is the sectional structure schematic diagram of the electronic lock of the present invention；
Fig. 7 is the turning reaction warehouse assembly sectional structure schematic diagram along the first lug of the present invention；
Fig. 8 is the 3rd perspective view of the turning warehouse of the present invention；
Fig. 9 a is the structural representation of the reaction chip assembly of the present invention；
Fig. 9 b is the structural representation of the reaction chip mounting seat of the present invention；
Fig. 9 c is the structural representation of the medial surface of the reaction warehouse base of the present invention；
Fig. 9 d is the structural representation of the lateral surface of the reaction warehouse base of the present invention；
Fig. 9 e is the structural representation of the temperature control assembly cover plate of the present invention；
Figure 10 a is the structural representation of the shading positioning component of the present invention；
Figure 10 b is the first structural representation of the slidably positioning and locking block of the present invention；
Figure 10 c is the second structural representation of the slidably positioning and locking block of the present invention；
Figure 10 d is the structural representation of the turnable basement assembly of the present invention；
Figure 10 e is the structural representation of the spring assembly of the present invention；
Figure 11 a is the sectional structure schematic diagram along liquid inlet and outlet of the reaction chip assembly of the present invention；
Figure 11 b is the turning reaction warehouse assembly sectional structure schematic diagram along self-adaptative adjustment part of the present invention；
Figure 11 c is the turning reaction warehouse assembly sectional structure schematic diagram along guide post of the present invention；
Figure 12 is that the turning reaction warehouse assembly of present invention A-A along Fig. 4 is to sectional structure schematic diagram；
Figure 13 a is the sectional structure schematic diagram of the back shroud of the present invention；
Figure 13 b is the perspective view of the back shroud assembly of the present invention；
Figure 13 c is the perspective view of the back shroud of the present invention.
Detailed description of the invention
Below in conjunction with accompanying drawing, to the present invention, above-mentioned and other technical characteristic and advantage are described in more detail.
Refer to shown in Fig. 1, it is the overall structure schematic diagram of DNA sequencer reacting record subsystem of the present invention, reacting record subsystem in the embodiment of the present invention includes reaction warehouse assembly 12, motion platform assembly 14, motion control unit 15, CCD camera 101 and platform assembly 13, wherein
Described reaction warehouse assembly 12, arranges reaction chip assembly in it, under the effect of control system, reactant liquor enters and carries out chemical reaction in reaction chip, produces visible ray；Described motion platform assembly 14, CCD camera is fed into suitable picture-taking position by it, collection of taking pictures the visible ray information produced in reaction chip；Described platform assembly 13, its support described in reaction warehouse assembly 12, motion platform assembly 14；Described motion control unit, the operation of CCD camera and reaction chip is controlled by real time.
In embodiments of the present invention, described reaction warehouse assembly 12 can install, fixing and reaction chip described in adjusting, it is ensured that reaction chip reacts in stable environment, and, reaction chip can conveniently take out and lock.
Refer to shown in Fig. 2-4, described reaction warehouse assembly 12 includes the door frame 121 being vertically arranged being arranged on described platform assembly 13, turning reaction warehouse assembly 124 it is provided with on the top in the outside of door frame 121, connect in the lower end of turning reaction warehouse assembly 124 and have turnable basement 122, turnable basement 122 is fixed on the middle part of door frame 121, turning reaction warehouse assembly 124 can rotate around described turnable basement 122, in order to take out or to put into reaction chip.
It is additionally provided with electronic lock assembly 125 in the left side of the upper end of described door frame 121, only when electronic lock assembly 125 is opened, turning reaction warehouse assembly 124 can be rotated, reaction chip is operated.In embodiments of the present invention, it is provided with door frame 121, supports reaction chip and dependency structure, substantially increase the stability of system.
Turning reaction warehouse assembly 124 includes turning warehouse, and the mid portion at described turning warehouse is provided with back shroud 1242, in order to encapsulate Reagent Tube, and adjusts reaction warehouse assembly and the position of turning warehouse by self-adaptative adjustment part 1751 thereon.
The bottom of turning warehouse is provided with the first rotating part, and it includes the first receiving chamber 1243, and in order to accommodate turnable basement 122, first accommodates chamber 1243 is arranged on the centre position of bottom；Accommodating both sides, chamber first is symmetrically arranged first lug 1244, and the centre of the first lug 1244 is provided with through hole 1246；In order to be connected with described turnable basement 122.Turnable basement rotates within first accommodates chamber 1243 relative to turning warehouse, when turning warehouse turns to horizontal level, operates reaction chip assembly；There is higher stability, ensure that the transmission of reagent and data is unimpeded simultaneously.
Refer to shown in Fig. 5 a-5c, reaction chip assembly is installed in the inner side of the centre of described turning warehouse, accordingly, is provided with the first cavity 1785 accommodating reaction chip assembly in the centre of turning warehouse；The bottom of the first cavity 1785 is provided with the first boss 1784, first boss 1784 is in order to carry described reaction chip assembly, first boss 1784 is provided with four guide post installing holes 1786, reaction chip assembly, through the two ends of turning warehouse, reaction chip assembly, is connected by guide post installing hole 1786 with turning warehouse；Be provided with the second boss 1783 in the upper end of the first described boss 1784, the second boss 1784 is in order to positioning supporting part；The second described boss 1784 upper end is provided with locating surface 1782, locating surface 1782 is provided with locking screw nail 1787.Reaction chip assembly described in above-mentioned first cavity body structure location, and, reaction chip assembly is when normally working, it is impossible to enough rotations, is merely able to move along guide post.
The upper surface in the first receiving chamber 1243 of described turning warehouse is provided with Reagent Tube installing hole 1788 and electric wire installing hole, Reagent Tube installing hole 1788 is provided with pipe clamp 03, through having Reagent Tube 02 and electric wire 01 in pipe clamp 03.
The dead bolt installing hole of the dead bolt 1252 that the sidepiece at described turning warehouse is additionally provided with in electronic lock assembly 125；Under the effect of locking member, turning warehouse is fixed together with door frame 121.
Incorporated by reference to shown in Fig. 6 a and 6b, the electronic lock assembly 125 of the present invention includes electronics lock and inductive switch 1251, described inductive switch is arranged on electronics lock, electronics lock is arranged on the sidepiece of turning warehouse, in embodiments of the present invention, electronics lock includes sidepiece installing plate 1843, offers installing hole 1844 on sidepiece installing plate 1483；Electronic lock is made to be fixed on turning warehouse by installing hole 1844 and installation screw.It is provided with the first framework 1841 and the second framework 1842 of symmetry in described sidepiece installing plate 1483 side, leaves the space installing inductive switch 1251 in the centre of the first framework and the second framework.
In embodiments of the present invention, described inductive switch 1251 includes the framework 1255 being arranged on outside, and the one side of framework 1255 is provided with connection screwed hole 1256, and it is fixed by attachment screw 1254 with described electronics lock；Described attachment screw 1254 is by the lug 1258 on side and first framework the 1841, second framework 1842, in order to increase the bonding strength of electronics lock.Described inductive switch 1251 is also associated with control circuit 1252, in order to inductive switch 1251 to be controlled.
Referring to shown in Fig. 6 c, described electronic lock assembly 125 is arranged on turning warehouse and the sidepiece of door frame 121, and dead bolt 1253 enters in the dead bolt hole 1247 being arranged on turning warehouse sidepiece after stretching out, it is achieved locking；When dead bolt 1253 is under the effect of electricity irritation, when retracting in dead bolt hole 1247, described turning warehouse can be rotated.
It is provided with pressing piece 1241 in the upper end in the outside of described turning warehouse, only described turning warehouse can be made to rotate by pressing piece 1241 pressing setting.Refer to shown in Figure 12, in embodiments of the present invention, described pressing piece 1241 is provided with opening 1730 for one end, one end is provided with the structure of hook, described hook is connected with the upper end-stopping plate of door frame, when pressing piece 1241 is by downward active force, hook departs from door frame baffle plate, and turning reaction warehouse body can rotate relative to door frame.
In embodiments of the present invention, described pressing piece 1241 is arranged in a positioning block 1762, the lower end of locating piece 1762 is provided with spring 1761, and spring is arranged in spring base 1763, and spring base 1763 is threadably mounted in the spring base hole 1781 of intermediate throughholes 1785 upper surface of turning reaction warehouse.
Incorporated by reference to shown in Fig. 5 b-5d, locating piece 1762 is arranged in the locating piece installation cavity 1791 of turning reaction warehouse upper end, is provided with positioning step 1790 in cavity.Locating piece 1762 in the embodiment of the present invention includes through hole 1794 and lower through-hole 1797, and one of both is by described pressing piece 1241；Locating and machining hole 1796 and installing hole 1795 are set in the side of locating piece.The lower end of the through hole installing pressing piece 1241 is provided with spring eye, and spring is contacted by the bottom in this hole with pressing piece 1241.
The embodiment of the present invention arranges electronic lock assembly and pressing piece, and turning reaction warehouse assembly and door frame are carried out twice locking, is respectively adopted automatically controlled and frame for movement mode, it is ensured that reaction chip runs in security context, it is to avoid maloperation.
Refer to shown in Fig. 2 and 7, turnable basement 122 in the embodiment of the present invention is the connection base with two the second lugs 1221, second lug 1221 is symmetricly set on a side 1223 of base, through hole 1224 it is provided with in the middle of it, it is co-axially mounted with the through hole 1246 arranged in the middle of the first lug 1244 on described turning warehouse, further, the second lug 1221 is arranged on the inner side of the first lug 1244.Turnable basement 122 is the intermediate connector arranged, and turning warehouse realizes upset by this turning connection seat 122 and positions, easy to operate.
Incorporated by reference to shown in Fig. 2, in embodiments of the present invention, door frame 121 has the structure of sidepiece baffle plate 1211 for one side, and it is one-body molded, in the both sides of described turnable basement 122 are arranged on sidepiece baffle plate 1211 and contacts location with sidepiece baffle plate 1211.
The first described lug 1224 is after being co-axially mounted with the second lug 1221, end and the upper-end contact of turnable basement 122, and, the sidepiece of turning warehouse contacts with sidepiece baffle plate 1211 medial surface of door frame 121, it is ensured that turning warehouse and turnable basement 122 are located just in sidepiece baffle plate 1211.
Door frame 121 is additionally provided with the receiving hole passed through for reaction chip assembly.The use of door frame, is not only able to so that the Stability Analysis of Structures of reaction chip assembly, and, also make turning warehouse and turnable basement compact conformation, it is simple to operation；CCD camera is more accurate with the location of reaction chip.
The first installing hole 1210 it is provided with in the bottom of door frame 121, fixing in order to be connected with motion platform assembly 14；The second installing hole being connected with turnable basement sidepiece it is additionally provided with in the middle part of door frame.
nullIncorporated by reference to shown in Fig. 7，In embodiments of the present invention，In order to save structure space and ensure stablizing of reagent delivery，The through hole 1246 arranged in the middle of the first described lug 1244 and the through hole of the second lug 1221 are provided with Reagent Tube the first fixation kit，It includes the pipe joint 1265 being arranged in the lug of left side、First packing ring 1264、First rotary shaft 1261，And it is arranged on the Reagent Tube joint 1266 in the lug of right side、Second packing ring 1267、Second rotary shaft 1269，Wherein，The first described packing ring 1264 and the second packing ring 1267 are arranged between turning warehouse and turnable basement，In the present embodiment，The diameter of the through hole 1246 arranged in the middle of the first described lug 1244 is less than the diameter of the through hole of the second lug 1221，Medial end at both forms the location rib to packing ring location，After installing two packing rings，Inside diameter of gasket is just identical with the diameter of the through hole of the second lug 1221.Location rib and packing ring are set in embodiments of the present invention, ensure gapless between turning warehouse and turnable basement, both good stabilities after combining, will not rock because there is gap, and, packing ring adds both contacts area so that stress between the two reduces, extension device service life.
The inner side of through hole 1246 and the inner side of packing ring that arrange in the middle of the first lug 1244 are provided with the first rotary shaft 1261 and the second rotary shaft 1269, and the structure of two rotary shafts is identical, is the quill shaft with positioning convex ring；Positioning convex ring is set in one end of rotary shaft, is provided with connection screwed hole, fixing in order to be connected with the lug connection portion 1263 of described turning warehouse, accordingly, it is provided with the positioning recess positioning described positioning convex ring in the end of described through hole 1246.Being recess in the centre at the both ends of rotary shaft 1269, inner side recess is in order to install location with described pipe joint 1265, Reagent Tube joint 1266.Being respectively mounted live wire and Reagent Tube 02 in pipe joint 1265, Reagent Tube joint 1266, it respectively leads in reaction chip assembly.Owing to the embodiment of the present invention arranging the assembly that can mutually overturn, when upset, for avoiding pipe fitting is impacted, Reagent Tube is installed in through-holes, substantially increases the security performance of Reagent Tube.
Referring to shown in Fig. 8, in embodiments of the present invention, described reaction chip assembly is arranged on the inner side of described turning warehouse.It is provided with the observation portion 1724 contacted with CCD camera in the inner side of reaction chip assembly, is provided with transparent baffle plate, in order to course of reaction is taken pictures.
Referring to shown in Fig. 9 a, the reaction chip assembly of the present invention includes reaction chip mounting seat 127, reaction warehouse base assembly 126 and the shading positioning component 128 reaction warehouse base assembly 126 and reaction chip mounting seat 127 locked, open；In order to take out reaction chip easily and Guan Bi, reaction chip mounting seat 127 can rotate against with reaction warehouse base assembly 126.
Referring to shown in Fig. 9 b, it is the structural representation of reaction chip mounting seat of the present invention, and the mid portion in reaction chip mounting seat 127 is provided with reaction chip, is provided with cover plate 1277 on reaction chip；In the both sides of reaction chip, chute pressing plate 1279 is installed, in order to reaction chip to be fixed.A side portion in reaction chip mounting seat 127 is provided with mounting seat and props up support component, and it is in order to taking out or during chip placement, the reaction chip mounting seat described in support；It includes torsional-spring supported portion 1273, it is arranged in mounting seat, in order to support described torsion spring, described torsional-spring supported portion 1273 is one with the recess of protruding end 1274, when the reaction chip mounting seat 127 described in rotating, protruding end 1274 contacts with 1261 at first, and in the present embodiment, described protruding end 1274 is arranged on the middle part of recess and the edge of chip installation position；Reaction chip mounting seat is supported by the structure using mounting seat itself, better reliability, it is ensured that chip can safely remove and place.
Described mounting seat is propped up support component and is also included that torsion spring 1272 is connected with torsion spring 1272 and is arranged on the tension axle 1271 at torsion spring two ends, described tension axle 1271 is arranged in lobe 1275, when starting reaction chip mounting seat, described torsion spring 1272 supports mounting seat, and finally rely on the location of protruding end 1274, it is achieved support.
The first rotary shaft hole 1276 it is additionally provided with, in order to be connected with reaction warehouse base 1261 in the end of described lobe 1275.Sidepiece in reaction chip mounting seat is additionally provided with locating slot 1278.
Refer to shown in Fig. 9 c, it is the structural representation of medial surface of reaction warehouse base of the present invention, reaction cell body 1266 it is provided with in the medial surface of reaction warehouse, at reaction cell body 1266 mid portion, reaction tank 1267 is set, surrounding 1706 at reaction tank 1267 is provided with sealing ring, prevents reaction reagent from leaking.One end of reaction warehouse base 1261 is provided with raised line 1705, in order to install after cover plate location.The other end is provided with the torsional-spring supported structure corresponding with reaction chip mounting seat, and it includes symmetrically arranged two protruding ends 1268, is provided with the second rotary shaft hole 1269 thereon, and it is coaxial with the first rotary shaft hole 1276, and rotary shaft sequentially passes through holes；It is additionally provided with the region 1260 movable for torsion spring and region 1707, location in the end of reaction warehouse base 1261 other end, is provided with in this region in order to the screwed hole 1712 by installing reaction warehouse cover plate.
Refer to shown in Fig. 9 d, it is the structural representation of lateral surface of reaction warehouse base of the present invention, mid portion at the lateral surface of reaction warehouse base is provided with temperature control assembly, mid portion at reaction warehouse base is provided with temperature sensor mounting groove 1262, it is provided with heating wire mounting groove 1704 in the bottom of mounting groove 1262, places heating wire and temperature sensor respectively.In mounting groove, it is additionally provided with a positioning boss 1703, positions when it is in order to install with temperature control assembly cover plate 1263.As shown in figure 9e, temperature control assembly cover plate 1263 is provided with the positioning recess 1702 corresponding with positioning boss 1703.
Surrounding at temperature sensor mounting groove 1262 is provided with four guide post installing holes 1742, and upper and lower sides is provided with self adaptation spring mounting hole 1741；Cover plate 1263 is also equipped with guide rod aperture 1701.
Reaction warehouse base is additionally provided with shading positioning component mounting groove 1265, in order to install described shading positioning component 128, is within it provided with installation base 1744.
Refer to shown in Figure 10 a, it is the structural representation of shading positioning component of the present invention, it includes slidably positioning and locking block 1281 and turnable basement assembly 1283, wherein, it is provided with locking jaw 1282 on described slidably positioning and locking block 1281, after installation is complete, the described upper surface of slidably positioning and locking block 1281 contacts with the medial surface of reaction chip mounting seat 127, and locking jaw 1282 is hooked on the outboard end of reaction chip mounting seat 127；Described turnable basement assembly 1283 is arranged on the medial end of reaction warehouse base, is provided with screwed hole 1830.After installation is complete, described shading positioning component 128 is fixed together with reaction warehouse base, when opening reaction chip mounting seat, outwards pressing slidably positioning and locking block 1281, under the action of the spring, slidably positioning and locking block 1281 is outward-dipping, locking jaw more than 1282 reaction warehouse mounting seat departs from, under the effect of the torsion spring of reaction warehouse mounting seat end, reaction warehouse mounting seat overturns, and can be replaced reaction chip.
Incorporated by reference to shown in Figure 10 b, described slidably positioning and locking block 1281 includes the elastic locking assembly of the pressing plate 1823 and bottom homonymy being arranged on top, joint face 1821 convex under the mid portion of pressing plate 1823 is, it coordinates with described turnable basement assembly 1283.Incorporated by reference to shown in Figure 10 c, the first recess 1827 that described elastic locking assembly includes symmetrically arranged first spring base the 1822, second spring base 1828, is arranged between two spring bases；Being respectively arranged with spring assembly in two spring bases, spring assembly includes that the upper spring card slot 1281 shown in Figure 10 e, lower spring draw-in groove 1283, upper and lower two spring card slots surround spring eye 1771, and spring eye is shoulder hole, is provided with spring 1820 in it；Wherein, upper spring card slot 1281 is provided with external part, it arranges hook claw, in order to be connected with the end of described pressing plate 1823.
In embodiments of the present invention, spring base and the first recess are formed by continuous print vertical baffle 1826 isolation, and both sides arrange vertical rib 1829；Elastic locking assembly is arranged on the first flat board 1825 integrated with pressing plate, and it is vertically arranged with pressing plate 1823.
Referring to shown in Figure 10 d, the slidably positioning and locking block 1281 in the embodiment of the present invention can symmetrical coordinate with turnable basement assembly 1283, is provided with the first projection 1832 in the centre of turnable basement, and it snaps together with the first recess 1827；The space that can accommodate vertical baffle 1826 it is provided with in the first projection 1832 both sides；Accordingly, being provided with the second recess 1833 of spring base described in receiving in the first projection 1832 lateral symmetry, the second recess is surrounded by continuous print vertical edge 1834；Said structure is arranged on integrated second flat board 1831；In use, spring assembly is pressed against in spring base, slip positioning and locking block 1281 and turnable basement assembly 1283 are snapped together.The embodiment of the present invention, arranges shading positioning component, is not only able to stable locking reaction chip, and when changing reaction chip, simple to operate, only need to press slip positioning and locking block 1281 and open, and without dismantling reaction chip mounting seat, save the time.
Referring to, shown in Figure 11 a and 11b, the middle part of reaction warehouse base be provided with electrical heating wire 1754, it is arranged in the heating wire mounting groove 1704 of base；In the temperature sensor mounting groove 1262 on electrical heating wire top, it is provided with temperature sensor 1720 and controls assembly, in order to detect the temperature of reaction tank and to be adjusted according to the threshold value set；Also watering be inoculated with sealing member 1263 in temperature sensor 1720 and control assembly 1753, it is encapsulating resin.
It is respectively arranged with Reagent Tube at the two ends of reaction tank and screwed hole 1721 is installed, in order to be connected with Reagent Tube；The end installing screwed hole 1721 at Reagent Tube is provided with inlet 1723 and liquid outlet 1713, and liquid inlet and outlet communicates with the two ends of reaction tank, in order to cycle detection reagent.
Avris in the interior grooves of reaction tank is provided with sealing ring 1713.Being provided with reaction chip 04 in the centre of reaction chip mounting seat, reagent flows through reaction chip and reacts.
In embodiments of the present invention, the Reagent Tube 02 Reagent Tube installing hole 1788 by turning warehouse, the centre being arranged on turning warehouse is provided with the first cavity 1785 accommodating reagent pipeline, final coupled reaction cell body, reagent cartridge is through the sealing of various cavitys, and stability is high, is hardly damaged.
Rear side at reaction warehouse is fixed with back shroud 1242, the centre of back shroud 1242 is provided with self-adaptative adjustment part 1751, refer to shown in Figure 13 a-13c, in the middle of the inner side of back shroud 1242, it is provided with two rows raised line 1809 is installed, the centre installing raised line 1809 is provided with the first installation base 1812 and the second installation base 1811, offering screwed hole 1802 on installation base, self-adaptative adjustment part 1751 is arranged in this screwed hole 1802.
In embodiments of the present invention, described self-adaptative adjustment part 1751 is the end of thread with the connection end of back shroud, is the polished rod 1805 being provided with spring 1806 with the end that connects of reaction warehouse assembly, is provided with nut 1804 between the ends and positions, after installation is complete, nut 1804 is close to installation base.
It is passage 1803 between two described raised lines 1809, it is possible to Reagent Tube is passed through；Back shroud is additionally provided with the reinforcement gusset 1812 vertical with raised line 1809；Upper surface 1810 bit plane at raised line 1809 with boss；Below, centre and the corner of the peripheral framework 1807 of plate are provided with trip bolt hole 1801, fixing in order to be connected with described turning reaction warehouse.
Above-mentioned back shroud assembly, is not only able to reaction warehouse assembly is carried out face seal, additionally it is possible to install fixing self-adaptative adjustment part, simple in construction.
In embodiments of the present invention, it is provided with self-adaptative adjustment part 1751 between turning reaction warehouse and reaction warehouse base 126, refers to shown in Figure 12, the end spring of self-adaptative adjustment part 1751 and the end contact of reaction warehouse base；When using CCD camera feeding, when camera and reaction chip component touch, when adjusting camera position, can adjust in the way of employing is movable, now, self-adaptative adjustment part can change the position of reaction chip assembly according to the change in location of camera, it is ensured that has higher accuracy of detection, simultaneously, it is to avoid system is caused damage.
In embodiments of the present invention, in order to ensure that turning reaction warehouse assembly can coaxially move with reaction chip assembly, convenient adjustment, it is additionally provided with guide post 1758 between the two, refer to shown in Figure 11 c, arranging guiding rod aperture 1742 on reaction warehouse base, arrange guide post installing hole 1786 on turning warehouse, guide post 1742 sequentially passes through holes.Described guide post 1758 includes reaction warehouse locating flange 1757, in order to position with guiding rod aperture 1742；Positioned by the end face of nut 1755 with guide post installing hole 1786 at the other end, between be additionally provided with pad 1756.
In embodiments of the present invention, when described reaction warehouse assembly 12 works, above-mentioned reaction chip assembly is arranged in turning reaction warehouse, installs guide post 1758 and self-adaptative adjustment part 1751, and packaged back shroud；After turnable basement assembly 122 installation, lock turning reaction warehouse and door frame by electronic lock assembly and pressing piece, and the reaction warehouse assembly 12 of installation is arranged on platform assembly 13.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, and this embodiment is also not used to limit the scope of the claims of the present invention, and all equivalences done without departing from the present invention are implemented or change, are intended to be limited solely by the range of technical solution of the present invention.
Claims (5)
1. the erecting device of a DNA sequencer reaction chip, it is characterized in that, reaction chip is arranged in reaction warehouse assembly, described reaction warehouse assembly includes turning warehouse, in the inner side of the centre of described turning warehouse, reaction chip assembly is installed, accordingly, the centre at turning warehouse is provided with the first cavity accommodating reaction chip assembly；
Be provided with the first boss in the bottom of the first cavity, the first boss is in order to carry described reaction chip assembly；Be provided with the second boss in the upper end of the first described boss, the second boss is in order to positioning supporting part；The second described boss upper end is provided with locating surface, locating surface is provided with locking screw nail；
Described reaction chip assembly includes reaction chip mounting seat, reaction warehouse base assembly and the shading positioning component that reaction warehouse base assembly and reaction chip mounting seat are locked, opened；Mid portion in reaction chip mounting seat is provided with reaction chip, is provided with cover plate on reaction chip；In the both sides of reaction chip, chute pressing plate is installed；
In the medial surface of reaction warehouse, it is provided with reaction cell body, reaction tank is set at reaction cell body mid portion；Mid portion at the lateral surface of reaction warehouse base is provided with temperature control assembly, and the mid portion at reaction warehouse base is provided with temperature sensor mounting groove；
It is respectively arranged with Reagent Tube at the two ends of reaction tank and screwed hole is installed, in order to be connected with Reagent Tube；The end installing screwed hole at Reagent Tube is provided with inlet and liquid outlet, and liquid inlet and outlet communicates with the two ends of reaction tank；
Avris in the interior grooves of reaction tank is provided with sealing ring, is provided with reaction chip in the centre of reaction chip mounting seat, and reagent flows through reaction chip and reacts.
The erecting device of DNA sequencer reaction chip the most according to claim 1, it is characterized in that, a side portion in described reaction chip mounting seat is provided with mounting seat and props up support component, and it is in order to taking out or during chip placement, the reaction chip mounting seat described in support；
Described mounting seat is propped up support component and is included torsional-spring supported portion, is arranged in mounting seat, and described torsional-spring supported portion is one with the recess of protruding end, and when the reaction chip mounting seat described in rotating, protruding end contacts with reaction warehouse base assembly at first.
The erecting device of DNA sequencer reaction chip the most according to claim 2, it is characterized in that, described mounting seat is propped up support component and is also included that torsion spring is connected with torsion spring and is arranged on the tension axle at torsion spring two ends, described tension axle is arranged in lobe, when starting reaction chip mounting seat, described torsional-spring supported mounting seat, and finally rely on the location of protruding end；
It is additionally provided with the first rotary shaft hole, in order to be connected with reaction warehouse base in the end of described lobe；Sidepiece in reaction chip mounting seat is additionally provided with locating slot.
The erecting device of DNA sequencer reaction chip the most according to claim 2, it is characterised in that one end of described reaction warehouse base is provided with raised line, in order to install after cover plate location；
The other end is provided with the torsional-spring supported structure corresponding with reaction chip mounting seat, and it includes symmetrically arranged two protruding ends, is provided with the second rotary shaft hole thereon, and it is coaxial with the first rotary shaft hole, and rotary shaft sequentially passes through holes；It is additionally provided with the region movable for torsion spring and region, location in the end of the reaction warehouse base other end, is provided with in this region in order to the screwed hole by installing reaction warehouse cover plate.
The erecting device of DNA sequencer reaction chip the most according to claim 2, it is characterised in that be provided with Reagent Tube installing hole and electric wire installing hole on the upper surface in the first receiving chamber of described turning warehouse, be provided with pipe clamp in Reagent Tube installing hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716204.XA CN105273992B (en) | 2015-10-29 | 2015-10-29 | A kind of erecting device of DNA sequencer reaction chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716204.XA CN105273992B (en) | 2015-10-29 | 2015-10-29 | A kind of erecting device of DNA sequencer reaction chip |
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| CN105273992A CN105273992A (en) | 2016-01-27 |
| CN105273992B true CN105273992B (en) | 2016-09-14 |
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| CN201510716204.XA Expired - Fee Related CN105273992B (en) | 2015-10-29 | 2015-10-29 | A kind of erecting device of DNA sequencer reaction chip |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107655892B (en) * | 2017-10-31 | 2023-09-12 | 安徽能通新能源科技有限公司 | Lithium battery monitoring device |
| CN113174307B (en) * | 2021-01-15 | 2022-02-15 | 北京中科生仪科技有限公司 | Upper cover based on nucleic acid detection chip installation bin |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102703311B (en) * | 2012-05-24 | 2013-08-21 | 中国科学院北京基因组研究所 | Reaction bin for adaptive regulation of DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703312B (en) * | 2012-05-24 | 2014-03-19 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703302B (en) * | 2012-05-24 | 2013-11-27 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
| CN102703314B (en) * | 2012-05-24 | 2013-09-04 | 中国科学院北京基因组研究所 | Control system for DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102766574B (en) * | 2012-05-24 | 2013-12-25 | 中国科学院北京基因组研究所 | Reaction chamber for DNA sequenator |
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