CN105273993B - A kind of DNA sequencer reaction warehouse system - Google Patents
A kind of DNA sequencer reaction warehouse system Download PDFInfo
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- CN105273993B CN105273993B CN201510716482.5A CN201510716482A CN105273993B CN 105273993 B CN105273993 B CN 105273993B CN 201510716482 A CN201510716482 A CN 201510716482A CN 105273993 B CN105273993 B CN 105273993B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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Abstract
The present invention relates to a kind of DNA sequencer reaction warehouse system, including the door frame being vertically arranged, the top in the outside of described door frame is provided with turning reaction warehouse assembly；Connect the middle part having turnable basement, turnable basement to be fixed on door frame in the lower end of turning reaction warehouse assembly, turning reaction warehouse assembly can rotate around described turnable basement, in order to take out or to put into reaction chip；In the inner side of the centre of described turning warehouse, reaction chip assembly is installed, in reaction chip inner assembly, is provided with reaction chip, react with the reagent for sequenator；The first cavity accommodating reaction chip assembly it is provided with, in order to position described reaction chip assembly in the centre of turning warehouse.The DNA sequencer reaction warehouse system of the present invention has the robust structure of many recanalization, easy for installation between each structure, compact conformation, and stability is high.
Description
Technical field
The present invention relates to DNA sequencing field, particularly relate to a kind of DNA sequencer reaction warehouse system.
Background technology
DNA sequencing operating process of the prior art is described as follows: after DNA sample is by crushing, and application is built storehouse reagent and entered
Row adds joint, strand capture, is bound to microsphere, microemulsion PCR amplification, breakdown of emulsion liquid, it is thus achieved that set up the DNA library on microsphere,
Application adds model by laies such as the enzymes of library and sequencing reaction needs to having the sequence testing chip of micro reaction pool, sequence testing chip and survey
Sequence reagent is installed to main frame, starts order-checking program by controlling computer according to module number and position, and automatization surveys
Sequence is reacted, and the data of generation are transmitted to Data Analysis Computer, after completing order-checking computation analyze software carry out image procossing,
The work such as sequence reading, quality analysis, sequence assembly, finally give the sequence information of DNA sample.It is carved with the order-checking of micro reaction pool
Chip is carried out machine glazing core layer etching by the fibre faceplate of core diameter 25 μ m thick 2mm and obtains, etching depth 40 μm, on chip altogether
Count about 3,000,000 micro reaction pools, wherein about 1,200,000 micro reaction pools of imaging moiety.Micro reaction pool sequence testing chip is sequencing reaction
Carrier, the DNA Beads and the various sequencing reaction enzyme that are loaded with sequencing template are respectively positioned on the sequence testing chip being carved with micro reaction pool
In.
In sequencing procedure, sequence testing chip occurs chemical reaction, produces visible ray, by CCD (Charge Couple
Device) optical signal produced by cameras capture sequencing reaction, i.e. available required order-checking information.In measuring technology,
Reaction warehouse, as the main carriers of test reaction, should have enough stability and operability, it is possible to according to actual anti-
Answer situation, convenient adjustment.
In view of drawbacks described above, creator of the present invention obtains this creation finally through research for a long time and practice.
Summary of the invention
It is an object of the invention to provide a kind of DNA sequencer reaction warehouse system, in order to overcome above-mentioned technological deficiency.
For achieving the above object, the present invention provides a kind of DNA sequencer reaction warehouse system, including the door frame being vertically arranged,
The top in the outside of described door frame is provided with turning reaction warehouse assembly；
The middle part having turnable basement, turnable basement to be fixed on door frame is connected in the lower end of turning reaction warehouse assembly,
Turning reaction warehouse assembly can rotate around described turnable basement, in order to take out or to put into reaction chip；
In the inner side of the centre of described turning warehouse, reaction chip assembly is installed, arranges in reaction chip inner assembly
Respond chip, react with the reagent for sequenator；
The first cavity accommodating reaction chip assembly it is provided with, in order to position described reaction in the centre of turning warehouse
Chip assembly.
Further, described door frame two sides there is sidepiece baffle plate, the both sides of described turnable basement set
Put in sidepiece baffle plate and contact location with sidepiece baffle plate；
Described door frame is additionally provided with the receiving hole passed through for reaction chip assembly, itself and described turning warehouse
On the first cavity communicate；
The bottom of described door frame is provided with the first installing hole, and in order to be connected with motion platform assembly, fixed dna checks order
Instrument reaction warehouse system.
Further, being provided with the first rotating part in the bottom of described turning warehouse, the first rotating part includes first
Accommodating chamber, in order to accommodate turnable basement, first accommodates chamber is arranged on the centre position of bottom；
Accommodating both sides, chamber first is symmetrically arranged first lug, and the centre of the first lug is provided with through hole；In order to
It is connected with described turnable basement, rotates in order to make turnable basement accommodate intracavity relative to turning warehouse first, can turn over
When roll-over body turns to horizontal level, reaction chip assembly is operated；
The first described lug after being co-axially mounted with the second lug, the upper-end contact of end and turnable basement.
Further, the mid portion at described turning warehouse is provided with back shroud, in order to encapsulate Reagent Tube, and leads to
Cross self-adaptative adjustment part thereon and adjust reaction warehouse assembly and the position of turning warehouse；
Described self-adaptative adjustment part includes spring, spring end and reaction warehouse component touch, reaction warehouse assembly compression bullet
Spring, adjusts self-position.
Further, described self-adaptative adjustment part is arranged on the centre position, inner side of back shroud, at described back shroud
Inner side in the middle of be provided with two rows raised line be installed, be provided with the first installation base and second in the centre installing raised line and install convex
Platform, offers screwed hole on installation base, and self-adaptative adjustment part is arranged in this screwed hole；
Described self-adaptative adjustment part is the end of thread with the connection end of back shroud, is setting with the connection end of reaction warehouse assembly
There is the polished rod of spring, be provided with nut between the ends and position.
Further, being provided with the first boss in the bottom of the first described cavity, the first boss is described in order to carry
Reaction chip assembly；Being provided with four guide post installing holes on the first boss, guide post installing hole is through turning warehouse
Two ends, reaction chip assembly, be connected reaction chip assembly with turning warehouse；
Be provided with the second boss in the upper end of the first described boss, the second boss is in order to position described support member；?
The second described boss upper end is provided with locating surface, is provided with locking screw nail on locating surface.
Further, the upper end in the outside of described upset warehouse being provided with pressing piece, described pressing piece is one end
Be provided with opening, one end is provided with the structure of hook, and described hook is connected with the upper end-stopping plate of door frame, pressing piece by
Under active force time, hook and door frame baffle plate depart from, and turning reaction warehouse body can rotate relative to door frame；
Described pressing piece is arranged in a positioning block, and the lower end of locating piece is provided with spring, and spring is arranged on spring base
In, in the spring base hole of the intermediate throughholes upper surface that spring base is threadably mounted at turning reaction warehouse.
Further, described locating piece is arranged in the locating piece installation cavity of turning reaction warehouse upper end, at cavity
Inside it is provided with positioning step；Described locating piece includes through hole and lower through-hole, and one of both is by described pressing piece；
The side of described locating piece arranges locating and machining hole and installing hole；Set in the lower end of the through hole installing pressing piece
Being equipped with spring eye, spring is contacted with the bottom of pressing piece by this hole.
Further, described turnable basement is the connection base with two the second lugs, the second described lug
It is symmetricly set on a side of base, in the middle of it, is provided with through hole, with the first lug on described turning warehouse
The through hole arranged in the middle of is co-axially mounted, and, the second described lug is arranged on the inner side of the first lug.
Further, described door frame sidepiece is provided with electronic lock assembly, its turning reaction warehouse group described in locking
Part, under the effect of locking member, turning warehouse is fixed together with door frame；
Described electronic lock assembly includes that electronics lock and inductive switch, described inductive switch are arranged on electronics lock
On, described electronics lock is arranged on the sidepiece of turning warehouse, and electronics lock includes that sidepiece installing plate is on sidepiece installing plate
Offer installing hole；Electronic lock is made to be fixed on turning warehouse by installing hole and installation screw.
Compared with prior art the invention have the benefit that the DNA sequencing reaction storehouse assembly of the present invention has many readjustments
Whole robust structure, easy for installation between each structure, compact conformation, stability is high.Reaction warehouse assembly can be installed, fixes and adjust
Whole described reaction chip, it is ensured that reaction chip reacts in stable environment, and, reaction chip can conveniently take out
And locking.
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of the DNA sequencer reacting record subsystem of the present invention；
Fig. 2 is the first perspective view of the reaction warehouse assembly of the present invention；
Fig. 3 is the second perspective view of the reaction warehouse assembly of the present invention；
Fig. 4 be the reaction warehouse assembly of the present invention face structural representation；
Fig. 5 a is the perspective view of the turning reaction warehouse assembly of the present invention；
Fig. 5 b is the first perspective view of the turning warehouse of the present invention；
Fig. 5 c is the second perspective view of the turning warehouse of the present invention；
Fig. 5 d is the first perspective view of the locating piece of the present invention；
Fig. 5 e is the second perspective view of the locating piece of the present invention；
Fig. 6 a is the structural representation of the electronic lock of the present invention；
Fig. 6 b is the structural representation of the electronics lock of the present invention；
Fig. 6 c is the sectional structure schematic diagram of the electronic lock of the present invention；
Fig. 7 is the turning reaction warehouse assembly sectional structure schematic diagram along the first lug of the present invention；
Fig. 8 is the 3rd perspective view of the turning warehouse of the present invention；
Fig. 9 a is the structural representation of the reaction chip assembly of the present invention；
Fig. 9 b is the structural representation of the reaction chip mounting seat of the present invention；
Fig. 9 c is the structural representation of the medial surface of the reaction warehouse base of the present invention；
Fig. 9 d is the structural representation of the lateral surface of the reaction warehouse base of the present invention；
Fig. 9 e is the structural representation of the temperature control assembly cover plate of the present invention；
Figure 10 a is the structural representation of the shading positioning component of the present invention；
Figure 10 b is the first structural representation of the slidably positioning and locking block of the present invention；
Figure 10 c is the second structural representation of the slidably positioning and locking block of the present invention；
Figure 10 d is the structural representation of the turnable basement assembly of the present invention；
Figure 10 e is the structural representation of the spring assembly of the present invention；
Figure 11 a is the sectional structure schematic diagram along liquid inlet and outlet of the reaction chip assembly of the present invention；
Figure 11 b is the turning reaction warehouse assembly sectional structure schematic diagram along self-adaptative adjustment part of the present invention；
Figure 11 c is the turning reaction warehouse assembly sectional structure schematic diagram along guide post of the present invention；
Figure 12 is that the turning reaction warehouse assembly of present invention A-A along Fig. 4 is to sectional structure schematic diagram；
Figure 13 a is the sectional structure schematic diagram of the back shroud of the present invention；
Figure 13 b is the perspective view of the back shroud assembly of the present invention；
Figure 13 c is the perspective view of the back shroud of the present invention；
Figure 14 a is the structural representation of the sealing ring of the present invention；
Figure 14 b is the sealing ring sectional structure schematic diagram of the present invention；
Figure 14 c is the use view that the present invention observes the sealing ring of right side reaction chip.
Detailed description of the invention
Below in conjunction with accompanying drawing, to the present invention, above-mentioned and other technical characteristic and advantage are described in more detail.
Referring to shown in Fig. 1, it is the overall structure schematic diagram of DNA sequencer reacting record subsystem of the present invention, this
Reacting record subsystem in inventive embodiments include reaction warehouse assembly 12, motion platform assembly 14, motion control unit 15,
CCD camera 101 and platform assembly 13, wherein,
Described reaction warehouse assembly 12, arranges reaction chip assembly in it, under the effect of control system, reactant liquor enters
Reaction chip carries out chemical reaction, produces visible ray；Described motion platform assembly 14, CCD camera is fed into suitably by it
Picture-taking position, the visible ray information produced in reaction chip is taken pictures collection；Described platform assembly 13, its
Reaction warehouse assembly 12 described in support, motion platform assembly 14；Described motion control unit, it is to CCD camera and reaction core
The operation of sheet controls in real time.
In embodiments of the present invention, described reaction warehouse assembly 12 can install, fixing and reaction chip described in adjusting,
Ensure that reaction chip reacts in stable environment, and, reaction chip can conveniently take out and lock.
Referring to shown in Fig. 2-4, described reaction warehouse assembly 12 includes being arranged on described platform assembly 13
The door frame 121 being vertically arranged, is provided with turning reaction warehouse assembly 124, in turning reaction on the top in the outside of door frame 121
The lower end of storehouse assembly 124 connects has turnable basement 122, turnable basement 122 to be fixed on the middle part of door frame 121, turning instead
Answer storehouse assembly 124 can to rotate around described turnable basement 122, in order to take out or to put into reaction chip.
It is additionally provided with electronic lock assembly 125, only at electronic lock assembly 125 in the left side of the upper end of described door frame 121
When opening, turning reaction warehouse assembly 124 can be rotated, reaction chip is operated.In embodiments of the present invention, if
It is equipped with door frame 121, supports reaction chip and dependency structure, substantially increase the stability of system.
Turning reaction warehouse assembly 124 includes turning warehouse, and the mid portion at described turning warehouse is provided with
Back shroud 1242, in order to encapsulate Reagent Tube, and adjusts reaction warehouse assembly and turning by self-adaptative adjustment part 1751 thereon
The position of warehouse.
The bottom of turning warehouse is provided with the first rotating part, and it includes the first receiving chamber 1243, turning in order to accommodate
Base 122, first accommodates chamber 1243 is arranged on the centre position of bottom；It is symmetrically arranged first convex for accommodating both sides, chamber first
Ear 1244, is provided with through hole 1246 in the centre of the first lug 1244；In order to be connected with described turnable basement 122.Can turn over
Turn base to rotate, when turning warehouse turns to horizontal level, to reaction within first accommodates chamber 1243 relative to turning warehouse
Chip assembly operates；There is higher stability, ensure that the transmission of reagent and data is unimpeded simultaneously.
Refer to shown in Fig. 5 a-5c, in the inner side of the centre of described turning warehouse, reaction chip assembly, phase are installed
Answer, be provided with the first cavity 1785 accommodating reaction chip assembly in the centre of turning warehouse；At the first cavity 1785
Bottom is provided with the first boss 1784, and the first boss 1784 is in order to carry described reaction chip assembly, at the first boss 1784
Being provided with four guide post installing holes 1786, guide post installing hole 1786 passes the two ends of turning warehouse, reaction chip assembly,
Reaction chip assembly is connected with turning warehouse；The upper end of the first described boss 1784 is provided with the second boss 1783,
Second boss 1784 is in order to position described support member；The second described boss 1784 upper end is provided with locating surface 1782,
Locking screw nail 1787 it is provided with on locating surface 1782.Reaction chip assembly described in above-mentioned first cavity body structure location, and,
Reaction chip assembly is when normally working, it is impossible to enough rotations, is merely able to move along guide post.
The upper surface in the first receiving chamber 1243 of described turning warehouse is provided with Reagent Tube installing hole 1788 He
Electric wire installing hole, is provided with pipe clamp 03 in Reagent Tube installing hole 1788, through having Reagent Tube 02 and electric wire 01 in pipe clamp 03.
The dead bolt of the dead bolt 1252 that the sidepiece at described turning warehouse is additionally provided with in electronic lock assembly 125 is installed
Hole；Under the effect of locking member, turning warehouse is fixed together with door frame 121.
Incorporated by reference to shown in Fig. 6 a and 6b, the electronic lock assembly 125 of the present invention includes electronics lock and inductive switch 1251, institute
The inductive switch stated is arranged on electronics lock, and electronics lock is arranged on the sidepiece of turning warehouse, in embodiments of the present invention,
Electronics lock includes sidepiece installing plate 1843, offers installing hole 1844 on sidepiece installing plate 1483；Electronic lock is made by peace
Dress hole 1844 and installation screw are fixed on turning warehouse.Symmetry it is provided with in described sidepiece installing plate 1483 side
First framework 1841 and the second framework 1842, leaves the sky installing inductive switch 1251 in the centre of the first framework and the second framework
Between.
In embodiments of the present invention, described inductive switch 1251 includes the framework 1255 being arranged on outside, framework 1255
One side on be provided with connection screwed hole 1256, it is fixed by attachment screw 1254 with described electronics lock；Described
Attachment screw 1254 is by the lug 1258 on side and first framework the 1841, second framework 1842, in order to increase electronics lock
Bonding strength.Described inductive switch 1251 is also associated with control circuit 1252, in order to inductive switch 1251 to be carried out
Control.
Referring to shown in Fig. 6 c, described electronic lock assembly 125 is arranged on turning warehouse and the sidepiece of door frame 121, lock
Tongue 1253 enters in the dead bolt hole 1247 being arranged on turning warehouse sidepiece after stretching out, and realizes locking；When dead bolt 1253 stings at electricity
Under the effect swashed, when retracting in dead bolt hole 1247, described turning warehouse can be rotated.
It is provided with pressing piece 1241 in the upper end in the outside of described turning warehouse, is only being pressed by pressing piece 1241
Pressure arranges and described turning warehouse can be made to rotate.Refer to shown in Figure 12, in embodiments of the present invention, described pressing
Part 1241 is provided with opening 1730 for one end, one end is provided with the structure of hook, and the upper end-stopping plate of described hook and door frame is even
Connecing, when pressing piece 1241 is by downward active force, hook departs from door frame baffle plate, and turning reaction warehouse body can phase opposite house
Frame rotates.
In embodiments of the present invention, described pressing piece 1241 is arranged in a positioning block 1762, locating piece 1762 times
End is provided with spring 1761, and spring is arranged in spring base 1763, and spring base 1763 is threadably mounted at turning reaction warehouse
In the spring base hole 1781 of intermediate throughholes 1785 upper surface.
Incorporated by reference to shown in Fig. 5 b-5d, locating piece 1762 is arranged on the locating piece installation cavity of turning reaction warehouse upper end
In 1791, in cavity, it is provided with positioning step 1790.Locating piece 1762 in the embodiment of the present invention includes through hole 1794 He
Lower through-hole 1797, one of both is by described pressing piece 1241；Arrange in the side of locating piece locating and machining hole 1796 and
Installing hole 1795.The lower end of the through hole installing pressing piece 1241 is provided with spring eye, and spring is by this hole and pressing piece 1241
Bottom contact.
The embodiment of the present invention arranges electronic lock assembly and pressing piece, and turning reaction warehouse assembly and door frame are carried out two
Secondary locking, is respectively adopted automatically controlled and frame for movement mode, it is ensured that reaction chip runs in security context, it is to avoid maloperation.
Referring to shown in Fig. 2 and 7, the turnable basement 122 in the embodiment of the present invention is for having two the second lugs 1221
Connection base, the second lug 1221 is symmetricly set on a side 1223 of base, is provided with through hole 1224 in the middle of it,
It is co-axially mounted with the through hole 1246 arranged in the middle of the first lug 1244 on described turning warehouse, and, the second lug
1221 inner sides being arranged on the first lug 1244.Turnable basement 122 is the intermediate connector arranged, and turning warehouse is by being somebody's turn to do
Turning connection seat 122 realizes upset and positions, easy to operate.
Incorporated by reference to shown in Fig. 2, in embodiments of the present invention, door frame 121 has the structure of sidepiece baffle plate 1211 for one side,
It is one-body molded, in the both sides of described turnable basement 122 are arranged on sidepiece baffle plate 1211 and contacts with sidepiece baffle plate 1211
Location.
The first described lug 1224 is after being co-axially mounted with the second lug 1221, and end is upper with turnable basement 122
End in contact, and, the sidepiece of turning warehouse contacts with sidepiece baffle plate 1211 medial surface of door frame 121, it is ensured that turning warehouse
It is located just in sidepiece baffle plate 1211 with turnable basement 122.
Door frame 121 is additionally provided with the receiving hole passed through for reaction chip assembly.The use of door frame, be not only able to so that
The Stability Analysis of Structures of reaction chip assembly, and, also make turning warehouse and turnable basement compact conformation, it is simple to operation；CCD
Camera is more accurate with the location of reaction chip.
The first installing hole 1210 it is provided with in the bottom of door frame 121, fixing in order to be connected with motion platform assembly 14；At door
The second installing hole being connected with turnable basement sidepiece it is additionally provided with in the middle part of frame.
Incorporated by reference to shown in Fig. 7, in embodiments of the present invention, in order to save structure space and ensure the steady of reagent delivery
Fixed, the through hole 1246 arranged in the middle of the first described lug 1244 and the through hole of the second lug 1221 are provided with reagent
Pipe the first fixation kit, it includes pipe joint the 1265, first packing ring the 1264, first rotary shaft being arranged in the lug of left side
1261, and it is arranged on Reagent Tube joint the 1266, second packing ring the 1267, second rotary shaft 1269 in the lug of right side, wherein, institute
The first packing ring 1264 stated and the second packing ring 1267 are arranged between turning warehouse and turnable basement, in the present embodiment,
The diameter of the through hole 1246 arranged in the middle of the first described lug 1244 is less than the diameter of the through hole of the second lug 1221,
Both medial ends form the location rib to packing ring location, and after installing two packing rings, inside diameter of gasket is just with second
The diameter of the through hole of lug 1221 is identical.Location rib and packing ring are set in embodiments of the present invention, it is ensured that turning storehouse
Gapless between body and turnable basement, both good stabilities after combining, will not rock because there is gap, and,
Packing ring adds both contacts area so that stress between the two reduces, extension device service life.
The inner side of through hole 1246 and the inner side of packing ring that arrange in the middle of the first lug 1244 are provided with the first rotation
Axle 1261 and the second rotary shaft 1269, the structure of two rotary shafts is identical, is the quill shaft with positioning convex ring；In rotary shaft
One end positioning convex ring is set, be provided with connection screwed hole, in order to the lug connection portion with described turning warehouse
1263 connections are fixing, accordingly, are provided with the positioning recess positioning described positioning convex ring in the end of described through hole 1246.?
The centre at the both ends of rotary shaft 1269 is recess, inner side recess in order to described pipe joint 1265, Reagent Tube joint
1266 install location.Being respectively mounted live wire and Reagent Tube 02 in pipe joint 1265, Reagent Tube joint 1266, it is respectively
Lead in reaction chip assembly.Owing to the embodiment of the present invention arranging the assembly that can mutually overturn, when upset, for avoiding pipe
Part impacts, and installs Reagent Tube in through-holes, substantially increases the security performance of Reagent Tube.
Referring to shown in Fig. 8, in embodiments of the present invention, described reaction chip assembly is arranged on described turning storehouse
The inner side of body.It is provided with the observation portion 1724 contacted with CCD camera in the inner side of reaction chip assembly, is provided with transparent
Baffle plate, in order to course of reaction is taken pictures.
Referring to shown in Fig. 9 a, the reaction chip assembly of the present invention includes reaction chip mounting seat 127, reaction warehouse base group
Part 126 and the shading positioning component 128 reaction warehouse base assembly 126 and reaction chip mounting seat 127 locked, opens；For
Can take out reaction chip easily and Guan Bi, reaction chip mounting seat 127 can be relative with reaction warehouse base assembly 126
Rotate.
Referring to shown in Fig. 9 b, it is the structural representation of reaction chip mounting seat of the present invention, installs at reaction chip
The mid portion of seat 127 is provided with reaction chip, is provided with cover plate 1277 on reaction chip；Install in the both sides of reaction chip
There is chute pressing plate 1279, in order to reaction chip to be fixed.A side portion in reaction chip mounting seat 127 is provided with installation
Seat supports assembly, it is in order to taking out or during chip placement, the reaction chip mounting seat described in support；It includes torsional-spring supported portion
1273, it is arranged in mounting seat, in order to support described torsion spring, described torsional-spring supported portion 1273 is one with protruding end 1274
Recess, rotate described in reaction chip mounting seat 127 time, protruding end 1274 contacts with 1261 at first, in the present embodiment,
Described protruding end 1274 is arranged on the middle part of recess and the edge of chip installation position；Use the structure of mounting seat itself
Reaction chip mounting seat is supported, better reliability, it is ensured that chip can safely remove and place.
Described mounting seat is propped up support component and is also included that torsion spring 1272 is connected with torsion spring 1272 and is arranged on drawing of torsion spring two ends
Mandrel 1271, described tension axle 1271 is arranged in lobe 1275, when starting reaction chip mounting seat, described torsion spring
1272 support mounting seat, and finally rely on the location of protruding end 1274, it is achieved support.
Be additionally provided with the first rotary shaft hole 1276 in the end of described lobe 1275, in order to reaction warehouse base 1261
Connect.Sidepiece in reaction chip mounting seat is additionally provided with locating slot 1278.
Referring to shown in Fig. 9 c, it is the structural representation of medial surface of reaction warehouse base of the present invention, at reaction warehouse
Medial surface is provided with reaction cell body 1266, at reaction cell body 1266 mid portion, reaction tank 1267 is set, at reaction tank 1267
Surrounding 1706 be provided with sealing ring, prevent reaction reagent from leaking.One end of reaction warehouse base 1261 is provided with raised line 1705,
In order to install after cover plate location.The other end is provided with the torsional-spring supported structure corresponding with reaction chip mounting seat, its
Including symmetrically arranged two protruding ends 1268, it is provided with the second rotary shaft hole 1269 thereon, itself and the first rotary shaft hole
1276 is coaxial, and rotary shaft sequentially passes through holes；The region 1260 movable for torsion spring and region, location it is additionally provided with in this end
1707, it is provided with in this region in order to the screwed hole 1712 by installing reaction warehouse cover plate.
Referring to shown in Fig. 9 d, it is the structural representation of lateral surface of reaction warehouse base of the present invention, at the bottom of reaction warehouse
The mid portion of the lateral surface of seat is provided with temperature control assembly, and the mid portion at reaction warehouse base is provided with temperature sensor and installs
Groove 1262, is provided with heating wire mounting groove 1704, places heating wire and temperature sensor respectively in the bottom of mounting groove 1262.
In mounting groove, it is additionally provided with a positioning boss 1703, positions when it is in order to install with temperature control assembly cover plate 1263.Such as Fig. 9 e institute
Show, temperature control assembly cover plate 1263 is provided with the positioning recess 1702 corresponding with positioning boss 1703.
Surrounding at temperature sensor mounting groove 1262 is provided with four guide post installing holes 1742, is provided with at upper and lower sides
Self adaptation spring mounting hole 1741；Cover plate 1263 is also equipped with guide rod aperture 1701.
Reaction warehouse base is additionally provided with shading positioning component mounting groove 1265, in order to install described shading location group
Part 128, is within it provided with installation base 1744.
Referring to shown in Figure 10 a, it is the structural representation of shading positioning component of the present invention, and it includes slidably positioning
Latch segment 1281 and turnable basement assembly 1283, wherein, described slidably positioning and locking block 1281 is provided with locking jaw
1282, after installation is complete, the upper surface of described slidably positioning and locking block 1281 and the inner side of reaction chip mounting seat 127
Face contacts, and locking jaw 1282 is hooked on the outboard end of reaction chip mounting seat 127；Described turnable basement assembly 1283 is pacified
It is contained in the medial end of reaction warehouse base, is provided with screwed hole 1830.After installation is complete, described shading positioning component
128 are fixed together with reaction warehouse base, when opening reaction chip mounting seat, outwards press slidably positioning and locking block
1281, under the action of the spring, slidably positioning and locking block 1281 is outward-dipping, and locking jaw more than 1282 reaction warehouse mounting seat takes off
From, under the effect of the torsion spring of reaction warehouse mounting seat end, reaction warehouse mounting seat overturns, and can be replaced reaction chip.
Incorporated by reference to shown in Figure 10 b, described slidably positioning and locking block 1281 includes the pressing plate 1823 being arranged on top
And the elastic locking assembly of bottom homonymy, joint face 1821 convex under the mid portion of pressing plate 1823 is, it is with described
Turnable basement assembly 1283 coordinate.Incorporated by reference to shown in Figure 10 c, described elastic locking assembly includes symmetrically arranged first
Spring base the 1822, second spring base 1828, the first recess 1827 being arranged between two spring bases；Divide in two spring bases
Not being provided with spring assembly, spring assembly includes the upper spring card slot 1281 shown in Figure 10 e, lower spring draw-in groove 1283, upper and lower two
Individual spring card slot surrounds spring eye 1771, and spring eye is shoulder hole, is provided with spring 1820 in it；Wherein, at upper spring card slot
It is provided with external part on 1281, it arranges hook claw, in order to be connected with the end of described pressing plate 1823.
In embodiments of the present invention, spring base and the first recess are formed by continuous print vertical baffle 1826 isolation, and two
Side arranges vertical rib 1829；Elastic locking assembly is arranged on the first flat board 1825 integrated with pressing plate, its with press
Pressing plate 1823 is vertically arranged.
Refer to shown in Figure 10 d, the slidably positioning and locking block 1281 in the embodiment of the present invention and turnable basement assembly
1283 can symmetrical coordinate, and are provided with the first projection 1832 in the centre of turnable basement, and it is fastened on the first recess 1827
Together；The space that can accommodate vertical baffle 1826 it is provided with in the first projection 1832 both sides；Accordingly, the first projection 1832 liang
Side is symmetrically arranged with the second recess 1833 of the spring base described in receiving, and the second recess is surrounded by continuous print vertical edge 1834；
Said structure is arranged on integrated second flat board 1831；In use, spring assembly is pressed against in spring base, will be sliding
Dynamic positioning and locking block 1281 snaps together with turnable basement assembly 1283.The embodiment of the present invention, arranges shading location
Assembly, is not only able to stable locking reaction chip, and when changing reaction chip, simple to operate, only need to press slip fixed
Position locking block 1281 is opened, and without dismantling reaction chip mounting seat, saves the time.
Referring to, shown in Figure 11 a and 11b, the middle part of reaction warehouse base be provided with electrical heating wire 1754, it is arranged
In the heating wire mounting groove 1704 of base；In the temperature sensor mounting groove 1262 on electrical heating wire top, it is provided with temperature pass
Sensor 1720 and control assembly, in order to detect the temperature of reaction tank and to be adjusted according to the threshold value set；In temperature sensor
1720 and control assembly 1753 and also water and be inoculated with sealing member 1263, it is encapsulating resin.
It is respectively arranged with Reagent Tube at the two ends of reaction tank and screwed hole 1721 is installed, in order to be connected with Reagent Tube；At reagent
The end of pipe installation screwed hole 1721 is provided with the two ends of inlet 1723 and liquid outlet 1713, liquid inlet and outlet and reaction tank
Communicate, in order to cycle detection reagent.
Avris in the interior grooves of reaction tank is provided with sealing ring 1713.It is provided with in the centre of reaction chip mounting seat
Reaction chip 04, reagent flows through reaction chip and reacts.
In embodiments of the present invention, the Reagent Tube 02 Reagent Tube installing hole 1788 by turning warehouse, it is arranged on and can turn over
The centre of roll-over body is provided with the first cavity 1785 accommodating reagent pipeline, final coupled reaction cell body, and reagent cartridge is through each
Planting the sealing of cavity, stability is high, is hardly damaged.
Rear side at reaction warehouse is fixed with back shroud 1242, and the centre of back shroud 1242 is provided with self-adaptative adjustment part
1751, refer to shown in Figure 13 a-13c, in the middle of the inner side of back shroud 1242, be provided with two rows raised line 1809 is installed, installing
The centre of raised line 1809 is provided with the first installation base 1812 and the second installation base 1811, offers screw thread on installation base
Hole 1802, self-adaptative adjustment part 1751 is arranged in this screwed hole 1802.
In embodiments of the present invention, described self-adaptative adjustment part 1751 is the end of thread with the connection end of back shroud, and instead
The end that connects answering storehouse assembly is the polished rod 1805 being provided with spring 1806, is provided with nut 1804 between the ends and positions,
After installation is complete, nut 1804 is close to installation base.
It is passage 1803 between two described raised lines 1809, it is possible to Reagent Tube is passed through；Back shroud also sets
It is equipped with the reinforcement gusset 1812 vertical with raised line 1809；Upper surface 1810 bit plane at raised line 1809 with boss；At plate below
The centre of peripheral framework 1807 and corner be provided with trip bolt hole 1801, in order to described turning reaction warehouse even
Connect fixing.
Above-mentioned back shroud assembly, is not only able to reaction warehouse assembly is carried out face seal, additionally it is possible to installs fixing self adaptation and adjusts
One integral piece, simple in construction.
In embodiments of the present invention, it is provided with self-adaptative adjustment part between turning reaction warehouse and reaction warehouse base 126
1751, refer to shown in Figure 12, the end spring of self-adaptative adjustment part 1751 and the end contact of reaction warehouse base；Using
During CCD camera feeding, when camera and reaction chip component touch, when adjusting camera position, movable side can be used
Formula adjusts, and now, self-adaptative adjustment part can change the position of reaction chip assembly according to the change in location of camera, it is ensured that has
Higher accuracy of detection, simultaneously, it is to avoid system is caused damage.
In embodiments of the present invention, in order to ensure that turning reaction warehouse assembly can coaxially move with reaction chip assembly,
Convenient adjustment, is additionally provided with guide post 1758 between the two, refers to, shown in Figure 11 c, arrange guide post on reaction warehouse base
Hole 1742, arranges guide post installing hole 1786 on turning warehouse, and guide post 1742 sequentially passes through holes.Described guide post
1758 include reaction warehouse locating flange 1757, in order to position with guiding rod aperture 1742；At the other end by nut 1755 and guiding
Bar installing hole 1786 end face location, between be additionally provided with pad 1756.
In embodiments of the present invention, in order to test the diverse location of reaction chip, employing can change survey
The sealing ring 1713 of examination area；Referring to shown in Figure 14 a-14c, described sealing ring 1713 is hexagon, and it includes outer rim
1716 and be arranged on the first blend stop 1714 and the second blend stop 1715 of opposite side, the first blend stop 1714 and the second blend stop 1715 will examination
Agent is divided into three parts.In an embodiment, sidepiece rib and blend stop upper end be respectively first protruding 1718 and second
Protruding 1719, two projections make reagent flow in the range of limiting, and reagent liquid inlet and outlet is arranged between two blend stops
In chamfering.When not using blend stop, reagent flows into inside whole sealing ring by the chamfering between two blend stops, now, it is possible to
Observe the reaction of whole reaction chip；When state as shown in figures 14a, reaction chip between two blend stops can only be observed
Region；When state as shown in figure 14 c, can only region on the right side of observing response chip.
The embodiment of the present invention uses foldable sealing ring, substantially increases the motility of reaction test, can be as required
Reaction chip is sampled.
In embodiments of the present invention, when described reaction warehouse assembly 12 works, above-mentioned reaction chip assembly is arranged on can
In upset reaction warehouse, install guide post 1758 and self-adaptative adjustment part 1751, and packaged back shroud；With turnable basement
After assembly 122 installation, lock turning reaction warehouse and door frame by electronic lock assembly and pressing piece, and will peace
The reaction warehouse assembly 12 installed is arranged on platform assembly 13.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, and this embodiment is also not used to
Limiting the scope of the claims of the present invention, all equivalences done without departing from the present invention are implemented or change, are intended to be limited solely by the technology of the present invention
In the range of scheme.
Claims (8)
1. a DNA sequencer reaction warehouse system, it is characterised in that include the door frame being vertically arranged, the outside of described door frame
Top be provided with turning reaction warehouse assembly；
Connect the middle part having turnable basement, turnable basement to be fixed on door frame in the lower end of turning reaction warehouse assembly, can turn over
Turn reaction warehouse assembly can rotate around described turnable basement, in order to take out or to put into reaction chip；
In the inner side of the centre of described turning warehouse, reaction chip assembly is installed, is provided with anti-in reaction chip inner assembly
Answer chip, react with the reagent for sequenator；
The first cavity accommodating reaction chip assembly it is provided with, in order to position described reaction chip in the centre of turning warehouse
Assembly；
Described door frame two sides there is sidepiece baffle plate, the both sides of described turnable basement are arranged in sidepiece baffle plate
And contact location with sidepiece baffle plate；
Described door frame is additionally provided with the receiving hole passed through for reaction chip assembly, its with on described turning warehouse
First cavity communicates；
The bottom of described door frame is provided with the first installing hole, and in order to be connected with motion platform assembly, fixed dna sequenator is anti-
Answer storehouse system；
Be provided with the first rotating part in the bottom of described turning warehouse, the first rotating part includes the first receiving chamber, in order to hold
Receiving turnable basement, first accommodates chamber is arranged on the centre position of bottom；
Accommodating both sides, chamber first is symmetrically arranged first lug, and the centre of the first lug is provided with through hole；In order to institute
The turnable basement stated connects, and rotates in order to make turnable basement accommodate intracavity relative to turning warehouse first, turning storehouse
When body turns to horizontal level, reaction chip assembly is operated；
The first described lug after being co-axially mounted with the second lug, the upper-end contact of end and turnable basement.
DNA sequencer reaction warehouse system the most according to claim 1, it is characterised in that at described turning warehouse
Mid portion is provided with back shroud, in order to encapsulate Reagent Tube, and by self-adaptative adjustment part thereon adjust reaction warehouse assembly and
The position of turning warehouse；
Described self-adaptative adjustment part includes spring, spring end and reaction warehouse component touch, reaction warehouse assembly compression spring, adjusts
Whole self-position.
DNA sequencer reaction warehouse system the most according to claim 2, it is characterised in that described self-adaptative adjustment part sets
Put the centre position, inner side at back shroud, in the middle of the inner side of described back shroud, be provided with two rows raised line is installed, convex installing
The centre of bar is provided with the first installation base and the second installation base, offers screwed hole, self-adaptative adjustment on installation base
Part is arranged in this screwed hole；
The connection end of described self-adaptative adjustment part and back shroud is the end of thread, with the connection end of reaction warehouse assembly for being provided with bullet
The polished rod of spring, is provided with nut between the ends and positions.
DNA sequencer reaction warehouse system the most according to claim 1, it is characterised in that at the end of the first described cavity
Portion is provided with the first boss, and the first boss is in order to carry described reaction chip assembly；First boss is provided with four lead
To bar installing hole, guide post installing hole passes the two ends of turning warehouse, reaction chip assembly, by reaction chip assembly with can turn over
Roll-over body connects；
Be provided with the second boss in the upper end of the first described boss, the second boss is in order to position described reaction chip assembly；
The second described boss upper end is provided with locating surface, locating surface is provided with locking screw nail.
DNA sequencer reaction warehouse system the most according to claim 1, it is characterised in that outside described upset warehouse
The upper end of side is provided with pressing piece, and described pressing piece is that one end is provided with opening, one end is provided with the structure of hook, described
Hook is connected with the upper end-stopping plate of door frame, and when pressing piece is by downward active force, hook departs from door frame baffle plate, turning
Reaction warehouse body can rotate relative to door frame；
Described pressing piece is arranged in a positioning block, and the lower end of locating piece is provided with spring, and spring is arranged in spring base, bullet
In the spring base hole of the intermediate throughholes upper surface that spring abutment is threadably mounted at turning reaction warehouse.
DNA sequencer reaction warehouse system the most according to claim 5, it is characterised in that described locating piece is arranged on can
In the locating piece installation cavity of upset reaction warehouse upper end, in cavity, it is provided with positioning step；Described locating piece includes logical
Hole and lower through-hole, one of both is by described pressing piece；
The side of described locating piece arranges locating and machining hole and installing hole；It is provided with in the lower end of the through hole installing pressing piece
Spring eye, spring is contacted with the bottom of pressing piece by this hole.
DNA sequencer reaction warehouse system the most according to claim 1, it is characterised in that described turnable basement is tool
There are the connection base of two the second lugs, the second described lug to be symmetricly set on a side of base, arrange in the middle of it
Have through hole, with the first lug on described turning warehouse in the middle of the through hole that arranges be co-axially mounted, and, described the
Two lugs are arranged on the inner side of the first lug.
DNA sequencer reaction warehouse system the most according to claim 7, it is characterised in that arrange at described door frame sidepiece
Having electronic lock assembly, its turning reaction warehouse assembly described in locking, under the effect of locking member, turning warehouse is solid with door frame
It is scheduled on together；
Described electronic lock assembly includes that electronics lock and inductive switch, described inductive switch are arranged on electronics lock, institute
The electronics lock stated is arranged on the sidepiece of turning warehouse, and electronics lock includes that sidepiece installing plate offers on sidepiece installing plate
Installing hole；Electronic lock is made to be fixed on turning warehouse by installing hole and installation screw.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716482.5A CN105273993B (en) | 2015-10-29 | 2015-10-29 | A kind of DNA sequencer reaction warehouse system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716482.5A CN105273993B (en) | 2015-10-29 | 2015-10-29 | A kind of DNA sequencer reaction warehouse system |
Publications (2)
| Publication Number | Publication Date |
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| CN105273993A CN105273993A (en) | 2016-01-27 |
| CN105273993B true CN105273993B (en) | 2016-11-16 |
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| CN201510716482.5A Expired - Fee Related CN105273993B (en) | 2015-10-29 | 2015-10-29 | A kind of DNA sequencer reaction warehouse system |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723227A (en) * | 2016-08-12 | 2018-02-23 | 广州康昕瑞基因健康科技有限公司 | Gene sequencing reaction cell and gene sequencing reaction device |
| CN108265003A (en) * | 2016-12-30 | 2018-07-10 | 广州康昕瑞基因健康科技有限公司 | Multichannel gene sequencing reaction cell and multichannel gene sequencing reaction device |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703311B (en) * | 2012-05-24 | 2013-08-21 | 中国科学院北京基因组研究所 | Reaction bin for adaptive regulation of DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703312B (en) * | 2012-05-24 | 2014-03-19 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703302B (en) * | 2012-05-24 | 2013-11-27 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
| CN102766574B (en) * | 2012-05-24 | 2013-12-25 | 中国科学院北京基因组研究所 | Reaction chamber for DNA sequenator |
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| CN105273993A (en) | 2016-01-27 |
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