CN105273979B - A kind of DNA sequencer reacting record subsystem - Google Patents
A kind of DNA sequencer reacting record subsystem Download PDFInfo
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- CN105273979B CN105273979B CN201510716526.4A CN201510716526A CN105273979B CN 105273979 B CN105273979 B CN 105273979B CN 201510716526 A CN201510716526 A CN 201510716526A CN 105273979 B CN105273979 B CN 105273979B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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Abstract
The present invention relates to a kind of DNA sequencer reacting record subsystem, it includes reaction warehouse assembly, motion platform assembly, motion control unit, CCD camera and platform assembly, wherein, described reaction warehouse assembly, reaction chip assembly is set in it, under the effect of control system, reactant liquor enters and carries out chemical reaction in reaction chip, produces visible ray；Described motion platform assembly, CCD camera is fed into suitable picture-taking position by it, the collection so that the visible ray information produced in reaction chip is taken pictures by CCD camera；Described platform assembly, its support described in reaction warehouse assembly, motion platform assembly；Described motion control unit, the operation of CCD camera and reaction chip is controlled by real time, drives motion platform component movement to the picture-taking position of CCD camera, detects and control the temperature of reaction warehouse.The DNA sequencing reaction record subsystem of the present invention has the robust structure of many recanalization, easy for installation between each structure, compact conformation, and stability is high.
Description
Technical field
The present invention relates to DNA sequencing field, particularly relate to a kind of DNA sequencer reacting record subsystem.
Background technology
At DNA sequencing technical field, pyrosequencing techniques (pyrosequencing), it is in 1987 by Nyren et al.
A kind of novel enzyme cascade sequencing technologies grown up in year, its repeatable and accurate performance and Sanger method DNA sequencing skill
Art compares favourably, and speed is greatly improved.Pyrosequencing techniques is to be cascaded by the enzyme in 4 kinds of enzymatic same reaction systems
Chemiluminescence reaction.
The principle of pyrosequencing techniques is: after primer is annealed with template DNA, at archaeal dna polymerase (DNA
Polymerase), the double phosphorus of ATP sulfurylase (ATP sulfurylase), luciferase (luciferase) and adenosine triphosphate
Under the synergism of acid enzyme (Apyrase) four kinds of enzymes, by even to the polymerization of each dNTP on primer and the release of an optical signal
Connection gets up, and by release and the intensity of detection light, reaches the purpose of the real time measure DNA sequence.The reaction of pyrosequencing techniques
System is made up of reaction substrate, strand to be measured, sequencing primer and four kinds of enzymes.Reaction substrate is 5 '-phosphosulfate (adenosine-
5 '-phosphosulfate, APS) and fluorescein (luciferin).Take turns in sequencing reaction each, reaction system only adds
A kind of Deoxydization nucleotide triphosphoric acid (dNTP), if it then can just gather at DNA with the next base pairing of DNA profiling
Under the effect of synthase, add 3 ' ends of sequencing primer to, discharge the pyrophosphoric acid (PPi) of a molecule simultaneously.At ATP sulphuric acid
Changing under the effect of enzyme, the PPi of generation can combine with APS and form ATP, and under the catalysis of luciferase, the ATP of generation is the most permissible
Combine with fluorescein and form oxyluciferin, produce visible ray simultaneously.Can be obtained by Weak light detection device and process software
One special detection peak, the height of peak value is then directly proportional with the base number matched.After last round of reaction completes, add another
A kind of dNTP, makes above-mentioned reaction repeat, and the peak value figure according to obtaining i.e. can read DNA sequence information accurately.
Integrated operation flow process is described as follows: DNA sample is by after broken, and application builds that storehouse reagent carries out adding joint, strand is caught
Obtain, be bound to microsphere, microemulsion PCR amplification, breakdown of emulsion liquid, it is thus achieved that setting up the DNA library on microsphere, application adds model by library
The lay such as enzyme needed with sequencing reaction is to having the sequence testing chip of micro reaction pool, and sequence testing chip and sequencing reagent are installed to main frame
On, starting order-checking program by controlling computer according to module number and position, automatization carries out sequencing reaction, the data of generation
Transmission is to Data Analysis Computer, and after completing order-checking, computation analysis software carries out image procossing, sequence reads, quality is divided
The work such as analysis, sequence assembly, finally give the sequence information of DNA sample..Micro reaction pool sequence testing chip is the load of sequencing reaction
Body, the DNA Beads and the various sequencing reaction enzyme that are loaded with sequencing template are respectively positioned in the sequence testing chip being carved with micro reaction pool.
In sequencing procedure, sequence testing chip occurs chemical reaction, produces visible ray, by CCD (Charge Couple
Device) optical signal produced by cameras capture sequencing reaction, i.e. available required order-checking information.In sequenator, instead
The subsystem information to sequencing reaction should be recorded, it is desirable to reaction warehouse has the adjustable of enough precision and installation
Property, reaction warehouse, as the main carriers of test reaction, also should have enough stability.
In view of drawbacks described above, creator of the present invention obtains this creation finally through research for a long time and practice.
Summary of the invention
It is an object of the invention to provide a kind of DNA sequencer reacting record subsystem, in order to overcome above-mentioned technological deficiency.
For achieving the above object, the present invention provides a kind of DNA sequencer reacting record subsystem, and it includes reaction warehouse group
Part, motion platform assembly, motion control unit, CCD camera and platform assembly, wherein,
Described reaction warehouse assembly, arranges reaction chip assembly in it, under the effect of control system, reactant liquor enters anti-
Answer and chip carries out chemical reaction, produce visible ray；
Described motion platform assembly, CCD camera is fed into suitable picture-taking position by it, so that CCD camera is to reaction
The visible ray information produced in chip carries out collection of taking pictures；
Described platform assembly, its support described in reaction warehouse assembly, motion platform assembly；
Described motion control unit, the operation of CCD camera and reaction chip is controlled by real time, drives motion platform
Component movement, to the picture-taking position of CCD camera, detects and controls the temperature of reaction warehouse；
Described reaction warehouse assembly includes the vertical door frame being arranged on described platform assembly, outside door frame
The top of side is provided with turning reaction warehouse assembly, and connecting in the lower end of turning reaction warehouse assembly has turnable basement, can turn over
Turning base and be fixed on the middle part of door frame, turning reaction warehouse assembly can rotate around described turnable basement, in order to taking out or
Put into reaction chip；
Described turning reaction warehouse assembly includes turning warehouse, and the inner side of turning warehouse is provided with reaction chip
Assembly, is provided with reaction chip in reaction chip assembly, and reagent solution passes through reaction chip generation chemical reaction.
Further, described platform assembly includes vibration damper plate and is arranged on the first damping wing of vibration damper plate both sides
Plate and the second damping wing plate, be respectively arranged with dampening assembly in the lower end of two damping wing plates；
Platform assembly is indentations, and reaction warehouse assembly is arranged on vibration damper plate, and two ends connect with two vibration damper plates respectively
Touch.
Further, described damping wing plate includes gripper shoe, and it has certain height, the reaction warehouse group described in support
Part；
The centre of the integrated upper head plate of gripper shoe is provided with the mounting groove of motion control unit, is provided with motion in it
The circuit board of control unit and various circuit；
It is reinforcing plate in the lower end of mounting groove, in order to offer mounting groove.
Further, described motion platform assembly include lead screw guide rails assembly that motor is connected with motor output shaft,
Support support seat and detection components, the camera support assembly of lead screw assembly；
The output shaft of motor is connected by shaft coupling with leading screw, and motor and leading screw junction are provided with support seat, at leading screw
End be provided with bearing and axial positioning sleeve；
Connecting on leading screw has nut, nut to be arranged in nut mounting seat；The upper end of nut mounting seat is with connecting plate even
Connecing, the upper end of connecting plate connects camera mounting seat；At bearing end, bearing friction top is set；
Described nut mounting seat be arranged on on the slide block of guide rail contact, and on slide block drive CCD camera move.
Further, described detection components includes that detecting plate fixes seat, and it is arranged on the sidepiece of connecting plate, and detection plate is solid
The end of reservation connects has position to detect plate；Sidepiece at base is provided with position sensor mounting seat, pacifies at position sensor
The upper end of dress seat arranges contiguous block, and contiguous block upper end is fixed with position sensor；
Described sensor installation seat includes the screwed hole being arranged on side, is mounting groove in the centre of mounting seat, in order to
Installation site sensor；It is provided with slide recess between sidepiece baffle plate and bottom surface.
Further, the bottom of described turning warehouse being provided with the first rotating part, it includes the first receiving chamber, uses
To accommodate turnable basement, first accommodates chamber is arranged on the centre position of bottom；
Accommodating both sides, chamber first is symmetrically arranged first lug, and the centre of the first lug is provided with through hole；In order to
It is connected with described turnable basement.
Further, reaction chip assembly includes reaction chip mounting seat, reaction warehouse base assembly and by the bottom of reaction warehouse
The shading positioning component that holder assembly and reaction chip mounting seat are locked, opened；
Mid portion in reaction chip mounting seat is provided with reaction chip, and the side portion in reaction chip mounting seat sets
Being equipped with mounting seat and prop up support component, it is in order to taking out or during chip placement, the reaction chip mounting seat described in support.
Further, being additionally provided with electronic lock assembly in the left side of the upper end of described door frame, electronic lock assembly includes electricity
Sub-lock and inductive switch, described inductive switch is arranged on electronics lock, and electronics lock is arranged on the side of turning warehouse
Portion；
Sidepiece at described turning warehouse is provided with dead bolt installing hole；Dead bolt enters after stretching out and is arranged on turning storehouse
In the dead bolt hole in side portion.
Further, the middle part of reaction warehouse base being provided with electrical heating wire, it is arranged on the heating wire peace of base
In tankage；In the temperature sensor mounting groove on electrical heating wire top, it is provided with temperature sensor and controls assembly, in order to detect
The temperature of reaction tank is also adjusted according to the threshold value set；Also water be inoculated with sealing member in temperature sensor and control assembly, its
For encapsulating resin.
Further, being provided with self-adaptative adjustment part between turning reaction warehouse and reaction warehouse base, self adaptation is adjusted
The end spring of one integral piece and the end contact of reaction warehouse base；Self-adaptative adjustment part can change anti-according to the change in location of camera
Answer the position of chip assembly；
Turning reaction warehouse assembly coaxially moves with reaction chip assembly, is additionally provided with guide post between the two.
Compared with prior art the invention have the benefit that the DNA sequencing reaction record subsystem of the present invention has many
The robust structure of recanalization, easy for installation between each structure, compact conformation, stability is high.Reaction warehouse assembly can be installed, fix
And the reaction chip described in adjusting, it is ensured that reaction chip reacts in stable environment, and, reaction chip can facilitate
Take out and locking.
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of the DNA sequencer reacting record subsystem of the present invention；
Fig. 2 is the first perspective view of the reaction warehouse assembly of the present invention；
Fig. 3 is the second perspective view of the reaction warehouse assembly of the present invention；
Fig. 4 be the reaction warehouse assembly of the present invention face structural representation；
Fig. 5 a is the perspective view of the turning reaction warehouse assembly of the present invention；
Fig. 5 b is the first perspective view of the turning warehouse of the present invention；
Fig. 5 c is the second perspective view of the turning warehouse of the present invention；
Fig. 5 d is the first perspective view of the locating piece of the present invention；
Fig. 5 e is the second perspective view of the locating piece of the present invention；
Fig. 6 a is the structural representation of the electronic lock of the present invention；
Fig. 6 b is the structural representation of the electronics lock of the present invention；
Fig. 6 c is the sectional structure schematic diagram of the electronic lock of the present invention；
Fig. 7 is the turning reaction warehouse assembly sectional structure schematic diagram along the first lug of the present invention；
Fig. 8 is the 3rd perspective view of the turning warehouse of the present invention；
Fig. 9 a is the structural representation of the reaction chip assembly of the present invention；
Fig. 9 b is the structural representation of the reaction chip mounting seat of the present invention；
Fig. 9 c is the structural representation of the medial surface of the reaction warehouse base of the present invention；
Fig. 9 d is the structural representation of the lateral surface of the reaction warehouse base of the present invention；
Fig. 9 e is the structural representation of the temperature control assembly cover plate of the present invention；
Figure 10 a is the structural representation of the shading positioning component of the present invention；
Figure 10 b is the first structural representation of the slidably positioning and locking block of the present invention；
Figure 10 c is the second structural representation of the slidably positioning and locking block of the present invention；
Figure 10 d is the structural representation of the turnable basement assembly of the present invention；
Figure 10 e is the structural representation of the spring assembly of the present invention；
Figure 11 a is the sectional structure schematic diagram along liquid inlet and outlet of the reaction chip assembly of the present invention；
Figure 11 b is the turning reaction warehouse assembly sectional structure schematic diagram along self-adaptative adjustment part of the present invention；
Figure 11 c is the turning reaction warehouse assembly sectional structure schematic diagram along guide post of the present invention；
Figure 12 is that the turning reaction warehouse assembly of present invention A-A along Fig. 4 is to sectional structure schematic diagram；
Figure 13 a is the sectional structure schematic diagram of the back shroud of the present invention；
Figure 13 b is the perspective view of the back shroud assembly of the present invention；
Figure 13 c is the perspective view of the back shroud of the present invention；
Figure 14 a is the structural representation of the sealing ring of the present invention；
Figure 14 b is the sealing ring sectional structure schematic diagram of the present invention；
Figure 14 c is the use view that the present invention observes the sealing ring of right side reaction chip；
Figure 15 a is the structural representation of the platform assembly of the present invention；
Figure 15 b is the structural representation of the vibration damper plate of the present invention；
Figure 16 is the structural representation of the motion platform assembly of the present invention；
Figure 17 is the sectional structure schematic diagram of the motion platform assembly of the present invention；
Figure 18 a is the structural representation of the nut mounting seat of the present invention；
Figure 18 b is the motor structural representation supporting seat with leading screw junction of the present invention；
Figure 18 c is the structural representation of the detection components in the embodiment of the present invention；
Figure 18 d is the structural representation of position sensor mounting seat of the present invention.
Detailed description of the invention
Below in conjunction with accompanying drawing, to the present invention, above-mentioned and other technical characteristic and advantage are described in more detail.
Referring to shown in Fig. 1, it is the overall structure schematic diagram of DNA sequencer reacting record subsystem of the present invention, this
Reacting record subsystem in inventive embodiments include reaction warehouse assembly 12, motion platform assembly 14, motion control unit 15,
CCD camera 101 and platform assembly 13, wherein,
Described reaction warehouse assembly 12, arranges reaction chip assembly in it, under the effect of control system, reactant liquor enters
Reaction chip carries out chemical reaction, produces visible ray；Described motion platform assembly 14, CCD camera is fed into suitably by it
Picture-taking position, the visible ray information produced in reaction chip is taken pictures collection；Described platform assembly 13, its
Reaction warehouse assembly 12 described in support, motion platform assembly 14；Described motion control unit, it is to CCD camera and reaction core
The operation of sheet controls in real time.
In embodiments of the present invention, described reaction warehouse assembly 12 can install, fixing and reaction chip described in adjusting,
Ensure that reaction chip reacts in stable environment, and, reaction chip can conveniently take out and lock.
Referring to shown in Fig. 2-4, described reaction warehouse assembly 12 includes being arranged on described platform assembly 13
The door frame 121 being vertically arranged, is provided with turning reaction warehouse assembly 124, in turning reaction on the top in the outside of door frame 121
The lower end of storehouse assembly 124 connects has turnable basement 122, turnable basement 122 to be fixed on the middle part of door frame 121, turning instead
Answer storehouse assembly 124 can to rotate around described turnable basement 122, in order to take out or to put into reaction chip.
It is additionally provided with electronic lock assembly 125, only at electronic lock assembly 125 in the left side of the upper end of described door frame 121
When opening, turning reaction warehouse assembly 124 can be rotated, reaction chip is operated.In embodiments of the present invention, if
It is equipped with door frame 121, supports reaction chip and dependency structure, substantially increase the stability of system.
Turning reaction warehouse assembly 124 includes turning warehouse, and the mid portion at described turning warehouse is provided with
Back shroud 1242, in order to encapsulate Reagent Tube；Be provided with the first rotating part in bottom, it includes the first receiving chamber 1243, in order to hold
Receiving turnable basement 122, first accommodates chamber 1243 is arranged on the centre position of bottom；Both sides, chamber are accommodated for being symmetrical arranged first
The first lug 1244, the centre of the first lug 1244 is provided with through hole 1246；In order to described turnable basement 122 even
Connect.Turnable basement rotates within first accommodates chamber 1243 relative to turning warehouse, has higher stability, ensures examination simultaneously
The transmission of agent and data is unimpeded.
Refer to shown in Fig. 5 a-5c, in the inner side of the centre of described turning warehouse, reaction chip assembly, phase are installed
Answer, be provided with the first cavity 1785 accommodating reagent pipeline in the centre of turning warehouse, in the bottom of the first cavity 1785
Being provided with the first boss 1784, the first boss 1784 is in order to carry the support member of described reaction chip assembly, at the first boss
1784 are provided with four guide post installing holes 1786, and guide post installing hole 1786 is through the two ends of turning warehouse, reaction chip
Assembly and support member, be connected reaction chip assembly with turning warehouse；Arrange in the upper end of the first described boss 1784
Having the second boss 1783, the second boss 1784 is in order to position described support member；Arrange in the second described boss 1784 upper end
There is locating surface 1782, locating surface 1782 is provided with locking screw nail 1787.Above-mentioned first cavity body structure location described in anti-
Answer chip assembly, and, reaction chip assembly is when normally working, it is impossible to enough rotations, is merely able to move along guide post.
The upper surface in the first receiving chamber 1243 of described turning warehouse is provided with Reagent Tube installing hole 1788 He
Electric wire installing hole, is provided with pipe clamp 03 in Reagent Tube installing hole 1788, through having Reagent Tube 02 and electric wire 01 in pipe clamp 03.
The dead bolt of the dead bolt 1252 that the sidepiece at described turning warehouse is additionally provided with in electronic lock assembly 125 is installed
Hole；Under the effect of locking member, turning warehouse is fixed together with door frame 121.
Incorporated by reference to shown in Fig. 6 a and 6b, the electronic lock assembly 125 of the present invention includes electronics lock and inductive switch 1251, institute
The inductive switch stated is arranged on electronics lock, and electronics lock is arranged on the sidepiece of turning warehouse, in embodiments of the present invention,
Electronics lock includes sidepiece installing plate 1843, offers installing hole 1844 on sidepiece installing plate 1483；Electronic lock is made by peace
Dress hole 1844 and installation screw are fixed on turning warehouse.Symmetry it is provided with in described sidepiece installing plate 1483 side
First framework 1841 and the second framework 1842, leaves the sky installing inductive switch 1251 in the centre of the first framework and the second framework
Between.
In embodiments of the present invention, described inductive switch 1251 includes the framework 1255 being arranged on outside, framework 1255
One side on be provided with connection screwed hole 1256, it is fixed by attachment screw 1254 with described electronics lock；Described
Attachment screw 1254 is by the lug 1258 on side and first framework the 1841, second framework 1842, in order to increase electronics lock
Bonding strength.Described inductive switch 1251 is also associated with control circuit 1252, in order to inductive switch 1251 to be carried out
Control.
Referring to shown in Fig. 6 c, described electronic lock assembly 125 is arranged on turning warehouse and the sidepiece of door frame 121, lock
Tongue 1253 enters in the dead bolt hole 1247 being arranged on turning warehouse sidepiece after stretching out, and realizes locking；When dead bolt 1253 stings at electricity
Under the effect swashed, when retracting in dead bolt hole 1247, described turning warehouse can be rotated.
It is provided with pressing piece 1241 in the upper end in the outside of described turning warehouse, is only being pressed by pressing piece 1241
Pressure arranges and described turning warehouse can be made to rotate.Refer to shown in Figure 12, in embodiments of the present invention, described pressing
1241 one end of part are provided with opening 1730, one end is provided with the structure of hook, and the upper end-stopping plate of described hook and door frame is even
Connecing, when pressing piece 1241 is by downward active force, hook departs from door frame baffle plate, and turning reaction warehouse body can phase opposite house
Frame rotates.
In embodiments of the present invention, described pressing piece 1241 is arranged in a positioning block 1762, locating piece 1762 times
End is provided with spring 1761, and spring is arranged in spring base 1763, and spring base 1763 is threadably mounted at turning reaction warehouse
In the spring base hole 1781 of intermediate throughholes 1785 upper surface.
Incorporated by reference to shown in Fig. 5 b-5d, locating piece 1762 is arranged on the locating piece installation cavity of turning reaction warehouse upper end
In 1791, in cavity, it is provided with positioning step 1790.Locating piece 1762 in the embodiment of the present invention includes through hole 1794 He
Lower through-hole 1797, one of both is by described pressing piece 1241；Arrange in the side of locating piece locating and machining hole 1796 and
Installing hole 1795.The lower end of the through hole installing pressing piece 1241 is provided with spring eye, and spring is by this hole and pressing piece 1241
Bottom contact.
The embodiment of the present invention arranges electronic lock assembly and pressing piece, and turning reaction warehouse assembly and door frame are carried out two
Secondary locking, is respectively adopted automatically controlled and frame for movement mode, it is ensured that reaction chip runs in security context, it is to avoid maloperation.
Referring to shown in Fig. 2 and 7, the turnable basement in the embodiment of the present invention 122 has two the second lugs 1221
Connection base, the second lug 1221 is symmetricly set on a side 1223 of base, is provided with through hole 1224 in the middle of it,
It is co-axially mounted with the through hole 1246 arranged in the middle of the first lug 1244 on described turning warehouse, and, the second lug
1221 inner sides being arranged on the first lug 1244.
Incorporated by reference to shown in Fig. 2, in embodiments of the present invention, door frame 121 has the structure of sidepiece baffle plate 1211 for one side,
It is one-body molded, in the both sides of described turnable basement 122 are arranged on sidepiece baffle plate 1211 and contacts with sidepiece baffle plate 1211
Location；The first described lug 1224 after being co-axially mounted with the second lug 1221, the upper termination of end and turnable basement 122
Touch, and, the sidepiece of turning warehouse contacts with sidepiece baffle plate 1211 medial surface of door frame 121, it is ensured that turning warehouse with can
Upset base 122 is located just in sidepiece baffle plate 1211.
Door frame 121 is additionally provided with the receiving hole passed through for reaction chip assembly.The use of door frame, be not only able to so that
The Stability Analysis of Structures of reaction chip assembly, and, also make turning warehouse and turnable basement compact conformation, it is simple to operation；CCD
Camera is more accurate with the location of reaction chip.
The first installing hole 1210 it is provided with in the bottom of door frame 121, fixing in order to be connected with motion platform assembly 14；At door
The second installing hole being connected with turnable basement sidepiece it is additionally provided with in the middle part of frame.
Incorporated by reference to shown in Fig. 7, in embodiments of the present invention, in order to save structure space and ensure the steady of reagent delivery
Fixed, the through hole 1246 arranged in the middle of the first described lug 1244 and the through hole of the second lug 1221 are provided with reagent
Pipe the first fixation kit, it includes pipe joint the 1265, first packing ring the 1264, first rotary shaft being arranged in the lug of left side
1261, and it is arranged on Reagent Tube joint the 1266, second packing ring the 1267, second rotary shaft 1269 in the lug of right side, wherein, institute
The first packing ring 1264 stated and the second packing ring 1267 are arranged between turning warehouse and turnable basement, in the present embodiment,
The diameter of the through hole 1246 arranged in the middle of the first described lug 1244 is less than the diameter of the through hole of the second lug 1221,
Both medial ends form the location rib to packing ring location, and after installing two packing rings, inside diameter of gasket is just with second
The diameter of the through hole of lug 1221 is identical.Location rib and packing ring are set in embodiments of the present invention, it is ensured that turning storehouse
Gapless between body and turnable basement, both good stabilities after combining, will not rock because there is gap, and,
Packing ring adds both contacts area so that stress between the two reduces, extension device service life.
The inner side of through hole 1246 and the inner side of packing ring that arrange in the middle of the first lug 1244 are provided with the first rotation
Axle 1261 and the second rotary shaft 1269, the structure of two rotary shafts is identical, is the quill shaft with positioning convex ring；In rotary shaft
One end positioning convex ring is set, be provided with connection screwed hole, in order to the lug connection portion with described turning warehouse
1263 connections are fixing, accordingly, are provided with the positioning recess positioning described positioning convex ring in the end of described through hole 1246.?
The centre at the both ends of rotary shaft 1269 is recess, inner side recess in order to described pipe joint 1265, Reagent Tube joint
1266 install location.Being respectively mounted live wire and Reagent Tube 02 in pipe joint 1265, Reagent Tube joint 1266, it is respectively
Lead in reaction chip assembly.Owing to the embodiment of the present invention arranging the assembly that can mutually overturn, when upset, for avoiding pipe
Part impacts, and installs Reagent Tube in through-holes, substantially increases the security performance of Reagent Tube.
Referring to shown in Fig. 8, in embodiments of the present invention, described reaction chip assembly is arranged on described turning storehouse
The inner side of body.It is provided with the observation portion 1724 contacted with CCD camera in the inner side of reaction chip assembly, is provided with transparent
Baffle plate, in order to course of reaction is taken pictures.
Referring to shown in Fig. 9 a, the reaction chip assembly of the present invention includes reaction chip mounting seat 127, reaction warehouse base group
Part 126 and the shading positioning component 128 reaction warehouse base assembly 126 and reaction chip mounting seat 127 locked, opens；For
Can take out reaction chip easily and Guan Bi, reaction chip mounting seat 127 can be relative with reaction warehouse base assembly 126
Rotate.
Referring to shown in Fig. 9 b, it is the structural representation of reaction chip mounting seat of the present invention, installs at reaction chip
The mid portion of seat 127 is provided with reaction chip, is provided with cover plate 1277 on reaction chip；Install in the both sides of reaction chip
There is chute pressing plate 1279, in order to reaction chip to be fixed.A side portion in reaction chip mounting seat 127 is provided with installation
Seat supports assembly, it is in order to taking out or during chip placement, the reaction chip mounting seat described in support；It includes torsional-spring supported portion
1273, it is arranged in mounting seat, in order to support described torsion spring, described torsional-spring supported portion 1273 is one with protruding end 1274
Recess, rotate described in reaction chip mounting seat 127 time, protruding end 1274 contacts with 1261 at first, in the present embodiment,
Described protruding end 1274 is arranged on the middle part of recess and the edge of chip installation position；Use the structure of mounting seat itself
Reaction chip mounting seat is supported, better reliability, it is ensured that chip can safely remove and place.
Described mounting seat is propped up support component and is also included that torsion spring 1272 is connected with torsion spring 1272 and is arranged on drawing of torsion spring two ends
Mandrel 1271, described tension axle 1271 is arranged in lobe 1275, when starting reaction chip mounting seat, described torsion spring
1272 support mounting seat, and finally rely on the location of protruding end 1274, it is achieved support.
Be additionally provided with the first rotary shaft hole 1276 in the end of described lobe 1275, in order to reaction warehouse base 1261
Connect.Sidepiece in reaction chip mounting seat is additionally provided with locating slot 1278.
Referring to shown in Fig. 9 c, it is the structural representation of medial surface of reaction warehouse base of the present invention, at reaction warehouse
Medial surface is provided with reaction cell body 1266, at reaction cell body 1266 mid portion, reaction tank 1267 is set, at reaction tank 1267
Surrounding 1706 be provided with sealing ring, prevent reaction reagent from leaking.One end of reaction warehouse base 1261 is provided with raised line 1705,
In order to install after cover plate location.The other end is provided with the torsional-spring supported structure corresponding with reaction chip mounting seat, its
Including symmetrically arranged two protruding ends 1268, it is provided with the second rotary shaft hole 1269 thereon, itself and the first rotary shaft hole
1276 is coaxial, and rotary shaft sequentially passes through holes；The region 1260 movable for torsion spring and region, location it is additionally provided with in this end
1707, it is provided with in this region in order to the screwed hole 1712 by installing reaction warehouse cover plate.
Referring to shown in Fig. 9 d, it is the structural representation of lateral surface of reaction warehouse base of the present invention, at the bottom of reaction warehouse
The mid portion of the lateral surface of seat is provided with temperature control assembly, and the mid portion at reaction warehouse base is provided with temperature sensor and installs
Groove 1262, is provided with heating wire mounting groove 1704, places heating wire and temperature sensor respectively in the bottom of mounting groove 1262.
In mounting groove, it is additionally provided with a positioning boss 1703, positions when it is in order to install with temperature control assembly cover plate 1263.Such as Fig. 9 e institute
Show, temperature control assembly cover plate 1263 is provided with the positioning recess 1702 corresponding with positioning boss 1703.
Surrounding at temperature sensor mounting groove 1262 is provided with four guide post installing holes 1742, is provided with at upper and lower sides
Self adaptation spring mounting hole 1741；Cover plate 1263 is also equipped with guide rod aperture 1701.
Reaction warehouse base is additionally provided with shading positioning component mounting groove 1265, in order to install described shading location group
Part 128, is within it provided with installation base 1744.
Referring to shown in Figure 10 a, it is the structural representation of shading positioning component of the present invention, and it includes slidably positioning
Latch segment 1281 and turnable basement assembly 1283, wherein, described slidably positioning and locking block 1281 is provided with locking jaw
1282, after installation is complete, the upper surface of described slidably positioning and locking block 1281 and the inner side of reaction chip mounting seat 127
Face contacts, and locking jaw 1282 is hooked on the outboard end of reaction chip mounting seat 127；Described turnable basement assembly 1283 is pacified
It is contained in the medial end of reaction warehouse base, is provided with screwed hole 1830.After installation is complete, described shading positioning component
128 are fixed together with reaction warehouse base, when opening reaction chip mounting seat, outwards press slidably positioning and locking block
1281, under the action of the spring, slidably positioning and locking block 1281 is outward-dipping, and locking jaw more than 1282 reaction warehouse mounting seat takes off
From, under the effect of the torsion spring of reaction warehouse mounting seat end, reaction warehouse mounting seat overturns, and can be replaced reaction chip.
Incorporated by reference to shown in Figure 10 b, described slidably positioning and locking block 1281 includes the pressing plate 1823 being arranged on top
And the elastic locking assembly of bottom homonymy, joint face 1821 convex under the mid portion of pressing plate 1823 is, it is with described
Turnable basement assembly 1283 coordinate.Incorporated by reference to shown in Figure 10 c, described elastic locking assembly includes symmetrically arranged first
Spring base the 1822, second spring base 1828, the first recess 1827 being arranged between two spring bases；Divide in two spring bases
Not being provided with spring assembly, spring assembly includes the upper spring card slot 1281 shown in Figure 10 e, lower spring draw-in groove 1283, upper and lower two
Individual spring card slot surrounds spring eye 1771, and spring eye is shoulder hole, is provided with spring 1820 in it；Wherein, at upper spring card slot
It is provided with external part on 1281, it arranges hook claw, in order to be connected with the end of described pressing plate 1823.
In embodiments of the present invention, spring base and the first recess are formed by continuous print vertical baffle 1826 isolation, and two
Side arranges vertical rib 1829；Elastic locking assembly is arranged on the first flat board 1825 integrated with pressing plate, its with press
Pressing plate 1823 is vertically arranged.
Refer to shown in Figure 10 d, the slidably positioning and locking block 1281 in the embodiment of the present invention and turnable basement assembly
1283 can symmetrical coordinate, and are provided with the first projection 1832 in the centre of turnable basement, and it is fastened on the first recess 1827
Together；The space that can accommodate vertical baffle 1826 it is provided with in the first projection 1832 both sides；Accordingly, the first projection 1832 liang
Side is symmetrically arranged with the second recess 1833 of the spring base described in receiving, and the second recess is surrounded by continuous print vertical edge 1834；
Said structure is arranged on integrated second flat board 1831；In use, spring assembly is pressed against in spring base, will be sliding
Dynamic positioning and locking block 1281 snaps together with turnable basement assembly 1283.The embodiment of the present invention, arranges shading location
Assembly, is not only able to stable locking reaction chip, and when changing reaction chip, simple to operate, only need to press slip fixed
Position locking block 1281 is opened, and without dismantling reaction chip mounting seat, saves the time.
Referring to, shown in Figure 11 a and 11b, the middle part of reaction warehouse base be provided with electrical heating wire 1754, it is arranged
In the heating wire mounting groove 1704 of base；In the temperature sensor mounting groove 1262 on electrical heating wire top, it is provided with temperature pass
Sensor 1720 and control assembly, in order to detect the temperature of reaction tank and to be adjusted according to the threshold value set；In temperature sensor
1720 and control assembly 1753 and also water and be inoculated with sealing member 1263, it is encapsulating resin.
It is respectively arranged with Reagent Tube at the two ends of reaction tank and screwed hole 1721 is installed, in order to be connected with Reagent Tube；At reagent
The end of pipe installation screwed hole 1721 is provided with the two ends of inlet 1723 and liquid outlet 1713, liquid inlet and outlet and reaction tank
Communicate, in order to cycle detection reagent.
Avris in the interior grooves of reaction tank is provided with sealing ring 1713.It is provided with in the centre of reaction chip mounting seat
Reaction chip 04, reagent flows through reaction chip and reacts.
In embodiments of the present invention, the Reagent Tube 02 Reagent Tube installing hole 1788 by turning warehouse, it is arranged on and can turn over
The centre of roll-over body is provided with the first cavity 1785 accommodating reagent pipeline, final coupled reaction cell body, and reagent cartridge is through each
Planting the sealing of cavity, stability is high, is hardly damaged.
Rear side at reaction warehouse is fixed with back shroud 1242, and the centre of back shroud 1242 is provided with self-adaptative adjustment part
1751, refer to shown in Figure 13 a-13c, in the middle of the inner side of back shroud 1242, be provided with two rows raised line 1809 is installed, installing
The centre of raised line 1809 is provided with the first installation base 1812 and the second installation base 1811, offers screw thread on installation base
Hole 1802, self-adaptative adjustment part 1751 is arranged in this screwed hole 1802.
In embodiments of the present invention, described self-adaptative adjustment part 1751 is the end of thread with the connection end of back shroud, and instead
The end that connects answering storehouse assembly is the polished rod 1805 being provided with spring 1806, is provided with nut 1804 between the ends and positions,
After installation is complete, nut 1804 is close to installation base.
It is passage 1803 between two described raised lines 1809, it is possible to Reagent Tube is passed through；Back shroud also sets
It is equipped with the reinforcement gusset 1812 vertical with raised line 1809；Upper surface 1810 bit plane at raised line 1809 with boss；At plate below
The centre of peripheral framework 1807 and corner be provided with trip bolt hole 1801, in order to described turning reaction warehouse even
Connect fixing.
Above-mentioned back shroud assembly, is not only able to reaction warehouse assembly is carried out face seal, additionally it is possible to installs fixing self adaptation and adjusts
One integral piece, simple in construction.
In embodiments of the present invention, it is provided with self-adaptative adjustment part between turning reaction warehouse and reaction warehouse base 126
1751, refer to shown in Figure 12, the end spring of self-adaptative adjustment part 1751 and the end contact of reaction warehouse base；Using
During CCD camera feeding, when camera and reaction chip component touch, when adjusting camera position, movable side can be used
Formula adjusts, and now, self-adaptative adjustment part can change the position of reaction chip assembly according to the change in location of camera, it is ensured that has
Higher accuracy of detection, simultaneously, it is to avoid system is caused damage.
In embodiments of the present invention, in order to ensure that turning reaction warehouse assembly can coaxially move with reaction chip assembly,
Convenient adjustment, is additionally provided with guide post 1758 between the two, refers to, shown in Figure 11 c, arrange guide post on reaction warehouse base
Hole 1742, arranges guide post installing hole 1786 on turning warehouse, and guide post 1742 sequentially passes through holes.Described guide post
1758 include reaction warehouse locating flange 1757, in order to position with guiding rod aperture 1742；At the other end by nut 1755 and guiding
Bar installing hole 1786 end face location, between be additionally provided with pad 1756.
In embodiments of the present invention, in order to test the diverse location of reaction chip, employing can change survey
The sealing ring 1713 of examination area；Referring to shown in Figure 14 a-14c, described sealing ring 1713 is hexagon, and it includes outer rim
1716 and be arranged on the first blend stop 1714 and the second blend stop 1715 of opposite side, the first blend stop 1714 and the second blend stop 1715 will examination
Agent is divided into three parts.In an embodiment, sidepiece rib and blend stop upper end be respectively first protruding 1718 and second
Protruding 1719, two projections make reagent flow in the range of limiting, and reagent liquid inlet and outlet is arranged between two blend stops
In chamfering.When not using blend stop, reagent flows into inside whole sealing ring by the chamfering between two blend stops, now, it is possible to
Observe the reaction of whole reaction chip；When state as shown in figures 14a, reaction chip between two blend stops can only be observed
Region；When state as shown in figure 14 c, can only region on the right side of observing response chip.
In embodiments of the present invention, when described reaction warehouse assembly 12 works, above-mentioned reaction chip assembly is arranged on can
In upset reaction warehouse, install guide post 1758 and self-adaptative adjustment part 1751, and packaged back shroud；With turnable basement
After assembly 122 installation, lock turning reaction warehouse and door frame by electronic lock assembly and pressing piece, and will peace
The reaction warehouse assembly 12 installed is arranged on platform assembly 13.
Referring to shown in Figure 15 a-15b, the platform assembly of the embodiment of the present invention includes vibration damper plate 1497 and is arranged on
First damping wing plate 1492 and the second damping wing plate 1491 of vibration damper plate 1497 both sides, in the lower end of two damping wing plates respectively
It is provided with dampening assembly 1493.After installation is complete, platform assembly is indentations, and reaction warehouse assembly 12 is arranged on vibration damper plate
On 1497, two ends contact with two vibration damper plates respectively.
Damping wing plate includes gripper shoe 1497, and it has certain height, the reaction warehouse assembly described in support；Gripper shoe
The centre of 1497 integrated upper head plates 1495 is provided with the mounting groove 1492 of motion control unit 15, is provided with motion in it
The circuit board of control unit 15 and various circuit；It is reinforcing plate 1496 in the lower end of mounting groove 1492, in order to offer mounting groove
1492.The platform assembly of the embodiment of the present invention can either be symmetrical arranged the stability of guarantee system, can arrange again control
Assembly, saves space, compact conformation.
Platform assembly 13 is additionally provided with motion platform assembly 14 and CCD camera 101, refers to shown in Figure 16,
Described motion platform assembly 14 includes lead screw guide rails assembly that motor 136 is connected with motor output shaft, supports lead screw assembly
Support seat and detection components, camera support assembly.
Referring to shown in Figure 17, the output shaft of motor 136 is connected by shaft coupling 1377 with leading screw 137, motor and leading screw
Junction is provided with support seat 1361, is provided with bearing 1371 and axial positioning sleeve 1376 in the end of leading screw.On leading screw
Connecting and have nut 1374, nut is arranged in nut mounting seat 1375；The upper end of nut mounting seat 1375 is with connecting plate 1012 even
Connecing, the upper end of connecting plate 1012 connects camera mounting seat 1011；At bearing end, bearing friction top 1372 is set.
Described nut mounting seat 1375 is arranged on the slide block 1012 contacted with guide rail 132, and drives phase on slide block
Machine moves.
Referring to Figure 18 a, it is the structural representation of nut mounting seat, and its upper end is provided with connecting plate installing hole 1401,
Mid portion is provided with through hole 1402, in order to through leading screw；The surrounding of through hole 1402 for fastening screwed hole 1403, in order to nut
Install.
Referring to shown in Figure 18 b, it is the structural representation supporting seat of motor and leading screw junction, and it includes that leading screw is pacified
Sleeved 1363, positioning step 1364 is set in it；Install sleeve end connect have installing plate 1362, in order to motor end face
Connect.
Referring to shown in Figure 18 c, the detection components in the embodiment of the present invention includes that detecting plate fixes seat 1353, and it is arranged on
The sidepiece of connecting plate 1012, the end connection of seat 1353 fixed by detection plate has position to detect plate 1352；Sidepiece at base is installed
Position sensor mounting seat 1351, arranges contiguous block 1356, contiguous block upper end in the upper end of position sensor installation seat 1351
It is fixed with position sensor 135.
Referring to shown in Figure 18 d, it is the structural representation of position sensor mounting seat of the present invention, and it includes being arranged on side
The screwed hole 1415 in face 1411, is mounting groove 1412 in the centre of mounting seat, in order to installation site sensor；Sidepiece baffle plate with under
Slide recess 1413 it is provided with between bottom surface.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, and this embodiment is also not used to
Limiting the scope of the claims of the present invention, all equivalences done without departing from the present invention are implemented or change, are intended to be limited solely by the technology of the present invention
In the range of scheme.
Claims (10)
1. a DNA sequencer reacting record subsystem, it is characterised in that it includes reaction warehouse assembly, motion platform assembly, fortune
Dynamic control unit, CCD camera and platform assembly, wherein,
Described reaction warehouse assembly, arranges reaction chip assembly in it, under the effect of control system, reactant liquor enters reaction core
Sheet carries out chemical reaction, produces visible ray；
Described motion platform assembly, CCD camera is fed into suitable picture-taking position by it, so that CCD camera is to reaction chip
The visible ray information of middle generation carries out collection of taking pictures；
Described platform assembly, its support described in reaction warehouse assembly, motion platform assembly；
Described motion control unit, the operation of CCD camera and reaction chip is controlled by real time, drives motion platform assembly
Move to the picture-taking position of CCD camera, detect and control the temperature of reaction warehouse；
Described reaction warehouse assembly includes the vertical door frame being arranged on described platform assembly, in the outside of door frame
Top is provided with turning reaction warehouse assembly, and connecting in the lower end of turning reaction warehouse assembly has turnable basement, the turning end
Seat is fixed on the middle part of door frame, and turning reaction warehouse assembly can rotate around described turnable basement, in order to take out or to put into
Reaction chip；
Described turning reaction warehouse assembly includes turning warehouse, and the inner side of turning warehouse is provided with reaction chip group
Part, is provided with reaction chip in reaction chip assembly, and reagent solution passes through reaction chip generation chemical reaction.
DNA sequencer reacting record subsystem the most according to claim 1, it is characterised in that described platform group
Part includes vibration damper plate and is arranged on the first damping wing plate and the second damping wing plate of vibration damper plate both sides, at two damping wing plates
Lower end is respectively arranged with dampening assembly；
Platform assembly is indentations, and reaction warehouse assembly is arranged on vibration damper plate, and two ends contact with two vibration damper plates respectively.
DNA sequencer reacting record subsystem the most according to claim 2, it is characterised in that described damping wing plate bag
Including gripper shoe, it has certain height, the reaction warehouse assembly described in support；
The centre of the integrated upper head plate of gripper shoe is provided with the mounting groove of motion control unit, is provided with motor control in it
The circuit board of unit and various circuit；
It is reinforcing plate in the lower end of mounting groove, in order to offer mounting groove.
DNA sequencer reacting record subsystem the most according to claim 2, it is characterised in that described motion platform group
Part include the lead screw guide rails assembly that motor is connected with motor output shaft, the support seat supporting lead screw assembly and detection components,
Camera support assembly；
The output shaft of motor is connected by shaft coupling with leading screw, and motor and leading screw junction are provided with support seat, at the end of leading screw
Portion is provided with bearing and axial positioning sleeve；
Connecting on leading screw has nut, nut to be arranged in nut mounting seat；The upper end of nut mounting seat is connected with connecting plate, even
The upper end of fishplate bar connects camera mounting seat；At bearing end, bearing friction top is set；
Described nut mounting seat be arranged on on the slide block of guide rail contact, and on slide block drive CCD camera move.
DNA sequencer reacting record subsystem the most according to claim 4, it is characterised in that described detection components bag
Including detection plate and fix seat, it is arranged on the sidepiece of connecting plate, and the end connection of seat fixed by detection plate has position to detect plate；At base
Sidepiece position sensor mounting seat is installed, contiguous block is set in the upper end of position sensor installation seat, contiguous block upper end is solid
Determine position sensor；
Described sensor installation seat includes the screwed hole being arranged on side, is mounting groove in the centre of mounting seat, in order to install
Position sensor；It is provided with slide recess between sidepiece baffle plate and bottom surface.
6. according to the DNA sequencer reacting record subsystem described in claim 2 or 4, it is characterised in that described turning
The bottom of warehouse is provided with the first rotating part, and it includes the first receiving chamber, and in order to accommodate turnable basement, first accommodates chamber is arranged
Centre position in bottom；
Accommodating both sides, chamber first is symmetrically arranged first lug, and the centre of the first lug is provided with through hole；In order to institute
The turnable basement stated connects.
7. according to the DNA sequencer reacting record subsystem described in claim 2 or 4, it is characterised in that reaction chip assembly bag
Include reaction chip mounting seat, reaction warehouse base assembly and reaction warehouse base assembly and reaction chip mounting seat are locked, opened
Shading positioning component；
Mid portion in reaction chip mounting seat is provided with reaction chip, and the side portion in reaction chip mounting seat is provided with
Mounting seat props up support component, and it is in order to taking out or during chip placement, the reaction chip mounting seat described in support.
8. according to the DNA sequencer reacting record subsystem described in claim 2 or 4, it is characterised in that at described door frame
The left side of upper end is additionally provided with electronic lock assembly, and electronic lock assembly includes electronics lock and inductive switch, described inductive switch
Being arranged on electronics lock, electronics lock is arranged on the sidepiece of turning warehouse；
Sidepiece at described turning warehouse is provided with dead bolt installing hole；Dead bolt enters after stretching out and is arranged on turning warehouse side
In the dead bolt hole in portion.
DNA sequencer reacting record subsystem the most according to claim 7, it is characterised in that in reaction warehouse base
Between position be provided with electrical heating wire, it is arranged in the heating wire mounting groove of base；Temperature sensor on electrical heating wire top
Mounting groove is provided with temperature sensor and controls assembly, in order to detect the temperature of reaction tank and to adjust according to the threshold value set
Joint；Also watering be inoculated with sealing member in temperature sensor and control assembly, it is encapsulating resin.
DNA sequencer reacting record subsystem the most according to claim 7, it is characterised in that at turning reaction warehouse
And being provided with self-adaptative adjustment part between reaction warehouse base, the end spring of self-adaptative adjustment part connects with the end face of reaction warehouse base
Touch；Self-adaptative adjustment part can change the position of reaction chip assembly according to the change in location of camera；
Turning reaction warehouse assembly coaxially moves with reaction chip assembly, is additionally provided with guide post between the two.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716526.4A CN105273979B (en) | 2015-10-29 | 2015-10-29 | A kind of DNA sequencer reacting record subsystem |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716526.4A CN105273979B (en) | 2015-10-29 | 2015-10-29 | A kind of DNA sequencer reacting record subsystem |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105273979A CN105273979A (en) | 2016-01-27 |
| CN105273979B true CN105273979B (en) | 2016-11-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510716526.4A Expired - Fee Related CN105273979B (en) | 2015-10-29 | 2015-10-29 | A kind of DNA sequencer reacting record subsystem |
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| CN (1) | CN105273979B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020034143A1 (en) * | 2018-08-16 | 2020-02-20 | 深圳华大智造科技有限公司 | Handle device, positioning device, loading device and gene sequencer |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703312B (en) * | 2012-05-24 | 2014-03-19 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703311B (en) * | 2012-05-24 | 2013-08-21 | 中国科学院北京基因组研究所 | Reaction bin for adaptive regulation of DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703314B (en) * | 2012-05-24 | 2013-09-04 | 中国科学院北京基因组研究所 | Control system for DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102766574B (en) * | 2012-05-24 | 2013-12-25 | 中国科学院北京基因组研究所 | Reaction chamber for DNA sequenator |
| CN102703302B (en) * | 2012-05-24 | 2013-11-27 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
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- 2015-10-29 CN CN201510716526.4A patent/CN105273979B/en not_active Expired - Fee Related
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| CN105273979A (en) | 2016-01-27 |
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Granted publication date: 20161116 Termination date: 20191029 |