CN105296345B - A kind of DNA sequencer turning reaction warehouse device - Google Patents
A kind of DNA sequencer turning reaction warehouse device Download PDFInfo
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- CN105296345B CN105296345B CN201510711551.3A CN201510711551A CN105296345B CN 105296345 B CN105296345 B CN 105296345B CN 201510711551 A CN201510711551 A CN 201510711551A CN 105296345 B CN105296345 B CN 105296345B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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Abstract
The present invention relates to a kind of DNA sequencer turning reaction warehouse device, it includes turning warehouse, in the inner side of the centre of described turning warehouse, reaction chip assembly is installed, in reaction chip inner assembly, is provided with reaction chip, react with the reagent for sequenator；The first cavity accommodating reaction chip assembly it is provided with, in order to position described reaction chip assembly in the centre of turning warehouse；The bottom of the first cavity is provided with the first boss, first boss is in order to carry described reaction chip assembly, being provided with four guide post installing holes on the first boss, guide post installing hole passes the two ends of turning warehouse, reaction chip assembly, is connected with turning warehouse by reaction chip assembly.The DNA sequencer turning reaction warehouse device of the present invention has the robust structure of many recanalization, easy for installation between each structure, compact conformation, and stability is high.
Description
Technical field
The present invention relates to DNA sequencing field, particularly relate to a kind of DNA sequencer turning reaction warehouse device.
Background technology
In DNA sequencing technical field, general by installing reaction chip, sequencing procedure on the securing means
In, sequence testing chip occurs chemical reaction, produces visible ray, by CCD (Charge Couple Device)
Optical signal produced by cameras capture sequencing reaction, i.e. available required order-checking information.
In sequenator, the reacting record subsystem information to sequencing reaction, it is desirable to reaction warehouse has
Having the adjustable of enough precision and installation, reaction warehouse is as the main carriers of test reaction, it should tool
There is the feature of convenient adjustment, further, it is possible to continue, carry out reagent reacting reliably.
In view of drawbacks described above, creator of the present invention obtains this creation finally through research for a long time and practice.
Summary of the invention
It is an object of the invention to provide a kind of DNA sequencer turning reaction warehouse device, above-mentioned in order to overcome
Technological deficiency.
For achieving the above object, the present invention provides a kind of DNA sequencer turning reaction warehouse device, and it includes
Turning warehouse, is provided with reaction chip assembly in the inner side of the centre of described turning warehouse, reacts core
It is provided with reaction chip in sheet inner assembly, reacts with the reagent for sequenator；
The first cavity accommodating reaction chip assembly it is provided with in the centre of turning warehouse, described in order to position
Reaction chip assembly；Be provided with the first boss in the bottom of the first cavity, the first boss is described in order to carry
Reaction chip assembly, the first boss is provided with four guide post installing holes, guide post installing hole passes
The two ends of turning warehouse, reaction chip assembly, be connected reaction chip assembly with turning warehouse；
The bottom of turning warehouse is provided with the first rotating part, and it includes the first receiving chamber, accommodates chamber first
Both sides are symmetrically arranged first lug, and the centre of the first lug is provided with through hole；
Reagent Tube the first fixation kit it is provided with, in order to fixing in the through hole of the centre of the first described lug
Reagent Tube.
Further, described Reagent Tube the first fixation kit include being arranged on the pipe joint in the lug of left side,
First packing ring, the first rotary shaft, and be arranged on the Reagent Tube joint in the lug of right side, the second packing ring, the
Two rotary shafts；
The inner side of through hole and the inner side of packing ring that arrange in the middle of the first described lug are provided with the first rotation
Rotating shaft and the second rotary shaft, the structure of two rotary shafts is identical, is the quill shaft with positioning convex ring；?
One end of rotary shaft arranges positioning convex ring, is provided with connection screwed hole, in order to described turning storehouse
The lug connection portion of body connects fixing, accordingly, is provided with location in the end of described through hole described fixed
The positioning recess of position bulge loop.
Further, it is recess in the centre of the first described rotary shaft and the both ends of the second rotary shaft, interior
Side recess is in order to install location with described pipe joint, Reagent Tube joint；Connect at pipe joint, Reagent Tube
Being respectively mounted live wire and Reagent Tube in Tou, it respectively leads in reaction chip assembly.
Further, the upper end in the outside of described turning warehouse is provided with pressing piece, described pressing
Part is that one end is provided with opening, one end is provided with the structure of hook, the upper end-stopping plate of described hook and door frame
Connecting, when pressing piece is by downward active force, hook departs from door frame baffle plate, turning reaction warehouse
Can rotate relative to door frame.
Further, described pressing piece is arranged in a positioning block, and the lower end of locating piece is provided with spring,
Spring is arranged in spring base, and spring base is threadably mounted at the intermediate throughholes upper surface of turning reaction warehouse
In spring base hole；
Described locating piece is arranged in the locating piece installation cavity of turning reaction warehouse upper end, sets in cavity
It is equipped with positioning step；Locating piece includes through hole and lower through-hole, and one of both is by described pressing piece；?
The side of locating piece arranges locating and machining hole and installing hole；It is provided with in the lower end of the through hole installing pressing piece
Spring eye, spring is contacted with the bottom of pressing piece by this hole.
Further, the mid portion at described turning warehouse is provided with back shroud, in order to encapsulate reagent
Pipe, and adjust reaction warehouse assembly and the position of turning warehouse by self-adaptative adjustment part thereon；
Described self-adaptative adjustment part includes spring, spring end and reaction warehouse component touch, reaction warehouse assembly
Compression spring, adjusts self-position；
The self-adaptative adjustment part stated is arranged on the centre position, inner side of back shroud, in the inner side of described back shroud
Centre is provided with two rows and installs raised line, is provided with the first installation base and second in the centre installing raised line and installs
Boss, offers screwed hole on installation base, and self-adaptative adjustment part is arranged in this screwed hole.
Further, the upper end in the outside of described upset warehouse is provided with pressing piece, described pressing piece
One end is provided with opening, one end is provided with the structure of hook, and the upper end-stopping plate of described hook and door frame is even
Connecing, when pressing piece is by downward active force, hook departs from door frame baffle plate, turning reaction warehouse physical ability
Enough rotate relative to door frame；
Described pressing piece is arranged in a positioning block, and the lower end of locating piece is provided with spring, and spring is arranged on
In spring base.
Further, described reaction chip assembly include reaction chip mounting seat, reaction warehouse base assembly with
And shading positioning component reaction warehouse base assembly and reaction chip mounting seat locked, opens；Described is anti-
Answer chip mounting seat can rotate against with reaction warehouse base assembly, and be fixed on an angle.
Further, in the medial surface of described reaction warehouse base, it is provided with reaction cell body, at reaction cell body
Mid portion arranges reaction tank, and the surrounding at reaction tank is provided with sealing ring；
The mid portion of the lateral surface of described reaction warehouse base is provided with temperature control assembly, at reaction warehouse base
Mid portion is provided with temperature sensor mounting groove, and the bottom of mounting groove is provided with heating wire mounting groove, point
Fang Zhi heating wire and temperature sensor.
Further, the surrounding at described temperature sensor mounting groove is provided with four guide post installing holes,
Upper and lower sides is provided with self adaptation spring mounting hole；On the cover board it is also equipped with guiding rod aperture.
Compared with prior art the invention have the benefit that the turning reaction warehouse of DNA sequencer of the present invention
Device has the robust structure of many recanalization, easy for installation between each structure, compact conformation, and stability is high.
Reaction warehouse assembly can install, fixing and reaction chip described in adjusting, it is ensured that reaction chip is at stable ring
Border is reacted, and, reaction chip can conveniently take out and lock.
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of the DNA sequencer reacting record subsystem of the present invention；
Fig. 2 is the first perspective view of the reaction warehouse assembly of the present invention；
Fig. 3 is the second perspective view of the reaction warehouse assembly of the present invention；
Fig. 4 be the reaction warehouse assembly of the present invention face structural representation；
Fig. 5 a is the perspective view of the turning reaction warehouse assembly of the present invention；
Fig. 5 b is the first perspective view of the turning warehouse of the present invention；
Fig. 5 c is the second perspective view of the turning warehouse of the present invention；
Fig. 5 d is the first perspective view of the locating piece of the present invention；
Fig. 5 e is the second perspective view of the locating piece of the present invention；
Fig. 6 a is the structural representation of the electronic lock of the present invention；
Fig. 6 b is the structural representation of the electronics lock of the present invention；
Fig. 6 c is the sectional structure schematic diagram of the electronic lock of the present invention；
Fig. 7 is the turning reaction warehouse assembly sectional structure schematic diagram along the first lug of the present invention；
Fig. 8 is the 3rd perspective view of the turning warehouse of the present invention；
Fig. 9 a is the structural representation of the reaction chip assembly of the present invention；
Fig. 9 b is the structural representation of the reaction chip mounting seat of the present invention；
Fig. 9 c is the structural representation of the medial surface of the reaction warehouse base of the present invention；
Fig. 9 d is the structural representation of the lateral surface of the reaction warehouse base of the present invention；
Fig. 9 e is the structural representation of the temperature control assembly cover plate of the present invention；
Figure 10 a is the structural representation of the shading positioning component of the present invention；
Figure 10 b is the first structural representation of the slidably positioning and locking block of the present invention；
Figure 10 c is the second structural representation of the slidably positioning and locking block of the present invention；
Figure 10 d is the structural representation of the turnable basement assembly of the present invention；
Figure 10 e is the structural representation of the spring assembly of the present invention；
Figure 11 a is the sectional structure schematic diagram along liquid inlet and outlet of the reaction chip assembly of the present invention；
Figure 11 b is the turning reaction warehouse assembly sectional structure schematic diagram along self-adaptative adjustment part of the present invention；
Figure 11 c is the turning reaction warehouse assembly sectional structure schematic diagram along guide post of the present invention；
Figure 12 is that the turning reaction warehouse assembly of present invention A-A along Fig. 4 is to sectional structure schematic diagram；
Figure 13 a is the sectional structure schematic diagram of the back shroud of the present invention；
Figure 13 b is the perspective view of the back shroud assembly of the present invention；
Figure 13 c is the perspective view of the back shroud of the present invention；
Figure 14 a is the structural representation of the sealing ring of the present invention；
Figure 14 b is the sealing ring sectional structure schematic diagram of the present invention；
Figure 14 c is the use view that the present invention observes the sealing ring of right side reaction chip.
Detailed description of the invention
Below in conjunction with accompanying drawing, to the present invention, above-mentioned and other technical characteristic and advantage are described in more detail.
Referring to shown in Fig. 1, it is that the overall structure of DNA sequencer reacting record subsystem of the present invention is shown
Be intended to, the reacting record subsystem in the embodiment of the present invention include reaction warehouse assembly 12, motion platform assembly 14,
Motion control unit 15, CCD camera 101 and platform assembly 13, wherein,
Described reaction warehouse assembly 12, arranges reaction chip assembly in it, under the effect of control system, instead
Answer liquid to enter in reaction chip and carry out chemical reaction, produce visible ray；Described motion platform assembly 14, its
CCD camera is fed into suitable picture-taking position, the visible ray information produced in reaction chip is taken pictures
Gather；Described platform assembly 13, its support described in reaction warehouse assembly 12, motion platform assembly 14；
Described motion control unit, the operation of CCD camera and reaction chip is controlled by real time.
In embodiments of the present invention, described reaction warehouse assembly 12 can install, fixing and anti-described in adjusting
Answer chip, it is ensured that reaction chip reacts in stable environment, and, reaction chip can conveniently take
Go out and lock.
Referring to shown in Fig. 2-4, described reaction warehouse assembly 12 includes being arranged on described platform assembly
The door frame 121 being vertically arranged on 13, is provided with turning reaction warehouse group on the top in the outside of door frame 121
Part 124, connects in the lower end of turning reaction warehouse assembly 124 and has turnable basement 122, turnable basement 122
Being fixed on the middle part of door frame 121, turning reaction warehouse assembly 124 can revolve around described turnable basement 122
Turn, in order to take out or to put into reaction chip.
It is additionally provided with electronic lock assembly 125, only in electronic lock group in the left side of the upper end of described door frame 121
When part 125 is opened, turning reaction warehouse assembly 124 can be rotated, reaction chip is operated.
In embodiments of the present invention, it is provided with door frame 121, supports reaction chip and dependency structure, substantially increase and be
The stability of system.
Turning reaction warehouse assembly 124 includes turning warehouse, at the mid portion of described turning warehouse
Back shroud 1242 is installed, in order to encapsulate Reagent Tube, and is adjusted instead by self-adaptative adjustment part 1751 thereon
Answer storehouse assembly and the position of turning warehouse.
The bottom of turning warehouse is provided with the first rotating part, and it includes the first receiving chamber 1243, in order to accommodate
Turnable basement 122, first accommodates chamber 1243 is arranged on the centre position of bottom；Both sides, chamber are accommodated first
For symmetrically arranged first lug 1244, the centre of the first lug 1244 is provided with through hole 1246；In order to
It is connected with described turnable basement 122.The most turning warehouse of turnable basement accommodates chamber 1243 first
Interior rotation, when turning warehouse turns to horizontal level, operates reaction chip assembly；Have higher steady
Qualitative, ensure that the transmission of reagent and data is unimpeded simultaneously.
Refer to shown in Fig. 5 a-5c, in the inner side of the centre of described turning warehouse, reaction chip is installed
Assembly, accordingly, is provided with the first cavity 1785 accommodating reaction chip assembly in the centre of turning warehouse；
Be provided with the first boss 1784 in the bottom of the first cavity 1785, the first boss 1784 is described in order to carry
Reaction chip assembly, is provided with four guide post installing holes 1786 on the first boss 1784, and guide post is installed
Hole 1786 is through the two ends of turning warehouse, reaction chip assembly, by reaction chip assembly and turning warehouse
Connect；Be provided with the second boss 1783 in the upper end of the first described boss 1784, the second boss 1784 is used
With the support member described in location；The second described boss 1784 upper end is provided with locating surface 1782, in location
Locking screw nail 1787 it is provided with on face 1782.Reaction chip assembly described in above-mentioned first cavity body structure location,
Further, reaction chip assembly is when normally working, it is impossible to enough rotations, is merely able to move along guide post.
The upper surface in the first receiving chamber 1243 of described turning warehouse is provided with Reagent Tube installing hole
1788 and electric wire installing hole, Reagent Tube installing hole 1788 is provided with pipe clamp 03, through having in pipe clamp 03
Reagent Tube 02 and electric wire 01.
The dead bolt of the dead bolt 1252 that the sidepiece at described turning warehouse is additionally provided with in electronic lock assembly 125
Installing hole；Under the effect of locking member, turning warehouse is fixed together with door frame 121.
Incorporated by reference to shown in Fig. 6 a and 6b, the electronic lock assembly 125 of the present invention includes that electronics lock and sensing are opened
Closing 1251, described inductive switch is arranged on electronics lock, and electronics lock is arranged on the sidepiece of turning warehouse,
In embodiments of the present invention, electronics lock includes sidepiece installing plate 1843, offers on sidepiece installing plate 1483
Installing hole 1844；Electronics lock is fixed on turning warehouse by installing hole 1844 and installation screw.?
Described sidepiece installing plate 1483 side is provided with the first framework 1841 and the second framework 1842 of symmetry,
The space installing inductive switch 1251 is left in the centre of the first framework and the second framework.
In embodiments of the present invention, described inductive switch 1251 includes the framework 1255 being arranged on outside, frame
Being provided with connection screwed hole 1256 on the one side of body 1255, it passes through attachment screw with described electronics lock
1254 fix；Described attachment screw 1254 by the lug 1258 on side and the first framework 1841, the
Two frameworks 1842, in order to increase the bonding strength of electronics lock.Described inductive switch 1251 is also connected with
There is control circuit 1252, in order to inductive switch 1251 to be controlled.
Referring to shown in Fig. 6 c, described electronic lock assembly 125 is arranged on turning warehouse and door frame 121
Sidepiece, dead bolt 1253 enters in the dead bolt hole 1247 being arranged on turning warehouse sidepiece after stretching out, realizes locking；
When dead bolt 1253 is under the effect of electricity irritation, when retracting in dead bolt hole 1247, can rotate described can
Upset warehouse.
The upper end in the outside of described turning warehouse is provided with pressing piece 1241, only passes through pressing piece
1241 pressings arrange and described turning warehouse can be made to rotate.Refer to shown in Figure 12, real in the present invention
Executing in example, described pressing piece 1241 is provided with opening 1730 for one end, one end is provided with the structure of hook,
Described hook is connected with the upper end-stopping plate of door frame, when pressing piece 1241 is by downward active force, and hook
Departing from door frame baffle plate, turning reaction warehouse body can rotate relative to door frame.
In embodiments of the present invention, described pressing piece 1241 is arranged in a positioning block 1762, locating piece
The lower end of 1762 is provided with spring 1761, and spring is arranged in spring base 1763, and spring base 1763 screw thread is pacified
It is contained in the spring base hole 1781 of intermediate throughholes 1785 upper surface of turning reaction warehouse.
Incorporated by reference to shown in Fig. 5 b-5d, locating piece 1762 is arranged on the locating piece peace of turning reaction warehouse upper end
It behave affectedly in 1791, in cavity, be provided with positioning step 1790.Locating piece 1762 in the embodiment of the present invention
Including upper through hole 1794 and lower through-hole 1797, one of both is by described pressing piece 1241；At locating piece
Side locating and machining hole 1796 and installing hole 1795 are set.Under the through hole installing pressing piece 1241
End is provided with spring eye, and spring is contacted by the bottom in this hole with pressing piece 1241.
The embodiment of the present invention arranges electronic lock assembly and pressing piece, to turning reaction warehouse assembly and door frame
Carry out twice locking, be respectively adopted automatically controlled and frame for movement mode, it is ensured that reaction chip is in security context
Run, it is to avoid maloperation.
Referring to shown in Fig. 2 and 7, the turnable basement 122 in the embodiment of the present invention is for having two second
The connection base of lug 1221, the second lug 1221 is symmetricly set on a side 1223 of base, its
Centre is provided with through hole 1224, the middle setting with the first lug 1244 on described turning warehouse
Through hole 1246 is co-axially mounted, and, the second lug 1221 is arranged on the inner side of the first lug 1244.Can turn over
Turning base 122 is the intermediate connector arranged, and turning warehouse realizes turning over by this turning connection seat 122
Turn and position, easy to operate.
Incorporated by reference to shown in Fig. 2, in embodiments of the present invention, door frame 121 has sidepiece baffle plate 1211 for one side
Structure, it is one-body molded, and the both sides of described turnable basement 122 are arranged in sidepiece baffle plate 1211 also
Location is contacted with sidepiece baffle plate 1211.
The first described lug 1224 after being co-axially mounted with the second lug 1221, end and turnable basement
The upper-end contact of 122, and, the sidepiece of turning warehouse and sidepiece baffle plate 1211 medial surface of door frame 121
Contact, it is ensured that turning warehouse and turnable basement 122 are located just in sidepiece baffle plate 1211.
Door frame 121 is additionally provided with the receiving hole passed through for reaction chip assembly.The use of door frame, not only
Enable to the Stability Analysis of Structures of reaction chip assembly, and, also make turning warehouse tie with turnable basement
Structure is compact, it is simple to operation；CCD camera is more accurate with the location of reaction chip.
The first installing hole 1210 it is provided with in the bottom of door frame 121, solid in order to be connected with motion platform assembly 14
Fixed；The second installing hole being connected with turnable basement sidepiece it is additionally provided with in the middle part of door frame.
Incorporated by reference to shown in Fig. 7, in embodiments of the present invention, in order to save structure space and ensure that reagent passes
Defeated stablizes, the through hole 1246 arranged in the middle of the first described lug 1244 and the second lug 1221
Being provided with Reagent Tube the first fixation kit in through hole, it includes the pipe joint being arranged in the lug of left side
1265, first packing ring the 1264, first rotary shaft 1261, and it is arranged on the Reagent Tube joint in the lug of right side
1266, second packing ring the 1267, second rotary shaft 1269, wherein, the first described packing ring 1264 and second
Packing ring 1267 is arranged between turning warehouse and turnable basement, and in the present embodiment, described first is convex
The diameter of the through hole 1246 arranged in the middle of ear 1244 is less than the diameter of the through hole of the second lug 1221,
Both medial ends form the location rib to packing ring location, after installing two packing rings, inside diameter of gasket
Just identical with the diameter of the through hole of the second lug 1221.In embodiments of the present invention arrange location rib with
And packing ring, it is ensured that gapless between turning warehouse and turnable basement, both good stabilities after combining,
Will not rock because there is gap, and, packing ring adds both contacts area so that between the two
Stress reduce, extension device service life.
The inner side of through hole 1246 arranged in the middle of the first lug 1244 and the inner side of packing ring are provided with the
One rotary shaft 1261 and the second rotary shaft 1269, the structure of two rotary shafts is identical, is with positioning convex ring
Quill shaft；Positioning convex ring is set in one end of rotary shaft, is provided with connection screwed hole, in order to institute
The lug connection portion 1263 of the turning warehouse stated connects fixing, accordingly, at described through hole 1246
End be provided with the positioning recess positioning described positioning convex ring.In the centre at the both ends of rotary shaft 1269 it is
Recess, inner side recess is in order to install location with described pipe joint 1265, Reagent Tube joint 1266.At pipe
Being respectively mounted live wire and Reagent Tube 02 in wire terminal 1265, Reagent Tube joint 1266, it respectively leads to
In reaction chip assembly.Owing to the embodiment of the present invention arranging the assembly that can mutually overturn, when upset, for
Avoid pipe fitting is impacted, Reagent Tube is installed in through-holes, substantially increase the security performance of Reagent Tube.
Referring to shown in Fig. 8, in embodiments of the present invention, described reaction chip assembly is arranged on described
The inner side of turning warehouse.The observation portion contacted with CCD camera it is provided with in the inner side of reaction chip assembly
1724, it is provided with transparent baffle plate, in order to course of reaction is taken pictures.
Referring to shown in Fig. 9 a, the reaction chip assembly of the present invention includes reaction chip mounting seat 127, reaction
Storehouse base assembly 126 and reaction warehouse base assembly 126 and reaction chip mounting seat 127 are locked, opened
Shading positioning component 128；In order to easily reaction chip is taken out and Guan Bi, reaction chip mounting seat
127 can rotate against with reaction warehouse base assembly 126.
Referring to shown in Fig. 9 b, it is the structural representation of reaction chip mounting seat of the present invention, at reaction core
The mid portion of sheet mounting seat 127 is provided with reaction chip, is provided with cover plate 1277 on reaction chip；?
The both sides of reaction chip are provided with chute pressing plate 1279, in order to be fixed reaction chip.At reaction chip
One side portion of mounting seat 127 is provided with mounting seat and props up support component, and it is in order to taking out or during chip placement,
Reaction chip mounting seat described in support；It includes torsional-spring supported portion 1273, is arranged in mounting seat, in order to
Torsion spring described in support, described torsional-spring supported portion 1273 is one with the recess of protruding end 1274, in rotation
When turning described reaction chip mounting seat 127, protruding end 1274 contacts with 1261 at first, at the present embodiment
In, described protruding end 1274 is arranged on the middle part of recess and the edge of chip installation position；Use peace
Reaction chip mounting seat is supported by the structure of dress seat itself, better reliability, it is ensured that chip can safety
Take out and place.
Described mounting seat is propped up support component and is also included that torsion spring 1272 is connected with torsion spring 1272 and is arranged on torsion spring two
The tension axle 1271 of end, described tension axle 1271 is arranged in lobe 1275, is starting reaction chip
During mounting seat, described torsion spring 1272 supports mounting seat, and finally relies on the location of protruding end 1274, real
Now support.
Be additionally provided with the first rotary shaft hole 1276 in the end of described lobe 1275, in order to reaction warehouse at the bottom of
Seat 1261 connects.Sidepiece in reaction chip mounting seat is additionally provided with locating slot 1278.
Referring to shown in Fig. 9 c, it is the structural representation of medial surface of reaction warehouse base of the present invention, instead
Answer and the medial surface of storehouse base is provided with reaction cell body 1266, reaction is set at reaction cell body 1266 mid portion
Pond 1267, the surrounding 1706 at reaction tank 1267 is provided with sealing ring, prevents reaction reagent from leaking.Instead
The one end answering storehouse base 1261 is provided with raised line 1705, in order to install after cover plate location.Set at the other end
Being equipped with the torsional-spring supported structure corresponding with reaction chip mounting seat, it includes symmetrically arranged two protruding ends
1268, it is provided with the second rotary shaft hole 1269 thereon, it is coaxial with the first rotary shaft hole 1276, rotary shaft
Sequentially pass through holes；The region 1260 movable for torsion spring and region 1707, location it is additionally provided with in this end,
It is provided with in this region in order to the screwed hole 1712 by installing reaction warehouse cover plate.
Referring to shown in Fig. 9 d, it is the structural representation of lateral surface of reaction warehouse base of the present invention, instead
The mid portion answering the lateral surface of storehouse base is provided with temperature control assembly, and the mid portion at reaction warehouse base is arranged
There is temperature sensor mounting groove 1262, the bottom of mounting groove 1262 be provided with heating wire mounting groove 1704,
Place heating wire and temperature sensor respectively.Being additionally provided with a positioning boss 1703 in mounting groove, it is used
Position during to install with temperature control assembly cover plate 1263.As shown in figure 9e, temperature control assembly cover plate 1263 sets
It is equipped with the positioning recess 1702 corresponding with positioning boss 1703.
Surrounding at temperature sensor mounting groove 1262 is provided with four guide post installing holes 1742, at upper and lower sides
It is provided with self adaptation spring mounting hole 1741；Cover plate 1263 is also equipped with guide rod aperture 1701.
Reaction warehouse base is additionally provided with shading positioning component mounting groove 1265, in order to install described shading
Positioning component 128, is within it provided with installation base 1744.
Referring to shown in Figure 10 a, it is the structural representation of shading positioning component of the present invention, and it includes can
Slip positioning and locking block 1281 and turnable basement assembly 1283, wherein, described slidably positioning and locking block
Locking jaw 1282, after installation is complete, described slidably positioning and locking block 1281 it is provided with on 1281
Upper surface contacts with the medial surface of reaction chip mounting seat 127, and locking jaw 1282 is hooked on reaction chip and installs
The outboard end of seat 127；Described turnable basement assembly 1283 is arranged on the medial end of reaction warehouse base,
It is provided with screwed hole 1830.After installation is complete, at the bottom of described shading positioning component 128 and reaction warehouse
Seat is fixed together, and when opening reaction chip mounting seat, outwards presses slidably positioning and locking block 1281,
Under the action of the spring, slidably positioning and locking block 1281 is outward-dipping, and locking jaw more than 1282 reaction warehouse is pacified
Dress seat departs from, and under the effect of the torsion spring of reaction warehouse mounting seat end, reaction warehouse mounting seat overturns, can be to instead
Chip is answered to be replaced.
Incorporated by reference to shown in Figure 10 b, described slidably positioning and locking block 1281 includes the pressing being arranged on top
The elastic locking assembly of plate 1823 and bottom homonymy, company convex under the mid portion of pressing plate 1823 is
Junction 1821, it coordinates with described turnable basement assembly 1283.Incorporated by reference to shown in Figure 10 c, described
Elastic locking assembly includes symmetrically arranged first spring base the 1822, second spring base 1828, is arranged on two
The first recess 1827 between spring base；Spring assembly, spring assembly it is respectively arranged with in two spring bases
Including the upper spring card slot 1281 shown in Figure 10 e, lower spring draw-in groove 1283, upper and lower two spring card slots surround
Spring eye 1771, spring eye is shoulder hole, is provided with spring 1820 in it；Wherein, at upper spring card slot 1281
On be provided with external part, it arranges hook claw, in order to be connected with the end of described pressing plate 1823.
In embodiments of the present invention, spring base and the first recess isolated by continuous print vertical baffle 1826 and
Becoming, both sides arrange vertical rib 1829；Elastic locking assembly is arranged on integrated with pressing plate first flat
On plate 1825, it is vertically arranged with pressing plate 1823.
Refer to shown in Figure 10 d, the slidably positioning and locking block 1281 in the embodiment of the present invention and the turning end
Holder assembly 1283 can symmetrical coordinate, and is provided with the first projection 1832 in the centre of turnable basement, and it is with the
One recess 1827 snaps together；It is provided with in the first projection 1832 both sides and can accommodate vertical baffle 1826
Space；Accordingly, the second recess of the spring base described in receiving it is provided with in the first projection 1832 lateral symmetry
1833, the second recess is surrounded by continuous print vertical edge 1834；Said structure is arranged on integrated second
On flat board 1831；In use, spring assembly is pressed against in spring base, by slip positioning and locking block 1281
Snap together with turnable basement assembly 1283.The embodiment of the present invention, arranges shading positioning component,
It is not only able to stable locking reaction chip, and when changing reaction chip, simple to operate, only need pressing
Slip positioning and locking block 1281 is opened, and without dismantling reaction chip mounting seat, saves the time.
Refer to, shown in Figure 11 a and 11b, the middle part of reaction warehouse base be provided with electrical heating wire 1754,
It is arranged in the heating wire mounting groove 1704 of base；Temperature sensor mounting groove on electrical heating wire top
It is provided with temperature sensor 1720 in 1262 and controls assembly, in order to detect the temperature of reaction tank and according to setting
Threshold value be adjusted；Also water be inoculated with sealing member 1263 in temperature sensor 1720 and control assembly 1753,
It is encapsulating resin.
It is respectively arranged with Reagent Tube at the two ends of reaction tank and screwed hole 1721 is installed, in order to be connected with Reagent Tube；
The end installing screwed hole 1721 at Reagent Tube is provided with inlet 1723 and liquid outlet 1713, into and out of
Liquid mouth communicates with the two ends of reaction tank, in order to cycle detection reagent.
Avris in the interior grooves of reaction tank is provided with sealing ring 1713.Centre in reaction chip mounting seat
Being provided with reaction chip 04, reagent flows through reaction chip and reacts.
In embodiments of the present invention, the Reagent Tube 02 Reagent Tube installing hole 1788 by turning warehouse, arrange
Be provided with in the centre of turning warehouse accommodate reagent pipeline the first cavity 1785, final coupled reaction cell body,
Reagent cartridge is through the sealing of various cavitys, and stability is high, is hardly damaged.
Rear side at reaction warehouse is fixed with back shroud 1242, and the centre of back shroud 1242 is provided with self adaptation and adjusts
One integral piece 1751, refers to shown in Figure 13 a-13c, is provided with two rows and installs in the middle of the inner side of back shroud 1242
Raised line 1809, is provided with the first installation base 1812 and the second installation base in the centre installing raised line 1809
1811, installation base offers screwed hole 1802, self-adaptative adjustment part 1751 is arranged on this screwed hole
In 1802.
In embodiments of the present invention, described self-adaptative adjustment part 1751 is the end of thread with the connection end of back shroud,
It is the polished rod 1805 being provided with spring 1806 with the end that connects of reaction warehouse assembly, is provided with nut between the ends
1804 position, and after installation is complete, nut 1804 is close to installation base.
It is passage 1803 between two described raised lines 1809, it is possible to Reagent Tube is passed through；At back shroud
On be additionally provided with the reinforcement gusset 1812 vertical with raised line 1809；Upper surface 1810 at raised line 1809 with boss
Bit plane；Below, centre and the corner of the peripheral framework 1807 of plate are provided with trip bolt hole 1801, use
Fixing to be connected with described turning reaction warehouse.
Above-mentioned back shroud assembly, is not only able to reaction warehouse assembly is carried out face seal, additionally it is possible to installs and fixes certainly
Adapt to adjustment part, simple in construction.
In embodiments of the present invention, it is provided with self adaptation between turning reaction warehouse and reaction warehouse base 126
Adjustment part 1751, refers to shown in Figure 12, the end spring of self-adaptative adjustment part 1751 and reaction warehouse base
End contact；When using CCD camera feeding, when camera and reaction chip component touch, adjusting phase
When seat in the plane is put, can adjust in the way of employing is movable, now, self-adaptative adjustment part can be according to camera
Change in location change reaction chip assembly position, it is ensured that there is higher accuracy of detection, simultaneously, it is to avoid
System is caused damage.
In embodiments of the present invention, in order to ensure that turning reaction warehouse assembly can be coaxial with reaction chip assembly
Mobile, convenient adjustment, it is additionally provided with guide post 1758 between the two, refers to shown in Figure 11 c, in reaction
Guiding rod aperture 1742 is set on the base of storehouse, turning warehouse arranges guide post installing hole 1786, guide post
1742 sequentially pass through holes.Described guide post 1758 includes reaction warehouse locating flange 1757, in order to lead
Position to rod aperture 1742；Positioned by the end face of nut 1755 with guide post installing hole 1786 at the other end,
Pad 1756 it is additionally provided with between.
In embodiments of the present invention, in order to test the diverse location of reaction chip, employing can
Change the sealing ring 1713 of test area；Referring to shown in Figure 14 a-14c, described sealing ring 1713 is six
Limit shape, it includes outer rim 1716 and is arranged on the first blend stop 1714 and the second blend stop 1715 of opposite side,
Reagent is divided into three parts by the first blend stop 1714 and the second blend stop 1715.In an embodiment, sidepiece gear
Limit and blend stop upper end be respectively the first protruding 1718 and second projection 1719, two projections make reagent
Flowing in the range of limiting, reagent liquid inlet and outlet is arranged in the chamfering between two blend stops.Do not make
When using blend stop, reagent flows into inside whole sealing ring by the chamfering between two blend stops, now, it is possible to see
Examine the reaction of whole reaction chip；When state as shown in figures 14a, can only observe between two blend stops
The region of reaction chip；When state as shown in figure 14 c, can only region on the right side of observing response chip.
The embodiment of the present invention uses foldable sealing ring, substantially increases the motility of reaction test, can root
According to needs, reaction chip is sampled.
In embodiments of the present invention, when described reaction warehouse assembly 12 works, above-mentioned reaction chip assembly peace
Be contained in turning reaction warehouse, install guide post 1758 and self-adaptative adjustment part 1751, and packaged after
Cover plate；After turnable basement assembly 122 installation, being locked by electronic lock assembly and pressing piece can
Upset reaction warehouse and door frame, and the reaction warehouse assembly 12 of installation is arranged on platform assembly
On 13.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, and this embodiment is also
Being not used to limit the scope of the claims of the present invention, all equivalences done without departing from the present invention are implemented or change, all should
It is contained in the range of technical solution of the present invention.
Claims (9)
1. a DNA sequencer turning reaction warehouse device, it is characterized in that, it includes turning warehouse, is provided with reaction chip assembly in the inner side of the centre of described turning warehouse, it is provided with reaction chip in reaction chip inner assembly, reacts with the reagent for sequenator；
The first cavity accommodating reaction chip assembly it is provided with, in order to position described reaction chip assembly in the centre of turning warehouse；The bottom of the first cavity is provided with the first boss, first boss is in order to carry described reaction chip assembly, being provided with four guide post installing holes on the first boss, guide post installing hole passes the two ends of turning warehouse, reaction chip assembly, is connected with turning warehouse by reaction chip assembly；
The bottom of turning warehouse is provided with the first rotating part, and it includes the first receiving chamber, and accommodating both sides, chamber first is symmetrically arranged first lug, and the centre of the first lug is provided with through hole；
Reagent Tube the first fixation kit it is provided with, in order to fixating reagent pipe in the through hole of the centre of the first described lug；
The inner side of through hole and the inner side of packing ring that arrange in the middle of the first described lug are provided with the first rotary shaft and the second rotary shaft, and the structure of two rotary shafts is identical, is the quill shaft with positioning convex ring；Positioning convex ring is set in one end of rotary shaft, is provided with connection screwed hole, fixing in order to be connected with the lug connection portion of described turning warehouse, accordingly, it is provided with the positioning recess positioning described positioning convex ring in the end of described through hole；
The upper end in the outside of described turning warehouse is provided with pressing piece, described pressing piece is that one end is provided with opening, one end is provided with the structure of hook, described hook is connected with the upper end-stopping plate of door frame, when pressing piece is by downward active force, hook departs from door frame baffle plate, and turning reaction warehouse body can rotate relative to door frame.
DNA sequencer the most according to claim 1 turning reaction warehouse device, it is characterized in that, described Reagent Tube the first fixation kit includes being arranged on the pipe joint in the lug of left side, the first packing ring, the first rotary shaft, and is arranged on the Reagent Tube joint in the lug of right side, the second packing ring, the second rotary shaft.
DNA sequencer the most according to claim 2 turning reaction warehouse device, it is characterised in that be recess in the centre of the first described rotary shaft and the both ends of the second rotary shaft, inner side recess is in order to install location with described pipe joint, Reagent Tube joint；Being respectively mounted live wire and Reagent Tube in pipe joint, Reagent Tube joint, it respectively leads in reaction chip assembly.
DNA sequencer the most according to claim 3 turning reaction warehouse device, it is characterized in that, described pressing piece is arranged in a positioning block, the lower end of locating piece is provided with spring, spring is arranged in spring base, in the spring base hole of the intermediate throughholes upper surface that spring base is threadably mounted at turning reaction warehouse；
Described locating piece is arranged in the locating piece installation cavity of turning reaction warehouse upper end, is provided with positioning step in cavity；Locating piece includes through hole and lower through-hole, and one of both is by described pressing piece；In the side of locating piece, locating and machining hole and installing hole are set；The lower end of the through hole installing pressing piece is provided with spring eye, and spring is contacted with the bottom of pressing piece by this hole.
DNA sequencer the most according to claim 1 turning reaction warehouse device, it is characterized in that, mid portion at described turning warehouse is provided with back shroud, in order to encapsulate Reagent Tube, and adjusts reaction warehouse assembly and the position of turning warehouse by self-adaptative adjustment part thereon；
Described self-adaptative adjustment part includes spring, spring end and reaction warehouse component touch, reaction warehouse assembly compression spring, adjusts self-position；
The self-adaptative adjustment part stated is arranged on the centre position, inner side of back shroud, in the middle of the inner side of described back shroud, it is provided with two rows raised line is installed, the centre installing raised line is provided with the first installation base and the second installation base, offering screwed hole on installation base, self-adaptative adjustment part is arranged in this screwed hole.
DNA sequencer the most according to claim 1 turning reaction warehouse device, it is characterized in that, the upper end in the outside of described upset warehouse is provided with pressing piece, described pressing piece is that one end is provided with opening, one end is provided with the structure of hook, described hook is connected with the upper end-stopping plate of door frame, when pressing piece is by downward active force, hook departs from door frame baffle plate, and turning reaction warehouse body can rotate relative to door frame；
Described pressing piece is arranged in a positioning block, and the lower end of locating piece is provided with spring, and spring is arranged in spring base.
DNA sequencer the most according to claim 1 turning reaction warehouse device, it is characterized in that, described reaction chip assembly includes reaction chip mounting seat, reaction warehouse base assembly and the shading positioning component that reaction warehouse base assembly and reaction chip mounting seat are locked, opened；Described reaction chip mounting seat can rotate against with reaction warehouse base assembly, and is fixed on an angle.
DNA sequencer the most according to claim 7 turning reaction warehouse device, it is characterised in that be provided with reaction cell body in the medial surface of described reaction warehouse base, arranges reaction tank at reaction cell body mid portion, and the surrounding at reaction tank is provided with sealing ring；
The mid portion of the lateral surface of described reaction warehouse base is provided with temperature control assembly, and the mid portion at reaction warehouse base is provided with temperature sensor mounting groove, is provided with heating wire mounting groove in the bottom of mounting groove, places heating wire and temperature sensor respectively.
DNA sequencer the most according to claim 8 turning reaction warehouse device, it is characterised in that the surrounding at described temperature sensor mounting groove is provided with four guide post installing holes, and upper and lower sides is provided with self adaptation spring mounting hole；On the cover board it is also equipped with guiding rod aperture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510711551.3A CN105296345B (en) | 2015-10-28 | 2015-10-28 | A kind of DNA sequencer turning reaction warehouse device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510711551.3A CN105296345B (en) | 2015-10-28 | 2015-10-28 | A kind of DNA sequencer turning reaction warehouse device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105296345A CN105296345A (en) | 2016-02-03 |
| CN105296345B true CN105296345B (en) | 2016-09-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510711551.3A Expired - Fee Related CN105296345B (en) | 2015-10-28 | 2015-10-28 | A kind of DNA sequencer turning reaction warehouse device |
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| CN (1) | CN105296345B (en) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703312B (en) * | 2012-05-24 | 2014-03-19 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703311B (en) * | 2012-05-24 | 2013-08-21 | 中国科学院北京基因组研究所 | Reaction bin for adaptive regulation of DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102766574B (en) * | 2012-05-24 | 2013-12-25 | 中国科学院北京基因组研究所 | Reaction chamber for DNA sequenator |
| CN102703302B (en) * | 2012-05-24 | 2013-11-27 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
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2015
- 2015-10-28 CN CN201510711551.3A patent/CN105296345B/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN105296345A (en) | 2016-02-03 |
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