CN105316214B - Box-type DNA sequencer with built-in reaction chip - Google Patents
Box-type DNA sequencer with built-in reaction chip Download PDFInfo
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- CN105316214B CN105316214B CN201510728783.XA CN201510728783A CN105316214B CN 105316214 B CN105316214 B CN 105316214B CN 201510728783 A CN201510728783 A CN 201510728783A CN 105316214 B CN105316214 B CN 105316214B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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Abstract
The invention relates to a box-type DNA sequencer with a built-in reaction chip. A reaction cabin component comprises a turnable cabin body, wherein a reaction chip is arranged in the middle of a reaction chip; a reaction tank body is arranged in the inner side face of a reaction cabin base; a reaction tank is arranged in the middle of the reaction tank body; a seal ring is arranged around the reaction tank; a temperature control component is arranged in the middle of the outer side face of the reaction cabin base; a temperature sensor mounting groove is arranged in the middle of the reaction cabin base; an electric heating wire is arranged in the middle of the reaction cabin base; and the electric heating wire is arranged in an electric heating wire mounting groove of the base. According to the sequencer disclosed by the invention, the reaction chip mounting groove, as well as the electric heating wire and the temperature sensor for keeping the temperature of the reaction chip, is arranged on the reaction cabin base and the reaction chip is fastened therein by virtue of the reaction cabin base and the reaction chip mounting base which are respectively arranged on the upper side and the lower side, the reaction chip is rapid, accurate and stable to be mounted, and the reaction chip is fixed in mounting position and is not easy to become deviated.
Description
Technical field
The field the present invention relates to dna is sequenced, more particularly, to a kind of reaction chip that is airtight, having frame structure is built-in
Box dna sequenator.
Background technology
In dna sequencing technologies field, dna sequence testing chip and sequencing reagent are attached on main frame, by control computer root
Start sequencing program according to module number and position, automation carries out sequencing reaction, the data transfer of generation calculates to data analysis
Machine, after completing sequencing, computation analysis software carries out the work such as image procossing, sequence reading, quality analysis, sequence assembly,
Obtain the sequence information of dna sample eventually.
During dna sequencing, reaction chip only can carry out sequencing reaction after installation that is correct, consolidating,
During actual test, need in real time reaction chip to be changed.General reactions chip is arranged on carrier, reaction warehouse
Generally comprise the two parts interlocking up and down, but when changing reaction chip, two are partially separated difficulty, after chip, instead
Answer chip difficult to reach uniform location, the easy deflection of reaction chip.
In view of drawbacks described above, creator of the present invention passes through long research and practice obtains this creation finally.
Content of the invention
It is an object of the invention to provide a kind of built-in box dna sequenator of reaction chip, in order to overcome above-mentioned skill
Art defect.
For achieving the above object, the present invention provides a kind of reaction chip built-in box dna sequenator, and it includes reacting
Record subsystem, agent delivery system and control system, and carry the sequenator casing of above-mentioned each system, wherein, described
Reaction record subsystem and agent delivery system are separately positioned in the left and right sides interval of sequenator casing；Described control system
System is arranged on the left of casing the rear side of the reaction record subsystem in interval, controls pressing of reaction subsystem and agent delivery system
According to predetermined sequential action；
Described sequenator casing includes body supports assembly, is arranged on the liner board component inside body supports assembly；
Described reaction record subsystem includes reaction warehouse assembly, and described reaction warehouse assembly includes turning warehouse,
The mid portion of reaction chip mounting seat is provided with reaction chip, is provided with cover plate on reaction chip；In reaction warehouse base
It is provided with reaction pond body in medial surface, in reaction pond body mid portion setting reaction tank, the surrounding of reaction tank is provided with sealing
Circle；
The mid portion of the lateral surface of reaction warehouse base is provided with temperature control assembly, sets up separately in the pars intermedia of reaction warehouse base
It is equipped with temperature sensor mounting groove, the bottom of mounting groove is provided with heating wire mounting groove, place heating wire and temperature respectively
Sensor；The middle part of reaction warehouse base is provided with electrical heating wire, it is arranged in the heating wire mounting groove of base；In temperature
Degree sensor and control assembly are also poured and are inoculated with seal.
Further, the two ends in reaction tank are respectively arranged with Reagent Tube and install screwed hole, in order to be connected with Reagent Tube；?
The end that Reagent Tube installs screwed hole is provided with inlet and liquid outlet, and liquid inlet and outlet is communicated with the two ends of reaction tank, in order to
Cycle detection reagent.
Further, described body supports assembly is made up of steel plate splicing, by way of splicing or screw fastening
Fixing；It includes base assembly, top plate member, connects and composes the support frame assembly of casing side frame with base assembly, delays
Flushing system mounting seat；Wherein,
Described support frame assembly includes left side vertical plate assembly, the middle riser assembly separating left and right two tanks, right side
Riser assembly and be arranged on right side riser forward portion right side mend slab, side stand rearways board component, be arranged in top plate member
The first cable fixed seat and the first cable fixed seat, and the connecting plate that each assembly is linked together.
Further, it is additionally provided with a positioning boss in described mounting groove, it is in order to install with temperature control assembly cover plate
Shi Dingwei；
Positioning recess corresponding with positioning boss is provided with temperature control assembly cover plate.
Further, the both sides in described reaction chip are provided with chute pressing plate, in order to be fixed to reaction chip；
A side portion of reaction chip mounting seat is provided with mounting seat support component, it is in order in taking-up or chip placement
When, support described reaction chip mounting seat；It includes torsional-spring supported portion, is arranged in mounting seat, in order to support described torsion
Spring, described torsional-spring supported portion is a recess carrying protruding end.
Further, described motion platform assembly, ccd camera is fed into suitable picture-taking position by it, so that ccd phase
Machine carries out, to the visible optical information producing in reaction chip, collection of taking pictures；Described platform assembly, it supports described anti-
Answer storehouse assembly, motion platform assembly；Described motion control unit, it is controlled in real time to the operation of ccd camera and reaction chip
System, powered motion platform assembly moves to the picture-taking position of ccd camera, detects and control the temperature of reaction warehouse.
Further, described platform assembly includes vibration damper plate and is arranged on the first damping wing of vibration damper plate both sides
Plate and the second damping wing plate, are respectively arranged with dampening assembly in the lower end of two damping wing plates；
After installation is complete, platform assembly is in indentations, and reaction warehouse assembly is arranged on vibration damper plate, two ends respectively with
Two vibration damper plate contacts；
Damping wing plate includes gripper shoe, and it has certain height, supports described reaction warehouse assembly.
Compared with prior art the invention has the benefit that the built-in box dna of the reaction chip of the present invention is sequenced
Instrument, arranges reaction chip mounting groove and the heating wire keeping reaction chip temperature and temperature sensor in reaction warehouse base, instead
Answer chip to be fastened in it by upper and lower reaction warehouse base and reaction chip mounting seat, and, reaction warehouse is built in again and can turn over
Turn in reaction warehouse body, change easy, reaction chip installation is quick, accurate, firm, and reaction chip installation site is fixed, be difficult partially
Tiltedly.
The present invention dna sequencing during, sequenator should have during agent delivery, sequencing reaction continuously, can
The characteristic of control, and, sequencing reaction and agent delivery device should have stable, airtight operating environment；Overall structure is stable, surveys
Sequence efficiency high.
The dna sequenator of the present invention has a robust structure of many recanalization, easy for installation, compact conformation between each structure,
Stability is high.
Reaction warehouse assembly that the present invention is sequenced in dna and the periphery setting casing of reagent assembly are so that reaction warehouse has
The space of enough precision, stability and cleaning；Space is reasonable, be easily portable adjustment.It is empty that casing is provided with left and right two parts
Between, accommodate reaction component and agent delivery, storage assembly respectively, the equipment in the space of left and right works respectively, form flowing water and be turned into
Industry, testing efficiency is high.
The liquid-supplying system of the dna sequenator of the present invention is controlled the storage of reagent, conveying, is selected by control unit；Liquid case
Hoistable platform assembly is moved reagent delivery to specified location by guide rail, easy to control, accurate；Liquid case hoistable platform is by pump valve
Integrated tube assembly is eased up and is rushed liquid case loading/unloading platform assembly and transport up and down, completes reagent conveying, course of reaction.
Brief description
Fig. 1 is the functional block diagram of the dna sequenator of the present invention；
Fig. 2 a is the overall structure diagram of the dna sequenator casing of the present invention；
Fig. 2 b is the first structure schematic diagram of the body supports assembly of dna sequenator casing of the present invention；
Fig. 2 c is the second structural representation of the body supports assembly of dna sequenator casing of the present invention；
Fig. 3 is the overall structure diagram of the dna sequenator reaction record subsystem of the present invention；
Fig. 4 is the first perspective view of the reaction warehouse assembly of the present invention；
Fig. 5 a is the first perspective view of the turning warehouse of the present invention；
Fig. 5 b is the second perspective view of the turning warehouse of the present invention；
Fig. 6 a is the perspective view of the locating piece of the present invention；
Fig. 6 b is the structural representation of the electronic lock of the present invention；
Fig. 6 c is the structural representation of the electronics lock of the present invention；
Fig. 6 d is the cross section structure diagram of the electronic lock of the present invention；
Fig. 7 is the cross section structure diagram of the turning reaction warehouse assembly of the present invention along first lug；
Fig. 8 is the 3rd perspective view of the turning warehouse of the present invention；
Fig. 9 a is the structural representation of the reaction chip assembly of the present invention；
Fig. 9 b is the structural representation of the reaction chip mounting seat of the present invention；
Fig. 9 c is the structural representation of the medial surface of reaction warehouse base of the present invention；
Fig. 9 d is the structural representation of the lateral surface of reaction warehouse base of the present invention；
Fig. 9 e is the structural representation of the temperature control assembly cover plate of the present invention；
Figure 10 a is the cross section structure diagram along liquid inlet and outlet of the reaction chip assembly of the present invention；
Figure 10 b is the cross section structure diagram of the turning reaction warehouse assembly of the present invention along self-adaptative adjustment part；
Figure 11 is the turning reaction warehouse assembly cross section structure diagram of the present invention；
Figure 12 a is the cross section structure diagram of the back shroud of the present invention；
Figure 12 b is the perspective view of the bonnet board component of the present invention；
Figure 12 c is the perspective view of the back shroud of the present invention；
Figure 13 a is the structural representation of the platform assembly of the present invention；
Figure 13 b is the structural representation of the vibration damper plate of the present invention；
Figure 14 is the structural representation of the motion platform assembly of the present invention；
Figure 15 is the cross section structure diagram of the motion platform assembly of the present invention；
Figure 16 a is the perspective view of the liquid case hoistable platform assembly of the present invention；
Figure 16 b is the side structure schematic view of the liquid case hoistable platform assembly of the present invention；
Figure 17 a is the perspective view of the integrated tube assembly of pump valve of the present invention；
Figure 17 b is the overlooking the structure diagram of the integrated tube assembly of pump valve of the present invention；
Figure 18 a is the perspective view of the buffering liquid case loading/unloading platform assembly of the present invention；
Figure 18 b is the positive structure diagram of the buffering liquid case loading/unloading platform assembly of the present invention.
Specific embodiment
Below in conjunction with accompanying drawing, the above-mentioned He other technical characteristic of the present invention and advantage are described in more detail.
Refer to shown in Fig. 1, it is the functional block diagram of the dna sequenator casing of the present invention, the dna of the embodiment of the present invention surveys
Sequence instrument includes reaction record subsystem, agent delivery system and control system, and the sequenator casing of the above-mentioned each system of carrying,
In the present embodiment, described reaction record subsystem and agent delivery system are separately positioned on the left and right sides of sequenator casing
In interval, and connected by the agent delivery pipeline being arranged in casing；Described set-up of control system is interval on the left of casing
The rear side of interior reaction record subsystem, control reaction subsystem and agent delivery system according to predetermined sequential action.
Described control system controls agent delivery system by reagent delivery to reaction record subsystem, reacts record point
It is provided with reaction warehouse assembly, reagent flows through the reaction chip being arranged in reaction warehouse assembly and reacts, reaction record in system
The photo in ccd collected by camera course of reaction in subsystem simultaneously obtains surveyed dna sequence after analysis.
Refer to shown in Fig. 2 a, it is the overall structure diagram of the dna sequenator casing of the present invention, the embodiment of the present invention
Sequenator casing include body supports assembly 21, be arranged on the liner board component of body supports assembly 21 inner side, offside door is total
Become assembly 23, offside door assembly assembly 26, buffer liquid case loading/unloading platform assembly 25, left side cover plate assembly 30, rear side cover plate assembly,
Upper cover plate 3110.
Described liner board component includes left side storehouse interior finishing panel assembly 24, interior finishing panel assembly and right side storehouse are right on the left of the storehouse of right side
Side interior finishing panel assembly 22.
In embodiments of the present invention, casing is divided into left and right two parts, is provided with reaction record subsystem, that is, in the casing of left side
Reaction warehouse assembly and ccd camera；It is provided with agent delivery system in right case, including pump valve pipe integrated unit, liquid case lifting
Platform and display, are provided with control assembly in the rear side of casing.Operationally, pump valve integrated unit by reagent solution according to suitable
Sequence is delivered in reaction warehouse assembly, completes chemical reaction, and under the control of the control unit, ccd camera completes to take pictures, and final warp
Cross data analysis to take pictures result, point reach a conclusion.
Wherein, the bottom of body supports assembly 21 supporting box, sidepiece, the segmentation of two housing area in rear portion and left and right
Portion；The medial support assembly of two housing area in left and right is provided with plaque, plays heat-insulated and increase structural stability
Effect；The front portion of left side casing is provided with offside door assembly assembly 23, when opening offside door assembly assembly, can be to being arranged on
Reaction warehouse assembly in left box body is operated；The right side of right side casing is provided with offside door assembly assembly 26, when opening, can
Pump valve integrated unit is operated, setting various reagents pipe and control valve road in this subregion.
Refer to shown in Fig. 2 b-2c, it is the structural representation of the body supports assembly of the present invention；The embodiment of the present invention
Body supports assembly is made up of steel plate splicing, is fixed by way of splicing or screw fastening；It include base assembly 212,
Top plate member 2112, connects and composes the support frame assembly 211 of casing side frame with base assembly 212, and buffer system is installed
Seat 213.Wherein, described support frame assembly 211 includes left side vertical plate assembly 2116, the centre of separation left and right two tanks is stood
Board component 2115, right side riser assembly 2117 and be arranged on right side riser forward portion right side mend slab 2119, side stand rearways
Board component 2118, the first cable fixed seat 2113 being arranged in top plate member 2112 and the first cable fixed seat 2114,
And the connecting plate 2133 that each assembly links together.
Wherein, described left side vertical plate assembly 2116, middle riser assembly 2115 surround the reaction accommodating reaction warehouse assembly
Storehouse accommodating cavity 200；First cable fixed seat 2114 is arranged on top plate member, left side casing is divided into the anti-of front side by this steel plate
Answer the circuit board accommodating cavity 20 of storehouse accommodating cavity 200 and rear side；Middle riser assembly 2115 and right side riser assembly 2117 surround examination
Agent chamber 2001.The middle steel plate of top plate member is provided with the first bending plate 2141 and the second bending plate 2142, both integrally become
The part that type, respectively steel plate two bending on the side are formed, forms between the first bending plate 2141 and the second bending plate 2142
Wire casing, passes through for wire.
Described buffer system mounting seat 213 includes being separately positioned on left side vertical plate assembly 2116 and middle riser assembly
Platform support panel 2132 on 2115 and Reagent Tube support base 2131, described platform support panel 2132 arranges platform group
Part and reaction warehouse assembly thereon.
Casing of the present invention adopts plate to connect, not only easy for installation, and the structural strength that each assembly links together
Height, can support the weight of reaction warehouse assembly, camera and pump valve integrated unit, and can provide stable, reliable environment.
Refer to shown in Fig. 3, it is the overall structure diagram of the dna sequenator reaction record subsystem of the present invention, this
Reaction record subsystem in inventive embodiments include reaction warehouse assembly 12, motion platform assembly 14, motion control unit 15,
Ccd camera 101 and platform support component 13, wherein,
Described reaction warehouse assembly 12, setting reaction chip assembly in it, in the presence of control system, reactant liquor enters
Carry out chemical reaction in reaction chip, produce visible ray；Described motion platform assembly 14, ccd camera is fed into suitably by it
Picture-taking position, in reaction chip produce visible optical information carry out collection of taking pictures；Described platform assembly 13, its
The described reaction warehouse assembly 12 of support, motion platform assembly 14；Described motion control unit, it is to ccd camera and reaction core
The operation real-time control of piece.
In embodiments of the present invention, described reaction warehouse assembly 12 can be installed, fix and adjust described reaction chip,
Ensure that reaction chip is reacted in stable environment, and, reaction chip can conveniently take out and lock.
Refer to shown in Fig. 4, described reaction warehouse assembly 12 includes being arranged on erecting on described platform assembly 13
The door frame 121 of straight setting, is provided with turning reaction warehouse assembly 124 on the top in the outside of door frame 121, in turning reaction warehouse
The lower end of assembly 124 is connected with turnable basement 122, and turnable basement 122 is fixed on the middle part of door frame 121, turning reaction
Storehouse assembly 124 can rotate around described turnable basement 122, in order to take out or to put into reaction chip.
It is additionally provided with electronics lock set 125 in the left side of the upper end of described door frame 121, only in electronics lock set 125
When opening, turning reaction warehouse assembly 124 can be rotated, reaction chip is operated.In embodiments of the present invention, if
It is equipped with door frame 121, support reaction chip and dependency structure, substantially increase the stability of system.
Turning reaction warehouse assembly 124 includes turning warehouse, is provided with the mid portion of described turning warehouse
Back shroud 1242, in order to encapsulate Reagent Tube；Bottom is provided with the first rotating part, it includes the first accommodating chamber 1243, in order to hold
Receive turnable basement 122, the first accommodating chamber 1243 is arranged on the centre position of bottom；It is to be symmetrical arranged in the first accommodating chamber both sides
First lug 1244, have through hole 1246 in the middle setting of first lug 1244；In order to described turnable basement 122
Connect.Turnable basement rotates in the first accommodating chamber 1243 relative to turning warehouse, has higher stability, ensures simultaneously
The transmission of reagent data is unimpeded.Door frame 121 has the structure of sidepiece baffle plate 1211 for one side, and it is integrally formed.
Refer to shown in Fig. 4, the turnable basement 122 in the embodiment of the present invention has the company of two the second lugs 1221
Connect base, the second lug 1221 is symmetricly set on a side 1223 of base, is provided with through hole 1224 in the middle of it, with institute
The through hole 1246 of the middle setting of first lug 1244 on turning warehouse stated is co-axially mounted, and, the second lug 1221
It is arranged on the inner side of first lug 1244.
Incorporated by reference to shown in Fig. 2, in embodiments of the present invention, the both sides of described turnable basement 122 are arranged on sidepiece gear
In plate 1211 and contact positioning with sidepiece baffle plate 1211；Described first lug 1224 is being co-axially mounted with the second lug 1221
Afterwards, the upper-end contact of end and turnable basement 122, and, the sidepiece of turning warehouse and the sidepiece baffle plate 1211 of door frame 121
Medial surface contact is it is ensured that turning warehouse and turnable basement 122 are located just in sidepiece baffle plate 1211.
Refer to shown in Fig. 5 a and 5b, in the inner side of the centre of described turning warehouse, reaction chip assembly be installed,
Accordingly, the middle setting in turning warehouse has the first cavity 1785 accommodating reagent pipeline, at the bottom of the first cavity 1785
Portion is provided with first boss 1784, and first boss 1784 is in order to carry the support member of described reaction chip assembly, convex first
Platform 1784 is provided with four guide post installing holes 1786, and guide post installing hole 1786 passes through the two ends of turning warehouse, reaction core
Piece assembly and support member, reaction chip assembly is connected with turning warehouse；Set in the upper end of described first boss 1784
It is equipped with second boss 1783, second boss 1784 is in order to position described support member；Set in described second boss 1784 upper end
It is equipped with locating surface 1782, locking screw nail 1787 is provided with locating surface 1782.Described in above-mentioned first cavity body structure positioning
Reaction chip assembly, and, reaction chip assembly in normal work it is impossible to enough rotations, be merely able to move along guide post.
The upper surface of the first accommodating chamber 1243 of described turning warehouse is provided with Reagent Tube installing hole 1788 He
Electric wire installing hole, is provided with pipe clamp 03 in Reagent Tube installing hole 1788, and passing through in pipe clamp 03 has Reagent Tube 02 and electric wire 01.
The dead bolt of the dead bolt 1252 being additionally provided with the sidepiece of described turning warehouse in electronics lock set 125 is installed
Hole；In the presence of locking member, turning warehouse is fixed together with door frame 121.
The upper end in the outside of described turning warehouse is provided with pressing piece 1241, is only being pressed by pressing piece 1241
Pressure setting can make described turning warehouse rotation.In embodiments of the present invention, incorporated by reference to shown in Fig. 8, described pressing
1241 one end of part are provided with opening 1730, one end is provided with the structure of hook, and the upper end-stopping plate of described hook and door frame is even
Connect, when pressing piece 1241 is subject to downward active force, hook is departed from door frame baffle plate, turning reaction warehouse body being capable of relative door
Frame rotates.
In embodiments of the present invention, described pressing piece 1241 is arranged in a positioning block 1762, under locating piece 1762
End is provided with spring 1761, and spring is arranged in spring base 1763, and spring base 1763 is threadably mounted at turning reaction warehouse
In the spring base hole 1781 of intermediate throughholes 1785 upper surface.
Incorporated by reference to shown in Fig. 5 b-5b, locating piece 1762 is arranged on the locating piece installation cavity of turning reaction warehouse upper end
In 1791, it is provided with positioning step 1790 in cavity.Locating piece 1762 in the embodiment of the present invention includes through hole 1794 He
Lower through-hole 1797, one of both passes through described pressing piece 1241；The side of locating piece arrange locating and machining hole 1796 and
Installing hole 1795.The lower end of the through hole installing pressing piece 1241 is provided with spring eye, spring passes through this hole and pressing piece 1241
Bottom contact.
Embodiment of the present invention setting electronics lock set and pressing piece, carry out two to turning reaction warehouse assembly and door frame
Secondary locking, is respectively adopted the automatically controlled mode with frame for movement it is ensured that reaction chip runs in security context, it is to avoid maloperation.
Incorporated by reference to shown in Fig. 6 b and 6c, the electronics lock set 125 of the present invention includes electronics lock and inductive switch 1251, institute
The inductive switch stated is arranged on electronics lock, and electronics lock is arranged on the sidepiece of turning warehouse, in embodiments of the present invention,
Electronics lock includes sidepiece installing plate 1843, offers installing hole 1844 on sidepiece installing plate 1843；Electronic lock is made by peace
Dress hole 1844 and mounting screw are fixed on turning warehouse.It is provided with symmetrical in described sidepiece installing plate 1843 side
First framework 1841 and second framework 1842, leave the sky installing inductive switch 1251 in the centre of the first framework and second framework
Between.
In embodiments of the present invention, described inductive switch 1251 includes being arranged on the framework 1255 of outside, framework 1255
One side on be provided with connection screw thread hole 1256, it is fixing that it passes through attachment screw 1254 with described electronics lock；Described
Attachment screw 1254 passes through lug 1258 and the first framework 1841, second framework 1842 on side, in order to increase electronics lock
Bonding strength.Described inductive switch 1251 is also associated with control circuit 1252, in order to carry out to inductive switch 1251
Control.
Refer to shown in Fig. 6 d, described electronics lock set 125 is arranged on turning warehouse and the sidepiece of door frame 121, lock
Tongue 1253 enters after stretching out and is arranged in the dead bolt hole 1247 of turning warehouse sidepiece, realizes locking；When dead bolt 1253 is in electricity thorn
In the presence of swashing, when retracting in dead bolt hole 1247, described turning warehouse can be rotated.
The use of door frame, is not only able to make the Stability Analysis of Structures of reaction chip assembly, and, also make turning warehouse with
Turnable basement compact conformation, is easy to operate；Ccd camera is more accurate with the positioning of reaction chip.
The bottom of door frame 121 is provided with the first installing hole 1210, in order to be connected with motion platform assembly 14；In door
It is additionally provided with the second installing hole being connected with turnable basement sidepiece in the middle part of frame.
Incorporated by reference to shown in Fig. 7, in embodiments of the present invention, in order to save structure space and ensure the steady of reagent delivery
Fixed, it is provided with reagent in the through hole 1246 of the middle setting of described first lug 1244 and the through hole of the second lug 1221
Pipe first fixation kit, it includes being arranged on pipe joint 1265 in the lug of left side, the first packing ring 1264, the first rotary shaft
1261, and be arranged on right side lug in reagent pipe joint 1266, second packing ring 1267, the second rotary shaft 1269, wherein, institute
The first packing ring 1264 stated and second packing ring 1267 are arranged between turning warehouse and turnable basement, in the present embodiment,
The diameter of the through hole 1246 of the described middle setting of first lug 1244 is less than the diameter of the through hole of the second lug 1221,
Both medial ends form the positioning rib to packing ring positioning, and after installing two packing rings, inside diameter of gasket is just with second
The diameter of the through hole of lug 1221 is identical.Setting positioning rib and packing ring are it is ensured that turning storehouse in embodiments of the present invention
Gapless between body and turnable basement, both good stabilities after combining, will not rock because there is gap, and,
Packing ring increased both contacts area so that stress between the two reduces, extension device service life.
In the inner side of through hole 1246 of the middle setting of first lug 1244 and the inner side of packing ring, the first rotation is installed
Axle 1261 and the second rotary shaft 1269, the structure of two rotary shafts is identical, is the quill shaft with positioning convex ring；In rotary shaft
One end setting positioning convex ring, be provided with connection screw thread hole, in order to the lug connection portion with described turning warehouse
1263 are connected, and accordingly, are provided with the positioning recess positioning described positioning convex ring in the end of described through hole 1246.?
The centre at the both ends of rotary shaft 1269 be recess, inner side recess in order to described pipe joint 1265, reagent pipe joint
1266 install positioning.It is respectively mounted live wire and Reagent Tube 02, its point in pipe joint 1265, reagent pipe joint 1266
Do not lead in reaction chip assembly.Due in the embodiment of the present invention setting can mutual upset assembly, in upset, right for avoiding
Pipe fitting impacts, and installs Reagent Tube in through-holes, substantially increases the security performance of Reagent Tube.
Refer to shown in Fig. 8, in embodiments of the present invention, described reaction chip assembly is arranged on described turning storehouse
The inner side of body.The inner side of reaction chip assembly is provided with the observation portion 1724 contacting with ccd camera, is provided with transparent
Baffle plate, to take pictures to course of reaction.
Refer to shown in Fig. 9 a, the reaction chip assembly of the present invention includes reaction chip mounting seat 127, reaction warehouse base group
Part 126 and the shading positioning component 128 reaction warehouse base assembly 126 and reaction chip mounting seat 127 locked, opens；For
Easily reaction chip can be taken out and close, reaction chip mounting seat 127 can be relative with reaction warehouse base assembly 126
Rotation.
Refer to shown in Fig. 9 b, it is the structural representation of the reaction chip mounting seat of the present invention, installs in reaction chip
The mid portion of seat 127 is provided with reaction chip, is provided with cover plate 1277 on reaction chip；Install in the both sides of reaction chip
There is chute pressing plate 1279, in order to be fixed to reaction chip.A side portion of reaction chip mounting seat 127 is provided with installation
Seat supports assembly, it is in order in taking-up or chip placement, to support described reaction chip mounting seat；It includes torsional-spring supported portion
1273, it is arranged in mounting seat, in order to support described torsion spring, described torsional-spring supported portion 1273 is one with protruding end 1274
Recess, in the described reaction chip mounting seat 127 of rotation, protruding end 1274 contacts with 1261 at first, in the present embodiment,
Described protruding end 1274 is arranged on the middle part of recess and the edge of chip installation position；Structure using mounting seat itself
Reaction chip mounting seat is supported, better reliability is it is ensured that chip can safely remove and place.
Described mounting seat support component is also included torsion spring 1272 and is connected with torsion spring 1272 and is arranged on drawing of torsion spring two ends
Mandrel 1271, described tension axle 1271 is arranged on lug boss 1275, when starting reaction chip mounting seat, described torsion spring
1272 support mounting seats, and finally rely on the positioning of protruding end 1274, realize supporting.
Be additionally provided with the first rotary shaft hole 1276 in the end of described lug boss 1275, in order to reaction warehouse base 1261
Connect.It is additionally provided with locating slot 1278 in the sidepiece of reaction chip mounting seat.
Refer to shown in Fig. 9 c, it is the structural representation of the medial surface of reaction warehouse base of the present invention, at reaction warehouse bottom
It is provided with reaction pond body 1266, in reaction pond body 1266 mid portion setting reaction tank 1267, in reaction tank in the medial surface of seat
1267 surrounding 1706 is provided with sealing ring, prevents reaction reagent from leaking.One end of reaction warehouse base 1261 is provided with raised line
1705, in order to position with the cover plate after installation.The other end is provided with the torsional-spring supported knot corresponding with reaction chip mounting seat
Structure, it includes symmetrically arranged two protruding ends 1268, is provided with the second rotary shaft hole 1269 thereon, and it is with the first rotary shaft
Hole 1276 is coaxial, and rotary shaft sequentially passes through holes；It is additionally provided with region 1260 and the positioning area for torsion spring activity in this end
Domain 1707, is provided with order to will install the screwed hole 1712 of reaction Hatch Board in this region.
Refer to shown in Fig. 9 d, it is the structural representation of the lateral surface of reaction warehouse base of the present invention, at reaction warehouse bottom
The mid portion of the lateral surface of seat is provided with temperature control assembly, the mid portion of reaction warehouse base is provided with temperature sensor and installs
Groove 1262, is provided with heating wire mounting groove 1704 in the bottom of mounting groove 1262, places heating wire and temperature sensor respectively.
It is additionally provided with a positioning boss 1703 in mounting groove, it is in order to position when installing with temperature control assembly cover plate 1263.In temperature control group
Positioning recess 1702 corresponding with positioning boss 1703 is provided with part cover plate 1263.
The surrounding of temperature sensor mounting groove 1262 is provided with four guide post installing holes 1742, is provided with upper and lower sides
Self adaptation spring mounting hole 1741；Cover plate 1263 is also equipped with guide rod aperture 1701.
Shading positioning component mounting groove 1265 is additionally provided with reaction warehouse base, in order to install described shading positioning group
Part 128, is provided with installation base 1744 in it.
Incorporated by reference to shown in Figure 10 a-10b, the middle part of reaction warehouse base is provided with electrical heating wire 1754, it is arranged on
In the heating wire mounting groove 1704 of base；It is provided with TEMP in the temperature sensor mounting groove 1262 on electrical heating wire top
Device 1720 and control assembly, in order to detect reaction tank temperature and according to set threshold value be adjusted；In temperature sensor
1720 and control assembly 1753 also pour and be inoculated with seal 1263, its be encapsulating resin.It is respectively arranged with examination at the two ends of reaction tank
Screwed hole 1721 installed by agent pipe, in order to be connected with Reagent Tube；The end installing screwed hole 1721 in Reagent Tube is provided with inlet
1723 and liquid outlet 1713, liquid inlet and outlet is communicated with the two ends of reaction tank, in order to cycle detection reagent.
The avris of the interior grooves of reaction tank is provided with sealing ring 1713.It is provided with the centre of reaction chip mounting seat
Reaction chip 04, reagent flows through reaction chip and reacts.
In embodiments of the present invention, Reagent Tube 02 passes through the Reagent Tube installing hole 1788 of turning warehouse, is arranged on and can turn over
The middle setting of roll-over body has the first cavity 1785 accommodating reagent pipeline, final coupled reaction pond body, and reagent cartridge is through each
Plant the sealing of cavity, stability is high, is hardly damaged.
It is fixed with back shroud 1242 in the rear side of reaction warehouse, the middle setting of back shroud 1242 has self-adaptative adjustment part
1751, refer to shown in Figure 12 a-12c, have two rows that raised line 1809 is installed in the inner side middle setting of back shroud 1242, installing
The middle setting of raised line 1809 has the first installation base 1812 and the second installation base 1811, offers screw thread on installation base
Hole 1802, self-adaptative adjustment part 1751 is arranged in this screwed hole 1802.
In embodiments of the present invention, described self-adaptative adjustment part 1751 is the end of thread with the connection end of back shroud, and anti-
The connection end answering storehouse assembly is the polished rod 1805 being provided with spring 1806, is provided with nut 1804 between the ends and is positioned,
After installation is complete, nut 1804 is close to installation base.
It is passage 1803 between two described raised lines 1809, enable to Reagent Tube and pass through；Back shroud also sets
It is equipped with the reinforcement gusset 1812 vertical with raised line 1809；Upper surface 1810 bit plane in raised line 1809 and boss；In plate below
The centre of peripheral framework 1807 and corner be provided with trip bolt hole 1801, in order to described turning reaction warehouse even
Connect fixation.
Above-mentioned bonnet board component, is not only able to carry out face sealing to reaction warehouse assembly adjust additionally it is possible to install fixing self adaptation
One integral piece, structure is simple.
In embodiments of the present invention, it is provided with self-adaptative adjustment part between turning reaction warehouse and reaction warehouse base 126
1751, the end spring of self-adaptative adjustment part 1751 and the end contact of reaction warehouse base；When being fed using ccd camera, phase
When machine and reaction chip component touch, when adjusting camera position, can be now, adaptive by using adjusting in the way of movable
Answer adjustment part can change the position of reaction chip assembly it is ensured that having higher accuracy of detection according to the change in location of camera,
Simultaneously, it is to avoid system is caused damage.
Refer to shown in Figure 13 a-13b, the platform assembly of the embodiment of the present invention includes vibration damper plate 1497 and is arranged on
First damping wing plate 1492 of vibration damper plate 1497 both sides and the second damping wing plate 1491, in the lower end of two damping wing plates respectively
It is provided with dampening assembly 1493.After installation is complete, platform assembly is in indentations, and reaction warehouse assembly 12 is arranged on vibration damper plate
On 1497, two ends are contacted with two vibration damper plates respectively.
Damping wing plate includes gripper shoe 1497, and it has certain height, supports described reaction warehouse assembly；Gripper shoe
The middle setting of 1497 integrally formed upper head plates 1495 has the mounting groove 1492 of motion control unit 15, is provided with motion in it
The circuit board of control unit 15 and various circuit；It is reinforcing plate 1496 in the lower end of mounting groove 1492, in order to open up mounting groove
1492.The platform assembly of the embodiment of the present invention can either be symmetrical arranged the stability of guarantee system, can arrange control again
Assembly, saves space, compact conformation.
Motion platform assembly 14 and ccd camera 101 are additionally provided with platform assembly 13, refer to shown in Figure 16,
Described motion platform assembly 14 includes lead screw guide rails assembly, the support lead screw assembly that motor 136 is connected with motor output shaft
Support base and detection components, camera support assembly.
Refer to shown in Figure 14, the output shaft of motor 136 is connected by shaft coupling 1377 with leading screw 137, motor and leading screw
Junction is provided with support base 1361, and the end of leading screw is provided with bearing 1371 and axial positioning sleeve 1376.On leading screw
It is connected with nut 1374, nut is arranged in nut mounting seat 1375；The upper end of nut mounting seat 1375 is with connecting plate 1012 even
Connect, the upper end of connecting plate 1012 is connected with camera mounting seat 1011；In bearing end, bearing friction top 1372 is set.
Described nut mounting seat 1375 is arranged on the slide block 1012 being contacted with guide rail 132, and drives phase on slide block
Machine moves.
The agent delivery system of the embodiment of the present invention includes control unit, buffering liquid case loading/unloading platform assembly, liquid case lifting
The integrated tube assembly of platform assembly, pump valve, wherein, described buffering liquid case loading/unloading platform can load and unload various reagents, and through liquid
Case hoistable platform assembly be transported to the position specified up and down, for described pump valve integrated pipe Component driver, described pump valve is integrated
Tube assembly selects Reagent Tube, the driving through hydraulic pump and the open and close controlling of hydraulic valve needing, by specified reagent delivery extremely
Reacted in reaction warehouse assembly；The action of each element above-mentioned is controlled by control unit unification.
Refer to shown in Figure 16 a-16b, the liquid case hoistable platform assembly of the embodiment of the present invention for motor leading screw drive upper
Lower reciprocating mechanism, it is arranged in the right side casing of sequenator.It is included motor 401 and is connected simultaneously with motor
Support support base 402 lead screw assembly being connected with motor, the reaction the being arranged on sidepiece liquid case lifting seat of motor
408, wherein, in described reaction liquid case lifting seat 408, it is equiped with buffer solution case loading/unloading platform assembly, reaction liquid case lifts
Seat 408 is arranged on the installing plate 404 being connected with reaction liquid case lifting seat, under the driving of screw mechanism, carries reaction and uses
Liquid case lifting seat Reagent Tube group moves up and down.
Reaction liquid case lifting seat 408 includes the intermediate plate 4081 being connected with installing plate, is arranged on the support of intermediate plate both sides
Plate 4082, supporting plate 4082 is provided with horizontal ends, in order to place buffering liquid case loading/unloading platform assembly.
The described output shaft of motor 401 is connected with leading screw 403, and leading screw is arranged in base 405, base 405 with
The end of motor 401 is connected by support base 402；The sidepiece of base 405 is provided with stroke detection switch 406.Described
The output shaft of motor be connected with the connection end 4034 of leading screw by shaft coupling 4035；Nut 4031 is connected with leading screw,
Nut is fixed on nut seat 4032；Bearings are passed through in leading screw lower end, and bearing end is provided with bearing (ball) cover 4011.
The liquid case hoistable platform assembly of the embodiment of the present invention is moved reagent delivery to specified location, controlling party by guide rail
Just, precisely；Integrated for pump valve tube assembly is eased up and is rushed liquid case loading/unloading platform assembly and transport up and down by liquid case hoistable platform, completes reagent defeated
Give, course of reaction.
Refer to Figure 17 a-18b to show, the integrated tube assembly of pump valve of the embodiment of the present invention will by the syringe needle suction pipe 507 of downside
Reagent sucks from the Reagent Tube buffering liquid case loading/unloading platform assembly, and by the selection of fluid path control unit 502, drives control
Valve module 509 processed and hydraulic pump 506 are selected, and reagent is entered in reagent cartridge, carry out sequencing reaction.
The integrated tube assembly of described pump valve includes pump valve integrated base plate 508, and pump valve integrated base plate 508 includes peristaltic pump
Fixed plate that installing plate 5011 is connected with peristaltic pump installing plate 5011, base plate, are arranged on sipper pore group and the bottom on base plate
Control valve unit installing board in the middle of plate；Peristaltic pump installing hole is provided with peristaltic pump installing plate 5011, arranges two thereon
Peristaltic pump 505；Interval setting multiple reagent pipe clamp and wire clamp 5015 are gone back on base plate.Accordingly, control valve assembly 509 is installed
In the mid portion of pump valve integrated base plate 508, hydraulic pump 506 is arranged on its sidepiece, controls single in the other side setting fluid path
Unit 502, fluid path control unit 502 passes through a bending plate 503 and the isolation of fluid path part.
In embodiments of the present invention, in order to carry out sequencing reaction to plurality of reagents, the sipper pore group on base plate is side by side
It is provided with multiple holes, syringe needle suction pipe 507 passes through sipper pore, the other end is connected Reagent Tube and connected with the fluid path port of corresponding control valve
Connect；Sipper pore arranges pipe clamp 5013, prevents syringe needle suction pipe from tilting.
The embodiment of the present invention, using the integrated tube assembly of pump valve, can select to plurality of reagents simultaneously, and according to pre-
The order first arranging carries out sequencing reaction, and testing efficiency is high.
Refer to shown in Figure 18 a-18b, buffering liquid case loading/unloading platform assembly is the structure of drawing and pulling type, and platform sets examination
After agent, whole platform assembly is arranged in the casing of right side, when needing to load and unload reagent, platform is pulled out, after placing, again pushes away
Return original position.
It includes carrier fluid platform 310, and in the present embodiment, it is an arm-tie carrying handle 3101, and shakes hands to fix
On handle plate；The both sides of carrier fluid platform 310 are provided with connecting plate 311, and the horizontal part of connecting plate 311 is lifted with reaction liquid case
The supporting plate of seat 408 connects；In the presence of reaction is with liquid case lifting seat, carrier fluid platform 310 moves up under pump valve integrated pipe
At the syringe needle suction pipe 507 of side, control through control unit, selectively draw reagent.
Slide track component 312 is set between described carrier fluid platform 310 and the connecting plate 311 of both sides, it makes carrier fluid platform
Move along connecting plate.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, and this embodiment is simultaneously not used to
Limit the scope of the claims of the present invention, all equivalence enforcements done without departing from the present invention or change, be intended to be limited solely by the technology of the present invention
In the range of scheme.
Claims (7)
1. a kind of built-in box dna sequenator of reaction chip is it is characterised in that it includes reaction record subsystem, reagent
Supply system and control system, and carry the sequenator casing of above-mentioned each system, wherein, described reaction record subsystem and
The left and right sides that agent delivery system is separately positioned on sequenator casing is interval interior；Described set-up of control system is on the left of casing
Interval in reaction record subsystem rear side, control reaction subsystem and agent delivery system according to predetermined sequential move
Make；
Described sequenator casing includes body supports assembly, is arranged on the liner board component inside body supports assembly；
Described reaction record subsystem includes reaction warehouse assembly, motion platform assembly, ccd camera, motion control unit peace
Platform support component, described reaction warehouse assembly includes turning warehouse, is provided with instead in the mid portion of reaction chip mounting seat
Answer chip, cover plate is provided with reaction chip；It is provided with reaction pond body in the medial surface of reaction warehouse base, in reaction pond body
Mid portion arranges reaction tank, and the surrounding of reaction tank is provided with sealing ring；
The mid portion of the lateral surface of reaction warehouse base is provided with temperature control assembly, is provided with the mid portion of reaction warehouse base
Temperature sensor mounting groove, is provided with heating wire mounting groove in the bottom of mounting groove, places heating wire and TEMP respectively
Device；The middle part of reaction warehouse base is provided with electrical heating wire, it is arranged in the heating wire mounting groove of base；Pass in temperature
Sensor and control assembly are also poured and are inoculated with seal.
2. the built-in box dna sequenator of reaction chip according to claim 1 is it is characterised in that in reaction tank
Two ends are respectively arranged with Reagent Tube and install screwed hole, in order to be connected with Reagent Tube；The end setting of screwed hole is installed in Reagent Tube
There are inlet and liquid outlet, liquid inlet and outlet is communicated with the two ends of reaction tank, in order to cycle detection reagent.
3. the built-in box dna sequenator of reaction chip according to claim 2 is it is characterised in that described main body
Support component is made up of steel plate splicing, is fixed by way of splicing or screw fastening；It includes base assembly, top board group
Part, connects and composes the support frame assembly of casing side frame, buffer system mounting seat with base assembly；Wherein,
Described support frame assembly includes left side vertical plate assembly, the middle riser assembly separating left and right two tanks, right side riser
Assembly and be arranged on right side riser forward portion right side mend slab, side stand rearways board component, be arranged in top plate member the
One cable fixed seat and the first cable fixed seat, and the connecting plate that each assembly is linked together.
4. the built-in box dna sequenator of reaction chip according to claim 2 is it is characterised in that in described peace
It is additionally provided with a positioning boss, it is in order to position when installing with temperature control assembly cover plate in tankage；
Positioning recess corresponding with positioning boss is provided with temperature control assembly cover plate.
5. the built-in box dna sequenator of reaction chip according to claim 2 is it is characterised in that described anti-
The both sides answering chip are provided with chute pressing plate, in order to be fixed to reaction chip；
A side portion of reaction chip mounting seat is provided with mounting seat support component, its in order in taking-up or chip placement,
Support described reaction chip mounting seat；It includes torsional-spring supported portion, is arranged in mounting seat, in order to support described torsion spring,
Described torsional-spring supported portion is a recess carrying protruding end.
6. the built-in box dna sequenator of reaction chip according to claim 5 is it is characterised in that described motion
Platform assembly, ccd camera is fed into suitable picture-taking position by it, so that ccd camera is to the visible ray producing in reaction chip
Information carries out collection of taking pictures；Described platform assembly, it supports described reaction warehouse assembly, motion platform assembly；Described
Motion control unit, its operation real-time control to ccd camera and reaction chip, powered motion platform assembly moves to
The picture-taking position of ccd camera, detects and controls the temperature of reaction warehouse.
7. the built-in box dna sequenator of reaction chip according to claim 6 is it is characterised in that described platform
Support component includes vibration damper plate and is arranged on the first damping wing plate and the second damping wing plate of vibration damper plate both sides, in two dampings
The lower end of wing plate is respectively arranged with dampening assembly；
After installation is complete, platform assembly is in indentations, and reaction warehouse assembly is arranged on vibration damper plate, two ends respectively with two
Vibration damper plate contacts；
Damping wing plate includes gripper shoe, and it has certain height, supports described reaction warehouse assembly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510728783.XA CN105316214B (en) | 2015-10-30 | 2015-10-30 | Box-type DNA sequencer with built-in reaction chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510728783.XA CN105316214B (en) | 2015-10-30 | 2015-10-30 | Box-type DNA sequencer with built-in reaction chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105316214A CN105316214A (en) | 2016-02-10 |
| CN105316214B true CN105316214B (en) | 2017-01-25 |
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ID=55244540
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510728783.XA Expired - Fee Related CN105316214B (en) | 2015-10-30 | 2015-10-30 | Box-type DNA sequencer with built-in reaction chip |
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| Country | Link |
|---|---|
| CN (1) | CN105316214B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020034143A1 (en) * | 2018-08-16 | 2020-02-20 | 深圳华大智造科技有限公司 | Handle device, positioning device, loading device and gene sequencer |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703302B (en) * | 2012-05-24 | 2013-11-27 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
| CN102703311B (en) * | 2012-05-24 | 2013-08-21 | 中国科学院北京基因组研究所 | Reaction bin for adaptive regulation of DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102766574B (en) * | 2012-05-24 | 2013-12-25 | 中国科学院北京基因组研究所 | Reaction chamber for DNA sequenator |
| CN102703314B (en) * | 2012-05-24 | 2013-09-04 | 中国科学院北京基因组研究所 | Control system for DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703312B (en) * | 2012-05-24 | 2014-03-19 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
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2015
- 2015-10-30 CN CN201510728783.XA patent/CN105316214B/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN105316214A (en) | 2016-02-10 |
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Granted publication date: 20170125 Termination date: 20191030 |