CN105273983B - A kind of PCR disks positioner - Google Patents
A kind of PCR disks positioner Download PDFInfo
- Publication number
- CN105273983B CN105273983B CN201510764788.8A CN201510764788A CN105273983B CN 105273983 B CN105273983 B CN 105273983B CN 201510764788 A CN201510764788 A CN 201510764788A CN 105273983 B CN105273983 B CN 105273983B
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- China
- Prior art keywords
- pcr
- positioning module
- plate
- seed
- cover plate
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to automation equipment field, it is related to the pallet automatic positioning equipment used in seed sample automatic collecting device, specifically related to a kind of PCR disks lifting-positioning device, including frame, the frame is provided with cover plate, the cover plate is provided with some fixed guide poles, the fixed guide pole is provided with liftable mounting plate, the cover plate is provided with lift cylinder, the piston rod of the lift cylinder is connected with liftable mounting plate, the liftable mounting plate is provided with the first positioning module, first positioning module is provided with the second positioning module, first positioning module and the second positioning module right angle setting, second positioning module is provided with PCR pan arrest plates.Simple structure of the present invention, reasonable in design, accurate positioning, seed sample can be made to fall into PCR disk specified locations after cutting, realize seed sample automatic collection function, effectively increase the efficiency of seed sampling, the device have high degree of automation, it is small to the damage of seed the features such as.
Description
Technical field
The invention belongs to automation equipment field, it is related to the pallet used in seed sample automatic collecting device to be automatically positioned
A kind of device, and in particular to PCR disks positioner.
Background technology
Current molecular breeding technology is to carry out genotyping by gathering the blade of seedling, by proportion of crop planting season
Limitation, and need a large amount of plantation spaces, time and manpower, it is impossible to ensure to find optimal plant.If directly carried out to seed
Minimally invasive sampling, cutting a small amount of endosperm carries out DNA extractions and genotyping, the germination activity without damaging seed germ, then
According to genotyping result preferred seed, planted, hybridized and further offspring analysis, will solve this problem.So
And, the sampling and genotyping of up to a million seeds are difficult to complete by artificial means.Accordingly, it would be desirable to develop automated seed sampling
Machine, including automatic dispenser, seed automatic conveying device, seed automatic grabbing device, seed automatic cutting device, kind increment
Product automatic collecting device etc., position is fixed when being worked due to seed automatic cutting device, and seed can only be cut in specified location
Cut, and seed sample automatic collecting device needs to make the seed sample under cutting to fall into PCR disks each specified locations, to carry out
DNA analysis, therefore a kind of PCR disks positioner is developed, it is adapted with seed automatic cutting device, realize that seed sample is collected automatically
Functional meaning is great.
The content of the invention
It is an object of the invention to provide a kind of PCR disks positioner, the device can coordinate seed automatic cutting device, make
PCR disk specified locations are fallen into after obtaining seed sample cutting.
To reach above-mentioned purpose, technical scheme is as follows：
A kind of PCR disks positioner, including frame, the frame are provided with cover plate, and the cover plate is provided with some fixations
Guide post, the fixed guide pole is provided with liftable mounting plate, and the cover plate is provided with lift cylinder, the work of the lift cylinder
Stopper rod is connected with liftable mounting plate, and the liftable mounting plate is provided with the first positioning module, and first positioning module is provided with
Second positioning module, first positioning module and the second positioning module right angle setting, second positioning module are provided with PCR
Pan arrest plate.
Used as further optimization, the cover plate is provided with buffering mounting seat, and the buffering mounting seat is provided with buffering and installs
Plate, the buffering installing plate is provided with oil buffer.
Used as further optimization, the quantity of the fixed guide pole is 4, and the fixed guide pole is respectively arranged at two ends with leads
To bar fixed seat and guide holder, the guide post fixed seat is fixed on the cover board, and the guide holder is fixed on liftable mounting plate.
Used as further optimization, the PCR pan arrests plate is provided with clamping cylinder mounting seat, in the clamping cylinder mounting seat
Clamping cylinder.
Used as further optimization, the bottom of the frame is provided with 4 adjustable lower margins.
Beneficial effects of the present invention are as follows：
A kind of PCR disks positioner that the present invention is provided, simple structure is reasonable in design, and accurate positioning can make seed sample
PCR disk specified locations are fallen into after product cutting, seed sample automatic collection function is realized, the effect of seed sampling is effectively increased
Rate, the device have high degree of automation, it is small to the damage of seed the features such as.
Brief description of the drawings
Fig. 1 is a kind of PCR disks positioning device structure schematic diagram of the invention.
In figure：1- frames, 2- cover plates, 3- lift cylinders, 4- fixed guide poles, 5- guide posts fixed seat, 6- liftings are installed
Plate, 7- guide holders, 8- bufferings mounting seat, 9- bufferings installing plate, 10- oil buffers, the positioning modules of 11- first, 12- second determine
The adjustable lower margin of position module, 13- clamping cylinders, 14- clamping cylinders mounting seat, 15-PCR pan arrests plate, 16-PCR disks, 17-.
Specific embodiment
The present invention is further elaborated below in conjunction with the accompanying drawings.
As shown in figure 1, a kind of PCR disks positioner of the present invention, including frame 1, the frame 1 is provided with cover plate
2, the cover plate 2 is provided with 4 fixed guide poles 4, and the fixed guide pole 4 is provided with liftable mounting plate 6, the fixed guide
Bar 4 is respectively arranged at two ends with guide post fixed seat 5 and guide holder 7, and the guide post fixed seat 5 is fixed on cover plate 2, the guiding
Seat 7 is fixed on liftable mounting plate 6, and the cover plate 2 is provided with lift cylinder 3, and piston rod and the lifting of the lift cylinder 3 are pacified
Dress plate 6 is connected, and the liftable mounting plate 6 is provided with the first positioning module 11, and first positioning module 11 is provided with second to be determined
Position module 12, the right angle setting of 11 and second positioning module of the first positioning module 12, first positioning module 11 and second
Positioning module 12 all drives leading screw to rotate by servomotor, drives guide rail slide block to move back and forth by feed screw nut, and described second
Positioning module 12 is provided with PCR pan arrests plate 15, and the PCR pan arrests plate 15 is formed around spacing flange, for being carried out to PCR disks 16
Spacing and clamping, the cover plate 2 is provided with buffering mounting seat 8, and the buffering mounting seat 8 is provided with buffering installing plate 9, described slow
Rush installing plate 9 and be provided with oil buffer 10, the PCR pan arrests plate 15 is provided with 2 clamping cylinder mounting seats 14, the clamping
Cylinder mounting seat 14 is located at the adjacent two side centre position of PCR pan arrests plate 15 respectively, is all set in the clamping cylinder mounting seat 14
There is clamping cylinder 13, the bottom of the frame 1 is provided with 4 adjustable lower margins 17, and PCR disks highly suitably can be adjusted.
Operation principle of the invention：When a kind of PCR disks positioner of the present invention works, feeding manipulator puts PCR disks 16
To PCR pan arrests plate 15, the piston rod of subsequent clamping cylinder 13 stretches out, PCR disks 16 are clamped with it is spacing, by PCR disks 16
The specified location of PCR pan arrests plate 15 is fixed on, the piston rod of lift cylinder 3 is stretched out, and PCR disks 16 are raised up into specified altitude assignment；Due to
Seed automatic cutting device position is fixed, and seed can only be cut in specified location, and seed automatic grabbing device is by first
Seed delivers to specified location, and the first positioning module 11 and the second positioning module 12 are moved specifies cell as specified by PCR disks 16
Position, now PCR disks 16 specify cell to be placed exactly in immediately below first seed, then seed automatic cutting device cutting kind
Son, the seed sample under being cut is fallen under gravity into during PCR disks 16 immediately below it specify cell；Afterwards, seed is automatic
Next seed is delivered to specified location by grabbing device, and the first positioning module 11 and the second positioning module 12 are moved PCR disks 16
As for immediately below seed, second seed of collection cuts sample to next cell, the like, until in all cells of PCR disks 16
All it is collected into respective seed sample.
It should be appreciated that specific embodiment described above is only used for explaining the present invention, it is not intended to limit the present invention.By
Obvious change that spirit of the invention is extended out or among changing still in protection scope of the present invention.
Claims (5)
1. a kind of PCR disks positioner, it is characterised in that：Including frame (1), the frame (1) is provided with cover plate (2), described
Cover plate (2) is provided with some fixed guide poles (4), and the fixed guide pole (4) is provided with liftable mounting plate (6), the cover plate
(2) lift cylinder (3) is provided with, the piston rod of the lift cylinder (3) is connected with liftable mounting plate (6), the lifting is installed
Plate (6) is provided with the first positioning module (11), and first positioning module (11) is provided with the second positioning module (12), described
One positioning module (11) and the second positioning module (12) right angle setting, second positioning module (12) are provided with PCR pan arrest plates
(15)。
2. a kind of PCR disks positioner according to claim 1, it is characterised in that：The cover plate (2) is provided with buffering peace
Dress seat (8), buffering mounting seat (8) is provided with buffering installing plate (9), and buffering installing plate (9) is provided with oleo cushion
Device (10).
3. a kind of PCR disks positioner according to claim 1, it is characterised in that：The quantity of the fixed guide pole (4)
It it is 4, the fixed guide pole (4) is respectively arranged at two ends with guide post fixed seat (5) and guide holder (7), the guide post is fixed
Seat (5) is fixed on cover plate (2), and the guide holder (7) is fixed on liftable mounting plate (6).
4. a kind of PCR disks positioner according to claim 1, it is characterised in that：The PCR pan arrests plate (15) is provided with
Clamping cylinder mounting seat (14), clamping cylinder (13) on the clamping cylinder mounting seat (14).
5. a kind of PCR disks positioner according to claim 1, it is characterised in that：Frame (1) bottom is provided with 4
Adjustable lower margin (17).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510764788.8A CN105273983B (en) | 2015-11-10 | 2015-11-10 | A kind of PCR disks positioner |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510764788.8A CN105273983B (en) | 2015-11-10 | 2015-11-10 | A kind of PCR disks positioner |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105273983A CN105273983A (en) | 2016-01-27 |
| CN105273983B true CN105273983B (en) | 2017-06-16 |
Family
ID=55143827
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510764788.8A Active CN105273983B (en) | 2015-11-10 | 2015-11-10 | A kind of PCR disks positioner |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105273983B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102124906A (en) * | 2010-12-03 | 2011-07-20 | 浙江理工大学 | Vision-based to-be-grafted seedling sorting device of vegetable and fruit grafting machine |
| CN103609342A (en) * | 2013-11-26 | 2014-03-05 | 浙江大学 | Potted tray vegetable automatic needle grafting type grafting device |
| CN204466334U (en) * | 2015-01-27 | 2015-07-15 | 魏鲁红 | A kind of clamping topping machanism of grafting machinery |
| CN205133586U (en) * | 2015-11-10 | 2016-04-06 | 中国科学院合肥物质科学研究院 | PCR coils positioner |
-
2015
- 2015-11-10 CN CN201510764788.8A patent/CN105273983B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102124906A (en) * | 2010-12-03 | 2011-07-20 | 浙江理工大学 | Vision-based to-be-grafted seedling sorting device of vegetable and fruit grafting machine |
| CN103609342A (en) * | 2013-11-26 | 2014-03-05 | 浙江大学 | Potted tray vegetable automatic needle grafting type grafting device |
| CN204466334U (en) * | 2015-01-27 | 2015-07-15 | 魏鲁红 | A kind of clamping topping machanism of grafting machinery |
| CN205133586U (en) * | 2015-11-10 | 2016-04-06 | 中国科学院合肥物质科学研究院 | PCR coils positioner |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105273983A (en) | 2016-01-27 |
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| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190313 Address after: 230000 No. 350 Shushan Lake Road, Hefei City, Anhui Province Patentee after: HEFEI INSTITUTES OF PHYSICAL SCIENCE, CHINESE ACADEMY OF SCIENCES Address before: 230000 No. 350 Shushan Lake Road, Shushan District, Hefei City, Anhui Province Co-patentee before: Institute of Advanced Manufacturing Technology Patentee before: HEFEI INSTITUTES OF PHYSICAL SCIENCE, CHINESE ACADEMY OF SCIENCES |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230926 Address after: Room 1004, Building 2, Zhongzheng International Plaza, No. 948 Fuyang North Road, Luyang District, Hefei City, Anhui Province, 230001 Patentee after: Zhongke Kezheng Automation Engineering Co.,Ltd. Address before: 230000 No. 350 Shushan Lake Road, Hefei City, Anhui Province Patentee before: HEFEI INSTITUTES OF PHYSICAL SCIENCE, CHINESE ACADEMY OF SCIENCES |
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| TR01 | Transfer of patent right |