CN105255962A - Method for extracting blood sugar-reducing panaxan by enzyme method and application of obtained panaxan - Google Patents
Method for extracting blood sugar-reducing panaxan by enzyme method and application of obtained panaxan Download PDFInfo
- Publication number
- CN105255962A CN105255962A CN201510718039.1A CN201510718039A CN105255962A CN 105255962 A CN105255962 A CN 105255962A CN 201510718039 A CN201510718039 A CN 201510718039A CN 105255962 A CN105255962 A CN 105255962A
- Authority
- CN
- China
- Prior art keywords
- panaxan
- hypoglycemic
- steam
- enzymolysis
- enzymatic extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a method for extracting a blood sugar-reducing panaxan by an enzyme method and the application of the obtained panaxan. The method comprises the following steps of after slicing clean gen-seng into slices with the thickness of 5mm, performing steam explosion treatment on the slices, and drying the slices to obtain gen-seng powder; placing the obtained gen-seng powder in an enzymatic vessel, adding biologic composite enzyme preparations containing mixed protease, pectinase, glucose amylase, lignin-degrading enzyme and laccase into the vessel, and performing enzymolysis for 3 to 5 hours under the action of a high-pressure pulse field; heating the solution obtained after enzymolysis for 5 to 7 minutes at the temperature of 100 DEG C to passivate the solution, adding an organic solvent into the solution to leach for 2 to 3 times, performing centrifugal separation, and after mixing the supernatants, performing spray drying to obtain powder, wherein the drying temperature is not more than 65 DEG C, and the water content is controlled to be less than 7 percent. Due to the fact that in an enzymolysis reaction process, a steam explosion treatment technology and the high-pressure pulse field are introduced, the activity of the enzyme preparation is improved, the enzymolysis reaction speed is increased, and dissolution of saponin substances in the gen-seng is quickly and effectively promoted.
Description
Technical field
The present invention relates to Polyose extraction and applied technical field, be specifically related to the method for the hypoglycemic panaxan of a kind of Enzymatic Extraction and the application of gained polysaccharide thereof.
Background technology
Ginseng (PanaxginsengC.A.Mey) is the dry root of Araliaceae (Araliaceae) plant ginseng, be commonly called as people's rank, ghost lid, smart, Radix Ginseng etc., be the famous and precious medicinal material of China, have the medicinal history of thousands of years.Ginseng is flat, sweet, the micro-hardship of taste and tepor, and have and reinforce vital energy, multiple arteries and veins takes off admittedly, reinforces the spleen to benefit the lung, promotes the production of body fluid to quench thirst, the effect of intelligence development of calming the nerves.In recent years, Chinese scholars has carried out pharmacological research, clinical observation and epidemic investigation to the effective constituent of ginseng, and result shows that ginseng has and improves microcirculation, improves and organize the multiple biological activity such as anti-anoxia ability, anticoagulant, antitumor, anti-ageing, radioprotective, antifatigue.
Containing Multiple components activeconstituentss such as ginsenoside, volatile oil, organic acid, ester class and polyoses in ginseng, wherein ginsenoside is the main active ingredient of ginseng.At present, from ginseng, at least isolated more than 40 and planted ginsenoside monomer.
Ginsenoside to be connected with glycosyl the plant sugar glycoside material formed by aglycon, is extensively present in the root of ginseng, stem, leaf, Hua Zhong.Ginsenoside all belongs to triterpenoid saponin, can be divided into three types: oleanolic acid type saponin is as Ro by its sapogenin structure difference; Protopanaxadiol-type's saponin(e; Protopanaxatriol's type saponin(e.Rear two classes all belong to the tetracyclic triterpene saponin(e of dammarane type, are one of main active ingredient of ginseng.
At present, the traditional extraction process of ginsenoside comprises organic solvent extraction, heating reflux method, soxhlet extraction and steam distillation etc.But there is the problems such as extracting cycle is long, solvent load is high, loss of effective components is large, energy consumption is high.
Summary of the invention
For solving the problem, the invention provides the method for the hypoglycemic panaxan of a kind of Enzymatic Extraction and the application of gained polysaccharide thereof, the quick-fried treatment technology of vapour and pulsed electric field is introduced in enzyme digestion reaction process, improve zymin activity, accelerate enzyme digestion reaction speed, promote total saponins material stripping in ginseng fast and efficiently.
For achieving the above object, the technical scheme that the present invention takes is:
The hypoglycemic panaxan's of an Enzymatic Extraction method, comprises the steps:
S1, get after clean ginseng is cut into the sheet of 5 mm of thickness, be placed in steam-explosion jar, first passing into nitrogen to steam-explosion jar internal pressure is 0.6 ~ 1.4MPa, explosion treatment 7 ~ 23min; Then passing into rapidly steam to steam-explosion jar internal pressure is 1.4 ~ 1.8MPa, Steam explosion treatment 0.6 ~ 2.6min, dry, obtains Ginseng Root Powder;
S2, the Ginseng Root Powder of step S1 gained is placed in enzymatic vessel, the compound bio-enzyme preparation comprising mixing protease, polygalacturonase, glucoamylase, lignin-degrading enzymes, laccase is added in the ratios of 70.0 ~ 100.0 milliliters/ton, 30 ~ 40 DEG C, enzymolysis 3 ~ 5 hours under high-voltage pulse electric field action, strength of electric field 25 ~ 35kv/cm, umber of pulse 6 ~ 10;
S3, by the solution after enzymolysis 100 DEG C heating 5 ~ 7min, make its passivation, add the organic solvent lixiviate 2 ~ 3 times that concentration is 65 ~ 80%, centrifugation, merge supernatant liquor;
S4, the supernatant liquor of gained is carried out spraying dry, drying temperature is no more than 65 DEG C, and moisture controlled, below 7%, obtains powder.
Preferably, described mixing protease comprises Sumizyme MP 35% ~ 39%, neutral protease 37% ~ 41% and flavor protease 20% ~ 28%.
Preferably, described lignin-degrading enzymes is the mixture of peroxidase and fungal laccase.
Preferably, the mass ratio of described proteolytic enzyme, polygalacturonase, glucoamylase, lignin-degrading enzymes, laccase is 3: 2: 1: 2: 1.
Preferably, in described step S3, organic solvent extraction temperature is 70 ~ 85 DEG C, and each time is 0.5 ~ 1.5 hour.
Preferably, described organic solvent is the turpentine derivatives of natural origin.
Preferably, described turpentine derivatives is isomery, the disproportionation products of firpene.
Above-mentioned preparation income earner gracilis polysaccharide can make oral preparations, injection, mucosal absorption, Transdermal absorption formulation.
The present invention has following beneficial effect:
Adopt steam explosion technology to be pulverized by ginseng, diameter of aspirin particle is less, and its surface-area is larger, contributes to the stripping of effective ingredient, and simultaneously due to the broken wall of crude drug cell, in cell, each effective constituents is all in release conditions, shortens extraction time.Adopt the xylogen that lignin-degrading enzymes can be degraded in ginseng, promote the release of activeconstituents in plant, further raising extraction efficiency, biological enzyme technology is adopted to be combined with pulsed electric field, improve zymin activity, accelerate enzyme digestion reaction speed, promote total saponins material stripping in ginseng fast and efficiently.
Embodiment
In order to make objects and advantages of the present invention clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Embodiment
In following examples, the mixing protease used comprises Sumizyme MP 35% ~ 39%, neutral protease 37% ~ 41% and flavor protease 20% ~ 28%, lignin-degrading enzymes is peroxidase and fungal laccase 1: 1 mixing gained in mass ratio, in the compound enzymic preparation used, the mass ratio of proteolytic enzyme, polygalacturonase, glucoamylase, lignin-degrading enzymes, laccase is 3: 2: 1: 2: 1, the isomery that the turpentine derivatives used is firpene, disproportionation products.
Embodiment 1
S1, get after clean ginseng is cut into the sheet of 5 mm of thickness, be placed in steam-explosion jar, first passing into nitrogen to steam-explosion jar internal pressure is 1MPa, explosion treatment 15min; Then passing into rapidly steam to steam-explosion jar internal pressure is 1.6MPa, Steam explosion treatment 1.6min, dry, obtains Ginseng Root Powder;
S2, the Ginseng Root Powder of step S1 gained is placed in enzymatic vessel, the compound bio-enzyme preparation comprising mixing protease, polygalacturonase, glucoamylase, lignin-degrading enzymes, laccase is added in the ratios of 85.0 milliliters/ton, 35 DEG C, enzymolysis 4 hours under high-voltage pulse electric field action, strength of electric field 30kv/cm, umber of pulse 8;
S3, by the solution after enzymolysis 100 DEG C heating 6min, make its passivation, add the turpentine derivatives lixiviate 2.5 times that concentration is the natural origin of 72.5%, extraction temperature is 77.5 DEG C, and each time is 1 hour, centrifugation, merges supernatant liquor;
S4, the supernatant liquor of gained is carried out spraying dry, drying temperature is no more than 65 DEG C, and moisture controlled, below 7%, obtains powder.
Embodiment 2
S1, get after clean ginseng is cut into the sheet of 5 mm of thickness, be placed in steam-explosion jar, first passing into nitrogen to steam-explosion jar internal pressure is 0.6MPa, explosion treatment 7min; Then passing into rapidly steam to steam-explosion jar internal pressure is 1.4MPa, Steam explosion treatment 0.6min, dry, obtains Ginseng Root Powder;
S2, the Ginseng Root Powder of step S1 gained is placed in enzymatic vessel, the compound bio-enzyme preparation comprising mixing protease, polygalacturonase, glucoamylase, lignin-degrading enzymes, laccase is added in the ratios of 70.0 milliliters/ton, 30 DEG C, enzymolysis 3 ~ 5 hours under high-voltage pulse electric field action, strength of electric field 25kv/cm, umber of pulse 6;
S3, by the solution after enzymolysis 100 DEG C heating 5min, make its passivation, add the turpentine derivatives lixiviate 2 times that concentration is the natural origin of 65%, extraction temperature is 70 DEG C, and each time is 0.5 hour, centrifugation, merges supernatant liquor;
S4, the supernatant liquor of gained is carried out spraying dry, drying temperature is no more than 65 DEG C, and moisture controlled, below 7%, obtains powder.
Embodiment 3
S1, get after clean ginseng is cut into the sheet of 5 mm of thickness, be placed in steam-explosion jar, first passing into nitrogen to steam-explosion jar internal pressure is 1.4MPa, explosion treatment 23min; Then passing into rapidly steam to steam-explosion jar internal pressure is 1.8MPa, Steam explosion treatment 2.6min, dry, obtains Ginseng Root Powder;
S2, the Ginseng Root Powder of step S1 gained is placed in enzymatic vessel, the compound bio-enzyme preparation comprising mixing protease, polygalacturonase, glucoamylase, lignin-degrading enzymes, laccase is added in the ratios of 100.0 milliliters/ton, 40 DEG C, enzymolysis 5 hours under high-voltage pulse electric field action, strength of electric field 35kv/cm, umber of pulse 10;
S3, by the solution after enzymolysis 100 DEG C heating 7min, make its passivation, add the turpentine derivatives lixiviate 3 times that concentration is the natural origin of 80%, extraction temperature is 85 DEG C, and each time is 1.5 hours, centrifugation, merges supernatant liquor;
S4, the supernatant liquor of gained is carried out spraying dry, drying temperature is no more than 65 DEG C, and moisture controlled, below 7%, obtains powder.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (8)
1. the hypoglycemic panaxan's of an Enzymatic Extraction method, is characterized in that, comprise the steps:
S1, get after clean ginseng is cut into the sheet of 5 mm of thickness, be placed in steam-explosion jar, first passing into nitrogen to steam-explosion jar internal pressure is 0.6 ~ 1.4MPa, explosion treatment 7 ~ 23min; Then passing into rapidly steam to steam-explosion jar internal pressure is 1.4 ~ 1.8MPa, Steam explosion treatment 0.6 ~ 2.6min, dry, obtains Ginseng Root Powder;
S2, the Ginseng Root Powder of step S1 gained is placed in enzymatic vessel, the compound bio-enzyme preparation comprising mixing protease, polygalacturonase, glucoamylase, lignin-degrading enzymes, laccase is added in the ratios of 70.0 ~ 100.0 milliliters/ton, 30 ~ 40 DEG C, enzymolysis 3 ~ 5 hours under high-voltage pulse electric field action, strength of electric field 25 ~ 35kv/cm, umber of pulse 6 ~ 10;
S3, by the solution after enzymolysis 100 DEG C heating 5 ~ 7min, make its passivation, add the organic solvent lixiviate 2 ~ 3 times that concentration is 65 ~ 80%, centrifugation, merge supernatant liquor;
S4, the supernatant liquor of gained is carried out spraying dry, drying temperature is no more than 65 DEG C, and moisture controlled, below 7%, obtains powder.
2. the method for the hypoglycemic panaxan of a kind of Enzymatic Extraction according to claim 1, is characterized in that, described mixing protease comprises Sumizyme MP 35% ~ 39%, neutral protease 37% ~ 41% and flavor protease 20% ~ 28%.
3. the method for the hypoglycemic panaxan of a kind of Enzymatic Extraction according to claim 1, is characterized in that, described lignin-degrading enzymes is the mixture of peroxidase and fungal laccase.
4. the method for the hypoglycemic panaxan of a kind of Enzymatic Extraction according to claim 1, is characterized in that, the mass ratio of described proteolytic enzyme, polygalacturonase, glucoamylase, lignin-degrading enzymes, laccase is 3: 2: 1: 2: 1.
5. the method for the hypoglycemic panaxan of a kind of Enzymatic Extraction according to claim 1, is characterized in that, in described step S3, organic solvent extraction temperature is 70 ~ 85 DEG C, and each time is 0.5 ~ 1.5 hour.
6. the method for the hypoglycemic panaxan of a kind of Enzymatic Extraction according to claim 1, is characterized in that, described organic solvent is the turpentine derivatives of natural origin.
7. the method for the hypoglycemic panaxan of a kind of Enzymatic Extraction according to claim 6, is characterized in that, described turpentine derivatives is isomery, the disproportionation products of firpene.
8. the application of the panaxan that the method for the hypoglycemic panaxan of a kind of Enzymatic Extraction prepares according to right 1, it is characterized in that, income earner's gracilis polysaccharide can make oral preparations, injection, mucosal absorption, Transdermal absorption formulation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510718039.1A CN105255962A (en) | 2015-10-23 | 2015-10-23 | Method for extracting blood sugar-reducing panaxan by enzyme method and application of obtained panaxan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510718039.1A CN105255962A (en) | 2015-10-23 | 2015-10-23 | Method for extracting blood sugar-reducing panaxan by enzyme method and application of obtained panaxan |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105255962A true CN105255962A (en) | 2016-01-20 |
Family
ID=55095889
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510718039.1A Pending CN105255962A (en) | 2015-10-23 | 2015-10-23 | Method for extracting blood sugar-reducing panaxan by enzyme method and application of obtained panaxan |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105255962A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105582220A (en) * | 2016-02-06 | 2016-05-18 | 济南市第三人民医院 | Preparation method of traditional Chinese medicine for treating diabetic nephropathy |
| CN105768106A (en) * | 2016-03-23 | 2016-07-20 | 北京东方兴企食品工业技术有限公司 | Liver nutrition improving functional food |
| CN108085129A (en) * | 2018-02-09 | 2018-05-29 | 王忠良 | A kind of wood wax oil preparation method |
| CN111269330A (en) * | 2020-04-15 | 2020-06-12 | 安徽牧龙山铁皮石斛生物科技开发有限公司 | Extraction process of dendrobium officinale fresh strips for dendrobium wine |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101747446A (en) * | 2008-12-04 | 2010-06-23 | 东北师范大学 | Method for extracting anti-fatigue acidic ginseng polysaccharide |
| CN102614236A (en) * | 2012-04-13 | 2012-08-01 | 吉林农业大学 | Method for processing raw materials of ginseng original drug, food and health care products |
| CN102618616A (en) * | 2012-04-13 | 2012-08-01 | 吉林农业大学 | Method of improving ginsenoside extraction rate by utilizing steam-explosion combined bio-composite enzyme technology |
| CN104398549A (en) * | 2014-11-11 | 2015-03-11 | 长春师范大学 | Method for preparation of total saponins of panax ginseng by high-voltage pulse electric field assisted enzyme hydrolysis |
| CN104758319A (en) * | 2015-03-28 | 2015-07-08 | 珲春绿源参业生物科技有限公司 | Method for preparing traditional Chinese medicine extracting component by multienzyme enzymolysis |
-
2015
- 2015-10-23 CN CN201510718039.1A patent/CN105255962A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101747446A (en) * | 2008-12-04 | 2010-06-23 | 东北师范大学 | Method for extracting anti-fatigue acidic ginseng polysaccharide |
| CN102614236A (en) * | 2012-04-13 | 2012-08-01 | 吉林农业大学 | Method for processing raw materials of ginseng original drug, food and health care products |
| CN102618616A (en) * | 2012-04-13 | 2012-08-01 | 吉林农业大学 | Method of improving ginsenoside extraction rate by utilizing steam-explosion combined bio-composite enzyme technology |
| CN104398549A (en) * | 2014-11-11 | 2015-03-11 | 长春师范大学 | Method for preparation of total saponins of panax ginseng by high-voltage pulse electric field assisted enzyme hydrolysis |
| CN104758319A (en) * | 2015-03-28 | 2015-07-08 | 珲春绿源参业生物科技有限公司 | Method for preparing traditional Chinese medicine extracting component by multienzyme enzymolysis |
Non-Patent Citations (3)
| Title |
|---|
| ZHANG XU 等: "Total fractionation and characterization of the water-soluble polysaccharides isolated from Panax ginseng C. A. Meyer", 《CARBOHYDRATE POLYMERS》 * |
| 宋利华 等: "正交试验优选人参多糖的提取工艺", 《中草药》 * |
| 张祥胜 主编: "《发酵工程实验简明教程》", 31 October 2014, 南京大学出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105582220A (en) * | 2016-02-06 | 2016-05-18 | 济南市第三人民医院 | Preparation method of traditional Chinese medicine for treating diabetic nephropathy |
| CN105768106A (en) * | 2016-03-23 | 2016-07-20 | 北京东方兴企食品工业技术有限公司 | Liver nutrition improving functional food |
| CN108085129A (en) * | 2018-02-09 | 2018-05-29 | 王忠良 | A kind of wood wax oil preparation method |
| CN111269330A (en) * | 2020-04-15 | 2020-06-12 | 安徽牧龙山铁皮石斛生物科技开发有限公司 | Extraction process of dendrobium officinale fresh strips for dendrobium wine |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Su et al. | Inhibitory effects of medicinal mushrooms on α-amylase and α-glucosidase–enzymes related to hyperglycemia | |
| CN103142669B (en) | Enzyme method for extracting ginsenoside and obtained ginsenoside extractive | |
| CN105255579B (en) | Method for extracting peony seed oil | |
| CN102600222B (en) | Method for increasing content of rare saponin in ginseng raw material by explosion of steam | |
| CN104721245A (en) | Method for making ginseng extraction component by multi-enzyme enzymolysis | |
| CN105255962A (en) | Method for extracting blood sugar-reducing panaxan by enzyme method and application of obtained panaxan | |
| CN103193751A (en) | Method for continuously extracting active ingredients from grape seeds | |
| CN104256584A (en) | Moringa oleifera ferment and preparation method thereof | |
| CN102048885A (en) | Extraction process for lychee seed saponin | |
| CN103804503A (en) | Method of extracting polysaccharide of enteromorpha by enzymolysis in steps | |
| CN105085695A (en) | Preparation method for dendrobium candidum extract powder | |
| CN101732359A (en) | Integrated method for poly-generation of Chinese medicinal extracts | |
| CN104840501A (en) | Preparation method for total flavonoids of chrysanthemum | |
| CN103772518A (en) | Extraction method of Chinese yam polysaccharide | |
| CN104398549B (en) | The method that high-pressure pulse electric auxiliary enzyme hydrolysis prepares general ginsenoside | |
| CN104650251A (en) | Extraction method of mythic fungus multi-sugar alcohol | |
| CN106380501A (en) | Method for extracting stigmasterol from coix stems | |
| CN102626459B (en) | Method for compound enzyme-hot water extraction of cortex phellodendri alkaloids | |
| CN101519416B (en) | Method for extracting and separating soluble functional components in hemp in a steam exploded manner | |
| CN102614236B (en) | Method for processing raw materials of ginseng original drug, food and health care products | |
| CN104940280A (en) | Method for extracting total flavones from radix puerariae employing enzyme preparation | |
| CN1416843A (en) | Enzyme prepn method of preparing Chinese medicine | |
| CN103463153A (en) | Method for extracting ginsenoside from American ginseng | |
| CN105384839A (en) | Method for extracting active ingredients from ganoderma lucidum through electrolyzed functional water | |
| CN102091107B (en) | Method for extracting total triterpene of centella asiatica by enzymic process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160120 |