CN101747446A - Method for extracting anti-fatigue acidic ginseng polysaccharide - Google Patents
Method for extracting anti-fatigue acidic ginseng polysaccharide Download PDFInfo
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- CN101747446A CN101747446A CN200810051524A CN200810051524A CN101747446A CN 101747446 A CN101747446 A CN 101747446A CN 200810051524 A CN200810051524 A CN 200810051524A CN 200810051524 A CN200810051524 A CN 200810051524A CN 101747446 A CN101747446 A CN 101747446A
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Abstract
The invention discloses a method for extracting anti-fatigue acidic ginseng polysaccharide. The novel anti-fatigue acidic ginseng polysaccharide is prepared by extracting, purifying and refining ginseng roots which are used as raw materials. The anti-fatigue acidic ginseng polysaccharide can be made into various preparations. The method for extracting the anti-fatigue acidic ginseng polysaccharide retains the activity of the ginseng polysaccharide and improves the purity of the anti-fatigue active components of the ginseng polysaccharide.
Description
The genus field of contracting
The present invention relates to a kind of is raw material with the ginseng, and through extraction, decon, refining, preparation has the extracting method of the acidic ginseng polysaccharide of anti-fatigue effect.
Background technology
Genseng is to be the famous and precious nourishing medicine of people's first-selection since ancient times always.Have and reinforce vital energy, promote the production of body fluid, effect such as calm the nerves.
Growing along with society, people's rhythm of life is accelerated, life stress increase day by day, a lot of people are in sub-health state, and diet does not have rule, and sleep quality descends, and fatigue conditions often occurs.
Fatigue is that the organism physiology process can not continue its function can not keep its predetermined exercise intensity at a specified level or each organ.Fatigue causes necrocytosis or corrupted easily, and cell is the least unit of each organ of human body, so fatigue causes organ function, deterioration easily or directly damage causes organic disease to organ.Because of organism fatigue makes metabolism reduce, the easiest damaging cells of objectionable impurities that can't in time get rid of makes cell aging, death.
Summary of the invention
The object of the present invention is to provide and a kind ofly from ginseng, extract refining method, and propose the new effect of its anti-fatigue active with acidic ginseng polysaccharide of anti-fatigue active.
The present invention can realize by following steps:
1. the extraction of genseng total polysaccharides: carried ginseng 4 hours with the distillation poach under 100 ℃ of conditions.Extract is through 4 layers of filtered through gauze.The residue that filtration obtains repeats to extract twice under the same conditions.Merge three times and extract gained filtrate, centrifugal removal insolubles wherein, 95% ethanol sedimentation that supernatant liquor adds 6 times of volumes spends the night, and centrifugal, collecting precipitation obtains the genseng total polysaccharides after the conventional drying.
2.Sevag method deproteinated: the Sevag reagent that in panaxan's aqueous solution of 10%, adds 1/4 volume, fully concussion is two hours, centrifugal removal egg white layer and chloroform layer, the water layer repetitive operation extremely no longer occurs till the egg white layer, and obtained aqueous solution obtains deproteinated panaxan fraction WGP through ethanol sedimentation and conventional drying.
3. the separation of acidic ginseng sugar: the deproteinated panaxan fraction WGP with 10% is through DEAE-cellulose ion exchange column (Cl
-Type) classification, distilled water wash-out with the twice column volume, remove unconjugated neutral sugar fraction, collect the acid sugar fraction that the 0.5M sodium chloride aqueous solution wash-out by the twice column volume obtains, through distill water dialysis, frost drying obtain the sodium-chlor wash-out in conjunction with fraction WGPA.
The inventive method be worth acidic ginseng polysaccharide, its monose consists of Gal: Glc: Ara: Rha: GalUA: GlcUA=18.0: 18.5: 15.5: 2.5: 44.2: 1.3, range of molecular weight distributions 2.8 * 10
3～1.8 * 10
5Acidic ginseng polysaccharide can be made formulations such as oral preparations, injection, mucosal absorption, Transdermal absorption.
The experiment of panaxan's antifatigue
Male ICR mouse (18-22g), put into separately glass cylinder (10 centimetres of diameters, high 25 centimetres, 10 centimetres of the depth of waters, water temperature 23-25 ℃) in, time remaining 6 minutes writes down its swimming time respectively and maintains static the time, when mouse floats on the water surface with vertical position, and only do small action and stretch out the water surface to keep its head, we are defined as it and maintain static, and the set time is from back four minutes opening entries of swimming, after the first time, forced swimming finished, maintain static the time according to it, we are divided into three groups with mouse, control group, acidic polysaccharose (40 mg/kg) group, neutral polysaccharide (160 mg/kg) group, every group 8, successive administration 15 days, the 15th day, carry out the forced swimming experiment second time, it maintains static the time and is recorded, and administration is after 15 days, heart extracting blood, change/10 minutes separation of serum for 4 ℃ 3000, survey the glucose in its serum, serum lactic dehydrogenase, the content of blood urea nitrogen and total protein.The experimental result (seeing Table 1) of its forced swimming experiment, serum biochemistry parameter (seeing Table 2):
Table 1 panaxan antifatigue forced swimming experimental result
Compare meaning that there was a significant difference with control group: * p＜0.05
Table 2 panaxan antifatigue serum biochemistry experimental result
Compare meaning that there was a significant difference with control group: * p＜0.05
Table 1 is the result show, compare with control group (212.50 ± 12.53), acidic polysaccharose (40mg/kg) can obviously increase mouse swimming time (203.40 ± 19.76), meaning that there was a significant difference (P＜0.05), neutral polysaccharide do not increase mouse swimming time (218.30 ± 19.16) and illustrate that acidic polysaccharose has the activity of antifatigue.
Table 2 is the result show, acidic polysaccharose (40mg/kg) can obviously increase the activity (1071.8 ± 145.08) of mouse serum lactic dehydrogenase, meaning that there was a significant difference (P＜0.05), and neutral polysaccharide does not increase the activity (732.3 ± 131.44) of mouse serum lactic dehydrogenase.By to glucose, the mensuration of blood urea nitrogen and total protein shows that equally acidic polysaccharose (40mg/kg) has the activity of more obvious antifatigue.
Description of drawings
Fig. 1, the DEAE-cellulose linear gradient elution figure of WGP.
Fig. 2, the molecular sieve gel distribution plan of WGPA (sepharose CL-6B).
Fig. 3, the monose composition diagram (HPLC) of WGPA.
Embodiment:
1. take by weighing ginseng 1500 grams, extracted 4 hours for 100 ℃ with 22.5 liters of distilled water, extract is through 4 layers of filtered through gauze.The residue that filtration obtains repeats to extract twice under the same conditions.Merge three times and extract gained filtrate, 4000 rev/mins centrifugal 10 minutes, abandon precipitation, supernatant liquor is concentrated into 700 milliliters for 80 ℃.95% ethanol that adds 4 times of volumes in supernatant liquor, after fully stirring, quiescent setting spends the night.Centrifugal, collecting precipitation, conventional drying (it is centrifugal that 95% ethanol, dehydrated alcohol, ether fully stir the back successively, and the precipitation drying under reduced pressure spends the night).Obtain 152.9 gram genseng raw sugar samples.
2. take by weighing genseng raw sugar 150 gram, be dissolved in 1500 ml waters, adds 375 milliliters of Sevag reagent (propyl carbinol: chloroform=1: 4, v/v), fully shook two hours, after the static layering, 4000 rev/mins centrifugal 10 minutes, removal egg white layer and chloroform layer.The water layer repetitive operation extremely no longer occurs till the egg white layer, and obtained aqueous solution obtains deproteinated panaxan fraction WGP (129 gram) through ethanol sedimentation and conventional drying.
3. WGP (25 gram) is dissolved in the distilled water (250 milliliters), (8.0 * 20cm Cl-) separates with DEAE-cellulose ion exchange column.Use 2 liters of distilled water and 2 liters of 0.5M sodium-chlor wash-outs successively, eluent flow rate is 25 ml/min, per 50 milliliters of elutriants are collected a pipe, detect via the phenol sulfuric acid process, collect suitable fraction, through distill water dialysis after 24 hours, frost drying obtain respectively water elution not in conjunction with fraction WGPN (15.1 gram) and sodium-chlor wash-out in conjunction with fraction WGPA (4.3 restrain).
Claims (5)
1. method of extracting the acidic ginseng polysaccharide with antifatigue effect from ginseng is characterized in that extracting method is:
A. the extraction of genseng total polysaccharides: carried ginseng 4 hours with the distillation poach under 100 ℃ of conditions.Extract is through 4 layers of filtered through gauze.The residue that filtration obtains repeats to extract twice under the same conditions.Merge three times and extract gained filtrate, centrifugal removal insolubles wherein, 95% ethanol sedimentation that supernatant liquor adds 6 times of volumes spends the night, and centrifugal, collecting precipitation obtains the genseng total polysaccharides after the conventional drying.
B.Sevag method deproteinated: the Sevag reagent that in panaxan's aqueous solution of 10%, adds 1/4 volume, fully concussion is two hours, centrifugal removal egg white layer and chloroform layer, the water layer repetitive operation extremely no longer occurs till the egg white layer, and obtained aqueous solution obtains deproteinated panaxan fraction WGP through ethanol sedimentation and conventional drying.
C. the separation of acidic ginseng polysaccharide: the deproteinated panaxan fraction WGP with 10% is through DEAE-cellulose ion exchange column (Cl-type) classification, distilled water wash-out with the twice column volume, remove unconjugated neutral sugar fraction, the acid sugar fraction that collection is obtained by the 0.5M sodium chloride aqueous solution wash-out of twice column volume, through distill water dialysis, frost drying obtain the sodium-chlor wash-out in conjunction with fraction WGPA.
2. according to right 1 described acidic ginseng polysaccharide, it is characterized in that polysaccharide is made up of 6 kinds of monose at least, be respectively Gal, Glc, Ara, Rha, GalUA, GlcUA.
3. according to right 2 described acidic ginseng polysaccharides, it is characterized in that the monose ratio of components is Gal: Glc: Ara: Rha: GalUA: GlcUA=18.0: 18.5: 15.5: 2.5: 44.2: 1.3.
4. according to right 1 described acidic ginseng polysaccharide, it is characterized in that polysaccharide molecular weight is distributed in 2.8 * 10
3With 1.8 * 10
5Between.
5. according to right 1 described acidic ginseng polysaccharide, it is characterized in that polysaccharide can make formulations such as oral preparations, injection, mucosal absorption, Transdermal absorption.
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| CN102584968A (en) * | 2011-12-31 | 2012-07-18 | 上海善力健生物科技有限公司 | Method for extracting and purifying macromolecular active ingredients of plant |
| CN104911233A (en) * | 2014-03-10 | 2015-09-16 | 东北师范大学 | Method for extracting blood glucose lowering ginseng polysaccharides through enzyme method, and applications of method in food and drugs |
| CN105255962A (en) * | 2015-10-23 | 2016-01-20 | 齐齐哈尔医学院 | Method for extracting blood sugar-reducing panaxan by enzyme method and application of obtained panaxan |
| CN106036879A (en) * | 2016-07-04 | 2016-10-26 | 康美（北京）药物研究院有限公司 | Healthcare food capable of relieving physical fatigue and making method thereof |
| CN107098985A (en) * | 2016-02-23 | 2017-08-29 | 石家庄以岭药业股份有限公司 | The preparation method of acidic ginseng polysaccharide is extracted after a kind of alcohol extracting from ginseng in the dregs of a decoction |
| CN107205461A (en) * | 2014-07-29 | 2017-09-26 | 韩国食品研究院 | The ginseng source polysaccharide component handled through ferment and preparation for improving immunity function |
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| CN101250234B (en) * | 2008-04-10 | 2010-12-08 | 北京联合大学生物化学工程学院 | Method for extracting ginseng polysaccharide |
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| CN102584968A (en) * | 2011-12-31 | 2012-07-18 | 上海善力健生物科技有限公司 | Method for extracting and purifying macromolecular active ingredients of plant |
| CN104911233A (en) * | 2014-03-10 | 2015-09-16 | 东北师范大学 | Method for extracting blood glucose lowering ginseng polysaccharides through enzyme method, and applications of method in food and drugs |
| CN104911233B (en) * | 2014-03-10 | 2018-04-13 | 东北师范大学 | The extracting method of panaxan a kind of and its application in hypoglycemic drug and food is prepared |
| CN107205461A (en) * | 2014-07-29 | 2017-09-26 | 韩国食品研究院 | The ginseng source polysaccharide component handled through ferment and preparation for improving immunity function |
| CN105255962A (en) * | 2015-10-23 | 2016-01-20 | 齐齐哈尔医学院 | Method for extracting blood sugar-reducing panaxan by enzyme method and application of obtained panaxan |
| CN107098985A (en) * | 2016-02-23 | 2017-08-29 | 石家庄以岭药业股份有限公司 | The preparation method of acidic ginseng polysaccharide is extracted after a kind of alcohol extracting from ginseng in the dregs of a decoction |
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| CN110140954A (en) * | 2019-06-14 | 2019-08-20 | 卓雍皓 | A kind of Qamgur meal replacement powder and preparation method thereof |
| CN112409502A (en) * | 2020-07-01 | 2021-02-26 | 吉林化工学院 | Method for separating and characterizing ginseng polysaccharide with immunoregulation and anti-tumor activity |
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