CN1957958A - Method for preparing extractive of ginkgo leaves - Google Patents
Method for preparing extractive of ginkgo leaves Download PDFInfo
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- CN1957958A CN1957958A CN 200610032071 CN200610032071A CN1957958A CN 1957958 A CN1957958 A CN 1957958A CN 200610032071 CN200610032071 CN 200610032071 CN 200610032071 A CN200610032071 A CN 200610032071A CN 1957958 A CN1957958 A CN 1957958A
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Abstract
A process for preparing the extract of gingko leaf includes such steps as mixing fresh gingko leaves with water, beating, extruding to obtain liquid and dregs, and extracting from the obtained liquid. Its advantage is no use of organic solvent.
Description
Technical field
The present invention relates to the process that a kind of plant extract refers in particular to the preparation Folium Ginkgo extract.
Background technology
Folium Ginkgo (Trifolium pratense L.) is the leaf of Ginkgoaceae Ginkgo plant Ginkgo biloba (Ginkgo biloba L.).Folium Ginkgo extract contains effective ingredient such as flavonoid, terpene lactones, Polyphenols, organic acid, polyisoamylene alcohol various trace elements, have antioxidation, the effect of antiplatelet activity factor, blood fat reducing, blood glucose effect, improve the heart, the cerebral blood circulation effect, to the pharmacology clinical effects such as protective effect of central nervous system, digestive system, respiratory system.With Folium Ginkgo and Folium Ginkgo extract is medicine, health product, cosmetics and the fashionable whole world of other Related products that raw material is made, the global marketing volume prostatitis that Folium Ginkgo extract is sure to occupy for many years.Semen Ginkgo is one of special product plant of China, also is one of China's large outlet botanical drug substance kind, and aboundresources accounts for world's total amount more than 70%, has vast market prospect.
The extracting method of Folium Ginkgo extract mainly contains both at home and abroad: water or organic solvent method: water or organic solvent extraction, and the purified method of binding resin is as CN116532A; The supercritical extraction method is as CN172669; The high speed adverse current chromatogram extraction method; Methods such as enzyme process.
Extract Folium Ginkgo with water extraction, relevant patent or report are also arranged in recent years.With the Folium Ginkgo extract (EGB) of water extraction explained hereafter, carry now maximum be the extraction process by water that in water, adds HB one 96 additives, to add the water as solvent of HB one 96 special additives, the abundant lixiviate of active ingredient in the Folium Ginkgo is come out.
There is certain deficiency in these methods: as organic solvent method production cost height, environmental effect is big; The purified method of binding resin produces still high, and the product water solublity is bad; Supercritical extraction method and high speed adverse current chromatogram extraction method etc. need the more fund of input ratio, the production cost height on equipment when being used for commercial production.
With fresh Folium Ginkgo is that raw material extracts, and relevant patent or report are also arranged in recent years.
CN1436784A (producing extractant and the extraction and the analytical method of high-activity ginkgo leaf extract), this invention adopts sulphite or bisulfites 0.001-0.5 water to be supplemented to 100, pH value is the extractant of 2.0-5.5, after this extractant extraction, extracting liquid filtering, filtrate are crossed the resin absorption post, use the lower alcohol eluting, eluent concentrates, and drying obtains Folium Ginkgo extract.In this patent, relevant raw material can be the bright leaf of Semen Ginkgo, or through exsiccant Folium Ginkgo, described leaf does not need through pulverizing.Sulfate or bisulfite salt additives have been added in this invention in water, raw material is not made relevant treatment but directly carry out water and carry out lixiviate, thereby influence follow-up yield and extraction ratio easily, and its corresponding production cost is also higher.
CN1481793 (a kind of Folium Ginkgo extract and contain its injection), this invention relates to a kind of Folium Ginkgo extract, this extract prepares by the following method: bright Folium Ginkgo cleans making beating, stiff serosity ethanol extraction, alcohol extract adjusting PH with base 8-10 filters, and filtrate is transferred pH3-8 with acid, preferred 5-6, filter, filtrate recycling ethanol obtains dark brown dope, hot water extraction, the cold preservation of water extract is filtered, macroporous resin column desalination on the filtrate, gradient ethanol elution, reclaim ethanol, the dense ginkgo biloba succi general flavone content of optional detection.This invention also relates to YINXINGYE ZHUSHEYE, and its preparation comprises further makes injection with the said extracted thing.What this invention was mainly adopted is alcohol extraction, and processing step is also more also comparatively complicated, is unfavorable for the actual production operation, and production cost also can correspondingly increase.
Summary of the invention
The preparation method of the Folium Ginkgo extract that a kind of cost is low in order to provide for purpose of the present invention, technology can improve extraction ratio relatively simply, again, impurity in products is few and low in the pollution of the environment.
The preparation of extract realizes by following manner among the present invention:
It is raw material that the present invention adopts fresh Folium Ginkgo, and fresh Folium Ginkgo is added the water making beating, and squeezing gets pressed liquor and press residue, and the pressed liquor extraction separation is made Folium Ginkgo extract.
The pressed liquor that obtains by squeezing among the present invention can obtain most of bioactive ingredients in the raw material, directly uses the pressed liquor extraction separation, just can obtain having the Folium Ginkgo extract of most of active component.
Described pressed liquor is extraction separation in the following way: with pressed liquor, filter, filtrate is crossed macroporous adsorptive resins, water and ethanol elution, collect alcohol eluen, concentrate, after the drying pressed liquor crude extract with the ethanol extraction more than 90% make with extra care Folium Ginkgo extract.
Because also have the sub-fraction active component in the press residue, at the press residue part, the inventor uses water extraction, again press residue is carried out extraction separation, can further fully utilize fresh Folium Ginkgo raw material like this, has also improved extraction ratio and yield simultaneously.
Described press residue is extraction separation in the following way: with the press residue hot water extraction, filter, filtrate is crossed macroporous adsorptive resins, water and ethanol elution, collect alcohol eluen, concentrate, after the drying press residue crude extract with the ethanol extraction more than 90% make with extra care Folium Ginkgo extract.
Or with the crude extract of the crude extract of the press residue that obtains after the drying and pressed liquor merge with 90% above ethanol extraction make with extra care Folium Ginkgo extract.
Described to add water making beating be to be 2-10 with weight ratio: 1 water and bright Folium Ginkgo mixing homogenate.
What the raw material of described Folium Ginkgo extract adopted is fresh state, and the Semen Ginkgo greenery of plucking in 4-10 month, best collecting season are the 6-7 months.The best age of tree of gathering is 3-5.
The inventor discovers that in the present invention the extract by the Folium Ginkgo of following explained hereafter is optimum process route.At first add water making beating with fresh Folium Ginkgo, obtain pressed liquor and press residue after the squeezing, pressed liquor is crossed macroporous adsorptive resins, and water and ethanol elution are collected alcohol eluen, concentrate, dry back with the ethanol extraction more than 90% make with extra care Folium Ginkgo extract.The content of the resulting extract of this a part of pressed liquor (Folium Ginkgo total flavones alcohol glycosides is greater than 30%, and total terpene lactones is greater than 10%).The press residue hot water extraction, filter, filtrate is crossed macroporous adsorptive resins, water and ethanol elution, collect alcohol eluen, concentrate, the dry after ethanol extraction 90% or more refining Folium Ginkgo extract, this part is by the content (Folium Ginkgo total flavones alcohol glycosides is greater than 24%, and always terpene lactones is greater than 6%) of the extract of press residue gained.This process is the easiest, cost is low, impurity level is few, and environmental pollution is little.
Folium Ginkgo extract active substance flavonol glycosides by gained of the present invention is not less than 24%, total terpene lactones is not less than 6%, belong to " friendly process ", dissolvent residual is few, the biological activity height can be used as Folium Ginkgo capsule, tablet, soft gelatin capsule, drop pill etc., especially the quality raw materials of YINXINGYE ZHUSHEYE, healthy beverage, cosmetics etc., carry out deep processing, make corresponding product.
In disclosure of the Invention CN1481793, adopt fresh Folium Ginkgo making beating, serosity is then directly used alcohol extraction, different with pressed liquor among the present invention with press residue difference processing method, this processing respectively makes that the present invention's process thereafter is more targeted, help improving extraction ratio, reduce impurity etc., and press residue is also used water extraction among the present invention, is not to use alcohol extraction.It is the yield largest portion that the present invention makes extract by pressed liquor, this separate processes is most of active component to be extracted according to pressed liquor, press residue part water enters cell interior once more and extracts, when concentrating on crucial points, fully utilize fresh Folium Ginkgo raw material, also improved extraction ratio and yield at the same time.
The present invention takes water extraction, squeeze processing earlier, consumption of organic solvent significantly reduces, owing to be that bioactive ingredients is that homogeneous state exists in the fresh Folium Ginkgo body, the Cell sap system stability, and its cell wall is relatively more fragile, at this moment carries out the homogenate squeezing, cell wall is destroyed, and active component is come out by rapid extraction.And how prior art carries out organic solvent extraction with cured leaf, and the keratinization of the cell wall of cured leaf, shipwreck is to enter cell interior, and the Cell sap system environments changes, and can only use organic solvent extraction.The principle of this extraction is that organic solvent is owing to diffusion, osmosis penetrate in the cell by cell wall gradually, dissolved active substance, and cause concentration difference inside and outside the cell, so intracellular concentrated solution is constantly to external diffusion, organic solvent constantly enters again in the cell, so repeatedly comes and goes, when solution concentration reaches dynamic equilibrium inside and outside cell, this saturated solution is leached, continue repeatedly to add novel solvent, just as much as possible with the active component stripping.And such extraction has not only improved production cost, and organic solvent has also increased isolating difficulty in leaching process.The present invention surpasses 3% by the yield of the Folium Ginkgo extract of the pressed liquor acquisition of squeezing back gained, the active component content flavonol glycosides is greater than 30%, total terpene lactones is greater than 10%, suffice to show that its advantage, cross post with squeezeding juice, the alcohol eluen concentrate drying, the back is refining with 90% above ethanol, this be now all patents with report in technology not all from relating to.
And the press residue after the squeezing, though active component content, is used water extraction less than the pressed liquor height, water is easy to enter cell interior, and the extract active component content flavonol glycosides of acquisition is greater than 24%, and total terpene lactones is greater than 6%.
Adopt technology of the present invention, consumption of organic solvent significantly reduces, and obtain having higher yield and high-load finished product, especially be embodied on the prepared extract of optimization process by the pressed liquor part, show that the present invention is low-cost, high yield, high-load environment-protective process method, for becoming medicine, health product, cosmetics etc. that the basic technology support is provided, help developing smoothly Related product with the Folium Ginkgo extract deep processing.
The inventor is by to the analysis on Content of the different processing mode of Folium Ginkgo to flavonol glycosides in the Folium Ginkgo and terpene lactones, the flavonol glycosides of the dry Folium Ginkgo (dry or dry) that discovery is commonly used is compared with the aquatic foods product with terpene lactones content, gap is quite obvious, and flavonol glycosides and terpene lactones content are the highest in the bright Folium Ginkgo.How existing Folium Ginkgo extraction process is that raw material extracts with the Folium Ginkgo dry product, and according to do, the contrast of bright composition learns that active constituent content is higher than cured leaf in the bright leaf, and content is gradually low with the growth of period of storage.Therefore adopting the Folium Ginkgo of fresh state among the present invention is raw material.Concrete data see the following form:
Table 1: the flavonol glycosides of the Folium Ginkgo of different crude drug processing modes and the content of terpene lactones
| Oven dry | 1.267 | 0.328 | |
| Gansu | Bright product | 1.037 | 0.750 |
| Dry | 0.926 | 0.739 | |
| Oven dry | 0.887 | 0.514 | |
| The Liuyang | Bright product | 0.624 | 0.682 |
| Dry | 0.602 | 0.581 | |
| Oven dry | 0.568 | 0.475 |
The inventor is by the investigation to whole growth cycle of fresh medical material, finds effective ingredient Biomass the highest period to be raw material with this stage Folium Ginkgo, can reduce industrial production cost greatly, improves the product yield.Found that the age of tree is 3-5, the fresh medical material of the Folium Ginkgo of the 6-7 month is the optimum feed stock condition mode.Concrete data see the following form, table 2, and June, 12-16 collected the fresh Folium Ginkgo of the different age of trees between day, carried out the resulting tables of data of assay, and table 3 is to the assay of the fresh Folium Ginkgo of different time of 4 years living age of trees.
Table 2 12-16 in June collects the flavonol glycosides of fresh Folium Ginkgo of the different age of trees and the content of terpene lactones between day
The inventor is not that Folium Ginkgo below 3 years deals with to the age of tree, and on the one hand because the Semen Ginkgo of a year and life in 2 years, plant is little, and leaves is few, that is to say that Folium Ginkgo biology total amount is few, on the other hand, is unfavorable for the growth of Semen Ginkgo in the harvesting in this stage.Therefore, the inventor only researchs and analyses the Semen Ginkgo more than 3 years the age of tree.
In to the content analysis of the age of tree to the flavonol glycosides of Folium Ginkgo and terpene lactones, find that the content of Folium Ginkgo flavone alcohol glycosides that 3-5 gives birth to and terpene lactones is the highest, be the best age of tree of gathering.But consider manufacturer's comprehensive profit of Folium Ginkgo, in conjunction with the content analysis to Folium Ginkgo, the Folium Ginkgo that 3-10 gives birth to is all available, and just, along with the growth of the age of tree, the content of flavonol glycosides and terpene lactones reduces in the raw material, can influence extraction ratio and yield.
The flavonol glycosides of the fresh Folium Ginkgo of the different time of 4 years living age of trees of table 3 and the content of terpene lactones
Folium Ginkgo is in autumn Mo meeting flavescence, be generally the greenery state time in the 4-10 month, April to late May is positioned at the leaf stretching period of Folium Ginkgo, the Folium Ginkgo Biomass is few, and the Folium Ginkgo collecting period can have a negative impact to the growth of Semen Ginkgo too early, so among the present invention, the Semen Ginkgo greenery of raw material for plucking the 4-10 month, and the 6-7 month is the raw material optimal harvest time.
The inventor consider Folium Ginkgo in the secondary metabolism process effective ingredient such as its flavonol glycosides and terpene lactones in the feature of the high level of trophophase accumulation, in conjunction with the process of optimizing, improved the extraction ratio of effective constituents of raw material, help the high-purity extract preparation in later stage and improve the product yield, reduce cost; With this extract is the raw material deep processing, and exploitation medicine, cosmetics, health product etc. have more excellent quality.
Among the present invention fresh Folium Ginkgo is added the water making beating, get pressed liquor and press residue after the squeezing,, make Folium Ginkgo extract pressed liquor and press residue difference extraction separation.Be prepared through the pressed liquor of squeezing gained and also available other existing method of extraction separation method of press residue, as organic solvent method: for example press residue is adopted the ethanol lifting manipulation, with the press residue alcohol reflux, extracting solution concentrates, and hot water extraction filters, filtrate is crossed post, water and ethanol elution are collected alcohol eluen, concentrated, the dry Folium Ginkgo extract that gets; As the supercritical extraction method, for example adopt supercritical carbon dioxide extraction method, enzyme process, high speed adverse current chromatogram extraction method etc.
The specific embodiment
Following examples are intended to illustrate the present invention rather than limitation of the invention further.
Embodiment 1
Choose fresh state, not have the age of tree addle, to pluck July be the bright Folium Ginkgo 1kg in 3 years, add 2kg water and be broken into homogenate, squeezing.Pressed liquor is crossed the D101 macroporous resin column after using the filter paper sucking filtration, elder generation's water eluting, 70% ethanol elution is used in the back, collects 70% ethanol elution, concentrates, dry, obtain the pressed liquor crude extract, with pressed liquor crude extract 90% alcohol reflux, extracting solution through alcohol reclaim, extracting solution concentrates, after the drying the 8.2g extract, recording content is flavonol glycosides 34.4%, terpene lactones 14.1%.
Raw material is given money as a gift and counted total recovery is 2.79%.
Embodiment 2
Choose fresh state, not have the age of tree addle, to pluck July be the bright Folium Ginkgo 1kg in 4 years, add 2kg water and be broken into homogenate, squeezing.Pressed liquor is crossed the D101 macroporous resin column after using the filter paper sucking filtration, elder generation's water eluting, 70% ethanol elution is used in the back, collects 70% ethanol elution, concentrates, dry, obtain the pressed liquor crude extract, with pressed liquor crude extract 90% alcohol reflux, extracting solution through alcohol reclaim, extracting solution concentrates, after the drying the 6.0g extract, recording content is flavonol glycosides 32.6%, terpene lactones 14.1%.
Press residue filters with 5 times of calorimetric water extraction 2 hours, and filtrate is crossed the D101 macroporous resin column, elder generation's water eluting, 70% ethanol elution is used in the back, collects 70% ethanol elution, concentrates, dry, obtain the press residue crude extract, with press residue crude extract 90% alcohol reflux, extracting solution through alcohol reclaim, extracting solution concentrates, after the drying the 3.4g extract, recording content is flavonol glycosides 26.8%, terpene lactones 8.3%.
Raw material is given money as a gift and counted total recovery is 3.10%.
Embodiment 3
Choose fresh state, not have the age of tree addle, to pluck July be the bright Folium Ginkgo 1kg in 4 years, adds 2kg water and be broken into homogenate, the water that adds 1kg again stirred 1 hour, squeezing.Pressed liquor is crossed the D101 macroporous resin column after using the filter paper sucking filtration, first water eluting, and 70% ethanol elution is used in the back, collects 70% ethanol elution, concentrates, and drying obtains the pressed liquor crude extract.
Press residue filters with 5 times of calorimetric water extraction 2 hours, and filtrate is crossed the D101 macroporous resin column, first water eluting, and 70% ethanol elution use in the back, collects 70% ethanol elution, and concentrated, drying obtains the press residue crude extract.
Pressed liquor crude extract and press residue crude extract are merged, use 90% alcohol reflux, extracting solution through alcohol reclaim, extracting solution concentrates, must the 9.8g extract after the drying, recording content is flavonol glycosides 28.9%, terpene lactones 11.2%.
Raw material is given money as a gift and counted total recovery is 3.23%.
Embodiment 4
Choose fresh state, not have the age of tree addle, to pluck June be the bright Folium Ginkgo 1kg in 3 years, adds 4kg water and be broken into homogenate, the water that adds 4kg again stirred 1 hour, squeezing.Pressed liquor is crossed the D101 macroporous resin column after using the filter paper sucking filtration, first water eluting, and 70% ethanol elution is used in the back, collects 70% ethanol elution, concentrates, and drying obtains the pressed liquor crude extract.With pressed liquor crude extract 90% alcohol reflux, extracting solution through alcohol reclaim, extracting solution concentrates, after the drying the 8.1g extract, recording content is flavonol glycosides 34.9%, terpene lactones 14.4%.
Press residue filters with 5 times of calorimetric water extraction 2 hours, and filtrate is crossed the D101 macroporous resin column, elder generation's water eluting, 70% ethanol elution is used in the back, collects 70% ethanol elution, concentrates, dry, obtain the press residue crude extract, with press residue crude extract 90% alcohol reflux, extracting solution through alcohol reclaim, extracting solution concentrates, after the drying the 3.5g extract, recording content is flavonol glycosides 27.2%, terpene lactones 7.8%.
Raw material give money as a gift the meter total recovery be: 3.89%
Embodiment 5
Choose fresh state, not have the age of tree addle, to pluck June be the bright Folium Ginkgo 1kg in 3 years, add 2kg water and be broken into homogenate, squeezing.Pressed liquor is crossed the D101 macroporous resin column after using the filter paper sucking filtration, first water eluting, and 70% ethanol elution is used in the back, collects 70% ethanol elution, concentrates, and drying obtains the pressed liquor crude extract,
Press residue filters with 5 times of calorimetric water extraction 2 hours, and filtrate is crossed the D101 macroporous resin column, first water eluting, and 70% ethanol elution use in the back, collects 70% ethanol elution, and concentrated, drying obtains the press residue crude extract.
Pressed liquor crude extract and press residue crude extract are merged, use 90% alcohol reflux, extracting solution through alcohol reclaim, extracting solution concentrates, must the 12g extract after the drying, recording content is flavonol glycosides 32.3%, terpene lactones 12.1%.
Raw material give money as a gift the meter total recovery be: 4.02%
Embodiment 6
Choose fresh state, not have the age of tree addle, to pluck June be the bright Folium Ginkgo 1kg in 7 years, adds 4kg water and be broken into homogenate, the water that adds 6kg again stirred 1 hour, squeezing.Pressed liquor is crossed the D101 macroporous resin column after using the filter paper sucking filtration, elder generation's water eluting, 70% ethanol elution is used in the back, collects 70% ethanol elution, concentrates, dry, obtain the pressed liquor crude extract, with pressed liquor crude extract 90% alcohol reflux, extracting solution through alcohol reclaim, extracting solution concentrates, after the drying the 5.4g extract, recording content is flavonol glycosides 26.9%, terpene lactones 13.2%.
Press residue filters with 5 times of calorimetric water extraction 2 hours, and filtrate is crossed the D101 macroporous resin column, elder generation's water eluting, 70% ethanol elution is used in the back, collects 70% ethanol elution, concentrates, dry, obtain the press residue crude extract, with press residue crude extract 90% alcohol reflux, extracting solution through alcohol reclaim, extracting solution concentrates, after the drying the 3.6g extract, recording content is flavonol glycosides 26.5%, terpene lactones 7.3%.
Raw material is given money as a gift and counted total recovery is 3.06%.
Embodiment 7
Choose fresh state, not have the age of tree addle, to pluck June be the bright Folium Ginkgo 1kg in 7 years, adds 5kg water and be broken into homogenate, the water that adds 2kg again stirred 1 hour, squeezing.Pressed liquor is crossed the D101 macroporous resin column after using the filter paper sucking filtration, first water eluting, and 70% ethanol elution is used in the back, collects 70% ethanol elution, concentrates, and drying obtains the pressed liquor crude extract.
Press residue filters with 5 times of calorimetric water extraction 2 hours, and filtrate is crossed the D101 macroporous resin column, first water eluting, and 70% ethanol elution use in the back, collects 70% ethanol elution, and concentrated, drying obtains the press residue crude extract.
Pressed liquor crude extract and press residue crude extract are merged, use 90% alcohol reflux, extracting solution through alcohol reclaim, extracting solution concentrates, must the 9.3g extract after the drying, recording content is flavonol glycosides 26.1%, terpene lactones 10.6%.
Raw material is given money as a gift and counted total recovery is 3.16%.
Embodiment 8
Choose fresh state, nothing addles, the age of tree of plucking June is the bright Folium Ginkgo 600kg in 3 years, add 2000kg water, broken pulping, add water 1000kg again, stirred 1 hour, diluent is crossed the pressing filtering liquid that filter press is made clear, and filtrate is crossed the D101 macroporous adsorptive resins, behind the water eluting, use 70% ethanol elution, collect ethanol elution, be concentrated into the 10-15 Baume, vacuum drying gets the pressing filtering liquid crude extract, the pressing filtering liquid crude extract filters with 90% alcohol reflux 1 hour, dry Folium Ginkgo extract 4.02kg, recording content is flavonol glycosides 34.6%, terpene lactones 14.7%.
Filter-press residues extracted 2 hours with 5 times of water gagings, filter, filtrate is crossed the D101 macroporous adsorptive resins, first water eluting, back 70% ethanol elution, collect ethanol elution, concentrate drying gets the filter-press residues crude extract, and the filter-press residues crude extract was with 90% alcohol reflux 1 hour, filter, the dry Folium Ginkgo extract 1.9kg that gets, recording content is flavonol glycosides 25.7%, terpene lactones 7.6%.
Raw material is given money as a gift and counted total recovery is 3.46%.
Embodiment 9
Choose fresh state, not have the age of tree addle, to pluck June be the bright Folium Ginkgo 600kg in 3 years, add 2400kg water, broken pulping adds water 3000kg again, stirred 1 hour, diluent is crossed the pressing filtering liquid that filter press is made clear, and filtrate is crossed the D101 macroporous adsorptive resins, behind the water eluting, use 70% ethanol elution, collect ethanol elution, be concentrated into the 10-15 Baume, vacuum drying gets the pressing filtering liquid crude extract.
Filter-press residues extracted 2 hours with 5 times of water gagings, filtered, and filtrate is crossed the D101 macroporous adsorptive resins, first water eluting, and back 70% ethanol elution is collected ethanol elution, and concentrate drying gets the filter-press residues crude extract.
Pressing filtering liquid crude extract and filter-press residues crude extract are merged,, filter with 90% alcohol reflux 1 hour, dry Folium Ginkgo extract 7.03kg, recording content is flavonol glycosides 30.6%, terpene lactones 12.2%.
Raw material is given money as a gift and counted total recovery is 3.71%.
Claims (8)
1, the preparation method of Folium Ginkgo extract adds the water making beating with fresh Folium Ginkgo, and squeezing gets pressed liquor and press residue, and pressed liquor is carried out extraction separation, makes Folium Ginkgo extract.
2, the preparation method of Folium Ginkgo extract according to claim 1, described pressed liquor is extraction separation in the following way: pressed liquor is filtered, filtrate is crossed macroporous adsorptive resins, elder generation's water, use ethanol elution then, collect alcohol eluen, concentrate, after the drying pressed liquor crude extract with 90% above ethanol extraction make with extra care Folium Ginkgo extract.
3, the preparation method of Folium Ginkgo extract according to claim 1 is carried out extraction separation with press residue again, makes Folium Ginkgo extract.
4, the preparation method of Folium Ginkgo extract according to claim 3, the press residue of gained is extraction separation in the following way: use hot water extraction, filter, filtrate is crossed macroporous adsorptive resins, elder generation's water, use ethanol elution then, collect alcohol eluen, concentrate, after the drying press residue crude extract with 90% above ethanol extraction make with extra care Folium Ginkgo extract.
5, according to the preparation method of claim 2 or 4 described Folium Ginkgo extract, with the crude extract of the crude extract of press residue and pressed liquor merge with 90% above ethanol extraction make with extra care Folium Ginkgo extract.
6, it is to be 2-10 with weight ratio that the preparation method of Folium Ginkgo extract according to claim 1, described fresh Folium Ginkgo add water making beating: 1 water mixes making beating with bright Folium Ginkgo.
7, the preparation method of Folium Ginkgo extract according to claim 1, described fresh Folium Ginkgo are the Semen Ginkgo greenery of plucking the 4-10 month.
8, the preparation method of Folium Ginkgo extract according to claim 7, described fresh Folium Ginkgo are to be the 3-5 ginkgo by the age of tree, in the Semen Ginkgo greenery of plucking the 6-7 month.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101884408A (en) * | 2010-06-24 | 2010-11-17 | 重庆市武隆县海景中药研究所 | Ginkgo leaf leaching liquor and preparation method thereof |
| CN101904891A (en) * | 2010-07-27 | 2010-12-08 | 成都天宇联盟医药科技有限责任公司 | Ginkgo leaf pills and preparation method thereof |
| CN102727540A (en) * | 2012-06-25 | 2012-10-17 | 陆建益 | Method for preparing red bayberry leaf extract |
-
2006
- 2006-08-09 CN CNB2006100320715A patent/CN100556416C/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101884408A (en) * | 2010-06-24 | 2010-11-17 | 重庆市武隆县海景中药研究所 | Ginkgo leaf leaching liquor and preparation method thereof |
| CN101904891A (en) * | 2010-07-27 | 2010-12-08 | 成都天宇联盟医药科技有限责任公司 | Ginkgo leaf pills and preparation method thereof |
| CN102727540A (en) * | 2012-06-25 | 2012-10-17 | 陆建益 | Method for preparing red bayberry leaf extract |
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| CN100556416C (en) | 2009-11-04 |
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