CN105254606A - Method for extracting procyanidins from purple corn - Google Patents
Method for extracting procyanidins from purple corn Download PDFInfo
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- CN105254606A CN105254606A CN201510667778.2A CN201510667778A CN105254606A CN 105254606 A CN105254606 A CN 105254606A CN 201510667778 A CN201510667778 A CN 201510667778A CN 105254606 A CN105254606 A CN 105254606A
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Abstract
The invention discloses a method for extracting procyanidins from purple corn. The purple corn is taken as a main raw material, and enzymic preparations are adopted and combined with a solvent for extraction of the procyanidins. The preparation method comprises steps as follows: material preparation, preparation of a buffer solution, preparation of an enzyme liquid, feeding, enzymolysis, extraction and obtaining of a finished product. The procyanidins prepared with the method has good safety and better quality, few enzymic preparations are used, the extraction rate of the procyanidins is 30%-40% higher than that with an ethanol extraction method, and the method is simple in process, convenient to operate, high in efficiency and suitable for industrial production.
Description
Technical field
The invention belongs to natural product processing scientific domain, particularly a kind of method utilizing purple corn to extract pycnogenols.
Background technology
Be otherwise known as pycnogenols (Procyanidins is called for short OPC) oligomeric flavanone, is a kind of new and effective antioxidant, is the most potent found so far free-radical scavengers, has very strong activity in vivo.Experiment proves, the Green Tea Extract oxidation capacity of OPC is 50 times of vitamin-E, ascorbic 20 times, and absorbs rapidly completely, and within oral 20 minutes, can reach the highest haemoconcentration, metabolic half life is for 7 hours.
Pycnogenols is kind the abundantest in nature phenolic compound, and pycnogenols produces coloured anthocyanidin because there is typical oxidation DeR under acid medium and gains the name.In all kinds of pycnogenols, dimer is the class pycnogenols that is the widest, most study that distributes.Report both at home and abroad a lot about the pharmacotoxicological effect of pycnogenols, the functions such as such as scavenging free radicals, strong anti-oxidation, antitumor and arteriosclerosis; Pycnogenols has also been used for the complication after preventing Operation of Cataract in Diabetics; In addition pycnogenols also serves vital role in plant defense predator and microbial mechanism.So act on widely in view of pycnogenols has, its extracting method also receives the very big concern of domestic and international researchist.
Purple corn (purplecorn, formal name used at school ZeamaysL), seed is yellow, and other parts such as plant are purple, and in purple plant, pigment content is high, and extracted amount is large, and the pigment extracting gained is bright in colour.In China, black waxy corn and purple corn are a kind of crops that each department are planted in a large number, and most of corn cob is abandoned in vain or burns in the course of processing, not only wastes Biological resources, and contaminate environment.Purple corn plant is most as silage, and added value is lower.Therefore, utilize black waxy corn and purple corn to extract natural pigment and not only can avoid the wasting of resources, protection of the environment, meet the need of market, also can promote added value and the comprehensive utilization ratio of corn, simultaneously for new resources are found in the preparation of pycnogenols.
Summary of the invention
The object of the invention is the deficiency overcoming existing extracting method, propose a kind of method utilizing purple corn to extract pycnogenols.The pycnogenols product that this method obtains can be used for the industry such as medicines and health protection and makeup.
A kind of method utilizing purple corn to extract pycnogenols of the present invention, raw material is purple corn, and reagent has polygalacturonase (30000IU), cellulase (15000IU), 95% ethanol (food grade), citric acid (food grade), Sodium phosphate dibasic (food grade).
A kind of method utilizing purple corn to extract pycnogenols of the present invention, is made up of following steps:
(1) get the raw materials ready: purple corn is carried out constant temperature drying under reduced pressure, pulverize after weighing, 40 orders sieve, and obtain raw material dry powder for subsequent use;
(2) buffered soln is prepared: mixed with the volume ratio of 0.2mol/L disodium phosphate soln by 1:1.5 ~ 2 by 0.1mol/L citric acid solution, then thin up 2 times, be mixed with buffered soln:
(3) prepare enzyme liquid: mixing buffered soln, polygalacturonase and cellulase, and fully stirring makes it dissolve completely, thus form uniform enzyme liquid; The consumption of buffered soln is in raw material dry powder weight (kg): the ratio calculating polygalacturonase of volume of buffer solution (L)=1:15 ~ 30 and the consumption of cellulase are that the buffered soln of every L adds 0.1 ~ 0:3g polygalacturonase and 0.1 ~ 0.3g cellulase;
(4) feed intake: in raw material dry powder weight (kg): the ratio of volume of buffer solution (L)=1:15 ~ 30, raw material dry powder is added in enzyme liquid;
(5) enzymolysis: after raw material dry powder and enzyme liquid are stirred, lucifuge insulation in the thermostat water bath of 50 ~ 55 DEG C, every 30min stirs once, enzymolysis 3-4h, generates the mixture of enzymolysis solution and residue;
(6) extract: 75 ~ 95% ethanol are added in above-mentioned enzymolysis solution and residue mixture by the method adding 75 ~ 95% ethanol of 15 ~ 30L by every kg raw material dry powder, under 60 ~ 65 DEG C of conditions, extract 2 ~ 3h, after filtration, obtain extracting solution;
(7) finished product: putting extracting solution in the baking oven of 60 ~ 70 DEG C dries to constant weight, and obtains pycnogenols.
A kind of method utilizing purple corn to extract pycnogenols of the present invention, take purple corn as main raw material, adopt zymin in conjunction with solvent extraction pycnogenols, its advantage is that former blue and white plain Product Safety is good, quality is good, and former blue and white plain extraction yield comparatively alcohol extracting is followed the example of high by 30 ~ 40%, and the consumption of zymin is few, and technique is simple, easy to operate, efficiency is high, is applicable to suitability for industrialized production.The pectin substance using polygalacturonase and cellulase can destroy cell wall structure based on Mierocrystalline cellulose in plant material and iuntercellular thereof to be connected, makes extraction resistance to mass transfer reduce, makes the former blue and white plain composition of content be easy to stripping.Ethanolic soln is as the Extraction solvent of the former blue and white element of alcohol dissolubility.Citric acid phosphoric acid buffered soln plays the effect of regulator solution pH value.By the buffered soln that method of the present invention is prepared, its pH=6 after testing, can ensure the best effect of zymin effect.
Specific embodiment: in order to fully disclose a kind of method utilizing purple corn to extract pycnogenols of the present invention, be illustrated below in conjunction with embodiment.
Embodiment: a kind of method utilizing purple corn to extract pycnogenols
The purple corn getting collection period is raw material, and extracting method comprises the following steps:
L () puts fresh purple corn dries in 65 DEG C of baking ovens, pulverizes, within 40th, sieve, obtain raw material dry powder for subsequent use with high-speed tissue mashing machine.
(2) get 0.1mol/L citric acid solution 160ml, 0.2mol/L disodium phosphate soln 320ml, mix and dilute and be settled to 1000ml, be mixed with buffered soln.
(3) get the buffered soln of 250ml, add 0.05g polygalacturonase and 0.075g cellulase, stir and make it dissolve completely, form uniform enzyme liquid.
(4) in above-mentioned enzyme liquid, add the raw material dry powder of 10g.
(5) lucifuge insulation in the thermostat water bath of 50 DEG C after stirring, every 30min stirs once, enzymolysis 3h, generates the mixture of enzymolysis solution and residue.
(6) in the mixture of enzymolysis solution and residue, add 250m195% ethanol, under 60 DEG C of conditions, extract 2h, after filtration extracting solution.
(7) putting extracting solution in the baking oven of 65 DEG C dries to constant weight, and obtains pycnogenols.
Claims (2)
1. utilize purple corn to extract a method for pycnogenols, it is characterized in that being made up of following steps:
(1) get the raw materials ready: purple corn is carried out constant temperature drying under reduced pressure, pulverize after weighing, 40 orders sieve, and obtain raw material dry powder for subsequent use;
(2) buffered soln is prepared: mixed with the volume ratio of 0.2mol/L disodium phosphate soln by 1:1.5 ~ 2 by 0.1mol/L citric acid solution, then thin up 2 times, be mixed with buffered soln;
(3) prepare enzyme liquid: mixing buffered soln, polygalacturonase and cellulase, and fully stirring makes it dissolve completely, thus form uniform enzyme liquid; The consumption of buffered soln is in raw material dry powder weight (kg): the ratio calculating polygalacturonase of volume of buffer solution (L)=1:15 ~ 30 and the consumption of cellulase are that the buffered soln of every L adds 0.1 ~ 0:3g polygalacturonase and 0.1 ~ 0.3g cellulase;
(4) feed intake: in raw material dry powder weight (kg): the ratio of volume of buffer solution (L)=1:15 ~ 30, raw material dry powder is added in enzyme liquid;
(5) enzymolysis: after raw material dry powder and enzyme liquid are stirred, lucifuge insulation in the thermostat water bath of 50 ~ 55 DEG C, every 30min stirs once, enzymolysis 3-4h, generates the mixture of enzymolysis solution and residue;
(6) extract: 75 ~ 95% ethanol are added in above-mentioned enzymolysis solution and residue mixture by the method adding 75 ~ 95% ethanol of 15 ~ 30L by every kg raw material dry powder, under 60 ~ 65 DEG C of conditions, extract 2 ~ 3h, after filtration, obtain extracting solution;
(7) finished product: putting extracting solution in the baking oven of 60 ~ 70 DEG C dries to constant weight.
2. a kind of method utilizing purple corn to extract pycnogenols according to claim 1, is characterized in that by following steps group
Become:
(1) put fresh purple corn to dry in 65 DEG C of baking ovens, pulverize with high-speed tissue mashing machine, 40 orders sieve, and obtain raw material dry powder for subsequent use;
(2) get 0.lmol/L citric acid solution 160ml, 0.2mol/L disodium phosphate soln 320m1, mix and dilute and be settled to 1000ml, be mixed with buffered soln;
(3) get the buffered soln of 250ml, add 0.05g polygalacturonase and 0.075g cellulase, stir and make it dissolve completely, form uniform enzyme liquid;
(4) in above-mentioned enzyme liquid, add the raw material dry powder of 10g;
(5) lucifuge insulation in the thermostat water bath of 50 DEG C after stirring, every 30min stirs once, enzymolysis 3h, generates the mixture of enzymolysis solution and residue;
(6) in the mixture of enzymolysis solution and residue, add 250m195% ethanol, under 60 DEG C of conditions, extract 2h, after filtration extracting solution;
(7) putting extracting solution in the baking oven of 65 DEG C dries to constant weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510667778.2A CN105254606A (en) | 2015-10-16 | 2015-10-16 | Method for extracting procyanidins from purple corn |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510667778.2A CN105254606A (en) | 2015-10-16 | 2015-10-16 | Method for extracting procyanidins from purple corn |
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| CN105254606A true CN105254606A (en) | 2016-01-20 |
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| CN201510667778.2A Pending CN105254606A (en) | 2015-10-16 | 2015-10-16 | Method for extracting procyanidins from purple corn |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110467594A (en) * | 2019-08-22 | 2019-11-19 | 西安隆泽生物工程有限责任公司 | A kind of extraction process of Qarnet rice procyanidine |
| CN115260145A (en) * | 2022-09-13 | 2022-11-01 | 山东中红农业生物科技有限公司 | Extraction process of black corn anthocyanin |
-
2015
- 2015-10-16 CN CN201510667778.2A patent/CN105254606A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110467594A (en) * | 2019-08-22 | 2019-11-19 | 西安隆泽生物工程有限责任公司 | A kind of extraction process of Qarnet rice procyanidine |
| CN115260145A (en) * | 2022-09-13 | 2022-11-01 | 山东中红农业生物科技有限公司 | Extraction process of black corn anthocyanin |
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Application publication date: 20160120 |
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| WD01 | Invention patent application deemed withdrawn after publication |