CN105247067A - 融合蛋白酶 - Google Patents

融合蛋白酶 Download PDF

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Publication number
CN105247067A
CN105247067A CN201480029964.7A CN201480029964A CN105247067A CN 105247067 A CN105247067 A CN 105247067A CN 201480029964 A CN201480029964 A CN 201480029964A CN 105247067 A CN105247067 A CN 105247067A
Authority
CN
China
Prior art keywords
protease
fusion
protein
bifunctional fusion
fusion protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201480029964.7A
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English (en)
Chinese (zh)
Inventor
A.C.肖
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of CN105247067A publication Critical patent/CN105247067A/zh
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/485—Exopeptidases (3.4.11-3.4.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/14—Dipeptidyl-peptidases and tripeptidyl-peptidases (3.4.14)
    • C12Y304/14011—Xaa-Pro dipeptidyl-peptidase (3.4.14.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22028—Picornain 3C (3.4.22.28)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201480029964.7A 2013-05-24 2014-05-23 融合蛋白酶 Withdrawn CN105247067A (zh)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
EP13169173 2013-05-24
EP13169173.5 2013-05-24
EP13171191.3 2013-06-10
EP13171191 2013-06-10
US201361834100P 2013-06-12 2013-06-12
US61/834100 2013-06-12
EP13194053 2013-11-22
EP13194053.8 2013-11-22
US201361909412P 2013-11-27 2013-11-27
US61/909412 2013-11-27
PCT/EP2014/060696 WO2014187974A1 (en) 2013-05-24 2014-05-23 Fusion protease

Publications (1)

Publication Number Publication Date
CN105247067A true CN105247067A (zh) 2016-01-13

Family

ID=51932984

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480029964.7A Withdrawn CN105247067A (zh) 2013-05-24 2014-05-23 融合蛋白酶

Country Status (5)

Country Link
US (1) US20160122793A1 (https=)
EP (1) EP3004369A1 (https=)
JP (1) JP2016518855A (https=)
CN (1) CN105247067A (https=)
WO (2) WO2014187974A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110128540A (zh) * 2019-04-18 2019-08-16 江南大学 一种基于便携式血糖仪的广谱二抗
CN112011497A (zh) * 2020-08-28 2020-12-01 上海交通大学 分泌表达猪源性抗产肠毒素大肠杆菌k88菌毛单链抗体的重组乳酸乳球菌及其制备方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160060311A1 (en) 2014-08-27 2016-03-03 Daewoong Jo Development of Protein-Based Biotherapeutics That Penetrates Cell-Membrane and Induces Anti-Lung Cancer Effect - Improved Cell-Permeable Suppressor of Cytokine Signaling (iCP-SOCS3) Proteins, Polynucleotides Encoding the Same, and Anti-Lung Cancer Compositions Comprising the Same
CN110592057B (zh) * 2019-09-27 2022-01-28 昆明理工大学 嵌合裂解酶ILTphg和编码此酶的多核苷酸
US20250122489A1 (en) * 2023-10-16 2025-04-17 Zhenyu Zhu Protein Thermostability Reduction by Tag Fusion and Its Usage

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4769326A (en) * 1980-02-29 1988-09-06 The Regents Of The University Of California Expression linkers
CA1200773A (en) * 1980-02-29 1986-02-18 William J. Rutter Expression linkers
NZ236819A (en) * 1990-02-03 1993-07-27 Max Planck Gesellschaft Enzymatic cleavage of fusion proteins; fusion proteins; recombinant dna and pharmaceutical compositions

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110128540A (zh) * 2019-04-18 2019-08-16 江南大学 一种基于便携式血糖仪的广谱二抗
CN110128540B (zh) * 2019-04-18 2021-12-14 江南大学 一种基于便携式血糖仪的二抗
CN112011497A (zh) * 2020-08-28 2020-12-01 上海交通大学 分泌表达猪源性抗产肠毒素大肠杆菌k88菌毛单链抗体的重组乳酸乳球菌及其制备方法
CN112011497B (zh) * 2020-08-28 2022-06-07 上海交通大学 分泌表达猪源性抗产肠毒素大肠杆菌k88菌毛单链抗体的重组乳酸乳球菌及其制备方法

Also Published As

Publication number Publication date
US20160122793A1 (en) 2016-05-05
JP2016518855A (ja) 2016-06-30
WO2014187974A1 (en) 2014-11-27
WO2014187960A1 (en) 2014-11-27
EP3004369A1 (en) 2016-04-13

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C06 Publication
PB01 Publication
C10 Entry into substantive examination
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WW01 Invention patent application withdrawn after publication

Application publication date: 20160113

WW01 Invention patent application withdrawn after publication