CN105237120A - High-effective inonotus obliquus culture medium capable of effectively reducing cultivation period and preparation method thereof - Google Patents

Abstract

The invention discloses a high-effective inonotus obliquus culture medium capable of effectively reducing the cultivation period, wherein the culture medium includes the following components, by weight, cytokinin, kinetin, auxin, tranexamic acid, ferulic acid, chitosan microspheres, plant sponge, straw, bean curd residue, monopotassium phosphate, high-calcium bone meal, yeast and zinc lactate. In the invention, the cytokinin, the kinetin, the auxin, the tranexamic acid and the ferulic acid are supported by the chitosan in an adsorption manner, so that division and growth of the inonotus obliquus is effectively controlled, thereby reducing cultivation period of the inonotus obliquus. The tranexamic acid and the ferulic acid effectively improve activity of extracellular enzyme of the inonotus obliquus, so that further decomposition and utilization of the inonotus obliquus to the culture medium is promoted, thereby achieving high bio-value conversion rate. During a fermentation preparation process of the culture medium, the yeast, which is supported by the plant sponge, is used for regulating the activity and release time of the yeast, so that decomposition of a fermentation substrate and abundance and balance of a fermentation product are promoted, thereby providing fundament to high-effective and high-quality cultivation of the inonotus obliquus.

Description

A kind ofly effectively can shorten efficient culture medium of the fine pore fungi cultivation period of birch and preparation method thereof

Technical field

The present invention relates to culture medium of edible fungus field, particularly relate to and a kind ofly effectively can shorten efficient culture medium of the fine pore fungi cultivation period of birch and preparation method thereof.

Background technology

The fine pore fungi of birch is a kind of very precious and famous and precious medicinal fungi, can the various diseases such as anti-curing cancers, hypertension, tumour.But the fine pore fungi mycelial growth of birch is relatively long to the cycle forming sclerotium, along with the nutrition needed in absorption substratum is to meet needed for himself, edible mushrooms produces extracellular enzyme in growth and development process, extracellular enzyme can decompose substratum, make polysaccharide, protein, the natural high polymers such as nucleic acid are cracked into the small-molecule substance that hypha of edible fungus and sporophore are convenient to absorb, for hypha of edible fungus growth, former base is formed, sporophore growth is grown provides nutritive substance, the shortening realizing the fine pore fungi growth cycle of birch with active regulation and control can be produced by controlling the main extracellular enzyme of the fine pore fungi of birch, realize the high quality cultivation of the fine pore fungi of birch simultaneously, and then realize the high quality and high efficiency cultivation of the fine pore fungi of birch.

Summary of the invention

The present invention is directed to the Efficient Cultivation demand of the fine pore fungi of birch, provide a kind of and effectively can shorten efficient culture medium of the fine pore fungi cultivation period of birch and preparation method thereof.

The present invention is achieved by the following technical solutions:

A kind of efficient culture medium that effectively can shorten the fine pore fungi cultivation period of birch, comprise following parts by weight of component: phytokinin 0.02-0.03, kinetin 0.01-0.02, growth hormone 0.03-0.04, Trenaxmine 0.4-0.5, forulic acid 0.7-0.8, low complexing organic copper 0.3-0.4, chitosan microball 3.4-3.6, plant sponge 3-4, straw 70-72, apple residue 40-44, Exocarpium cocois (Cocos nucifera L) 38-40, bagasse 58-60, residue from beans after making 60-62, mulberries albumen 30-32, animal feather hydrolysis powder 18-19, potassium primary phosphate 0.4-0.5, phoxim emulsifiable concentrate 0.1-0.2, sodium lignosulfonate 0.2-0.3, high calcium bone meal 4-5, yeast 2-3, zinc lactate 0.3-0.4, calcium lactate 0.1-0.2, nicotinic acid 4-5, chelating amino acids manganese 0.01-0.02, methionine(Met) 0.9-1, VITAMIN 0.3-0.4, Nucleotide 0.2-0.3, appropriate water.

Effectively can shorten an efficient culture medium preparation method for the fine pore fungi cultivation period of birch, comprise the following steps:

(1) by plant sponge, half yeast, Nucleotide mixing, and add gross weight 2-3 water doubly, moderate-speed mixer 2-3h, the zymophyte obtaining plant sponge absorption loading is for subsequent use; By chitosan microball, nicotinic acid and 1-1.5 water mixing and stirring doubly, at 90-95 DEG C, heated and stirred 1.5-2h dissolves to chitosan microball afterwards, chitosan microball must be activated for subsequent use, chitosan microball, phytokinin, kinetin, growth hormone, Trenaxmine, forulic acid mixing will be activated, low temperature moderate-speed mixer 32-35min, the promotion conditioning agent that must activate chitosan microball loading is for subsequent use;

(2) straw, apple residue, Exocarpium cocois (Cocos nucifera L), bagasse mixing are put into pulverizer and pulverized 1.5-2h, put into bulking machine afterwards, puffing 20-30min, obtain composite puffed comminuted powder for subsequent use;

(3) by low complexing organic copper, potassium primary phosphate, phoxim emulsifiable concentrate, sodium lignosulfonate, zinc lactate, calcium lactate, chelating amino acids manganese, methionine(Met), VITAMIN mixing, and add gross weight 3-4 water doubly, at 30-32 DEG C, heated and stirred is to evenly, obtains nutritive medium for subsequent use;

(4) the promotion conditioning agent composite puffed comminuted powder, residue from beans after making, mulberries albumen, high calcium bone meal, animal feather hydrolysis powder, activation chitosan microball loaded, nutritive medium mixing, and add gross weight 1-1.5 water mixing and stirring doubly, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, anaerobic fermentation 9-10d at 22-24 DEG C, obtains fermented liquid one for subsequent use; Second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte that the plant sponge absorption of access activation loads, anaerobic fermentation 9-10d at 19-20 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, sealed fermenting 28-30d at 21.5-22 DEG C, during the fermentation, every 2-3d stirs once, stirs 30-40min at every turn, obtains fermention medium for subsequent use;

(5) fermention medium is poured out fermentor tank, spread airing 3-10d out, adjusting moisture is afterwards the 58-60% of gross weight, adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 20-24h, to obtain final product.

Advantage of the present invention is:

The phytokinin that the present invention adopts chitosan absorption to load, kinetin, growth hormone, Trenaxmine, forulic acid effectively control the merisis of the fine pore fungi of birch, shorten the cultivation period of the fine pore fungi of birch; Employing Trenaxmine, forulic acid effectively improve the activity of the fine pore fungi extracellular enzyme of birch, and be conducive to the further decomposition of the fine pore fungi of birch to substratum and utilize, realize high biological value transformation efficiency, the high-effect high-quality realizing the fine pore fungi of birch is cultivated; In substratum fermentation preparation process, the zymophyte adopting plant sponge absorption to load regulates and controls saccharomycetic activity and time of releasing, extend fermentation time, facilitate the decomposition of fermentation substrate and the abundant equilibrium of tunning, for the high-effect high-quality cultivation of the fine pore fungi of birch provides the foundation.

Embodiment

A kind of efficient culture medium that effectively can shorten the fine pore fungi cultivation period of birch, comprise following parts by weight of component: phytokinin 0.02, kinetin 0.01, growth hormone 0.03, Trenaxmine 0.4, forulic acid 0.7, low complexing organic copper 0.3, chitosan microball 3.4, plant sponge 3, straw 70, apple residue 40, Exocarpium cocois (Cocos nucifera L) 38, bagasse 58, residue from beans after making 60, mulberries albumen 30, animal feather hydrolysis powder 18, potassium primary phosphate 0.4, phoxim emulsifiable concentrate 0.1, sodium lignosulfonate 0.2, high calcium bone meal 4, yeast 2, zinc lactate 0.3, calcium lactate 0.1, nicotinic acid 4, chelating amino acids manganese 0.01, methionine(Met) 0.9, VITAMIN 0.3, Nucleotide 0.2, appropriate water.

Effectively can shorten an efficient culture medium preparation method for the fine pore fungi cultivation period of birch, comprise the following steps:

(1) by plant sponge, half yeast, Nucleotide mixing, and add the water of gross weight 2 times, moderate-speed mixer 2h, the zymophyte obtaining plant sponge absorption loading is for subsequent use; By the water mixing and stirring of chitosan microball, nicotinic acid and 1 times, at 90 DEG C, heated and stirred 1.5h dissolves to chitosan microball afterwards, chitosan microball must be activated for subsequent use, chitosan microball, phytokinin, kinetin, growth hormone, Trenaxmine, forulic acid mixing will be activated, low temperature moderate-speed mixer 32min, the promotion conditioning agent that must activate chitosan microball loading is for subsequent use;

(2) straw, apple residue, Exocarpium cocois (Cocos nucifera L), bagasse mixing are put into pulverizer and pulverized 1.5h, put into bulking machine afterwards, puffing 20min, obtain composite puffed comminuted powder for subsequent use;

(3) by low complexing organic copper, potassium primary phosphate, phoxim emulsifiable concentrate, sodium lignosulfonate, zinc lactate, calcium lactate, chelating amino acids manganese, methionine(Met), VITAMIN mixing, and add the water of gross weight 3 times, at 30 DEG C, heated and stirred is to evenly, obtains nutritive medium for subsequent use;

(4) the promotion conditioning agent composite puffed comminuted powder, residue from beans after making, mulberries albumen, high calcium bone meal, animal feather hydrolysis powder, activation chitosan microball loaded, nutritive medium mixing, and add the water mixing and stirring of gross weight 1 times, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, anaerobic fermentation 9d at 22 DEG C, obtains fermented liquid one for subsequent use; Second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte that the plant sponge absorption of access activation loads, anaerobic fermentation 9d at 19 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, sealed fermenting 28d at 21.5 DEG C, during the fermentation, every 2d stirs once, stirs 30min at every turn, obtains fermention medium for subsequent use;

(5) fermention medium is poured out fermentor tank, spread airing 3d out, adjusting moisture is afterwards 58% of gross weight, and adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 20h, to obtain final product.

Claims (2)

1. one kind effectively can be shortened the efficient culture medium of the fine pore fungi cultivation period of birch, it is characterized in that, comprise following parts by weight of component: phytokinin 0.02-0.03, kinetin 0.01-0.02, growth hormone 0.03-0.04, Trenaxmine 0.4-0.5, forulic acid 0.7-0.8, low complexing organic copper 0.3-0.4, chitosan microball 3.4-3.6, plant sponge 3-4, straw 70-72, apple residue 40-44, Exocarpium cocois (Cocos nucifera L) 38-40, bagasse 58-60, residue from beans after making 60-62, mulberries albumen 30-32, animal feather hydrolysis powder 18-19, potassium primary phosphate 0.4-0.5, phoxim emulsifiable concentrate 0.1-0.2, sodium lignosulfonate 0.2-0.3, high calcium bone meal 4-5, yeast 2-3, zinc lactate 0.3-0.4, calcium lactate 0.1-0.2, nicotinic acid 4-5, chelating amino acids manganese 0.01-0.02, methionine(Met) 0.9-1, VITAMIN 0.3-0.4, Nucleotide 0.2-0.3, appropriate water.

2. effectively can shorten an efficient culture medium preparation method for the fine pore fungi cultivation period of birch, it is characterized in that, comprise the following steps:

(1) by plant sponge, half yeast, Nucleotide mixing, and add gross weight 2-3 water doubly, moderate-speed mixer 2-3h, the zymophyte obtaining plant sponge absorption loading is for subsequent use; By chitosan microball, nicotinic acid and 1-1.5 water mixing and stirring doubly, at 90-95 DEG C, heated and stirred 1.5-2h dissolves to chitosan microball afterwards, chitosan microball must be activated for subsequent use, chitosan microball, phytokinin, kinetin, growth hormone, Trenaxmine, forulic acid mixing will be activated, low temperature moderate-speed mixer 32-35min, the promotion conditioning agent that must activate chitosan microball loading is for subsequent use;

(2) straw, apple residue, Exocarpium cocois (Cocos nucifera L), bagasse mixing are put into pulverizer and pulverized 1.5-2h, put into bulking machine afterwards, puffing 20-30min, obtain composite puffed comminuted powder for subsequent use;

(3) by low complexing organic copper, potassium primary phosphate, phoxim emulsifiable concentrate, sodium lignosulfonate, zinc lactate, calcium lactate, chelating amino acids manganese, methionine(Met), VITAMIN mixing, and add gross weight 3-4 water doubly, at 30-32 DEG C, heated and stirred is to evenly, obtains nutritive medium for subsequent use;

(4) the promotion conditioning agent composite puffed comminuted powder, residue from beans after making, mulberries albumen, high calcium bone meal, animal feather hydrolysis powder, activation chitosan microball loaded, nutritive medium mixing, and add gross weight 1-1.5 water mixing and stirring doubly, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, anaerobic fermentation 9-10d at 22-24 DEG C, obtains fermented liquid one for subsequent use; Second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte that the plant sponge absorption of access activation loads, anaerobic fermentation 9-10d at 19-20 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, sealed fermenting 28-30d at 21.5-22 DEG C, during the fermentation, every 2-3d stirs once, stirs 30-40min at every turn, obtains fermention medium for subsequent use;

(5) fermention medium is poured out fermentor tank, spread airing 3-10d out, adjusting moisture is afterwards the 58-60% of gross weight, adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 20-24h, to obtain final product.