CN105235253B - Biological chip eluting device - Google Patents

Abstract

The invention relates to a biological chip eluting device. Biomass chips are flowing-through type chips. The biological chip eluting device comprises a containing piece and a piston piece. The containing piece comprises a bottom wall and a side wall surrounding the bottom wall. Installing holes for arranging the biological chips are formed in the bottom wall of the containing piece in a penetrating manner. The piston piece can stretch into the containing piece and is in movable sealing contact with the side wall, and a pressing cavity is formed among the piston piece, the bottom wall and the side wall. The piston piece can move towards the bottom wall so that the pressure in the pressing cavity can be increased, and fluid in the flowing-through type chips can flow out. By means of the biological chip eluting device, liquid on the biological chips can be effectively removed.

Description

Biochip washing device

Technical field

The present invention relates to biochip field, more particularly to a kind of biochip washing device.

Background technology

Biochip referred to by microelectronics, micro-processing technology, the miniature biochemical system built on solid substrate, To realize detecting cell, DNA, RNA, protein, polypeptide or other biological components accurately, quickly, in large quantities.

In the detection process of biochip, being substantially all can have solution reaction step.And in solution reaction step knot Shu Hou, is required to carry out eluting to biochip, by biochip detection liquid or be attached on biochip other Liquid is got rid of.With the fast development of biotechnology, the also more and more higher of the requirement to the washing device of biochip.

In the prior art, generally detection liquid or other liquid being attached on biochip cannot effectively be gone Remove, liquid residue is easily produced on chip.In ensuing detection, the liquid of this residual will be produced to testing result Raw large effect.

Accordingly, it would be desirable to a kind of device of the liquid that can effectively remove on biochip.

The content of the invention

For the problems referred to above, the present invention proposes a kind of biochip washing device, is filled using this biochip eluting Put the liquid that can effectively remove on biochip.

A kind of biochip washing device is proposed according to the present invention, biochip is to flow through formula chip, it is characterised in that Biochip washing device includes：Receiving member, receiving member includes diapire and surrounds the side wall of diapire, passes through on the diapire of receiving member The formula of wearing is configured with the installing hole for arranging biochip, and the piston piece that can be extend in receiving member, piston piece and side wall Movable sealed is contacted, and pressure chamber is formed between piston piece, diapire and side wall, wherein, piston piece can move to increase towards diapire The pressure of big pressure intracavity.

By the biochip washing device of the present invention, biochip can be arranged in installing hole.Now, indent is substantially Closing is completely enclosed.When piston piece is moved towards diapire, the pressure for pressing intracavity rises.In normal state, formula core is flow through The bottom of piece is closing, effectively to contain liquid.And when the pressure for pressing intracavity is increased in certain limit, flow through formula chip Bottom is opened, so as to make liquid leave chip, thus, it is possible to effectively take out the liquid on biochip, to realize to biochip Eluting.Easily liquid can be extruded by this structure, be not likely to produce the residual of liquid, thereby ensure that subsequent detection Accuracy.

In one embodiment, installing hole is multiple, and each installing hole can selectively opened or closing.It is many by arranging Individual installing hole can simultaneously install multiple biochips, and thus, it is possible to while carrying out eluting to multiple biochips.In addition, being not required to When using wherein one or more installing holes, installing hole can be closed to ensure to press the pressure energy of intracavity smoothly to increase.

In one embodiment, force side is configured with one end for dorsad pressing chamber of piston piece, by applying to force side Plus-pressure can push piston piece makes piston piece move towards diapire.Work can be conveniently and effectively made by carrying out pressure to force side Plug member is moved.

In one embodiment, piston piece dorsad presses chamber to extend so that force side is reached outside the wall of side, and force side is hung down The straight size in the direction of motion is more than the size that the opening that side wall is formed is perpendicularly to the direction of movement.This structure facilitates user Force side is manipulated.

In one embodiment, diapire and side wall detachable connection.So as to be easily removed biochip.

In one embodiment, biochip washing device also includes surrounding the housing of receiving member, and housing includes being arranged at The drain pan wall of the side of the dorsad piston piece of diapire, and be connected with drain pan wall and surround the side shell wall of side wall, drain pan wall with It is closing in the corresponding region of installing hole.By arranging this housing, biochip washing device can be placed into water-bath Incubated in pot or in drying baker.

In one embodiment, opening is configured with the region corresponding with installing hole of drain pan wall towards installing hole Groove, the biochip being inserted in installing hole can contact with the surface of groove.Can be more uniform and fast by this structure Fast ground is incubated to biochip.

In one embodiment, on diapire towards drain pan wall be extended have pin, drain pan wall not with installing hole The jack corresponding with pin is configured with corresponding region, pin can be inserted in jack.Inserted by inserting the prongs within In hole, receiving member can be stably maintained in housing.

In one embodiment, dorsad receiving member is extended with foot on drain pan wall, and jack is extended in foot.It is logical Cross together with this structure further stably can be stably bound in kerve with receiving member, and cause biochip eluting dress Putting can more be stably placed in platform or plane.

In one embodiment, side wall includes the first side wall being fixedly linked with diapire, and detachable with the first side wall The second sidewall that formula is tightly connected.The first side wall can be made when biochip is placed in installing hole by this structure Separate with second sidewall, and without separating piston piece and the side wall of receiving member, be thus avoided that piston piece and receiving member Between regular fractionation and connection, so as to the stability of sealed contact between piston piece and second sidewall has been effectively ensured, Further can effectively increase the pressure of pressure intracavity when piston piece is moved towards receiving member.

In one embodiment, first seal is configured with the edge towards second sidewall of the first side wall, The edge towards the first side wall of two side walls is configured with second seal, and first seal and second seal contact to incite somebody to action The first side wall and second sidewall are sealingly attached to together.

Compared with prior art, it is an advantage of the current invention that：(1) biochip can be arranged in installing hole.(2) work is worked as Plug member towards receiving member diapire move when, press intracavity pressure rise so that the liquid in biochip can be extruded effectively. Easily liquid can be extruded by this structure, be not likely to produce the residual of liquid, thereby ensure that the accuracy of subsequent detection. (3) this biochip washing device is easy to operate, and motility is high, and elution efficiency is high, and biochip can be carried out to wash in batches It is de-.

Description of the drawings

Hereinafter the present invention will be described in more detail based on embodiment and refer to the attached drawing.Wherein：

Fig. 1 is showing the structural representation of one embodiment of the biochip washing device of the present invention.

Fig. 2 is showing the structural representation of one embodiment of the installing hole of the biochip washing device of Fig. 1.

Fig. 3 is showing the structural representation of another embodiment of the installing hole of the biochip washing device of Fig. 1.

Fig. 4 is showing the structural representation of one embodiment of the base of the biochip washing device of the present invention.

Fig. 5 is showing the structural representation of another embodiment of the biochip washing device of the present invention.

In the accompanying drawings, identical part uses identical reference.Accompanying drawing is not drawn according to actual ratio.

Specific embodiment

Below in conjunction with accompanying drawing, the invention will be further described.

Fig. 1 schematically illustrates the general structure of biochip washing device 1 (hereinafter referred to as device 1).

Device 1 includes receiving member, and receiving member is configured with diapire 11, and is arranged on diapire 11 and surrounds the side wall of diapire. As shown in figure 1, being configured with installing hole 111 on the diapire 11 of receiving member.Biochip can be arranged in installing hole 111, with Fixed biochip.Meanwhile, side wall energy surrounds the biochip being arranged in installing hole 111, plays certain to biochip Protective effect.For example, biochip here can be protein chip or gene chip etc..Here diapire can be porous Test platform.

Again, it should be noted that ground is, biochip here should be and flow through formula chip.Formula chip is flow through in normal laying state The lower bottom with closing to carry liquid (for example, detecting liquid), and when by certain pressure bottom form opening with Liquid is flowed out.Its bearing part can be made up of nylon membrane, nitrocellulose filter etc..For example, HPV gene chips.This It is well known to the skilled artisan in the art that kind flows through formula chip, and here is not added with repeating.

Device 1 also includes the piston piece 20 with the sealed contact of side wall of receiving member, and piston piece 20 can be in the guiding of side wall Under, move in receiving member.Piston piece 20 is relative with diapire 11, so as to form pressure chamber between piston piece, diapire and side wall 17。

Moved towards the diapire of receiving member by making piston piece 20, the volume in pressure chamber 17 can be reduced.Because pressure is for substantially It is sealing or sealing, therefore press the reduction of the volume of chamber 17 to increase the pressure pressed in chamber 17.When piston piece 20 is towards diapire 11 After moving a certain distance, pressing the pressure in chamber 17 to increase to can form the degree of opening in the bottom for flowing through formula chip, thus make The liquid that must be carried in biochip can be squeezed out effectively under pressure, so as to biochip eluting is clean, And cause to be not in liquid residue on biochip.By using the biochip energy side after the eluting of device 1 of the present invention Just after using in detection, and the accuracy of testing result can be effectively ensured.It is more simple to the mode of operation of this device 1 It is single, so as to conveniently efficiently carry out eluting to biochip.Meanwhile, it is various anti-if necessary to add successively in container 4 Answering the removal of liquid, each reactant liquor can then avoid each reactant liquor in removal process also without carrying out by other transfer vessels In cross-contamination.

As shown in figure 1, installing hole 111 can be for multiple, multiple installing holes 111 are spaced from each other setting.Installing hole 111 can be such as Rectangular array arrangement as shown in Fig. 1, installs also dependent on needing to be arranged with other regular or irregular arrangement modes Hole 111.By arranging biochip in multiple installing holes 111, can realize carrying out eluting to multiple biochips simultaneously, be Say, batch eluting can be carried out to biochip.

In the case where multiple installing holes are provided with, installing hole can be configured to selectively opened or closing.Thus, When needing using one or more of installing holes to arrange biochip, the installing hole of respective numbers need to be only opened, and Keep other installing hole closings.Thus, can prevent gas from leaking out from other installing holes, so as to can guarantee that the air pressure of pressure intracavity Can smoothly increase.

Fig. 2 and Fig. 3 schematically illustrate the concrete structure of installing hole 111.Installing hole 111 is through hole, so that biological core Liquid in piece can successfully be expressed into the downside of diapire 11 along the direction of motion.Can effectively be prevented by the device of this structure The reactant liquor stream that the biochip being only disposed therein in an installing hole 111 is carried to other installing holes 111 are carried In biochip, the cross-contamination between biochip is thus prevent.

In addition, the bottom of biochip can also pass through installing hole 111 and reach the downside of diapire 11, thus, it is possible to further side Just liquid is discharged.

Wherein, the diapire 11 shown in Fig. 2 is provided with the region of installing hole 111 (i.e. dorsad piston piece downward at it Direction) extend, more to surround and protect biochip.In addition, the diapire 11 shown in Fig. 3 is tabular, so as to biology Chip can easily extend to the downside of diapire 11, be discharged liquid with facilitating.

As needed, installing hole 111 here is it is so structured that circular port, square opening, tri-angle-holed, slotted eye, prunus mume (sieb.) sieb.et zucc. Flower-shape hole or the hole of other arbitrarily suitable shapes.

Here it should be understood that diapire can construct what is be integrated, installing hole is formed with thereon.In addition, diapire can also It is split type, it includes the coupling part being connected with side wall, and the installation orifice plate with the coupling part detachable connection Body, installing hole is formed on installing hole plate body.Here it should be understood that different installing hole plate bodys can be provided with varying number, Different shape, different size of installing hole, so as to pass through replacement installing hole plate body quantity, the shape of installing hole can be easily changed And size.

In one embodiment, detachable connection between diapire 11 and side wall.Can be easy to by this setup Ground is changed to diapire 11, easily it is cleaned and be safeguarded.

Especially, biochip washing device 1 can be made to include multiple diapires 11.In this case, different diapires can It is provided with the installing hole of different shape, size.Thus, one of them suitable diapire may be selected and (alternatively selects what is be adapted to Diapire) to be connected with side wall, the installing hole used required for thus, it is possible to select.

It should be understood that also first can will flow through formula chip and arrange to non-enclosed chip reactive tank as shown in Figure 5 In 4, then the non-enclosed chip reactive tank 4 that flows through formula chip will be carried arrange in installing hole.Non-enclosed chip reaction The substructure of groove 4 has osmosizing portion 6.In normal state (for example at ambient pressure), osmosizing portion 6 is closed state, thus, it is possible to Liquid (such as reactant liquor or detection liquid) is set in non-enclosed chip reactive tank 4.And the pressure in pressure intracavity increases to one After determining degree, osmosizing portion 6 cannot again carry the liquid in non-enclosed chip reactive tank 4, so that thus liquid can locate stream Go out, chip is not in the environment with liquid.

Preferably, osmosizing portion 6 can be made up of nylon membrane, nitrocellulose filter etc..

Preferably, the area of section of the lower end of non-enclosed chip reactive tank is less than the area of section of upper end, so that non-envelope Enclosed chip reactive tank 4 can be extended downwardly partially across installing hole 111.It is, non-enclosed chip can be reacted Groove 4 is arranged on installing hole 111.In installation process, the lower end of non-enclosed chip reactive tank 4 passes through installing hole 111, rather than The upper end of closed chip reactive tank 4 is limited at the upside of installing hole 111.By this setup, can be easily Non-enclosed chip reactive tank 4 is installed and pulled down, the reaction of biochip and the carrying out of elution action is facilitated.

In order to be able to non-enclosed chip reactive tank 4 is conveniently placed on installing hole 111, for example, non-enclosed chip Reactive tank 4 is so structured that the structure with enlarging, to be stuck at installing hole 111.But non-enclosed chip reactive tank 4 is not limited In this version, other versions are may be configured to.For example, the upper end open of non-enclosed chip reactive tank 4 Place is configured with the outer (not shown) for extending radially outwardly, to be overlapped on the outer at installing hole 111.Or non-close The outer wall of formula chip reactive tank 4 has taper, so that non-enclosed chip reactive tank 4 is stuck at installing hole 111.Thus, only If enabling the structure that non-enclosed chip reactive tank 4 is simply and easily installed at installing hole 111 to fall into the present invention's Protection domain.

As shown in figure 1, the side wall of receiving member includes the first side wall 12 being fixedly linked with diapire 11, and with piston piece 20 The second sidewall 13 of sealed contact, the first side wall and second sidewall detachable connection.The first side wall 12 can be remained around Around installing hole, and thus, it is possible to when biochip is arranged in installing hole, protect biochip.Further, since second sidewall 13 it is sealed with piston piece 20 contact and can disassemble with the first side wall, therefore by biochip arrange in installing hole When, or when biochip is taken out in installing hole, it may not be necessary to second sidewall is split with piston piece, and is to maintain piston piece With the state of the sealed contact of side wall.Thus, regular fractionation and the assembling that can be prevented effectively between piston piece and receiving member, from And the stability of sealed contact between piston piece and second sidewall has been effectively ensured, and then be effectively ensured in piston piece direction When the diapire of receiving member is moved, press the pressure energy effectively increase of intracavity and the liquid that biochip is carried is extruded.

As needed, the first side wall 12 and second sidewall 13 can be respectively configured to cylindricality, taper, infundibulate or other are any The side wall of suitable shape, while, it is ensured that the first side wall 12 is mutually adapted to be assembled together with the shape of second sidewall 13. Diapire can be configured to circular, square or other arbitrarily suitable shapes, while need to ensure that diapire is mutually fitted with the shape of the first side wall Should.

Sealing member is also configured with the contact position of the first side wall and second sidewall, so that the first side wall energy and second sidewall Be sealingly attached to further ensure together the pressure energy effectively increase of pressure intracavity and so that the liquid that carried of biochip Can be extruded.As shown in figure 1, first seal 14 can be constructed in the edge towards second sidewall of the first side wall, while The edge towards the first side wall of second sidewall constructs second seal 15.It is equal in first seal 14 and second seal 15 Can be configured to substantially be in bowl-shape sealing ring, the bowl-shape opening of first seal and the bowl-shape opening of second seal are relative. Make the first side wall near second sidewall when, first seal and second seal can mutually crimps so that first seal With the air pressure outside second seal more than the air pressure in bowl-shape opening, so as to close effectively by first seal and second seal It is enclosed in together, and is achieved in being tightly connected for the first side wall and second sidewall.

As shown in figure 1, being configured with force side 30 in the side in chamber 17 of dorsad pressing of piston piece 20.Can be by force side 30 It is upper to apply pressure and promote piston piece 20, piston piece 20 is moved towards diapire 11.Here it should be understood that people can directly be passed through Power to force side 30 presses, and also force side can be pressed by other machinerys.

Preferably, force side 30 is outside receiving member.As shown in figure 1, overthe openings of the force side in receiving member.Apply The size being perpendicularly to the direction of movement at power end 30 is more than the size that the opening that the side wall of receiving member is formed is perpendicularly to the direction of movement.This Plant structure to cause when exerting a force to pressure end, piston piece can be moved to the side wall of force side and receiving member towards the diapire of receiving member Contact.Now, because the size of force side is larger, therefore when force side is pushed, force side can only be with receiving member side wall Upper edge contacts, and cannot be introduced in receiving member, so as to limit the motion of piston piece so as to cannot be re-directed towards receiving member Diapire is moved.

Preferably, can make the length along the direction of motion of piston piece less than receiving member side wall along the direction of motion length. So, along when contacting on side wall of the force side with receiving member, piston piece cannot also touch the diapire of receiving member, thus effectively Piston piece 20 is prevented to contact with biochip, so as to prevent biochip to be contaminated, while preventing biological core Piece is squeezed.

Here it should be understood that should ensure that the shape of piston piece 20, the especially shape of force side 30 and the first side wall 12 It is adapted with effectively eluting chip with the shape of second sidewall 13.

In addition, device may also include housing 40 as shown in Figure 4.Housing 40 is configured with drain pan wall 41, and drain pan wall 41 is arranged In the side of the dorsad piston piece 20 of diapire 11, the region corresponding with installing hole 111 of drain pan wall 41 is closing.Housing is also It is configured with the side shell wall 42 for surrounding drain pan wall 41.Side shell wall 42 forms the space that can accommodate receiving member together with drain pan wall 41. When needing to carry out incubation reaction to biochip, housing can be enclosed within outside receiving member, and the device 1 with housing is put into Incubated in the device such as water-bath or drying baker.The setting of housing effectively prevent the stream in the devices such as water-bath or drying baker Body directly contacts with biochip.The biochip being inserted in installing hole 111 preferably contacts with drain pan wall 41, so as to The efficiency that heat is transmitted to biochip is enhanced, the efficiency of incubation reaction is which thereby enhanced.

Here it should be understood that in the case where housing 40 is provided with, can directly in this biochip washing device 1 Carry out the operation of biochip.That is, in this case, biochip washing device 1 is biochip reaction And washing device.Now, can effectively realize after biochip reaction, easily eluting be carried out to biochip.And can realize After eluting, directly operation is carried out to biochip in this device.So reaction repeatedly and elution action can be realized, So that greatly facilitating the carrying out of the process such as test or experiment.

It is further preferred that as shown in Figure 4, the groove 411 being open towards installing hole is configured with drain pan wall 41. The position of groove is corresponding with the position of installing hole, and the shape of groove is adapted with the shape of biochip bottom.To When biochip is inserted in installing hole, biochip can be plugged in groove and fit with the surface of groove.Thus, entering During row incubation reaction, temperature can be more uniformly transmitted on biochip, to improve the effectiveness of incubation reaction.

Here it should be understood that when base plate 11 is configured to structure as shown in Figure 3, moreover it is possible to convenient by biochip It is inserted in groove.In addition, drain pan wall 41 (especially it arranges fluted 411 part) here can be by resilient material Make, so that biochip preferably can contact with the surface of groove.

In addition, in order to the combination for ensureing housing and receiving member is relatively stable, pin 16 can be also configured with diapire 11, together When corresponding jack 44 is configured with drain pan wall 41.Pin 16 can be inserted in jack 44, to prevent receiving member and housing from sending out It is raw to relatively rotate.In addition, pin can also with jack interference fit, to prevent receiving member from unpredictably deviating from from housing.Pin Preferably several (for example, four), so that the combination between housing and receiving member is more stable.

Foot 43 can be also configured with drain pan wall 41.Foot 43 preferably several (for example, four).Housing can lead to Cross foot stably to stand in platform or plane, it is convenient that incubation reaction is carried out to biochip.

In addition, jack may extend in foot 43.So as to when pin 16 is inserted in jack, actually will Pin 16 is inserted in drain pan wall 41 and foot 43.By this structure, can be more easily embedding by receiving member and housing It is combined, and further improves the structural stability of device 1.

As needed, pin 16 can be configured to rod as shown in Figure 4 or other arbitrarily suitable shapes.The structure of jack 44 Make the shape being adapted with pin 16.In addition, foot 43 be configured to also dependent on needs rod as shown in Figure 4 or its The shape that he is arbitrarily adapted to.In the case where pin 16 needs to be inserted in foot 43, need to ensure that foot has can accommodate The size of pin.

Said apparatus 1 can efficiently and easily remove the liquid carried on biochip, thus, it is possible to the biology is effectively ensured The verity and effectiveness of the result in chip detection afterwards.

Although by reference to preferred embodiment, invention has been described, in the situation without departing from the scope of the present invention Under, various improvement can be carried out to it and part therein can be replaced with equivalent.Especially, as long as there is no structure punching Prominent, the every technical characteristic being previously mentioned in each embodiment can combine in any way.The invention is not limited in text Disclosed in specific embodiment, but including all technical schemes for falling within the scope of the appended claims.

Claims (9)

1. a kind of biochip washing device, the biochip is to flow through formula chip, it is characterised in that the biochip is washed De- device includes：

Receiving member, the receiving member includes diapire and surrounds the side wall of the diapire, the penetration type on the diapire of the receiving member The installing hole for arranging biochip is configured with, and

The piston piece that can be extend in the receiving member, the piston piece is contacted with the side wall movable sealed, in the work Pressure chamber is formed between plug member, diapire and side wall,

Wherein, the piston piece can be moved to increase the pressure of the pressure intracavity towards the diapire,

The side wall includes the first side wall being fixedly linked with the diapire, and second with the sealed contact of the piston piece Side wall, the first side wall and the second sidewall detachable-type sealed connect.

2. biochip washing device according to claim 1, it is characterised in that described in the direction in the first side wall The edge of second sidewall is configured with first seal, constructs in the edge of the direction of the second sidewall the first side wall There is second seal,

The first seal and second seal contact to be sealingly attached to the first side wall and second sidewall together.

3. biochip washing device according to claim 1 and 2, it is characterised in that the installing hole is multiple, each institute Stating installing hole can each selectively opened or closing.

4. biochip washing device according to claim 1, it is characterised in that in the dorsad described pressure of the piston piece Force side is configured with the one end in chamber,

The piston piece is set to move towards the diapire by pushing the piston piece to force side applying pressure energy.

5. biochip washing device according to claim 4, it is characterised in that the piston piece dorsad prolong by the pressure chamber Stretch so that the force side is reached outside the side wall, the size being perpendicularly to the direction of movement of the force side is more than the side The size that the opening that wall is formed is perpendicularly to the direction of movement.

6. biochip washing device according to claim 1, it is characterised in that the diapire and the detachable company of side wall Connect.

7. biochip washing device according to claim 1, it is characterised in that also including the shell for surrounding the receiving member Body, the housing include be arranged at the diapire dorsad described piston piece side drain pan wall, and with the drain pan wall It is connected and surrounds the side shell wall of the side wall,

The drain pan wall is closing in the region corresponding with the installing hole.

8. biochip washing device according to claim 7, it is characterised in that in the drain pan wall and the installation The groove being open towards the installing hole is configured with the corresponding region in hole,

The biochip being inserted in installing hole can contact with the surface of the groove.

9. the biochip washing device according to claim 7 or 8, it is characterised in that towards described on the diapire Drain pan wall is extended pin,

The jack corresponding with the pin is configured with the not region corresponding with the installing hole of the drain pan wall,

The pin can be inserted in the jack.