CN209222159U - A kind of compound micro-fluidic chip of array PDMS- paper base for single cell analysis - Google Patents
A kind of compound micro-fluidic chip of array PDMS- paper base for single cell analysis Download PDFInfo
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- CN209222159U CN209222159U CN201822017124.7U CN201822017124U CN209222159U CN 209222159 U CN209222159 U CN 209222159U CN 201822017124 U CN201822017124 U CN 201822017124U CN 209222159 U CN209222159 U CN 209222159U
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Abstract
The utility model relates to a kind of compound micro-fluidic chips of array PDMS- paper base for single cell analysis, are disposed with PDMS layer, glue-line, paper substrate and heat patch from top to bottom；The capture array being made of more than one concave structure is provided in the PDMS layer；The paper substrate includes the array being made of the hydrophilic matrix unit including hydrophilic paper base structure and hydrophobic structure；Each concave structure each of in the paper substrate in hydrophilic matrix unit and the PDMS layer corresponds.The utility model can be used for single cell analysis, and chip takes into account two kinds of material advantages of PDMS polymer and filter paper fibre.
Description
Technical field
The utility model relates to micro-fluidic chip design field, especially a kind of array for single cell analysis
The compound micro-fluidic chip of PDMS- paper base.
Background technique
Dimethyl silicone polymer (Polydimethylsiloxane, PDMS) has good optical property, thermal stability
And the advantages that bio-compatibility, it is one of main rapidoprint of micro-fluidic chip.Chip based on PDMS material, in cell
Manipulation, genes within cells detection of expression and immunofluorescence analysis etc. successful application.But the fluid stream in the microchannel PDMS
Dynamic to usually require to rely on external syringe pump or starting micro-valve structure control, there is still a need for complete in the lab for analysis test.
In the past 10 years, the chip processed using filter paper as basis material, i.e. paper substrate micro-fluidic chip gradually become low cost,
Portable and environment-friendly type biochemistry detection tool.By intrinsic capillary fiber structure inside filter paper, in paper substrate micro-fluidic chip
Fluid driving and control are realized by capillarity, eliminate dependence of the conventional microfluidic control to equipment such as precise injection pumps, especially suitable
Biochemical sample detection and analysis should be carried out under the conditions of resource constraint.But because filter paper fibre structure is mixed and disorderly point usually random
Cloth, this structure are difficult to realize the precise manipulation of the low abundance sample (such as unicellular) of trace.
Summary of the invention
In view of this, the purpose of the utility model is to provide a kind of array PDMS- paper bases for single cell analysis to answer
Micro-fluidic chip is closed, can be used for single cell analysis, chip takes into account two kinds of material advantages of PDMS polymer and filter paper fibre.
The utility model is realized using following scheme: a kind of array PDMS- paper base for single cell analysis is compound micro-
Fluidic chip is disposed with PDMS layer, glue-line, paper substrate and heat patch from top to bottom；
The capture array being made of more than one concave structure is provided in the PDMS layer；
The paper substrate includes the array being made of the hydrophilic matrix unit including hydrophilic paper base structure and hydrophobic structure；
Each concave structure each of in the paper substrate in hydrophilic matrix unit and the PDMS layer corresponds.
Further, including the glue-line is low melting-point agarose.
Further, the fusing point of the low melting-point agarose is 65 DEG C.
Further, the material of the hydrophobic structure is photosensitive resin.
Further, the compound micro-fluidic chip periphery of the array PDMS- paper base for single cell analysis is using pressure
The encapsulation of power film.
The control method of the compound micro-fluidic chip of array PDMS- paper base for single cell analysis, specifically: when
When cell current-carrying flows through the concave structure of PDMS layer, the cell of special diameter size will be detained in this configuration；The PDMS layer
The glue-line being made up of with paper substrate low melting-point agarose bonds, and is arranged in described after the heat patch heating of chip bottom
Glue-line melts, and falls into hydrophilic paper base structure by cold and hot convection current cell；When cell pyrolysis liquid flows through hydrophilic paper base structure, carefully
The cell wall chemical cracking of born of the same parents discharges the substance including RNA, DNA, and DNA molecular chain is extracted by the micro- magnetic bead of solid phase, and is passed through
Polymerase chain reaction cyclic amplification, polymerase chain reaction product signal are directly read by fluorescence microscope.
Compared with prior art, the utility model has the following beneficial effects: the chip of the utility model to take into account PDMS poly-
Two kinds of material advantages of object and filter paper fibre are closed, integrating cell captures, is fixed, and DNA extraction and PCR cycle amplification etc. are multi-functional, saves
A variety of instrument and equipments required for previous cell experiment have been removed, analysis experiment flow is greatlied simplify.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model embodiment, wherein (a) is that schematic diagram is cutd open in side, it (b) is corresponding crosscutting
Schematic diagram.
Fig. 2 is the chip manufacturing flow diagram of the embodiment of the present invention.
Fig. 3 is the chip operation flow diagram of the embodiment of the present invention.
Specific embodiment
The utility model is described further with reference to the accompanying drawings and embodiments.
As shown in Figure 1, it is compound micro-fluidic to present embodiments provide a kind of array PDMS- paper base for single cell analysis
Chip is disposed with PDMS layer, glue-line, paper substrate and heat patch from top to bottom；
The capture array being made of more than one concave structure is provided in the PDMS layer；
The paper substrate includes the array being made of the hydrophilic matrix unit including hydrophilic paper base structure and hydrophobic structure；
Each concave structure each of in the paper substrate in hydrophilic matrix unit and the PDMS layer corresponds.
In the present embodiment, including the glue-line is low melting-point agarose.
In the present embodiment, the fusing point of the low melting-point agarose is 65 DEG C.
In the present embodiment, the material of the hydrophobic structure is photosensitive resin.
In the present embodiment, the compound micro-fluidic chip periphery of array PDMS- paper base for single cell analysis is adopted
It is encapsulated with pressure membrane.
The present embodiment is additionally provided based on the compound miniflow of array PDMS- paper base described above for single cell analysis
The control method of chip is controlled, specifically:
When cell current-carrying flows through the concave structure of PDMS layer, the cell of special diameter size will be detained in this configuration；
The PDMS layer and paper substrate are bonded by the glue-line that low melting-point agarose is constituted, and are arranged in described in chip bottom
The glue-line melts after heat patch heating, falls into hydrophilic paper base structure by cold and hot convection current cell；
When cell pyrolysis liquid flows through hydrophilic paper base structure, the cell wall chemical cracking release of cell includes that RNA, DNA exist
Interior substance, DNA molecular chain are extracted by the micro- magnetic bead of solid phase, and pass through polymerase chain reaction cyclic amplification, polymerase chain reaction
Product signal is answered to be directly read by fluorescence microscope.
Preferably, the chip of the present embodiment design uses function Integration Design, there is unicellular capture, release, fixed point
The functions such as cracking, targeting DNA fragmentation amplification.The part PDMS is used for unicellular capture, using " recessed " shape three-dimensional structure, when cell carries
When stream flows through the structure, the cell of special diameter size will be detained in this configuration；To avoid flow dead zone and irregular convection,
" recessed " shape three-dimensional structure upper dimension is wide as shown in Figure 1 with bottom.The hydrophobic structure of paper base part is constructed by photosensitive resin, is passed through
The processing of Conventional UV photoetching process；Hydrophilic-structure after processing is in array distribution, each unit and PDMS " recessed " shape three-dimensional structure
It corresponds.It is bonded with paper base part by low melting-point agarose in the part PDMS, agar melts after heating, by cold and hot convection current
Cell is fallen into hydrophilic paper base.When cell pyrolysis liquid flows through paper base unit, cell wall chemical cracking discharges RNA, DNA etc..DNA points
Subchain is extracted by the micro- magnetic bead of solid phase, and passes through polymerase chain reaction (PCR) cyclic amplification.In chip bottom, temperature control patch is set
(heat patch) to real-time control and monitors temperature in each reaction member.To avoid the sample being likely to occur in reaction evaporation,
Chip periphery is encapsulated using pressure membrane.Because PDMS translucency is preferable, PCR reaction product signal can directly be read by fluorescence microscope
It takes.Entire cell analysis process, which does not need the equipment such as syringe pump, can be completed.The present embodiment be put forward for the first time for Manipulation of single cells,
The compound microfluidic methods of array PDMS- paper base and feasible chip Prototype Design of intracellular DNA analysis, have multifunctional unit
The advantages that change, has a good application prospect in terms of portable analysis, Personalized medicine and trace sample.
Fig. 1 is the compound microfluidic chip structure schematic diagram of the present embodiment array PDMS- paper base.It is set in the cavity of the part PDMS
" recessed " shape three-dimensional matrix structure is set, Cell capture and fixation are used for；Low melting-point agarose is for being isolated PDMS and filter paper part；Filter
Paper part is made of hydrophilic rectangular element array, and each unit is corresponding with PDMS " recessed " shape three-dimensional structure, is used for intracellular DNA temperature
Control amplified reaction.
Fig. 2 is the micro-fluidic chip PDMS structure schematic diagram of the process of the present embodiment, the main base of the processing of PDMS structure
In standard soft lithography process.To obtain not wide " recessed " shape three-dimensional structure, using secondary photoetching process, narrow base section is hollow knot
Structure is used to support top capturing structure and avoids flow dead zone to greatest extent.Fusing point is used to do bottom envelope for 65 DEG C of agarose
Mouthful, PDMS is connected with paper base segment fluid flow/and closure realized by temperature control.
Fig. 3 is the micro-fluidic operational flowchart of PDMS- paper base compound chip of the present embodiment.Multiple steps can be compound in monolithic
It is completed on chip.To be easier to the reagent evaporation problems occurred when avoiding high temperature (95 DEG C), the present embodiment uses ring mediated isothermal side
Method (loop-mediated isothermal amplification) expands DNA sample, and this method reaction temperature is set as
65℃。
It is noted that the utility model protection is hardware configuration, it is not claimed as control method.Above only
For a preferable embodiment in the utility model embodiment.But the utility model is not limited to the embodiment above, it is all
Any equivalent change and modification done by the utility model, range of the generated function without departing from this programme
When, belong to the protection scope of the utility model.
Claims (5)
1. a kind of compound micro-fluidic chip of array PDMS- paper base for single cell analysis, it is characterised in that: from top to bottom according to
It is secondary to be provided with PDMS layer, glue-line, paper substrate and heat patch；
The capture array being made of more than one concave structure is provided in the PDMS layer；
The paper substrate includes the array being made of the hydrophilic matrix unit including hydrophilic paper base structure and hydrophobic structure；
Each concave structure each of in the paper substrate in hydrophilic matrix unit and the PDMS layer corresponds.
2. a kind of compound micro-fluidic chip of array PDMS- paper base for single cell analysis according to claim 1,
It is characterized in that: being low melting-point agarose including the glue-line.
3. a kind of compound micro-fluidic chip of array PDMS- paper base for single cell analysis according to claim 2,
Be characterized in that: the fusing point of the low melting-point agarose is 65 DEG C.
4. a kind of compound micro-fluidic chip of array PDMS- paper base for single cell analysis according to claim 1,
Be characterized in that: the material of the hydrophobic structure is photosensitive resin.
5. a kind of compound micro-fluidic chip of array PDMS- paper base for single cell analysis according to claim 1,
Be characterized in that: the compound micro-fluidic chip periphery of array PDMS- paper base for single cell analysis is encapsulated using pressure membrane.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109289954A (en) * | 2018-11-28 | 2019-02-01 | 福州大学 | A kind of compound micro-fluidic chip of array PDMS- paper base and its control method for single cell analysis |
| CN110951605A (en) * | 2020-02-10 | 2020-04-03 | 福州大学 | Array type paper-based chip capable of being used for 2019-nCoV virus high-throughput detection and manufacturing method thereof |
| CN111879922A (en) * | 2020-07-13 | 2020-11-03 | 东南大学 | Integrated paper-based chip structure suitable for synchronous detection of nucleic acid and immunity and manufacturing method thereof |
| WO2021133318A1 (en) * | 2019-12-27 | 2021-07-01 | T.C. Erci̇yes Üni̇versi̇tesi̇ | Preparation method of a base suitable for use to improve the durability of the superhydrophobic surfaces |
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2018
- 2018-11-28 CN CN201822017124.7U patent/CN209222159U/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109289954A (en) * | 2018-11-28 | 2019-02-01 | 福州大学 | A kind of compound micro-fluidic chip of array PDMS- paper base and its control method for single cell analysis |
| CN109289954B (en) * | 2018-11-28 | 2024-04-09 | 福州大学 | Array PDMS-paper-based composite microfluidic chip for single cell analysis and control method thereof |
| WO2021133318A1 (en) * | 2019-12-27 | 2021-07-01 | T.C. Erci̇yes Üni̇versi̇tesi̇ | Preparation method of a base suitable for use to improve the durability of the superhydrophobic surfaces |
| CN110951605A (en) * | 2020-02-10 | 2020-04-03 | 福州大学 | Array type paper-based chip capable of being used for 2019-nCoV virus high-throughput detection and manufacturing method thereof |
| CN110951605B (en) * | 2020-02-10 | 2020-10-09 | 福州大学 | Array type paper-based chip capable of being used for 2019-nCoV virus high-throughput detection and manufacturing method thereof |
| WO2021159867A1 (en) * | 2020-02-10 | 2021-08-19 | 福州大学 | Array-type paper-based chip capable of being used for high-throughput testing of 2019-ncov virus, and manufacturing method therefor |
| CN111879922A (en) * | 2020-07-13 | 2020-11-03 | 东南大学 | Integrated paper-based chip structure suitable for synchronous detection of nucleic acid and immunity and manufacturing method thereof |
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