CN201817488U - Microcentrifuge tube - Google Patents
Microcentrifuge tube Download PDFInfo
- Publication number
- CN201817488U CN201817488U CN2010205701217U CN201020570121U CN201817488U CN 201817488 U CN201817488 U CN 201817488U CN 2010205701217 U CN2010205701217 U CN 2010205701217U CN 201020570121 U CN201020570121 U CN 201020570121U CN 201817488 U CN201817488 U CN 201817488U
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- CN
- China
- Prior art keywords
- tube
- lid
- groove
- bassoon
- tube chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Abstract
The utility model relates to a microcentrifuge tube. The microcentrifuge tube comprises a tube body and a large tube cap connected with the tube body, and further comprises a tube cavity, a small tube cap and a groove with an extending periphery; the tube cavity is formed on the large tube cap; the upper end of the tube cavity is connected with the small tube cap; the lower end of the tube cavity is communicated with the large tube cap; a bulge is formed on the lower surface of the large tube cap; the extending periphery of the groove is fixedly clamped on the lower surface of the large tube cap through the bulge; the groove is positioned under the tube cavity; and the perimeter of the groove is larger than or equal to the perimeter of the tube cavity. The microcentrifuge tube can prevent the environment of a laboratory from being polluted by the nucleic acid amplification product.
Description
Technical field
The utility model relates to a kind of Biochemistry Experiment consumptive material, relates in particular to the Eppendorf tube that a kind of nucleic acid amplification reaction uses.
Background technology
Nucleic acid amplification technologies be a kind of with a small amount of biotinylated nucleic acid sample as substrate, the enzyme catalysis system by specific makes it the technology at amplification in vitro, comprises polymerase chain reaction (PCR) amplification (PCR), relies on the constant-temperature amplification (HDA) of helicase etc.With each component in the reaction system by appropriate proportioning add carry out amplified reaction (needing PCR instrument or Other Instruments to come control reaction temperature usually) in the centrifuge tube after, need uncap usually adds other reagent and carries out next step reaction, for example enzyme is cut, dyeing etc.Existing centrifuge tube mostly is the cavity pipe of taper slightly, also has a gag to link to each other with the effective frenulum of cavity.Because the copy number of nucleic acid can be expanded to more than 1,000,000 times usually, in actually operating, the process of uncapping very easily causes nucleic acid fragment to diffuse in the air, the reaction that contaminate subsequent character is close.For example, hospital is when detecting pathogenic agent with molecule diagnosis kit, and pollution of nucleic acid can cause false-positive diagnostic result.Therefore provide a kind of centrifuge tube that can prevent the nucleic acid amplification product contaminate environment, extremely important for the experimental result of long-term stability.
The utility model content
In view of this, the purpose of this utility model provides a kind of can open centrifuge tube in that nucleic acid amplification product is carried out subsequent reactions the time, prevents the Eppendorf tube of its contaminate environment.
Eppendorf tube described in the utility model, the bassoon lid 2 that comprises pipe shaft 1 and be attached thereto, also comprise tube chamber 21, tubule lid 22 and have the groove 3 that extends periphery, described bassoon lid 2 is provided with tube chamber 21, the upper end of described tube chamber 21 links to each other with tubule lid 22, lower end and bassoon lid 2 communicate, the lower surface of described bassoon lid 2 is provided with projection 4, the periphery that groove 3 extends fixes on the lower surface of bassoon lid 2 by projection 4, and groove 3 is positioned at the below of tube chamber 21, and its all footpath is more than or equal to the Zhou Jing of tube chamber 21.
Further, the Zhou Jing of described groove 3 equates with the week footpath of tube chamber 21.
Further, described pipe shaft (1) and bassoon lid (2) is made one and is connected frenulum; Described tube chamber (21) is made one with tubule lid (22) and is connected frenulum.
Eppendorf tube described in the utility model, be that pipe at existing centrifuge tube covers and increases by one again and be made up of tubule lid, tube chamber and groove, the micro tube that can independently add reagent, when in nucleic acid amplification product, adding subsequent reactions reagent, can directly open the tubule lid and add, and then the tubule of pushing on the micro tube covers, make the bulging deformation that fixes groove, groove is fallen in the pipe shaft of centrifuge tube, and follow-up reagent and nucleic acid amplification product mixing are reacted, and have prevented the pollution of nucleic acid environment simultaneously.The another kind of selection is before nucleic acid amplification reaction follow-up reagent to be added in advance, waits and pushes the tubule mixing when needing again.
Description of drawings
Accompanying drawing is used to provide further understanding of the present utility model, and constitutes the part of specification sheets, is used from explanation the utility model with embodiment one of the present utility model, does not constitute restriction of the present utility model.In the accompanying drawings:
Fig. 1 is the cross-sectional view of the utility model Eppendorf tube.
Embodiment
As shown in Figure 1, Eppendorf tube of the present utility model, the bassoon lid 2 that comprises pipe shaft 1 and be attached thereto, the lower tube of pipe shaft 1 is conical, pipe shaft 1 is made one with bassoon lid 2 and is connected frenulum, can when in pipe shaft 1, adding the PCR product, make bassoon lid 2 bands like this around pipe shaft 1, prevent that bassoon from covering 2 contaminated.Eppendorf tube of the present utility model also comprises tube chamber 21, tubule lid 22 and has the groove 3 that extends periphery, be inlaid with tube chamber 21 on the described bassoon lid 2, the upper end of described tube chamber 21 is made one with tubule lid 22 and is connected frenulum, lower end and bassoon lid 2 communicate, form a through hole, the lower surface of described bassoon lid 2 is provided with the projection 4 that section is the L type, is distributed in the periphery of through hole, and the periphery that groove 3 extends fixes on the minor face of projection 4; Groove 3 be positioned at tube chamber 21 under, its week footpath for equaling the Zhou Jing of tube chamber 21, just can make projection 4 be out of shape with less power more than or equal to the Zhou Jing of tube chamber 21 among this embodiment like this, thereby makes groove 3 disengagings.
Eppendorf tube of the present utility model is opened bassoon lid 2 earlier in use, adds each component of nucleic acid amplification reaction, and reaction is carried out; When finishing, open tubule lid 22 again, add secondary reaction reagent to groove 3 Deng amplified reaction; With defeating tubule lid 22, make with tubule lid 22 tube chambers that contact 21 and cover 2 one downward power then, make the projection 4 that bassoon is covered on 2 lower surfaces that deformation take place, break away from the groove 3 of its fixing, groove 3 is fallen in the pipe shaft 1, solution is mixed to bassoon.It is just to add secondary reaction reagent before nucleic acid amplification reaction in groove 3 that another kind is selected, and makes solution mixing up and down but push tubule lid 22 again when needs.
The above is a preferred embodiment of the present utility model only, is not limited to the utility model, and for a person skilled in the art, the utility model can have various changes and variation.All within spirit of the present utility model and principle, any modification of being done, be equal to replacement, improvement etc., all should be included within the protection domain of the present utility model.
Claims (4)
1. Eppendorf tube, the bassoon lid (2) that comprises pipe shaft (1) and be attached thereto, it is characterized in that: also comprise tube chamber (21), tubule lid (22) and groove (3) with extension periphery, described bassoon lid (2) is provided with tube chamber (21), the upper end of described tube chamber (21) links to each other with tubule lid (22), lower end and bassoon lid (2) communicate, the lower surface of described bassoon lid (2) is provided with projection (4), the periphery that groove (3) extends fixes on the lower surface of bassoon lid (2) by projection (4), and groove (3) is positioned at the below of tube chamber (21), and its all footpath is more than or equal to the Zhou Jing of tube chamber (21).
2. Eppendorf tube according to claim 1 is characterized in that: the Zhou Jing of described groove (3) equates with the week footpath of tube chamber (21).
3. Eppendorf tube according to claim 1 is characterized in that: described pipe shaft (1) is made one with bassoon lid (2) and is connected frenulum.
4. Eppendorf tube according to claim 1 is characterized in that: described tube chamber (21) is made one with tubule lid (22) and is connected frenulum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205701217U CN201817488U (en) | 2010-10-21 | 2010-10-21 | Microcentrifuge tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205701217U CN201817488U (en) | 2010-10-21 | 2010-10-21 | Microcentrifuge tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201817488U true CN201817488U (en) | 2011-05-04 |
Family
ID=43915451
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010205701217U Expired - Lifetime CN201817488U (en) | 2010-10-21 | 2010-10-21 | Microcentrifuge tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201817488U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111141899A (en) * | 2019-02-25 | 2020-05-12 | 上海快灵生物科技有限公司 | Biochemical reaction test paper strip tube and use method and kit thereof |
| CN111218392A (en) * | 2019-02-25 | 2020-06-02 | 上海快灵生物科技有限公司 | Biochemical reaction test tube, use method thereof and gene amplification kit |
-
2010
- 2010-10-21 CN CN2010205701217U patent/CN201817488U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111141899A (en) * | 2019-02-25 | 2020-05-12 | 上海快灵生物科技有限公司 | Biochemical reaction test paper strip tube and use method and kit thereof |
| CN111218392A (en) * | 2019-02-25 | 2020-06-02 | 上海快灵生物科技有限公司 | Biochemical reaction test tube, use method thereof and gene amplification kit |
| CN111218392B (en) * | 2019-02-25 | 2020-11-27 | 上海快灵生物科技有限公司 | Biochemical reaction test tube, use method thereof and gene amplification kit |
| CN111141899B (en) * | 2019-02-25 | 2021-05-18 | 上海快灵生物科技有限公司 | Biochemical reaction test paper strip tube and use method and kit thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20110504 |