CN209468427U - It is a kind of to mix the biochip reaction device operated suitable for high-intensitive - Google Patents
It is a kind of to mix the biochip reaction device operated suitable for high-intensitive Download PDFInfo
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- CN209468427U CN209468427U CN201822155706.1U CN201822155706U CN209468427U CN 209468427 U CN209468427 U CN 209468427U CN 201822155706 U CN201822155706 U CN 201822155706U CN 209468427 U CN209468427 U CN 209468427U
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- reaction
- biochip
- accommodating chamber
- plug connector
- pressing plate
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Abstract
The utility model provides a kind of suitable for the high-intensitive biochip reaction device for mixing operation, including the internal accommodating chamber for hollow design, plug connector is inserted in the accommodating chamber, the top of the plug connector is equipped with the pressing plate for support biochip, elastic component is equipped between the pressing plate and the top surface of the plug connector, the inner wall of the accommodating chamber towards at the position of the pressing plate circumferentially arranged with boss, the boss is circumferentially arranged with buffer layer and the buffer layer is arranged towards the pressing plate, the biochip is crimped between the buffer layer and the pressing plate, the boss and the buffer layer cavity made of constitute reaction chamber, the accommodating chamber, which corresponds to, is equipped with liquid injection hole and gas vent at the position of the reaction chamber, after the liquid injection hole is used to that reaction solution to be added into the reaction chamber and reaction solution is added, liquid injection hole is closed, it is described After gas vent is for being discharged the indoor air of the reaction and the discharge indoor air of reaction, gas vent is closed.The utility model can significantly absorb oscillating force, efficiently avoid the broken problem of biochip.
Description
Technical field
The utility model belongs to biochip technology field, and in particular to a kind of suitable for the high-intensitive biology for mixing operation
Chip reactor.
Background technique
Biochip is the new and high technology developed rapidly in life science in recent years, it is mainly
Refer to the chemistry of micro-organisms system by micro-processing technology in only several square centimeters of solid chip surface building, with realization pair
Quick, the accurate and high-throughput detection of the biological samples such as DNA, protein, cell.Its main testing principle are as follows: useization
Or physical method, a large amount of probes are solidified in the surface of support, then according to the affinity interaction between biomolecule, such as core
The base pairing effect of acid molecule, combination of antigen-antibody etc. are reacted, then are tested and analyzed to reaction signal, can be obtained
To the relevant information of the sample.Since a large amount of probe can be fixed on support by the technology simultaneously, only need primary
A large amount of biomolecule can be tested and analyzed, have the characteristics that high automation, high throughput.
Incubation step is one of the important link in biochip operation, during incubation, free targeting in liquid phase
Molecule or identification molecule pass through liquid-solid boundary barrier and the probe molecule of the surface of solids is mutually distinguishable and combines, the reaction of the process
Rate and efficiency are influenced by solutes accumulation.To improve reaction rate, frequently with the method for high-intensity oscillation incubation.Oscillation incubation
In the process, to prevent biochip mobile, need to compress it positioning, Chinese utility model patent CN202766517U is disclosed
A kind of biochip incubation reaction device, including reaction cavity, and the reaction column with the mating installation of reaction cavity；The reaction cavity
Inside top is provided with boss, and sealant is provided on the inside of the boss, and the sealant, boss and reaction cavity are integrally formed
And sealant has the hollow structure as incubation reaction chamber, is provided with and is used between the sealant and the upper surface of reaction column
The gap of biochip is installed, the note for filling incubation reaction liquid into incubation reaction chamber is provided at the top of the reaction cavity
Fluid apertures and venthole for air in incubation reaction chamber to be discharged, the liquid injection hole and venthole both pass through boss and incubation reaction
Chamber is connected.The utility model by being pushed into reaction column into reaction cavity, thus by biochip be pressed on reaction column with it is convex
Between platform, but it has two: (1) being rigid contact between biochip and reaction column, without any buffering, high-strength
It is easy to cause biochip broken in degree oscillatory process；(2) the reaction column push-in intracorporal distance of reaction chamber is fixed, Zhi Nengshi
For fixing the biochip of specification.
Utility model content
The utility model provides a kind of suitable for high-strength for the fixed form that biochip rigidly compresses in the prior art
Degree mixes the biochip reaction device of operation, can significantly absorb oscillating force, and it is broken to efficiently avoid biochip
Broken problem.
The utility model adopts the following technical solution:
A kind of is the accommodating chamber of hollow design including inside suitable for the high-intensitive biochip reaction device for mixing operation,
Plug connector is inserted in the accommodating chamber, the top of the plug connector is equipped with the pressing plate for support biochip, the pressing plate
It is equipped with elastic component between the top surface of the plug connector, the inner wall of the accommodating chamber is towards circumferentially setting at the position of the pressing plate
There is boss, the boss is circumferentially arranged with buffer layer and the buffer layer is arranged towards the pressing plate, the biochip crimping
Between the buffer layer and the pressing plate, the boss and the buffer layer cavity made of constitute reaction chamber, the receiving
Room, which corresponds to, is equipped with liquid injection hole and gas vent at the position of the reaction chamber, the liquid injection hole into the reaction chamber for being added
After reaction solution and addition reaction solution, liquid injection hole is closed, and the gas vent is for being discharged the indoor air of the reaction and discharge institute
After stating the indoor air of reaction, gas vent is closed.
Preferably, the plug connector is integrally inverted U shape, and the plug connector by top plate and is symmetrically set in top plate two sides
Side plate is constituted, and is connected between the both side plate by a crossbeam, and the top plate, both side plate and crossbeam are integrally formed.
Preferably, the two sides inner wall of the both side plate and accommodating chamber is in close contact.
Preferably, the outer wall of the both side plate is symmetrically arranged with convex block, and the two sides inner wall of the accommodating chamber is respectively equipped with and institute
The card slot that convex block matches is stated, the plug connector is inserted in the accommodating chamber by the cooperation of the convex block and the card slot.
Preferably, the convex block and the side plate are integrally formed, and the convex block and the side plate joint use circular arc
Transition Design.
Preferably, the elastic component is spring.
The beneficial effects of the utility model are as follows:
Biochip incubation reaction high-intensity oscillation mix operation in, biochip by biggish active force, and
The direction of active force is changeable, and biochip is mostly flake structure, and brittleness is big, to prevent biochip from mixing in high-intensity oscillation
It is crushed in operation, the utility model makes improvement to plug connector first, and the top of the plug connector, which is equipped with, is used for support biology core
The pressing plate of piece, being equipped with elastic component between the pressing plate and the top surface of the plug connector can be significantly due to the presence of elastic component
Ground absorbs oscillating force, efficiently avoids the broken problem of biochip, further, since the presence of elastic component, this is practical
The novel biochip that can be suitable for different size, only needs elastic component to be deformed according to the thickness elastomeric of biochip；Institute
The inner wall of accommodating chamber is stated towards circumferentially arranged with boss, the boss is circumferentially arranged with buffer layer and institute at the position of the pressing plate
Buffer layer to be stated to be arranged towards the pressing plate, buffer layer has further buffered the oscillating force acted on biochip,
One layer of protection more to biochip.
Detailed description of the invention
In order to illustrate the technical solutions in the embodiments of the present application or in the prior art more clearly, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
The some embodiments recorded in application, for those of ordinary skill in the art, without creative efforts,
It is also possible to obtain other drawings based on these drawings；
Fig. 1 is the structural schematic diagram of embodiment 1；
Fig. 2 is the schematic diagram of internal structure of accommodating chamber top plate in embodiment 1；
Fig. 3 is 1 use state diagram of embodiment；
1, accommodating chamber；2, liquid injection hole；3, gas vent；4, card slot；5, plug connector；6, convex block；7, buffer layer；8, washer；9,
Boss；10, spring；11, crossbeam；12, biochip；13, reaction chamber；14, the first rubber plug；15, the second rubber plug.
Specific embodiment
In order to keep the technical purpose, technical scheme and beneficial effects of the utility model clearer, with reference to the accompanying drawing and
Specific embodiment is further illustrated the technical solution of the utility model.
Embodiment 1
As illustrated in fig. 1 and 2, a kind of suitable for the high-intensitive biochip reaction device for mixing operation, including internal is hollow
The accommodating chamber 1 of design, the accommodating chamber 1 can be the rectangular-shape of open at one end, and in the present embodiment, the accommodating chamber 1 is in falling U
Shape, and the accommodating chamber 1 by the first top plate and is symmetrically set in the first side plate of the first top plate two sides and the second side plate is constituted, institute
It states and is inserted with plug connector 5 in accommodating chamber 1, the plug connector 5 is whole to be inverted U shape, and the plug connector 5 is by the second top plate and right
The third side plate for being set to the second top plate two sides and the 4th side plate is claimed to constitute, the third side plate and the 4th side plate and accommodating chamber 1
Two sides inner wall is in close contact.It is connected between the third side plate and the 4th side plate by a crossbeam 11, second top plate,
Third side plate, the 4th side plate and crossbeam 11 are integrally formed, the effect of the crossbeam 11 are as follows: are completed in reaction, needed to take out biology
When chip, it can be exerted a force by crossbeam 11 and extract plug connector 5.For the tightness of reinforcement structure, the third side plate and the 4th side plate
Outer wall be symmetrically arranged with convex block 6, the two sides inner wall of the accommodating chamber 1 is respectively equipped with the card slot 4 to match with the convex block 6, institute
Plug connector 5 is stated to be inserted in the accommodating chamber 1 by the cooperation of the convex block 6 and the card slot 4.The convex block 6 and described the
Three side plates or the 4th side plate are integrally formed, and the joint of the convex block 6 and the third side plate or the 4th side plate uses circular arc
Transition Design, to facilitate convex block 6 to slide in or out in card slot 4.
The top of the plug connector 5 is equipped with the pressing plate 7 for support biochip, the pressing plate 7 and the plug connector 5
Spring 10 is equipped between top surface, spring 10 is vertically arranged and the top of spring 10 and bottom end are fixed with pressing plate 7 and plug connector 5 respectively
Connection；When in use, spring 10 is in compressive state always, on the one hand can securely compress biochip, on the other hand may be used
The external forces being subject to organism-absorbing chip；The inner wall of first top plate towards at the position of the pressing plate 7 circumferentially
Equipped with boss 9, the boss 9 is circumferentially arranged with buffer layer 8 and the buffer layer 8 is arranged towards the pressing plate 7, the biology core
Piece is crimped between the buffer layer 8 and the pressing plate 7, and the boss 9 circumferentially forms a rectangle, and (this is rectangular
Area coverage should be enough to place biochip), the buffer layer 8 also constitutes a rectangle along the circumferentially extending of boss 9；The boss
9 and the cavity made of of buffer layer 8 constitute reaction chamber 13, in the present embodiment, first top plate corresponds to the reaction chamber
Liquid injection hole 2 and gas vent 3 are equipped at 13 position, the liquid injection hole 2 is used to that reaction solution to be added into the reaction chamber 13 and adds
Using the first rubber plug 14 that liquid injection hole 2 is closed after entering reaction solution, the gas vent 3 is used for the sky being discharged in the reaction chamber 13
It is using the second rubber plug 15 that gas vent 3 is closed after air in gas and the discharge reaction chamber 13.
When the utility model is used, as described in Figure 3, first in the fixed biochip 12 in the top surface of pressing plate 7, biochip
12 wait react up, then hold crossbeam 11 plug connector 5 is inserted in accommodating chamber 1, when convex block 6 is caught in card slot 4,
It looses one's grip；Then reaction liquid is added into reaction chamber 13 by liquid injection hole 2, while reaction liquid is added, in reaction chamber 13
Air gradually passes through the discharge of gas vent 3, is finally the peace for completing the utility model using rubber plug sealing liquid injection hole 2 and gas vent 3
Dress transfers them in high-speed oscillator and carries out the incubation of biochip.
It should be noted last that: the technical solution of the utility model that the above embodiments are only illustrative and not limiting is appointed
What to the utility model carry out equivalent replacement and do not depart from the spirit and scope of the utility model modification or part replace,
It should cover within the scope of the utility model claims protection.
Claims (6)
1. it is a kind of suitable for the high-intensitive biochip reaction device for mixing operation, including the internal accommodating chamber for hollow design, institute
It states and is inserted with plug connector in accommodating chamber, it is characterised in that: the top of the plug connector is equipped with the pressing plate for support biochip,
It is equipped with elastic component between the pressing plate and the top surface of the plug connector, the inner wall of the accommodating chamber is towards at the position of the pressing plate
Circumferentially arranged with boss, the boss is circumferentially arranged with buffer layer and the buffer layer is arranged towards the pressing plate, the biology
Chip is crimped between the buffer layer and the pressing plate, and the boss and the buffer layer cavity made of constitute reaction chamber,
The accommodating chamber, which corresponds to, is equipped with liquid injection hole and gas vent at the position of the reaction chamber, the liquid injection hole is used for the reaction
After indoor addition reaction solution and addition reaction solution, liquid injection hole is closed, and the gas vent is for being discharged the indoor air of reaction
And after the discharge indoor air of reaction, gas vent is closed.
2. according to claim 1 be suitable for the high-intensitive biochip reaction device for mixing operation, it is characterised in that: described
Plug connector is integrally inverted U shape, and the plug connector by top plate and is symmetrically set in the side plates of top plate two sides and constitutes, the both side plate
Between be connected by a crossbeam, the top plate, both side plate and crossbeam are integrally formed.
3. according to claim 2 be suitable for the high-intensitive biochip reaction device for mixing operation, it is characterised in that: described
The two sides inner wall of both side plate and accommodating chamber is in close contact.
4. according to claim 3 be suitable for the high-intensitive biochip reaction device for mixing operation, it is characterised in that: described
The outer wall of both side plate is symmetrically arranged with convex block, and the two sides inner wall of the accommodating chamber is respectively equipped with the card slot to match with the convex block,
The plug connector is inserted in the accommodating chamber by the cooperation of the convex block and the card slot.
5. according to claim 4 be suitable for the high-intensitive biochip reaction device for mixing operation, it is characterised in that: described
Convex block and the side plate are integrally formed, and the convex block and the side plate joint are designed using arc transition.
6. according to claim 1 be suitable for the high-intensitive biochip reaction device for mixing operation, it is characterised in that: described
Elastic component is spring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822155706.1U CN209468427U (en) | 2018-12-21 | 2018-12-21 | It is a kind of to mix the biochip reaction device operated suitable for high-intensitive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822155706.1U CN209468427U (en) | 2018-12-21 | 2018-12-21 | It is a kind of to mix the biochip reaction device operated suitable for high-intensitive |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209468427U true CN209468427U (en) | 2019-10-08 |
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ID=68089200
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201822155706.1U Active CN209468427U (en) | 2018-12-21 | 2018-12-21 | It is a kind of to mix the biochip reaction device operated suitable for high-intensitive |
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| Country | Link |
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| CN (1) | CN209468427U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111197459A (en) * | 2020-02-28 | 2020-05-26 | 贵州捷盛钻具股份有限公司 | Drilling tool clamp |
-
2018
- 2018-12-21 CN CN201822155706.1U patent/CN209468427U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111197459A (en) * | 2020-02-28 | 2020-05-26 | 贵州捷盛钻具股份有限公司 | Drilling tool clamp |
| CN111197459B (en) * | 2020-02-28 | 2021-04-23 | 贵州捷盛钻具股份有限公司 | Drilling tool clamp |
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