CN105232831A - Application of extract mixture of Acorus gramineus Soland., nutmeg and clove to treatment of cerebral ischemia - Google Patents
Application of extract mixture of Acorus gramineus Soland., nutmeg and clove to treatment of cerebral ischemia Download PDFInfo
- Publication number
- CN105232831A CN105232831A CN201510791816.5A CN201510791816A CN105232831A CN 105232831 A CN105232831 A CN 105232831A CN 201510791816 A CN201510791816 A CN 201510791816A CN 105232831 A CN105232831 A CN 105232831A
- Authority
- CN
- China
- Prior art keywords
- volatile oil
- rhizoma acori
- acori graminei
- flos caryophylli
- semen myristicae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an extraction method of an extract mixture of Acorus gramineus Soland., nutmeg and clove, which comprises the steps of refluxing Acorus gramineus Soland., nutmeg and clove mixture to obtain Acorus gramineus Soland., nutmeg and clove mixed volatile oil, filtrate and medicine dregs; and further treating the Acorus gramineus Soland., nutmeg and clove mixed volatile oil, filtrate and medicine dregs to obtain the extract mixture of Acorus gramineus Soland., nutmeg and clove. Compared with the prior art, a composition with mass fraction of 2% prepared by mixing the extract mixture of Acorus gramineus Soland., nutmeg and clove with distilled water is capable of preventing and treating ischemic cerebrovascular diseases, and has promotion use values, and the problems that the existing Western medicines for treating cerebral ischemia have single medicine action targets, and the traditional Chinese medicine compound preparation for treating cerebral ischemia is large in prescription, many in medicines, large in taking amount and inconvenient to use in the prior art are solved.
Description
Technical field
The invention belongs to biomedicine technical field, particularly relate to the extracting method that a kind of Rhizoma Acori Graminei, Semen Myristicae and Flos Caryophylli extract mixture.
Background technology
Cerebral ischemia is one of principal disease posing a health risk at present and survive, also be the common disease of middle-aged and elderly people, the feature of this disease is that sickness rate is high, mortality rate is high, disability rate is high, relapse rate is high, having a strong impact on life expectancy and the quality of life of patient, is a kind of disease of current emphasis prevention and therapy.At present, the medicine of clinical treatment cerebral ischemia has dehydrant, diuretic, vasodilator, calcium antagonist, improves brain metabolism medicine, anticoagulation, Thrombolytic Drugs etc., but effect is unsatisfactory, have also with serious adverse reaction.Therefore, treatment ischemic cerebrovascular medicine is significant safely and effectively in positive searching.Especially, in recent years, under the world trends of " back to nature ", from Chinese medicine, the focus that thinking and medicine source become research is found.
Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli are all containing volatile oil.Large quantity research confirms, volatile oil has the effects such as antiinflammatory, antiallergic, antimicrobial, mutation and anticancer, anthelmintic.
Up to now, there is not yet the report of Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli extraction mixture treatment ischemic cerebrovascular.
Summary of the invention
Object of the present invention is just to provide a kind of Rhizoma Acori Graminei, Semen Myristicae and Flos Caryophylli to extract the extracting method of mixture to solve the problem.
The present invention is achieved through the following technical solutions above-mentioned purpose:
The present invention includes following steps:
Step 1: refluxed by the mixture of Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli, prepares Rhizoma Acori Graminei, Semen Myristicae, the Mixed chaotic sequences of Flos Caryophylli, filtrate and medicinal residues, and volatile oil and beta-schardinger dextrin-is carried out enclose, obtain the Benexate Hydrochloride of volatile oil;
Step 2, the medical material slag in step 1 is added 10 times of water gaging heating and refluxing extraction 1.5 hours again, squeeze clean medicinal liquid, filtered while hot; Filtrate filtrate and step 1 obtained merges, hold over night under not higher than 15 DEG C of environment, and after collecting the floating yellow solid oils and fats of solution, remaining solution Rotary Evaporators concentrates evaporate to dryness, obtains dry extract for subsequent use, for subsequent use after yellow solid oils and fats dehydrate;
Step 3, medicinal residues remaining in step 2 95% alcohol heating reflux of 5 times amount is extracted 2 times, extraction time is respectively 6h, 4h, heat filtering, merging filtrate, 55 DEG C-75 DEG C decompression distilleds, and the oven dry of 50-60 DEG C, concentrated solution baking oven, obtains dry extract for subsequent use;
The dry extract prepared in step 4, dry extract step 2 obtained and dried oils and fats and step 3 is all ground into fine powder, and the Benexate Hydrochloride of the volatile oil obtained with step 1 mixes, and obtains Rhizoma Acori Graminei, Semen Myristicae and Flos Caryophylli and extracts mixture.
Further, in described step 1, the mixture of Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli is refluxed, prepares Rhizoma Acori Graminei, Semen Myristicae, the Mixed chaotic sequences of Flos Caryophylli, filtrate and medicinal residues and be specially:
Step 1.1, Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli three kinds of processing of crude drugs are become fragment, be that the ratio of 2-4:1-3:1 mixes with mass ratio, add the water of medical material volume about 10 times amount, reflux, extract, in multifunctional extracting pot also collects volatile oil; After anhydrous sodium sulfate dehydration, obtain the Mixed chaotic sequences of Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli three kinds of medical materials, for subsequent use;
When step 1.2, the volatilization oil mass distillated that continues to be back to no longer increase, continue reflux, extract, 2.0 hours, leach filtrate while hot, separately put, medicinal residues are separately put;
Step 1.3, the volatile oil raised is adopted polishing beta-cyclodextrin inclusion compound: the anhydrous alcohol solution volatile oil solution being made into volume fraction 50% with anhydrous alcohol solution volatile oil, get beta-schardinger dextrin-, add after 4 times amount distilled water grind well, slowly add the anhydrous alcohol solution volatile oil solution of volume fraction 50%, hand lapping 3h is to becoming pasty state, sucking filtration, clathrate petroleum ether rinses 3 times, each 10ml, 40 DEG C of vacuum drying 3-8h, the Benexate Hydrochloride of volatile oil must be done, for subsequent use.
Particularly, the particle diameter of described Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli three kinds of medical materials is 0.5cm.
Particularly, the mass volume ratio (g/ml) of the beta-schardinger dextrin-in described step 1.3 and volatile oil is 4:1-8:1.
Beneficial effect of the present invention is:
The present invention is the extracting method that a kind of Rhizoma Acori Graminei, Semen Myristicae and Flos Caryophylli extract mixture, compared with prior art, the Rhizoma Acori Graminei that method of the present invention prepares, Semen Myristicae and Flos Caryophylli are extracted mixture and mix with distilled water, be made into the compositions that mass fraction is 2%, ischemic cerebrovascular can be prevented and treated, the drug target solving the western medicine cerebral ischemia existed in prior art is single, the prescription of compound Chinese medicinal preparation treatment cerebral ischemia is too large, medicament categories is too many, dosage is too large, use inconvenient problem, has the value promoted the use of.
Detailed description of the invention
The invention will be further described below:
The present invention includes following steps:
Step 1: refluxed by the mixture of Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli, prepares Rhizoma Acori Graminei, Semen Myristicae, the Mixed chaotic sequences of Flos Caryophylli, filtrate and medicinal residues, and volatile oil and beta-schardinger dextrin-is carried out enclose, obtain the Benexate Hydrochloride of volatile oil;
Step 2, the medical material slag in step 1 is added 10 times of water gaging heating and refluxing extraction 1.5 hours again, squeeze clean medicinal liquid, filtered while hot; Filtrate filtrate and step 1 obtained merges, hold over night under not higher than 15 DEG C of environment, and after collecting the floating yellow solid oils and fats of solution, remaining solution Rotary Evaporators concentrates evaporate to dryness, obtains dry extract for subsequent use, for subsequent use after yellow solid oils and fats dehydrate;
Step 3, medicinal residues remaining in step 2 95% alcohol heating reflux of 5 times amount is extracted 2 times, extraction time is respectively 6h, 4h, heat filtering, merging filtrate, 55 DEG C-75 DEG C decompression distilleds, and the oven dry of 50-60 DEG C, concentrated solution baking oven, obtains dry extract for subsequent use;
The dry extract prepared in step 4, dry extract step 2 obtained and dried oils and fats and step 3 is all ground into fine powder, and the Benexate Hydrochloride of the volatile oil obtained with step 1 mixes, and obtains Rhizoma Acori Graminei, Semen Myristicae and Flos Caryophylli and extracts mixture.
Further, in described step 1, the mixture of Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli is refluxed, prepares Rhizoma Acori Graminei, Semen Myristicae, the Mixed chaotic sequences of Flos Caryophylli, filtrate and medicinal residues and be specially:
Step 1.1, Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli three kinds of processing of crude drugs are become fragment, be that the ratio of 2-4:1-3:1 mixes with mass ratio, add the water of medical material volume about 10 times amount, reflux, extract, in multifunctional extracting pot also collects volatile oil; After anhydrous sodium sulfate dehydration, obtain the Mixed chaotic sequences of Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli three kinds of medical materials, for subsequent use;
When step 1.2, the volatilization oil mass distillated that continues to be back to no longer increase, continue reflux, extract, 2.0 hours, leach filtrate while hot, separately put, medicinal residues are separately put;
Step 1.3, the volatile oil raised is adopted polishing beta-cyclodextrin inclusion compound: the anhydrous alcohol solution volatile oil solution being made into volume fraction 50% with anhydrous alcohol solution volatile oil, get beta-schardinger dextrin-, add after 4 times amount distilled water grind well, slowly add the anhydrous alcohol solution volatile oil solution of volume fraction 50%, hand lapping 3h is to becoming pasty state, sucking filtration, clathrate petroleum ether rinses 3 times, each 10ml, 40 DEG C of vacuum drying 3-8h, the Benexate Hydrochloride of volatile oil must be done, for subsequent use.
Particularly, the particle diameter of described Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli three kinds of medical materials is 0.5cm.
Particularly, the mass volume ratio (g/ml) of the beta-schardinger dextrin-in described step 1.3 and volatile oil is 4:1-8:1.
Embodiment 1
Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli mix with the ratio of 3:2:1, add 10 times amount water soakings after pulverizing, and extract volatile oil, by for subsequent use for the volatile oil beta-cyclodextrin inclusion compound raised by version pharmacopeia annex XD essential oil extraction method in 2005; Heat filtering after remainder decocting extracts, hold over night, after collecting the floating yellow solid oils and fats of solution, filtrate concentrates evaporate to dryness with Rotary Evaporators, obtains dry extract for subsequent use; Medicinal residues 95% alcohol heating reflux of 5 times amount extracts 2 times, and extraction time is respectively 6h, 4h, heat filtering, merging filtrate 65 DEG C decompression distilled, and the oven dry of 60 DEG C, concentrated solution baking oven, obtains dry extract for subsequent use; Above-mentioned dry extract and dried oil powder are broken into fine powder, mix with the β-cyclodextrin clathrate of volatile oil, obtain Rhizoma Acori Graminei, Semen Myristicae and Flos Caryophylli and extract mixture.
Embodiment 2
A kind of Rhizoma Acori Graminei, Semen Myristicae and Flos Caryophylli extract the preparation method of mixture, comprise the following steps: Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli three kinds of processing of crude drugs become particle diameter to be the fragment of 0.5cm, be that the ratio of 2:3:1 mixes with mass ratio, add the water of medical material volume about 10 times amount, reflux, extract, in multifunctional extracting pot also collects volatile oil; After anhydrous sodium sulfate dehydration, obtain the Mixed chaotic sequences of Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli three kinds of medical materials, for subsequent use; Continue to be back to the volatilization oil mass distillated when no longer increasing, continue reflux, extract, 2.0 hours, leach filtrate while hot, separately put, medicinal residues are separately put; Be made into the anhydrous alcohol solution volatile oil solution of volume fraction 50% with anhydrous alcohol solution volatile oil, get beta-schardinger dextrin-, add after 4 times amount distilled water grind well, slowly add the anhydrous alcohol solution volatile oil solution of volume fraction 50%, hand lapping 3h to becoming pasty state, sucking filtration, clathrate petroleum ether rinses 3 times, each 10ml, 40 DEG C of vacuum drying 8h, obtain the Benexate Hydrochloride of dry volatile oil, for subsequent use, wherein, the mass volume ratio (g/ml) of beta-schardinger dextrin-and volatile oil is 4:1.Above-mentioned medical material slag is added 10 times of water gaging heating and refluxing extraction 1.5 hours again, squeezes clean medicinal liquid, filtered while hot; Filtrate and above-mentioned filtrate are merged, hold over night under not higher than 15 DEG C of environment, after collecting the floating yellow solid oils and fats of solution, remaining solution Rotary Evaporators concentrates evaporate to dryness, obtains dry extract for subsequent use; For subsequent use after yellow solid oils and fats dehydrate; Above-mentioned remaining medicinal residues 95% alcohol heating reflux of 5 times amount is extracted 2 times, and extraction time is respectively 6h, 4h, heat filtering, merging filtrate, 75 DEG C of decompression distilleds, and the oven dry of 60 DEG C, concentrated solution baking oven, obtains dry extract for subsequent use; Dry extract obtained above and dried oils and fats carry out being ground into fine powder, mix with the Benexate Hydrochloride of above-mentioned volatile oil, obtain Rhizoma Acori Graminei, Semen Myristicae and Flos Caryophylli and extract mixture.
Embodiment 3
A kind of Rhizoma Acori Graminei, Semen Myristicae and Flos Caryophylli extract the preparation method of mixture, comprise the following steps: Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli three kinds of processing of crude drugs become particle diameter to be the fragment of 0.5cm, be that the ratio of 4:1:1 mixes with mass ratio, add the water of medical material volume about 10 times amount, reflux, extract, in multifunctional extracting pot also collects volatile oil; After anhydrous sodium sulfate dehydration, obtain the Mixed chaotic sequences of Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli three kinds of medical materials, for subsequent use; Continue to be back to the volatilization oil mass distillated when no longer increasing, continue reflux, extract, 2.0 hours, leach filtrate while hot, separately put, medicinal residues are separately put; The volatile oil raised is adopted polishing beta-cyclodextrin inclusion compound: the anhydrous alcohol solution volatile oil solution being made into volume fraction 50% with anhydrous alcohol solution volatile oil, get beta-schardinger dextrin-, add after 4 times amount distilled water grind well, slowly add the anhydrous alcohol solution volatile oil solution of volume fraction 50%, hand lapping 3h is to becoming pasty state, sucking filtration, clathrate petroleum ether rinses 3 times, each 10ml, 40 DEG C of vacuum drying 8h, obtain the Benexate Hydrochloride of dry volatile oil, for subsequent use, wherein, the mass volume ratio (g/ml) of beta-schardinger dextrin-and volatile oil is 8:1.Above-mentioned medical material slag is added 10 times of water gaging heating and refluxing extraction 1.5 hours again, squeezes clean medicinal liquid, filtered while hot; Filtrate and above-mentioned filtrate are merged, hold over night under not higher than 15 DEG C of environment, after collecting the floating yellow solid oils and fats of solution, remaining solution Rotary Evaporators concentrates evaporate to dryness, obtains dry extract for subsequent use; For subsequent use after yellow solid oils and fats dehydrate; Above-mentioned remaining medicinal residues 95% alcohol heating reflux of 5 times amount is extracted 2 times, and extraction time is respectively 6h, 4h, heat filtering, merging filtrate, 55 DEG C of decompression distilleds, and the oven dry of 50 DEG C, concentrated solution baking oven, obtains dry extract for subsequent use; Dry extract obtained above and dried oils and fats carry out being ground into fine powder, mix with the Benexate Hydrochloride of above-mentioned volatile oil, obtain Rhizoma Acori Graminei, Semen Myristicae and Flos Caryophylli and extract mixture.
Embodiment 4
The Rhizoma Acori Graminei prepared by embodiment 1, Semen Myristicae and Flos Caryophylli extract mixture and mix with distilled water and be made into the medicine that mass fraction is the control ischemic cerebrovascular of 2%.
Embodiment 5 Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli extract the zoopery of mixture treatment ischemic cerebrovascular
1 animal SD rat (cleaning grade), male, 50, body weight 200 ± 50g, tieing up tonneau China Experimental Animal Center by Beijing provides, credit number: SCXK (capital) 2010-0002.
2 methods
Rat is divided into sham operated rats, model group, by the heavy dose of group (0.4g/kg) of reagent, by dosage group (0.2g/kg) in reagent, by reagent small dose group (0.1g/kg) by 2.1 groupings and administration at random.Face medicine is made into desired concn by used time suspension with distilled water, respectively group rat is all with the capacity gastric infusion of 2ml/100g, 1 time/d, successive administration 30d.Sham operated rats and model group give the normal saline of same volume.
2.2 model preparations adopt bolt collimation method to make focal rat cerebral ischemia model.By diameter 0.24mm, 0.26mm and 0.28mm, the fishing line of long 60mm dips in the blunt bolt the end of a thread of nial polish one-tenth circle, and with marker pen 20mm place labelling above bolt the end of a thread, the bolt line made is immersed in heparin stand-by.Under room temperature (22 DEG C) condition, after 3% pentobarbital sodium 30mg/kg intraperitoneal injection of anesthesia rat, lie on the back on operating-table, fixing.Neck medisection skin, be separated left common carotid (CCA), external carotid artery (ECA), internal carotid artery (ICA), ligation ECA, CCA proximal part, to divide and an osculum is cut at place at the nearly tremulous pulse of CCA, mention ECA, CCA to stop blooding, slowly insert bolt line, until there is resistance not insert again to ICA, pumpback is a little, and bolt line insertion depth is (20 ± 2) mm (counting from CCA crotch).Ligation ICA, skin suture.Sham operated rats only makes incision of skin and blood vessel exfoliation, does not do the process of bolt line, and all the other operations are with other group.Postoperative rat insulation, observes indices.
2.3 index observing
2.3.1 Neuroscore observes the degree of animal behavior obstacle after animal regains consciousness.Take double-blind method to mark, the method for reference Longa etc. is to behavior disorder rank scores: impassivity afunction symptom, and movable normal person is 0 point; Can not full extension offside fore paw person it be 1 point; When creeping, appearance is 2 points to the offside person of turn-taking; During walking, health is 3 points to the offside person of toppling over; Can not walk voluntarily, loss of consciousness person is 4 points.The successful mark of model is that homonymy occurs that Homer levies (i.e. homonymy enophthalmos) and the scoring >1 person of dividing is thromboembolism success.Otherwise it is disallowable.
2.3.2 cerebral infarction assessment of indices is after rat cerebral ischemia model sets up 24 hours, after 3% pentobarbital sodium 30mg/kg intraperitoneal injection of anesthesia, abdominal aortic blood, rapid broken end gets brain, cerebral tissue take out be placed on ﹣ 20 DEG C cryogenic refrigerator in quick freezing 20min, then freezing cerebral tissue is removed olfactory bulb, cerebellum and low brain stem, is crownly cut to 4 cuttves, be divided into five deciles, the thick 2mm of every sheet.Brain sheet is placed in glass dish, adds the TTC solution of 1%, in the water-bath of 370C, hatch 20min, measure the body weight of infarct location and the body weight of brain of 5 brains after colour developing completely, cerebral infarction index is infarct body weight and the ratio of whole brain body weight.
3 results
The impact of 3.1 medicines on rat function score is compared with sham operated rats, and model group Neuroscore obviously increases, and rat cerebral ischemia modelling success is described; Compare with model group, obviously reduce (p<0.01) by the heavy dose of Neuroscore of reagent.The results are shown in Table 1.
Table 1 medicine on the impact of Level In Rats With Focal Cerebral Ischemia function of nervous system (
± s)
* p<0.01 is compared with model group
The impact of 3.2 medicines on rats with cerebral ischemia cerebral infarction index is compared with sham operated rats, and ischemia model group rat cerebral infarction index obviously raises (p<0.05); Compare with model group, the large, medium and small each dosage group cerebral infarction index of medicine all obviously reduces (p<0.05).The results are shown in Table 2.
Table 2 medicine on the impact of Level In Rats With Focal Cerebral Ischemia cerebral infarction index (
± s)
* * P<0.01 is compared, * p<0.05 with model group
4 conclusions
The middle cerebral artery line brush of rat because of its have without the need to opening cranium, constant infarct can be produced, reproducible, can the different conditions such as the permanent ischemia of simulating human and ischemia-reperfusion] etc. advantage and become the most frequently used focal cerebral ischemia in rats.
Neuroscore reflects the degree of injury of function of nervous system after ischemia, is one of most important index judging drug effect.Cerebral infarction volume evaluates the most direct index of anti-cerebral ischemia effect.
This test adopts line brush to block side middle cerebral artery and causes mesencephalic arteries thromboembolism, make corresponding blood supply district ischemia, anoxia, energy exhaustion and cause cerebral cell ischemia downright bad, make rat occur delayed ischemic neurological deficits, show as Neuroscore to raise, cerebral infarction indexes Index increases.Test medicine obviously can alleviate the delayed ischemic neurological deficits of rats with cerebral ischemia, and reduce cerebral infarction body weight, focal cerebral ischemic injury has significant protective effect.
More than show and describe ultimate principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and description just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (4)
1. Rhizoma Acori Graminei, Semen Myristicae and Flos Caryophylli extract an extracting method for mixture, it is characterized in that, comprise the following steps:
Step 1: refluxed by the mixture of Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli, prepares Rhizoma Acori Graminei, Semen Myristicae, the Mixed chaotic sequences of Flos Caryophylli, filtrate and medicinal residues, and volatile oil and beta-schardinger dextrin-is carried out enclose, obtain the Benexate Hydrochloride of volatile oil;
Step 2, the medical material slag in step 1 is added 10 times of water gaging heating and refluxing extraction 1.5 hours again, squeeze clean medicinal liquid, filtered while hot; Filtrate filtrate and step 1 obtained merges, hold over night under not higher than 15 DEG C of environment, and after collecting the floating yellow solid oils and fats of solution, remaining solution Rotary Evaporators concentrates evaporate to dryness, obtains dry extract for subsequent use, for subsequent use after yellow solid oils and fats dehydrate;
Step 3, medicinal residues remaining in step 2 95% alcohol heating reflux of 5 times amount is extracted 2 times, extraction time is respectively 6h, 4h, heat filtering, merging filtrate, 55 DEG C-75 DEG C decompression distilleds, and the oven dry of 50-60 DEG C, concentrated solution baking oven, obtains dry extract for subsequent use;
The dry extract prepared in step 4, dry extract step 2 obtained and dried oils and fats and step 3 is all ground into fine powder, and the Benexate Hydrochloride of the volatile oil obtained with step 1 mixes, and obtains Rhizoma Acori Graminei, Semen Myristicae and Flos Caryophylli and extracts mixture.
2. Rhizoma Acori Graminei according to claim 1, Semen Myristicae and Flos Caryophylli extract the preparation method of mixture, it is characterized in that, in described step 1, the mixture of Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli is refluxed, prepares Rhizoma Acori Graminei, Semen Myristicae, the Mixed chaotic sequences of Flos Caryophylli, filtrate and medicinal residues and be specially:
Step 1.1, Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli three kinds of processing of crude drugs are become fragment, be that the ratio of 2-4:1-3:1 mixes with mass ratio, add the water of medical material volume about 10 times amount, reflux, extract, in multifunctional extracting pot also collects volatile oil; After anhydrous sodium sulfate dehydration, obtain the Mixed chaotic sequences of Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli three kinds of medical materials, for subsequent use;
When step 1.2, the volatilization oil mass distillated that continues to be back to no longer increase, continue reflux, extract, 2.0 hours, leach filtrate while hot, separately put, medicinal residues are separately put;
Step 1.3, the volatile oil raised is adopted polishing beta-cyclodextrin inclusion compound: the anhydrous alcohol solution volatile oil solution being made into volume fraction 50% with anhydrous alcohol solution volatile oil, get beta-schardinger dextrin-, add after 4 times amount distilled water grind well, slowly add the anhydrous alcohol solution volatile oil solution of volume fraction 50%, hand lapping 3h is to becoming pasty state, sucking filtration, clathrate petroleum ether rinses 3 times, each 10ml, 40 DEG C of vacuum drying 3-8h, the Benexate Hydrochloride of volatile oil must be done, for subsequent use.
3. Rhizoma Acori Graminei according to claim 2, Semen Myristicae and Flos Caryophylli extract the preparation method of mixture, and it is characterized in that, the particle diameter of described Rhizoma Acori Graminei, Semen Myristicae, Flos Caryophylli three kinds of medical materials is 0.5cm.
4. Rhizoma Acori Graminei according to claim 2, Semen Myristicae and Flos Caryophylli extract the preparation method of mixture, and it is characterized in that, the mass volume ratio (g/ml) of the beta-schardinger dextrin-in described step 1.3 and volatile oil is 4:1-8:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510791816.5A CN105232831A (en) | 2015-11-17 | 2015-11-17 | Application of extract mixture of Acorus gramineus Soland., nutmeg and clove to treatment of cerebral ischemia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510791816.5A CN105232831A (en) | 2015-11-17 | 2015-11-17 | Application of extract mixture of Acorus gramineus Soland., nutmeg and clove to treatment of cerebral ischemia |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105232831A true CN105232831A (en) | 2016-01-13 |
Family
ID=55030825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510791816.5A Pending CN105232831A (en) | 2015-11-17 | 2015-11-17 | Application of extract mixture of Acorus gramineus Soland., nutmeg and clove to treatment of cerebral ischemia |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105232831A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110638908A (en) * | 2019-09-27 | 2020-01-03 | 宁夏医科大学 | Traditional Chinese medicine composition for treating cerebral arterial thrombosis and preparation method thereof |
| CN109819980B (en) * | 2019-04-02 | 2021-04-06 | 哈工大机器人（山东）智能装备研究院 | Slow-release mosquito-proof insect-repellent incense bead and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101590152A (en) * | 2008-05-26 | 2009-12-02 | 内蒙古医学院 | The preparation method of Bawei Chenxiang capsule |
| CN102085252B (en) * | 2011-01-21 | 2012-07-11 | 中国人民解放军第二军医大学 | Pharmaceutical composition for treating ulcerative colitis and colon-targeted micro-pill preparation thereof |
| CN103055064A (en) * | 2011-10-23 | 2013-04-24 | 天津大学 | Pharmaceutical composition for treating cerebral apoplexy and preparation method |
| CN104288390A (en) * | 2014-10-29 | 2015-01-21 | 内蒙古天奇中蒙制药股份有限公司 | Preparation method for medicine capable of treating cerebral infarction |
-
2015
- 2015-11-17 CN CN201510791816.5A patent/CN105232831A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101590152A (en) * | 2008-05-26 | 2009-12-02 | 内蒙古医学院 | The preparation method of Bawei Chenxiang capsule |
| CN102085252B (en) * | 2011-01-21 | 2012-07-11 | 中国人民解放军第二军医大学 | Pharmaceutical composition for treating ulcerative colitis and colon-targeted micro-pill preparation thereof |
| CN103055064A (en) * | 2011-10-23 | 2013-04-24 | 天津大学 | Pharmaceutical composition for treating cerebral apoplexy and preparation method |
| CN104288390A (en) * | 2014-10-29 | 2015-01-21 | 内蒙古天奇中蒙制药股份有限公司 | Preparation method for medicine capable of treating cerebral infarction |
Non-Patent Citations (1)
| Title |
|---|
| 河南羚锐制药股份有限公司编著: "<<大别山药物志略>>", 31 July 2010, 河南科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109819980B (en) * | 2019-04-02 | 2021-04-06 | 哈工大机器人（山东）智能装备研究院 | Slow-release mosquito-proof insect-repellent incense bead and preparation method thereof |
| CN110638908A (en) * | 2019-09-27 | 2020-01-03 | 宁夏医科大学 | Traditional Chinese medicine composition for treating cerebral arterial thrombosis and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102836177B (en) | Traditional Chinese medicine preparation for treating osteopathia | |
| CA3219768A1 (en) | Pharmaceutical composition for treating rheumatoid arthritis and preparation method therefor | |
| CN102846975B (en) | Medicine composition as well as application and preparation of medicine composition | |
| CN100447148C (en) | Cape jasmine extract , its preparing process and application | |
| CN103263529B (en) | Traditional Chinese medicine composition for treating bone fracture | |
| CN105232831A (en) | Application of extract mixture of Acorus gramineus Soland., nutmeg and clove to treatment of cerebral ischemia | |
| CN102641278A (en) | Application of ginkgolic acid in treating arthritis | |
| CN108815488B (en) | Compound essential oil preparation for treating dysmenorrhea and preparation method and using method thereof | |
| KR101407611B1 (en) | Dioscoreae Rhizoma comprising the manufacture of a medicament for the treatment of facial nerve paralysis, and the extract beongbeop | |
| CN102038913A (en) | Oral preparation for treating cerebral apoplexy and preparation method and application thereof | |
| CN1067901C (en) | Drug for curing migraine and its preparing method | |
| CN106902317A (en) | It is a kind of to treat Chinese medicine composition of depression and preparation method thereof | |
| CN106620543B (en) | Application of curcuma kwangsiensis extract with antithrombotic effect | |
| CN113244356A (en) | Traditional Chinese medicine composition for treating psoriasis | |
| CN102406792B (en) | Pharmaceutical composition for rapidly promoting healing of bone fracture | |
| CN101700385B (en) | Pharmaceutical composition medicinal liquor or tincture for curing white vein disease and preparation method thereof | |
| CN109646539B (en) | Traditional Chinese medicine composition for improving local circulatory disturbance and preparation method and application thereof | |
| CN102940656B (en) | Medicine composition for treating benign prostatic hyperplasia | |
| CN111000902B (en) | Traditional Chinese medicine tincture for treating acute soft tissue injury and preparation method thereof | |
| CN102727724B (en) | Traditional Chinese medicine composition for dissolving thrombus and preparation method of composition | |
| CN107375700A (en) | A kind of herbal composition preparation with prevention and treatment male infertility infertility | |
| CN104189336A (en) | Medicine composition for treating osteoproliferation | |
| CN101264302A (en) | Application of Chinese medicinal composition in preparing medicaments for treating menoxenia and menorrhalgia | |
| CN103156940B (en) | A kind of extracting technique of Chinese medicine for garden balsam stem pain-relieving | |
| CN106729101B (en) | Traditional Chinese medicine composition for treating post-circulation ischemic stroke and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160113 |