CN105211100A - A kind of plant resource acetum pyrolignosum rectificatum liquid is to the preparation method of root knot nematode control preparation - Google Patents
A kind of plant resource acetum pyrolignosum rectificatum liquid is to the preparation method of root knot nematode control preparation Download PDFInfo
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- CN105211100A CN105211100A CN201510593160.6A CN201510593160A CN105211100A CN 105211100 A CN105211100 A CN 105211100A CN 201510593160 A CN201510593160 A CN 201510593160A CN 105211100 A CN105211100 A CN 105211100A
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Abstract
The invention discloses a kind of plant resource acetum pyrolignosum rectificatum liquid to the preparation method of root knot nematode control preparation, wherein acetum pyrolignosum rectificatum liquid adopts sterile water to be diluted to 1:25-150.Containing the composition of but root-knot nematode egg tool being incubated to activity and eelworm-killing activity in acetum pyrolignosum rectificatum liquid, but thus acetum pyrolignosum rectificatum liquid has long-acting incubating activity and kill line activity, in control root-knot nematode disease field, there is Study and appliance to be worth and DEVELOPMENT PROSPECT, acetum pyrolignosum rectificatum liquid does not pollute soil, beneficial element wherein after plant completes vegetative stage still in action, it has also adsorbed the heavy metal element in soil, plays positive effect to improvement soil.
Description
Technical field
The present invention relates to control of plant disease technical field, specifically a kind of plant resource acetum pyrolignosum rectificatum liquid is to the preparation method of root knot nematode control preparation.
Background technology
Root-knot nematode is one of important pathogen biology harmed the crops.Agriculturally mainly adopt chemical control to control plant nematode diseases. but because most of nematocide has higher toxicity, dangerous to people and animals, what have also has generally killing livestock property, and Long-Time Service has a significant impact environment.Root-knot nematode is an important plant pathogeny line insect, all has distribution all over the world, and root-knot nematode parasitizes various higher plant root, when it takes food with when breeding in the root of plant, induction can produce orbicule or root knot, upset and infected the physiology of plant, reduce output and product quality.
In order to control nematodiasis, the research of some biologic products and organic matter reagent and safe handling method aspect thereof makes some progress, but due to the very complicated and ubiquity of soil fungistasis of soil environment, in limiting the performance of microorganism formulation effect to a great extent.Therefore still need at present to develop some effectively, the product of environmental sound or compound be for preventing and treating plant nematode diseases.Since the sixties in 20th century starts to extract natural nematocidal active material from plant, the work of exploitation plant resource nematocide causes researcher's great interest.
Crude pyroligneous acid is as the accessory substance of wood carbonization, application prospect in the comprehensive utilization of discarded object and environmental protection and Agricultural Sustainable Development has attracted the concern of researchers, pyroligneous liquor is the filbert transparent liquid of acidity containing multiple organic compound obtained by wood destructive distillation, in pyroligneous liquor except main component acetic acid, also less containing content, miscellaneous ketone compounds, phenolic compound, the compound such as aldehydes and alcohols, pyroligneous liquor is used to agricultural in the world, livestock breeding, the domestic research to pyroligneous liquor is less so far, to the research that pyroligneous liquor Promoting plant growth aspect has some preliminary, but but rarely have report with regard to the aspect of pyroligneous liquor to the effect of incubating of root-knot nematode egg.
Summary of the invention
The object of the invention is to disclose a kind of plant resource acetum pyrolignosum rectificatum liquid to the preparation method of root knot nematode control preparation.
For achieving the above object, the invention provides following technical scheme:
Plant resource acetum pyrolignosum rectificatum liquid is to a preparation method for root knot nematode control preparation, and wherein acetum pyrolignosum rectificatum liquid adopts sterile water to be diluted to 1:25-150.
As the further scheme of the present invention: described acetum pyrolignosum rectificatum liquid adopts sterile water to be diluted to 1:75-120.
As the present invention's further scheme: described acetum pyrolignosum rectificatum liquid adopts sterile water to be diluted to 1:100.
Compared with prior art, the invention has the beneficial effects as follows:
Containing the composition of but root-knot nematode egg tool being incubated to activity and eelworm-killing activity in acetum pyrolignosum rectificatum liquid, but thus acetum pyrolignosum rectificatum liquid has long-acting incubating activity and kill line activity, in control root-knot nematode disease field, there is Study and appliance to be worth and DEVELOPMENT PROSPECT, acetum pyrolignosum rectificatum liquid does not pollute soil, beneficial element wherein after plant completes vegetative stage still in action, it has also adsorbed the heavy metal element in soil, plays positive effect to improvement soil.
Embodiment
Be described in more detail below in conjunction with the technical scheme of embodiment to this patent.
Plant resource acetum pyrolignosum rectificatum liquid is to a preparation method for root knot nematode control preparation, and wherein acetum pyrolignosum rectificatum liquid adopts sterile water to be diluted to 1:75-100.
The acetum pyrolignosum rectificatum liquid of variable concentrations is adopted to carry out suppression hatching test to root-knot nematode egg, acetum pyrolignosum rectificatum liquid sterile water is diluted to 1:25,1:50,1:75,1:100,1:150 and 1:200 and contrasts with sterile water, observe at 12h, 24h, 48h, 72h, 96h, 144h respectively, result of the test shows, but incubating of different dilution factor pyroligneous liquor performance is active different, during dilution factor lower than 100 times, the non-incubation rate of root-knot nematode egg is higher, but dilution 150 times more than incubate activity start reduction; The desirable dilution factor of pyroligneous liquor inhibition is between 75 times and 100 times, and the best dilution factor of practical application effect is 100 times.When lower than 100 times of dilution factors, the second instar larvae survival rate of just having hatched after 24h is almost nil, and under same extension rate, inhibit activities increases gradually along with the prolongation of time; Test as follows:
1. materials and methods:
1.1 materials:
1.1.1 for having a try agent
Crude pyroligneous acid is taked from Kuming XianDi Biology Science Co., Ltd, and charing timber is based on the broad-leaved tree timber of toothed oak wood, and the pyroligneous liquor after taking leaves standstill the raw material that 6 months are used as this experiment later in hold-up tank.PH value is about 4.0.
1.1.2 for examination nematode: root-knot nematode is cultivated by Plant nematode research department of Agricultural Biodiversity engineering center, and host is susceptible kind tomato.
1.2 methods:
1.2.1 for the preparation of examination line eggs: the tomato root 100g getting egg capsule more shreds, and is placed in the 1%NaOCL solution that agitator adds 200ml, but discrete for measuring the ovum of incubating activity.Turn on agitator 45s, wash with sterile water immediately in the water-butt of 15L and dilute, suspension is respectively fast the screen cloth of 75 μm and 26 μm through aperture, 26 μm of screen clothes are inverted in another 15L water-butt rinse, by suspension 26 μm of screen filtrations through aperture again, 26 μm, aperture screen cloth can collect free ovum, free ovum is suspended from 20ml sterile water, take out 5ml suspension and be configured to the suspension system that polypide density is 100/ml, be all stored in the refrigerator of 4 DEG C for subsequent use.
1.2.2 the preparation of acetum pyrolignosum rectificatum liquid: settled process prepares method the most simple and effective in acetum pyrolignosum rectificatum liquid method, crude pyroligneous acid poured into the container of 2L, leaves standstill about six months.In the process left standstill, the labile element in pyroligneous liquor is oxidized, polymerization etc. acts on and precipitates.The clarified solution getting top is acetum pyrolignosum rectificatum liquid.Due to long-term placement, need to select acid proof material.If think acquisition in the short time, need the process for separation and purification such as application a series of such as distillation, organic complex extraction.
1.2.3 the dilution of acetum pyrolignosum rectificatum liquid: the system taking 150ml as obtain solution, 6 dilution gradients are set, namely dilution preparation is carried out with 25 times, 50 times, 75 times, 100 times, 150 times, 200 times respectively, the test solution configured is placed in brown, wide-mouth bottle, keep in Dark Place for subsequent use, preferably now with the current, the solution holding time prepared is not easily more than 15d.
1.2.4 determination of activity: adopt infusion process, diameter is selected to be the matter transparent surface ware of 6cm, add the bacterium root-knot nematode egg suspension 1ml of 100/ml with liquid-transfering gun in each surface plate, the obtain solution that 3ml has diluted is added again in surface plate, surface plate is placed in culture dish, be put in 26 DEG C of constant incubators and cultivate, take sterile water as contrast, this experiment comprises contrast 7 process, 5 repetitions are established in each process, totally 35 samples, respectively at 12h, 24h, 48h, 72h, 96h, 144h observes the situation of egg hatching under surface plate being placed in stereomicroscope and counts, the second instar larvae hatched is chosen simultaneously, certain hour is brought back to life in clear water, calculate the survival rate of root-knot nematode second instar larvae under the different dilution factor of acetum pyrolignosum rectificatum liquid respectively.When calculating incubation rate, each process ovum number is 100, and computing formula is as follows:
During incubation rate=100 process, worm amount × 100% non-incubation rate=100-incubation rate of hatching corrects non-incubation rate=1-and contrasts second instar larvae that the non-incubation rate of non-incubation rate process-the contrast second instar larvae hatched in the survival rate=process of non-incubation rate × 100% hatches and to live worm × 100%.
1.2.5 data statistic analysis: adopt SAS8.0 to carry out one-way analysis of variance.
2. results and analysis:
But 2.1 acetum pyrolignosum rectificatum liquid incubate effect to root-knot nematode egg: the process below acetum pyrolignosum rectificatum liquid 100 times of dilution factors only has the egg hatching of only a few to go out second instar larvae, it is dead when part larva is not also broken chorion and goes out, in encircling shape, ovum some change inner can be found out under the microscope, the nematode larval chamber structure division of non-broken shell is cleared up, and produces vacuole simultaneously.With 75 times with 50 times of acetum pyrolignosum rectificatum liquid dilution process time, rarely larva breaks chorion, even if just hatch, polypide is also superfine, and very fast dead, and polypide chamber structure is cleared up in a large number, and body wall is separated with intestines.
Acetum pyrolignosum rectificatum liquid reduces by its extension rate order the inhibitory action of root-knot nematode egg hatching after 144h.Lower lower than root-knot nematode egg hatching rate during 100 times of dilution factors, from dilution 25 doubly to 100 times, but they incubate difference between the effects significantly (P<0.05) to root-knot nematode egg, are in a level, the best dilution factor of practical application effect is 100 times time; During higher than 150 times of dilution factors, the inhibitory action of root-knot nematode egg, in quick reduction, corrects non-incubation rate when dilution 200 times and is low to moderate 46.71%.Essence raising be there is no to the inhibitory action of root-knot nematode egg lower than then increasing application cost when 100 times simultaneously.The process that this experiment is done compared with the control, has the effect significantly suppressing hatching, and has significant difference, and test proves that acetum pyrolignosum rectificatum liquid has the performance suppressing root-knot nematode egg hatching.
Described acetum pyrolignosum rectificatum liquid changes the soil environment of root nematode viability, contact radius PH can change, acetum pyrolignosum rectificatum liquid constantly discharges acetic acid smell, this release is by realizing by moisture, aromatic ammonia spirit has been sucked while plant root oxygen intake, respiratory tract is stimulated to cause reflectivity ground excitation to make nematode constantly suck guaiacol in large quantities, the effect of additional benzoic acid and dead, the mechanism of action of acetum pyrolignosum rectificatum liquid suppresses the breeding of pathogen and the invasion and attack to plant root in soil, and peripheral root nematode is difficult to invade dispenser position, acetaldehyde class in the organic principle of acetum pyrolignosum rectificatum liquid, phenol, ketone carries out sterilization well to root surrounding, sterilization, create good soil environment, root nematode is made to be difficult to existence at this sheet soil.
Containing the composition of but root-knot nematode egg tool being incubated to activity and eelworm-killing activity in acetum pyrolignosum rectificatum liquid, but thus acetum pyrolignosum rectificatum liquid has long-acting incubating activity and kill line activity, in control root-knot nematode disease field, there is Study and appliance to be worth and DEVELOPMENT PROSPECT, acetum pyrolignosum rectificatum liquid does not pollute soil, beneficial element wherein after plant completes vegetative stage still in action, it has also adsorbed the heavy metal element in soil, plays positive effect to improvement soil.
Above the better embodiment of this patent is explained in detail, but this patent is not limited to above-mentioned embodiment, in the ken that one skilled in the relevant art possesses, various change can also be made under the prerequisite not departing from this patent aim.
Claims (3)
1. plant resource acetum pyrolignosum rectificatum liquid is to a preparation method for root knot nematode control preparation, it is characterized in that, wherein acetum pyrolignosum rectificatum liquid adopts sterile water to be diluted to 1:25-150.
2.
rootaccording to plant resource acetum pyrolignosum rectificatum liquid according to claim 1 to the preparation method of root knot nematode control preparation, it is characterized in that, described acetum pyrolignosum rectificatum liquid adopts sterile water to be diluted to 1:75-120.
3. plant resource acetum pyrolignosum rectificatum liquid according to claim 1 is to the preparation method of root knot nematode control preparation, it is characterized in that, described acetum pyrolignosum rectificatum liquid adopts sterile water to be diluted to 1:100.
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| CN106490046A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of prevent and treat agricultural chemicals of root knot nematode disease and preparation method thereof |
| CN108432819A (en) * | 2018-02-13 | 2018-08-24 | 平南县亿翔环保能源有限公司 | A kind of wood vinegar meloidogyne pesticide |
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Application publication date: 20160106 |