WO2010150034A2 - Plant conditioning composition of natural origin and method of application thereof - Google Patents
Plant conditioning composition of natural origin and method of application thereof Download PDFInfo
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- WO2010150034A2 WO2010150034A2 PCT/HU2010/000075 HU2010000075W WO2010150034A2 WO 2010150034 A2 WO2010150034 A2 WO 2010150034A2 HU 2010000075 W HU2010000075 W HU 2010000075W WO 2010150034 A2 WO2010150034 A2 WO 2010150034A2
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- 230000003750 conditioning effect Effects 0.000 title claims abstract description 15
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- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
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- AFSDNFLWKVMVRB-UHFFFAOYSA-N Ellagic acid Chemical compound OC1=C(O)C(OC2=O)=C3C4=C2C=C(O)C(O)=C4OC(=O)C3=C1 AFSDNFLWKVMVRB-UHFFFAOYSA-N 0.000 description 1
- ATJXMQHAMYVHRX-CPCISQLKSA-N Ellagic acid Natural products OC1=C(O)[C@H]2OC(=O)c3cc(O)c(O)c4OC(=O)C(=C1)[C@H]2c34 ATJXMQHAMYVHRX-CPCISQLKSA-N 0.000 description 1
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- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
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- NGFMICBWJRZIBI-JZRPKSSGSA-N Salicin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O1)c1c(CO)cccc1 NGFMICBWJRZIBI-JZRPKSSGSA-N 0.000 description 1
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- 241000207763 Solanum Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
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- 230000009471 action Effects 0.000 description 1
- NGFMICBWJRZIBI-UHFFFAOYSA-N alpha-salicin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UHFFFAOYSA-N 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
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- 238000010352 biotechnological method Methods 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 150000008280 chlorinated hydrocarbons Chemical class 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229960002852 ellagic acid Drugs 0.000 description 1
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- 230000002538 fungal effect Effects 0.000 description 1
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- 229940074391 gallic acid Drugs 0.000 description 1
- 235000002532 grape seed extract Nutrition 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 229960001340 histamine Drugs 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
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- 230000000670 limiting effect Effects 0.000 description 1
- FAARLWTXUUQFSN-UHFFFAOYSA-N methylellagic acid Natural products O1C(=O)C2=CC(O)=C(O)C3=C2C2=C1C(OC)=C(O)C=C2C(=O)O3 FAARLWTXUUQFSN-UHFFFAOYSA-N 0.000 description 1
- 150000003014 phosphoric acid esters Chemical class 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- NGFMICBWJRZIBI-UJPOAAIJSA-N salicin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UJPOAAIJSA-N 0.000 description 1
- 229940120668 salicin Drugs 0.000 description 1
- 150000003872 salicylic acid derivatives Chemical class 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
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- 231100000331 toxic Toxicity 0.000 description 1
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- 239000000341 volatile oil Substances 0.000 description 1
- 239000009637 wintergreen oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Definitions
- Plant conditioning composition of natural origin and method of application thereof
- the object of the invention is an environment-friendly plant condition composition of natural origin and method for the application thereof for the protection of cultivated plants, mainly green pepper, tomato, cucumber, grapevine and pomes against pests.
- Patent No. HU 223 266 induction of viral resistance was attempted by biotechnological methods
- Patent No. HU 220 582 new strain of Pseudomonas chlororaphis bacterium was used as active ingredient in the protection against plant diseases.
- Patent No.s HU 214 217 and HU 207 855 relate to the plant protection agent preparations containing chemically synthesized salicylic acid derivative. However, these solutions are basically chemical plant protection agent preparations containing conventional, chemically prepared active ingredient.
- Plants specifically sensitive for these kinds of diseases are for example green pepper, tomato, cucumber, grapevine and pomes, many of them are cultivated even in greenhouses.
- the goal of the invention is to find solution for the problems mentioned above, without the disadvantages outlined above.
- the invention is based on the discovery that the effect needed should not necessarily be reached by killing the fungi or viruses, but rather by increasing the resistance of the plant with substances harmless for the human and animal health, and for the environment, i.e., we have to turn to some kind of prevention, but in addition to the usual nutrient supply this needs agents enhancing the condition of the plant.
- the preparation according to the invention can also raise the physiological characteristics, resistance of the plant to higher level.
- Salix fragilis Crack Willow
- Salix triandra Almond-leaved Willow
- Applicable plant parts bark, shoot, water extract of leaf drug
- Content salicin, glycosides, catechin tannin, resin
- Poplar Populus nigra (Lombardy poplar)
- Populus alba White poplar
- Populus robusta robusta poplar
- Applicable plant parts water extract of fresh leaf, flower-bud; Content: Populi gemma, volatile oils, resin, acids; c/ Nuts (Junglandaceae): Junglands regia (walnut)
- Applicable plant parts leaf and green-nuts and cropshell (nut pericarp);
- tannin tannin
- gallic acid ellagic acid, inositol, junglon bitter, Bitter substance, junkglandine-alkaloid
- d/ Nettles Urtica dioica (stinging nettle, big nettle)
- Applicable plant parts leaf drug (Urtica folium); Content: tan substances, resin substance (nettle toxin), glycosides, formic acid, histamine, glycokinin.
- the mixture of water extracts of plants a/-d/ is the most effective, if it contains at least 50% of the extracts a/ and b/, and about the same amount, but at least 10-10% of extracts c/ and d/ is present in the ready-made, undiluted spray, calculated to its total amounts.
- each extract component (a/-d/) is characterized by the fact that the suitable extract contains the extract of at least 20 w% plant parts prepared with at most 80 w% water, wherein the optional dried substance (i.e. bark substance, phloem) must be taken into consideration with a multiplication factor of three (i.e. 1 kg dried substance is equivalent to 3 kg fresh plant parts).
- the optional dried substance i.e. bark substance, phloem
- the object of the invention is a plant conditioning preparation of natural origin, and application therefore for the protection of cultivated crops, mainly pepper, tomato, cucumber, grapevine and pomes against pests.
- the preparation is characterized that it contains pooled water extracts of four plants a/ Willows (Salicaceae), b/ Poplar (Salicaceae), c/ Nuts (Junglandaceae) and d/ Nettles (Urticaceae), wherein the amount of the pooled extracts of a/ and b/ amounts to at least 50 w%, the amount of c/ and d/ each amounts to at least 10-40 w%, but altogether at most 50 % of the spray, wherein the extracts are prepared from at least 20 w% plant parts prepared with at most 80 w% water.
- the preparation of the invention optionally includes additives and/or excipients known per se. Realization of the preparation of the invention and the application are illustrated through the following examples, without limiting the scope of
- Example 1 To prepare plant conditioning composition of natural origin the following are prepared:
- water extract measured in an extracting container with dense woven gauze sidewall, in a procedure similar to the procedure in a/, water extract is prepared with 70 parts by mass water heated to 70 0 C, and is allowed to flow through a filter into a separate storage tank.
- the plots were prepared with seeds of 98% germinative ability planted with 5 pcs/m seed density (April 24), the shooting date can be measured from May 03. Treatment starts from May 15, in every 15 days with 400 I/hectare dose.
- 0,05% pepper mosaic (Tobacco mosaic virus) 0,08% pepper stolbur (Stolbur mycoplasma) diseases, later on the untreated harvest bacterial (Pseudomonas syringae var.
- capsici diseases were observed
- the preparation containing component a/ reduced the viral infection compared to the control, and in the plots treated with the spray of a/ + b/ + c/ + d/ composition according to the invention the viral infection significantly decreased, and by the effect of the treatment at the occurrence of the first flowerbuds primary bacterial (Pseudomonas) infection didn't occur.
- the stems visibly started growing, r s leaves devastation didn't occur, the occurring harvest initiatives were healthy, phytotoxic effect could not be observed.
- a preparation containing a mixture of 70 kg a/ + 10 kg b/ + 10 kg c/ + 10 kg d/ is prepared.
- a 1,0% water solution of this mixture is used for spraying the experimental plots of potato (Solanum tuberosum) Gulbaba and Kisvardai r ⁇ zsa variety, and the untreated control plots, and the
- the plots were prepared with potatoes of 100% germinative ability planted with 5 pcs/m density, 15 cm deep planting (Aprii 24), after planting date (April 22) the shooting date could be measured from May 03. Treatment starts from May 15,
- the preparation containing components a/ + b/, at strong infection pressure reduced the degree of altemaria (Altemaria porri f.sp solani) infection, compared to the control, but the preparation of the invention containing a/ + b/ + c/ + d/ components showed significantly higher protective effect even in case of Phytophtora infestans, Ervinia cartovovora var.
- the applied procedure is similar to the one used in Examples 1 and 3.
- a preparation containing a mixture of 70 kg a/ + 10 kg b/ + 10 kg c/ + 10 kg d/ is prepared, and a 1 ,5% water solution of this mixture is used for spraying the experimental plots of cucumber (Cucumis sativus).
- the untreated control plots, and the plots treated only with spraying with only components a/ + b/ (willow + birch extract) are also observed.
- the preparation containing components a/ + b/, at strong infection pressure reduced the degree of alternaria (Alternaria porri f.sp solani) infection, compared to the control, but the preparation of the invention containing a/ + b/ + c/ + d/ components showed significantly higher protective effect.
- the efficacy of the a/, b/, c/, d/ extracts was investigated separately, and it has been found that any extract is not as efficient as the preparation of the invention containing a/ + b/ + c/ + d/ components, which can be used against this damaging cucumber disease with very good results.
- the applied procedure is similar to the one used in Example 1.
- a preparation containing a mixture of 60 kg a/ + 10 kg b/ + 15 kg c/ + 15 kg d/ is prepared.
- An 0,4% water solution of this mixture is used for spraying the sylvoz cultivated plantation of vine (Vitis vinifera) of Furmint species.
- Efuzin 500 FW, 1 liter/ha spraying was used as comparative treatment.
- the degree of infection appeared to be medium at the first treatment, irregular specks occurred on the leaves, which died, necrotized, and fell off the leaf-blade after the first treatment, In case of the untreated control the degree of infection is 50-73%, in case of Efuzin 500 WF it is 15-23%, in case of the preparation of the invention it is 8-12%, phytotoxic effect could not be observed.
- the applied procedure is similar to the one used in Example 1.
- a preparation containing a mixture of 60 kg a/ + 10 kg b/ + 15 kg c/ + • 15 kg d/ is prepared.
- An 0,4% water solution of this mixture is used for spraying the plantation of apple (Malus domesticus) Gala species, Efuzin 500 FW, 1 litre/ha spraying was used as comparative treatment. Dates of treatments were May 15, May 25 and June 20, the amount of the spray was 600 litre/hectare.
- the degree of scabbing (Venturia inequalis), and the degree of apple tree powdery mildew (Podosphaera leucotrichal) were measured in the percentage of being infected of the leaves: in case of untreated control at the first treatment 55%, at the second treatment 72% occurred, in case of Efuzin 500 WF after the first treatment 22%, after the second treatment 14%, while after the treatments with the preparation of the invention these were 20% or 9%.
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The object of the invention is a plant conditioning preparation of natural origin, for the protection of cultivated crops, mainly pepper, tomato, cucumber, grape-vine and pomes against pests, characterized that the preparation - optionally with one or more additives and/or excipients, known per se - contains pooled water extracts of the following four plants a/ Willows (Salicaceae), b/ Birches (Betulaceae), c/ Nuts (Junglandaceae) and d/ Nettles (Urticaceae), wherein the amount of the combined extracts of a/ and b/ amounts to at least 50 w%, the amount of c/ and d/ each amounts to at least 10-40 w%, but altogether at most 50 % of the spray, wherein the extracts are prepared from at least 20 w% plant parts prepared with at most 80 w% water. Additionally the object of the invention is the use of the plant conditioning preparation, characterized that the preparation is used at the site of application as a 0,1-5% diluted water spray.
Description
705644/DE
Plant conditioning composition of natural origin and method of application thereof
The object of the invention is an environment-friendly plant condition composition of natural origin and method for the application thereof for the protection of cultivated plants, mainly green pepper, tomato, cucumber, grapevine and pomes against pests.
It is known, that the successfulness of agriculture may be greatly reduced by the pests attacking the cultivated plants, the viral, bacterial and fungal diseases. Even in the last century preparations containing synthetic chemical compounds, i.e. chlorinated hydrocarbons (DDT, HCH), organic phosphoric-acid esters, compounds containing sulphur and phosphor, and even toxic heavy metals (Bi, Hg1 Tl), which were proven to be effective plant protection chemicals, were increasingly used in order to increase the yields to be reached, which at the beginning dramatically improved the yields indeed. Therefore a separate industrial branch was formed for the preparation of the more and more effective and developed (according to different aspects) plant protection agents and preparations, But over the years, particularly in recent times it has become more and more evident, that the agent residues, decomposition products found in the crops intended for feeding or for human consumption may cause health damage with serious consequences, therefore more and more intensive care must be taken for the control of the agent residues, for the determination of the limit values still permissible, and must strive for the development of analytical and biological processes suitable for this purpose. The situation was worsened by the fact, that because of the appearance of the resistance of the pests, application of higher and higher doses was needed, and competition has developed among the companies manufacturing plant protection chemicals for the development and marketing of newer and newer, even more effective chemical agents.
Part of these chemicals accumulates in the plants, and its consumption by animals or humans has serious health damaging risks, since it can initiate the processes of serious illnesses.
As a consequence of the effects mentioned above marketing of numerous plant protection agents containing chemical compounds was banned in many countries.
There were attempts for other solutions, for example in Patent No. HU 223 266 induction of viral resistance was attempted by biotechnological methods, in Patent No. HU 220 582 new strain of Pseudomonas chlororaphis bacterium was used as active ingredient in the protection against plant diseases.
In the situation causing increasing nutrition problems the demand for bio- production and protection increased, where registration of the agricultural land, propagation materials, materials for yield increase and protection, and control of their application is strictly required. The market value of the crops obtained this way can be higher, which can compensate the disadvantages caused by the lower yields caused by the omission of the synthetic products.
It is also known, that some plant species can produce natural substances, which can provide protection against different pests. One known plant of this type is the white willow (Salix alba) belonging to the willows (Salicaceae), which was observed even by the American settlers dealing with basketry, since it turned out about the steeping liquor enhancing the flexibility of the wicker that it is effective against some plant pests (see wintergreen oil, betula oil), The salicylic acid methyl ester could be identified as main active ingredient see: [1] Organic Molecules in
Action, Goodman, Morehouse, Gordon and Breach, 1973; [2] British Pharmaceutical Codex 1911 : METHYLlS SALICYLAS; [3] FAO Nutrition Meetings Report Series No. 44A WHO/Food Add./68.33, Toxicological Evaluation of Same Flavouring Substances and Non-Nutritive Sweetening Agents. It must be noted that the Patent No.s HU 214 217 and HU 207 855 relate to the plant protection agent preparations containing chemically synthesized salicylic acid derivative. However, these solutions are basically chemical plant protection agent preparations containing conventional, chemically prepared active ingredient.
Similarly to the knowledge relating to the willows, it is also well known that the concoction of the leaves of some nettles (Urticaceae) can be used for spraying horticultural plants. The main active ingredients of the concoction: formic acid, tan substances, resinaceous, nettle toxin, etc. For example Patent No.s HU 226 041 and HU 225 901 are relating to the preparations containing among others nettle,
which preparations are recommended on limited application field, in the cultivation of some plants, for example in the application for the preparation of the soil.
The fact is also known that in relation to the first-fruits the risks of the fungal, viral diseases of plants are increasing, since the humid environment with raised temperature in the greenhouse is markedly favourable for their propagation.
Plants specifically sensitive for these kinds of diseases are for example green pepper, tomato, cucumber, grapevine and pomes, many of them are cultivated even in greenhouses.
The goal of the invention is to find solution for the problems mentioned above, without the disadvantages outlined above.
The invention is based on the discovery that the effect needed should not necessarily be reached by killing the fungi or viruses, but rather by increasing the resistance of the plant with substances harmless for the human and animal health, and for the environment, i.e., we have to turn to some kind of prevention, but in addition to the usual nutrient supply this needs agents enhancing the condition of the plant.
But the disadvantage in relation to the known natural substances is that generally these are not sufficiently effective.
In the course of our experiments conducted for increasing the effect of the plant active ingredients of natural origin, and for studying the possibilities of application it was found surprisingly that the effect of the natural protective substances of some plants can be increased by leaps and bounds by supplementing them with the natural protective substances of other specified plant species, when from the aspect of efficacy synergism is generated, i.e. the effect is greater when these are used together, than the effect when these are used sequentially.
In the course of our experiments it was found that if, in addition to the willows (Salicaceae) with the known effect, the mixture of the water extracts of poplar. (Salicaceae), nuts (Junglandaceae) and nettles (Urticaceae) is used, for example for plant conditioning spraying - in this sense with protective effect - the effect can be outstandingly successful, and at the same time the treatment meets the requirements of environment friendly and chemical free protection of the so called bio-cultivation.
In the course of testing the treated plant samples it was found that in the enhanced resistance against plant pesis it can also play an important role that it can be more difficult for the infection to invade the cuticle, as a consequence of the thickening of the epidermis layer, therefore the preparation according to the invention can also raise the physiological characteristics, resistance of the plant to higher level.
For the short characterization of the plant active ingredients the members of the plant families are introduced in the following summary, the applicable plant parts, and the list of the essential and important components of the extracts: a/ Willows (Salicaceae): Salix alba (white willow)
Salix fragilis (Crack Willow) Salix triandra (Almond-leaved Willow) Applicable plant parts: bark, shoot, water extract of leaf drug; Content: salicin, glycosides, catechin tannin, resin; b/ Poplar (Salicaceae): Populus nigra (Lombardy poplar)
Populus alba (White poplar) Populus robusta (robusta poplar)
Applicable plant parts: water extract of fresh leaf, flower-bud; Content: Populi gemma, volatile oils, resin, acids; c/ Nuts (Junglandaceae): Junglands regia (walnut)
Junglands germanica (alpine nuts)
Applicable plant parts: leaf and green-nuts and cropshell (nut pericarp);
Content: tannin; gallic acid, ellagic acid, inositol, junglon bitter, Bitter substance, junkglandine-alkaloid; d/ Nettles (Urticaceae): Urtica dioica (stinging nettle, big nettle)
Applicable plant parts: leaf drug (Urtica folium); Content: tan substances, resin substance (nettle toxin), glycosides, formic acid, histamine, glycokinin.
In the course of our experiments it was found, that the mixture of water extracts of plants a/-d/ is the most effective, if it contains at least 50% of the extracts a/ and b/, and about the same amount, but at least 10-10% of extracts c/
and d/ is present in the ready-made, undiluted spray, calculated to its total amounts.
In the solution according to the invention the concentration of each extract component (a/-d/) is characterized by the fact that the suitable extract contains the extract of at least 20 w% plant parts prepared with at most 80 w% water, wherein the optional dried substance (i.e. bark substance, phloem) must be taken into consideration with a multiplication factor of three (i.e. 1 kg dried substance is equivalent to 3 kg fresh plant parts).
On the basis of what is mentioned above the object of the invention is a plant conditioning preparation of natural origin, and application therefore for the protection of cultivated crops, mainly pepper, tomato, cucumber, grapevine and pomes against pests. The preparation is characterized that it contains pooled water extracts of four plants a/ Willows (Salicaceae), b/ Poplar (Salicaceae), c/ Nuts (Junglandaceae) and d/ Nettles (Urticaceae), wherein the amount of the pooled extracts of a/ and b/ amounts to at least 50 w%, the amount of c/ and d/ each amounts to at least 10-40 w%, but altogether at most 50 % of the spray, wherein the extracts are prepared from at least 20 w% plant parts prepared with at most 80 w% water. The preparation of the invention optionally includes additives and/or excipients known per se. Realization of the preparation of the invention and the application are illustrated through the following examples, without limiting the scope of the invention to the examples.
Example 1 To prepare plant conditioning composition of natural origin the following are prepared:
Water extract from the suitable plant parts of a/ willows (Salicaceae), b/ poplar (Salicaceae), c/ nuts (Junglandaceae) and d/ nettles (Urticaceae), carried out practically in a duplicator equipped with steam jacket and stirrer, in the following way: a/ Willows (Salicaceae): from the chopped bark and leafy shoots of Salix alba (white willow) 25 parts by mass is measured in an extracting container with dense woven gauze sidewall, and hanged into a tank equipped with a stirrer containing
75 parts by mass water heated to 50 0C in such a way, that the extracting container containing the chopped material can be mowed up and down with a pulley suspension. In every 5-10 minutes the container is pulled out of and put back into the dissolving water kept moving by the stirrer, the elutriation is
,5 continued for 2 hours, and the container containing the plant parts is finally pulled out of the tank, and the water phase is allowed to flow through a filter into a storage tank. b/ Poplar (Salicaceae): from the dried leaves and flower-buds of the mixture of .white poplar (Populus alba) and Lombardy poplar {Populus nigra) 8 parts by mass
10 is measured in an extracting container with dense woven gauze sidewall, in a procedure similar to the procedure in a/, water extract is prepared with 76 parts by mass water heated to 65 0C, and is allowed to flow through a filter into a separate storage tank, c/ Nuts (Junglandaceae): from the leaves and green-nuts of walnut (Junglands
15 regia) 20 parts by mass is measured in an extracting container with dense woven gauze sidewall, in a procedure similar to the procedure in a/, water extract is prepared with 80 parts by mass water heated to 60 0C, and is allowed to flow through a filter into a separate storage tank. 61 Nettles (Urticaceae): from the chopped leaves and stems 25 parts by mass is
20 measured in an extracting container with dense woven gauze sidewall, in a procedure similar to the procedure in a/, water extract is prepared with 70 parts by mass water heated to 70 0C, and is allowed to flow through a filter into a separate storage tank.
To prepare plant conditioning composition of natural origin for tomato
25 (Solanum jubileum) Mobil, Kecskemeti jubileum species the following spray is prepared:
60 kg willow extract prepared according to a/, 10 kg birch extract prepared according to b/, 15 kg nuts extract prepared according to c/ and 15 kg nettle extract prepared according to d/ are mixed, and spraying is carried out with the
30 0,4% diluted solution of the preparation mentioned above.
In the experimental plot tomato seeds with 96% gerrninative ability are planted with 5 pcs/m seed density (March 22), and after their shooting date (April 03), following replanting (May 03) 300 I/hectare spraying is used in every 15 days.
In the course of the experiments, in addition to the control plots spraying was carried out with a spray containing only component a/, willow extract, and with a spray containing the composition of the invention, with a/ + b/ + c/ + d/ components. In the untreated plot alternaria (Alternaria porri f.sp solani) infection was observed, showing strong infection pressure, in the plots treated with a spray containing only component a/ the alternaria infection decreased, and in the plots treated with the spray of a/ + b/ + c/ + d/ composition of the invention the alternaria infection significantly decreased, and the treatment showed protective effect against Phytophtora infestans, Septoria lycopersici and Xantomonas campestri pv.
Vesicatoria diseases too. Following the first treatment the specks occurring on the sick stems died, necrotized, and fell off the leaf-blade. Following the second treatment the stems visibly started growing, leaves devastation didn't occur. The investigated stand was qualified healthy, compared to the control, phytotoxic effect could not be observed.
Example 2
In a procedure similar to example 1, to prepare plant conditioning composition of natural origin from the extracts of a/ type willows, b/ type poplar, c/ type walnuts and d/ type nettle extracts, a preparation containing a mixture of 70 kg a/ + 5 kg b/ + 12 kg c/ + 13 kg d/ is prepared. An 0,8% water solution of this mixture is used for spraying the experimental plots of pritamin pepper (Capsicum annum) Szentesi Paz species, and the untreated control plots, and the plots treated only with spraying with only component a/ (willow extract) are also observed.
The plots were prepared with seeds of 98% germinative ability planted with 5 pcs/m seed density (April 24), the shooting date can be measured from May 03. Treatment starts from May 15, in every 15 days with 400 I/hectare dose. By the effect of changing temperature and time after flowering on some stems 0,05% pepper mosaic (Tobacco mosaic virus), 0,08% pepper stolbur (Stolbur mycoplasma) diseases, later on the untreated harvest bacterial (Pseudomonas syringae var. capsici) diseases were observed The preparation containing component a/ reduced the viral infection compared to the control, and in the plots
treated with the spray of a/ + b/ + c/ + d/ composition according to the invention the viral infection significantly decreased, and by the effect of the treatment at the occurrence of the first flowerbuds primary bacterial (Pseudomonas) infection didn't occur. By the effect of the following treatments the stems visibly started growing, r s leaves devastation didn't occur, the occurring harvest initiatives were healthy, phytotoxic effect could not be observed.
Example 3
In a procedure similar to example 1 , to prepare plant conditioning io composition of natural origin from the extracts of a/ type willows, b/ type poplar, c/ type walnuts and d/ type nettle extracts, a preparation containing a mixture of 70 kg a/ + 10 kg b/ + 10 kg c/ + 10 kg d/ is prepared. A 1,0% water solution of this mixture is used for spraying the experimental plots of potato (Solanum tuberosum) Gulbaba and Kisvardai rόzsa variety, and the untreated control plots, and the
15 plots treated only with spraying with only component a/ + b/ (willow + birch extract) are also observed.
On the experimental plot, potato has been cultivated and bred continuously for years, by taking into consideration the shift of crops. The nutrient supply of the plot is good; the nutrients are supplemented by drip irrigation. By the effect of
20 changing temperature and time after flowering on some stems 0,85% potato-blight
(Phytophtora infestans) diseases were observed.
The plots were prepared with potatoes of 100% germinative ability planted with 5 pcs/m density, 15 cm deep planting (Aprii 24), after planting date (April 22) the shooting date could be measured from May 03. Treatment starts from May 15,
25 in every 15 days with 400 I/hectare dose. The preparation containing components a/ + b/, at strong infection pressure reduced the degree of altemaria (Altemaria porri f.sp solani) infection, compared to the control, but the preparation of the invention containing a/ + b/ + c/ + d/ components showed significantly higher protective effect even in case of Phytophtora infestans, Ervinia cartovovora var.
30 atroseptica, Corynebacterium sepedonicum diseases. Following the first treatment the specks occurring on the sick stems died, necrotized, and fell off the leaf-blade, following the second treatment the stems visibly started growing, leaves devastation did not occur. From the aspects of plant health the investigated
stand was significantly reduced or made immeasurable, compared to the control. Phytotoxic effect did not occur; the ELISA test used for the detection of the virus detected the potato Y virus only in one tuber from the 20 investigated tubers, therefore the seed tuber produced with this technology qualified to be virus free, and is suitable for propagation.
Example 4
The applied procedure is similar to the one used in Examples 1 and 3. To prepare plant conditioning composition of natural origin from the extracts of a/ type willows, b/ type poplar, c/ type walnuts and d/ type nettle, a preparation containing a mixture of 70 kg a/ + 10 kg b/ + 10 kg c/ + 10 kg d/ is prepared, and a 1 ,5% water solution of this mixture is used for spraying the experimental plots of cucumber (Cucumis sativus). The untreated control plots, and the plots treated only with spraying with only components a/ + b/ (willow + birch extract) are also observed. By proceeding the usual way with spraying in every 15 days with 400
I/hectare dose, the preparation containing components a/ + b/, at strong infection pressure reduced the degree of alternaria (Alternaria porri f.sp solani) infection, compared to the control, but the preparation of the invention containing a/ + b/ + c/ + d/ components showed significantly higher protective effect. In case of the so called cucumber withering (Didimella aplanata) the efficacy of the a/, b/, c/, d/ extracts was investigated separately, and it has been found that any extract is not as efficient as the preparation of the invention containing a/ + b/ + c/ + d/ components, which can be used against this damaging cucumber disease with very good results.
Example 5
The applied procedure is similar to the one used in Example 1. To prepare plant conditioning composition of natural origin from the extracts of a/ type willows, b/ type poplar, c/ type walnuts and d/ type nettle, a preparation containing a mixture of 60 kg a/ + 10 kg b/ + 15 kg c/ + 15 kg d/ is prepared. An 0,4% water solution of this mixture is used for spraying the sylvoz cultivated plantation of vine (Vitis vinifera) of Furmint species. Efuzin 500 FW, 1 liter/ha spraying was used as comparative treatment. At spraying carried out at the beginning of flowering (May
25), at the end of flowering and at the beginning of grape formation (June 15), and before the intensive shoot growing, sprouting, the condition of the vine plantation was evaluated. It could be said, that at the experimental area the amount of precipitation did not reach 20 mm, but the 42 mm precipitation fallen in May resulted, despite of the aridity, in the development of medium level grape powdery mildew (Uncinula necator) and peronospora (Plasmopara viticola) infection. The Metos type forecast instrument installed at the area registered meteorological data and the infection periods. In the period of intensive shoot growth, the instrument detected 5 infections, 2 of them were strong, 2 were medium and 1 was weak. The degree of infection appeared to be medium at the first treatment, irregular specks occurred on the leaves, which died, necrotized, and fell off the leaf-blade after the first treatment, In case of the untreated control the degree of infection is 50-73%, in case of Efuzin 500 WF it is 15-23%, in case of the preparation of the invention it is 8-12%, phytotoxic effect could not be observed.
Example 6
The applied procedure is similar to the one used in Example 1. To prepare plant conditioning composition of natural origin from the a/ type willows, b/ type poplar, ci type walnuts and d/ type nettle extracts, a preparation containing a mixture of 60 kg a/ + 10 kg b/ + 15 kg c/ +• 15 kg d/ is prepared. An 0,4% water solution of this mixture is used for spraying the plantation of apple (Malus domesticus) Gala species, Efuzin 500 FW, 1 litre/ha spraying was used as comparative treatment. Dates of treatments were May 15, May 25 and June 20, the amount of the spray was 600 litre/hectare. The Metos type instrument forecasting scabbing, installed at the area, registered meteorological data and the infection periods. Ir the period of intensive shoot growth the instrument detected 6 infections, 2 of them were strong, 2 were medium and 1 was weak at the time of the first treatment First part of the period investigated was favourablefor Venturia scabbing, because of the amount of the precipitation fallen. This was combined with cool weather, high humidity, followed by a period with very dry air. But the first infections happened, because irregular dark specks occurred on the leaves, At
the end of the investigation the weather conditions were not favourable for the essential conditions of the fungus. The degree of scabbing (Venturia inequalis), and the degree of apple tree powdery mildew (Podosphaera leucotrichal) were measured in the percentage of being infected of the leaves: in case of untreated control at the first treatment 55%, at the second treatment 72% occurred, in case of Efuzin 500 WF after the first treatment 22%, after the second treatment 14%, while after the treatments with the preparation of the invention these were 20% or 9%.
Claims
1. A plant conditioning preparation of natural origin, for the protection of cultivated crops, mainly pepper, tomato, cucumber, grape-vine and pomes against pests, characterized that the preparation - optionally with one or more additives and/or excipients, known per se - contains pooled water extracts of the following four plants a/ Willows (Salicaceae), b/ Poplar (Salicaceae), c/ Nuts (Junglandaceae) and d/ Nettles (Urticaceae), wherein the amount of the pooled extracts of a/ and b/ is at least 50 w%, the amount of c/ and d/ each is at least 10-40 w%, but altogether at most 50 % of the spray, wherein the extracts are prepared from at least 20 w% plant parts prepared with at most 80 w% water.
2. Preparation of Claim 1, characterized that the extract of plants providing one or more components originates from the extraction of fresh plant.
3. Preparation of Claim 1 , characterized that the extract of plants providing one or more components originates from the extraction of dried plants, wherein the amount (mass) of the extracted dry plant amounts to at least one third of the amount (mass) of the respective fresh plant.
4. Preparation of Claim 1 , characterized that the combined extract of components a/ and b/ amounts to at least 60 w% of the combined a/ + b/ + c/ + d/ preparation.
5. Preparation of Claim 1 , characterized that the extract of component c/ (nuts) amounts to at least 30 w% of the combined a/ + b/ + c/ + d/ preparation.
6. Preparation of Claim 1 , characterized that the extract of component ύf (nettles) amounts to at least 30 w% of the combined a/ + b/ + c/ + d/ preparation.
7. Use of a plant conditioning preparation according to any of Claims 1-
5, characterized that the preparation is used at the site of application as a 0, 1-5% diluted water spray.
8. Use of Claim 7, characterized that the preparation is used at the site of application as a 0,2-2% diluted water spray.
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HU0900407A HUP0900407A2 (en) | 2009-06-26 | 2009-06-26 | Plant conditioning composition of natural origin and process for using it |
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CN103004444A (en) * | 2012-12-14 | 2013-04-03 | 冯传荣 | Non-revival-period planting technique for high-yield cultivation of hot peppers |
DE102015111314A1 (en) | 2015-07-13 | 2017-01-19 | Volksbank Straubing eG | Plant strengthening agents, process for their preparation and uses thereof |
WO2017009350A1 (en) | 2015-07-13 | 2017-01-19 | Oget Innovations Gmbh | Plant conditioning composition, method of preparation and uses thereof |
CZ306540B6 (en) * | 2015-09-02 | 2017-03-01 | Botanický Ústav Av Čr, V. V. I. | An extract of Populus nigra and its use for increasing the emergence rate and support of plant growth |
EP3298897A1 (en) * | 2016-09-21 | 2018-03-28 | Gyogynövenykutato Kft | Plant-based plant conditioner and protection product, method of producing such product, and use of such product |
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CN103004444A (en) * | 2012-12-14 | 2013-04-03 | 冯传荣 | Non-revival-period planting technique for high-yield cultivation of hot peppers |
CN103004444B (en) * | 2012-12-14 | 2014-08-27 | 冯传荣 | Non-revival-period planting technique for high-yield cultivation of hot peppers |
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DE102015111314B4 (en) | 2015-07-13 | 2019-05-02 | Oget Innovations Gmbh | Plant strengthening agents, process for their preparation and uses thereof |
US10609929B2 (en) | 2015-07-13 | 2020-04-07 | Oget Innovations Gmbh | Plant conditioning composition comprising water-based and natural oil-based plant extracts |
CZ306540B6 (en) * | 2015-09-02 | 2017-03-01 | Botanický Ústav Av Čr, V. V. I. | An extract of Populus nigra and its use for increasing the emergence rate and support of plant growth |
EP3298897A1 (en) * | 2016-09-21 | 2018-03-28 | Gyogynövenykutato Kft | Plant-based plant conditioner and protection product, method of producing such product, and use of such product |
Also Published As
Publication number | Publication date |
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WO2010150034A3 (en) | 2011-10-06 |
HU0900407D0 (en) | 2009-08-28 |
HUP0900407A2 (en) | 2011-01-28 |
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