CN112369432A - Disinfectant containing wood vinegar and preparation method and application thereof - Google Patents
Disinfectant containing wood vinegar and preparation method and application thereof Download PDFInfo
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- CN112369432A CN112369432A CN202011287565.4A CN202011287565A CN112369432A CN 112369432 A CN112369432 A CN 112369432A CN 202011287565 A CN202011287565 A CN 202011287565A CN 112369432 A CN112369432 A CN 112369432A
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- wood vinegar
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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Abstract
The invention discloses a disinfectant containing wood vinegar, which can be used for disinfecting the environment of livestock and poultry farms and the surfaces of articles. The wood vinegar prepared by adopting a specific process has the best quality of the effective disinfection components, and further achieves the best disinfection effect by the synergistic effect of the wood vinegar, the antioxidant, the metal ion complexing agent and the surfactant, and avoids the adverse effect on the environment of a livestock and poultry farm caused by over-disinfection of the environment.
Description
Technical Field
The invention belongs to the technical field of disinfection, and particularly relates to a disinfectant containing wood vinegar, and a preparation method and application thereof.
Background
The pyroligneous liquor is a reddish brown liquid obtained by condensing and separating a steam gas mixture derived from biomass (wood, fruit pits, wood chips and the like) such as agricultural and forestry waste and the like after dry distillation in dry distillation equipment, and is a natural mixture with very complex components. The main components of the organic phenolic resin are organic acid, phenols, alcohols and ketones, wherein the content of acetic acid in the organic component is the largest, the organic phenolic resin has high activity, the ortho-substituted phenol compounds have higher activity than phenol, and the pH value is 1.5-3.7. At present, wood vinegar is mainly used for pesticide additives, soil conditioners, plant growth regulators, food additives, deodorants, preservatives, medicinal raw materials and the like at home and abroad.
Although the pyroligneous liquor is also used as a disinfectant at present, the pyroligneous liquor is combined with other chemical substances or other effective disinfecting components for application, so that the disinfecting effect of the pyroligneous liquor is not maximized, and the environment is excessively disinfected by using other chemical substances, which is unfavorable for the environment of livestock and poultry farms.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a disinfectant containing pyroligneous, which can be used for disinfecting the environment of livestock and poultry farms and the surfaces of articles, and is a novel disinfectant, which consists of pyroligneous, an antioxidant, a metal ion complexing agent and a surfactant, the pyroligneous prepared by adopting a specific process has the best quality of effective components for disinfection, and further has the best disinfection effect by the synergistic effect of the antioxidant, the metal ion complexing agent and the surfactant, and the adverse effect on the environment of the livestock and poultry farms caused by over-disinfection of the environment is avoided.
The specific technical scheme of the invention is realized as follows:
a disinfectant containing wood vinegar comprises wood vinegar, an antioxidant, a metal ion complexing agent and a surfactant, and comprises the following components in parts by weight: 74.7 to 84.895 parts of wood vinegar, 0.1 to 0.2 part of antioxidant, 0.005 to 0.1 part of metal ion complexing agent and 15 to 25 parts of surfactant.
The preparation method of the wood vinegar comprises the following steps:
(1) condensing and separating a steam gas mixture derived by dry distillation and pyrolysis of broad-leaved tree branches and trunks growing at 46-degree north latitude for more than 100 years to obtain a red brown liquid;
(2) pouring the reddish brown liquid obtained in the step (1) into a container, standing, aging and separating for 3-5 years, and collecting an intermediate clear part to obtain crude wood vinegar;
(3) and (3) distilling the crude pyroligneous liquor obtained in the step (2) again at normal pressure, and collecting fractions at the temperature of 98-103 ℃, namely the required pyroligneous liquor.
The longer the aging time in the step (2) is, the better the quality of the pyroligneous liquor is, the aging time is preferably 5 years, and the collected is the middle clear part of the aging because the middle clear part has the best quality, the most effective components and the best disinfection effect.
When the fraction in the step (3) is collected, the fraction below 98 ℃ is unstable and contains components with low boiling points, such as methanol and formaldehyde, which are toxic to organisms; the fraction at a temperature higher than 103 deg.C contains 3, 4-benzopyrene and other high-boiling point toxic chemical components; the collected fraction is 98-103 deg.c, and the fraction in this temperature range is acetic acid or its component near the boiling point of acetic acid, and is the required effective pyroligneous liquor, and contains no toxic chemical components.
The pyroligneous liquor is separated into harmful ingredients through a specific process, and nontoxic active ingredients are reserved, so that the disinfection effect of the pyroligneous liquor is best, and the adverse effect on the environment of the livestock and poultry farm caused by excessive disinfection on the environment is avoided.
The pyroligneous liquor is a red brown liquid obtained by condensing and separating a steam gas mixture derived by dry distillation pyrolysis of broad-leaved tree branches and trunks growing at 46-degree growth period of north latitude for more than 100 years, wherein the broad-leaved tree branches and trunks are preferably branches and woods of oak and birch, the problem of waste utilization is solved, and a new way is provided for development of biomass energy. The obtained wood vinegar is a natural mixture with very complex components, the main components of the wood vinegar are organic acid, phenols, alcohols and ketones, the content of acetic acid is maximum, the wood vinegar has high activity, ortho-substituted phenol compounds have higher activity than phenol, and the pH value of the obtained wood vinegar is 1.5-3.7, preferably 1.9-2.5, and more preferably 2.5. This is because it is possible to maximize the disinfection effect of the pyroligneous acid solution and to avoid excessive disinfection of the environment.
The antioxidant is selected from one or more of sodium bisulfite, sodium metabisulfite, tert-butyl p-hydroxyanisole (BHA), 2, 6-di-tert-butyl p-cresol (BHT), Propyl Gallate (PG) and tert-butyl hydroquinone (TBHQ); sodium bisulfite is preferred.
The metal ion complexing agent is selected from one or more of Ethylene Diamine Tetraacetic Acid (EDTA), disodium ethylene diamine tetraacetic acid (EDTA-2Na), dipotassium ethylene diamine tetraacetic acid (EDTA-2K), citric acid and tartaric acid; preferably ethylenediaminetetraacetic acid (EDTA).
The surfactant is selected from one or more of benzalkonium bromide, benzalkonium chloride, sodium dodecyl benzene sulfate, sodium hexadecyl sulfate and sodium octadecyl sulfate; benzalkonium bromide is preferred.
A disinfectant containing wood vinegar is prepared by adding wood vinegar, antioxidant, metal ion complexing agent, and surfactant into acid-resistant container according to the above formula ratio, and mixing.
The disinfectant is applied to the environmental disinfection and the surface disinfection of articles in livestock and poultry farms.
The main components of the wood vinegar liquid are organic acid, phenols, ketones, alcohols, aldehydes and the like, and are main components of the disinfectant, the antioxidant mainly plays a role in preventing the wood vinegar liquid from being oxidized, the metal ion complexing agent plays a role in assisting the antioxidant, and the surfactant is beneficial to preparing a homogeneous solution and can exert the disinfection effect to the maximum. The components interact with each other, so that the disinfectant has stable property and is nonvolatile, which is beneficial to environmental disinfection and surface disinfection of articles and can not cause excessive disinfection.
The invention has the beneficial effects that:
(1) the branches of the broad-leaved trees growing at the 46-degree growth period of the north latitude for more than 100 years are preferably branches and woods of oak and birch, so that the problem of waste utilization is solved, the environmental pollution is avoided, the economic benefit is improved, and a new way is provided for the development of biomass energy;
(2) when preparing the pyroligneous liquor, the intermediate clarified part of the aging is collected in the step (2) because the intermediate clarified part has the best quality, the most effective components and the best disinfection effect;
(3) harmful ingredients are separated by a specific process, and nontoxic effective ingredients are reserved, so that the disinfection effect of the pyroligneous liquor is the best, and adverse effects on the environment of the livestock and poultry farm caused by excessive disinfection on the environment are avoided;
(4) the disinfectant prepared by taking the wood vinegar as the raw material and cooperating with the antioxidant, the metal ion complexing agent and the surfactant has stable property and is non-volatile, so that the effective disinfection effect of the wood vinegar is exerted to the maximum, and the side effect of harmful ingredients on the skin of the pet can be avoided.
Detailed Description
The preparation method of the wood vinegar comprises the following steps:
(1) condensing and separating a steam gas mixture derived by dry distillation and pyrolysis of broad-leaved tree branches and trunks growing at 46-degree north latitude for more than 100 years to obtain a red brown liquid;
(2) pouring the reddish brown liquid obtained in the step (1) into a container, standing, aging and separating for 3-5 years, and collecting an intermediate clear part to obtain crude wood vinegar;
(3) and (3) distilling the crude pyroligneous liquor obtained in the step (2) again at normal pressure, and collecting fractions at the temperature of 98-103 ℃, namely the required pyroligneous liquor.
When the fraction in the step (3) is collected, the fraction below 98 ℃ is unstable and contains components with low boiling points, such as methanol and formaldehyde, which are toxic to organisms; the fraction at a temperature higher than 103 deg.C contains 3, 4-benzopyrene and other high-boiling point toxic chemical components; the collected fraction is 98-103 deg.c, and the fraction in this temperature range is acetic acid or its component near the boiling point of acetic acid, and is the required effective pyroligneous liquor, and contains no toxic chemical components.
Example 1
A disinfectant containing wood vinegar comprises wood vinegar, an antioxidant, a metal ion complexing agent and a surfactant, and comprises the following components in parts by weight: 84 parts of pyroligneous, 0.2 part of antioxidant sodium bisulfite, 0.1 part of metal ion complexing agent Ethylene Diamine Tetraacetic Acid (EDTA), and 15.7 parts of surfactant benzalkonium bromide. The pH of the wood vinegar solution is 1.9
The preparation method of the disinfectant containing the pyroligneous liquor comprises the steps of adding 84 parts of pyroligneous liquor, 0.2 part of antioxidant sodium bisulfite, 0.1 part of metal ion complexing agent ethylenediaminetetraacetic acid (EDTA) and 15.7 parts of surfactant benzalkonium bromide into an acid-resistant container according to the formula proportion, and mixing to obtain the disinfectant.
The disinfectant is applied to the environmental disinfection and the surface disinfection of articles in livestock and poultry farms.
Example 2
A disinfectant containing wood vinegar comprises wood vinegar, an antioxidant, a metal ion complexing agent and a surfactant, and comprises the following components in parts by weight: 80 parts of pyroligneous liquor, 0.2 part of antioxidant sodium bisulfite, 0.1 part of metal ion complexing agent Ethylene Diamine Tetraacetic Acid (EDTA) and 19.7 parts of surfactant benzalkonium bromide. The pH of the wood vinegar liquid is 1.5.
The preparation method of the disinfectant containing the pyroligneous liquor comprises the steps of adding 80 parts of pyroligneous liquor, 0.2 part of antioxidant sodium bisulfite, 0.1 part of metal ion complexing agent ethylenediaminetetraacetic acid (EDTA) and 19.7 parts of surfactant benzalkonium bromide into an acid-resistant container according to the formula proportion, and mixing to obtain the disinfectant.
The disinfectant is applied to the environmental disinfection and the surface disinfection of articles in livestock and poultry farms.
Example 3
A disinfectant containing wood vinegar comprises wood vinegar, an antioxidant, a metal ion complexing agent and a surfactant, and comprises the following components in parts by weight: 78 parts of pyroligneous, 0.1 part of antioxidant sodium bisulfite, 0.1 part of metal ion complexing agent ethylenediamine tetraacetic acid (EDTA) and 21.8 parts of surfactant benzalkonium bromide. The pH of the wood vinegar liquid is 1.6.
The preparation method of the disinfectant containing the pyroligneous liquor comprises the steps of adding 78 parts of pyroligneous liquor, 0.1 part of antioxidant sodium bisulfite, 0.1 part of metal ion complexing agent ethylenediaminetetraacetic acid (EDTA) and 21.8 parts of surfactant benzalkonium bromide into an acid-resistant container according to the formula proportion, and mixing to obtain the disinfectant.
The disinfectant is applied to the environmental disinfection and the surface disinfection of articles in livestock and poultry farms.
The above examples 1-3 are preferred examples and the formulations of the disinfectants described in the other examples 4-7 are shown in table 1 below:
table 1 formulations of disinfectant containing wood vinegar as described in examples 1-7
The disinfectant prepared by the formula described in the above examples 1-7 was used for the disinfection effect test:
1 materials and methods
1.1 Chicken farm conditions 2 chicken houses were selected from a standardized coop in Shandong Weifang City, and the chicken house conditions are shown in Table 2.
TABLE 2 brief introduction to the Chicken farm
1.2 Disinfection mode
1, chicken house: after being washed by clean water, the disinfectant is sprayed on the sterilized chicken coop at a ratio of 1: 20.
And 2, chicken houses: after being washed by clean water, 1:20 glutaraldehyde sunflower methyl ammonium bromide disinfectant is sprayed on the sterilized chicken coop.
And 1.3, sampling after the chicken manure is cleaned and washed at the sampling time as sample data before disinfection, and sampling before the chicken is put on after disinfection as data after disinfection for sampling detection.
1.4 detection of content of airborne aerobic bacteria, escherichia coli, staphylococcus aureus and fungi adopts an FA-1 six-level sieve mesh impact type air microorganism sampler which is arranged at the center of a diagonal line in a house, the height is 1.2m, the air flow speed is 28.3 L.min < -1 >, 5% defibrinated sheep blood agar culture medium, eosin methylene blue culture medium, Baird-parker culture medium and Bengal Red (RBM) culture medium are respectively adopted as sampling mediums, the driving time is 5min, and the colony number on each plate is preferably below 250. Placing the culture medium in a 37 ℃ incubator after sampling, carrying out colony counting after 5% defibrinated sheep blood agar culture medium is cultured for 24 hours, selecting typical colonies for counting after eosin methylene blue culture medium is cultured for 24 hours, selecting typical colonies for counting after Baird-parker culture medium is cultured for 48 hours, carrying out colony counting after Bengal red culture medium is placed in a 28 ℃ incubator and cultured for 3-7 days according to a formula: number of microorganisms in air (CFU)/m3Average colony count of all plates/[ sampling time (min) X28.3(L · min)-1)]And X1000 is calculated.
1.5 statistical analysis data analysis was performed using SPSS Statistics 19.0 statistical analysis software, multiple comparisons were performed using the LSD method, and test data were compared using mean. + -. standard deviation and significance.
2 results of the test
2.1 detection of environment aerobes, escherichia coli, staphylococcus aureus and gas-borne fungi in the chicken house, as can be seen from table 3, the concentration of the aerobes in the 1 st chicken house after disinfection is significantly lower than that in the 2 nd chicken house (p < 0.05); the concentration of airborne escherichia coli in the 1 st chicken house is significantly lower than that in the 2 nd chicken house (p < 0.05); wherein the differences after the disinfection of staphylococcus aureus and airborne fungi are not significant (p > 0.05). Therefore, the disinfection effect of the disinfectant is obviously better than that of a glutaraldehyde sunflower ammonium bromide disinfectant (p is less than 0.05); and the disinfectant prepared in examples 1 to 3 has a significantly better disinfecting effect than the disinfectant prepared in examples 4 to 7.
TABLE 3 concentration of aerobes, E.coli, S.aureus, airborne fungal aerosols (× 10) in henhouses before and after sterilization3CFU/m3)
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (9)
1. A disinfectant comprising pyroligneous acid, characterized in that: the wood vinegar liquid comprises wood vinegar liquid, an antioxidant, a metal ion complexing agent and a surfactant, and is prepared from the following components in parts by weight: 74.7 to 84.895 parts of wood vinegar, 0.1 to 0.2 part of antioxidant, 0.005 to 0.1 part of metal ion complexing agent and 15 to 25 parts of surfactant;
the pH value of the wood vinegar is 1.5-3.7.
2. The disinfectant containing pyroligneous acid according to claim 1, wherein: the pH value of the wood vinegar is 1.9-2.5.
3. The disinfectant containing pyroligneous acid according to claim 1, wherein: the preparation method of the wood vinegar comprises the following steps:
(1) condensing and separating a steam gas mixture derived by dry distillation and pyrolysis of broad-leaved tree branches and trunks growing at 46-degree north latitude for more than 100 years to obtain a red brown liquid;
(2) pouring the reddish brown liquid obtained in the step (1) into a container, standing, aging and separating for 3-5 years, and collecting an intermediate clear part to obtain crude wood vinegar;
(3) and (3) distilling the crude pyroligneous liquor obtained in the step (2) again at normal pressure, and collecting fractions at the temperature of 98-103 ℃, namely the required pyroligneous liquor.
4. The disinfectant containing wood vinegar as set forth in claim 3, wherein: in the step (1), the broad-leaved tree branches are twigs and woods of oak and birch.
5. The disinfectant containing pyroligneous acid according to claim 1, wherein: the antioxidant is selected from one or more of sodium bisulfite, sodium pyrosulfite, tert-butyl p-hydroxyanisole (BHA), 2, 6-di-tert-butyl p-cresol (BHT), Propyl Gallate (PG) and tert-butyl hydroquinone (TBHQ).
6. The disinfectant containing pyroligneous acid according to claim 1, wherein: the metal ion complexing agent is one or more selected from Ethylene Diamine Tetraacetic Acid (EDTA), disodium ethylene diamine tetraacetic acid (EDTA-2Na), dipotassium ethylene diamine tetraacetic acid (EDTA-2K), citric acid and tartaric acid.
7. The disinfectant containing pyroligneous acid according to claim 1, wherein: the surfactant is one or more selected from benzalkonium bromide, benzalkonium chloride, sodium dodecylbenzene sulfate, sodium hexadecyl sulfate and sodium octadecyl sulfate.
8. A method of preparing a disinfectant containing pyroligneous acid according to claim 1, wherein: adding wood vinegar, an antioxidant, a metal ion complexing agent and a surfactant into an acid-resistant container according to the formula proportion, and mixing to obtain the disinfectant.
9. Use of a disinfectant comprising pyroligneous acid according to claim 1, wherein: the disinfectant is applied to the environmental disinfection and the surface disinfection of articles in livestock and poultry farms.
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| CN202011287565.4A CN112369432A (en) | 2020-11-17 | 2020-11-17 | Disinfectant containing wood vinegar and preparation method and application thereof |
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| CN202011287565.4A CN112369432A (en) | 2020-11-17 | 2020-11-17 | Disinfectant containing wood vinegar and preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117016678A (en) * | 2023-09-28 | 2023-11-10 | 山东夙沙高科生物有限公司 | Wood vinegar feed additive and preparation method thereof |
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2020
- 2020-11-17 CN CN202011287565.4A patent/CN112369432A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117016678A (en) * | 2023-09-28 | 2023-11-10 | 山东夙沙高科生物有限公司 | Wood vinegar feed additive and preparation method thereof |
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