CN105211064A - A kind of termite bait formulation containing ethyl pleocidin - Google Patents
A kind of termite bait formulation containing ethyl pleocidin Download PDFInfo
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- CN105211064A CN105211064A CN201510707823.2A CN201510707823A CN105211064A CN 105211064 A CN105211064 A CN 105211064A CN 201510707823 A CN201510707823 A CN 201510707823A CN 105211064 A CN105211064 A CN 105211064A
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- ethyl pleocidin
- termite
- bait formulation
- trehalose
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Abstract
The invention discloses a kind of termite bait formulation containing ethyl pleocidin.By mass fraction, comprise ethyl pleocidin 0.001% ~ 0.01%, grand edge eucalyptus barks powder 15% ~ 35%, pine sawdust 15% ~ 35%, trehalose 4.99% ~ 4.999% and flour 40% ~ 45%.Termite bait formulation containing ethyl pleocidin of the present invention, its active ingredient ethyl pleocidin belongs to biological insecticides, easily degrade in natural environment, to mammal, aquatic animal and birds safety, and having good control efficiency to termite, is the environment amenable control termites bait formulation of a kind of highly effective and safe.
Description
Technical field:
The invention belongs to termite control field, be specifically related to a kind of termite bait formulation containing ethyl pleocidin.
Background technology:
Termite belongs to the insect of Isoptera, the international insect physiological ecological research center (ICTPE) of Zeng Bei is classified as one of worldwide five major pests, its harm almost relates to national economy every field, especially serious to harm such as building construction, mountain forest orchard, reservoir dam, communication apparatus.
In the past few decades, the major pharmaceutical agent of domestic control termites is mirex, but mirex is organochlorine class medicament, chemical constitution is very stable in the environment, while effective control termites, certainly will pollute soil and water resource, enter human body finally by food chain, potential harm is brought to human body health.Along with human society is to the strengthening day by day of environmental protection consciousness, international community to hazardous chemical particularly agricultural chemicals propose more and more higher requirement.More than 90 countries of China are comprised in the United Nations's meeting that Sweden Si Degeer holds in May 23 calendar year 2001, endorsed " the Si Degeer pact about persistence organic pollutant " (POPs pact), POPs pact determines forbid in worldwide or strictly limit the use of 12 kinds of organic pollutants, wherein to 7 kinds of organic insecticides such as the current the most effective medicine mirex for control termites and ant and Nirans, emphasize to stop producing and using.
Exploitation filters out efficiently, safely, meet the termiticides of environmental requirement, has become the problem that China's termite control one is extremely to be solved.To so far, have no the report of ethyl pleocidin for control termites.
Ethyl pleocidin (Spinetoram) is a kind of biological insecticides produced from actinomycetes thorn saccharopolyspora strain (Saccharopolysporaspinosa) fermentation, its former the effective elements of the medicine is the mixture (ratio is 3: 1) of ethyl pleocidin-J and ethyl pleocidin-L, its mechanism of action acts on nAChR and gamma-aminobutyric acid receptor in insect nerve, cause the signal transmission reaction of polypide to excitability or inhibition insensitive, affect normal nervous activity, until dead.Ethyl pleocidin has stomach toxicity and action of contace poison.
Summary of the invention:
The object of this invention is to provide a kind of efficient, safe termite bait formulation containing ethyl pleocidin.
Because termite is social insect, when adopting bait method control termites, first be chronic medicament is mixed in the food of termite eating make poison bait, then poison bait is discharged at termite activity place, allow worker ant freely take food and poisonous baits taken back in nest, the medicament in bait mainly through colony Nutrition Attitude and foster behavior such as trophallaxis, ornamenting etc. to spread in colony.Therefore, desirable termite poison bait, its medicament should be efficient, chronic and to people and animals' low toxicity stomach poison, should without anti-repelling properties and antifeedant to termite, and medicament also should be able to pass to non-individuality of looking for food by individuality of looking for food simultaneously.
The former medicine of ethyl pleocidin is with musty and semen armeniacae amarae taste, the present inventor is found by laboratory test, the ethyl pleocidin bait formulation not adding trehalose has certain Antifeedant Effects to termite, but after with the addition of appropriate trehalose, musty and the semen armeniacae amarae taste of ethyl pleocidin are reduced, not only this termite bait formulation containing ethyl pleocidin is to termite without antifeedant reaction, also show bait formulation and has obvious attracting action to termite.
Described trehalose is with α by two glucose molecules; α; 1; the nonreducing sugar that 1-glycosidic bond is formed; self property is highly stable; there is unique biological property, protective effect to biological anti-dehydrating, anti-freezing protective effect and anti-height and ooze protective effect, impart simultaneously and prevent age of starch, prevent protein denaturation, suppress lipid material to become sour, reduce astringent taste and bitter taste, rectification taste and the effect of rectification smell.And occurring in nature is as other carbohydrate such as sucrose, glucose; all do not possess, to various bioactivators, there is magical this function of protective effect; the functional characteristic of this uniqueness, can be used as and prevent foodstuff deteriorate, expanded the function of trehalose as natural edible sweet taste sugar.
Containing starchy material flour in termite bait formulation, therefore bait formulation runs into moist environment in the wild and is easy to mouldy after throwing in.And the poison bait termite gone mouldy is not liked taking food, giving full play to of poison bait drug effect certainly will be affected.But add inappropriate mould inhibitor and may affect bait formulation to termite phagostimulating effect.
The present inventor finds, trehalose not only as attractant and subduction odorant agent, also serves certain fresh-keeping anti-mildew effect in this patent formula.Test at the identical temperature in outdoor and wet environment and show, the termite bait formulation containing ethyl pleocidin of interpolation trehalose is about postponed than the termite bait formulation containing ethyl pleocidin not adding trehalose and being gone bad for 3 days, this plays important effect for increasing the food ingestion of termite to bait formulation, improves preventive effect.
Termite bait formulation containing ethyl pleocidin of the present invention, it is characterized in that, by mass fraction, comprise ethyl pleocidin 0.001% ~ 0.01%, grand edge eucalyptus (EucalyptusexsertaF.V.Muell.) bark powder 15% ~ 35%, pine sawdust 15% ~ 35%, trehalose 4.99% ~ 4.999% and flour 40% ~ 45%.
Termite bait formulation containing ethyl pleocidin of the present invention, its active ingredient is ethyl pleocidin, belong to biological insecticides, easily degrade in natural environment, to mammal, aquatic animal and birds safety, and having good control efficiency to termite, is the environment amenable control termites biological insecticides of a kind of highly effective and safe.
Embodiment:
Following examples further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1:
This example containing 0.001% ethyl pleocidin termite bait formulation of trehalose, by mass fraction, does not comprise ethyl pleocidin 0.001%, grand edge eucalyptus barks powder 20%, pine sawdust 35%, and flour 44.999%.By above-mentioned composition and content, by ethyl pleocidin, grand edge eucalyptus barks powder, adds appropriate water after pine sawdust and flour mix, the cuboid of 6cm × 2cm × 0.6cm made by recycling mould, obtains not containing 0.001% ethyl pleocidin termite bait formulation of trehalose after oven dry.
This example, containing 0.001% ethyl pleocidin termite bait formulation of trehalose, by mass fraction, comprises ethyl pleocidin 0.001%, grand edge eucalyptus barks powder 20%, pine sawdust 35%, trehalose 4.999% and flour 40%.By above-mentioned composition and content, by ethyl pleocidin, grand edge eucalyptus barks powder, pine sawdust, add appropriate water after trehalose and flour mix, the cuboid of 6cm × 2cm × 0.6cm made by recycling mould, obtains the 0.001% ethyl pleocidin termite bait formulation containing trehalose after oven dry.
This example does not contain the contrast termite bait bar of ethyl pleocidin yet containing trehalose, by mass fraction, comprise, grand edge eucalyptus barks powder 20%, pine sawdust 35% and flour 45%.By above-mentioned composition and content, by grand edge eucalyptus barks powder, add appropriate water after pine sawdust and flour mix, the cuboid of 6cm × 2cm × 0.6cm made by recycling mould, obtains contrast termite bait bar after oven dry.
The moistening fine sand (accounting for 1/2nd of culture dish floor space) of the thick 1cm of side layer overlay in the culture dish that diameter is 15cm, opposite side drops into the 0.001% ethyl pleocidin termite bait formulation one containing trehalose, 50 Workers of Coptotermes formosanus Shiraki worker ants are put into cultivation from fine sand side, when noting dropping into, termite is avoided directly to contact with test bait formulation.Another group drops into not containing the 0.001% ethyl pleocidin termite bait formulation one of trehalose, and control group drops into and do not contain ethyl pleocidin also not containing the contrast termite bait bar one of trehalose.If 3 repetitions.Every day time recording termite the situation that takes food and termite mortality number.The results are shown in Table 1.
The comparative trial of table 1 three kinds of different formulations bait bars
In table, data are mean value ± standard error, and in same column, different letter has significant difference, P < 0.05
Result of the test: the 0.001% ethyl pleocidin termite bait formulation not containing trehalose has certain Antifeedant Effects to Workers of Coptotermes formosanus Shiraki, but the 0.001% ethyl pleocidin termite bait formulation that with the addition of 4.999% trehalose without antifeedant, and has good food calling and toxic effect to Workers of Coptotermes formosanus Shiraki.
Embodiment 2
This example containing 0.003% ethyl pleocidin termite bait formulation of trehalose, by mass fraction, does not comprise ethyl pleocidin 0.003%, grand edge eucalyptus barks powder 20%, pine sawdust 35%, and flour 44.997%.By above-mentioned composition and content, by ethyl pleocidin, grand edge eucalyptus barks powder, adds appropriate water after pine sawdust and flour mix, the cuboid of 6cm × 2cm × 0.6cm made by recycling mould, obtains not containing 0.003% ethyl pleocidin termite bait formulation of trehalose after oven dry.
This example, containing 0.003% ethyl pleocidin termite bait formulation of trehalose, by mass fraction, comprises ethyl pleocidin 0.003%, grand edge eucalyptus barks powder 20%, pine sawdust 35%, trehalose 4.997% and flour 40%.By above-mentioned composition and content, by ethyl pleocidin, grand edge eucalyptus barks powder, pine sawdust, add appropriate water after trehalose and flour mix, the cuboid of 6cm × 2cm × 0.6cm made by recycling mould, obtains the 0.003% ethyl pleocidin termite bait formulation containing trehalose after oven dry.
On the meadow not having termite, by not containing trehalose 0.003% ethyl pleocidin termite bait formulation and containing 0.003% ethyl pleocidin termite bait formulation of trehalose each 1 be put into thick grass under, each bait formulation directly contacts with earth, and on each bait bar upper cover leaf or hay, establish 5 repetitions altogether, every day observed and recorded weather condition and bait formulation whether rotten mouldy.
Result of the test: it does not rain at this duration of test continuous 8, the 0.003% ethyl pleocidin termite bait formulation not containing trehalose all occurred rotten mouldy at the 5th day, and all occurred rotten mouldy containing 0.003% ethyl pleocidin termite bait of trehalose to the 8th talent.
Embodiment 3:
The termite bait formulation containing ethyl pleocidin of the present embodiment, by mass fraction, comprises ethyl pleocidin 0.002%, grand edge eucalyptus barks powder 35%, pine sawdust 15%, trehalose 4.998% and flour 45%.By above-mentioned composition and content, by ethyl pleocidin, grand edge eucalyptus barks powder, pine sawdust, add appropriate water after trehalose and flour mix, the cuboid of 6cm × 2cm × 0.6cm made by recycling mould, obtains the termite bait formulation containing ethyl pleocidin after oven dry.
Mud is by mud line dispenser control dam termite: check on dam slope and find that fresh mud is by after mud line, by being placed on mud that termite constructs gently containing the termite bait formulation of ethyl pleocidin by above mud line or side, then covers with hay or leaf.Reexamine afterwards week about, dispenser, continuous dispenser 3 times, check after one month mud by mud line reduce more than 95%.
Embodiment 4:
The termite bait formulation containing ethyl pleocidin of the present embodiment, by mass fraction, comprises ethyl pleocidin 0.005%, grand edge eucalyptus barks powder 35%, pine sawdust 20%, trehalose 4.995% and flour 40%.By above-mentioned composition and content, by ethyl pleocidin, grand edge eucalyptus barks powder, pine sawdust, add appropriate water after trehalose and flour mix, mixture is made the cuboid that specification is 6cm × 2cm × 0.6cm by recycling mould, obtains the termite bait formulation containing ethyl pleocidin after oven dry.
Swarming hole dispenser control dam termite: in the dam termite swarming phase in 4 ~ May, after dam slope finds termite swarming hole, each swarming hole is all with the earth of scuppit shovel perforate mouth projection, termite bait formulation containing ethyl pleocidin is filled in after in hole again with shoveling the earth sealing of opening, reexamine after one week, find that there is the dispenser again of swarming hole.Checking around swarming hole June to October and find the dead nest indicator bacteria of termite---Xylaria sp. fungus, illustrates this nest termite dead.
Embodiment 5:
The termite bait formulation containing ethyl pleocidin of the present embodiment, by mass fraction, comprises ethyl pleocidin 0.01%, grand edge eucalyptus barks powder 25%, pine sawdust 25%, trehalose 4.99% and flour 45%.By above-mentioned composition and content, by ethyl pleocidin, grand edge eucalyptus barks powder, pine sawdust, add appropriate water after trehalose and flour mix, mixture is made the cuboid that specification is 6cm × 2cm × 0.6cm by recycling mould, obtains the termite bait formulation containing ethyl pleocidin after oven dry.
Control forest termite: find the Ni Beihuoyi road of termite on trunk after, the termite bait formulation containing ethyl pleocidin is against Ni Beihuoyi road, ties up with rope or follow closely fixing, reexamine afterwards week about, tonic, continuous dispenser 3 times, checked after 1 month, and the rate of being injured of forest reduces more than 95%.
Embodiment 6:
The termite bait formulation containing ethyl pleocidin of the present embodiment, by mass fraction, comprises ethyl pleocidin 0.001%, grand edge eucalyptus barks powder 15%, pine sawdust 35%, trehalose 4.999% and flour 45%.By above-mentioned composition and content, by ethyl pleocidin, grand edge eucalyptus barks powder, pine sawdust, add appropriate water after trehalose and flour mix, mixture is made the cuboid that specification is 6cm × 2cm × 0.6cm by recycling mould, obtains the termite bait formulation containing ethyl pleocidin after oven dry.
Control building termite: check in building find the termite activity such as ant road, swarming hole expose feature after, be close to these characteristic bodies and execute the termite bait formulation put containing ethyl pleocidin, fix with adhesive tape if needed; Check that bait formulation is filled in timber structure inside after having termite damage can pry open a seam with screwdriver by timber structure inside, check after the week, find to expose feature dispenser again, after one month, check that the characteristic sum harm that exposes of termite activity disappears.
Claims (1)
1. the termite bait formulation containing ethyl pleocidin, it is characterized in that, by mass fraction, comprise ethyl pleocidin 0.001% ~ 0.01%, grand edge eucalyptus barks powder 15% ~ 35%, pine sawdust 15% ~ 35%, trehalose 4.99% ~ 4.999% and flour 40% ~ 45%.
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| CN201510707823.2A CN105211064A (en) | 2015-10-26 | 2015-10-26 | A kind of termite bait formulation containing ethyl pleocidin |
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| CN201510707823.2A CN105211064A (en) | 2015-10-26 | 2015-10-26 | A kind of termite bait formulation containing ethyl pleocidin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106376555A (en) * | 2016-08-31 | 2017-02-08 | 广东中迅农科股份有限公司 | Application technology of trehalose in insecticidal micro-emulsion |
| CN107047623A (en) * | 2017-03-01 | 2017-08-18 | 华中农业大学 | A kind of casing bait formulation of energy trapping and killing termites |
| CN108925575A (en) * | 2018-08-16 | 2018-12-04 | 广州粤昆源生物科技发展有限公司 | A kind of medicament of prevention and treatment termite and red fire ant with security role |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106376555A (en) * | 2016-08-31 | 2017-02-08 | 广东中迅农科股份有限公司 | Application technology of trehalose in insecticidal micro-emulsion |
| CN107047623A (en) * | 2017-03-01 | 2017-08-18 | 华中农业大学 | A kind of casing bait formulation of energy trapping and killing termites |
| CN107047623B (en) * | 2017-03-01 | 2020-04-07 | 华中农业大学 | Casing bait capable of trapping and killing termites |
| CN108925575A (en) * | 2018-08-16 | 2018-12-04 | 广州粤昆源生物科技发展有限公司 | A kind of medicament of prevention and treatment termite and red fire ant with security role |
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Address after: 510260 Xingang West Road, Guangdong, Guangzhou, No. 105, No. Applicant after: Guangdong Institute of biological resources application Address before: 510260 Xingang West Road, Guangdong, Guangzhou, No. 105, No. Applicant before: Guangdong Entomological Institute |
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