CN105210878A - A kind of Rapid Propagation of Pinellia ternate method - Google Patents
A kind of Rapid Propagation of Pinellia ternate method Download PDFInfo
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- CN105210878A CN105210878A CN201510683696.7A CN201510683696A CN105210878A CN 105210878 A CN105210878 A CN 105210878A CN 201510683696 A CN201510683696 A CN 201510683696A CN 105210878 A CN105210878 A CN 105210878A
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- pinellia
- pinellia ternate
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Abstract
The present invention relates to a kind of Rapid Propagation of Pinellia ternate method, comprise the steps: to get half summer sowing ball, the induced growth of indefinite bud is cultivated, and obtains the tissue block with indefinite bud clump, and root induction is cultivated and obtained tuber of pinellia seedling.Method regeneration efficiency of the present invention increases, and bud ratio is about 100%, and pollution rate reduces, and there will not be situation about repeatedly polluting in incubation, can improve inductivity by regulating culture medium condition and shorten the regeneration period.
Description
Technical field
The present invention relates to biological medicinal material tuber of pinellia seed growing expanding species method, this technology directly applies to biological medicinal material and breeds expanding species, particularly relates to a kind of Rapid Propagation of Pinellia ternate method.
Background technology
The tuber of pinellia (formal name used at school: Pinelliaternata), has another name called ground literary composition, keeps field etc., belong to Arales.Be distributed widely in Yangtze River in China basin and the area such as northeast, North China.Medicinal plant, has eliminating dampness and eliminating phlegm, stopping nausea and vomiting by lowering the adverse flow of QI, and raw with the furuncle effect that disappears, animal doctor is in order to control synanche Huang.Also distribution is had, height above sea level about 3000m in Tibet.
Tuber of pinellia scale is the group training material of the Fast-propagation commonly used the most.Research shows, leaf blade of Pinellia and ovary also can regenerate formation seedling.Although with bulb be explant carry out regenerating draw materials relatively convenient, but because bulb carries disease germs seriously, usually cause sterilization not thorough, in incubation, contamination phenomenon is very serious repeatedly, increase workload, and be that explant regeneration efficiency is not very good with scale.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Rapid Propagation of Pinellia ternate method.Method regeneration efficiency of the present invention increases, and pollution rate reduces, and by regulating culture medium condition to improve inductivity, shorten the regeneration period, this regenerating system is applicable to multiple tuber of pinellia kind.
The technical scheme that the present invention solves the problems of the technologies described above is as follows: a kind of Rapid Propagation of Pinellia ternate method, comprises the steps:
1) choose healthy seamless tuber of pinellia plant, intercept its petiole part stand-by;
2) by step 1) in described petiole put into 70% ~ 90% soak 40 ~ 50S, rinse well with clear water after taking-up;
3) by step 2) in the petiole that the obtains liquor potassic permanganate of putting into 5% ~ 7% soak 0.5 ~ 1min, rinse well with clear water after taking-up;
4) by step 3) the petiole body that obtains, put into induced growth MS solid culture medium carrying out indefinite bud and cultivate, obtain the tissue block with indefinite bud clump;
5) by step 4) tissue block that obtains makes a vertical point tissue block, and bud elongation medium is carried out bud and extends and cultivate;
6) until step 4) Elongation of adventitious bud to 5 ~ 8cm of obtaining time, put into root media after carrying out cutting and carry out root induction cultivation, obtain tuber of pinellia seedling.
The invention has the beneficial effects as follows: method regeneration efficiency of the present invention increases, bud ratio is about 100%, and pollution rate reduces, and there will not be situation about repeatedly polluting in incubation, can improve inductivity by regulating culture medium condition and shorten the regeneration period.
On the basis of technique scheme, the present invention can also do following improvement.
Further, described MS solid culture medium is MS+BA1.8 ~ 2.2mg/L+NAA0.8 ~ 1.2mg/L, pH5.8, sucrose 28 ~ 32g/L, agar 4 ~ 6g/L.
Further, described bud elongation medium is MS+BA0.3 ~ 0.7mg/L+NAA0.8 ~ 1.2mg/L, pH5.8, sucrose 28 ~ 32g/L, agar 4 ~ 6g/L.
Further, described root media is MS+BA0.3 ~ 0.7mg/L+NAA0.8 ~ 1.2mg/L, pH5.8, sucrose 28 ~ 32g/L, agar 4 ~ 6g/L.
Further, step 2) in, the condition that described induced growth is cultivated is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
Further, step 3) in, described bud extends the condition of cultivating and is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
Further, step 4) in, the condition that described root induction is cultivated is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
Embodiment
Be described principle of the present invention and feature below in conjunction with embodiment, example, only for explaining the present invention, is not intended to limit scope of the present invention.
Embodiment one
A kind of Rapid Propagation of Pinellia ternate method, comprises the steps:
1) choose healthy seamless tuber of pinellia plant, intercept its petiole part stand-by;
2) by step 1) in described petiole put into 70% immersion 40S, rinse well with clear water after taking-up;
3) by step 2) in the petiole that the obtains liquor potassic permanganate of putting into 5% soak 0.5min, rinse well with clear water after taking-up;
4) by step 3) the petiole body that obtains, put into induced growth MS solid culture medium carrying out indefinite bud and cultivate, obtain the tissue block with indefinite bud clump;
5) by step 4) tissue block that obtains makes a vertical point tissue block, and bud elongation medium is carried out bud and extends and cultivate;
6) until step 4) Elongation of adventitious bud that obtains to 5cm time, put into root media after carrying out cutting and carry out root induction cultivation, obtain tuber of pinellia seedling.
Described MS solid culture medium is MS+BA1.8mg/L+NAA0.8mg/L, pH5.8, sucrose 28g/L, agar 4g/L.
Described bud elongation medium is MS+BA0.3mg/L+NAA0.8mg/L, pH5.8, sucrose 28g/L, agar 4g/L.
Described root media is MS+BA0.3mg/L+NAA0.8mg/L, pH5.8, sucrose 28g/L, agar 4g/L.
Step 2) in, the condition that described induced growth is cultivated is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
Step 3) in, described bud extends the condition of cultivating and is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
Step 4) in, the condition that described root induction is cultivated is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
Embodiment two
A kind of Rapid Propagation of Pinellia ternate method, comprises the steps:
1) choose healthy seamless tuber of pinellia plant, intercept its petiole part stand-by;
2) by step 1) in described petiole put into 80% immersion 45S, rinse well with clear water after taking-up;
3) by step 2) in the petiole that the obtains liquor potassic permanganate of putting into 6% soak 0.8min, rinse well with clear water after taking-up;
4) by step 3) the petiole body that obtains, put into induced growth MS solid culture medium carrying out indefinite bud and cultivate, obtain the tissue block with indefinite bud clump;
5) by step 4) tissue block that obtains makes a vertical point tissue block, and bud elongation medium is carried out bud and extends and cultivate;
6) until step 4) Elongation of adventitious bud that obtains to 7cm time, put into root media after carrying out cutting and carry out root induction cultivation, obtain tuber of pinellia seedling.
Described MS solid culture medium is MS+BA2.2mg/L+NAA1.2mg/L, pH5.8, sucrose 32g/L, agar 6g/L.
Described bud elongation medium is MS+BA0.7mg/L+NAA1.2mg/L, pH5.8, sucrose 32g/L, agar 6g/L.
Described root media is MS+BA0.7mg/L+NAA1.2mg/L, pH5.8, sucrose 32g/L, agar 6g/L.
Step 2) in, the condition that described induced growth is cultivated is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
Step 3) in, described bud extends the condition of cultivating and is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
Step 4) in, the condition that described root induction is cultivated is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
Embodiment three
A kind of Rapid Propagation of Pinellia ternate method, comprises the steps:
1) choose healthy seamless tuber of pinellia plant, intercept its petiole part stand-by;
2) by step 1) in described petiole put into 90% immersion 50S, rinse well with clear water after taking-up;
3) by step 2) in the petiole that the obtains liquor potassic permanganate of putting into 7% soak 1min, rinse well with clear water after taking-up;
4) by step 3) the petiole body that obtains, put into induced growth MS solid culture medium carrying out indefinite bud and cultivate, obtain the tissue block with indefinite bud clump;
5) by step 4) tissue block that obtains makes a vertical point tissue block, and bud elongation medium is carried out bud and extends and cultivate;
6) until step 4) Elongation of adventitious bud that obtains to 8cm time, put into root media after carrying out cutting and carry out root induction cultivation, obtain tuber of pinellia seedling.
Described MS solid culture medium is MS+BA2.0mg/L+NAA1.0mg/L, pH5.8, sucrose 30g/L, agar 5g/L.
Described bud elongation medium is MS+BA0.5mg/L+NAA1.0mg/L, pH5.8, sucrose 30g/L, agar 5g/L.
Described root media is MS+BA0.5mg/L+NAA1.0mg/L, pH5.8, sucrose 30g/L, agar 5g/L.
Step 2) in, the condition that described induced growth is cultivated is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
Step 3) in, described bud extends the condition of cultivating and is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
Step 4) in, the condition that described root induction is cultivated is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. a Rapid Propagation of Pinellia ternate method, is characterized in that, comprises the steps:
1) choose healthy seamless tuber of pinellia plant, intercept its petiole part stand-by;
2) by step 1) in described petiole put into 70% ~ 90% soak 40 ~ 50S, rinse well with clear water after taking-up;
3) by step 2) in the petiole that the obtains liquor potassic permanganate of putting into 5% ~ 7% soak 0.5 ~ 1min, rinse well with clear water after taking-up;
4) by step 3) the petiole body that obtains, put into induced growth MS solid culture medium carrying out indefinite bud and cultivate, obtain the tissue block with indefinite bud clump;
5) by step 4) tissue block that obtains makes a vertical point tissue block, and bud elongation medium is carried out bud and extends and cultivate;
6) until step 4) Elongation of adventitious bud to 5 ~ 8cm of obtaining time, put into root media after carrying out cutting and carry out root induction cultivation, obtain tuber of pinellia seedling.
2. a kind of Rapid Propagation of Pinellia ternate method as claimed in claim 1, is characterized in that, described MS solid culture medium is MS+BA1.8 ~ 2.2mg/L+NAA0.8 ~ 1.2mg/L, pH5.8, sucrose 28 ~ 32g/L, agar 4 ~ 6g/L.
3. a kind of Rapid Propagation of Pinellia ternate method as claimed in claim 2, is characterized in that, described bud elongation medium is MS+BA0.3 ~ 0.7mg/L+NAA0.8 ~ 1.2mg/L, pH5.8, sucrose 28 ~ 32g/L, agar 4 ~ 6g/L.
4. a kind of Rapid Propagation of Pinellia ternate method as claimed in claim 3, is characterized in that, described root media is MS+BA0.3 ~ 0.7mg/L+NAA0.8 ~ 1.2mg/L, pH5.8, sucrose 28 ~ 32g/L, agar 4 ~ 6g/L.
5. a kind of Rapid Propagation of Pinellia ternate method as claimed in claim 4, is characterized in that, step 2) in, the condition that described induced growth is cultivated is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
6. a kind of Rapid Propagation of Pinellia ternate method as claimed in claim 5, is characterized in that, step 3) in, described bud extends the condition of cultivating and is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
7. a kind of Rapid Propagation of Pinellia ternate method as claimed in claim 6, is characterized in that, step 4) in, the condition that described root induction is cultivated is: temperature 25 DEG C, illumination: 16 photophase/7 dark phases.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105850728A (en) * | 2016-04-01 | 2016-08-17 | 荆楚理工学院 | Rapid propagation method of Pinellia ternata stems |
| CN110384043A (en) * | 2019-06-27 | 2019-10-29 | 遵义医科大学 | A kind of minimal medium, tuber of pinellia tissue culture medium (TCM) and tuber of pinellia method for tissue culture |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH0315326A (en) * | 1989-06-12 | 1991-01-23 | Nitto Denko Corp | Production of young seedling of plant of genus pinellia |
| CN102150624A (en) * | 2011-04-29 | 2011-08-17 | 南京工业大学 | Tissue culture and rapid propagation method of pinellia genus plant |
| CN102228005A (en) * | 2011-05-24 | 2011-11-02 | 汉中植物研究所 | Pinellia ternate tissue culture one-step speciation method |
| CN103931493A (en) * | 2013-01-18 | 2014-07-23 | 成都中医药大学 | Tissue culture method of pinellian ternate forming seedling through one-step culture and novel pinellia ternate medium |
-
2015
- 2015-10-20 CN CN201510683696.7A patent/CN105210878A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0315326A (en) * | 1989-06-12 | 1991-01-23 | Nitto Denko Corp | Production of young seedling of plant of genus pinellia |
| CN102150624A (en) * | 2011-04-29 | 2011-08-17 | 南京工业大学 | Tissue culture and rapid propagation method of pinellia genus plant |
| CN102228005A (en) * | 2011-05-24 | 2011-11-02 | 汉中植物研究所 | Pinellia ternate tissue culture one-step speciation method |
| CN103931493A (en) * | 2013-01-18 | 2014-07-23 | 成都中医药大学 | Tissue culture method of pinellian ternate forming seedling through one-step culture and novel pinellia ternate medium |
Non-Patent Citations (5)
| Title |
|---|
| KIM, SW等: ""Somatic embryogenesis and plant regeneration in leaf and petiole explant cultures and cell suspension cultures of Pinellia tripartita"", 《PLANT CELL TISSUE AND ORGAN CULTURE》 * |
| LIU, YH等: ""Use of protocorm-like bodies for studying alkaloid metabolism in Pinellia ternata"", 《PLANT CELL TISSUE AND ORGAN CULTURE》 * |
| 余春香: ""半夏组织培养研究"", 《贵州科学》 * |
| 宋艳梅等: ""半夏组织培养快速繁殖研究"", 《山东中医药大学学报》 * |
| 解晓红等: ""半夏脱毒技术研究及应用"", 《中药材》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105850728A (en) * | 2016-04-01 | 2016-08-17 | 荆楚理工学院 | Rapid propagation method of Pinellia ternata stems |
| CN110384043A (en) * | 2019-06-27 | 2019-10-29 | 遵义医科大学 | A kind of minimal medium, tuber of pinellia tissue culture medium (TCM) and tuber of pinellia method for tissue culture |
| CN110384043B (en) * | 2019-06-27 | 2021-09-21 | 遵义医科大学 | Basic culture medium, pinellia ternata tissue culture medium and pinellia ternata tissue culture method |
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