CN105189781B - 核苷酸序列的概率导向分离(pins) - Google Patents

核苷酸序列的概率导向分离(pins) Download PDF

Info

Publication number
CN105189781B
CN105189781B CN201380073618.4A CN201380073618A CN105189781B CN 105189781 B CN105189781 B CN 105189781B CN 201380073618 A CN201380073618 A CN 201380073618A CN 105189781 B CN105189781 B CN 105189781B
Authority
CN
China
Prior art keywords
target dna
sample
dna molecule
pcr
pins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201380073618.4A
Other languages
English (en)
Chinese (zh)
Other versions
CN105189781A (zh
Inventor
托马斯·奎斯特
玛丽·尤斯特·米克尔森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evonik Operations GmbH
Original Assignee
Samplix SARL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Samplix SARL filed Critical Samplix SARL
Publication of CN105189781A publication Critical patent/CN105189781A/zh
Application granted granted Critical
Publication of CN105189781B publication Critical patent/CN105189781B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201380073618.4A 2012-12-21 2013-12-20 核苷酸序列的概率导向分离(pins) Active CN105189781B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA201270822 2012-12-21
DKPA201270822 2012-12-21
PCT/EP2013/077844 WO2014096421A1 (en) 2012-12-21 2013-12-20 Probability-directed isolation of nucleotide sequences (pins)

Publications (2)

Publication Number Publication Date
CN105189781A CN105189781A (zh) 2015-12-23
CN105189781B true CN105189781B (zh) 2017-06-06

Family

ID=49841687

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380073618.4A Active CN105189781B (zh) 2012-12-21 2013-12-20 核苷酸序列的概率导向分离(pins)

Country Status (9)

Country Link
US (2) US10214759B2 (enExample)
EP (1) EP2935617B1 (enExample)
JP (1) JP6522511B2 (enExample)
CN (1) CN105189781B (enExample)
AU (1) AU2013366478A1 (enExample)
CA (1) CA2895904C (enExample)
DK (1) DK2935617T3 (enExample)
ES (1) ES2630377T3 (enExample)
WO (1) WO2014096421A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6522511B2 (ja) * 2012-12-21 2019-05-29 サンプリクス アンパーツゼルスカブ ヌクレオチド配列の確率指向性単離（ｐｉｎｓ）
EP3770271B1 (en) 2015-06-26 2023-02-08 Samplix ApS Targeted enrichment of long nucleotide sequences using microfluidic partitioning
CN105420375B (zh) * 2015-12-24 2020-01-21 北京大学 一种环境微生物基因组草图的构建方法
CN109402223A (zh) * 2018-11-12 2019-03-01 河南弘腾农业有限公司 一种基于动物粪便样品构建测序文库的方法及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004058987A2 (en) * 2002-12-20 2004-07-15 Qiagen Gmbh Nucleic acid amplification
WO2008096923A1 (en) * 2007-02-05 2008-08-14 Korea Research Institute Of Bioscience And Biotechnology Methods for fabrication of uncultivated genome-probing microarrays by amplifying genome derived from a single microbial cell using multiple displacement amplification

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030228586A1 (en) 1999-06-30 2003-12-11 Rafick-Pierre Sekaly Method for measuring de novo T-cell production in humans
JP6522511B2 (ja) * 2012-12-21 2019-05-29 サンプリクス アンパーツゼルスカブ ヌクレオチド配列の確率指向性単離（ｐｉｎｓ）

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004058987A2 (en) * 2002-12-20 2004-07-15 Qiagen Gmbh Nucleic acid amplification
WO2008096923A1 (en) * 2007-02-05 2008-08-14 Korea Research Institute Of Bioscience And Biotechnology Methods for fabrication of uncultivated genome-probing microarrays by amplifying genome derived from a single microbial cell using multiple displacement amplification

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Amp-PCR:Combining a Random Unbiased Phi29-Amplification with a Specific Real-Time PCR,Performed in One Tube to Increase PCR Sensitivity;Lena E et al.;《Plos one》;20101231;第5卷(第12期);第1-7页 *
Molecule by molecule PCR amplification of complex DNA mixtures for direct sequencing: an approach to in vitro cloning;Lukyanov KA et al.;《Nucleic acids research》;19960601;第24卷(第11期);第2194-2195页 *
Routes to improving the reliability of low level DNA analysis using real-time PCR;Stephen LR et al.;《BMC biotechnology》;20060706;第6卷(第1期);第1-11页 *

Also Published As

Publication number Publication date
EP2935617B1 (en) 2017-04-12
CN105189781A (zh) 2015-12-23
US10214759B2 (en) 2019-02-26
US20190233865A1 (en) 2019-08-01
AU2013366478A1 (en) 2015-07-09
WO2014096421A1 (en) 2014-06-26
JP2016500276A (ja) 2016-01-12
JP6522511B2 (ja) 2019-05-29
US11078507B2 (en) 2021-08-03
DK2935617T3 (en) 2017-07-17
EP2935617A1 (en) 2015-10-28
ES2630377T3 (es) 2017-08-21
CA2895904C (en) 2021-06-08
CA2895904A1 (en) 2014-06-26
US20160053291A1 (en) 2016-02-25

Similar Documents

Publication Publication Date Title
JP6262877B2 (ja) 環状ｄｎａの増幅方法
CN106460066A (zh) 通过液滴分选的核苷酸序列排除富集(needls)
US11078507B2 (en) Probability-directed isolation of nucleotide sequences (PINS)
Huang et al. Suppression Subtractive Hybridization (SSH) and its modifications in microbiological research
WO2023098492A1 (zh) 测序文库构建方法及应用
CN112280878B (zh) 一种检测副溶血性弧菌的特异性靶点、引物、检测方法及应用
JP2016500276A5 (enExample)
CN105992827A (zh) 用于测序测定的通用对照
CN107937581A (zh) 用于乳酸菌测序的扩增引物对、乳酸菌种属鉴定方法及应用
JP2015528281A (ja) 拡大された基質範囲を有する新規のｄｎａポリメラーゼ
CN112813200B (zh) 核酸极短pcr扩增的方法、检测方法及应用
JP5470400B2 (ja) 生物学的試料中の低量ｒｎａ種の特異的検出方法
Liu et al. Epigenetic segregation of microbial genomes from complex samples using restriction endonucleases HpaII and McrB
CN117947195B (zh) 用于检测沙门氏菌的一步法CRISPR/Cas12b检测试剂盒及方法
CN116200475B (zh) 一种检测tet(X)基因及其同源基因的简并引物对及其方法
Yamura et al. Non-amplification nucleic acid detection with thio-NAD cycling
CN111793623B (zh) 62个多等位snp-ngs的分型遗传标记组合物、试剂盒、鉴定体系以及分型方法
WO2023283495A1 (en) Crispr-based protein barcoding and surface assembly
van Zyl et al. Engineering resistance to phage GVE3 in Geobacillus thermoglucosidasius
JP6112652B2 (ja) 標的ｄｎａの特異的増幅方法
JP2025510976A (ja) マイコバクテリアを検出するためのプライマー及びプローブ
KR20250000989A (ko) 바실러스 리케니포르미스 k12 균주 동정용 프라이머 쌍 및 이의 용도
McBee et al. Multiplex transcriptional characterizations across diverse and hybrid bacterial cell-free expression systems
JP5189747B2 (ja) 黄色ブドウ球菌のコアグラーゼ型別用オリゴヌクレオチド
CN119307628A (zh) 幽门螺杆菌左氧氟沙星耐药基因快速可视化检测系统及方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20180903

Address after: Denmark Herlev

Patentee after: Degussa

Address before: Luxemburg Luxemburg

Patentee before: SAMPLIX S.A.R.L

TR01 Transfer of patent right