CN105164259B - 核酸的分离 - Google Patents
核酸的分离 Download PDFInfo
- Publication number
- CN105164259B CN105164259B CN201480023809.4A CN201480023809A CN105164259B CN 105164259 B CN105164259 B CN 105164259B CN 201480023809 A CN201480023809 A CN 201480023809A CN 105164259 B CN105164259 B CN 105164259B
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- sample
- composition
- lysate
- samples
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13156609 | 2013-02-25 | ||
| EP13156609.3 | 2013-02-25 | ||
| PCT/EP2014/053154 WO2014128129A1 (en) | 2013-02-25 | 2014-02-18 | Isolation of nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105164259A CN105164259A (zh) | 2015-12-16 |
| CN105164259B true CN105164259B (zh) | 2018-02-27 |
Family
ID=47749705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201480023809.4A Active CN105164259B (zh) | 2013-02-25 | 2014-02-18 | 核酸的分离 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10196673B2 (cg-RX-API-DMAC10.html) |
| EP (2) | EP2958997B1 (cg-RX-API-DMAC10.html) |
| JP (1) | JP6567427B2 (cg-RX-API-DMAC10.html) |
| CN (1) | CN105164259B (cg-RX-API-DMAC10.html) |
| CA (1) | CA2901641C (cg-RX-API-DMAC10.html) |
| ES (1) | ES2741956T3 (cg-RX-API-DMAC10.html) |
| WO (1) | WO2014128129A1 (cg-RX-API-DMAC10.html) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| EP3901280B1 (en) | 2012-10-17 | 2025-03-12 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| CN111500680B (zh) | 2013-06-25 | 2024-04-16 | 普罗格诺西斯生物科学公司 | 检测样品中生物靶标的空间分布的方法和系统 |
| EP4321627A3 (en) | 2015-04-10 | 2024-04-17 | 10x Genomics Sweden AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| EP3782731A1 (en) * | 2015-06-10 | 2021-02-24 | Biocartis N.V. | Improved detection of methylated dna |
| CN105861659A (zh) * | 2016-04-13 | 2016-08-17 | 武汉康录生物技术有限公司 | 一种用于荧光原位杂交ffpe组织切片的预处理液及预处理方法 |
| EP3586129B1 (en) | 2017-03-28 | 2023-07-12 | Phase Scientific International, Ltd. | Method for accurate diagnosis of a disease targeting biomarkers in liquid biopsy |
| SG11201909324WA (en) | 2017-06-01 | 2019-11-28 | Yin To Chiu | Phase separation behavior modifying agents for aqueous two-phase separation within porous material |
| WO2019046563A1 (en) | 2017-09-01 | 2019-03-07 | Phase Diagnostics, Inc. | METHOD AND DEVICE FOR USING AQUEOUS BIPHASE SYSTEMS (ATPS) TO IMPROVE THE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS |
| US20200318153A1 (en) * | 2017-09-01 | 2020-10-08 | Phase Diagnostics, Inc. | Method and device of using aqueous two-phase systems (ATPS) for enhancing diagnostics for dental and oral diseases |
| US20200208137A1 (en) | 2017-09-18 | 2020-07-02 | Yin To Chiu | Method for Using Aqueous Two-Phase System for the Isolation, Purification and/or Concentration of Short Nucleic Acid Fragments |
| EP3740588A4 (en) | 2018-01-19 | 2021-10-20 | Phase Scientific International, Ltd. | METHOD OF ISOLATION AND PURIFICATION OF NUCLEIC ACIDS USING A SOLID-LIQUID PHASE SYSTEM |
| WO2019143895A1 (en) | 2018-01-19 | 2019-07-25 | Yin To Chiu | Spontaneous nucleic acid purification and concentration in a single step |
| CN111868256A (zh) | 2018-01-23 | 2020-10-30 | 比奥卡尔齐斯股份有限公司 | 用于分析解离解链曲线数据的方法 |
| US12157124B2 (en) | 2019-11-06 | 2024-12-03 | 10X Genomics, Inc. | Imaging system hardware |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| EP4752521A3 (en) | 2019-12-23 | 2026-08-12 | 10X Genomics, Inc. | Reversible fixing reagents and methods of use thereof |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| BR112022018784A2 (pt) | 2020-03-20 | 2023-01-31 | Phase Scient International Ltd | Composições e métodos para extração de ácido ribonucleico |
| US12416603B2 (en) | 2020-05-19 | 2025-09-16 | 10X Genomics, Inc. | Electrophoresis cassettes and instrumentation |
| EP4153775B1 (en) | 2020-05-22 | 2024-07-24 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4164796A4 (en) | 2020-06-10 | 2024-03-06 | 10x Genomics, Inc. | Fluid delivery methods |
| CN116194590A (zh) | 2020-09-18 | 2023-05-30 | 10X基因组学有限公司 | 样品处理设备和图像配准方法 |
| KR20230098259A (ko) | 2020-10-29 | 2023-07-03 | 비오까르띠 엔브이 | 멀티플렉스 핵산 검출을 위한 제네릭 카트리지 및 방법 |
| ES2989523T3 (es) | 2021-02-19 | 2024-11-26 | 10X Genomics Inc | Método de uso de un dispositivo modular de soporte para ensayos |
| US12553805B2 (en) | 2021-08-02 | 2026-02-17 | 10X Genomics, Inc. | Methods of preserving a biological sample |
| USD1064308S1 (en) | 2021-09-17 | 2025-02-25 | 10X Genomics, Inc. | Sample handling device |
| EP4441711A1 (en) | 2021-12-20 | 2024-10-09 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
| NL2034698B1 (en) | 2023-04-26 | 2024-11-04 | Biocartis Nv | Semi-solid phase extraction of nucleic acids using a quaternary salt and cation mixture in alkaline conditions |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1922317A (zh) * | 2003-12-24 | 2007-02-28 | 3M创新有限公司 | 使用微流体装置和浓缩步骤的核酸分离用方法和试剂盒 |
| CN1922331A (zh) * | 2003-10-16 | 2007-02-28 | 第三次浪潮技术公司 | 体液的直接核酸检测 |
| CN102146112A (zh) * | 2011-01-25 | 2011-08-10 | 天根生化科技（北京）有限公司 | 从福尔马林固定石蜡包埋组织中提取脱氧核糖核酸的方法 |
| WO2012075133A1 (en) * | 2010-11-30 | 2012-06-07 | Life Technologies Corporation | Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples |
| WO2013020089A2 (en) * | 2011-08-04 | 2013-02-07 | Sage Science, Inc. | Systems and methods for processing fluids |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5538870A (en) | 1994-09-21 | 1996-07-23 | Boehringer Mannheim Corporation | Method for preparing nucleic acids for analysis and kits useful therefor |
| JP4503712B2 (ja) | 1996-09-09 | 2010-07-14 | 株式会社島津製作所 | 核酸合成法 |
| US6469159B1 (en) | 1999-04-26 | 2002-10-22 | Robert T. Belly | Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| US20030134292A1 (en) | 2001-10-30 | 2003-07-17 | Farchaus Joseph W. | Thermostable DNA polymerases and methods of making same |
| JP5086250B2 (ja) | 2005-06-23 | 2012-11-28 | ビオカルティ ソシエテ アノニム | 自動医療診断用のカートリッジ、システム及び方法 |
| CN101213022B (zh) | 2005-06-30 | 2010-06-09 | 拜欧卡蒂斯股份公司 | 自动医学诊断盒体 |
| CA2680801A1 (en) | 2007-03-14 | 2008-09-18 | Sierra Molecular Corporation | Compositions, systems, and methods for preservation and/or stabilizationof a cell and/or macromolecule |
| DE102008026058A1 (de) * | 2008-05-30 | 2009-12-03 | Qiagen Gmbh | Lyse, Binde- und/oder Waschreagenz verwendbar zur Isolierung und/oder Reinigung von Nukleinsäuren |
| WO2010118540A1 (en) | 2009-04-14 | 2010-10-21 | Biocartis Sa | Treatment of a sample with focused acoustic energy |
| EP2454378B1 (en) | 2009-07-17 | 2015-12-23 | Canon U.S. Life Sciences, Inc. | Methods and systems for dna isolation on a microfluidic device |
| WO2011094577A2 (en) * | 2010-01-29 | 2011-08-04 | Micronics, Inc. | Sample-to-answer microfluidic cartridge |
| GB2483858A (en) | 2010-09-21 | 2012-03-28 | Univ Hull | Amplifying nucleic acids using microfluidic device to perform PRC |
| WO2013002354A1 (ja) | 2011-06-29 | 2013-01-03 | 株式会社ダナフォーム | 生体試料の前処理方法、ｒｎａの検出方法及び前処理キット |
-
2014
- 2014-02-18 WO PCT/EP2014/053154 patent/WO2014128129A1/en not_active Ceased
- 2014-02-18 US US14/768,871 patent/US10196673B2/en active Active
- 2014-02-18 EP EP14705753.3A patent/EP2958997B1/en active Active
- 2014-02-18 EP EP19179423.9A patent/EP3674406A1/en not_active Withdrawn
- 2014-02-18 CA CA2901641A patent/CA2901641C/en active Active
- 2014-02-18 CN CN201480023809.4A patent/CN105164259B/zh active Active
- 2014-02-18 JP JP2015558426A patent/JP6567427B2/ja active Active
- 2014-02-18 ES ES14705753T patent/ES2741956T3/es active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1922331A (zh) * | 2003-10-16 | 2007-02-28 | 第三次浪潮技术公司 | 体液的直接核酸检测 |
| CN1922317A (zh) * | 2003-12-24 | 2007-02-28 | 3M创新有限公司 | 使用微流体装置和浓缩步骤的核酸分离用方法和试剂盒 |
| WO2012075133A1 (en) * | 2010-11-30 | 2012-06-07 | Life Technologies Corporation | Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples |
| CN102146112A (zh) * | 2011-01-25 | 2011-08-10 | 天根生化科技（北京）有限公司 | 从福尔马林固定石蜡包埋组织中提取脱氧核糖核酸的方法 |
| WO2013020089A2 (en) * | 2011-08-04 | 2013-02-07 | Sage Science, Inc. | Systems and methods for processing fluids |
Non-Patent Citations (3)
| Title |
|---|
| A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability;Christopher J. Easley等;《PNAS》;20061219;第103卷(第51期);第19276页左栏第4段至第19277页右栏第2段，第19275页右栏第二段 * |
| A Rapid and Simple Procedure for the Routine Detection of Ras Point Mutations in Formalin-fixed, Paraffin-embedded Tissues;RJ Slebos等;《Diagnostic Molecular Pathology the American Journal of Surgical Pathology Part B》;19920601;第1卷(第2期);第136-141页 * |
| Final Report on the Safety Assessment of Oleth-2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -15, -16, -20, -23, -25, -30, -40, -44, and -501;W Bergfeld等;《International Journal of Toxicology》;19990901;第18卷(第1期);第17-24页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US20160002706A1 (en) | 2016-01-07 |
| EP2958997B1 (en) | 2019-07-17 |
| CA2901641C (en) | 2021-02-09 |
| CN105164259A (zh) | 2015-12-16 |
| WO2014128129A1 (en) | 2014-08-28 |
| EP3674406A1 (en) | 2020-07-01 |
| JP2016507242A (ja) | 2016-03-10 |
| CA2901641A1 (en) | 2014-08-28 |
| US10196673B2 (en) | 2019-02-05 |
| JP6567427B2 (ja) | 2019-08-28 |
| EP2958997A1 (en) | 2015-12-30 |
| ES2741956T3 (es) | 2020-02-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6567427B2 (ja) | 核酸の単離 | |
| Pös et al. | Technical and methodological aspects of cell-free nucleic acids analyzes | |
| Enderle et al. | Characterization of RNA from exosomes and other extracellular vesicles isolated by a novel spin column-based method | |
| Erickson et al. | Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples | |
| JP6562389B2 (ja) | 核酸の精製方法 | |
| JP2017140053A (ja) | Ｒｎａおよびｄｎａの並行単離および／または並行精製のためのプロセス | |
| US20080003574A1 (en) | Kits for RNA extraction | |
| JP2018186836A (ja) | 固定生物学的試料から生体分子を抽出するためのターゲット細胞または組織を決定するための方法 | |
| Bridge | Reverse transcription–polymerase chain reaction molecular testing of cytology specimens: Pre‐analytic and analytic factors | |
| CN104755628A (zh) | 生物样品的稳定化 | |
| JP2014500513A (ja) | ワックスで包埋された生物学的試料を処理するための方法およびキット | |
| Roeder et al. | Body fluid identification using mRNA profiling | |
| JP2018520706A (ja) | 試料採取からｎｇｓライブラリ調製までの自動化 | |
| US20180087048A1 (en) | Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples | |
| CN100429307C (zh) | 核酸检测方法及其系统 | |
| Zhou et al. | Rapid removal of cytology slide coverslips for DNA and RNA isolation | |
| WO2025003501A1 (en) | Nucleic acid preparation | |
| Fuessel et al. | Quantification of microRNAs in urine-derived specimens | |
| Khokhar et al. | Evaluation of Maxwell® 16 for automated DNA extraction from whole blood and formalin-fixed paraffin embedded (FFPE) tissue. | |
| Jakubowska et al. | mRNA profiling for vaginal fluid and menstrual blood identification | |
| EP3559271A1 (en) | Fully automated nucleic acid extraction method for tissue samples | |
| EP4701784A1 (en) | Semi-solid phase extraction of nucleic acids using a quaternary salt and cation mixture in alkaline conditions | |
| Bauer et al. | Optimized high quality DNA extraction from formalin-fixed paraffin-embedded human atherosclerotic lesions | |
| Field et al. | Laser microdissection for gene expression profiling | |
| Nechifor-Boilă et al. | Evaluation of a DNA extraction and purification protocol using archived formalin-fixed paraffin-embedded tissues for BRAF mutations analysis in papillary thyroid Microcarcinomas |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20151216 Assignee: Guangzhou Wanfu catis Biotechnology Co., Ltd Assignor: Wanfu Cadiz Co., Ltd Contract record no.: X2020990000658 Denomination of invention: Isolation of nucleic acid Granted publication date: 20180227 License type: Common License Record date: 20201203 |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| CP01 | Change in the name or title of a patent holder |
Address after: Mechelen, Belgium Patentee after: Baiocatis biology Co.,Ltd. Address before: Mechelen, Belgium Patentee before: BIOCARTIS N.V. |
|
| CP01 | Change in the name or title of a patent holder |