CN105154359A - Biological agent for decreasing content of heavy metal cadmium in rice and production method thereof - Google Patents
Biological agent for decreasing content of heavy metal cadmium in rice and production method thereof Download PDFInfo
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Abstract
The invention discloses a biological agent for decreasing content of heavy metal cadmium in rice and a production method thereof. The biological agent comprises a bacterium WH14, a bacterium Wh16, a bacterium WH19, a bacterium WH20, plant ash, activated organic material and vermiculite. The production method of the biological agent includes the steps of mixing proportionally the bacteria WH14, WH16, WH19 and WH20 to obtain a WH colony, subjecting the WH colony to secondary bacterial culture via a WH colony secondary medium, and mixing the plant ash, the vermiculite, the activated organic material and chaff bacteria of the WH colony according to a formula, fermenting an obtained mixture at normal temperature for over 10 days, and performing crushing and packaging to obtain the biological agent. By using the biological agent to decrease the content of toxic heavy metal cadmium in soil, rice absorbs less heavy metal cadmium, and the effect is significant.
Description
Technical field
The present invention relates to grain heavy metal degradation technique field, particularly the biotechnological formulation of heavy metal cadmium content and manufacture method thereof in a kind of rice of degrading.
Background technology
In last century Mo to the beginning of this century, be, after we Aisa people enters industrialization, caused harm the most serious age by heavy metal.First be that Japanese paddy rice suffers cadmium pollution and has the sequela such as itai-itai.Taiwan is no exception.So when this century alternately, the research of heavy metal contamination comes into one's own especially.The cadmium content of such as rice varieties Jian Indica rice strain is higher than Japonica rice cadmium content.Among heavy metal, nickel and plumbous comparatively other elements more easily spread, and invade from water inlet and water port.The security quality standard Taiwan of international rice heavy metal will drop to 0.2mg/g by existing 0.45mg/g.Farmland, Taiwan renovates with different eluent process that to be treated to engineering method with sour drip washing in the soil method by heavy metal contamination simple, and treatment effect is good, and required time is short, and economic benefit is better, and the characteristic such as adjustable treatment capacity and being accepted.Especially to the soil of cadmium pollution, clearance can reach more than 80%, has standard containing heavy metal to ploughing at Taiwan soil and irrigation water.
China has become one of the most serious country of environmental problem.According to statistics by last century Mo, the contaminated cultivated area of China reaches more than 2,000 ten thousand hectares, accounts for 1/5th of cultivated area.Every year because soil pollution grain drop in production just reaches 1,000 ten thousand tons, also have 1,200 ten thousand ton-grain foods contaminated.In the material of contaminated soil, heavy metal contamination accounts for critical role.On March 28th, 2011, environmental protection special campaigns in this year " are pointed sword at " heavy metal pollution problem by the joint meeting of national environmental protection Bu Deng nine ministries and commissions.Investigation display, You Jin40% farmland, urban, Pearl River Delta heavy metal pollution of vegetable field soil exceeds standard, and wherein 10% belongs to severe overweight.Generally can remain decades at heavy metal in soil, wherein lead can remain 100 years.
The repairing and treating of heavy metal contamination agricultural land soil have multiple by way of.One is engineering control, such as, dig, soil removal and replacement, but implements complicated, costly and easily reduces soil fertility.Two is chemical harnessings, and effect and expense are all moderate, and shortcoming is easy reactivation.Three is that agricultural is administered, and easy to operately expends lower, but the cycle is long, and effect is not remarkable.
If the toxicity of heavy metal in soil cadmium can be reduced, the intake of rice heavy metal cadmium just significantly can be reduced.
Summary of the invention
The object of the present invention is to provide biotechnological formulation and the manufacture method thereof of heavy metal cadmium content in a kind of rice of degrading, utilize this biotechnological formulation to reduce heavy metal cadmium poisonous in soil; WH flora contained by this biotechnological formulation is the microorganism that can reduce heavy metal cadmium in rice, the life carrier that it is microorganism WH flora with plant ash, acitve organic matter material, vermiculite, the metabolic activity of this microorganism can cause solvency action, settlement action, sequestering action, biological methylation, cell inner accumulation or volatilization, thus is removed by heavy metal in soil cadmium.
Technical solution proposed by the invention is such: a kind of microorganism manufacturing the biotechnological formulation of heavy metal cadmium content in degraded rice, described microorganism is by bacillus cereus (Bacilluscereus, depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center, be called for short CGMCC, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, preservation date is on December 28th, 2012, deposit number is CGMCCNO.7062, WH14 bacterial classification by name); Aspergillus oryzae (Aspergillusoryzae, depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center, be called for short CGMCC, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, preservation date is on December 28th, 2012, deposit number is CGMCCNO.7064, WH16 by name); Viride (Trichodermaviride, depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center, be called for short CGMCC, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, preservation date is on December 28th, 2012, deposit number is CGMCCNO.7067, WH19 bacterial classification by name); Pseudomonas aeruginosa (Pseudomonasaeruginosa, depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center, be called for short CGMCC, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, preservation date is on January 8th, 2013, deposit number is CGMCCNO.7111, WH20 bacterial classification by name) composition.
In the degraded rice adopting mentioned microorganism to make, the main component of the biotechnological formulation of heavy metal cadmium content is WH14 bacterial classification, WH16 bacterial classification, WH19 bacterial classification, WH20 bacterial classification and plant ash, acitve organic matter material, vermiculite.
This biotechnological formulation includes inoculating proportion:
(1) WH14 bacterial classification: 10-45%; (2) WH16 bacterial classification: 13-50%;
(3) WH19 bacterial classification: 8-40%; (4) WH20 bacterial classification: 8-37%.
In degraded rice of the present invention, the manufacture method of the biotechnological formulation of heavy metal cadmium content, comprises spawn culture and preparation, and its processing step is:
(1) WH14 bacterial classification, WH16 bacterial classification, WH19 bacterial classification, WH20 bacterial classification are mixed by above-mentioned weight percent proportioning consist of WH flora;
(2) described WH flora secondary medium is filled a prescription by weight percentage:
Acitve organic matter material 35-45%; Rice bran 35-45%; Soyflour 15-17%; Milk powder 1.5-2.5%; Sugar 0.5-1.5%; All the other water;
(3) second class inoculum is cultivated: first mixed thoroughly by described formula mixing by WH flora secondary medium, WH flora is inoculated under normal temperature, secondary medium temperature range 30 DEG C-60 DEG C, pH value 6.5-7.5, incubation time 7-15 days, cultivate the ripe rear active chaff bacterium obtaining WH flora, it is containing viable count 5 hundred million-30 hundred million/gram;
(4) by the active chaff bacterium of plant ash, acitve organic matter material, vermiculite and WH flora, by following weight percent formula mixing, normal temperature fermentation more than 10 days, pulverizing, the biotechnological formulation making heavy metal cadmium content in degraded rice is packed:
Plant ash 10-70%;
Acitve organic matter material 15-40%;
Vermiculite 5-10%;
WH bacterial activity chaff bacterium 10-40%.
The present invention is the biotechnological formulation of heavy metal cadmium content in a kind of rice of degrading utilizing biotechnology to be developed into, which utilizes WH flora heavy metal particularly heavy metal cadmium strong absorption effect and realize degrade.This microbial preparation is composited after pure culture by the multiple beneficial microorganism through strictly screening, and can reduce cadmium content in rice after being manured into soil; Obviously can improve soil nutrient supply situation, the various microbial inoculums mainly through WH flora promote dissolving and the release of heavy metal in soil cadmium and other heavy metals.Microbial inoculum discharges a large amount of inorganic organic acidic substances in metabolic process simultaneously, promotes release and the chelating of Trace Elements in Soil silicon, aluminium, iron, magnesium, molybdenum etc., improves the supply situation of soil nutrient.
In degraded rice provided by the present invention, the biotechnological formulation of heavy metal cadmium content is a kind of through Exploration & stu dy for many years, the significant microorganism of performance is tamed out from nature separation, mutagenesis, screening, use modern technique production application in the biotechnological formulation administering heavy metal cadmium in rice, it can reduce the toxicity of heavy metal in soil cadmium, and then reduces the intake of rice heavy metal cadmium significantly.On year-on-year basis in chemical method degraded heavy metal cadmium, this biotechnological formulation is more effective, safer, more economical, more practical, and effectively can also improve the physics of soil, chemistry and biological nature while degraded heavy metal cadmium, thus be conducive to the development of plant growth and agriculture production.
Compared with prior art, the present invention has following significant effect:
(1) this biotechnological formulation, under the effect of WH flora, effectively reduces the toxicity of heavy metal cadmium in soil, rice, can reduce the intake of rice heavy metal cadmium significantly, repair by the soil of heavy metal cadmium, protect environment.
(2) use this biotechnological formulation can improve soil constantly, increase the soil organism, improve soil water-reataining fertilizer-keeping and venting capability, promote plant growth.
(3) using this biotechnological formulation to reducing the heavy metal cadmium content effect of rice clearly, the heavy metal cadmium content of rice can be reduced to the level be harmful to human health, thus be of value to HUMAN HEALTH.
The biological material specimens depositary institution title that the present invention relates to, address, preservation date and numbering, and the Classification And Nomenclature of this biomaterial is as following table:
| Depositary institution | Address | Preservation date | Deposit number | Classification And Nomenclature |
| China Committee for Culture Collection of Microorganisms's common micro-organisms center, is called for short CGMCC | No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica | On December 28th, 2012 | CGMCC NO. 7062 | Bacillus cereus Bacillus cereus |
| China Committee for Culture Collection of Microorganisms's common micro-organisms center, is called for short CGMCC | No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica | On December 28th, 2012 | CGMCC NO. 7064 | Aspergillus oryzae Aspergillus oryzae |
| China Committee for Culture Collection of Microorganisms's common micro-organisms center, is called for short CGMCC | No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica | On December 28th, 2012 | CGMCC NO.7067 | Viride Trichoderma viride |
| China Committee for Culture Collection of Microorganisms's common micro-organisms center, is called for short CGMCC | No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica | On January 8th, 2013 | CGMCC NO.7111 | Pseudomonas aeruginosa Pseudomonas aeruginosa |
Embodiment
By embodiment below, the present invention is further elaborated.
Embodiment 1
In a kind of rice of degrading, the main component of the biotechnological formulation of heavy metal cadmium content is WH14 bacterial classification, WH16 bacterial classification, WH19 bacterial classification, WH20 bacterial classification and plant ash, acitve organic matter material, vermiculite.
In mentioned microorganism, WH14 bacterial classification is bacillus cereus (Bacilluscereus); WH16 bacterial classification is aspergillus oryzae (Aspergillusoryzae); WH19 bacterial classification is viride (Trichodermaviride); WH20 bacterial classification is Pseudomonas aeruginosa (PseudomonasAeruginosa).
In this degraded rice, the biotechnological formulation of heavy metal cadmium content includes the proportioning of bacterial classification for (weight percent):
(1) WH14 bacterial classification: 10%; (2) WH16 bacterial classification: 13%;
(3) WH19 bacterial classification: 40%; (4) WH20 bacterial classification: 37%.
In this degraded rice, the manufacture method of the biotechnological formulation of heavy metal cadmium content comprises spawn culture and preparation, and its processing step is:
(1) above-mentioned WH14 bacterial classification, WH16 bacterial classification, WH19 bacterial classification, WH20 bacterial classification are mixed by the proportioning of above-mentioned weight percent consist of WH flora;
(2) WH flora secondary medium formula (weight percent) is:
Acitve organic matter material 40%, rice bran 40%, soyflour 16%, milk powder 2%, sugar 1%, all the other water;
(3) second class inoculum is cultivated, and is first mixed thoroughly by above-mentioned formula mixing by WH flora secondary medium, inoculates WH flora under normal temperature, substratum temperature 60 C, pH value 7.0, incubation time 7 days, cultivate the ripe rear active chaff bacterium obtaining WH flora, 3,000,000,000/gram can be reached containing viable bacteria;
(4) by the active chaff bacterium of plant ash, acitve organic matter material, vermiculite, WH flora, by following weight percent formula mixing, normal temperature fermentation more than 10 days, pulverizing, the biotechnological formulation making heavy metal cadmium content in the degraded rice with living microorganism is packed:
Plant ash 10%;
Acitve organic matter material 40%;
Vermiculite 10%;
The active chaff bacterium 40% of WH flora.
Embodiment 2
In the degraded rice of the present embodiment, the proportioning of each composition of biotechnological formulation of heavy metal cadmium content and parameter and example 1 difference are:
1, in this degraded rice, the biotechnological formulation of heavy metal cadmium content includes the proportioning of bacterial classification for (weight percent):
(1) WH14 bacterial classification: 26%; (2) WH16 bacterial classification: 50%;
(3) WH19 bacterial classification: 8%; (4) WH20 bacterial classification: 16%.
2, the proportioning (weight percent) of second class inoculum substratum is: acitve organic matter material 35%, rice bran 45%, soyflour 17%, milk powder 1.5%, sugar 0.5%, all the other water.
3, second class inoculum substratum temperature is 45 DEG C, pH value 6.5, and incubation time 10 days, can reach 1,500,000,000/gram containing viable count.
4, by the active chaff bacterium of plant ash, acitve organic matter material, vermiculite and WH flora, by following weight percent formula mixing, normal temperature fermentation more than 10 days, pulverizing, the biotechnological formulation making heavy metal cadmium content in the degraded rice with living microorganism is packed:
Plant ash 38%;
Acitve organic matter material 30%;
Vermiculite 7%;
The active chaff bacterium 25% of WH flora.
The proportioning of all the other each compositions, each parameter and processing step are with example 1.
Embodiment 3
In the degraded rice of the present embodiment, the proportioning of each composition of biotechnological formulation of heavy metal cadmium content and each parameter and example 1 difference are:
1, in this degraded rice, the biotechnological formulation of heavy metal cadmium content includes the proportioning of bacterial classification for (weight percent):
(1) WH14 bacterial classification: 45%; (2) WH16 bacterial classification: 30%;
(3) WH19 bacterial classification: 17%; (4) WH20 bacterial classification: 8%.
2, the proportioning (weight percent) of second class inoculum substratum is: acitve organic matter material 45%, rice bran 35%, soyflour 15%, milk powder 2.5%, sugar 1.5%, all the other water.
3, second class inoculum substratum temperature is 30 DEG C, pH value 7.5, and incubation time 15 days, can reach 500,000,000/gram containing viable count.
4, by the active chaff bacterium of plant ash, acitve organic matter material, vermiculite and WH flora, by following weight percent formula mixing, normal temperature fermentation more than 10 days, pulverizing, the biotechnological formulation making heavy metal cadmium content in the degraded rice with living microorganism is packed:
Plant ash 70%;
Acitve organic matter material 15%;
Vermiculite 5%;
The active chaff bacterium 10% of WH flora.
The proportioning of all the other each compositions, each parameter and processing step are with example 1.
Embodiment 4: one of Comparison study example
The bio-bacterial manure defending party to the application that in microorganism disclosed by above-described embodiment 1,2,3 and degraded rice, the formula range of biotechnological formulation of heavy metal cadmium content is produced is referred to as " gold is without track ".
Test name: use " gold is without track " bio-bacterial manure to the impact of heavy metal cadmium content in rice
1, test period: in March, 2013-2013 year July.
2, test sample: Hunan is Xian No. 45 paddy early.
3, test design: 5 process are established in this test, repeats for 3 times.
Process 1: conventional fertilizer application;
Process 2: conventional fertilizer application+base is executed " gold without track " 50 kgs/acre;
Process 3: conventional fertilizer application+base is executed " gold without track " 75 kgs/acre;
Process 4: conventional fertilizer application+base is executed " gold without track " 100 kgs/acre;
Process 5: conventional fertilizer application+base is executed " gold without track " 125 kgs/acre.
4, each process rice heavy metal cadmium content of mensuration of sampling after paddy rice maturation, result is as following table:
5, interpretation of result:
Can find out from measurement result, after using this " gold is without track " bio-bacterial manure, in rice, heavy metal cadmium content is reduced in various degree, and the range of decrease is 23%-57%, Be very effective.
Embodiment 5: Comparison study example two
Test name: Liling repairing heavy metal in soil pollutes plot experiment
1, test period: in July, 2014-2014 year October.
2, excellent 518 paddy of test sample: H.
3, test design: 4 process are established in this test, repeats for 3 times.
Process 1: conventional fertilizer application;
Process 2: conventional fertilizer application+base liming 100 kgs/acre;
Process 3: commercially available soil conditioner 30 kgs/acre executed by conventional fertilizer application+base;
Process 4: bio-bacterial manure 150 kgs/acre that conventional fertilizer application+base is executed " gold is without track ".
4, each process rice heavy metal cadmium content of mensuration of sampling after paddy rice maturation, result is as following table:
5, interpretation of result:
Can find out from measurement result, after using " gold is without track " bio-bacterial manure, in rice, heavy metal cadmium content reduces by 60.4%, Be very effective.
Embodiment 6: Comparison study example three
Test name: Youxian County's repairing heavy metal in soil pollutes field comparative's test.
1, test period: in July, 2014-2014 year October.
2, test sample: five Feng You 369 paddy.
3, test design: 3 process are established in this test, do not establish repetition.
Process 1: conventional fertilizer application;
Process 2: commercially available soil conditioner 30 kgs/acre executed by conventional fertilizer application+base;
Process 3: bio-bacterial manure 150 kgs/acre that conventional fertilizer application+base is executed " gold is without track 1 ".
4, each process rice heavy metal cadmium content of mensuration of sampling after paddy rice maturation, result is as following table:
5, interpretation of result:
Can find out from measurement result, after using this " gold is without track " bio-bacterial manure, in rice, heavy metal cadmium content reduces by 42.9%, Be very effective.
Claims (4)
1. manufacture a microorganism for the biotechnological formulation of heavy metal cadmium content in degraded rice, it is characterized in that: described microorganism is by bacillus cereus (Bacilluscereus), and deposit number is CGMCCNO.7062, WH14 bacterial classification by name; Aspergillus oryzae (Aspergillusoryzae), deposit number is CGMCCNO.7064, WH16 by name; Viride (Trichodermaviride), deposit number is CGMCCNO.7067, WH19 bacterial classification by name; Pseudomonas aeruginosa (Pseudomonasaeruginosa), deposit number is CGMCCNO.7111, WH20 bacterial classification by name.
2. adopt a biotechnological formulation for heavy metal cadmium content in the degraded rice of microorganism described in claim 1, it is characterized in that: its major ingredient is WH14 bacterial classification, WH16 bacterial classification, WH19 bacterial classification, WH20 bacterial classification and plant ash, acitve organic matter material, vermiculite.
3. the biotechnological formulation of heavy metal cadmium content in degraded rice according to claim 2, is characterized in that: in this degraded rice, the biotechnological formulation of heavy metal cadmium content includes inoculating proportion and is by weight percentage:
(1) WH14 bacterial classification: 10-45%; (2) WH16 bacterial classification: 13-50%;
(3) WH19 bacterial classification: 8-40%; (4) WH20 bacterial classification: 8-37%.
4. to degrade according to Claims 2 or 3 the manufacture method of the biotechnological formulation of heavy metal cadmium content in rice, comprise spawn culture and preparation, it is characterized in that:
(1) WH14 bacterial classification, WH16 bacterial classification, WH19 bacterial classification, WH20 bacterial classification are mixed by weight percent proportioning according to claim 3 consist of WH flora;
(2) described WH flora secondary medium is filled a prescription by weight percentage:
Acitve organic matter material 35-45%; Rice bran 35-45%; Soyflour 15-17%; Milk powder 1.5-2.5%; Sugar 0.5-1.5%; All the other water;
(3) second class inoculum is cultivated: first mixed thoroughly by described formula mixing by WH flora secondary medium, WH flora is inoculated under normal temperature, secondary medium temperature range 30 DEG C-60 DEG C, pH value 6.5-7.5, incubation time 7-15 days, cultivate the ripe rear active chaff bacterium obtaining WH flora, it is containing viable count 5 hundred million-30 hundred million/gram;
(4) by the active chaff bacterium of plant ash, acitve organic matter material, vermiculite and WH flora, by following weight percent formula mixing, normal temperature fermentation more than 10 days, pulverizing, the biotechnological formulation making heavy metal cadmium content in degraded rice is packed:
Plant ash 10-70%;
Acitve organic matter material 15-40%;
Vermiculite 5-10%;
WH bacterial activity chaff bacterium 10-40%.
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| CN108192631A (en) * | 2017-12-26 | 2018-06-22 | 湖南恒凯环保科技投资有限公司 | A kind of charcoal base flora composite soil conditioner and its preparation and application |
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| CN112280710A (en) * | 2020-10-27 | 2021-01-29 | 佛山金葵子植物营养有限公司 | Microbial fertilizer suitable for coastal saline-alkali soil sea rice and preparation method thereof |
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