CN105136753B - 滤光系统、荧光观测系统和执行荧光观测的方法 - Google Patents
滤光系统、荧光观测系统和执行荧光观测的方法 Download PDFInfo
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- CN105136753B CN105136753B CN201510403893.9A CN201510403893A CN105136753B CN 105136753 B CN105136753 B CN 105136753B CN 201510403893 A CN201510403893 A CN 201510403893A CN 105136753 B CN105136753 B CN 105136753B
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- 238000000034 method Methods 0.000 title claims abstract description 12
- 230000005540 biological transmission Effects 0.000 claims abstract description 130
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- 230000003287 optical effect Effects 0.000 claims abstract description 32
- 230000000903 blocking effect Effects 0.000 claims description 72
- 238000001914 filtration Methods 0.000 claims description 31
- 238000002834 transmittance Methods 0.000 claims description 31
- KSFOVUSSGSKXFI-GAQDCDSVSA-N CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O Chemical compound CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O KSFOVUSSGSKXFI-GAQDCDSVSA-N 0.000 claims description 11
- 229950003776 protoporphyrin Drugs 0.000 claims description 11
- 238000002073 fluorescence micrograph Methods 0.000 claims description 10
- 230000001419 dependent effect Effects 0.000 claims description 6
- 238000002594 fluoroscopy Methods 0.000 claims description 5
- 238000003384 imaging method Methods 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 19
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- 238000001917 fluorescence detection Methods 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 238000012935 Averaging Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000000295 emission spectrum Methods 0.000 description 2
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- 238000001514 detection method Methods 0.000 description 1
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- 238000010586 diagram Methods 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B5/00—Optical elements other than lenses
- G02B5/20—Filters
- G02B5/208—Filters for use with infrared or ultraviolet radiation, e.g. for separating visible light from infrared and/or ultraviolet radiation
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B5/00—Optical elements other than lenses
- G02B5/20—Filters
- G02B5/22—Absorbing filters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6471—Special filters, filter wheel
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Toxicology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
- Optical Filters (AREA)
- Endoscopes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102014008243.1A DE102014008243A1 (de) | 2014-05-27 | 2014-05-27 | Fluoreszenbeobachtungssystem und optisches Filtersystem hierfür |
| DE102014008243.1 | 2014-05-27 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105136753A CN105136753A (zh) | 2015-12-09 |
| CN105136753B true CN105136753B (zh) | 2019-04-12 |
Family
ID=53489754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510403893.9A Active CN105136753B (zh) | 2014-05-27 | 2015-05-27 | 滤光系统、荧光观测系统和执行荧光观测的方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9958392B2 (enExample) |
| EP (1) | EP2950129B1 (enExample) |
| JP (1) | JP6716201B2 (enExample) |
| CN (1) | CN105136753B (enExample) |
| DE (1) | DE102014008243A1 (enExample) |
| ES (1) | ES2836899T3 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102014016850B9 (de) | 2014-11-13 | 2017-07-27 | Carl Zeiss Meditec Ag | Optisches System zur Fluoreszenzbeobachtung |
| DE102015216570A1 (de) * | 2015-08-31 | 2016-11-03 | Carl Zeiss Meditec Ag | Mikroskopiesystem |
| DE102015011429B9 (de) * | 2015-09-01 | 2020-01-09 | Carl Zeiss Meditec Ag | Optisches Filtersystem und Fluoreszenzbeobachtungssystem |
| EP3205257B1 (en) * | 2016-02-15 | 2022-11-16 | Leica Instruments (Singapore) Pte Ltd | Illumination filter for an illumination filter system of a surgical multispectral fluorescence microscope having a spatial filter pattern |
| DE102017203448B9 (de) | 2017-03-02 | 2021-12-23 | Carl Zeiss Meditec Ag | Mikroskopiesystem und Mikroskopieverfahren zum Quantifizieren einer Fluoreszenz |
| EP3422072B1 (en) | 2017-06-30 | 2020-01-08 | Leica Instruments (Singapore) Pte. Ltd. | Illumination system, microscope comprising an illumination system and microscope method |
| DE102018111958B4 (de) * | 2018-05-17 | 2025-07-17 | Carl Zeiss Meditec Ag | Filtersatz, System und Verfahren zur Beobachtung von Protoporphyrin IX |
| DE102018114695B9 (de) * | 2018-06-19 | 2020-05-28 | Carl Zeiss Meditec Ag | Filtersatz, System und Verfahren zur gleichzeitigen Anregung und Beobachtung von Protoporphyrin IX und Fluorescein |
| EP3805838B1 (en) | 2019-10-10 | 2023-12-06 | Leica Instruments (Singapore) Pte. Ltd. | Microscope and related apparatuses, methods and computer programs |
| DE102020131374B4 (de) | 2020-11-26 | 2024-02-08 | Dionex Softron Gmbh | Fluoreszenzdetektion |
| DE102022125852A1 (de) * | 2022-10-06 | 2024-04-11 | Karl Storz Se & Co. Kg | Medizinische Bildgebungsvorrichtung und Verfahren zur medizinischen Bildgebung |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5127730A (en) | 1990-08-10 | 1992-07-07 | Regents Of The University Of Minnesota | Multi-color laser scanning confocal imaging system |
| JP3621704B2 (ja) | 1995-09-26 | 2005-02-16 | カール．ストルツ．ゲゼルシャフト．ミット．ベシュレンクテル．ハフツング．ウント．カンパニー | 光力学的診断用装置 |
| DE19548913A1 (de) | 1995-12-31 | 1997-07-03 | Storz Karl Gmbh & Co | Vorrichtung zur photodynamischen Diagnose |
| US20010046673A1 (en) * | 1999-03-16 | 2001-11-29 | Ljl Biosystems, Inc. | Methods and apparatus for detecting nucleic acid polymorphisms |
| US6899675B2 (en) * | 2002-01-15 | 2005-05-31 | Xillix Technologies Corp. | Fluorescence endoscopy video systems with no moving parts in the camera |
| US7580185B2 (en) | 2002-08-28 | 2009-08-25 | Carl Zeiss Surgical Gmbh | Microscopy system, microscopy method and a method of treating an aneurysm |
| JP2006509573A (ja) | 2002-12-13 | 2006-03-23 | イエトメド リミテッド | 手術中に腫瘍と正常組織とを実時間で区別するのに特に有用な光学的検査方法および装置 |
| DE102005005253B4 (de) * | 2004-02-11 | 2018-06-28 | Carl Zeiss Ag | Fluoreszenzbeobachtungssystem und Fluoreszenzbeobachtungsverfahren |
| DE102005008925A1 (de) * | 2005-02-24 | 2006-09-07 | Leica Microsystems Cms Gmbh | Laser-Mikrodissektionsgerät |
| DE102005048006A1 (de) * | 2005-10-06 | 2007-04-12 | Carl Zeiss Surgical Gmbh | Mikroskopiesystem und Aufnahmeverfahren zur Sichtbarmachung einer Fluoreszenz |
| DE102006015272A1 (de) * | 2006-04-01 | 2007-10-04 | Carl Zeiss Microimaging Gmbh | Spektralfilter-Set für LED-basierte Mikroskopbeleuchtungen |
| JP2009122203A (ja) * | 2007-11-12 | 2009-06-04 | Shimadzu Corp | 光学フィルタ、蛍光分析用フィルタセット及びその光学フィルタを用いた蛍光測定装置 |
| DE102008034008B4 (de) * | 2008-07-21 | 2010-07-01 | Carl Zeiss Surgical Gmbh | Filtersatz zur Beobachtung von Fluoreszenzstrahlung in biologischem Gewebe |
| DE102009058663B4 (de) | 2009-12-16 | 2024-07-25 | Karl Storz Se & Co. Kg | Verfahren zum Prüfen einer optischen Vorrichtung |
| DE102010033825B9 (de) * | 2010-08-09 | 2024-04-18 | Carl Zeiss Meditec Ag | Fluoreszenzbeobachtungssystem und Filtersatz |
| DE102010044503B4 (de) * | 2010-09-06 | 2017-11-02 | Leica Microsystems (Schweiz) Ag | Fluoreszenz-Operations-Stereomikroskop |
| DE102011002990B4 (de) * | 2011-01-21 | 2014-02-27 | Carl Zeiss Meditec Ag | Visualisieren von Gewebe in einem Operationsbereich |
| JP2014036759A (ja) * | 2012-08-17 | 2014-02-27 | Hoya Corp | 電子内視鏡システムおよび内視鏡用光源装置 |
| CN103529506A (zh) * | 2013-09-27 | 2014-01-22 | 华侨大学 | 一种紫外荧光滤光片及其制备方法 |
| CN103735249B (zh) * | 2013-12-27 | 2015-04-22 | 中国科学院苏州生物医学工程技术研究所 | 荧光检测仪 |
-
2014
- 2014-05-27 DE DE102014008243.1A patent/DE102014008243A1/de not_active Ceased
-
2015
- 2015-05-26 JP JP2015106256A patent/JP6716201B2/ja active Active
- 2015-05-27 EP EP15001596.4A patent/EP2950129B1/en active Active
- 2015-05-27 CN CN201510403893.9A patent/CN105136753B/zh active Active
- 2015-05-27 US US14/722,265 patent/US9958392B2/en active Active
- 2015-05-27 ES ES15001596T patent/ES2836899T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2950129A1 (en) | 2015-12-02 |
| CN105136753A (zh) | 2015-12-09 |
| EP2950129B1 (en) | 2020-10-28 |
| US20150346098A1 (en) | 2015-12-03 |
| ES2836899T3 (es) | 2021-06-28 |
| JP2015225344A (ja) | 2015-12-14 |
| JP6716201B2 (ja) | 2020-07-01 |
| DE102014008243A1 (de) | 2015-12-03 |
| US9958392B2 (en) | 2018-05-01 |
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