CN105110991A - Water-soluble composite biological bacterial fertilizer and preparation method thereof - Google Patents
Water-soluble composite biological bacterial fertilizer and preparation method thereof Download PDFInfo
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- CN105110991A CN105110991A CN201510616624.0A CN201510616624A CN105110991A CN 105110991 A CN105110991 A CN 105110991A CN 201510616624 A CN201510616624 A CN 201510616624A CN 105110991 A CN105110991 A CN 105110991A
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- 239000002131 composite material Substances 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 239000003337 fertilizer Substances 0.000 title abstract description 18
- 230000001580 bacterial effect Effects 0.000 title abstract 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 41
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 19
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims abstract description 7
- 235000019837 monoammonium phosphate Nutrition 0.000 claims abstract description 7
- 239000006012 monoammonium phosphate Substances 0.000 claims abstract description 7
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims abstract description 7
- 229910052939 potassium sulfate Inorganic materials 0.000 claims abstract description 7
- 235000011151 potassium sulphates Nutrition 0.000 claims abstract description 7
- 210000003608 fece Anatomy 0.000 claims description 32
- 239000010871 livestock manure Substances 0.000 claims description 32
- 150000001875 compounds Chemical class 0.000 claims description 31
- 239000007788 liquid Substances 0.000 claims description 25
- 241000233866 Fungi Species 0.000 claims description 24
- 239000003795 chemical substances by application Substances 0.000 claims description 24
- 244000061458 Solanum melongena Species 0.000 claims description 13
- 235000002597 Solanum melongena Nutrition 0.000 claims description 13
- 229920000742 Cotton Polymers 0.000 claims description 11
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 10
- 238000000855 fermentation Methods 0.000 claims description 10
- 230000004151 fermentation Effects 0.000 claims description 10
- 239000011591 potassium Substances 0.000 claims description 10
- 229910052700 potassium Inorganic materials 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 9
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 3
- 241000726221 Gemma Species 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 abstract description 7
- 238000003973 irrigation Methods 0.000 abstract description 4
- 230000002262 irrigation Effects 0.000 abstract description 4
- 239000003621 irrigation water Substances 0.000 abstract description 4
- 244000063299 Bacillus subtilis Species 0.000 abstract description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract description 3
- 241000881860 Paenibacillus mucilaginosus Species 0.000 abstract description 3
- 239000007633 bacillus mucilaginosus Substances 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 230000000813 microbial effect Effects 0.000 abstract description 3
- 239000002054 inoculum Substances 0.000 abstract 2
- 208000035240 Disease Resistance Diseases 0.000 abstract 1
- 238000005507 spraying Methods 0.000 abstract 1
- 241000219146 Gossypium Species 0.000 description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 8
- 239000002609 medium Substances 0.000 description 8
- 239000002689 soil Substances 0.000 description 5
- 244000068988 Glycine max Species 0.000 description 4
- 235000010469 Glycine max Nutrition 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 4
- 235000019341 magnesium sulphate Nutrition 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- 239000003905 agrochemical Substances 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 238000005056 compaction Methods 0.000 description 2
- 239000013530 defoamer Substances 0.000 description 2
- GNTDGMZSJNCJKK-UHFFFAOYSA-N divanadium pentaoxide Chemical compound O=[V](=O)O[V](=O)=O GNTDGMZSJNCJKK-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000031968 Cadaver Diseases 0.000 description 1
- 241000790917 Dioxys <bee> Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- CHWRSCGUEQEHOH-UHFFFAOYSA-N potassium oxide Chemical compound [O-2].[K+].[K+] CHWRSCGUEQEHOH-UHFFFAOYSA-N 0.000 description 1
- 229910001950 potassium oxide Inorganic materials 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Abstract
The invention discloses a water-soluble composite biological bacterial fertilizer and a preparation method thereof. The water-soluble composite biological bacterial fertilizer is prepared from the following components in part by weight: 40-60 parts of BSFA, 30-50 parts of monoammonium phosphate, 5-15 parts of potassium sulfate and 0.5-1.5 parts of complex microbial inoculants. The complex microbial inoculants consist of bacillus subtilis, bacillus megatherium and bacillus mucilaginosus. The invention also provides the preparation method of the water-soluble composite biological bacterial fertilizer. The water-soluble composite biological bacterial fertilizer is powder, and can be soluble in water completely; and when the water-soluble composite biological bacterial fertilizer is applied, the water-soluble composite biological bacterial fertilizer is mainly used with irrigation water through trickle irrigation and tube spraying. The water-soluble composite biological bacterial fertilizer has the advantages of yield improvement, disease resistance and environmental protection. Requirements of water-saving irrigation can be met.
Description
Technical field
The invention belongs to bio-bacterial manure field, particularly a kind of Water Soluble Compound bio-bacterial manure and preparation method thereof.
Background technology
At present, in agricultural production process, most distinct issues mainly contain the soil compaction used chemical pesticide in a large number and cause, the residual and fertilising using chemical pesticide to cause separates with agricultural chemicals and carry out causing labor waste, and these problems cause serious hindrance to agriculture production and increasing crop yield.
Along with the application of microbial technique in agricultural, for realizing environmental protection, pollution-free and high-efficiency agriculture produces and provides effective means and solution route, and many function yeast are also widely used in agricultural chemicals and fertilizer field.But current bio-feritlizer as complementary fertilizer, can only make the consumption of chemical fertilizer and agricultural chemicals reduce, but can not fundamentally solve the problem such as soil compaction and environmental pollution.
Summary of the invention
For above-mentioned demand, the present invention provides a kind of Water Soluble Compound bio-bacterial manure and preparation method thereof especially.
Object of the present invention can be achieved through the following technical solutions:
A kind of Water Soluble Compound bio-bacterial manure, be made up of the component comprising following weight part:
Wherein, described composite fungus agent is made up of subtilis (Bacillussubtilis), bacillus megaterium (Bacillusmegaterium) and bacillusmusilaginosiengineering (Bacillusmucilaginosus).
Component and the weight part content of described Water Soluble Compound bio-bacterial manure are preferably as follows:
Wherein, described composite fungus agent is made up of subtilis, bacillus megaterium and bacillusmusilaginosiengineering.
Subtilis described in described composite fungus agent is 5-10 hundred million cfu/g, described bacillus megaterium is 5-10 hundred million cfu/g, described bacillusmusilaginosiengineering is 2-10 hundred million cfu/g.
Subtilis described in described composite fungus agent is 7.5 hundred million cfu/g, described bacillus megaterium is 7.5 hundred million cfu/g, described bacillusmusilaginosiengineering is 500,000,000 cfu/g.
A preparation method for Water Soluble Compound bio-bacterial manure, comprises the following steps:
Take potassium fulvate 40-60 weight part, monoammonium phosphate 30-50 weight part, potassium sulfate 5-15 weight part and composite fungus agent 0.5-1.5 weight part, after stirring, obtain Water Soluble Compound bio-bacterial manure.
Wherein, described composite fungus agent is made up of subtilis (Bacillussubtilis), bacillus megaterium (Bacillusmegaterium) and bacillusmusilaginosiengineering (Bacillusmucilaginosus).
The preparation of described composite fungus agent comprises the following steps:
(1) subtilis is placed aseptically, carry out the cultivation of eggplant bottle successively, shaking table is cultivated, fermentor cultivation, obtains fermented liquid A;
(2) bacillus megaterium is placed aseptically, carry out the cultivation of eggplant bottle successively, shaking table is cultivated, fermentor cultivation, obtains fermented liquid B;
(3) bacillusmusilaginosiengineering is placed aseptically, carry out the cultivation of eggplant bottle successively, shaking table is cultivated, fermentor cultivation, obtains fermented liquid C;
(4) to take etc. described fermented liquid A, the described fermented liquid B of weight and described fermented liquid C, mix, concentrate drying, obtain composite fungus agent.
The actual conditions that described eggplant bottle is cultivated is: under aseptic condition in eggplant bottle substratum, cultivates 36 hours at 30 ± 2 DEG C of temperature;
The actual conditions that described shaking table is cultivated is: under aseptic condition in Medium of shaking flask fermentation, under pH7.0,30 DEG C of conditions, 180r/min shaking table cultivates 12 hours;
The actual conditions of described fermentor cultivation is: under aseptic condition in liquid fermentation medium, at pH7.0, tank pressure 0.5kg/cm
2, 30 DEG C, under ventilation 1: 0.8-1.1 condition, cultivate after 48-60 hour, viable count is greater than 80.0 × 10
8cfu/mL, 80% thalline becomes gemma tank at present, obtains fermented liquid.
Described eggplant bottle substratum comprises following component: extractum carnis 3g, peptone 10g, sodium-chlor 5g, agar 15g and water 1000mL;
Described Medium of shaking flask fermentation comprises following component: sucrose 10g, extractum carnis 10g, soybean cake powder 5g, potassium primary phosphate 0.5g, magnesium sulfate 0.5g and water 1000mL;
Described liquid fermentation medium comprises following component: cotton seed powder 21Kg, soybean cake powder 14kg, magnesium sulfate 3.5Kg, ammonium sulfate 3.5kg, sucrose 14kg, peptone 2.1kg and defoamer 50mL, add water to 700L.
Invention further provides the application of above-mentioned Water Soluble Compound bio-bacterial manure in cotton planting.
Invention further provides above-mentioned Water Soluble Compound bio-bacterial manure when dripping irrigation water, pipeline spray water together with use, every mu of consumption 30-50kg.
Compared with prior art, its beneficial effect is in the present invention:
(1) Water Soluble Compound bio-bacterial manure of the present invention organically combines organic matter, biological bacteria and nitrogen, phosphorus, potassium, improve the organic matter of soil, supplement again nitrogen, phosphorus, potassium that crop growth needs, improve soil, improve utilization rate of fertilizer, increase production and improve productivity.
(2) Water Soluble Compound bio-bacterial manure of the present invention is by the rational proportion of multiple-microorganism, has the advantages such as volume increase, disease-resistant, environmental protection.
(3) Water Soluble Compound bio-bacterial manure of the present invention, its preparation method is simple, is easy to suitability for industrialized production.
(4) complete water-soluble composite biological fertilizer of the present invention is pulvis, can be entirely water-soluble, water suction moisturizing, mainly use together with drip irrigation, pipeline sprinkling etc. with irrigation water when using, can soil fertility be increased, reduce artificial, assist corps nutrient to absorb, prevention multiple kinds of crops disease, effectively improves utilization rate of fertilizer, meets the needs of water saving irrigation.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
The preparation of eggplant bottle substratum: take extractum carnis 3g, peptone 10g, sodium-chlor 5g, agar 15g and water 1000mL, stir.
The preparation of Medium of shaking flask fermentation: take sucrose 10g, extractum carnis 10g, soybean cake powder 5g, phosphoric acid dioxy potassium 0.5g, magnesium sulfate 0.5g and water 1000mL, stir.
The preparation of liquid fermentation medium: take cotton seed powder 21Kg, soybean cake powder 14kg, magnesium sulfate 3.5Kg, ammonium sulfate 3.5kg, sucrose 14kg, peptone 2.1kg and defoamer 50mL, add water to 700L, stir.
Embodiment 1
Subtilis, bacillus megaterium, bacillusmusilaginosiengineering are placed aseptically respectively, carries out following operation respectively: cultivate at 30 ± 2 DEG C of temperature after 36 hours in eggplant bottle substratum; Put into Medium of shaking flask fermentation, under pH7.0,30 DEG C of conditions, 180r/min shaking table cultivates 12 hours; Put into liquid fermentation medium again, at pH7.0, tank pressure 0.5kg/cm
2, 30 DEG C, under ventilation 1: 0.8-1.1 condition, cultivate after 48-60 hour, viable count is greater than 80.0 × 10
8cfu/mL, 80% thalline becomes gemma tank at present, obtains fermented liquid A, fermented liquid B and fermented liquid C respectively;
Described fermented liquid A, the described fermented liquid B of weight and the described fermented liquid C such as to take, mix, concentrate drying, obtain composite fungus agent.
Embodiment 2
Take potassium fulvate 40kg, monoammonium phosphate 30kg, potassium sulfate 5kg and composite fungus agent 0.5kg, after stirring, obtain Water Soluble Compound bio-bacterial manure.
Wherein, subtilis described in composite fungus agent is 7.5 hundred million cfu/g, described bacillus megaterium is 7.5 hundred million cfu/g, described bacillusmusilaginosiengineering is 500,000,000 cfu/g.
Embodiment 3
Take potassium fulvate 60kg, monoammonium phosphate 50kg, potassium sulfate 15kg and composite fungus agent 1.5kg, after stirring, obtain Water Soluble Compound bio-bacterial manure.
Wherein, subtilis described in composite fungus agent is 7.5 hundred million cfu/g, described bacillus megaterium be 7.5 hundred million cfu/g, described bacillusmusilaginosiengineering is 500,000,000 cfu/g.
Embodiment 4
Take potassium fulvate 50kg, monoammonium phosphate 40kg, potassium sulfate 10kg and composite fungus agent 1kg, after stirring, obtain Water Soluble Compound bio-bacterial manure.
Wherein, subtilis described in composite fungus agent is 500,000,000 cfu/g, described bacillus megaterium is 1,000,000,000 cfu/g, described bacillusmusilaginosiengineering is 1,000,000,000 cfu/g.
Embodiment 5
Take potassium fulvate 50kg, monoammonium phosphate 40kg, potassium sulfate 10kg and composite fungus agent 1kg, after stirring, obtain Water Soluble Compound bio-bacterial manure.
Wherein, subtilis described in composite fungus agent is 1,000,000,000 cfu/g, described bacillus megaterium is 500,000,000 cfu/g, described bacillusmusilaginosiengineering is 200,000,000 cfu/g.
Common Repone K composite fertilizer (nitrogen, Vanadium Pentoxide in FLAKES, potassium oxide mass content are respectively 15%, 15%, 15%) is adopted, urea (nitrogen content 46wt%) in comparative example.
Test site: Xinjiang province Manas County.
Test period: 2012 and 2013 continuous 2 years, for examination cotton variety be middle cotton No. 45.
Fertilizer in the Water Soluble Compound bio-bacterial manure of embodiment of the present invention 2-5 and comparative example is added dripping irrigation water with the consumption of every mu of 40kg when cotton field planting, evenly sprays, cultivation management is all by normal cropping pattern plantation.
The cotton that the cotton adopting the Water Soluble Compound bio-bacterial manure of embodiment of the present invention 2-5 to irrigate comparatively adopts the fertilizer in comparative example to irrigate, the effect of increasing production of every mu specifically asks for an interview table 1.
Table 1
| Yield-increasing efficiency average (%) | |
| Embodiment 2 | 18.8 |
| Embodiment 3 | 24.6 |
| Embodiment 4 | 22.9 |
| Embodiment 5 | 24.6 |
Use the cotton of Water Soluble Compound bio-bacterial manure of the present invention comparatively to adopt the cotton root system of the fertilizer in comparative example more flourishing, and can effectively prevent and treat leaf disease, improve output.
The invention is not restricted to embodiment here, those skilled in the art, according to announcement of the present invention, do not depart from improvement that scope makes and amendment all should within protection scope of the present invention.
Claims (10)
1. a Water Soluble Compound bio-bacterial manure, is characterized in that, is made up of the component comprising following weight part:
2. Water Soluble Compound bio-bacterial manure according to claim 1, is characterized in that, described composite fungus agent is made up of subtilis, bacillus megaterium and bacillusmusilaginosiengineering.
3. Water Soluble Compound bio-bacterial manure according to claim 2, is characterized in that, component and the weight part content of described Water Soluble Compound bio-bacterial manure are preferably as follows:
4. Water Soluble Compound bio-bacterial manure according to claim 2, it is characterized in that, subtilis described in described composite fungus agent is 5-10 hundred million cfu/g, described bacillus megaterium is 5-10 hundred million cfu/g, described bacillusmusilaginosiengineering is 2-10 hundred million cfu/g.
5. Water Soluble Compound bio-bacterial manure according to claim 4, is characterized in that, subtilis described in described composite fungus agent is 7.5 hundred million cfu/g, described bacillus megaterium is 7.5 hundred million cfu/g, described bacillusmusilaginosiengineering is 500,000,000 cfu/g.
6. a preparation method for Water Soluble Compound bio-bacterial manure, is characterized in that, comprises the following steps:
Take potassium fulvate 40-60 weight part, monoammonium phosphate 30-50 weight part, potassium sulfate 5-15 weight part and composite fungus agent 0.5-1.5 weight part, after stirring, obtain Water Soluble Compound bio-bacterial manure.
7. the preparation method of Water Soluble Compound bio-bacterial manure according to claim 6, is characterized in that, described composite fungus agent is made up of subtilis, bacillus megaterium and bacillusmusilaginosiengineering.
8. the preparation method of Water Soluble Compound bio-bacterial manure according to claim 7, is characterized in that, the preparation of described composite fungus agent comprises the following steps:
(1) subtilis is placed aseptically, carry out the cultivation of eggplant bottle successively, shaking table is cultivated, fermentor cultivation, obtains fermented liquid A;
(2) bacillus megaterium is placed aseptically, carry out the cultivation of eggplant bottle successively, shaking table is cultivated, fermentor cultivation, obtains fermented liquid B;
(3) bacillusmusilaginosiengineering is placed aseptically, carry out the cultivation of eggplant bottle successively, shaking table is cultivated, fermentor cultivation, obtains fermented liquid C;
(4) to take etc. described fermented liquid A, the described fermented liquid B of weight and described fermented liquid C, mix, concentrate drying, obtain composite fungus agent.
9. the preparation method of Water Soluble Compound bio-bacterial manure according to claim 8, is characterized in that,
The actual conditions that described eggplant bottle is cultivated is: under aseptic condition in eggplant bottle substratum, cultivates 36 hours at 30 ± 2 DEG C of temperature;
The actual conditions that described shaking table is cultivated is: under aseptic condition in Medium of shaking flask fermentation, under pH7.0,30 DEG C of conditions, 180r/min shaking table cultivates 12 hours;
The actual conditions of described fermentor cultivation is: under aseptic condition in liquid fermentation medium, at pH7.0, tank pressure 0.5kg/cm
2, 30 DEG C, under ventilation 1: 0.8-1.1 condition, cultivate after 48-60 hour, viable count is greater than 80.0 × 10
8cfu/mL, 80% thalline becomes gemma tank at present, obtains fermented liquid.
10. the application of Water Soluble Compound bio-bacterial manure in cotton planting according to any one of claim 1-5.
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| CN105646104A (en) * | 2015-12-30 | 2016-06-08 | 湖南泰谷生物肥料有限公司 | Fully water-soluble composite biological bacterial fertilizer as well as preparation method and application thereof |
| CN105801221A (en) * | 2016-02-23 | 2016-07-27 | 云南金星化工有限公司 | Fully water-soluble microbial fertilizer promoting crop root growth and preparation method thereof |
| CN106966828A (en) * | 2017-03-28 | 2017-07-21 | 赵和红 | A kind of liquid seed manure |
| CN106977294A (en) * | 2017-04-15 | 2017-07-25 | 新疆吉泰高新科技发展股份有限公司 | A kind of humic acid full water soluble fertilizer and preparation method with bioactivity |
| CN110606781A (en) * | 2019-09-17 | 2019-12-24 | 湖南金叶众望科技股份有限公司 | Full-water-soluble organic-inorganic compound fertilizer and preparation method thereof |
| CN110683891A (en) * | 2019-11-26 | 2020-01-14 | 南阳市朴欣肥业有限公司 | Mineral biological organic fertilizer |
| CN111018612A (en) * | 2019-12-20 | 2020-04-17 | 德州市元和农业科技开发有限责任公司 | Full-water-soluble drip-irrigation microbial inoculant particles and preparation method thereof |
| CN115197010A (en) * | 2022-08-18 | 2022-10-18 | 湖南大三湘茶油股份有限公司 | Efficient soil activator for camellia oleifera and preparation method thereof |
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