CN105110988B - A kind of the special nutritional formula of liquid and configuration method of salt stress romaine lettuce - Google Patents
A kind of the special nutritional formula of liquid and configuration method of salt stress romaine lettuce Download PDFInfo
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- CN105110988B CN105110988B CN201510607941.6A CN201510607941A CN105110988B CN 105110988 B CN105110988 B CN 105110988B CN 201510607941 A CN201510607941 A CN 201510607941A CN 105110988 B CN105110988 B CN 105110988B
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Abstract
The invention discloses a kind of special nutritional formula of liquid of salt stress romaine lettuce, it contains the following substances: 945~970 milligrams of four water-calcium nitrate, 607~650 milligrams of potassium nitrate, 115~130 milligrams of ammonium dihydrogen phosphate, 493~503 milligrams of epsom salt, 37.2~40 milligrams of ethylenediamine tetra-acetic acid, 27.8~30 milligrams of ferrous sulfate, 2.86~3.0 milligrams of boric acid, 2.13~2.8 milligrams of manganese sulfate, 0.22~0.31 milligram of zinc sulfate, 0.08~0.1 milligram of copper sulphate, 0.02~0.04 milligram of ammonium molybdate.In above-mentioned formula, one or both of salt, proline, salicylic acid can also be added.The research and development of this formula can fully meet romaine lettuce normal growth under salt stress.Allogenic material (proline, salicylic acid) addition can increase salt-resistance of the romaine lettuce under salt stress.
Description
Technical field
The invention belongs to agriculture soilless cultivation fields, are related to allogenic material to the proprietary formula of salt stress water planting romaine lettuce.
Background technique
The Na of high concentration+/Cl-Cause salt stress, breaks the intracorporal ion of plant and flow of water balance, cause plant poisoning, lead
It causes plant growth to stop, finally causing death, limit the yield (ZHU JK.Cel, 2001) of crop.Salt stress is to plant
Injury is mainly manifested in two aspects, on the other hand one side Ion toxicity is osmotic stress (TESTER M.DAVENPORT
R,2003).Ion toxicity is mainly K necessary to a large amount of Na+ competitive substitution plant growth+, and K+It is more than 50 kinds in plant
Necessary to enzymatic activity, the synthesis of plant vivo protein is also required to K+Participation.The excessive Na of plant interior accumulation simultaneously+Resistance
Hinder the absorption to other nutrition, cause nutrient imbalance, Ion toxicity can inhibit the activity of desmoenzyme, synthesis of albumen etc., do
Normal physiological metabolism process is disturbed, Physiology and biochemistry disorder is caused；And osmotic stress is mainly shown as the Na of high concentration+Cause normal
The decline of the nutrient solution flow of water, makes root system of plant be difficult to be absorbed into moisture (MUNNS R, TESTER M 2008).Proline is to protein
There is certain protective role, enzyme can be prevented to be dehydrated, also serves as the protective agent (Shang Qingmao, Song's generation are clear etc., 2006) of enzyme, this may be dried meat ammonia
Acid can form hydrophilic colloid in aqueous solution, generate the hydrophobic framework that can be interacted with protein
(Rajendrakumar CS, 1994)., salicylic acid (SA) is a kind of important plant endogenous signaling molecule, is not only adjustable plant
Some growth and development processes of object have in Adversity-stressed Plant and alleviate salt stress to germination, seedling growth inhibition
Physiological action (Ding Xiuying, army etc., 2001 still celebrating cyclopentadienyl, scholars Song wait clearly 2007 Singh B, and Usha is K.2003).Meanwhile bigcatkin willow
Acid can promote romaine lettuce leaf cell to absorb water, and improve romaine lettuce to the resistance of warm and humid environment.
Summary of the invention
Goal of the invention: solving salt stress romaine lettuce can normally give birth under allogenic material (proline, salicylic acid) adjusting
It is long, the response of the yield and romaine lettuce of romaine lettuce to salt stress is improved, final solution can be normal in the local romaine lettuce such as saline and alkaline and beach
Growth.
Technical solution: the present invention provides a kind of special nutritional formula of liquid of salt stress romaine lettuce, every liter of the nutrient solutions
In contain the following substances: 945~970 milligrams of four water-calcium nitrate, 607~650 milligrams of potassium nitrate, the milli of ammonium dihydrogen phosphate 115~130
Gram, 493~503 milligrams of epsom salt, 37.2~40 milligrams of ethylenediamine tetra-acetic acid, 27.8~30 milligrams of ferrous sulfate, boric acid
2.86~3.0 milligrams, 2.13~2.8 milligrams of manganese sulfate, 0.22~0.31 milligram of zinc sulfate, 0.08~0.1 milligram of copper sulphate, molybdenum
0.02~0.04 milligram of sour ammonium.
In above-mentioned formula, one or both of salt, proline, salicylic acid can also be added, such as:
Also containing 5844~6000 milligrams of NaCl in every liter of the nutrient solution.
The also NaCl containing 5844~6000 milligrams, 25~30 milligrams of proline in every liter of the nutrient solution.
The also NaCl containing 5844~6000 milligrams, 27.5~30 milligrams of salicylic acids in every liter of the nutrient solution.
Also containing 27.5~30 milligrams of salicylic acids, 25~30 milligrams of proline in every liter of the nutrient solution.
It finds under study for action, if salt, salicylic acid, proline is added simultaneously in above-mentioned formula, and their proportion is as follows:
Also containing 5844~6000 milligrams of NaCl, 27.5~30 milligrams of salicylic acids, 25~30 milligrams in every liter of the nutrient solution
Proline.Using the formula, romaine lettuce under salt stress state, growing way is best, and mda content is minimum, can be effective
Reduce electrolyte leakage caused by salt damage.
The present invention also provides the configuration methods of above-mentioned special nutritional formula of liquid, carry out as follows:
Weigh A liquid drug: 945 milligrams of four water-calcium nitrate, 607 milligrams of potassium nitrate；Weigh B liquid drug: ammonium dihydrogen phosphate 115
Milligram, 493 milligrams of epsom salt；Weigh C liquid drug: 37.2 milligrams of ethylenediamine tetra-acetic acid, 27.8 milligrams of boric acid of ferrous sulfate
2.86 milligrams, 2.13 milligrams of manganese sulfate, 0.22 milligram of zinc sulfate, 0.08 milligram of copper sulphate, 0.02 milligram of ammonium molybdate, ethylenediamine tetraacetic
37.2 milligrams of acetic acid, 27.8 milligrams of ferrous sulfate be individually placed to be dissolved in 100 milliliters of water, be heated to boiling, when temperature drop
When to 60 degrees Celsius, then the solution for dissolving ferrous sulfate is slowly added in edta solution, then one by one by its other medicine
Product are added in ferrous sulfate and ethylenediamine tetra-acetic acid mixed liquor.
Contain 400 milliliters of water in the potassium nitrate.Contain 300 milliliters of water in the carbonated drink magnesium sulfate.
The utility model has the advantages that existing formula is only suitble to romaine lettuce to grow in normal circumstances, and for existing under salt stress environment
There is formula can not meet.The research and development of this formula can fully meet romaine lettuce normal growth under salt stress.Allogenic material (proline,
Salicylic acid) addition can increase salt-resistance of the romaine lettuce under salt stress.
Specific embodiment:
Embodiment 1 (basic nutrient solution)
Formula: include following nutritional ingredient in 1L nutrient solution: 945 milligrams of four water-calcium nitrate, 607 milligrams of potassium nitrate (include
400 milliliters of water), 115 milligrams of ammonium dihydrogen phosphate, 493 milligrams of epsom salt (300 milliliters of water), 37.2 milli of ethylenediamine tetra-acetic acid
Gram, 27.8 milligrams of ferrous sulfate, 2.86 milligrams of boric acid, 2.13 milligrams of manganese sulfate, 0.22 milligram of zinc sulfate, 0.08 milli of copper sulphate
Gram, 0.02 milligram of ammonium molybdate, 37.2 milligrams of ethylenediamine tetra-acetic acid, 27.8 milligrams of ferrous sulfate.
Configuration method: 1. weigh 945 milligrams of four water-calcium nitrate of A liquid drug, and 607 milligrams of potassium nitrate (includes 400 milliliters of water).
2. weighing 115 milligrams of ammonium dihydrogen phosphate of B liquid drug, 493 milligrams of epsom salt (300 milliliters of water).
3. 37.2 milligrams of ethylenediamine tetra-acetic acid of C liquid drug are weighed, 27.8 milligrams of ferrous sulfate, 2.86 milligrams of boric acid, manganese sulfate
2.13 milligrams, 0.22 milligram of zinc sulfate, 0.08 milligram of copper sulphate, 0.02 milligram of ammonium molybdate, 37.2 milligrams of ethylenediamine tetra-acetic acid, sulphur
Sour ferrous 27.8 milligrams are individually placed to 2 beakers and are dissolved and (respectively have 100 milliliters of water in beaker).Ethylenediamine tetra-acetic acid needs to add
Heat to boiling could dissolve, and be slowly added to ethylenediamine tetraacetic when temperature is down to 60 degrees Celsius, then by the solution for dissolving ferrous sulfate
In acetic acid solution.Other drugs are added in ferrous sulfate and ethylenediamine tetra-acetic acid mixed liquor one by one again.
Embodiment 2 (basic nutrient solution+salt)
Roughly the same with embodiment 1, difference is in its formula also containing 5844 milligrams of NaCl.
Embodiment 3 (basic nutrient solution+salt+proline)
Roughly the same with embodiment 1, difference is in its formula the also NaCl containing 5844 milligrams, 25 milligrams of proline.
Embodiment 4 (basic nutrient solution+salt+salicylic acid)
Roughly the same with embodiment 1, difference is in its formula the also NaCl containing 5844 milligrams, 27.5 milligrams of bigcatkin willows
Acid.
Embodiment 5 (basic nutrient solution+salicylic acid+proline)
Roughly the same with embodiment 1, difference is in its formula the also dried meat ammonia containing 27.5 milligrams of salicylic acids, 25 milligrams
Acid.
Embodiment 6 (basic nutrient solution+salt+salicylic acid+proline)
Roughly the same with embodiment 1, difference is in its formula the also NaCl containing 5844 milligrams, 27.5 milligrams of bigcatkin willows
Acid, 25 milligrams of proline.
Embodiment 7 (comparative example)
For the production of salt stress, traditional method is using sand culture and to carry out blade face with ALA (5-ALA)
It sprays, but ALA sprays that rear blade is long and blade is wide smaller than embodiment 6, and ALA is sprayed to romaine lettuce number of blade Regenerated energy
Power is much smaller than embodiment 6.
Nutrient solution obtained by 1~embodiment of above-described embodiment 6 is settled to 1L, adjusting pH value is 6 or so, puts into water
Train the application of romaine lettuce.
With " U.S.'s fast-growing romaine lettuce " for material, test is in March, 2015 in Jiangsu Polytechnic College of Agriculture and Forestry Bei Jiaoquyuan
It is carried out in skill Experimental Base beam-connected greenhouse.Romaine lettuce grows to 3 spires and is colonized in cultivation bed, slow seedling it is latter week to romaine lettuce at
Reason, water planting romaine lettuce 35 days, experimental data was as follows:
It is above exactly the growing state of 1~embodiment of embodiment 7, wherein the growing way of embodiment 6 is best, and phenotype is better than it
His several formulas, and mda content is minimum, reduces electrolyte leakage caused by salt damage reduce membrane lipid peroxidation product-the third two
The accumulation of aldehyde (MDA), can be of fine quality in other formulas in salt stress low-grades.
Claims (2)
1. a kind of special nutritional formula of liquid of salt stress romaine lettuce, it is characterised in that contain following object in every liter of the nutrient solution
Matter: 945 ~ 970 milligrams of four water-calcium nitrate, 607 ~ 650 milligrams of potassium nitrate, 115 ~ 130 milligrams of ammonium dihydrogen phosphate, epsom salt
493 ~ 503 milligrams, 37.2 ~ 40 milligrams of ethylenediamine tetra-acetic acid, 27.8 ~ 30 milligrams of ferrous sulfate, 2.86 ~ 3.0 milligrams of boric acid, sulphur
2.13 ~ 2.8 milligrams of sour manganese, 0.22 ~ 0.31 milligram of zinc sulfate, 0.08 ~ 0.1 milligram of copper sulphate, 0.02 ~ 0.04 milligram of ammonium molybdate,
NaCl5844 ~ 6000 milligram, 27.5 ~ 30 milligrams of salicylic acid, 25 ~ 30 milligrams of proline.
2. the configuration method of special nutritional formula of liquid as described in claim 1, it is characterised in that carry out as follows:
Weigh A liquid drug: 945 milligrams of four water-calcium nitrate, 607 milligrams of potassium nitrate；
Weigh B liquid drug: 115 milligrams of ammonium dihydrogen phosphate, 493 milligrams of epsom salt；
Weigh C liquid drug: 37.2 milligrams of ethylenediamine tetra-acetic acid, 27.8 milligrams of ferrous sulfate, 2.86 milligrams of boric acid, manganese sulfate
2.13 milligrams, 0.22 milligram of zinc sulfate, 0.08 milligram of copper sulphate, 0.02 milligram of ammonium molybdate, 37.2 milligrams of ethylenediamine tetra-acetic acid, sulphur
Sour ferrous 27.8 milligrams are individually placed to be dissolved in 100 milliliters of water, ethylenediamine tetra-acetic acid are heated to boiling, when temperature is down to
At 60 degrees Celsius, then the solution for dissolving ferrous sulfate is slowly added in edta solution, then one by one by other drugs
It is added in ferrous sulfate and ethylenediamine tetra-acetic acid mixed liquor.
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Families Citing this family (7)
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| CN105638417B (en) * | 2016-02-07 | 2018-12-04 | 浙江省农业科学院 | The seedling screening method of resistance to low calcium Chinese cabbage strain |
| CN105693410A (en) * | 2016-04-10 | 2016-06-22 | 山东省农业科学院玉米研究所 | Nutrient solution for relieving salt stress influences on maize seedlings and application of nutrient solution |
| CN106416658A (en) * | 2016-08-21 | 2017-02-22 | 山东省农业科学院玉米研究所 | Method and nutrient solution for improving salt tolerance of salt-tolerant corn at seedling stages and application of nutrient solution |
| CN108901788A (en) * | 2018-04-20 | 2018-11-30 | 无锡佳培科技有限公司 | A kind of cream romaine lettuce soilless culture method |
| CN109678603B (en) * | 2019-01-24 | 2022-01-04 | 西北农林科技大学 | Nutrient solution capable of improving low-temperature resistance of tomatoes and preparation method and application thereof |
| CN112624836A (en) * | 2021-01-07 | 2021-04-09 | 丽水市农林科学研究院 | Glycine-containing aeroponics lettuce nutrient solution, nutrient supplement and preparation method |
| CN113854298A (en) * | 2021-10-13 | 2021-12-31 | 浙江理工大学 | Method for improving salt stress resistance of salvia miltiorrhiza |
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