CN109678603B - Nutrient solution capable of improving low-temperature resistance of tomatoes and preparation method and application thereof - Google Patents

Nutrient solution capable of improving low-temperature resistance of tomatoes and preparation method and application thereof Download PDF

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CN109678603B
CN109678603B CN201910067832.8A CN201910067832A CN109678603B CN 109678603 B CN109678603 B CN 109678603B CN 201910067832 A CN201910067832 A CN 201910067832A CN 109678603 B CN109678603 B CN 109678603B
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nutrient solution
tomatoes
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stirring
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CN109678603A (en
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胡晓辉
张佼
屈锋
朱玉尧
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Northwest A&F University
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions

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  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
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Abstract

The invention belongs to the technical field of plant nutrient solutions, and particularly discloses a nutrient solution capable of improving the low-temperature resistance of tomatoes and a preparation method and application thereof, wherein the nutrient solution comprises urea, potassium nitrate, nitric acid tetrahydrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, ammonium molybdate tetrahydrate, chelated iron, boric acid, manganese sulfate monohydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, 5-aminolevulinic acid and the like; the nutrient solution is used in the flowering and fruiting periods of tomatoes, can fully meet nutrients required in the growth process of the tomatoes, and can obviously improve the low-temperature resistance of the tomatoes, improve the content of effective nutrient substances of the tomatoes and improve the quality of the tomatoes by adjusting the formula and the dosage of the nutrient solution according to different nutrient demand and demand types of the tomatoes in different growth periods.

Description

Nutrient solution capable of improving low-temperature resistance of tomatoes and preparation method and application thereof
Technical Field
The invention belongs to the technical field of plant nutrient solutions, and particularly relates to a nutrient solution capable of improving low-temperature resistance of tomatoes, and a preparation method and application thereof.
Background
The tomato belongs to the genus of tomato of the family Solanaceae, is a temperature-loving vegetable crop, has rich nutrition of tomato fruits, contains rich vitamins and carotene, has excellent health care effect, and is popular with consumers. In recent years, the method is widely applied to off-season facility cultivation due to consumer and market demands. In facility cultivation, due to the fact that soil is often in a state of no rainwater leaching, high temperature, high humidity and high evaporation and crops in facilities are often continuously cultivated, the facility soil has a plurality of problems of secondary salinization, nutrient imbalance, hardening acidification, microorganism system imbalance, continuous cropping obstacle and the like. The soilless culture technology gets rid of the restriction of soil and becomes the most effective means for solving the soil problem in facility production. The substrate cultivation is a main mode of soilless cultivation, and has good physicochemical properties and can provide a proper growing environment for crop roots. The nutrient solution formula is a key factor for success of soilless culture, at present, China still uses international nutrient solution formulas such as Hoagland, garden type and Kawasaki developed decades ago in a large area, but with diversification of soilless culture plant types and culture forms, the formulas can not completely meet the requirements of the existing soilless culture production, and improvement and optimization of the nutrient solution formula are urgently needed aiming at different varieties, growth stages and culture modes. Therefore, the determination of the nutrient content in the substrate and the optimal application amount of the nutrient solution is of great significance to agricultural production.
The low-temperature adverse environment is an inevitable restriction factor faced by autumn and winter tomato in the growth process, and the improvement of the cold resistance of tomato plants has important significance on the high yield and high quality of autumn and winter tomato.
Disclosure of Invention
The nutrient solution is used in the flowering and fruiting periods of tomatoes, can fully meet nutrients required in the growth process of the tomatoes, can obviously improve the low-temperature resistance of the tomatoes by adjusting the formula and the dosage of the nutrient solution according to different nutrient demands and different types of demands of the tomatoes in different growth periods, and simultaneously improves the content of effective nutrient substances of the tomatoes and the quality of the tomatoes.
The specific technical scheme of the invention is as follows:
a nutrient solution capable of improving the low-temperature resistance of tomatoes is characterized in that: comprises the following components: urea, potassium nitrate, nitric acid tetrahydrate, monopotassium phosphate, magnesium sulfate heptahydrate, ammonium molybdate tetrahydrate, chelated iron, boric acid, manganese sulfate monohydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, and 5-aminolevulinic acid;
the components are weighed according to the following parts by mass:
1000-2500 parts of urea, 4000-7300 parts of potassium nitrate, 10000-18000 parts of nitric acid tetrahydrate, 500-900 parts of monopotassium phosphate, 3500-6000 parts of magnesium sulfate heptahydrate, 0.1-0.3 part of ammonium molybdate tetrahydrate, 180-330 parts of chelated iron, 18-35 parts of boric acid, 11-20 parts of manganese sulfate monohydrate, 1.4-2.6 parts of zinc sulfate heptahydrate, 0.5-1.1 parts of copper sulfate pentahydrate and 70-130 parts of 5-aminolevulinic acid.
Preferably, the components are weighed according to the following parts by mass: 1700 parts of urea, 5600 part of potassium nitrate, 13600 part of nitric acid tetrahydrate, 700 parts of monopotassium phosphate, 4800 parts of magnesium sulfate heptahydrate, 0.2 part of ammonium molybdate tetrahydrate, 250 parts of chelated iron, 27 parts of boric acid, 16 parts of manganese sulfate monohydrate, 2.0 parts of zinc sulfate heptahydrate, 0.8 part of copper sulfate pentahydrate and 100 parts of 5-aminolevulinic acid.
Preferably, the nutrient solution further comprises trace elements.
Preferably, the nutrient solution contains macroelement N, P, K, Ca.
The preparation method of the nutrient solution comprises the following steps:
(1) weighing urea, potassium nitrate and calcium nitrate tetrahydrate according to the mass parts, respectively mixing and stirring the urea, the potassium nitrate and the calcium nitrate tetrahydrate with water according to the proportion of 0.17g/L, 0.56g/L and 1.36g/L until the urea, the potassium nitrate and the calcium nitrate are completely dissolved, mixing the obtained aqueous solution, and uniformly stirring the aqueous solution to obtain a solution A;
(2) weighing potassium dihydrogen phosphate and magnesium sulfate heptahydrate according to the mass parts, respectively mixing and stirring the potassium dihydrogen phosphate and the magnesium sulfate heptahydrate with water according to the proportion of 0.07g/L and 0.48g/L until the potassium dihydrogen phosphate and the magnesium sulfate heptahydrate are completely dissolved, and uniformly mixing and stirring the obtained aqueous solution to obtain a solution B;
(3) weighing ammonium molybdate tetrahydrate, chelated iron, boric acid, manganese sulfate monohydrate, zinc sulfate heptahydrate and copper sulfate pentahydrate according to the mass parts, mixing the ammonium molybdate tetrahydrate, the chelated iron, the boric acid, the manganese sulfate monohydrate, the zinc sulfate heptahydrate and the copper sulfate pentahydrate with water according to the proportion of 0.2mg/L, 250mg/L, 27mg/L, 16mg/L, 2.0mg/L and 0.8mg/L respectively, completely stirring the mixture until the mixture is dissolved, and uniformly mixing and stirring the obtained water solution to obtain a solution C;
(4) mixing the solution A, the solution B and the solution C according to the ratio of 2.5:2.5:1, and adding trace elements to prepare a nutrient solution I;
(5) weighing 5-aminolevulinic acid according to the mass parts, mixing and stirring uniformly with water according to the proportion of 0.01g/L, adding the obtained aqueous solution into the nutrient solution I, and stirring uniformly to obtain a nutrient solution II.
The nutrient solution is applied to the flowering and fruiting periods of tomatoes.
The application of the invention comprises the following steps:
(1) irrigating 0.5L of each plant with the nutrient solution I every day in the flowering period and fruiting initial period of the tomatoes; watering once every 3 days by using the nutrient solution II according to the dosage of 0.5L per plant every day;
(2) irrigating by using the nutrient solution I according to the daily volume of 1L per plant in the stage of the tomato bearing fruit prosperity; irrigating once by using the nutrient solution II every 3 days according to the dosage of 1L per plant every day;
(3) irrigating 0.7L of each plant per day by using the nutrient solution I at the later fruiting stage of the tomatoes; and irrigating once every 3 days by using the nutrient solution II according to the dosage of 0.7L per plant every day.
The mass concentration of the macroelement N, P, K, Ca in the nutrient solution is 287mg.L in sequence-1、15.5mg.L-1、312mg.L-1、230mg.L-1
The trace elements of the invention are: fe. B, Mn, Zn, Cu, Mo;
the mass concentrations of the trace elements in the nutrient solution are respectively as follows: fe 3.5 mg/L, B0.5.5 mg/L, Mn 0.52.52 mg/L, Zn 0.05.05 mg/L, Cu 0.02.02 mg/L, Mo 0.01.01 mg/L.
In the practical operation, in order to facilitate transportation, storage and the like to improve the working efficiency, the solution A and the solution B can be concentrated into 200 times of concentrated solution to obtain the solution A and the solution B, the solution C can be concentrated into 500 times of concentrated solution to obtain the solution C, and the solution C is diluted by adding water when in use to reach the concentration of the original solution and then is applied.
The invention has the beneficial effects that:
the invention discloses a nutrient solution capable of improving the low-temperature resistance of tomatoes and a preparation method and application thereof, wherein the nutrient solution is used for the flowering and fruiting periods of the tomatoes, the dosage and the components of the nutrient solution are adjusted in real time according to different nutrient requirements and different types of nutrient element requirements of the tomatoes in different growth stages, the nutrients required in the whole growth process of the tomatoes can be fully met, the low-temperature resistance of the tomatoes is obviously improved, and the survival rate and the growth rate of the tomatoes in low-temperature and extreme environments are improved; in addition, the nutrient solution is used for culturing tomatoes, can also obviously improve the yield of the tomatoes, increase the content of soluble protein, vitamin C, lycopene, soluble polysaccharide and other nutrient substances contained in the tomatoes and improve the quality of the tomatoes.
Detailed Description
The technical solution of the present invention is further illustrated below with reference to specific examples.
Example 1
A nutrient solution capable of improving the low-temperature resistance of tomatoes comprises the following components: urea, potassium nitrate, nitric acid tetrahydrate, monopotassium phosphate, magnesium sulfate heptahydrate, ammonium molybdate tetrahydrate, chelated iron, boric acid, manganese sulfate monohydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, and 5-aminolevulinic acid;
the components are weighed according to the following parts by mass:
1000 parts of urea, 4000 parts of potassium nitrate, 10000 parts of nitric acid tetrahydrate, 500 parts of monopotassium phosphate, 3500 parts of magnesium sulfate heptahydrate, 0.1 part of ammonium molybdate tetrahydrate, 180 parts of chelated iron, 18 parts of boric acid, 11 parts of manganese sulfate monohydrate, 1.4 parts of zinc sulfate heptahydrate, 0.5 part of copper sulfate pentahydrate and 70 parts of 5-aminolevulinic acid;
the nutrient solution contains macroelement N, P, K, Ca with the mass concentration of 287mg.L in sequence-1、15.5mg.L-1、312mg.L-1、230mg.L-1
The preparation method of the nutrient solution comprises the following steps:
(1) weighing urea, potassium nitrate and calcium nitrate tetrahydrate according to the mass parts, respectively mixing and stirring the urea, the potassium nitrate and the calcium nitrate tetrahydrate with water according to the proportion of 0.17g/L, 0.56g/L and 1.36g/L until the urea, the potassium nitrate and the calcium nitrate are completely dissolved, mixing the obtained aqueous solution, and uniformly stirring the aqueous solution to obtain a solution A;
(2) weighing potassium dihydrogen phosphate and magnesium sulfate heptahydrate according to the mass parts, respectively mixing and stirring the potassium dihydrogen phosphate and the magnesium sulfate heptahydrate with water according to the proportion of 0.07g/L and 0.48g/L until the potassium dihydrogen phosphate and the magnesium sulfate heptahydrate are completely dissolved, and uniformly mixing and stirring the obtained aqueous solution to obtain a solution B;
(3) weighing ammonium molybdate tetrahydrate, chelated iron, boric acid, manganese sulfate monohydrate, zinc sulfate heptahydrate and copper sulfate pentahydrate according to the mass parts, mixing the ammonium molybdate tetrahydrate, the chelated iron, the boric acid, the manganese sulfate monohydrate, the zinc sulfate heptahydrate and the copper sulfate pentahydrate with water according to the proportion of 0.2mg/L, 250mg/L, 27mg/L, 16mg/L, 2.0mg/L and 0.8mg/L respectively, completely stirring the mixture until the mixture is dissolved, and uniformly mixing and stirring the obtained water solution to obtain a solution C;
(4) mixing the solution A, the solution B and the solution C according to the proportion of 2.5:2.5:1, and adding trace elements to ensure that the mass concentration of the trace elements respectively reaches: fe 3.5 mg/L, B0.5.5 mg/L, Mn 0.52.52 mg/L, Zn 0.05.05 mg/L, Cu 0.02.02 mg/L, Mo 0.01.01 mg/L to prepare a nutrient solution I;
(5) weighing 5-aminolevulinic acid according to the mass parts, mixing and stirring uniformly with water according to the proportion of 0.01g/L, adding the obtained aqueous solution into the nutrient solution I, and stirring uniformly to obtain a nutrient solution II;
the application of the nutrient solution comprises the following steps:
(1) irrigating 0.5L of each plant with the nutrient solution I every day in the flowering period and fruiting initial period of the tomatoes; watering once every 3 days by using the nutrient solution II according to the dosage of 0.5L per plant every day;
(2) irrigating by using the nutrient solution I according to the daily volume of 1L per plant in the stage of the tomato bearing fruit prosperity; irrigating once by using the nutrient solution II every 3 days according to the dosage of 1L per plant every day;
(3) irrigating 0.7L of each plant per day by using the nutrient solution I at the later fruiting stage of the tomatoes; and irrigating once every 3 days by using the nutrient solution II according to the dosage of 0.7L per plant every day.
Example 2
A nutrient solution capable of improving the low-temperature resistance of tomatoes comprises the following components: urea, potassium nitrate, nitric acid tetrahydrate, monopotassium phosphate, magnesium sulfate heptahydrate, ammonium molybdate tetrahydrate, chelated iron, boric acid, manganese sulfate monohydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, and 5-aminolevulinic acid;
the components are weighed according to the following parts by mass:
2500 parts of urea, 7300 parts of potassium nitrate, 18000 parts of nitric acid tetrahydrate, 900 parts of potassium dihydrogen phosphate, 6000 parts of magnesium sulfate heptahydrate, 0.3 part of ammonium molybdate tetrahydrate, 330 parts of chelated iron, 35 parts of boric acid, 20 parts of manganese sulfate monohydrate, 2.6 parts of zinc sulfate heptahydrate, 1.1 parts of copper sulfate pentahydrate and 130 parts of 5-aminolevulinic acid;
the nutrient solution contains macroelement N, P, K, Ca with the mass concentration of 287mg.L in sequence-1、15.5mg.L-1、312mg.L-1、230mg.L-1
The preparation method of the nutrient solution comprises the following steps:
(1) weighing urea, potassium nitrate and calcium nitrate tetrahydrate according to the mass parts, respectively mixing and stirring the urea, the potassium nitrate and the calcium nitrate tetrahydrate with water according to the proportion of 0.17g/L, 0.56g/L and 1.36g/L until the urea, the potassium nitrate and the calcium nitrate are completely dissolved, mixing the obtained aqueous solution, and uniformly stirring the aqueous solution to obtain a solution A;
(2) weighing potassium dihydrogen phosphate and magnesium sulfate heptahydrate according to the mass parts, respectively mixing and stirring the potassium dihydrogen phosphate and the magnesium sulfate heptahydrate with water according to the proportion of 0.07g/L and 0.48g/L until the potassium dihydrogen phosphate and the magnesium sulfate heptahydrate are completely dissolved, and uniformly mixing and stirring the obtained aqueous solution to obtain a solution B;
(3) weighing ammonium molybdate tetrahydrate, chelated iron, boric acid, manganese sulfate monohydrate, zinc sulfate heptahydrate and copper sulfate pentahydrate according to the mass parts, mixing the ammonium molybdate tetrahydrate, the chelated iron, the boric acid, the manganese sulfate monohydrate, the zinc sulfate heptahydrate and the copper sulfate pentahydrate with water according to the proportion of 0.2mg/L, 250mg/L, 27mg/L, 16mg/L, 2.0mg/L and 0.8mg/L respectively, completely stirring the mixture until the mixture is dissolved, and uniformly mixing and stirring the obtained water solution to obtain a solution C;
(4) mixing the solution A, the solution B and the solution C according to the proportion of 2.5:2.5:1, and adding the trace elements to ensure that the mass concentration of the trace elements in the nutrient solution reaches: fe 3.5 mg/L, B0.5.5 mg/L, Mn 0.52.52 mg/L, Zn 0.05.05 mg/L, Cu 0.02.02 mg/L, Mo 0.01.01 mg/L to prepare a nutrient solution I;
(5) weighing 5-aminolevulinic acid according to the mass parts, mixing and stirring uniformly with water according to the proportion of 0.01g/L, adding the obtained aqueous solution into the nutrient solution I, and stirring uniformly to obtain a nutrient solution II;
the application of the nutrient solution comprises the following steps:
(1) irrigating 0.5L of each plant with the nutrient solution I every day in the flowering period and fruiting initial period of the tomatoes; watering once every 3 days by using the nutrient solution II according to the dosage of 0.5L per plant every day;
(2) irrigating by using the nutrient solution I according to the daily volume of 1L per plant in the stage of the tomato bearing fruit prosperity; irrigating once by using the nutrient solution II every 3 days according to the dosage of 1L per plant every day;
(3) irrigating 0.7L of each plant per day by using the nutrient solution I at the later fruiting stage of the tomatoes; and irrigating once every 3 days by using the nutrient solution II according to the dosage of 0.7L per plant every day.
Example 3
A nutrient solution capable of improving the low-temperature resistance of tomatoes comprises the following components: urea, potassium nitrate, nitric acid tetrahydrate, monopotassium phosphate, magnesium sulfate heptahydrate, ammonium molybdate tetrahydrate, chelated iron, boric acid, manganese sulfate monohydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, and 5-aminolevulinic acid;
the components are weighed according to the following parts by mass: 1700 parts of urea, 5600 part of potassium nitrate, 13600 part of nitric acid tetrahydrate, 700 parts of monopotassium phosphate, 4800 parts of magnesium sulfate heptahydrate, 0.2 part of ammonium molybdate tetrahydrate, 250 parts of chelated iron, 27 parts of boric acid, 16 parts of manganese sulfate monohydrate, 2.0 parts of zinc sulfate heptahydrate, 0.8 part of copper sulfate pentahydrate and 100 parts of 5-aminolevulinic acid;
the nutrient solution contains macroelement N, P, K, Ca with the mass concentration of 287mg.L in sequence-1、15.5mg.L-1、312mg.L-1、230mg.L-1
The preparation method of the nutrient solution comprises the following steps:
(1) weighing urea, potassium nitrate and calcium nitrate tetrahydrate according to the mass parts, respectively mixing and stirring the urea, the potassium nitrate and the calcium nitrate tetrahydrate with water according to the proportion of 0.17g/L, 0.56g/L and 1.36g/L until the urea, the potassium nitrate and the calcium nitrate are completely dissolved, mixing the obtained aqueous solution, and uniformly stirring the aqueous solution to obtain a solution A;
(2) weighing potassium dihydrogen phosphate and magnesium sulfate heptahydrate according to the mass parts, respectively mixing and stirring the potassium dihydrogen phosphate and the magnesium sulfate heptahydrate with water according to the proportion of 0.07g/L and 0.48g/L until the potassium dihydrogen phosphate and the magnesium sulfate heptahydrate are completely dissolved, and uniformly mixing and stirring the obtained aqueous solution to obtain a solution B;
(3) weighing ammonium molybdate tetrahydrate, chelated iron, boric acid, manganese sulfate monohydrate, zinc sulfate heptahydrate and copper sulfate pentahydrate according to the mass parts, mixing the ammonium molybdate tetrahydrate, the chelated iron, the boric acid, the manganese sulfate monohydrate, the zinc sulfate heptahydrate and the copper sulfate pentahydrate with water according to the proportion of 0.2mg/L, 250mg/L, 27mg/L, 16mg/L, 2.0mg/L and 0.8mg/L respectively, completely stirring the mixture until the mixture is dissolved, and uniformly mixing and stirring the obtained water solution to obtain a solution C;
(4) mixing the solution A, the solution B and the solution C according to the proportion of 2.5:2.5:1, and adding the trace elements to ensure that the mass concentration of the trace elements in the nutrient solution reaches: fe 3.5 mg/L, B0.5.5 mg/L, Mn 0.52.52 mg/L, Zn 0.05.05 mg/L, Cu 0.02.02 mg/L, Mo 0.01.01 mg/L to prepare a nutrient solution I;
(5) weighing 5-aminolevulinic acid according to the mass parts, mixing and stirring uniformly with water according to the proportion of 0.01g/L, adding the obtained aqueous solution into the nutrient solution I, and stirring uniformly to obtain a nutrient solution II;
the application of the invention comprises the following steps:
(1) irrigating 0.5L of each plant with the nutrient solution I every day in the flowering period and fruiting initial period of the tomatoes; watering once every 3 days by using the nutrient solution II according to the dosage of 0.5L per plant every day;
(2) irrigating by using the nutrient solution I according to the daily volume of 1L per plant in the stage of the tomato bearing fruit prosperity; irrigating once by using the nutrient solution II every 3 days according to the dosage of 1L per plant every day;
(3) irrigating 0.7L of each plant per day by using the nutrient solution I at the later fruiting stage of the tomatoes; and irrigating once every 3 days by using the nutrient solution II according to the dosage of 0.7L per plant every day.
Comparative experiment
Comparative experiment 1
The tomato experimental variety is 'Babaoli' tomatoes, the experimental time is 8 months in 2018, the experiment lasts for 4 months, and all experimental groups are carried out in the same greenhouse during the experimental period so as to ensure that all experimental groups are in the same environment;
the experimental method comprises the following steps:
experimental group 1: tomatoes were irrigated according to the application procedure described in example 1 using the nutrient solution prepared in example 1;
experimental group 2: the nutrient solution does not contain a 5-aminolevulinic acid component, the other components and the preparation method thereof are the same as in example 1, and the tomatoes are irrigated according to the application steps described in the example;
control group 1: watering tomatoes according to the application procedure described in example 1 using a kazaki tomato nutrient solution;
control group 2: 5-aminolevulinic acid solution with the same concentration as the example is added into nutrient solution of the Kawasaki tomato, and the tomato is irrigated according to the application steps described in the example 1;
the experimental steps are as follows:
(1) adopting a substrate plug seedling, selecting seedlings with consistent growth vigor when the seedlings are four-leaf and one-heart, and planting the seedlings in cultivation bags, wherein the specification of the cultivation bags is 100 multiplied by 20 multiplied by 16cm, and planting three seedlings in each cultivation bag;
(2) dividing tomato seedlings into A, B, C, D groups when the seedlings are about to enter into a flowering period, continuing cultivation, and after entering into the flowering period and a fruiting period, beginning to irrigate nutrient solution, wherein:
group A was irrigated according to the method of experimental group 1;
group B irrigates according to the method of the experimental group 2;
group C was irrigated as described for control group 1;
group D irrigates according to the method of the control group 2;
during the experiment, the tomato yield per mu of different experimental groups is recorded, the content of soluble protein, vitamin C and soluble total sugar in the tomatoes of different experimental groups is measured, and the experiment results are shown in the table 1:
table 1 results of different experimental groups
Figure DEST_PATH_IMAGE001
Note: data are expressed as "mean ± sem". After the data in the same column, different lower case letters show that the difference is obvious (P < 0.05), and tables 2 and 3 are the same;
as can be seen from Table 1, the experimentsCompared with the control group 1, the tomato yield per mu of the group 1 is improved by 16.40 percent, and compared with the experiment group 1 and the control group 2, the tomato yield per mu is improved by 12.79 percent; compared with the control group 1, the tomato yield per mu of the experimental group 2 is improved by 10.44 percent, so that the nutrient solution disclosed by the invention can obviously improve the tomato yield per mu, is not only related to 5-aminolevulinic acid contained in the nutrient solution, but also related to other components, and all the contained components can be mutually cooperated to achieve a better effect, so that the tomato yield per mu is improved by 16.40 percent to achieve 16314kg 667m-2The yield of (a);
in addition, according to the data in the table, the content of soluble protein, vitamin C and soluble total sugar in the tomatoes cultivated in the experimental group 1 is respectively increased by 120%, 4.45% and 32.17% compared with the control group 1; therefore, the nutrient solution is used in the flowering period and the fruiting period of the tomatoes, can improve the content of effective nutrient components of the tomatoes and improve the quality of the tomatoes;
during the experiment period, the content of the malonaldehyde value (MDA) of the tomato is measured in the result full period, and the tomato leaves are taken as a measuring object; the results are shown in table 2:
TABLE 2 determination of the malonaldehyde value (MDA) content (nmol. g) for different experimental groups-1 FW)
Figure 949437DEST_PATH_IMAGE002
As can be seen from Table 2, the MDA content of the experimental group 1 is reduced by 34.02% compared with that of the control group 1, and the MDA content of the experimental group 2 is reduced by 34.02% and 29.54% compared with that of the control group 1; the content of MDA can be used for measuring the peroxidation degree of membrane lipid, when the content of MDA is increased, cell membrane collapse can be caused, the content of MDA in tomato leaves can be obviously reduced, namely, the peroxidation damage of low-temperature stress on the membrane can be effectively relieved, the cold resistance of the tomato is improved, the cold resistance is related to 5-aminolevulinic acid components contained in the nutrient solution and other components, and all the components are compounded with each other, so that the nutrient solution has a better technical effect of reducing the content of MDA;
comparative experiment 2
Selecting the 'Babaoli' tomatoes as experimental varieties, wherein the experimental time begins in 8 months in 2018, the experiment lasts for 4 months, and all experimental groups are carried out in the same greenhouse during the experimental period so as to ensure that all experimental groups are in the same environment;
the test method comprises the following steps:
experimental group 1: tomatoes were irrigated according to the application procedure described in example 1 using the nutrient solution prepared in example 1;
control group 1: calcium carbonate, diammonium phosphate, manganese sulfate and zinc sulfate respectively replace calcium nitrate tetrahydrate, ammonium molybdate tetrahydrate, manganese sulfate monohydrate and zinc sulfate heptahydrate in equal amount in the embodiment 1, the other components and the preparation method thereof are the same as the embodiment 1, and tomatoes are irrigated according to the application steps in the embodiment 1;
the experimental steps are as follows:
(1) adopting a substrate plug seedling, selecting the seedlings with consistent growth vigor when the four leaves and the center of the seedling are planted in the cultivation bag. The specification of the cultivation bag is 100 multiplied by 20 multiplied by 16cm, and three seedlings are fixedly planted in each cultivation bag;
(2) dividing the seedlings in the step (1) into A, B two groups, irrigating the tomatoes in the group A by using the method in the experimental group 1 and irrigating the tomatoes in the group B by using the method in the control group 1 during the flowering and fruiting periods of the tomatoes;
during the experiment, the tomato yield per mu of the experiment group is recorded, and the content of soluble protein, vitamin C and soluble total sugar contained in the tomato is determined by self, and the experiment result is shown in the table 3:
TABLE 3 results of different experimental groups
Group of Yield (kg 667 m)-2) Soluble protein (mg. g)-1) Vitamin C (mg 100 g)-1) Soluble total sugar%
Experimental group 1 16314±361.19a 0.14±0.004a 38.87±0.12a 11.11±0.29a
Control group 1 13529± 211.43b 0.07±0.001b 38.29±0.36b 8.52±0.12b
As can be seen from Table 3, compared with the control group 1, the acre yield of the experimental group is improved by 20.59%, and the soluble protein, vitamin C and soluble total sugar content of the tomatoes are obviously improved, which shows that the nutrient solution has obvious effects on improving the yield and quality of the tomatoes and improving the resistance of the tomatoes.

Claims (3)

1. A nutrient solution capable of improving the low-temperature resistance of tomatoes is characterized in that: comprises the following components: 1000 parts of urea, 4000 parts of potassium nitrate, 10000 parts of calcium nitrate tetrahydrate, 500 parts of monopotassium phosphate, 3500 parts of magnesium sulfate heptahydrate, 0.1 part of ammonium molybdate tetrahydrate, 180 parts of chelated iron, 18 parts of boric acid, 11 parts of manganese sulfate monohydrate, 1.4 parts of zinc sulfate heptahydrate, 0.5 part of copper sulfate pentahydrate and 70 parts of 5-aminolevulinic acid;
the preparation method of the nutrient solution comprises the following steps:
(1) weighing urea, potassium nitrate and calcium nitrate tetrahydrate according to the mass parts, respectively mixing and stirring the urea, the potassium nitrate and the calcium nitrate tetrahydrate with water according to the proportion of 0.17g/L, 0.56g/L and 1.36g/L until the urea, the potassium nitrate and the calcium nitrate are completely dissolved, mixing the obtained aqueous solution, and uniformly stirring the aqueous solution to obtain a solution A;
(2) weighing potassium dihydrogen phosphate and magnesium sulfate heptahydrate according to the mass parts, respectively mixing and stirring the potassium dihydrogen phosphate and the magnesium sulfate heptahydrate with water according to the proportion of 0.07g/L and 0.48g/L until the potassium dihydrogen phosphate and the magnesium sulfate heptahydrate are completely dissolved, and uniformly mixing and stirring the obtained aqueous solution to obtain a solution B;
(3) weighing ammonium molybdate tetrahydrate, chelated iron, boric acid, manganese sulfate monohydrate, zinc sulfate heptahydrate and copper sulfate pentahydrate according to the mass parts, mixing the ammonium molybdate tetrahydrate, the chelated iron, the boric acid, the manganese sulfate monohydrate, the zinc sulfate heptahydrate and the copper sulfate pentahydrate with water according to the proportion of 0.2mg/L, 250mg/L, 27mg/L, 16mg/L, 2.0mg/L and 0.8mg/L respectively, completely stirring the mixture until the mixture is dissolved, and uniformly mixing and stirring the obtained water solution to obtain a solution C;
(4) mixing the solution A, the solution B and the solution C according to the ratio of 2.5:2.5:1 to prepare a nutrient solution I;
(5) weighing 5-aminolevulinic acid according to the mass parts, mixing and stirring uniformly with water according to the proportion of 0.01g/L, adding the obtained aqueous solution into the nutrient solution I, and stirring uniformly to obtain a nutrient solution II;
the nutrient solution is applied to the flowering and fruiting periods of the tomatoes;
the application of the nutrient solution comprises the following steps:
(1) irrigating 0.5L of each plant with the nutrient solution I every day in the flowering period and fruiting initial period of the tomatoes; watering once every 3 days by using the nutrient solution II according to the dosage of 0.5L per plant every day;
(2) irrigating by using the nutrient solution I according to the daily volume of 1L per plant in the stage of the tomato bearing fruit prosperity; irrigating once by using the nutrient solution II every 3 days according to the dosage of 1L per plant every day;
(3) irrigating 0.7L of each plant per day by using the nutrient solution I at the later fruiting stage of the tomatoes; and irrigating once every 3 days by using the nutrient solution II according to the dosage of 0.7L per plant every day.
2. The nutrient solution for improving the low temperature resistance of tomatoes according to claim 1, wherein the nutrient solution comprises: the nutrient solution also comprises trace elements.
3. The nutrient solution for improving the low temperature resistance of tomatoes according to claim 1, wherein the nutrient solution comprises: the nutrient solution contains a macroelement N, P, K, Ca.
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