CN105076727B - A kind of additive improving piglet oxidation resistance - Google Patents
A kind of additive improving piglet oxidation resistance Download PDFInfo
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- CN105076727B CN105076727B CN201510564651.8A CN201510564651A CN105076727B CN 105076727 B CN105076727 B CN 105076727B CN 201510564651 A CN201510564651 A CN 201510564651A CN 105076727 B CN105076727 B CN 105076727B
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Abstract
The invention discloses a kind of additives improving piglet oxidation resistance.The active ingredient of the additive includes vitamin C, folic acid, vitamin A, vitamin D3, vitamin E, vitamin B2, astragalus polyose, sodium bicarbonate, maltodextrin and glucose.Concrete content is：Vitamin C 8~12%, folic acid 0.1~0.3%, vitamin A 0.4~0.9%, vitamin D32.5~5.0%, vitamin E 4~8%, vitamin B20.4~0.7%, astragalus polyose 5~9%, sodium bicarbonate 6~12%, maltodextrin 5~12%, surplus is glucose.According to the amount ratio 1g of additive and livestock drinking water:4L is added in drinking-water or the amount ratio 1g of additive and feed:1kg, which is added in diet, can improve piglet oxidation resistance, improve the piglet general level of the health, and then improve the growth performance of piglet.
Description
Technical field
The invention belongs to feed additive fields, are related to a kind of additive improving piglet oxidation resistance.
Background technology
Nutrient source turns to solid feed from breast milk after weaned piglet, is detached in addition with sow, and factors can cause
Piglet generates oxidative stress.Under oxidative stress situation, steady the weighing property dynamic abnormal of piglet interior free yl makes body free radical produce
Raw excessive or intracellular Antioxidative Defense System is impaired, if cannot repair in time, can induce an illness or cause death.Therefore,
The oxidation resistance of piglet is improved, alleviating oxidative stress is particularly important the damage that it brings.
Invention content
The object of the present invention is to provide a kind of additives improving piglet oxidation resistance.
Additive provided by the invention, active ingredient include vitamin C, folic acid, vitamin A, vitamin D3, vitamin
E, vitamin B2, astragalus polyose, sodium bicarbonate, maltodextrin and glucose.
In above-mentioned additive, active ingredient also can be only made of aforesaid components.
Specifically, in the additive, the percentage composition difference that each component accounts for the additive gross mass is as follows：
Vitamin C：8~12%,
Folic acid：0.1~0.3%,
Vitamin A：0.4~0.9%,
Vitamin D3：2.5~5%,
Vitamin E：4~8%,
Vitamin B2：0.4~0.7%,
Astragalus polyose：5~9%,
Sodium bicarbonate：6~12%,
Maltodextrin：5~12%,
Surplus is glucose.
Vitamin A is in terms of vitamine A acetate, then μ g vitamine A acetates of 1IU vitamin As=0.344；Vitamin E with
Alpha-tocopherol meter, then 1IU vitamin Es=0.668mg alpha-tocopherols.
Specifically, the additive is grouped as by each group of following each mass percentage：
Vitamin C 10%, folic acid 0.2%, vitamin A 0.7%, vitamin D33.5%, vitamin E 6%, vitamin
B20.5%, astragalus polyose 7%, sodium bicarbonate 8%, maltodextrin 10%, glucose 54.1%.
The method for preparing additive that aforementioned present invention provides, includes the following steps：
By aforementioned each component according to proportioning mixing, the additive is obtained.
In addition, application and the additive of the additive of aforementioned present invention offer in livestock drinking-water or feed are as domestic animal
Application in the additive of poultry drinking-water or feed, also belongs to protection scope of the present invention.Wherein, the additive and livestock consumption
The amount ratio of water is 0.8~1.2g：4L, specially 1g：4L, and the amount ratio of the additive and animal feeding-stuff be 0.8~
1.2g：1kg, specially 1g：1kg；
The livestock is pig, specially piglet.
The application for the additive that aforementioned present invention provides, it is specially following 1) to any one in 2)：
1) in the application for improving oxidative stress piglet growth performance；
2) application in the anti-oxidation function for improving piglet.
In addition, using above-mentioned additive provided by the invention to be any one in the product with following function of active ingredient
Kind, also belong to protection scope of the present invention：
3) oxidative stress piglet growth performance is improved；
4) anti-oxidation function of piglet is improved.
Wherein, the amount ratio of the additive and livestock drinking water is 0.8~1.2g：4L, specially 1g：4L, and it is described
The amount ratio of additive and animal feeding-stuff is 08~1.2g：1kg, specially 1g：1kg；
The livestock is pig, specially piglet.
It is described 1) in, it is specially to reduce piglet feed-weight ratio and/or increase piglet day to improve oxidative stress piglet growth performance
Weightening；
It is described 2) in, the anti-oxidation function that improves piglet be specially improve total antioxidant capacity in piglet serum, improve it is young
In Swine serum superoxide dismutase activity (SOD) and reduce piglet serum in Peroxidation Product malonaldehyde (MDA) content in
At least one；
It is described 3) specially to reduce piglet feed-weight ratio and/or increase piglet daily gain；
It is described 4) specially to improve total antioxidant capacity in piglet serum, improve superoxide dismutase work in piglet serum
Power and at least one of the content for reducing Peroxidation Product malonaldehyde in piglet serum.
The present invention is by mixing vitamin, polysaccharide, electrolyte conditioning agent and nutritive carbohydrate simple in structure
After be added in drinking-water or feed, the oxidation resistance of piglet is improved in terms of nutrition adjustment, alleviates the shadow of oxidative stress
It rings, improves the growth performance of piglet.
Specific implementation mode
With reference to specific embodiment, the present invention is further elaborated, but the present invention is not limited to following embodiments.Institute
It is conventional method to state method unless otherwise instructed.The raw material can obtain unless otherwise instructed from open commercial sources.Under
It states content described in embodiment unless otherwise instructed, is mass percentage.
In following embodiments, vitamin A used is Solvisyn-A, is purchased from Royal DSM (China) Co., Ltd, product
Code 5002486, vitamin A content >=500000IU/g, in terms of vitamine A acetate, then the μ of 1IU vitamin As=0.344 g dimensions
Raw element A acetates；
Vitamin D used3For water soluble vitamin D3, be purchased from Royal DSM (China) Co., Ltd, product code 0440140,
Vitamin D3Content >=500000IU/g；
Vitamin E used be watermiscible vitamin E, be purchased from Royal DSM (China) Co., Ltd, product code 5002575,
DL- α-D-α-tocopherol acetate content >=50%；
Astragalus polyose used is purchased from Henan port owned by the government veterinary drug Manufacturing Co., Ltd, and veterinary drug word (2008) 161072710, Radix Astragali is more
Sugared content 70%.
Embodiment 1 supplements influence of the additive to the diquat oxidative stress piglet oxidation resistances induced in drinking-water
Test material
Drinking water additive ingredient is as follows, vitamin C 10%, folic acid 0.2%, vitamin A 0.7%, vitamin D
3.5%, vitamin E 6%, vitamin B20.5%, astragalus polyose 7%, sodium bicarbonate 8%, maltodextrin 10%, glucose
54.1%.
Experimental design and animal
Health " Du × length × big " tri-crossbreeding of 32 21 ages in days is selected in this experiment, close fifty-fifty with male and female by weight
Principle be randomly divided into 4 groups using 2 × 2 factor designs, feed identical diet, 4 groups be respectively oxidative stress+drink into
Light water group, normal+drinking into light water group, oxidative stress+are drunk into supplement additive water group, normal+to drink into supplement additive water
Group each handles 10 repetitions, each repeats 1 pig.Oxidative stress piglet is in the 7th day intraperitoneal injection Diquat (10mg/ of experiment
Kg weight), 21 days experimental periods.The amount ratio of additive and livestock drinking water is 1g:4L.
Test diet
Diet is corn-soybean meal, is prepared with reference to (2012) 7~11kg pig nutritional requirements of NRC.Diet forms and nutrition
Level is shown in Table 1.
Table 1, diet composition and trophic level
1Every kilogram of mixed feed is provided：VA, 9000IU；VD3, 3000IU；VE, 20.0IU；VK3, 3.0mg；VB1,
1.5mg；VB2, 4.0mg；VB6, 3.0mg；VB12, 0.2mg；Niacin, 30.0mg；Pantothenic acid, 15.0mg；Folic acid, 0.75mg；Biology
Element, 0.1mg；
Wherein, vitamin A is in terms of vitamine A acetate, then the μ g vitamine A acetates of 1IU vitamin As=0.344；
Vitamin E is in terms of alpha-tocopherol, then 1IU vitamin e1s=0.668mg alpha-tocopherols；
2Every kilogram of mixed feed is provided：Fe, 96.0mg；Cu, 5.6mg；Zn, 93.0mg；Mn, 3.7mg；I, 0.13mg；
Se, 0.28mg.
Popcorn is purchased from Zhengzhou good luck year feed corporation,Ltd, product standard number in formula：Q/XPS004-2005；It is swollen
Change soybean purchased from extra large (Guanghan) the grain and oil feed corporation,Ltd product standard number of benefit：Q/79180615-7.11-2012；Low albumin milk
Clear powder is purchased from clarke Ta Lisi nutraceutical group (China) Co., Ltd, commodity bar code：0190052；Soy protein concentrate
Purchased from beneficial extra large (Port of Fangcheng) soy industry Co., Ltd, product standard number：Q/YHS 01；Soya-bean oil is raised purchased from extra large (Guanghan) grain and oil of benefit
Expect Co., Ltd, product standard number：GB1535；Mountain flour is purchased from Sichuan Province Baoxing County Heng Tong calcium carbonate factory, executes standard：DB/
T511827-001-2005；Choline chloride is purchased from Yixing Temin feed addictive Co., Ltd of section, product standard number：HG/
T2941-2004,50% refers to the effective content of choline in raw material choline chloride, and 0.10 is the content of raw material choline chloride.Hereafter such as
Without special instruction, the above raw material corresponds to company at it and buys.
Feeding management
Certain year April, experiment are carried out in Sichuan Agricultural University's Animal nutrition research institute teaching and scientific research proving ground, test pig
Single cage is raised.During experiment, test pig free water, daily feeding 4 times (08:00,12:00,16:00,20:00), few to feed frequently
Add, feeds amount showed a small surplus in hopper as degree.Pig house gives up temperature control system at 27~28 DEG C, and relative humidity is controlled 80% or so.It is fixed
Phase rinses colony house, keeps colony house ventilation, cleaning, dry, health.
Index determining
(1) growth performance
Average daily gain：Respectively in experiment starting, terminate 8 points of morning piglet weigh on an empty stomach, calculating averagely increases day by day
Weight.
Average daily gain：The daily every feed intake of accurate recording, calculates average daily gain during experiment.
Feed-weight ratio：Feed-weight ratio is calculated according to average daily gain and average daily gain.
(2) total antioxidant capacity, the measurement of SOD activity and MDA contents in blood
Empty stomach blood sampling is carried out to all piglets, serum is detached, biology is built up using Nanjing the morning on the day of off-test
The detection kit of Graduate School of Engineering is measured total antioxidant capacity in serum, SOD activity, MDA contents, determination step and knot
Fruit calculates and carries out to specifications.
Data statistics and analysis
Test data is arranged using Microsoft Excel 2007, and all data use in SAS8.0 statistical softwares
General linear model (GLM) program to data carry out 2 × 2 liang of factor variance analyses, be used in combination DuncanShi methods progress multiple ratio
Compared with each index is counted as unit of each repetition.With P<0.05 is significant difference criterion, with P≤0.10 for difference trend
Criterion.All data are that " mean+SD " indicates.
As a result
As shown in Table 2, it compared with drinking light water group piglet, drinks and does not significantly affect piglet into supplement additive water and be averaged day
Weightening and average daily gain, but significantly reduce the feed-weight ratio (P of piglet<0.05), and oxidative stress baby pig feedstuff can be alleviated
Again than influence (P<0.05) growth performance, is improved.
As shown in Table 3, compared with drinking light water group piglet, the water drunk into supplement additive does not significantly affect serum MDA
Generation, but considerably improve piglet soluble transferring receptor and SOD vigor (P<0.05), and alleviation oxidative stress draws
The trend (P=0.10) that the piglet Content of MDA risen improves, and then improve the oxidation resistance of piglet.
Table 2 supplements influence of the additive to oxidative stress piglet growth performance by drinking-water
Note：a,b,cDifferent letters of going together indicate significant difference (P<0.05).
Table 3 supplements influence of the additive to oxidative stress piglet antioxygenic property by drinking-water
Note：a,b,cDifferent letters of going together indicate significant difference (P<0.05).
Embodiment 2 supplements influence of the additive to weanling pig oxidation resistance in drinking-water
Test material
Drinking water additive ingredient is as follows, vitamin C 10%, folic acid 0.2%, vitamin A 0.7%, vitamin D
3.5%, vitamin E 6%, vitamin B20.5%, astragalus polyose 7%, sodium bicarbonate 8%, maltodextrin 10%, glucose
54.1%.
Experimental design and animal
This experiment selects health " Du × length × big " ternary for the 28 ages in days wean that 560 average weights are about 7.5kg miscellaneous
It hands over piglet to be randomly divided into 4 groups by principle similar in weight, fed identical diet, but gives the (control of common water respectively
Group) and be added to the water of various dose drinking water additive (additive capacity is respectively 0.8g:4L、1g:4L and 1.2g:4L), each
7 repetitions of group, each repeat 20 piglets.31 days experimental periods.
Test diet
Experiment diet be corn-soybean meal, provided for test pig place, nutritional ingredient meet (2012) 7~11kg of NRC with
11~25kg pig nutritional requirements.
Feeding management
In certain year May, experiment is carried out in Zhengzhou, henan large―scale hoggery, and during experiment, test pig is freely eaten and drinking-water.Pig
At 25~27 DEG C, relative humidity is controlled 70% or so house house temperature control system.Keep colony house ventilation, cleaning, dry, health.
Index determining
The measurement of total antioxidant capacity in blood
Both ends pig is randomly selected to each repetition and carries out empty stomach blood sampling, serum is detached, adopts the morning on the day of off-test
The detection kit that Bioengineering Research Institute is built up with Nanjing is measured total antioxidant capacity in serum, determination step and result
Calculating carries out to specifications.
Data statistics and analysis
Test data is arranged using Microsoft Excel 2007, using single factor test variance in 8.0 softwares of SAS
Analysis carries out DuncanShi Multiple range tests when significant difference.Each index is counted as unit of each repetition, with P<0.05 is difference
Notable criterion, with P≤0.10 for difference Trend judgement standard.All data are indicated with " average value ± standard error ".
As a result
As shown in Table 4, compared with the control group, the supplement of drinking water additive significantly improves the total antioxidant capacity of piglet, and
It is gradually increased with the raising oxidation resistance of additive adding proportion, shows that the supplement of drinking water additive improves to a certain extent
The oxidation resistance of piglet.And in view of the double factor of effect (influence to oxidation resistance) and cost, drinking-water addition
Agent is with water with 1g：The adding proportion of 4L is best.
Influence of the supplement additive to weanling pig oxidation resistance in table 4, drinking-water
Embodiment 3 supplements influence of the additive to piglet oxidation resistance in feed
Test material
Additive component is as follows, vitamin C 10%, folic acid 0.2%, vitamin A 0.7%, vitamin D 3.5%, dimension
Raw element E 6%, vitamin B20.5%, astragalus polyose 7%, sodium bicarbonate 8%, maltodextrin 10%, glucose 54.1%.
Experimental design and animal
This experiment selects health " Du × length × big " three way cross piglet that 40 average weights are about 21kg, by weight phase
Close principle is randomly divided into 4 groups, respectively common diet group (control group) and the additive diet group for being added to various dose
(additive capacity is respectively 0.8g:1kg、1g:1kg and 1.2g:1kg), each 10 repetitions of group, each repeat 1 piglet.Experiment
14 days phases.
Test diet
Diet is corn-soybean meal, is prepared with reference to (2012) 11~25kg pig nutritional requirements of NRC.Diet forms and battalion
Foster level is shown in Table 5.
Table 5, diet composition and trophic level
1) vitamin premix provides The vitamin premix provides for every kilogram of diet
following per kilogram of diet：VA, 20000IU；VD3,500IU；VE, 12.5IU；VK3,2.5mg；VB1,
1.0mg；VB2,8.0mg；VB6,3.0mg；VB12,0.015mg；Niacin, 17.5mg；Pantothenic acid, 12.5mg；Folic acid, 0.25mg；
Wherein, vitamin A is in terms of vitamine A acetate, then the μ g vitamine A acetates of 1IU vitamin As=0.344；
Vitamin E is in terms of alpha-tocopherol, then 1IU vitamin e1s=0.668mg alpha-tocopherols；
2) mineral substance premix can provide The mineral premix provides for every kilogram of diet
following per kilogram of diet：Fe 100mg, Cu 6mg, Zn100mg, Mn 4mg, I 0.3mg, Se
0.35mg。
Feeding management
Certain year June, experiment are carried out in Sichuan Agricultural University's Animal nutrition research institute teaching and scientific research proving ground, test pig
Single cage is raised.During experiment, test pig free water, daily feeding 4 times (08:00,12:00,16:00,20:00), few to feed frequently
Add, feeds amount showed a small surplus in hopper as degree.Pig house gives up temperature control system at 27~28 DEG C, and relative humidity is controlled 80% or so.It is fixed
Phase rinses colony house, keeps colony house ventilation, cleaning, dry, health.
Index determining
(1) growth performance：
Average daily gain：Respectively in experiment starting, terminate 8 points of morning piglet weigh on an empty stomach, calculating averagely increases day by day
Weight.
Average daily gain：The daily every feed intake of accurate recording, calculates average daily gain during experiment.
Feed-weight ratio：Feed-weight ratio is calculated according to average daily gain and average daily gain.
(2) total antioxidant capacity, the measurement of SOD activity and MDA contents in blood
Empty stomach blood sampling is carried out to all piglets, serum is detached, biology is built up using Nanjing the morning on the day of off-test
The detection kit of Graduate School of Engineering be measured total antioxidant capacity in serum, SOD activity and MDA contents, determination step and
As a result it calculates and carries out to specifications.
Data statistics and analysis
Test data is arranged using Microsoft Excel 2007, is examined using the t of 8.0 softwares of SAS and is carried out 2
The analysis of test data between a group, each index are counted as unit of each repetition.With P<0.05 is significant difference criterion, with
P≤0.10 is difference Trend judgement standard.All data are indicated with " average value ± standard error ".
As a result
As shown in Table 6, compared with the control group, in feed supplemented with additive do not significantly affect piglet average daily gain and
Average feedstuff-meat ratio, but as the raising of additive capacity significantly improves piglet daily gain (P<0.05).Improve the growth of piglet
Performance.
As shown in Table 7, compared with the control group, SOD activity in piglet serum is not significantly affected supplemented with additive in feed
With MDA contents, but as the raising of additive capacity significantly improves piglet total antioxidant capacity (P<0.05).
And in view of the double factor of effect (influence to oxidation resistance and growth performance) and cost, drinking water additive
With water with 1g：The adding proportion of 1kg is best.
Influence of the additive to piglet growth performance is supplemented in table 6, feed
Note：a,b,cDifferent letters of going together indicate significant difference (P<0.05).
Influence of the additive to piglet anti-oxidation function is supplemented in table 7, feed
Note：a,b,cDifferent letters of going together indicate significant difference (P<0.05).
Analysis
By the result of above three experiment it is found that supplement additive can improve the anti-oxidant of piglet in feed or drinking-water
Ability, and then improve the growth performance of piglet.
Conclusion
Test result shows that in 4L drinks water, the supplement 1g additives are equal in supplement 1g additives or 1kg animal feeding-stuffs
The oxidation resistance that piglet can be improved improves piglet growth performance so as to improve the general level of the health of piglet.
Claims (4)
1. a kind of application of additive in animal feeding-stuff, the additive is grouped by each group of following each mass percentage
At：
Vitamin C 10%, folic acid 0.2%, vitamin A 0.7%, vitamin D33.5%, vitamin E 6%, vitamin B20.5%,
Astragalus polyose 7%, sodium bicarbonate 8%, maltodextrin 10%, glucose 54.1%.
2. a kind of application of additive in the additive as animal feeding-stuff, the additive is by following each mass percentage
Each group be grouped as：
Vitamin C 10%, folic acid 0.2%, vitamin A 0.7%, vitamin D33.5%, vitamin E 6%, vitamin B20.5%,
Astragalus polyose 7%, sodium bicarbonate 8%, maltodextrin 10%, glucose 54.1%.
3. application according to claim 1, it is characterised in that：The amount ratio of the additive and animal feeding-stuff be 0.8~
1.2g：1kg；
The livestock is pig.
4. application according to claim 3, which is characterized in that the amount ratio of the additive and animal feeding-stuff is 1g：
1kg。
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| CN101669578A (en) * | 2009-09-24 | 2010-03-17 | 北京福乐维生物技术有限公司 | High efficiency livestock and poultry nutrition replenisher and preparation method thereof |
| CN104161185A (en) * | 2013-07-29 | 2014-11-26 | 河南联合英伟饲料有限公司 | Nutritional feed additive for livestock and poultry, preparation method thereof and application thereof |
| CN104970205A (en) * | 2015-05-29 | 2015-10-14 | 四川农业大学 | Drinking water additive for improving utilization of nutrients and immune function of weaned pigs |
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| US4818543A (en) * | 1987-10-15 | 1989-04-04 | University Of Georgia Research Foundation, Inc. | Control of food intake in poultry by dietary addition |
| CN101669578A (en) * | 2009-09-24 | 2010-03-17 | 北京福乐维生物技术有限公司 | High efficiency livestock and poultry nutrition replenisher and preparation method thereof |
| CN104161185A (en) * | 2013-07-29 | 2014-11-26 | 河南联合英伟饲料有限公司 | Nutritional feed additive for livestock and poultry, preparation method thereof and application thereof |
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