CN105061618A - Ultrasonic extraction process of saccharum officinarum polysaccharide - Google Patents
Ultrasonic extraction process of saccharum officinarum polysaccharide Download PDFInfo
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- CN105061618A CN105061618A CN201510477890.XA CN201510477890A CN105061618A CN 105061618 A CN105061618 A CN 105061618A CN 201510477890 A CN201510477890 A CN 201510477890A CN 105061618 A CN105061618 A CN 105061618A
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Abstract
The invention discloses an ultrasonic extraction process of saccharum officinarum polysaccharide. The process includes: firstly subjecting saccharum officinarum residue to microwave vacuum drying treatment, then crushing the saccharum officinarum residue, adding water into the saccharum officinarum residue powder and stirring the mixture evenly, then adding acid to adjust the pH value, then performing ultrasonic treatment, decocting the ultrasonic treatment liquid, and performing filtering to obtain a water extract, sending the water extract into an ultrafiltration device to conduct ultrafiltration treatment, then sending the ultrafiltrate into a centrifugal machine to conduct centrifugal treatment, concentrating the centrifugate to obtain a concentrated solution, adding ethanol into the concentrated solution and conducting standing, then carrying out centrifugal separation, and drying the obtained saccharum officinarum polysaccharide solution, thus obtaining saccharum officinarum polysaccharide. By adopting the microwave low temperature vacuum drying and ultrasonic wave synergistic treatment technology, the process provided by the invention ensures effective wall breaking, significantly improves the polysaccharide yield and antioxidant activity, and greatly enhances the extraction rate of saccharum officinarum polysaccharide.
Description
Technical field
The present invention relates to sugared technology field, specifically, relate to a kind of ultrasonic extraction process of sugarcane polysaccharide.
Background technology
Bagasse is the principal by product of sugar industry, is major portion remaining after sugarcane mechanical compaction receives sugar, belongs to the one in agricultural solid residue.China has multiple province to grow cane, and each sugar refinery produces a large amount of bagasse, finds suitable approach comprehensive utilization bagasse significant.Research shows that the polysaccharide extracted from bagasse has the effect such as antitumor, antiviral, hypoglycemic, reducing blood-fat, enhancing immunity, and from bagasse, extract that polysaccharide develops and utilizes is a kind of effective way.The extracting method of existing polysaccharide has water extraction and alcohol precipitation method, microwave method, acid-base method, ferment treatment method etc., and the extracting method such as microwave method have certain effect to the extraction yield improving polysaccharide, but higher to equipment requirements, implement to acquire a certain degree of difficulty; Though acid or alkali method can improve extraction yield, acid or alkali can destroy the structure of polysaccharide.The multiplex water extraction and alcohol precipitation method of extraction of current bagasse polysaccharide, but the polysaccharide content obtained is lower, needs to be further purified.Ferment treatment method have reaction temperature and, the advantage such as yield is high and easy to operate, but bagasse is primarily of compositions such as holocellulose, xylogen and pentosan, have more tough hard ancient piece of jade, round, flat and with a hole in its centre tissue, the polysaccharide yield of traditional enzymolysis process is on the low side, and the polysaccharide anti-oxidative ability of preparation is low.
Summary of the invention
In view of this, the object of this invention is to provide a kind of ultrasonic extraction process of sugarcane polysaccharide, to solve prior art, to prepare polysaccharide yield on the low side, and the low and deficiency that exists of the polysaccharide anti-oxidative ability of preparation.
The present invention solves the problems of the technologies described above by the following technical programs:
A ultrasonic extraction process for sugarcane polysaccharide, comprises the following steps:
(1) pre-treatment: first bagasse is used microwave vacuum dryer drying treatment, then bagasse powder being broken to fineness is 60 ~ 200 object fine powders, obtains cane powder;
(2) supersound process: cane powder is added water after stirring, the pH value adding acid-conditioning solution is again 6 ~ 6.5, then send in ultrasonic device, ultrasonic power be 40 ~ 80KHz, temperature be the condition of 60 ~ 80 DEG C under process 8 ~ 20min, obtain sonification fluid;
(3) decoct: sonification fluid is decocted 100 ~ 120min, and filter to obtain Aqueous extracts, filter residue adds 6 times of soak by water 50 ~ 70min, filters to obtain Aqueous extracts, merges twice Aqueous extracts;
(4) removal of impurities: Aqueous extracts is sent in ultrafiltration apparatus and carry out uf processing, obtain ultrafiltrated after removing impurity, ultrafiltrated is squeezed in whizzer, centrifugation 8 ~ 16min under the rotating speed of 1600 ~ 2000 revs/min, further removing impurity, obtains centrifugate;
(5) concentrated: by centrifugate vacuum tightness be-0.08MPa, temperature is concentrated into soluble solid >=15% under being the condition of 40 ~ 60 DEG C, obtains concentrated solution;
(6) alcohol precipitation: add ethanol in concentrated solution, makes the alcohol concn of solution be 84 ~ 95%, and after leaving standstill 2 ~ 3h, under the rotating speed of 1600 ~ 2000 revs/min, centrifugation 8 ~ 16min, obtains sugarcane polysaccharide liquid, sugarcane polysaccharide liquid is dried, obtains sugarcane polysaccharide.
In described preprocessing process, the pressure controlling microwave vacuum dryer during bagasse drying treatment is 12 ~ 24KPa, temperature is 40 ~ 60 DEG C.
In described ultrasound treatment step, cane powder is added after 8 times of water stir, then the pH value adding acid-conditioning solution is 6.2, then sends in microwave dryer, ultrasonic power be 60KHz, temperature processes 16min under being the condition of 70 DEG C.
In described alcohol precipitation process, the bake out temperature of sugarcane polysaccharide liquid is 40 ~ 80 DEG C.
Beneficial effect of the present invention is: the present invention destroys albumen and the immobilized artificial membrane of polysaccharide outer layer covers by microwave oscillation, promote the stripping of polysaccharide, significantly improve the extraction yield of polysaccharide, and at lower vacuum state, drying temperature can be controlled in lower temperature, the resistance of oxidation of the polysaccharide that minimizing high temperature and oxygen cause declines; By carrying out supersound process again by after cane powder enzymolysis, utilize ultrasonic cavitation effect, heat effect and mechanical effect effect, when the ul-trasonic irradiation of macro-energy is in cane powder, this cane powder is torn into many little holes, these little holes are instantaneous closed, produce up to several thousand atmospheric instantaneous pressure, cell walls in cane powder and organism are being broken instantaneously, substantially reduce the extraction time of sugarcane polysaccharide, hyperacoustic oscillating action strengthens the release that endocellular sugar divides, sugar is extracted, thus improves the extraction yield of sugarcane polysaccharide.To sum up, the present invention, by adopting microwave low-temperature vacuum drying and ultrasonic synergistic treatment process, guarantees effective broken wall, significantly improves yield and the anti-oxidant activity of polysaccharide, greatly improves the extraction yield of sugarcane polysaccharide.
Embodiment
Conveniently those skilled in the art will recognize that the present invention will be further described below in conjunction with embodiment.Embodiment is only illustrating this invention, is not limitation of the invention, and the step not doing in embodiment to illustrate is all prior arts, is not described in detail at this.
Embodiment one
A ultrasonic extraction process for sugarcane polysaccharide, comprises the following steps:
(1) pre-treatment: first bagasse is sent in microwave vacuum dryer, pressure be 12KPa, temperature is the condition drying treatment of 40 DEG C, then bagasse powder being broken to fineness is 60 object fine powders, obtains cane powder;
(2) supersound process: cane powder is added after 8 times of water stir, then the pH value adding acid-conditioning solution is 6, then sends in ultrasonic device, ultrasonic power be 40KHz, temperature processes 8min under being the condition of 60 DEG C, obtains sonification fluid;
(3) decoct: sonification fluid is decocted 100min, filters to obtain Aqueous extracts, filter residue adds 6 times of soak by water 50min, filters to obtain Aqueous extracts, merges twice Aqueous extracts;
(4) removal of impurities: Aqueous extracts is sent in ultrafiltration apparatus and carry out uf processing, obtain ultrafiltrated after removing impurity, squeezed into by ultrafiltrated in whizzer, under the rotating speed of 1600 revs/min, centrifugation 8min, removes impurity further, obtains centrifugate;
(5) concentrated: by centrifugate vacuum tightness be-0.08MPa, to be concentrated into soluble solid under being the condition of 40 DEG C be 15% to temperature, obtains concentrated solution;
(6) alcohol precipitation: add ethanol in concentrated solution, makes the alcohol concn of solution be 84%, and after leaving standstill 2h, under the rotating speed of 1600 revs/min, centrifugation 8min, obtains sugarcane polysaccharide liquid, sugarcane polysaccharide liquid is dried at the temperature of 40 DEG C, obtains sugarcane polysaccharide.
Adopt above-mentioned process, the leaching content of sugarcane polysaccharide is 87g/kg, and the resistance of oxidation of sugarcane polysaccharide is 82.7%.
Embodiment two
A ultrasonic extraction process for sugarcane polysaccharide, comprises the following steps:
(1) pre-treatment: first bagasse is sent in microwave vacuum dryer, pressure be 24KPa, temperature is the condition drying treatment of 60 DEG C, then bagasse powder being broken to fineness is 200 object fine powders, obtains cane powder;
(2) supersound process: cane powder is added after 8 times of water stir, then the pH value adding acid-conditioning solution is 6.5, then sends in ultrasonic device, ultrasonic power be 80KHz, temperature processes 20min under being the condition of 80 DEG C, obtains sonification fluid;
(3) decoct: sonification fluid is decocted 120min, filters to obtain Aqueous extracts, filter residue adds 6 times of soak by water 70min, filters to obtain Aqueous extracts, merges twice Aqueous extracts;
(4) removal of impurities: Aqueous extracts is sent in ultrafiltration apparatus and carry out uf processing, obtain ultrafiltrated after removing impurity, squeezed into by ultrafiltrated in whizzer, under the rotating speed of 2000 revs/min, centrifugation 16min, removes impurity further, obtains centrifugate;
(5) concentrated: by centrifugate vacuum tightness be-0.08MPa, to be concentrated into soluble solid under being the condition of 60 DEG C be 15% to temperature, obtains concentrated solution;
(6) alcohol precipitation: add ethanol in concentrated solution, makes the alcohol concn of solution be 95%, and after leaving standstill 3h, under the rotating speed of 2000 revs/min, centrifugation 16min, obtains sugarcane polysaccharide liquid, sugarcane polysaccharide liquid is dried at the temperature of 80 DEG C, obtains sugarcane polysaccharide.
Adopt above-mentioned process, the leaching content of sugarcane polysaccharide is 88g/kg, and the resistance of oxidation of sugarcane polysaccharide is 82.1%.
Embodiment three
A ultrasonic extraction process for sugarcane polysaccharide, comprises the following steps:
(1) pre-treatment: first bagasse is sent in microwave vacuum dryer, pressure be 16KPa, temperature is the condition drying treatment of 50 DEG C, then bagasse powder being broken to fineness is 80 object fine powders, obtains cane powder;
(2) supersound process: cane powder is added after 8 times of water stir, then the pH value adding acid-conditioning solution is 6.2, then sends in ultrasonic device, ultrasonic power be 60KHz, temperature processes 16min under being the condition of 70 DEG C, obtains sonification fluid;
(3) decoct: sonification fluid is decocted 110min, filters to obtain Aqueous extracts, filter residue adds 6 times of soak by water 60min, filters to obtain Aqueous extracts, merges twice Aqueous extracts;
(4) removal of impurities: Aqueous extracts is sent in ultrafiltration apparatus and carry out uf processing, obtain ultrafiltrated after removing impurity, squeezed into by ultrafiltrated in whizzer, under the rotating speed of 1800 revs/min, centrifugation 12min, removes impurity further, obtains centrifugate;
(5) concentrated: by centrifugate vacuum tightness be-0.08MPa, to be concentrated into soluble solid under being the condition of 50 DEG C be 16% to temperature, obtains concentrated solution;
(6) alcohol precipitation: add ethanol in concentrated solution, makes the alcohol concn of solution be 90%, and after leaving standstill 2.6h, under the rotating speed of 1800 revs/min, centrifugation 12min, obtains sugarcane polysaccharide liquid, sugarcane polysaccharide liquid is dried at the temperature of 60 DEG C, obtains sugarcane polysaccharide.
Adopt above-mentioned process, the leaching content of sugarcane polysaccharide is 94g/kg, and the resistance of oxidation of sugarcane polysaccharide is 83.2%.
Claims (4)
1. a ultrasonic extraction process for sugarcane polysaccharide, is characterized in that: comprise the following steps:
(1) pre-treatment: first bagasse is used microwave vacuum dryer drying treatment, then bagasse powder being broken to fineness is 60 ~ 200 object fine powders, obtains cane powder;
(2) supersound process: cane powder is added water after stirring, the pH value adding acid-conditioning solution is again 6 ~ 6.5, then send in ultrasonic device, ultrasonic power be 40 ~ 80KHz, temperature be the condition of 60 ~ 80 DEG C under process 8 ~ 20min, obtain sonification fluid;
(3) decoct: sonification fluid is decocted 100 ~ 120min, and filter to obtain Aqueous extracts, filter residue adds 6 times of soak by water 50 ~ 70min, filters to obtain Aqueous extracts, merges twice Aqueous extracts;
(4) removal of impurities: Aqueous extracts is sent in ultrafiltration apparatus and carry out uf processing, obtain ultrafiltrated after removing impurity, ultrafiltrated is squeezed in whizzer, centrifugation 8 ~ 16min under the rotating speed of 1600 ~ 2000 revs/min, further removing impurity, obtains centrifugate;
(5) concentrated: by centrifugate vacuum tightness be-0.08MPa, temperature is concentrated into soluble solid >=15% under being the condition of 40 ~ 60 DEG C, obtains concentrated solution;
(6) alcohol precipitation: add ethanol in concentrated solution, makes the alcohol concn of solution be 84 ~ 95%, and after leaving standstill 2 ~ 3h, under the rotating speed of 1600 ~ 2000 revs/min, centrifugation 8 ~ 16min, obtains sugarcane polysaccharide liquid, sugarcane polysaccharide liquid is dried, obtains sugarcane polysaccharide.
2. the ultrasonic extraction process of sugarcane polysaccharide as claimed in claim 1, is characterized in that: in described preprocessing process, and the pressure controlling microwave vacuum dryer during bagasse drying treatment is 12 ~ 24KPa, temperature is 40 ~ 60 DEG C.
3. the ultrasonic extraction process of sugarcane polysaccharide as claimed in claim 1, it is characterized in that: in described ultrasound treatment step, cane powder is added after 8 times of water stir, the pH value adding acid-conditioning solution is again 6.2, then send in microwave dryer, ultrasonic power be 60KHz, temperature processes 16min under being the condition of 70 DEG C.
4. the ultrasonic extraction process of sugarcane polysaccharide as claimed in claim 1, it is characterized in that: in described alcohol precipitation process, the bake out temperature of sugarcane polysaccharide liquid is 40 ~ 80 DEG C.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105399851A (en) * | 2015-12-09 | 2016-03-16 | 重庆三零三科技有限公司 | Extraction method of sugarcane leaf polysaccharide |
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| CN102911286A (en) * | 2012-11-22 | 2013-02-06 | 广西中医药大学 | Preparation method of sugarcane leaf polysaccharides |
| CN103626887A (en) * | 2013-12-02 | 2014-03-12 | 广西壮族自治区农业科学院农产品加工研究所 | Method for preparing sugarcane top polysaccharide from sugarcane tops |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101392032A (en) * | 2008-11-05 | 2009-03-25 | 中国林业科学研究院林产化学工业研究所 | Method for preparing bamboo leaf polysaccharide extract by member separation |
| CN101712727A (en) * | 2009-11-24 | 2010-05-26 | 中国计量学院 | Method for extracting black fungus polysaccharide |
| CN102336841A (en) * | 2011-10-18 | 2012-02-01 | 中国计量学院 | Method for extracting tea polysaccharide |
| CN102504938A (en) * | 2011-11-08 | 2012-06-20 | 浙江省林业科学研究院 | Cold pressing and physical refining process of camellia oil |
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| CN103626887A (en) * | 2013-12-02 | 2014-03-12 | 广西壮族自治区农业科学院农产品加工研究所 | Method for preparing sugarcane top polysaccharide from sugarcane tops |
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Effective date of registration: 20190926 Address after: 538101 Jiangping Zhenjiang Ping Industrial Park, Dongxing City, Fangchenggang City, Guangxi Zhuang Autonomous Region Patentee after: GUANGXI DONGXING DINGKANG PLASTIC INDUSTRY CO., LTD. Address before: 530012 Liyuan, No. 12 Minority Avenue, Qingxiu District, Nanning City, Guangxi Zhuang Autonomous Region. Tianjin A1310 Patentee before: GUANGXI NANNING PAITENG TECHNOLOGY CO., LTD. |