CN105399851A - Extraction method of sugarcane leaf polysaccharide - Google Patents
Extraction method of sugarcane leaf polysaccharide Download PDFInfo
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- CN105399851A CN105399851A CN201510903017.2A CN201510903017A CN105399851A CN 105399851 A CN105399851 A CN 105399851A CN 201510903017 A CN201510903017 A CN 201510903017A CN 105399851 A CN105399851 A CN 105399851A
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- sacchari sinensis
- caulis sacchari
- polysaccharide
- sinensis leaf
- alcohol precipitation
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Sustainable Development (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an extraction method of sugarcane leaf polysaccharide. The method includes: taking commercially available sugarcane leaves as the raw materials, and carrying out cleaning, low temperature rehydration, cold pulping, enzymolysis, separation, decolorization, ultrafiltration, alcohol precipitation and separation to obtain a precipitate, i.e. crude polysaccharide; and subjecting the crude polysaccharide to water dissolving, degreasing, deproteinization, decolorization, alcohol precipitation, freeze drying and crushing to obtain sugarcane leaf polysaccharide. The method provided by the invention carries out effective biological extraction on sugarcane leaves, combines the traditional polysaccharide extraction technology with the advanced bioengineering technology, has advanced process, reduces energy consumption, and provides an effective way for deep processing of sugarcane leaves.
Description
Technical field
The present invention relates to deep processing of farm products technology, relate to a kind of extracting method of Caulis Sacchari sinensis leaf polysaccharide especially.
Background technology
Polysaccharide and protein, gene are three large fields of life science, the research of latter two has obtained huge achievement in 20th century, and become 21 century life science Disciplinary Frontiers as the polysaccharide that life can be made to shine extraordinary splendor, the extensive concern having caused countries in the world is developed to it.Polyose is also known as saccharan (Polysaccharides), molecular weight is at tens of thousands or millions of, vegetable polysaccharides comprises starch, Mierocrystalline cellulose, dextran (as lentinan), Polylevulosan, natural gum, phlegmatic temperament, viscose glue fat (as agar) etc., and wherein some immuno-stimulating polysaccharide is inhibition of cancer cell agent.
Current domestic vegetable polysaccharides is various in style, as lentinan, astragalus polysaccharides, laminarin, squash polyoses, Aloe polysaccharide etc., go deep into along with to the bioactive research of polysaccharide, the biological activity mechanism of polysaccharide, efficacy factor can definitely, and its Application Areas also will be widened more.
Caulis Sacchari sinensis leaf is the leaf of grass sugarcane.Sugarcane is extensively planted in tropical and subtropical region, and Sugarcane Fields of China district is mainly distributed in the ground such as Guangxi, Guangdong, Taiwan.Caulis Sacchari sinensis leaf is as the byproduct after sugarcane good harvest, and annual output is quite high, is but underutilized, and except small part is processed to animal-feed, papermaking etc., major part is burned by sugarcane grower, causes the wasting of resources, environmental pollution and easily cause hill fire.Caulis Sacchari sinensis leaf contains the materials such as a large amount of chlorophyll, amino acid, polysaccharide profit flavonoid, and its tender leaf and the sugarcane tip, containing materials such as peroxidase, have the pharmacological actions such as anti-oxidant, antibacterial, hypoglycemic, antitumor.Actually rare to the research of Caulis Sacchari sinensis leaf polysaccharide both at home and abroad, the only report such as extraction process, Pharmacodynamics screening.Prepare polysaccharide many employings water extraction and alcohol precipitation method at present, Aqueous extracts, when alcohol settling process, removes in precipitation containing polysaccharose substance, also containing the impurity such as protein, pigment, affect the purity of polysaccharide, therefore need the relative substance such as protein in removing polysaccharide, improve the purity of polysaccharide.
The present inventor take Caulis Sacchari sinensis leaf as raw material, probe into the extraction of Caulis Sacchari sinensis leaf polysaccharide, purification process, explore the preparation technology of the high-purity Caulis Sacchari sinensis leaf polysaccharide of high yield, for digging utilization, the deep development of sugarcane waste material, promote that Sugarcane Industry development has positive effect further.
Summary of the invention
For making full use of Caulis Sacchari sinensis leaf resource, the invention provides a kind of extracting method of Caulis Sacchari sinensis leaf polysaccharide.Concrete steps are as follows:
1) preparation of Crude polysaccharides: Caulis Sacchari sinensis leaf raw material obtains Caulis Sacchari sinensis leaf slag and Caulis Sacchari sinensis leaf juice respectively through cleaning, low temperature rehydration, cold making beating, enzymolysis, separation, Caulis Sacchari sinensis leaf juice through decolouring, ultrafiltration obtains permeate and trapped fluid, trapped fluid through alcohol precipitation, be separated that to be precipitated thing be Crude polysaccharides;
2) purifying of Caulis Sacchari sinensis leaf polysaccharide: after water-soluble, degreasing, deproteinated, decolouring, alcohol precipitation, lyophilize, pulverizing, Caulis Sacchari sinensis leaf polysaccharide is obtained to the process of Crude polysaccharides;
(1) deionized water dissolving, degreasing
By Crude polysaccharides deionized water dissolving, adopt ethanol, ether hybrid system removing lipoid material wherein;
(2) deproteinated
Adopt trichoroacetic acid(TCA) removing protein wherein;
(3) decolour
Polysaccharide soln color after degreasing, deproteinated is light yellow, and adopt activated carbon decolorizing technology, make polysaccharide soln reach water white transparency, activated carbon dosage is 0.7%, bleaching temperature 60 DEG C, bleaching time 1 hour;
(4) alcohol precipitation
After decolouring, activated carbon filtration is fallen, adopt 95% ethanol by Caulis Sacchari sinensis leaf polysaccharide precipitation out;
(5) lyophilize
Adopt Vacuum Freezing & Drying Technology that the moisture content of Caulis Sacchari sinensis leaf polysaccharide is reduced to less than 5%, Vacuum Freezing & Drying Technology at utmost can keep the activity of polysaccharide;
(6) pulverize
Polysaccharide after freeze-drying is pulverized;
The preparation of described Crude polysaccharides comprises: Caulis Sacchari sinensis leaf raw material obtains Caulis Sacchari sinensis leaf slag and Caulis Sacchari sinensis leaf juice respectively through cleaning, rehydration, making beating, enzymolysis, separation, Caulis Sacchari sinensis leaf juice through decolouring, ultrafiltration obtains permeate and trapped fluid, trapped fluid through alcohol precipitation, be separated that to be precipitated thing be Crude polysaccharides.
The extracting method of a kind of Caulis Sacchari sinensis leaf polysaccharide of the present invention, by carrying out the biological extraction of effective effect to Caulis Sacchari sinensis leaf, polysaccharide technology tradition extracted combines with advanced biotechnology, technique is advanced, energy consumption reduces, product reaches more than 90% containing polysaccharide, and the intensive processing for Caulis Sacchari sinensis leaf provides effective approach.
Claims (4)
1. an extracting method for Caulis Sacchari sinensis leaf polysaccharide, is characterized in that, comprises the following steps:
(1) preparation of Crude polysaccharides: Caulis Sacchari sinensis leaf raw material obtains Caulis Sacchari sinensis leaf slag and Caulis Sacchari sinensis leaf juice respectively through cleaning, low temperature rehydration, cold making beating, enzymolysis, separation, Caulis Sacchari sinensis leaf juice through decolouring, ultrafiltration obtains permeate and trapped fluid, trapped fluid through alcohol precipitation, be separated that to be precipitated thing be Crude polysaccharides;
(2) purifying of Caulis Sacchari sinensis leaf polysaccharide: after water-soluble, degreasing, deproteinated, decolouring, alcohol precipitation, lyophilize, pulverizing, Caulis Sacchari sinensis leaf polysaccharide is obtained to the process of Crude polysaccharides;
(3) deionized water dissolving, degreasing
By Crude polysaccharides deionized water dissolving, adopt ethanol, ether hybrid system removing lipoid material wherein;
(4) deproteinated
Adopt trichoroacetic acid(TCA) removing protein wherein;
(5) decolour
Polysaccharide soln color after degreasing, deproteinated is light yellow, and adopt activated carbon decolorizing technology, make polysaccharide soln reach water white transparency, activated carbon dosage is 0.7%, bleaching temperature 60 DEG C, bleaching time 1 hour;
(6) alcohol precipitation
After decolouring, activated carbon filtration is fallen, adopt 95% ethanol by Caulis Sacchari sinensis leaf polysaccharide precipitation out;
(7) lyophilize
Adopt Vacuum Freezing & Drying Technology that the moisture content of Caulis Sacchari sinensis leaf polysaccharide is reduced to less than 5%, Vacuum Freezing & Drying Technology at utmost can keep the activity of polysaccharide;
(8) pulverize
Polysaccharide after freeze-drying is pulverized.
2. the extracting method of a kind of Caulis Sacchari sinensis leaf polysaccharide according to claim 1, it is characterized in that: the preparation of described Crude polysaccharides comprises: Caulis Sacchari sinensis leaf raw material obtains Caulis Sacchari sinensis leaf slag and Caulis Sacchari sinensis leaf juice respectively through cleaning, low temperature rehydration, cold making beating, enzymolysis, separation, Caulis Sacchari sinensis leaf juice through decolouring, ultrafiltration obtains permeate and trapped fluid, trapped fluid through alcohol precipitation, be separated that to be precipitated thing be Crude polysaccharides.
3. the extracting method of a kind of Caulis Sacchari sinensis leaf polysaccharide according to claim 1, is characterized in that: in described decolorization process, activated carbon dosage is 0.7%, bleaching temperature 60 DEG C, bleaching time 1 hour.
4. the extracting method of a kind of Caulis Sacchari sinensis leaf polysaccharide according to claim 1, is characterized in that: in described alcohol precipitation step, ethanol adopts 95% ethanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510903017.2A CN105399851A (en) | 2015-12-09 | 2015-12-09 | Extraction method of sugarcane leaf polysaccharide |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510903017.2A CN105399851A (en) | 2015-12-09 | 2015-12-09 | Extraction method of sugarcane leaf polysaccharide |
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| CN105399851A true CN105399851A (en) | 2016-03-16 |
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| CN201510903017.2A Pending CN105399851A (en) | 2015-12-09 | 2015-12-09 | Extraction method of sugarcane leaf polysaccharide |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114249842A (en) * | 2020-09-25 | 2022-03-29 | 广西中医药大学 | Method for extracting sugarcane leaf polysaccharide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911286A (en) * | 2012-11-22 | 2013-02-06 | 广西中医药大学 | Preparation method of sugarcane leaf polysaccharides |
| CN103333265A (en) * | 2013-06-05 | 2013-10-02 | 南方医科大学 | Method for separating and purifying bagasse polysaccharide |
| CN105061618A (en) * | 2015-08-07 | 2015-11-18 | 广西南宁派腾科技有限公司 | Ultrasonic extraction process of saccharum officinarum polysaccharide |
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2015
- 2015-12-09 CN CN201510903017.2A patent/CN105399851A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911286A (en) * | 2012-11-22 | 2013-02-06 | 广西中医药大学 | Preparation method of sugarcane leaf polysaccharides |
| CN103333265A (en) * | 2013-06-05 | 2013-10-02 | 南方医科大学 | Method for separating and purifying bagasse polysaccharide |
| CN105061618A (en) * | 2015-08-07 | 2015-11-18 | 广西南宁派腾科技有限公司 | Ultrasonic extraction process of saccharum officinarum polysaccharide |
Non-Patent Citations (1)
| Title |
|---|
| 桂意云等: ""甘蔗叶片多糖的提取及体外抗氧化作用"", 《西南农业学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114249842A (en) * | 2020-09-25 | 2022-03-29 | 广西中医药大学 | Method for extracting sugarcane leaf polysaccharide |
| CN114249842B (en) * | 2020-09-25 | 2023-07-21 | 广西中医药大学 | Method for extracting sugarcane leaf polysaccharide |
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Application publication date: 20160316 |