CN105039224A - Aquatic microbial preparation using preserved date water as culture medium and preparation method thereof - Google Patents
Aquatic microbial preparation using preserved date water as culture medium and preparation method thereof Download PDFInfo
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- CN105039224A CN105039224A CN201510502831.3A CN201510502831A CN105039224A CN 105039224 A CN105039224 A CN 105039224A CN 201510502831 A CN201510502831 A CN 201510502831A CN 105039224 A CN105039224 A CN 105039224A
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Abstract
The invention provides an aquatic microbial preparation using preserved date water as a culture medium and a preparation method thereof. The aquatic microbial preparation is characterized in that the aquatic microbial preparation comprises the following components in parts by mass: 10 to 20 parts of lactobacillus 1x103cfu/mL, 10 to 30 parts of bacillus subtilis 1x104cfu/mL and 40 to 80 parts of preserved date water. The preparation method comprises the following specific operation steps: taking the preserved date water, performing steam sterilization for 20 to 30min at high temperature of 121 DEG C, cooling to 30 to 37 DEG C, adding the lactobacillus and bacillus subtilis under aseptic conditions, agitating for 10 to 20min, continuously increasing oxygen, performing constant-temperature culture for 24 to 36h, performing concentration detection and sub-packaging the preparation to obtain the microbial preparation after the concentration of lactobacillus reaches 1x107cfu/mL and the concentration of bacillus subtilis reaches 1x107cfu/mL. The preparation has good effects of microbial ecosystem regulation and enhancement of immunity and growth promotion, and is stable in quality, environment-friendly and pollution-free.
Description
Technical field
The invention belongs to aquaculture field, relate to a kind of aquatic products microbial preparation and preparation method thereof, relate generally to a kind of with candied date water aquatic products microbial preparation that is substratum and preparation method thereof.
Background technology
In recent years, along with the continuous expansion propagating scale artificially, breeding environment has the trend increasingly worsened, and the outburst of disease is also more and more frequent, greatly inhibits the growth of aquaculture production and the development of aquatic products trade.Microbiotic, as fodder additives, suppressing the breeding of pathogenic micro-organism, reducing its metabolic toxicities and all serving positive effect to aspects such as the toxic actions of animal.But life-time service microbiotic, particularly abuse of antibiotics, its all drawback also reveals gradually.Simultaneously along with people's living standard improves, the raising of Consciousness of food security, and Post-WTO is to the requirement of Animal by-product more and more specification, when producing and using fishing medicine, needs to consider whether this fishing medicine meets the requirement of food safety essentially.Microbiotic may cause animal gastrointestinal tract normal microflora to be lacked of proper care simultaneously, produces detrimentally affect to animal body immunizing power, produces the side effect such as resistance and drug residue, all brings potential hazard to cultivated animals with as the health of the mankind of products customers.2004, WTO, Food and Argriculture OrganizationFAO (FAO) and OIE (OIE) combined and hold symposium, discuss inhuman antibiotic use and antibiotic drug resistance problems; Therefore, many countries and regions such as European Union, the U.S. limit the use of microbiotic in aquaculture from legislation in succession.The nuisanceless green fishery products of exploitation safety, high-quality, nutrition have become the common recognition of society.
The control utilizing probiotics to carry out aquatic animal disease is a kind of novel method that developed recently gets up, have nontoxic, have no side effect, the advantage of noresidue.Probiotics refers to and adopt known beneficial microorganism, the biotechnological formulation useful to humans and animals made through special process such as cultivation, rejuvenation, fermentation, packaging, dryings or active bacteria formulation under Tiny ecosystem theoretical direction.Aquatic products probiotics develops on the feeding micro-ecological preparation basis of Lu Sheng cultivated animals, China starts from the end of the eighties in last century, the predicament of to be on the point of with disease and to send out is developed rapidly along with aquaculture, after the nineties, the use of aquatic products probiotics in aquaculture is more increasingly general, many raisers are used for water transfer, rich water, change the end, and aquatic products probiotics industry fast development becomes a new industry in aquaculture.The microniological proudcts that are that grow up because probiotics is theoretical based on microecological balance theory, Tiny ecosystem nutrient theory and Tiny ecosystem control, it is a kind of control of active form to the control of fishery products disease.But along with the development of aquatic products probiotics industry and the general of application, it is also increasing in research, application and qualitative problem.In current existing microniological proudcts, bacterial classification is more single, and the synergy between different strain can not well be brought into play.
Summary of the invention
For the problems referred to above that microbial preparation in current aquaculture exists, the object of the present invention is to provide a kind of with candied date water aquatic products microbial preparation that is substratum and preparation method thereof, this microbial preparation has and has that viable bacteria content is high, long quality-guarantee period, have intestinal microflora regulating effect, disease preventing and treating, has the good characteristics of environmental protection simultaneously.
The present invention solves the problems of the technologies described above adopted technical scheme:
The aquatic products microbial preparation being substratum with candied date water, is characterized in that, comprising milk-acid bacteria and subtilis, to be inoculated in candied date water be in same culture medium solution, carries out that fermentor cultivation obtains, wherein the bacterium number 1 × 10 of milk-acid bacteria in said preparation
3cfu/mL; The bacterium number 1 × 10 of subtilis
4cfu/mL.
By such scheme, in described preparation, the percentage composition of the quality of each component is: milk-acid bacteria 10-20 part, subtilis 10-30 part, candied date water 40-80 part.
A kind ofly prepare with the method for the candied date water aquatic products microbial preparation that is substratum, it is characterized in that, its concrete operation steps is: get candied date water 40-80 part, under 121 DEG C of high temperature, steam sterilizing 20-30min, carries out germicidal treatment, after being cooled to 30-37 DEG C, aseptically, milk-acid bacteria 1 × 10 is added
3cfu/mL10-20 part, subtilis 1 × 10
4cfu/mL10-30 part, stirs 10-20min, and continue oxygenation, after constant temperature culture 24-36h, detectable level reaches milk-acid bacteria 1 × 10
7cfu/mL, subtilis 1 × 10
7after cfu/mL, packing, obtains probiotics.
Wherein, subtilis (Bacillussubtilis) is a kind of gram-positive microorganism, and vitality is extremely strong, and metabolism is vigorous, exists with spore state in the ripening stage, has extremely strong anti-adversity ability, promotes zootrophic digesting and assimilating simultaneously; To fly up and down pathogenic microorganism, regulate digestive tube healthy; Stimulating animal immune organ grows, and improves the effects such as immunity of organisms; A large amount of perienzyme can be secreted as proteolytic enzyme, amylase, cellulase, lipase, lecithinase etc., simultaneously also secretion activity antimicrobial substance and Volatile Metabolites, improve food conversion ratio and the utilization ratio of ammonia, reduce the generation of ammonia and Benzazole compounds, the water quality in purifying aquaculture pond, and the absorption that can promote aquatic animal vitamins D.
Milk-acid bacteria: this bacterium can produce some antibacterial substances in process of growth, as organic acid etc., bacteriocin and bacterioid element can suppress harmful microorganism growth and breeding in enteron aisle, as pathogenic colon bacillus and streptococcus aureus; Many unwanted bacterias can be killed, as Candida albicans, intestinal bacteria etc., play the effect of balance intestinal flora; And can vitamin B group be produced, comprise folic acid, vitamin H, vitamin B6 and vitamin K3 etc., secrete various benefit materials, as lactic acid, acetic acid, reduce gut pH.
The invention has the advantages that: the present invention, containing two kinds of high-quality effective bacterium-milk-acid bacterias and subtilis, adopts candied date water to be substratum, as the nutritive medium of bacterial classification simultaneously, ensure the growth environment of bacterial classification, strictly choose seeds by microecology rule simultaneously, breeding, thus ensure bacterial classification excellent, viable bacteria content is high, become current long, and there is good Tiny ecosystem regulate and strengthening immunity, growth promoting function, and steady quality, safety, reliably, green, environmental protection, pollution-free.
Embodiment
Below in conjunction with specific embodiment, concrete introduction is done to the present invention.
Embodiment 1:
A kind of aquatic products microbial preparation that is substratum with candied date water, it is characterized in that, comprising milk-acid bacteria and subtilis, to be inoculated in candied date water be in same culture medium solution, carries out that fermentor cultivation obtains, and in described preparation, the percentage composition of the quality of each component is: milk-acid bacteria 1 × 10
3cfu/mL15 part, subtilis 1 × 10
4cfu/mL20 part, 65 parts, candied date water.
A kind ofly prepare with the method for the candied date water aquatic products microbial preparation that is substratum, it is characterized in that, its concrete operation steps is: get 65 parts, candied date water, under 121 DEG C of high temperature, steam sterilizing 20-30min, carries out germicidal treatment, after being cooled to 30-37 DEG C, aseptically, milk-acid bacteria 1 × 10 is added
3cfu/mL15 part, subtilis 1 × 10
4cfu/mL20 part, stirs 10-20min, and continue oxygenation, after constant temperature culture 24-36h, detectable level reaches milk-acid bacteria 1 × 10
7cfu/mL, subtilis 1 × 10
7after cfu/mL, packing, obtains probiotics.
Embodiment 2:
A kind of aquatic products microbial preparation that is substratum with candied date water, it is characterized in that, comprising milk-acid bacteria and subtilis, to be inoculated in candied date water be in same culture medium solution, carries out that fermentor cultivation obtains, and in described preparation, the percentage composition of the quality of each component is: milk-acid bacteria 1 × 10
3cfu/mL15 part, subtilis 1 × 10
4cfu/mL25 part, 60 parts, candied date water.
Embodiment 3:
A kind of aquatic products microbial preparation that is substratum with candied date water, it is characterized in that, comprising milk-acid bacteria and subtilis, to be inoculated in candied date water be in same culture medium solution, carries out that fermentor cultivation obtains, and in described preparation, the percentage composition of the quality of each component is: milk-acid bacteria 1 × 10
3cfu/mL10 part, subtilis 1 × 10
4cfu/mL30 part, 60 parts, candied date water.
Embodiment 4:
A kind of aquatic products microbial preparation that is substratum with candied date water, it is characterized in that, comprising milk-acid bacteria and subtilis, to be inoculated in candied date water be in same culture medium solution, carries out that fermentor cultivation obtains, and in described preparation, the percentage composition of the quality of each component is: milk-acid bacteria 1 × 10
3cfu/mL20 part, subtilis 1 × 10
4cfu/mL10 part, 70 parts, candied date water.
Embodiment 5:
A kind of aquatic products microbial preparation that is substratum with candied date water, it is characterized in that, comprising milk-acid bacteria and subtilis, to be inoculated in candied date water be in same culture medium solution, carries out that fermentor cultivation obtains, and in described preparation, the percentage composition of the quality of each component is: milk-acid bacteria 1 × 10
3cfu/mL10 part, subtilis 1 × 10
4cfu/mL10 part, 80 parts, candied date water.
Wherein implement the preparation method of the microbial preparation of 2,3,4 and 5 with embodiment 1.
The present invention is not limited to above-mentioned embodiment, and those skilled in the art also can make multiple change accordingly, but to be anyly equal to the present invention or similar change all should be encompassed in the scope of the claims in the present invention.
Claims (4)
1. with the aquatic products microbial preparation that candied date water is substratum, it is characterized in that, comprising milk-acid bacteria and subtilis, to be inoculated in candied date water be in same culture medium solution, carries out that fermentor cultivation obtains, wherein the bacterium number 1 × 10 of milk-acid bacteria in said preparation
3cfu/mL; The bacterium number 1 × 10 of subtilis
4cfu/mL.
2. a kind of aquatic products microbial preparation that is substratum with candied date water according to claim 1, it is characterized in that, in described preparation, the percentage composition of the quality of each component is: milk-acid bacteria 10-20 part, subtilis 10-30 part, candied date water 40-80 part.
3. a kind of aquatic products microbial preparation that is substratum with candied date water according to claim 1, it is characterized in that, in described preparation, the percentage composition of the quality of each component is: milk-acid bacteria 15 parts, subtilis 20 parts, 65 parts, candied date water.
4. one kind as described in claim 1,2 or 3 with the preparation method of the candied date water aquatic products microbial preparation that is substratum, it is characterized in that, its concrete operation steps is: get candied date water 40-80 part, under 121 DEG C of high temperature, steam sterilizing 20-30min, carries out germicidal treatment, after being cooled to 30-37 DEG C, aseptically, milk-acid bacteria 1 × 10 is added
3cfu/mL10-20 part, subtilis 1 × 10
4cfu/mL10-30 part, stirs 10-20min, and continue oxygenation, after constant temperature culture 24-36h, detectable level reaches milk-acid bacteria 1 × 10
7cfu/mL, subtilis 1 × 10
7after cfu/mL, packing, obtains probiotics.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105925515A (en) * | 2016-07-14 | 2016-09-07 | 贝嘉美（天津）生物技术研发有限公司 | Method for culturing lactic acid bacteria by using candied jujube wastewater as medium |
Citations (3)
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|---|---|---|---|---|
| CN103695352A (en) * | 2013-12-17 | 2014-04-02 | 宋青春 | Method for manufacturing water-quality treating agent specific to waste water of Chinese date processing plant by adopting microbial fermentation process |
| CN104673775A (en) * | 2013-12-03 | 2015-06-03 | 青岛碧水蓝天生物技术有限公司 | Micro-ecological preparation for repairing aquiculture water environment and preparation method of micro-ecological preparation |
| CN104671426A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Culture water body cleaning agent |
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- 2015-08-14 CN CN201510502831.3A patent/CN105039224A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104671426A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Culture water body cleaning agent |
| CN104673775A (en) * | 2013-12-03 | 2015-06-03 | 青岛碧水蓝天生物技术有限公司 | Micro-ecological preparation for repairing aquiculture water environment and preparation method of micro-ecological preparation |
| CN103695352A (en) * | 2013-12-17 | 2014-04-02 | 宋青春 | Method for manufacturing water-quality treating agent specific to waste water of Chinese date processing plant by adopting microbial fermentation process |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105925515A (en) * | 2016-07-14 | 2016-09-07 | 贝嘉美（天津）生物技术研发有限公司 | Method for culturing lactic acid bacteria by using candied jujube wastewater as medium |
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Address after: No. 28 Samsung Road, Zhongbei Industrial Park, Xiqing District, Tianjin 300000 Applicant after: Bei Jiamei (Tianjin) Biotechnology Research and Development Co., Ltd. Address before: 300 000 Area A, No. 28 Samsung Road, Zhongbei Industrial Park, Xiqing District, Tianjin Applicant before: Bei Jiamei (Tianjin) Biotechnology Development Co., Ltd. |